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<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGN6</accession>
    <name>I6ZGN6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="3">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="3"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263845.1">
        <property type="nucleotide sequence ID" value="XM_009265570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGN6"/>
    <dbReference type="GeneID" id="20520629"/>
    <dbReference type="KEGG" id="ero:EROM_010030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010030"/>
    <dbReference type="HOGENOM" id="CLU_031243_0_0_1"/>
    <dbReference type="OrthoDB" id="36455at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033379">
        <property type="entry name" value="FrucBisAld_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="333" mass="37414" checksum="F3CD562B9305671B" modified="2012-10-03" version="1">MDCDHLLKLGMMAKKILENGKGILAADESPGTLGKRLERLGIENTEENRRKFREILFSTKELERYIGGVILNQETFGQTNEDGVPLTKLLKKRGIEIGIKLDKGLIDYKEKEKISVGLEDLDLRCKSSTFKDATFAKWRSLFYFYDGIPSEDCINENCSVLAKYAIICQKNGLVPIVEPEIFLEGDYTIEGSYKVFRQILSTLVKYLNYESVYIPGVLIKTSYVTSGILSDEKYTPKKVATYTFRALLSTVPCGVPGIVFLSGGHSSEKAIAFLNAINMERGCRTWTLSFSFGRALTDDVLEAWRGEDPNIEEAQRVLLETSFKAYKAAEGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH44</accession>
    <name>I6ZH44_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264010.1">
        <property type="nucleotide sequence ID" value="XM_009265735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH44"/>
    <dbReference type="GeneID" id="20520796"/>
    <dbReference type="KEGG" id="ero:EROM_020380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020380"/>
    <dbReference type="HOGENOM" id="CLU_601323_0_0_1"/>
    <dbReference type="OrthoDB" id="2191542at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="455" mass="53064" checksum="E93858520CCB4C7D" modified="2012-10-03" version="1">MQVSYIEKKSVDELSISTLIGDRIFFIRGMELLSKSIYMYDSEVLHKVFKDKVDYICYFNGVLLVLCGNALYRYKLFGEELVLSGTHQLLEPPIRICVNETSETKENCGVCFLYRNKKIEILNDEVEKIGVRCKGDVAREDEIIDFSIDGGSSWFLARSGRIYHTHSFVISRTVLLNKPVRMIPEPTYFDGSINRIMARKGKMYVCYDEGFEMYDIGKDVLVLNYTYRTQDFELHASSDVYCLEKNLLVVNEVPRVIASVRVHKMFGDVGISRDRILFVKRSKRDERTDIRLLKEEDAEVRTREMFRVVENEIRIPEIELSENGKDASEKSFLMIQKMINEFESKVLDVYRRIYFELVTLNESFSEKIIGLTAENDLILRKVEVLDKKKNELMARLQRLGEKMSSTVSRVKIDGSRMKRLAKMVEVAIDGTGFKRYEKYSKILKLQREVLNRKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHQ0</accession>
    <name>I6ZHQ0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264215.1">
        <property type="nucleotide sequence ID" value="XM_009265940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHQ0"/>
    <dbReference type="GeneID" id="20521008"/>
    <dbReference type="KEGG" id="ero:EROM_030970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030970"/>
    <dbReference type="HOGENOM" id="CLU_086720_0_0_1"/>
    <dbReference type="OrthoDB" id="2189670at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="32875" checksum="7A43D45B88F62145" modified="2012-10-03" version="1">MDEAVEICFRETMSEMTRLVDRNGEHFLLCGLDQEILAMVIKSLKRHAERISRTVVDIHPTTPKDENPDGKVFFIDINHEIPISYQLHIYHYLEKTRRHSCCLVLVSTSCTSVDRLERRVKSRFNHRVFFIGFLPFESYRALYSQLTGIDEDEEIQRQYSIDPSISALSKKIIIKKYELPEYTIETLYTLFNPIHIALMLVAFRKRIKYINCVSEFRTFTSDVNELKGATSMEILFYFLDILDSGLIDKEGCLLVDAYSFKKHVSNTRPLYLRNLMYKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHT4</accession>
    <name>I6ZHT4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_031410</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264260.1">
        <property type="nucleotide sequence ID" value="XM_009265985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHT4"/>
    <dbReference type="GeneID" id="20521055"/>
    <dbReference type="KEGG" id="ero:EROM_031410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031410"/>
    <dbReference type="HOGENOM" id="CLU_014831_1_0_1"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF1">
        <property type="entry name" value="FIZZY-RELATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="6">
        <location>
            <begin position="66"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="188"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="325"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="369" mass="41533" checksum="08E96579B55646CD" modified="2012-10-03" version="1">MDRFLINKKEAMPRYAPRGVYGLGIKETMHEVGFCYPLDESPYLSTIEGQSLGPKIIIQPYRQLKVKSLPDDFYSSLIDWSGENVFFTADSCLFVHNFFSSKTLQVCNLSSLGITSVKCNPTTSTVALGTSIGVMLSVDMGSLKMTRYSFHRSRIGVIESENTNIITGSRDRKIKITDLRSEKAVATILNHRQEVCGLSISKDLRFLASGGNDNRLYIYDYRNLAHPLKQCSNHKAAVKAISWSPLSPNLLVSGGGTADKTIKLWDISLINSSRPSPCLIRSVDYGSQICNLRWLKSNKILSTHGYSKDDIRLSQMSSFKVERYFLGHKNRVIHFSCSDDEKYFVSGSSDSNINFWELDGEKDDEIKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI53</accession>
    <name>I6ZI53_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CutA-like periplasmic divalent cation tolerance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CutA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264400.1">
        <property type="nucleotide sequence ID" value="XM_009266125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI53"/>
    <dbReference type="GeneID" id="20521200"/>
    <dbReference type="KEGG" id="ero:EROM_041370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041370"/>
    <dbReference type="HOGENOM" id="CLU_098807_2_0_1"/>
    <dbReference type="OrthoDB" id="2017693at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010038">
        <property type="term" value="P:response to metal ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004323">
        <property type="entry name" value="Ion_tolerance_CutA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419">
        <property type="entry name" value="DIVALENT CATION TOLERANCE CUTA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23419:SF8">
        <property type="entry name" value="FI09726P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03091">
        <property type="entry name" value="CutA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="106" mass="12237" checksum="8ACA2B9FEA03D52E" modified="2012-10-03" version="1">MPSLLIVFTTYPTKESAEKVSYELLARKLAVCCQISQVASIYSWKGSVEKETEYKLTIKTSSSLFDKIQRFMEESHPYEVPEIIGIEARSASKEYLEWLHLQIDNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI95</accession>
    <name>I6ZI95_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264440.1">
        <property type="nucleotide sequence ID" value="XM_009266165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI95"/>
    <dbReference type="GeneID" id="20521242"/>
    <dbReference type="KEGG" id="ero:EROM_050090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050090"/>
    <dbReference type="HOGENOM" id="CLU_420346_0_0_1"/>
    <dbReference type="OrthoDB" id="2191971at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="553"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="630"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="656" mass="75712" checksum="95E750518DEB1BC2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZID5</accession>
    <name>I6ZID5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">B-related factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_050500</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264480.1">
        <property type="nucleotide sequence ID" value="XM_009266205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZID5"/>
    <dbReference type="GeneID" id="20521282"/>
    <dbReference type="KEGG" id="ero:EROM_050500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050500"/>
    <dbReference type="HOGENOM" id="CLU_010293_3_0_1"/>
    <dbReference type="OrthoDB" id="511529at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20553">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20554">
        <property type="entry name" value="CYCLIN_TFIIIB90_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.650">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011665">
        <property type="entry name" value="BRF1_TBP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF4">
        <property type="entry name" value="TRANSCRIPTION FACTOR IIIB 90 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07741">
        <property type="entry name" value="BRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="13">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="395" mass="44621" checksum="4CF7A0FDC21AAE5D" modified="2012-10-03" version="1">MGICSNCSSGDFAEDTAQGAVYCTTCGMVQEENAIVASLNFNTEGPKATLNGQIVGIDSRNVGTGFVDSSYYIKNTISGICASLGLGIDHVECSFRWYKLLLQYNLSKGKSILYTLSACIYIVCRQEKTPHMLMDFSNALHIDVFKIGKSFLKITTMLGIDIPLIDPSLYMPRFVSRLRFESSEVLGLSLRLISRMKRDWIVVGRRPNNLCGAALLIASRIVGEERSIYEIAKIVHVSVSTINKRLKEIGDTESANLSIEEFNATWIDKEEDPPSVKLRRMEMAKKQECESGSTETVPYSTPQSSIDSDILSDSEEIERNILSPEETKRREIVWEEMYGEFMREREKRVKSSVKKGKRRRRNEEFGSIVEAFRSLDRKISGKLNYQAIESIFDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIF0</accession>
    <name>I6ZIF0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="3 9">5.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_050660</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="3 9"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264495.1">
        <property type="nucleotide sequence ID" value="XM_009266220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIF0"/>
    <dbReference type="GeneID" id="20521298"/>
    <dbReference type="KEGG" id="ero:EROM_050660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050660"/>
    <dbReference type="HOGENOM" id="CLU_017947_3_1_1"/>
    <dbReference type="OrthoDB" id="5831190at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023096">
        <property type="entry name" value="G6P_Isomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001211">
        <property type="entry name" value="PRK00179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="5 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="507" mass="57934" checksum="BAE9614E5B7F7405" modified="2012-10-03" version="1">MKNEIMNLFEEDKDRARRFARKAEIGNEFIYYDFSKTHLTEEIIDGYLEKMKDFGKKIDGMFSGEKINFTEDRKVLHVALRDKEVLRMVDGGKDVKLDEDRRMVYDELMKIKRFVEDFDNGRICGVTGKRLATIVNIGIGGSDLGPRMVCDALSHYGRKGMETYFISNIDATDTIKVFEKIDPERALFIVVSKTFTTLETIKNAELAMKFLEKRLGKSKSEIASKHFVAVSSNVEEVKKHSISRVFSMWDFVGGRFSLWSAVGMSIALYIGFDNFMRLLKGASAVDEEFRKNKGKSNAEMVHAIVEIFYSENGYNNKCLVSYDQYMEKFYLYLQQAEMESNGKQSERGDTGLIIWGGLGTNTQHSFFQLLHQGTRKILVEFLMPLKPLHSEEEYHKLVVSNCLAQSRGLMVGKKESTPEKSFEGNKPSITICYSMLTPETLGGMIAHYEHKIFILGLYWGINSFDQPGVTLGKKIAIEVLETLESKEDGKYDGSTNEILCLIRRCRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJL2</accession>
    <name>I6ZJL2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264930.1">
        <property type="nucleotide sequence ID" value="XM_009266655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJL2"/>
    <dbReference type="GeneID" id="20521746"/>
    <dbReference type="KEGG" id="ero:EROM_080100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080100"/>
    <dbReference type="HOGENOM" id="CLU_049633_4_0_1"/>
    <dbReference type="OrthoDB" id="10034502at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013744">
        <property type="entry name" value="SidJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31591:SF1">
        <property type="entry name" value="UPF0613 PROTEIN PB24D3.06C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08538">
        <property type="entry name" value="DUF1749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="30819" checksum="270F0ECC8F8AD4CF" modified="2012-10-03" version="1">MYKEELEGKIFVYDPANNLIAYSNQVKSDTKIIYVGGLGSRLPICLPTTMLNEHCFENGYELIIPELRSYPNYGLFTIDDDVEDLSCLLHQLRGNIVLIGNSTGCQDIIHYLNTTRDKRIKLAVLLGAVSDVEFEEHKNKDLPETLRWAKEMTDRKEDVAIKYCGTDSYLTPRRILDIFSRYGKEDMFSSYLEDRFYMELNRGDTRLLFVVSGKDEYSIRDIEAKLRLVRNSRVEKIPEGTHVLSRVEDVKVFLRFFSEEIKKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJP0</accession>
    <name>I6ZJP0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">cysteine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cysteinyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_080410</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264960.1">
        <property type="nucleotide sequence ID" value="XM_009266685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJP0"/>
    <dbReference type="GeneID" id="20521777"/>
    <dbReference type="KEGG" id="ero:EROM_080410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080410"/>
    <dbReference type="HOGENOM" id="CLU_013528_0_3_1"/>
    <dbReference type="OrthoDB" id="438179at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00672">
        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000027">
        <property type="entry name" value="Cysteine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1910">
        <property type="entry name" value="Cysteine-tRNA ligase, C-terminal anti-codon recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00041">
        <property type="entry name" value="Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015803">
        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024909">
        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032678">
        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890:SF3">
        <property type="entry name" value="CYSTEINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="CYSTEINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA synthetases class I catalytic" evidence="11">
        <location>
            <begin position="20"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="482" mass="56225" checksum="5B15FA0FA30D7F1E" modified="2012-10-03" version="1">MTMDRSLKLYNSITKTKETFVPSRGNEVKMYICGPTVYDSPHIGHARTYVMFDVMRRVLSDYLKYDVRFVMNITDIDDKIISRANVSGASTEEITRKYTEEFFEDMEALNVRQPSFVTFVTSYVDKIVKFIEKLETNGFAYESGGSVYFDLEKYQEKHNYPLFKSKDGINSEGAENKDKRGSWDFVLWKKSKENEPRYKSKWGYGRPGWHIECSVMSSDILGGDLDIHAGGIDLAFPHHENEIAQCQAYFMKEPWVRWFLHTGHLNIDGLKMSKSLKNFTTIKEALEMISPRQLRVLFLYHQWNKDMSYEREHLKFAESIEKKIFNFMSIAESMRKNTLGFKTLEDVDREVLEELKDVQESIHAAILDNIDTPTVMRKVVEMINFTNIRIKGISPSTILVVKDYIKEIMEMFGLSEGEAQASSKEDLVAQMLSDFRESIRGMARRKEPYSNFFQKCDWVREAIKDYGYIIEDNNEGSILRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJU4</accession>
    <name>I6ZJU4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 domain-containing putative guanine nucleotide binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_081120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265025.1">
        <property type="nucleotide sequence ID" value="XM_009266750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJU4"/>
    <dbReference type="GeneID" id="20521847"/>
    <dbReference type="KEGG" id="ero:EROM_081120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081120"/>
    <dbReference type="HOGENOM" id="CLU_831632_0_0_1"/>
    <dbReference type="OrthoDB" id="7875889at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF8">
        <property type="entry name" value="F-BOX AND WD REPEAT DOMAIN CONTAINING 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="63"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="150"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="4">
        <location>
            <begin position="201"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="334" mass="36919" checksum="E51537B1AAAF34CE" modified="2012-10-03" version="1">MTSAELEVLEYLNGHKDEVKGLKIVRVSGREILYSSSKDKKVFSWDLHSKIESEFGKILKLYEGGHSKRINGLDVSKDGRMMVTVGSDGIGRIWDTEVKKSKRLEGHTRDVLCVSINSNDTKIVSGSVDRTMNLYNTRGELIAKMGKDTEKMHRGWINCVAFHPIEENVLASGSTDGTVKIWDLDSQEHMQTYLGGVYVDYERAKEKKVLPVDYDESKSVTAMAFSKDGSILTYGEKSGKVYLVNLDTKECIQSLDAITPVRSIAVGEAEPVIALGTDDSVIIWETINSRVIASHNLKEIGNGVRCLSLAFSGNALYCGLSTGSIILLELRKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKD2</accession>
    <name>I6ZKD2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA polymerase delta regulatory subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265230.1">
        <property type="nucleotide sequence ID" value="XM_009266955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKD2"/>
    <dbReference type="GeneID" id="20564341"/>
    <dbReference type="KEGG" id="ero:EROM_091170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091170"/>
    <dbReference type="HOGENOM" id="CLU_021763_2_0_1"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="4">
        <location>
            <begin position="12"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="3">
        <location>
            <begin position="170"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="425" mass="48434" checksum="AD80CF0398AAF47C" modified="2012-10-03" version="1">MDDLSLVNYEHQFNRTYDARLAELRPYIIKSAKALNAGPILDSILGIEEEPGNRRCVIIGTLFIASDLKPTIFDKINRKSKKIKEMEAKTYYSQEIKYFLEDGSGRVEVEFLDISWIYQRNFVISTGMCIGVAGAMTERTKFLAEDILFPFSHPQSFPYISRNLNSRQRVCFISGPSISNENKNRERMMVIIDYLRMTGVKEYIMIGNFFAESKEVSKQMLSLLDSVLKYAGGRINLIPNIRDFGSRVLPLSPVHPKLFTLPVGANTNPCHLEVDGRKVLITTRFVVEDLLKYLPQDLENVQGNNFEYKMHLDMEKINEAAPRSMSNEGNIIRALEVLMKAGHVCPTAPDTIQSVPFSGKDPFVVTGQVDYLCVGDTDEFLEGQSENGTTLFFTIPRFSERHEVVVLDMETRILEVVRFDDKDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKE1</accession>
    <name>I6ZKE1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ribosomal RNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_091320</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265245.1">
        <property type="nucleotide sequence ID" value="XM_009266970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKE1"/>
    <dbReference type="GeneID" id="20564356"/>
    <dbReference type="KEGG" id="ero:EROM_091320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091320"/>
    <dbReference type="HOGENOM" id="CLU_009422_1_2_1"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000220">
        <property type="entry name" value="CAMK protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF12">
        <property type="entry name" value="TRNA (CYTIDINE(32)_GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="7">
        <location>
            <begin position="19"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="237" mass="26435" checksum="7D20BB8CB98B2F72" modified="2012-10-03" version="1">MDGDEKRDIYYRLAKKNKYRARSVYKLMNIDEEYNLFQNIENVVDLCAAPGSWSQYACEKLMKKGGNPKVVSVDVQDIIPIEGATCIKDDITSTSCLESILEVLDGPRADLVMCDGAPDITGIHEIDEYLQMELLMSALATSLRISKAGSSFVGKCLQGEYISCMANHFGRFYNKTALLKPKASRTESMECFLYCTGMKAIGIDPYEIDTTIGRNVPILLCGYGKEPGLEYIVNQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKG3</accession>
    <name>I6ZKG3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265275.1">
        <property type="nucleotide sequence ID" value="XM_009267000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKG3"/>
    <dbReference type="GeneID" id="20564386"/>
    <dbReference type="KEGG" id="ero:EROM_091620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091620"/>
    <dbReference type="HOGENOM" id="CLU_863378_0_0_1"/>
    <dbReference type="OrthoDB" id="2190878at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="37670" checksum="0C325318A8E749BE" modified="2012-10-03" version="1">MKLGPKRIKKRCLVFLVLLFNLLLILFVKHKNRMDITNKVDMGRLRWFGTSTMVVNGLEMFQNVRFSYAIEAGDLISIDVQDQGDVVDSILSRVNTEVREVLLIDARRLRGLNRTVFPRFKRERASHSLGADYIEELAKDFIRRVNNGSVKYPQDSSVAKEYVIKLKSFTKKQTKGEDSSEIIRLVNKAFPTINISFYTYIMMILLNIREEIVDDFYAIEESFKCNRIYSISNDKINHLGRMIMKTRVFSDTIPKRDVCDVCCKVFTGEDRANLQRLMCNDIICRRCLKQSFLYLSDEFCVKCKQKYDILSDDFSKKYLIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKG7</accession>
    <name>I6ZKG7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">asparagine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Asparaginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_091670</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asn) + L-asparagine + ATP = L-asparaginyl-tRNA(Asn) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9659"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.22" evidence="3"/>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265280.1">
        <property type="nucleotide sequence ID" value="XM_009267005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKG7"/>
    <dbReference type="GeneID" id="20564391"/>
    <dbReference type="KEGG" id="ero:EROM_091670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091670"/>
    <dbReference type="HOGENOM" id="CLU_004553_2_10_1"/>
    <dbReference type="OrthoDB" id="1931232at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004816">
        <property type="term" value="F:asparagine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006421">
        <property type="term" value="P:asparaginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004522">
        <property type="entry name" value="Asn-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00457">
        <property type="entry name" value="asnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF16">
        <property type="entry name" value="ASPARAGINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594">
        <property type="entry name" value="ASPARTYL/LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="148"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="451" mass="52211" checksum="5AB6ED4CC59395F5" modified="2012-10-03" version="1">MSEKIEKDVEEQISGLDLSEYKNIELSKITKKDIGQKIRTFGWVSNVRSQSAITFIELYAHYKTVKCVYQKKMHLTICTSMTVYGTVSKNFGKKDAHEFEIQVEGVEIYNEAIAPSFPLNEESSVNAILTNGHLALRTKKRQLFLKARSHLLKIIRDFYFEREYTEITPPTMVQTQVEGGSTLFKLDYYGADAYLTQSSQLYLETVVPVSHRAYCIMPSYRAEKSRTRRHLSEYTHVEAELADVDLNGLISSIEALISYSMKRFYEDMKEDILNVFPNFEFHKVPEVPFKKIKYPEAIELLRSKGYKKEDGADFELGDDIPDAAERYLIEVIGDGLPVFLTHFLVEHKPFYMRKDENDKNLTESTDLLFPGIGEILGGSMRQDTYEDLIEGFKRENISIDPYYWYLDMARFGPCRHGGYGLGFERFLMGLMKYENVDEATLYPRKVSRCQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKK3</accession>
    <name>I6ZKK3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265325.1">
        <property type="nucleotide sequence ID" value="XM_009267050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKK3"/>
    <dbReference type="GeneID" id="20564440"/>
    <dbReference type="KEGG" id="ero:EROM_100100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100100"/>
    <dbReference type="HOGENOM" id="CLU_131641_0_0_1"/>
    <dbReference type="OrthoDB" id="10252174at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.450.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047002">
        <property type="entry name" value="Tcp10_C_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="16071" checksum="49AD837523335D41" modified="2012-10-03" version="1">MTFMQENIKEKIDSIDALMKQLEENRNISVVDILKEEVLKLRKLNEEYRKALEAKKVMHKDQHQNKTRYYLKDGSTYVVKSNQYRYLYDAKTKVITYEFSNGQIEKTFPSGLREIRYPDGSIAIKNGLKDHEYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKL9</accession>
    <name>I6ZKL9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265345.1">
        <property type="nucleotide sequence ID" value="XM_009267070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKL9"/>
    <dbReference type="GeneID" id="20564461"/>
    <dbReference type="KEGG" id="ero:EROM_100310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100310"/>
    <dbReference type="HOGENOM" id="CLU_1875413_0_0_1"/>
    <dbReference type="OrthoDB" id="2194603at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="136" mass="16148" checksum="535BF9E0B19FCDEB" modified="2012-10-03" version="1">MSDEWKGLKIEHVLNHKRTLEWEMIIKQYIRQNSLSLETESEVVRYSDRYFKSSLSEDMVKEIVDGELMPPEISRTFGERKEKKMEDIGEIEGSTEEESVGTEEIEDSNDIENDYVDNFYEDDEELTEEKNEEGFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKR6</accession>
    <name>I6ZKR6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuolar proton pump subunit B</fullName>
            <shortName evidence="5">V-ATPase subunit B</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Vacuolar proton pump subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_100920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265405.1">
        <property type="nucleotide sequence ID" value="XM_009267130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKR6"/>
    <dbReference type="GeneID" id="20564522"/>
    <dbReference type="KEGG" id="ero:EROM_100920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100920"/>
    <dbReference type="HOGENOM" id="CLU_022916_3_0_1"/>
    <dbReference type="OrthoDB" id="1735853at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12240:FF:000001">
        <property type="entry name" value="V-type proton ATPase subunit B, brain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005723">
        <property type="entry name" value="ATPase_V1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01040">
        <property type="entry name" value="V-ATPase_V1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="7">
        <location>
            <begin position="16"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="6">
        <location>
            <begin position="139"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="8">
        <location>
            <begin position="370"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="477" mass="53046" checksum="2F9B4BA7E4344C2B" modified="2012-10-03" version="1">MLESIRIEPKMSYRKIVGVNGPLVIMDNIRFLKYAEIVSLTLPDGSIRQGQVLEVCGKKAVIQVFEGTSGIDVKETQVTFTGETLKMGVSEDILGRVFNGSGKVIDGGPKIIAEDYLDIQGQPLNPYARIYPEEMIQTGISSIDIMNSIARGQKIPIFSASGLPHNEIAAQICRQAGLVKRKDSIDSSDDNFAIVFAAMGVNVETANFFRNSFEASGSIGRTALFLNLANDPTIERIITPRLALTAAEYLAYEKEKHVLVIMTDMSSYADALREVSAAREEVPGRRGYPGYMYTDLSTIYERAGRLEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQIHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHGDVSNQLYAKYAIGKDAQAMKAVVGEDSLSQEDKISIEFLERFEKEFISQRHDELRTVSQSLDIAWDLLKIFPREMLNRIPSDILDEFHSAAPTRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKS0</accession>
    <name>I6ZKS0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chromatin remodeling protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_100970</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ING family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265410.1">
        <property type="nucleotide sequence ID" value="XM_009267135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKS0"/>
    <dbReference type="GeneID" id="20564527"/>
    <dbReference type="KEGG" id="ero:EROM_100970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100970"/>
    <dbReference type="HOGENOM" id="CLU_031900_4_1_1"/>
    <dbReference type="OrthoDB" id="5411773at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15505">
        <property type="entry name" value="PHD_ING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028651">
        <property type="entry name" value="ING_fam"/>
    </dbReference>
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        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10333:SF42">
        <property type="entry name" value="INHIBITOR OF GROWTH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="129"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="site" description="Histone H3K4me3 binding" evidence="8">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR628651-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="183" mass="21297" checksum="7A5B799438596B94" modified="2012-10-03" version="1">MQSHFEVFDKMLEEIQQLRLDAIYFTKLTSRMDGRIERLRRKCKRYKEKFVRKPLERYARKVGKYHKAIRKLLRSNGDSGKTFKSVIEELKKKCEELCSKADVSFDLEGPSIPEMNCVVNLGEMGKNEKPYCRCGRPPFKSMIACDSTDCKRKWFHFECVGISSPPKAPWVCPECRKAATLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKT1</accession>
    <name>I6ZKT1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
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            <taxon>Encephalitozoon</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
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    <dbReference type="OrthoDB" id="2190258at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="515" mass="60200" checksum="EA7F08526CFF7B2D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKY7</accession>
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
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        <property type="nucleotide sequence ID" value="XM_009267205.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20564598"/>
    <dbReference type="KEGG" id="ero:EROM_101680"/>
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    <dbReference type="HOGENOM" id="CLU_042822_0_0_1"/>
    <dbReference type="OrthoDB" id="2195370at2759"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="423" mass="49889" checksum="F213EEB5EAE9B6B7" modified="2012-10-03" version="1">MTDLASKISKFTSLDLESYLNNTFQRYEGSVLENEKHSISKIEHEIDTELLRTFLLNSDSLLNILNVYEESYRMVTSMVDLSKELVRCNMDGSVEEEVFVDKELVKKFRLILNNFDDEAGVFATEERYLVHFDTLREKSGWECILVLANDILLIGAVQKGFKKYRLLNAYSYSIAQIQVKDDMLEVKVDPTVYIFEKNKESVEYILRIYQELTYSYEKKVFDDSPKPEIDKELMNYLVFTEQYEYAEANGLFLSPKIVFHDKAEMMRYLTVASKSKGGISSYIFPFLEERFVKGLRKINKIQPLNNFIEDVFKYFNEFLYEQDDLIKELEKIESIKKSGVVLLIEKQLIRCIEILGVRVFSKGYDVKYTDSVLELINQNLKFSKYDFSYLMEHFLKNRSEYRKKYLDSAMKDIEKIVNDMITD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL09</accession>
    <name>I6ZL09_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265505.1">
        <property type="nucleotide sequence ID" value="XM_009267230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL09"/>
    <dbReference type="GeneID" id="20564624"/>
    <dbReference type="KEGG" id="ero:EROM_110250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110250"/>
    <dbReference type="HOGENOM" id="CLU_1660742_0_0_1"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="159" mass="18163" checksum="3082BCFE621F8989" modified="2012-10-03" version="1">MNKQLCEVESLCLGEIRKGNSEVVEMYFGPYISYNPSTENSAFIKAYMLLYHLSTGTKKRFYTTIETVTPTELENPSIKLVIEVDMCMNIGAVERLKNAVERNTIKEFDRFLRDILESQMKIMEFSESSNESSLGIQSQEDKKAIEDAIFIGRNSSANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZRZ8</accession>
    <name>I6ZRZ8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">mRNA 3'-end-processing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_010210</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the cleavage factor I (CF I) involved in pre-mRNA 3'-end processing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CPSF4/YTH1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263863.1">
        <property type="nucleotide sequence ID" value="XM_009265588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZRZ8"/>
    <dbReference type="GeneID" id="20520648"/>
    <dbReference type="KEGG" id="ero:EROM_010210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010210"/>
    <dbReference type="HOGENOM" id="CLU_024513_2_1_1"/>
    <dbReference type="OrthoDB" id="1914176at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000012">
        <property type="entry name" value="cleavage and polyadenylation specificity factor subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045348">
        <property type="entry name" value="CPSF4/Yth1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102:SF24">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23102">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="12">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="10">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="176" mass="20330" checksum="587BE4AC5A779194" modified="2012-10-03" version="1">MGEPVFSFESYVSNKLGLHEEDEVYCIPYQTNSCKDPNCSKQHVKLSTAVVCKHWLRGLCKKGIKCEFMHEYDLSRMPECYFFSSYGECMNPECNYIHIDPNSSSKECPWYNRGFCRNGASCKNKHVRKKLCYNYFLGFCPRGPTCDYGHPKFNINPAREISRSDIIQKPSNLASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS03</accession>
    <name>I6ZS03_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mra1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_010260</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263868.1">
        <property type="nucleotide sequence ID" value="XM_009265593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS03"/>
    <dbReference type="GeneID" id="20520653"/>
    <dbReference type="KEGG" id="ero:EROM_010260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010260"/>
    <dbReference type="HOGENOM" id="CLU_055846_1_3_1"/>
    <dbReference type="OrthoDB" id="269804at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="180" mass="20282" checksum="E49C66877DB7D9FB" modified="2012-10-03" version="1">MSAPLVFVLKGAKLTCESREDDPEFNRSHKAIQLLMDSPLNKSRKIHVYVHTTSNVLIELSHLLEVPESPIEFSDVMSYLLRRLVVKSSDGAVLAKVIKNPIEKHLPPNSTKVVLSSRGCRMSPKDFEPYIERGLVFFINIGKVEKKEGEVTMKVSNFKLSPVSCCTKLTNMFEDMLGVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSL9</accession>
    <name>I6ZSL9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">E2F/DP transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_021260</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264098.1">
        <property type="nucleotide sequence ID" value="XM_009265823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSL9"/>
    <dbReference type="GeneID" id="20520888"/>
    <dbReference type="KEGG" id="ero:EROM_021260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021260"/>
    <dbReference type="HOGENOM" id="CLU_032091_4_1_1"/>
    <dbReference type="OrthoDB" id="1743261at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14660">
        <property type="entry name" value="E2F_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000458">
        <property type="entry name" value="E2F-like (Mammalian transcription factor)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015633">
        <property type="entry name" value="E2F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032198">
        <property type="entry name" value="E2F_CC-MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12081:SF18">
        <property type="entry name" value="TRANSCRIPTION FACTOR E2F2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16421">
        <property type="entry name" value="E2F_CC-MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="8">
        <location>
            <begin position="14"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="22192" checksum="E459F53E7898ABBA" modified="2012-10-03" version="1">MARKSASDRLSSTRSENSLYNLTRRFLKLISMSPDRNVSIHQASIELNVGKRRIYDITNVLEGLGLLSKWSVSNAKWVGGSIDRYILDSEEKENQENISMDPENLLKGDDLDETLCRLNEEISMLSQSEKNLANAYVTYSDLQSLPSLGGNLVFAVKAPSETTMEYPRYEKGFYKLKLSSEKGAISIFYVSDEKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT33</accession>
    <name>I6ZT33_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoribosyl transferase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_040050</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264273.1">
        <property type="nucleotide sequence ID" value="XM_009265998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT33"/>
    <dbReference type="GeneID" id="20521069"/>
    <dbReference type="KEGG" id="ero:EROM_040050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040050"/>
    <dbReference type="HOGENOM" id="CLU_485733_0_0_1"/>
    <dbReference type="OrthoDB" id="10248306at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363">
        <property type="entry name" value="HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43363:SF1">
        <property type="entry name" value="HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="3">
        <location>
            <begin position="378"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="561" mass="64145" checksum="0241DB1EC5B011D5" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT89</accession>
    <name>I6ZT89_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Asparagine synthetase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_040780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264343.1">
        <property type="nucleotide sequence ID" value="XM_009266068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT89"/>
    <dbReference type="GeneID" id="20521142"/>
    <dbReference type="KEGG" id="ero:EROM_040780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040780"/>
    <dbReference type="HOGENOM" id="CLU_012368_1_0_1"/>
    <dbReference type="OrthoDB" id="10252281at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051857">
        <property type="entry name" value="Asn_synthetase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45937:SF1">
        <property type="entry name" value="ASPARAGINE SYNTHETASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="230"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Asparagine synthetase" evidence="4">
        <location>
            <begin position="378"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="472" mass="54594" checksum="EF9696CFFCD17DBA" modified="2012-10-03" version="1">MCGILLSEEENSAWEEIALLVRNRGEDHYNRIHMGNIVAISSVLAIRESIKQPVVGHRYILLYNGEVYNKEPSDTLFIKSIVDKLLNDGADSCMESTIDRIYEEINRYENEMAMILMIGDYVYFFKDDIGRRSLGYGLSPFYVSSVGYLEEINPMLIYSYNKVTKSLSEWPKSSRIVKKYMSMSDVILKRLNNEKYSKKYQYLKKYLASSGVEANKEDLNNVDDVVKEFGKAFRKSVERRVSEGNICVFFSGGVDSMLVTIFLHYSTDPCQRIYLINTSFGPSWDRDTGKRGFESLCSRFRERAFIFVSNEVSIEEVRAFKEHIYKLIHPKCGRMDFNIGATLFFTARESRKYSKIGYLGSGADEMFGGYHRYKGGEFRGDMLFDLFTISHHNVCRDDRVISDNQVECRFPFLDSGMVEYSLRVGRDILMMNGDRKFVIREVLRREGFECVSKVPKKAMQYGSGIFKMEGKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTA1</accession>
    <name>I6ZTA1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">signal-recognition-particle GTPase</fullName>
            <ecNumber evidence="11">3.6.5.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Signal recognition particle 54 kDa protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_040940</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="11"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264358.1">
        <property type="nucleotide sequence ID" value="XM_009266083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTA1"/>
    <dbReference type="GeneID" id="20521158"/>
    <dbReference type="KEGG" id="ero:EROM_040940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040940"/>
    <dbReference type="HOGENOM" id="CLU_009301_6_1_1"/>
    <dbReference type="OrthoDB" id="10250817at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030942">
        <property type="term" value="F:endoplasmic reticulum signal peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006616">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="13">
        <location>
            <begin position="266"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="466" mass="51147" checksum="518E216E71CC3C64" modified="2012-10-03" version="1">MITELGRSITNTLSNLLSSHTTDQDIDTAIKEICNSLILSNVNPKYVNDLKNSLRAELDPKGMPPGFNKAKMVQNAVYERLVDLLDPKTEGYKIEKGKTNVVVFVGLQGSGKTTSICKYANFYKKKGYKVGIVCADTFRAGAFDQVKQNALKIKVPFFGSSEVDPVKVAGAGVERFRKEKFELILVDTSGRHTQESELFLEMKDIIREVGPNSIVFVMDAGIGQSAEDQAVGFKRAVDVGSILLTKIDGTSKAGGALSSVAATKCPIEFVGTGEGMEDLEMFDARRFVSRMLGMGDVEGLMEKVGSLGIDEKEVVKKLKQGRFTLGDFYDQFQKILSLGPISKLLEMIPGFSGLSLPDEESFKKLIYVFDSMSRGELDSTGQIFEREPTRVVRVARGSGTSVQGVTEILSQFKKVHAMMQKFSSIPGIENMMGNPSSISLSQKAKLKEQAKNILPKDLLDRMSSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTA9</accession>
    <name>I6ZTA9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="4">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_041040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="4"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264368.1">
        <property type="nucleotide sequence ID" value="XM_009266093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTA9"/>
    <dbReference type="GeneID" id="20521168"/>
    <dbReference type="KEGG" id="ero:EROM_041040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041040"/>
    <dbReference type="HOGENOM" id="CLU_033536_13_3_1"/>
    <dbReference type="OrthoDB" id="2603at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000122">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="5">
        <location>
            <begin position="4"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="230" mass="26028" checksum="E3C700113C2310F7" modified="2012-10-03" version="1">MYNVIIPTYNERENIKVLLRMVSDVMKEEGKPFKIIVVDDNSPDGTRVVVESMGLPNVCLLSRKKKLGLGSAYRTALGHCEYPFTIVMDGDLSHDPMYIKTMIELQRKGAEIVVGSRYAEGGGVYGWSMKRKIISLGANNLARTFLNINVSDVTGSFRLYKTEAFRALVEKSVSTGYSFQMELMCLAKRHGFTISECPIVFHDRRRGESKLSLMEIVMYLRTIVLLFFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTC5</accession>
    <name>I6ZTC5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L32</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_041300</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264393.1">
        <property type="nucleotide sequence ID" value="XM_009266118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTC5"/>
    <dbReference type="GeneID" id="20521193"/>
    <dbReference type="KEGG" id="ero:EROM_041300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041300"/>
    <dbReference type="HOGENOM" id="CLU_071479_4_1_1"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="139" mass="16368" checksum="F353C01F7DC798B5" modified="2012-10-03" version="1">MSEELFDIKPLVEIKEAYRKEKKFIRHHSDRYKRVKPSWRKPHGIDSKVRKRCKGQREMPSIKYKKPKEIRHLLPNGLRKVRIFNINDLTPLASLNRFYCGEIAHAVGARKRIAIVNRAKELGICLLNGNARLVQEIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTI2</accession>
    <name>I6ZTI2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Vacuolar ATP synthase subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_050380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264468.1">
        <property type="nucleotide sequence ID" value="XM_009266193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTI2"/>
    <dbReference type="GeneID" id="20521270"/>
    <dbReference type="KEGG" id="ero:EROM_050380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050380"/>
    <dbReference type="HOGENOM" id="CLU_112121_1_0_1"/>
    <dbReference type="OrthoDB" id="10263003at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="208" mass="24187" checksum="FFF7CD5965DE9F40" modified="2012-10-03" version="1">MDRVPSKDIERMMAFINHEADEKIKEMKIKATQEYNTEKARIIKEETFRIENGFVMKQKEIEKKRVMAENSLINTYKQRYLEEKVKILDEIYNEALSICSKEPLNPSLIAQCTEKISGEFVVYCNKKDKKVVLSEYKNAEVREMVSEGVGGVILCSKDCSTIVDNSFASRLETVKSSFEAEINKIIFRYSEQLTLFLSVWIWSSFFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTV8</accession>
    <name>I6ZTV8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="19">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ordered locus">EROM_060110</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="19"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264603.1">
        <property type="nucleotide sequence ID" value="XM_009266328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTV8"/>
    <dbReference type="GeneID" id="20521408"/>
    <dbReference type="KEGG" id="ero:EROM_060110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060110"/>
    <dbReference type="HOGENOM" id="CLU_011312_1_0_1"/>
    <dbReference type="OrthoDB" id="272481at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045951">
        <property type="term" value="P:positive regulation of mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.40:FF:000001">
        <property type="entry name" value="DNA repair helicase (Rad3)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000135">
        <property type="entry name" value="DNA repair helicase RAD3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000128">
        <property type="entry name" value="Putative DNA repair helicase RAD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.20">
        <property type="entry name" value="Bacterial XPD DNA helicase, FeS cluster domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010643">
        <property type="entry name" value="HBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013020">
        <property type="entry name" value="Rad3/Chl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001945">
        <property type="entry name" value="RAD3/XPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042493">
        <property type="entry name" value="XPD_DNA_FeS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00604">
        <property type="entry name" value="rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06777">
        <property type="entry name" value="HBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00852">
        <property type="entry name" value="XRODRMPGMNTD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 22" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="21">
        <location>
            <begin position="7"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="AFN83106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="743" mass="84749" checksum="DD7F818ED3F1ADA1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU99</accession>
    <name>I6ZU99_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated Sec1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264733.1">
        <property type="nucleotide sequence ID" value="XM_009266458.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU99"/>
    <dbReference type="GeneID" id="20521542"/>
    <dbReference type="KEGG" id="ero:EROM_061450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061450"/>
    <dbReference type="HOGENOM" id="CLU_013933_1_0_1"/>
    <dbReference type="OrthoDB" id="10266265at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
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        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="489" mass="57687" checksum="B8F7600CBA84AA56" modified="2012-10-03" version="1">MHDDLLSKKVDEILGMGEGIKALLCDEDTKIILSNVIPHSKFLENDYFLFDNIMNRRREKIQGITCVVVIRPENIRWLIEEVASPFYERYIVLFTNQIDSLMLEILATSDVYCVISEVHEIYMDFFKQDDFLYTFHRAKTSDFTSPSMRKRALDGIFSLIMNLGGIPTIRVQTGDKYLLEDSDVLNTRLTGLNLEQGGTLIMLDRSFDLYTPLLYEWRYQSLLHEHADYENGVVRIGKKSYSVLDDSFFNASKFKDIYEVSEDIKGLIKKAEFKKKRLHEFIFDDLEENTRLSRQLEAHLSQHGHVMKACLRLKDLSEMEMNILKNNKVGREEIDEYLVRKDVSIIERSKLLIIYSLRNKKNPNDEVKRYPDLIDEVESFTKRYPLGMPIWRYYGYRFDDDVDIKLGYQPAVKRVLRHWWTNRLDGKYFSTVRESENPMNYIIVYVRNGITYSEYRALCEYYNIAMKGRSRLYVVGDSMVSYKNIMKGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUL7</accession>
    <name>I6ZUL7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264893.1">
        <property type="nucleotide sequence ID" value="XM_009266618.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUL7"/>
    <dbReference type="GeneID" id="20521709"/>
    <dbReference type="KEGG" id="ero:EROM_071450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071450"/>
    <dbReference type="HOGENOM" id="CLU_353368_0_0_1"/>
    <dbReference type="OrthoDB" id="2196350at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="795" mass="84522" checksum="1754634CA981B6DC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUU0</accession>
    <name>I6ZUU0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rad1-like DNA repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_080720</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264988.1">
        <property type="nucleotide sequence ID" value="XM_009266713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUU0"/>
    <dbReference type="GeneID" id="20521808"/>
    <dbReference type="KEGG" id="ero:EROM_080720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080720"/>
    <dbReference type="HOGENOM" id="CLU_002265_2_0_1"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000002">
        <property type="entry name" value="DNA repair endonuclease XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="10">
        <location>
            <begin position="546"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="768" mass="88453" checksum="375556B79867BBD3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV91</accession>
    <name>I6ZV91_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase III subunit RPC3</fullName>
            <shortName evidence="1">RNA polymerase III subunit C3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265138.1">
        <property type="nucleotide sequence ID" value="XM_009266863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV91"/>
    <dbReference type="GeneID" id="20521962"/>
    <dbReference type="KEGG" id="ero:EROM_090240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090240"/>
    <dbReference type="HOGENOM" id="CLU_669209_0_0_1"/>
    <dbReference type="OrthoDB" id="2190900at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013197">
        <property type="entry name" value="RNA_pol_III_RPC82-rel_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039748">
        <property type="entry name" value="RPC3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12949">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08221">
        <property type="entry name" value="HTH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit RPC82-related helix-turn-helix" evidence="2">
        <location>
            <begin position="4"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="418" mass="47996" checksum="27D15F5798C02F28" modified="2012-10-03" version="1">MPGLVGEILGDYGTILQKIGLFLRKRNGSTMEYITQGTSLAYSQVIHGLSLMIQRRLVKYFQYEKKVHYVLDTDMAYRRMYFGTYCSLVEDLFGEEVAEGFMKILVNGFTKVNISLEKKVEELVSAGVIISISMKEAGILVAESKNLGEHMTRDRQDKVNEEVHRKSRKTVERARFHVIDFDALHAMMINNRLLALVKKRYLSKAERIYKSILGRNLVTVDAIIESLEDEADITRGDVVSCIKYFNNCGLVRKSMDCTETYFKDEDDIRKILVVDTLNRILSENSKEARRIFNMMLEYKSLEDKDIVIKSLVPSHIVKKAVLMLHSNGFMVLKHTGAGESRPVLEWQVDIDRVSRIVSEEIKRVLEESWTLINQRWGYIGSNGDGEDGGRKELERMLGLSLDFFVLGIRNISFKSEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVG8</accession>
    <name>I6ZVG8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Splicing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265223.1">
        <property type="nucleotide sequence ID" value="XM_009266948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVG8"/>
    <dbReference type="GeneID" id="20564334"/>
    <dbReference type="KEGG" id="ero:EROM_091100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091100"/>
    <dbReference type="HOGENOM" id="CLU_733672_0_0_1"/>
    <dbReference type="OrthoDB" id="187617at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039726">
        <property type="entry name" value="Prp40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864">
        <property type="entry name" value="PRE-MRNA-PROCESSING PROTEIN PRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11864:SF0">
        <property type="entry name" value="PRP40 PRE-MRNA PROCESSING FACTOR 40 HOMOLOG A (YEAST)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="1">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="377" mass="45513" checksum="92F073EE6A2B32CA" modified="2012-10-03" version="1">MRAVESRLDYEILVAPDNRTYYYNRRTRKSSFTKPDILKSSDEEFEVDPWIECRSKQGKTFYHNTITGESRWKRPLERKGEKALIRGIGIRMKITDKETSRYLLHRLLEQYSVENMKDAFYKLSTEPIFRAMEVNTREWLMRKYFEDKEKDAREEEARRQKYYVDEVCKIDVNVSDFFGFNNVFSKHPYYLKIEDKFGCYEVYVERHMGGTTARRLEKIFKGLGIDLHSSIEDVLKMKEVESFDRKAVLFSFIRYFRRLESEFLSNIKKKKMEIIQNICYHKEEFKRLLGKMYNEGLIYYKMKFKNAFHLFKDSEPFLNLLGSSESPKEIYFEFINDLENRLRRYEKEGAKEISPVDKRAMDKYFESIGEEKEEGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVK4</accession>
    <name>I6ZVK4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein disulfide isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091550</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265268.1">
        <property type="nucleotide sequence ID" value="XM_009266993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVK4"/>
    <dbReference type="GeneID" id="20564379"/>
    <dbReference type="KEGG" id="ero:EROM_091550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091550"/>
    <dbReference type="HOGENOM" id="CLU_036412_0_0_1"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="312"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="456" mass="50809" checksum="1AD539130EC7BBDC" modified="2012-10-03" version="1">MFSVVFGIALSGYAMGDVAHVQESQLLNNLKIVPSPSMVEILKDMKSVFSYVTGDEKFDGEGIKIKHEGRTFIAQSAEDVEKAMDVLKKYPAPTEGAINAMKSEEVGSKSRQGNFIVYFLDSSGYKENLQSNKEGFEGFVSTDLSLASSLGIPVPGIYGYNSSDKLSYKLELSDENNKRILSLSNLPIFGFTSSANISLYRALSGTIFYIFFDMKNGPEVLAEYSGTLNDFRYDIRVILIPSIKGSIDLGEYGLTEESLPGCMSISEDGGKYILKNVSRENISGFIKDVLDKKAEIFYKSQDEPEDNASRNVKVVTRNNIKTYLEDTSKDRFIVFGTERCPHCIRIKPIIERLGEIVKENANDKVLVGYCDVGMNDMNDFDIQFVPTLLLYKSGGRESVRYSGGERNLLNLVRFIREFGGLGIDLSMFVTPEGEERKFEVGDEADAVKEEDVRAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVU5</accession>
    <name>I6ZVU5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_100550</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265368.1">
        <property type="nucleotide sequence ID" value="XM_009267093.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVU5"/>
    <dbReference type="GeneID" id="20564485"/>
    <dbReference type="KEGG" id="ero:EROM_100550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100550"/>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="192"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="402"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="119"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="797" mass="89660" checksum="DE2B0F3968900157" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVU9</accession>
    <name>I6ZVU9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265373.1">
        <property type="nucleotide sequence ID" value="XM_009267098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVU9"/>
    <dbReference type="GeneID" id="20564490"/>
    <dbReference type="KEGG" id="ero:EROM_100600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100600"/>
    <dbReference type="HOGENOM" id="CLU_1045951_0_0_1"/>
    <dbReference type="OrthoDB" id="2190614at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="266" mass="30723" checksum="EE8D436A9378632F" modified="2012-10-03" version="1">MIYDEKTLLKALEHKNTEERLLFPSICSVLGLQYFSEENGVLAHTFASPSFVIDMCEESAKITFVDESFSKCFGYVEFCLSRFLEKRRMVDFYFHLKMFSRMERGNERVSEKGSKDVCHCVYDGNYCINPSFKDVAEASTNGIYNIYRHRFEYHLYTKEFILPIPMNASPEDEKCVSLEGEDVNGRKVNAKISLNLARYFGAKAFSREVLRALPPMGFYWRGEIGCSEVLVQKDLAVYIDGELHRDVAFLMKLGKSLDFSLNFFTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW98</accession>
    <name>I6ZW98_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein S10-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265538.1">
        <property type="nucleotide sequence ID" value="XM_009267263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW98"/>
    <dbReference type="GeneID" id="20564658"/>
    <dbReference type="KEGG" id="ero:EROM_110590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110590"/>
    <dbReference type="HOGENOM" id="CLU_122625_2_0_1"/>
    <dbReference type="OrthoDB" id="10248551at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="3">
        <location>
            <begin position="22"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="120" mass="14199" checksum="1BD7E62ADDCD8D7F" modified="2012-10-03" version="1">MDTEMKKQDIDVGSAMETMKVCIDMASLEEKRINKVCREFYEYGKTFIDNLQREVVLPKKIESITTLRNPSGQGTKKWSRYKMIIHLRNFYLEATHQQLEKVVQFLKNFPHVEINLSIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWE4</accession>
    <name>I6ZWE4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265588.1">
        <property type="nucleotide sequence ID" value="XM_009267313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWE4"/>
    <dbReference type="GeneID" id="20564708"/>
    <dbReference type="KEGG" id="ero:EROM_111090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111090"/>
    <dbReference type="HOGENOM" id="CLU_943423_0_0_1"/>
    <dbReference type="OrthoDB" id="2187403at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707460" evidence="1">
        <location>
            <begin position="24"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="295" mass="33170" checksum="3C30469E1D2BF90B" modified="2012-10-03" version="1">MFLMTPSPLLLLIGLVREGLALASEYNFGGFESDPACTKDDKIYLVSLYAHFDRGTIDAIFSAFSDCKDEREAVEHYLKTRVIGPYNDYLSKFKVKIGFDAKDYDVNELVPEKRFDSSCELVEPVSSRTNTVHERLLNTYKDRKIGIHLFLWSCPSHNENIMPKIVIGEPKECARSIGVLWRGTEDTIDFIKSGISEAISGAKDLFLNQEFPNSKDEEKLSYVCQHVDTCIASKPSVNGAIIFGIDETSDEAPSTHIDVDSHPSEVYIPPEKGFRGQRVIYVNEPSDSADDDCKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACZ3</accession>
    <name>I7ACZ3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cdc3-like septin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_011210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263962.1">
        <property type="nucleotide sequence ID" value="XM_009265687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACZ3"/>
    <dbReference type="GeneID" id="20520748"/>
    <dbReference type="KEGG" id="ero:EROM_011210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011210"/>
    <dbReference type="HOGENOM" id="CLU_017718_8_0_1"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="5">
        <location>
            <begin position="22"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="367" mass="42348" checksum="4FEE466D8E5B6B7A" modified="2012-10-03" version="1">MTQKGIGVSNLPNVKYRSFCKAGIDFNIMLVGSNGLGKTSFINQMLGDSVLSSDPFLKPDDEQQSNDSTKVSDKDGCEEQEDKYFHRNSLINIQISKFFVMEKDFQTRITIAEIDGVGDNVCNHECWVPIVDLIQDNFKDYLEQERRNVRPLIKDKRFHVCLYFLEPNPSHVKLVDIKTMKEISKMCNLIPVVGKSDLLSDIERQECSNRIAEALYRESIDVFHLDVPDKERIGKSEYPFFVIAKSTIPEDNNGCSREYPWGTMILEKVVSNDFYLLVDILIAKNLIKLVEATEIFYDDYKTKEIGRSITNKSGALGEDDKRLTREIQKKIKEDEKTIAEIRQKLIEKKKYYESKLLEISVSHENEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD52</accession>
    <name>I7AD52_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CAS/CSE chromosome segregation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264027.1">
        <property type="nucleotide sequence ID" value="XM_009265752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD52"/>
    <dbReference type="GeneID" id="20520815"/>
    <dbReference type="KEGG" id="ero:EROM_020550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020550"/>
    <dbReference type="HOGENOM" id="CLU_384955_0_0_1"/>
    <dbReference type="OrthoDB" id="2189043at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="718" mass="81865" checksum="39A010BF07B589C0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADN8</accession>
    <name>I7ADN8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nuclear cap-binding protein subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">20 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_030640</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RRM NCBP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264182.1">
        <property type="nucleotide sequence ID" value="XM_009265907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADN8"/>
    <dbReference type="GeneID" id="20520975"/>
    <dbReference type="KEGG" id="ero:EROM_030640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030640"/>
    <dbReference type="HOGENOM" id="CLU_070952_2_1_1"/>
    <dbReference type="OrthoDB" id="201398at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000339">
        <property type="term" value="F:RNA cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027157">
        <property type="entry name" value="NCBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847">
        <property type="entry name" value="20 KD NUCLEAR CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18847:SF0">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="37"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="157" mass="18522" checksum="43B17CD1E45AA36A" modified="2012-10-03" version="1">MTTLEQYFAGEREGYFYREKTFVGTDEEYVEAKKNSSTVFISNIPISVREERIWELFLLCGEVKRVIMGLNRNTLRFCGFCFVEFYTVHSADMAVKYINGFRLDQSPLSIDKDYGFVDGRQYGRGVFGGRVKVDKMYLEDRHQRDGTKRRRQGKFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE12</accession>
    <name>I7AE12_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264352.1">
        <property type="nucleotide sequence ID" value="XM_009266077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE12"/>
    <dbReference type="GeneID" id="20521152"/>
    <dbReference type="KEGG" id="ero:EROM_040880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040880"/>
    <dbReference type="HOGENOM" id="CLU_2277460_0_0_1"/>
    <dbReference type="OrthoDB" id="2191130at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="102" mass="12151" checksum="DC12F7DDDD109E27" modified="2012-10-03" version="1">MLSTMAKMAVAGCAIYKIYRKAEKVMKNDEEYLMRITGYTRGLAERMIDEAKKHSRDKEYELSAEYFYYAYKYLDSSWEYVVTQIDSQEVYKLLHAKKSQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE46</accession>
    <name>I7AE46_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264397.1">
        <property type="nucleotide sequence ID" value="XM_009266122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE46"/>
    <dbReference type="GeneID" id="20521197"/>
    <dbReference type="KEGG" id="ero:EROM_041340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041340"/>
    <dbReference type="HOGENOM" id="CLU_1090000_0_0_1"/>
    <dbReference type="OrthoDB" id="2191689at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="255" mass="30043" checksum="D298A8EB79DE0C0C" modified="2012-10-03" version="1">MEGCSHLSISRGVCTSCGLCLESEYGYMASYALYSFVAPKKMKYLASDKSGRYSKNVKSLVNILNVPGYYDEVKQLLETKRFRDRIGANDKILAIIYNVLRRHQYPISYSDLEPFTEKTSSRFKRILLREFEYVETSLEYLSAIFDRVRDFYLTQGFKGNRSLRDFVSISRSNKNINPYYLCLAYFIKDEDLLNTCKKFELDEIVSMSSLKRILKKIQKAEDPTVVPMMKKRLRIKDNARKYLMKRLQDLPYERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEU1</accession>
    <name>I7AEU1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">aspartate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_060720</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264662.1">
        <property type="nucleotide sequence ID" value="XM_009266387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEU1"/>
    <dbReference type="GeneID" id="20521468"/>
    <dbReference type="KEGG" id="ero:EROM_060720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060720"/>
    <dbReference type="HOGENOM" id="CLU_004553_2_4_1"/>
    <dbReference type="OrthoDB" id="372395at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class II (D/K/N)" evidence="11">
        <location>
            <begin position="5"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="139" mass="16079" checksum="E6E88E20E70C9162" modified="2012-10-03" version="1">MKYEDDFSSENEKKLGSIVERMHGVDIFVIKDYPICTRPFYTYRDEEKGVTRSYDFILRGEEILSGAQRVNVYKDLVRYVEEHGISPSSLGGYLESFRYGAPPHGGCGIGLERLMKAYFGMGDIRRFSLFPRDPNRLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEX5</accession>
    <name>I7AEX5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264707.1">
        <property type="nucleotide sequence ID" value="XM_009266432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEX5"/>
    <dbReference type="GeneID" id="20521514"/>
    <dbReference type="KEGG" id="ero:EROM_061180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061180"/>
    <dbReference type="HOGENOM" id="CLU_188567_0_0_1"/>
    <dbReference type="OrthoDB" id="2187855at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="99" mass="11464" checksum="082E93DD0AE28EAD" modified="2012-10-03" version="1">MKITDLQNEVNHLLFLYFTSIGVIQRDAGLSDVYEKMEELKKEIEDCRKRIIRLIDSEEADVELCSDYREIISEGKEFVKDGVSFLDKLLRRDSGSGNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF07</accession>
    <name>I7AF07_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264747.1">
        <property type="nucleotide sequence ID" value="XM_009266472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF07"/>
    <dbReference type="GeneID" id="20521557"/>
    <dbReference type="KEGG" id="ero:EROM_061600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061600"/>
    <dbReference type="HOGENOM" id="CLU_1001253_0_0_1"/>
    <dbReference type="OrthoDB" id="2192418at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="286" mass="32186" checksum="4705212901C89FA0" modified="2012-10-03" version="1">MTFFSKCKNYILEKWRHIEDAFFIGCIALQYNCDTGDYFTPACIASVAGIYLFIKTIYELFDGFKGDDDKSFTVFDFLKTLLVVVHIFVYFLSFVTICEVTRERGKGGWSYGVDARSLIATQTLYMFLVSQSIMANISTNSVIEVFRGVLGFYEIVTLSSIKNDKDYGKLLDLTYPMIFSFLPFVLGEASSLISKWLFQNKLSEYTPRVAKAITVIAAVMCTVVGIYGVFVMLNVMGNITPVEVKVESEQSTHGIKKAFNRVLRNAVGETIGMLGSMFVTKRVVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFB6</accession>
    <name>I7AFB6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">60S ribosomal protein L37</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_071390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264887.1">
        <property type="nucleotide sequence ID" value="XM_009266612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFB6"/>
    <dbReference type="GeneID" id="20521699"/>
    <dbReference type="KEGG" id="ero:EROM_071390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071390"/>
    <dbReference type="HOGENOM" id="CLU_150908_2_1_1"/>
    <dbReference type="OrthoDB" id="10259236at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="21"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="90" mass="10403" checksum="43B8EA7AFDCF2BCC" modified="2012-10-03" version="1">MSKGTASFGKKNKRNTEMCRRCGRQSYHKQKNSCSSCGYPNSKMRNPGSIKARRRRTVGTGRMRYMKRELRAARNGHKGNPILRALWIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFQ5</accession>
    <name>I7AFQ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265072.1">
        <property type="nucleotide sequence ID" value="XM_009266797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFQ5"/>
    <dbReference type="GeneID" id="20521894"/>
    <dbReference type="KEGG" id="ero:EROM_081590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081590"/>
    <dbReference type="HOGENOM" id="CLU_711791_0_0_1"/>
    <dbReference type="OrthoDB" id="2191486at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="390" mass="45608" checksum="2A33812017B77BB4" modified="2012-10-03" version="1">MEEIEKNVAGKIDSLLYEEALQIIDSTPLTYESGINIYPYKGQILYLQKRYEESATFLRRLMESFPQSIKAKRFLYLSLLALGDFRSSFTIAKECWKDGDYRRSTLIRILAHSLVLRNFDFLESLDAHCDEPLLLSMAYIYSGKLDRAVEIMKTIEDSSNTKMYLDALISVRTKSIYMLDYVRYLNEDIFVTAINCLEKKQRVFEKIENLGCKYVEIKIMHIAKKTLGRNEYENVVKSVSSRNEKGHWCRRDCLLGRLRASREERVFFKKNNIYDATERYADLENIFESLRSGNFSDGLEGLKRIYRLETVSDLEKHLKNGQNTLVLNEIERFYLDHGKKEDACHYSDLSAAAHSLRVRDVIECLSRLMDENMIGEIIELLELDLFNKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG10</accession>
    <name>I7AG10_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265157.1">
        <property type="nucleotide sequence ID" value="XM_009266882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG10"/>
    <dbReference type="GeneID" id="20521982"/>
    <dbReference type="KEGG" id="ero:EROM_090430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090430"/>
    <dbReference type="HOGENOM" id="CLU_1085976_0_0_1"/>
    <dbReference type="OrthoDB" id="2190594at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="256" mass="30286" checksum="649A2D07B77AA383" modified="2012-10-03" version="1">MESLINKLNKWHELKKRHSYMLDQKREKEVEAVIEKVKRTRDIEMLLGILATDSDKCKDLEGFLSTEFKRSIGFNSKERINTIIKCMCILDFECERYRLMMIDHLENIYSKIGKASVTERIESLARLKEYDETNGLRIHEYIESRINEEIDRYVERIPVENPKELDGWLNEMVGVCRYRPRVLEMYKGLEIKYFSMCLGIVMMNDKTSALEDTVYLVNKIRRRSAAVGVSIDNEVMGKLNEYEMLWEGDIKALFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG70</accession>
    <name>I7AG70_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265247.1">
        <property type="nucleotide sequence ID" value="XM_009266972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG70"/>
    <dbReference type="GeneID" id="20564358"/>
    <dbReference type="KEGG" id="ero:EROM_091340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091340"/>
    <dbReference type="HOGENOM" id="CLU_1555234_0_0_1"/>
    <dbReference type="OrthoDB" id="660759at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF36">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="19791" checksum="5988271BBA3FB919" modified="2012-10-03" version="1">MDPLMSAFKARESKTFCSPIPHKYEQMFKAKKYDLEHFGLTFFQRAVCFSACLGAGILSFLYSMVKIVRLSPSGFILPYTVSNFLFFIMFGFLLGFRSYLEGLFSKKKRFHSSWFIGCTFLTLYVVLRYDRYLLNLVFCFIQVTSFVMFSLTFVPGGTSGVSSMVNMFFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGA5</accession>
    <name>I7AGA5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative phospholipase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_091740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265287.1">
        <property type="nucleotide sequence ID" value="XM_009267012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGA5"/>
    <dbReference type="GeneID" id="20564398"/>
    <dbReference type="KEGG" id="ero:EROM_091740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091740"/>
    <dbReference type="HOGENOM" id="CLU_036003_0_0_1"/>
    <dbReference type="OrthoDB" id="10265988at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="100"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="5">
        <location>
            <begin position="307"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="555" mass="63114" checksum="B0C587E919E44D9F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGF8</accession>
    <name>I7AGF8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_100330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265347.1">
        <property type="nucleotide sequence ID" value="XM_009267072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGF8"/>
    <dbReference type="GeneID" id="20564463"/>
    <dbReference type="KEGG" id="ero:EROM_100330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100330"/>
    <dbReference type="HOGENOM" id="CLU_030579_0_0_1"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="7">
        <location>
            <begin position="354"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="515" mass="59493" checksum="61E16D5F4B7865CF" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGS2</accession>
    <name>I7AGS2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265482.1">
        <property type="nucleotide sequence ID" value="XM_009267207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGS2"/>
    <dbReference type="GeneID" id="20564600"/>
    <dbReference type="KEGG" id="ero:EROM_101700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101700"/>
    <dbReference type="HOGENOM" id="CLU_818973_0_0_1"/>
    <dbReference type="OrthoDB" id="2194400at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="341" mass="39300" checksum="BB01432B1B495ADD" modified="2012-10-03" version="1">MRTKRKIIILILVFTVLFILSIGVIIKERHLKTSSQTSHGMSILSNSTNKTYDISSDRTLSIEDENLSLNSLNNMPKENEVMDKIDSSFALILREDNGLIRKEDSCNSRNNTESIENKLFKRSPRLISFEVKEKSSTGEYLFSLLRKTIDNICSCSPSGGRKYEKSEITSDHIGLINHYMKSFRILVYKLEVMYSDGFELENIYTPYSDSIDCFTGIKLTRYHTADSFTKNEQRYTSYINQFVDTEAANIPINVEGKGQSCLNVRDHIVYTLLHYVCLFYLKSWKTIDMLYTKLNVEKLSASNVDLSKIINGYKYKDIIETVRPIHFVNTNGCIVRPLIME</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGV4</accession>
    <name>I7AGV4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sn-1,2-diacylglycerol ethanolamine</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_110480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265527.1">
        <property type="nucleotide sequence ID" value="XM_009267252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGV4"/>
    <dbReference type="GeneID" id="20564647"/>
    <dbReference type="KEGG" id="ero:EROM_110480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110480"/>
    <dbReference type="HOGENOM" id="CLU_035066_6_0_1"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF37">
        <property type="entry name" value="BB IN A BOXCAR, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="395" mass="44224" checksum="D5D30475BD1367B7" modified="2012-10-03" version="1">MQGGKFFRMELGPNEISSLRKHKFIGTDNSILSKYVIHHYVKWMLEKVPETIAPNALTLCGFAAMGISLCLTLLFDPYLCNPPRLLSLANFLLMFVYFTCDNLDGAQARRTGTGSPLGQLFDHGVDSYCALITSITLSSTFGFGLSHKFLIFALAIMIQFYLAGVEEKFTGHFVLGKISGASEGIAFALISHLITFACGKGFFQHIFSDGFLWPIKKLYSSILGTTNFSAISVVIATSLVFNTTLTLISIESRTHPSRRFLLYSTFLRIISFTTSFVILYNTLATEGLWTRYLNILMFGQIFSINYVNEVCSYIIKKDLFLFTPVYLMYLAISAALQLQHLKEFQKPLISASFVFSSVYYILVASRVILTLKEALGISFLSIVEDTKKSDEQSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH97</accession>
    <name>I7AH97_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265657.1">
        <property type="nucleotide sequence ID" value="XM_009267382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH97"/>
    <dbReference type="GeneID" id="20564778"/>
    <dbReference type="KEGG" id="ero:EROM_111790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111790"/>
    <dbReference type="HOGENOM" id="CLU_271377_0_0_1"/>
    <dbReference type="OrthoDB" id="2191278at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061863">
        <property type="term" value="F:microtubule plus end polymerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051010">
        <property type="term" value="F:microtubule plus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030951">
        <property type="term" value="P:establishment or maintenance of microtubule cytoskeleton polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046785">
        <property type="term" value="P:microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007051">
        <property type="term" value="P:spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045110">
        <property type="entry name" value="XMAP215"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048491">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12609">
        <property type="entry name" value="MICROTUBULE ASSOCIATED PROTEIN XMAP215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21041">
        <property type="entry name" value="XMAP215_CLASP_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="5">
        <location>
            <begin position="212"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="407"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="422"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="448"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1000" mass="113543" checksum="FC58BFB2940A8FE4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AHA1</accession>
    <name>I7AHA1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Septin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111840</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265662.1">
        <property type="nucleotide sequence ID" value="XM_009267387.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AHA1"/>
    <dbReference type="GeneID" id="20564783"/>
    <dbReference type="KEGG" id="ero:EROM_111840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111840"/>
    <dbReference type="HOGENOM" id="CLU_017718_8_0_1"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="3">
        <location>
            <begin position="36"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="303"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="356" mass="41375" checksum="BC7522B93E5031AE" modified="2012-10-03" version="1">MKNIPTIVKNLGTINVRNPVGFSSVPDQVRESSMIKGFELNVLAVGRRGLGTSTLINSIFAAPLVDKKRSNTITVTRNEIVENDISLEISIVTYHETDISPVIDYINAMNREYFDNEQGLYKAFKDNRIHVCLYLLPSDTLAEQEIKNMYELSQRCNLVPIIPKADMYMPDELIYAKETVRQILSKNNISFFIPYFNENDGDLTEEVADIVENIPFAVIASETMYEHGGEIVRGRKYTWGFINVDLEENNDFKRLQRLLIYTNLDELVTKTDHLFYNNYRKKIFDIENDCGAMKEARYLKLRKETIKILNDKYESRIKALKEEEMEMEKFYTEKLNSLDDKMNEISNQMETSLKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALH6</accession>
    <name>I7ALH6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264026.1">
        <property type="nucleotide sequence ID" value="XM_009265751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALH6"/>
    <dbReference type="GeneID" id="20520814"/>
    <dbReference type="KEGG" id="ero:EROM_020540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020540"/>
    <dbReference type="HOGENOM" id="CLU_182435_0_0_1"/>
    <dbReference type="OrthoDB" id="2195767at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="94" mass="10865" checksum="BF1256D1F860CE67" modified="2012-10-03" version="1">MHFLVKKPGWLVFDPSEYGDEEVKTFQVRHREGRSNTKLVKFKDGSWYLKNGSQMFPLKAVPSRRDIGVGAKEGNVVCIHEVLDKKWFIKMNGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM12</accession>
    <name>I7AM12_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Synaptic transmission and general secretion Sec1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264196.1">
        <property type="nucleotide sequence ID" value="XM_009265921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM12"/>
    <dbReference type="GeneID" id="20520989"/>
    <dbReference type="KEGG" id="ero:EROM_030780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030780"/>
    <dbReference type="HOGENOM" id="CLU_016216_4_0_1"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="521" mass="59804" checksum="737A10A6946B58A2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM39</accession>
    <name>I7AM39_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="2 6">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_031220</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the NMT family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="2 6"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264241.1">
        <property type="nucleotide sequence ID" value="XM_009265966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM39"/>
    <dbReference type="GeneID" id="20521036"/>
    <dbReference type="KEGG" id="ero:EROM_031220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031220"/>
    <dbReference type="HOGENOM" id="CLU_022882_0_1_1"/>
    <dbReference type="OrthoDB" id="60315at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015892">
        <property type="entry name" value="N-myristl_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="9">
        <location>
            <begin position="33"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="10">
        <location>
            <begin position="190"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="355" mass="41315" checksum="710A2972F4C43116" modified="2012-10-03" version="1">MRKNHEFWNTQPVDKNGVGIIPNGTSGSLEQPKLPDGFRFEDLRCIEEVTEFLEKNYVEDVHAEHMLRYSSNFLKWVIDCGGEKKKYCIVLRLCGRMVGFIFGKEHHVVIRGEKNRAIGVNFLCVAQEMRGRRLAPILIKEITRRANVDGIFKGVFTSGTELFFNVSHGRYYHRPLNAENLFMSRFSDRIMKIRETRVRRGTRIAEERDMKEIERLYMEESKRYMLYEEMGSSDIISALKPVKNVVYTYVHEDDGCIDGFGAFLIIETIEKKSRRSVQGGYLYYRGGKDNTKMVEDLIYFAQKEGCDVFNCLDIMENSLFLEGLGFFPGSGELRYYLYNWSSGEVPKDKVFFILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMA4</accession>
    <name>I7AMA4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_040560</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264321.1">
        <property type="nucleotide sequence ID" value="XM_009266046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMA4"/>
    <dbReference type="GeneID" id="20521120"/>
    <dbReference type="KEGG" id="ero:EROM_040560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040560"/>
    <dbReference type="HOGENOM" id="CLU_030988_10_1_1"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23795">
        <property type="entry name" value="UBCc_UBE2G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000051">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 R2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="7"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="172" mass="19627" checksum="ED393C24D7839407" modified="2012-10-03" version="1">MANKGGFSKGLLLKDYKRIQENPNEYYSVGLVDGDIYTWEVLIFGPRATPYENGIFKGRMVFPIDYPGSPPKFRFCSKMWHPNIDENGNVCISILHNPGEDEYGYESLGDRWLPVRTPESVILSIITLLTSPNCESPANVDAAQHLRENEKEYKRKVMELTAMTLDLYDKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMM5</accession>
    <name>I7AMM5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264471.1">
        <property type="nucleotide sequence ID" value="XM_009266196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMM5"/>
    <dbReference type="GeneID" id="20521273"/>
    <dbReference type="KEGG" id="ero:EROM_050410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050410"/>
    <dbReference type="HOGENOM" id="CLU_051701_0_0_1"/>
    <dbReference type="OrthoDB" id="642895at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="269"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="434" mass="52091" checksum="07611FC967E175D9" modified="2012-10-03" version="1">MKSSWPNKLSERIQRLYSTMLTLMPLERIGDHPFDYFDSEIECIIESVDAVFKSILSRRLKMEKEIDGVMERIKKDCGDIEVEAPYVPRLFNLCLLREYVKNESNRITLLKKAVEGRMITIREEIEKIKEDIFDVETMEIDCIELKTMDEESCVSLANLKELEMRRDFLRSKKEGMERNRDRLYEELCVFLSQLSKNDLDVAIGQKIFVLEQLHKKYKEEIERRDLEFRRLEIEIRRREGYLGTPCKEIEMDLSDENLGMMRSYEKYLREEQERQLDEIYEKKRSQLKELLDIFGENMEDYKKTEEGIEEMAMTISKLESKKDLFLSIRSLMEKRSELISKMSEFEKIASDPKRLFRSSLQLLSEEKFRNSAYPNLIRIEEAIFKLLDEYEDRFEKLLKNGIDFKKSLREEIENRIVNKTVFISRFDSPSRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMV3</accession>
    <name>I7AMV3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
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                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="ero:EROM_051440"/>
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    <dbReference type="HOGENOM" id="CLU_055667_0_0_1"/>
    <dbReference type="OrthoDB" id="2192737at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="407" mass="46990" checksum="73B83D6F5F9E4023" modified="2012-10-03" version="1">MCRKTRCVFLLFLSIRLAAVIWEMRFQYLCLEHLKKGQLWFEPGTCTEKMAATTDSFIELQLGASSDRIESMYKEVKRFMLYISVVLLIFNSKFRRRVAELSKSLKISNPEITLLILLISAFTLIEKLCGVKFTLAKYMYSIRYVEPPVGILVLLLIELPLFICLIMKLFELLGRRFIVMFYIIFVVNNLLDTLWCARVDKAKLERIPLNMFPPKLQEIIKSEGMEESIYEVKGSKEKNASQVGLGSFKRIEIYGDFSKDAKKLYPISFHEMGHSIDNIILKRKILLHLIVVCEVLVLSFIYRKESRTFGFSDISSEAFVLIIVVMYLVIGRPLVFIPSNIYAQRSELFADEVAKINGYGRELSQTLLDMTLEHNATIDTTYLFNAITSLHPVARERIDRCGCSPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">EROM_060600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264651.1">
        <property type="nucleotide sequence ID" value="XM_009266376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN52"/>
    <dbReference type="GeneID" id="20521457"/>
    <dbReference type="KEGG" id="ero:EROM_060600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060600"/>
    <dbReference type="HOGENOM" id="CLU_375992_0_0_1"/>
    <dbReference type="OrthoDB" id="2192229at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="744" mass="86239" checksum="5052E198171A90ED" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN82</accession>
    <name>I7AN82_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264691.1">
        <property type="nucleotide sequence ID" value="XM_009266416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN82"/>
    <dbReference type="GeneID" id="20521498"/>
    <dbReference type="KEGG" id="ero:EROM_061020"/>
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    <dbReference type="HOGENOM" id="CLU_811408_0_0_1"/>
    <dbReference type="OrthoDB" id="2193779at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="324" mass="37348" checksum="BF95D45BC93322AB" modified="2012-10-03" version="1">MGEGKVTIIKDKGIFAELLTLVSLFFMITVQFGDINGKVINHIIKLLMFINFLYHVGSCIWRIFTSIQERMSFLNGMFNVRNIVELVTMCILTLYFKEVVTTFDPDKRRMDRCVIVLSMGYLLSKVNEVLDYSTTQSYWSKMIFSTVIILIIGVAAVTDFSDGGVEGPYSEIAYVVGVILAICELAVEGRVVKKSVFKHRRFSVFMAFLLICAISFPAMYPIVNKWKNLFVKWLTERIVIWIKPRMIKVKDKILAVTKEQIRAFISDYTRKVMQEYIPNLKNAIKNRVAPYIPENVKSIPGSIYEWWKRLSGPQQNQPPIGEFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANA8</accession>
    <name>I7ANA8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_061430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264731.1">
        <property type="nucleotide sequence ID" value="XM_009266456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANA8"/>
    <dbReference type="GeneID" id="20521540"/>
    <dbReference type="KEGG" id="ero:EROM_061430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061430"/>
    <dbReference type="HOGENOM" id="CLU_129451_3_1_1"/>
    <dbReference type="OrthoDB" id="10262653at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1740.20">
        <property type="entry name" value="Ribosomal protein S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000892">
        <property type="entry name" value="Ribosomal_eS26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038551">
        <property type="entry name" value="Ribosomal_eS26_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12538:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01283">
        <property type="entry name" value="Ribosomal_S26e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="107" mass="12429" checksum="FEC7A4CEC907FE59" modified="2012-10-03" version="1">MTVKRRNHGRSKKNRGSVKPIQCDKCGRVTPKDKAIKRFRIQSLIEQASFDDLKEKTIYEVFEVPRMGYKSQFCVSCACHAKIVRVRSSQARKIRYGFNPNRASSYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANP5</accession>
    <name>I7ANP5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">tRNA-dihydrouridine synthase</fullName>
            <ecNumber evidence="16">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">EROM_071530</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>5,6-dihydrouridine(16) in tRNA + NAD(+) = uridine(16) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53380"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:53382"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,6-dihydrouridine(16) in tRNA + NADP(+) = uridine(16) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53376"/>
            <dbReference type="Rhea" id="RHEA-COMP:13543"/>
            <dbReference type="Rhea" id="RHEA-COMP:13544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:53378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5,6-dihydrouridine(17) in tRNA + NAD(+) = uridine(17) in tRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53372"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="10">
            <dbReference type="Rhea" id="RHEA:53374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>5,6-dihydrouridine(17) in tRNA + NADP(+) = uridine(17) in tRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53368"/>
            <dbReference type="Rhea" id="RHEA-COMP:13541"/>
            <dbReference type="Rhea" id="RHEA-COMP:13542"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
            <dbReference type="EC" id="1.3.1.88"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:53370"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5,6-dihydrouridine in mRNA + NAD(+) = a uridine in mRNA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69851"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="12">
            <dbReference type="Rhea" id="RHEA:69853"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 5,6-dihydrouridine in mRNA + NADP(+) = a uridine in mRNA + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69855"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:17789"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:69857"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16 18">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Dus family. Dus1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="16"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264901.1">
        <property type="nucleotide sequence ID" value="XM_009266626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANP5"/>
    <dbReference type="GeneID" id="20521717"/>
    <dbReference type="KEGG" id="ero:EROM_071530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071530"/>
    <dbReference type="HOGENOM" id="CLU_013299_5_0_1"/>
    <dbReference type="OrthoDB" id="272303at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106414">
        <property type="term" value="F:mRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102262">
        <property type="term" value="F:tRNA-dihydrouridine16 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102263">
        <property type="term" value="F:tRNA-dihydrouridine17 synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082">
        <property type="entry name" value="TRNA-DIHYDROURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11082:SF5">
        <property type="entry name" value="TRNA-DIHYDROURIDINE(16_17) SYNTHASE [NAD(P)(+)]-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 16 18" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="DUS-like FMN-binding" evidence="19">
        <location>
            <begin position="15"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="17"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AFN83404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="340" mass="39245" checksum="4571F8887D26AFDF" modified="2012-10-03" version="1">MKKFWEEISKPYFAVAPMVGNSEEAWRRLSKRYGANMFYTQMVHCESFLRGPRNPVRNQWYTTSEEDRPLIIQICGNSPEAMLEAALIMQDHCDAIDINFGCPQRVARKGGYGAYLQENWDLTEKIVKTLSTNLSIPVFCKIRVFESIEKTVEYTRMIEKAGCSLLAVHGRTRDQKGKAMGFASWDHIKAVKESLTIPVLSNGNIMVHSDIWRCLEYTKCDGVMVGETHLYNPLIFTGESKPCLEIIREYLDICMEFPGSADVGHMKSHIFKLLYNYFLTNPDRRPIVDSCNTIEKFYKFYLDLVKEMESTINERGTSEVYKMKPRPVLDTNMIDNSKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANU5</accession>
    <name>I7ANU5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264961.1">
        <property type="nucleotide sequence ID" value="XM_009266686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANU5"/>
    <dbReference type="GeneID" id="20521778"/>
    <dbReference type="KEGG" id="ero:EROM_080420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080420"/>
    <dbReference type="HOGENOM" id="CLU_1142589_0_0_1"/>
    <dbReference type="OrthoDB" id="2192781at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27681" checksum="BE46FB7C239EA06A" modified="2012-10-03" version="1">MIFFISTIILESMGDDSSDEIVLAERMDNDSSEISMVEVVRKYQRPTRVKRKGSTQSSQTSKARRRQGVEKAQYADDLSSREPGSDFCEKKELCKVTVVDQNSKKTFHMKKTDTLESIYKAYHEGSSPIKMKYKSVPVSRHLTLEEIGFDGDHCITIHRQGQASGEFGIELKIHVDCTKTIEVNLCKELMIEEVFEKLEEMGFRGDLLVRNGVVLGRKMNIKDVLQTGDVVDLVSKDEIEYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANX6</accession>
    <name>I7ANX6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264996.1">
        <property type="nucleotide sequence ID" value="XM_009266721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANX6"/>
    <dbReference type="GeneID" id="20521816"/>
    <dbReference type="KEGG" id="ero:EROM_080810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080810"/>
    <dbReference type="HOGENOM" id="CLU_020328_0_0_1"/>
    <dbReference type="OrthoDB" id="9991317at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039340">
        <property type="entry name" value="Tfc4/TFIIIC-102/Sfc4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23082">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIIC TFIIIC , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="312"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="609"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="617"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="803" mass="93412" checksum="60D3D9E81865656E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP08</accession>
    <name>I7AP08_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trm112p-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265031.1">
        <property type="nucleotide sequence ID" value="XM_009266756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP08"/>
    <dbReference type="GeneID" id="20521853"/>
    <dbReference type="KEGG" id="ero:EROM_081180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081180"/>
    <dbReference type="HOGENOM" id="CLU_169844_0_0_1"/>
    <dbReference type="OrthoDB" id="2187549at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="123" mass="13979" checksum="0E0215C262FCD014" modified="2012-10-03" version="1">MKPFLVGLLKCKRCSFMTRLVLQCEEVESCNADDVQIFNKHVFTENGGERLKLLARSLEGFYSNPVSEQEIDSFVENPDHDERIKELLFGVDVVEGSLRCDTCGLIYPIRCSIVETVDTIESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7APQ2</accession>
    <name>I7APQ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">3-hydroxy-3-methylglutaryl-CoA synthase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_100380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. HMG-CoA synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265351.1">
        <property type="nucleotide sequence ID" value="XM_009267076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APQ2"/>
    <dbReference type="GeneID" id="20564468"/>
    <dbReference type="KEGG" id="ero:EROM_100380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100380"/>
    <dbReference type="HOGENOM" id="CLU_008065_0_1_1"/>
    <dbReference type="OrthoDB" id="1269963at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013746">
        <property type="entry name" value="HMG_CoA_synt_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08540">
        <property type="entry name" value="HMG_CoA_synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase N-terminal" evidence="3">
        <location>
            <begin position="7"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="4">
        <location>
            <begin position="184"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Hydroxymethylglutaryl-coenzyme A synthase C-terminal" evidence="4">
        <location>
            <begin position="252"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="382" mass="42816" checksum="254EF5880A569889" modified="2012-10-03" version="1">MPAKNMFGIIGLEYQLPSLYINQEEVANNIDFEVDKIKIGLGLEQMGVPSRREDAISLALNAVSRLITRYGVSPLDVGRLEVGTESNPDGSKSIKSYLTDLFPGNTSMCGCDNVHACYSGTNALLNAIAWMESSLWDGRFAIVVATDVAMYKDKNCLPTGGAGAVAILLGPNCIYRVIPSSITHYFSNQFDFMKPKELHPFPVVKGKESIENYRTAFEICYGELKKKMDDLGSFDYIALHSPYTRLPEKICVANHIDRKKFIKSLEASRKNGNAYSASLYLSLISLLDGHDGEIGEKVLLFSYGSGTSSSMFCLERVSKGCMITGFRERLSERKEISYQEFIKLIEDPIPQDNYETQEKVYLPGYYLKRISSFARHYEFLTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7APS4</accession>
    <name>I7APS4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_100630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265376.1">
        <property type="nucleotide sequence ID" value="XM_009267101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APS4"/>
    <dbReference type="GeneID" id="20564493"/>
    <dbReference type="KEGG" id="ero:EROM_100630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100630"/>
    <dbReference type="HOGENOM" id="CLU_327073_0_0_1"/>
    <dbReference type="OrthoDB" id="5752at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040240">
        <property type="entry name" value="TAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022591">
        <property type="entry name" value="TAF1_HAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12157">
        <property type="entry name" value="DUF3591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="6">
        <location>
            <begin position="775"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="879" mass="101634" checksum="812B778B7E29CDFF" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7APY9</accession>
    <name>I7APY9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_010320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263874.1">
        <property type="nucleotide sequence ID" value="XM_009265599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APY9"/>
    <dbReference type="GeneID" id="20520659"/>
    <dbReference type="KEGG" id="ero:EROM_010320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010320"/>
    <dbReference type="HOGENOM" id="CLU_2250137_0_0_1"/>
    <dbReference type="OrthoDB" id="4726at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF12">
        <property type="entry name" value="EUKARYOTIC INITIATION FACTOR 4B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="26"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="11783" checksum="DF660DB4A1D77483" modified="2012-10-03" version="1">MEGESDIERLQRMQQSYLDIQEKPVSSVYVKNLDASVTNSDIAAHFRSCGNISGINVITTKNTRKYAIVDFTSEKSVEMSLLFNGSVLKGKKIIVQRKREVDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ14</accession>
    <name>I7AQ14_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GOLD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263899.1">
        <property type="nucleotide sequence ID" value="XM_009265624.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ14"/>
    <dbReference type="GeneID" id="20520685"/>
    <dbReference type="KEGG" id="ero:EROM_010580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010580"/>
    <dbReference type="HOGENOM" id="CLU_099520_0_0_1"/>
    <dbReference type="OrthoDB" id="2193035at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25388" checksum="AFDCC0C78DEE0B72" modified="2012-10-03" version="1">MFFLFLGHVFSRTLFYLDKSKEVESALRLNGQTVVLMQADLFHKESASYPWKEAKGTSPGIGLSIYKSDGDYKNADPDPKKLTFKDVKRDMSTTFFYTTPETGYYTMVFSLDKDVQGEQALGLAIYEGRPWIPEIVSGTDYQMEWLIRKMSDLLYASKNNFDLQNLDDWDEVEYMILYNSIFKLINGVVLLKIGTVFVTLLYINKKTKEFYVSKKIIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ59</accession>
    <name>I7AQ59_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_110320</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. KIN-13 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265511.1">
        <property type="nucleotide sequence ID" value="XM_009267236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ59"/>
    <dbReference type="GeneID" id="20564631"/>
    <dbReference type="KEGG" id="ero:EROM_110320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110320"/>
    <dbReference type="HOGENOM" id="CLU_430888_0_0_1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01367">
        <property type="entry name" value="KISc_KIF2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000012">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971:SF8">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47971">
        <property type="entry name" value="KINESIN-RELATED PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50105">
        <property type="entry name" value="SAM_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 10" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAM" evidence="13">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="173"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="257"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN84014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="581" mass="66404" checksum="9EB356074CAC4783" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ87</accession>
    <name>I7AQ87_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263974.1">
        <property type="nucleotide sequence ID" value="XM_009265699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ87"/>
    <dbReference type="GeneID" id="20520760"/>
    <dbReference type="KEGG" id="ero:EROM_020020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020020"/>
    <dbReference type="HOGENOM" id="CLU_839451_0_0_1"/>
    <dbReference type="OrthoDB" id="1933455at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN TRANSFERASE MAEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="1">
        <location>
            <begin position="115"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="336" mass="39452" checksum="2391A27252AF8317" modified="2012-10-03" version="1">MQIVLMDLSTIQKARISSTLRTTCKLMERLFQMYRCRKISKEEYMEKLEKLKEVVGSNIDEMKRNTRIMASRNEPTNTLNVFIMEHFEHIGCFGTASALASKFSIEAYSDADFYKRVYGIKNQIENGLFEEALSFCREYRVELKSMKCEEAASLENDLKVEKFIEMCHGQEFDKALEFINKEFKRVPEQIKSYLPVLVSNSNFRKYPFSRHTKTAEQFLVCALKLFRRGLKSRLMQRIEYGMMAYKTYRCINTENNKCPTCCKAFRLREDVPFNKHEISILLCKGNSEEMDDTNQPYVFEDGFIYGAKYIELSEHICASSKSIDNALRYPRLCFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQC9</accession>
    <name>I7AQC9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HRDC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111020</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265581.1">
        <property type="nucleotide sequence ID" value="XM_009267306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQC9"/>
    <dbReference type="GeneID" id="20564701"/>
    <dbReference type="KEGG" id="ero:EROM_111020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111020"/>
    <dbReference type="HOGENOM" id="CLU_771674_0_0_1"/>
    <dbReference type="OrthoDB" id="2250022at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071044">
        <property type="term" value="P:histone mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071040">
        <property type="term" value="P:nuclear polyadenylation-dependent antisense transcript catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002121">
        <property type="entry name" value="HRDC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045092">
        <property type="entry name" value="Rrp6-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124:SF47">
        <property type="entry name" value="EXOSOME COMPONENT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12124">
        <property type="entry name" value="POLYMYOSITIS/SCLERODERMA AUTOANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00570">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50967">
        <property type="entry name" value="HRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HRDC" evidence="3">
        <location>
            <begin position="272"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="360" mass="41724" checksum="53B8ECABB6E75EC1" modified="2012-10-03" version="1">MNLLDMSIKFVEGINLFLRQESHDLNGLEIERLVQRIEEIQLLVKDSEDRFKRILESIEVEESIRPDTIVAITKNKKPLCILGKEIVKPRIESIDAENIDCCHTPSFGEFKAFSSTVYVKTLDQLLDMDKTITQAAIEMFDHSYRSYEGFTCFISVGDAKGNVYIIDAIKFRAAISRLELLKCHVPKIIHCRACVERLLRDFRQVGCFRSYEISPKMVFIDWRIRPVPGFLLEVLFKGVHEIREMAASGVEMKVYCSKEIDETKDMASRYGISEDEEILENLLKLRRFLARSNDESVHYVMTDDQVVRLLKAKPTTQEKMVEVLKRLSPLARQHIMDFILLFSGSKKSFLLKDLMEPNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQE9</accession>
    <name>I7AQE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">White ABC transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_111230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265601.1">
        <property type="nucleotide sequence ID" value="XM_009267326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQE9"/>
    <dbReference type="GeneID" id="20564722"/>
    <dbReference type="KEGG" id="ero:EROM_111230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111230"/>
    <dbReference type="HOGENOM" id="CLU_000604_1_10_1"/>
    <dbReference type="OrthoDB" id="2196280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF113">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY G MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="15"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="301" mass="33726" checksum="5E707CA17DA068D5" modified="2012-10-03" version="1">MPEHNLDLKRVVLEVPNQNLSSSEKYASLIKDLDANFESGKLYAFMGTSGSSKTTTMEAIAGVIPFGSLTSGEILMDNNERSDDTWEKMSTYERQKGYTIDELTVDEFVYYSTVFSLPNESKKNIEEAMDEVLGNLLGLGHVRSNRMEKLSGGERKRVGIAISFMKMLLLEGKLKVVLLDEPTSELDSGLAVKVVRFARDYARRNGSIVILTVHQPGPELYSTFDDLLFMHKGLRIYAGPSNDFMKYLNSKGIYNTGGPGTDTEFIFSLFTSGTKEAEQYKDQIKQIEMEAKNKKEVKGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQI3</accession>
    <name>I7AQI3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_021020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="HOGENOM" id="CLU_339802_0_0_1"/>
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        <property type="component" value="Chromosome II"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="835"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="835"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="EMBL" id="AFN82577.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="835" mass="96788" checksum="B6B79EBDF8534CCC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQZ5</accession>
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        </submittedName>
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    </gene>
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        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264249.1">
        <property type="nucleotide sequence ID" value="XM_009265974.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20521044"/>
    <dbReference type="KEGG" id="ero:EROM_031300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031300"/>
    <dbReference type="HOGENOM" id="CLU_031211_0_0_1"/>
    <dbReference type="OrthoDB" id="2191972at2759"/>
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        <property type="component" value="Chromosome III"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="624" mass="71081" checksum="5CFB06900C8A06D4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR18</accession>
    <name>I7AR18_ENCRO</name>
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        <submittedName>
            <fullName evidence="9">Glucose transporter type 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_040180</name>
    </gene>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="9 10">SJ-2008</strain>
        </source>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
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        <property type="nucleotide sequence ID" value="XM_009266009.1"/>
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    <dbReference type="OrthoDB" id="2196240at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF8">
        <property type="entry name" value="FACILITATED GLUCOSE TRANSPORTER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="163"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        </location>
    </feature>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="16"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="398" mass="43673" checksum="E825D4296AB3755A" modified="2012-10-03" version="1">MDARKTPEIRKSAYYSAAMASMSSVLFGISLTIMGCMHPYVSKMWRETMDQTEVDNLWGIVSSNIFLGCLASNILASIIRPNMKGALILNNILYTMGYITFFFSQSFGSIFSGRFIIGLASGITCAIVPIYTSLISPIECRGLLLSFHPLGITVGITLGNALSCFNTESTWKIPLIITLFLIGLSLIGLLGITDPFYQEKPSDISLYALIKNRRARRSVFLVMLVHVSQHLCGVDYITLFLRDLFPADMYSPKITAVLISLASVVITAFSGRYVDRIGRKPLIIISSLVAGGATAMFTFGIYPTAAALLFVAGYNMGLCSIPWFITAEIFPPKYASAAGLLGVSLNWVSAYTTLAALYPLHIKYGRIVFSFYTICMALFAGLMAVFFKETKNKVPAFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR36</accession>
    <name>I7AR36_ENCRO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264304.1">
        <property type="nucleotide sequence ID" value="XM_009266029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR36"/>
    <dbReference type="GeneID" id="20521102"/>
    <dbReference type="KEGG" id="ero:EROM_040380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040380"/>
    <dbReference type="HOGENOM" id="CLU_778514_0_0_1"/>
    <dbReference type="OrthoDB" id="2195292at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007720">
        <property type="entry name" value="PigQ/GPI1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05024">
        <property type="entry name" value="Gpi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="356" mass="41191" checksum="584C17E0FD20B443" modified="2012-10-03" version="1">MEIRHIVLLSYGNIDRINQQVMCDGCLVLNSPWSDSSPETKMKRNGDELTIESKEMVLKVINGKIVYIEAVYLIDSKVEFVELTLSPHPYDFFLLKKHFLSRLKGTPSNSTDKEVVYLRKRRIGRFVIPVFLYLLKTTIEYLFVGIPQHMVDYIVNCSTEEKRIITIKTTLGTYIKFFCSIQGAFLRLGFPIVFIDEIFVVPTTMYLSAFSYACSFLARSLIESIHLLNNSSYNPLKRRRDSVILNTDQIFMSALIFSFILLIMFNIVHFYILCSFMSLCLVGLKFITDFIDFVLMDISKCRTYTLSINYDRMPAVELRYTKVSVYDRIKLAVQASLLSLESFKDNILVKLLLGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR62</accession>
    <name>I7AR62_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vesicle transport V-snare protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264339.1">
        <property type="nucleotide sequence ID" value="XM_009266064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR62"/>
    <dbReference type="GeneID" id="20521138"/>
    <dbReference type="KEGG" id="ero:EROM_040740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040740"/>
    <dbReference type="HOGENOM" id="CLU_120587_0_0_1"/>
    <dbReference type="OrthoDB" id="2187995at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="189" mass="21414" checksum="32056ABB71BBE9C6" modified="2012-10-03" version="1">MLFFCVISLVVCSRIQKILRPGVKEVYWAILDNEDVELNIQLNQYSDMPVYYKVIKPGEVEEDSGIGEELVEGVDLRYTTPGAYKIEVYNDGSEKASISVYTDVMSSGDVDNDNLAIKNLFLDLEAKLMSLYRTNTRLKTIQENNIAEAKRIRNGLYIMFAIPIMYVIIGLAKLHTTKAMFAPKKGSRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR69</accession>
    <name>I7AR69_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">phenylalanine--tRNA ligase</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_040840</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264349.1">
        <property type="nucleotide sequence ID" value="XM_009266074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR69"/>
    <dbReference type="GeneID" id="20521148"/>
    <dbReference type="KEGG" id="ero:EROM_040840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040840"/>
    <dbReference type="HOGENOM" id="CLU_020279_2_0_1"/>
    <dbReference type="OrthoDB" id="1698572at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000002">
        <property type="entry name" value="phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="16">
        <location>
            <begin position="276"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AFN82852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="557" mass="62773" checksum="11DEFEF5ABE70B92" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS20</accession>
    <name>I7AS20_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264664.1">
        <property type="nucleotide sequence ID" value="XM_009266389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS20"/>
    <dbReference type="GeneID" id="20521470"/>
    <dbReference type="KEGG" id="ero:EROM_060740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060740"/>
    <dbReference type="HOGENOM" id="CLU_1077794_0_0_1"/>
    <dbReference type="OrthoDB" id="2194829at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="258" mass="28419" checksum="268767A9FBD50FE5" modified="2012-10-03" version="1">MEENESPEKPGRTKIPFELIISTVNFLIATAQVGLHLKIGTPSGLVMKVSRFTTSAVLLVPAFKILYQLHRFVRDHGVRKMNRVKILWMVAYLLALFSGIAFLFVKPNMTDLKGQAMIVSMASLLGMVISCALLDYKHLPKRNYIDYLIIFSCLVFIVMLASLLPPLKKHDMVSKVAAWGSMATGLIIPVLQSLLSPGSISEPVSYEDFAMFNVLSIAILALSIAAVASPHVLEWYDKKPRTNTARTLASINNGGTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS31</accession>
    <name>I7AS31_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">N-alpha-acetyltransferase 40</fullName>
            <ecNumber evidence="4">2.3.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_060950</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-seryl-[histone H2A] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H2A] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50600"/>
            <dbReference type="Rhea" id="RHEA-COMP:12742"/>
            <dbReference type="Rhea" id="RHEA-COMP:12744"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-terminal L-seryl-[histone H4] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-seryl-[histone H4] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50596"/>
            <dbReference type="Rhea" id="RHEA-COMP:12740"/>
            <dbReference type="Rhea" id="RHEA-COMP:12743"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64738"/>
            <dbReference type="ChEBI" id="CHEBI:83690"/>
            <dbReference type="EC" id="2.3.1.257"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. NAA40 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.257" evidence="4"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264684.1">
        <property type="nucleotide sequence ID" value="XM_009266409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS31"/>
    <dbReference type="GeneID" id="20521491"/>
    <dbReference type="KEGG" id="ero:EROM_060950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060950"/>
    <dbReference type="HOGENOM" id="CLU_1758790_0_0_1"/>
    <dbReference type="OrthoDB" id="424551at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043998">
        <property type="term" value="F:histone H2A acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039949">
        <property type="entry name" value="NAA40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20531:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="6"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="146" mass="17498" checksum="EFF55F48DC807810" modified="2012-10-03" version="1">MERRVLKIERLSKDLLKWAVNLVVSNLGRYCRSPLMKFSKAKSLKNKNNNYIFCFVGEVPVGFITFRFSRDTTYVFELHVEKEHRSLGIGALLLDECKKQYDKVVRRIVLYVYRENSRGLKFYERNGFRINEGYECSRFYEMILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS72</accession>
    <name>I7AS72_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acid phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histidine acid phosphatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264759.1">
        <property type="nucleotide sequence ID" value="XM_009266484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS72"/>
    <dbReference type="GeneID" id="20521569"/>
    <dbReference type="KEGG" id="ero:EROM_070110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070110"/>
    <dbReference type="HOGENOM" id="CLU_706151_0_0_1"/>
    <dbReference type="OrthoDB" id="10257284at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07061">
        <property type="entry name" value="HP_HAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033379">
        <property type="entry name" value="Acid_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000560">
        <property type="entry name" value="His_Pase_clade-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050645">
        <property type="entry name" value="Histidine_acid_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567:SF110">
        <property type="entry name" value="2-PHOSPHOXYLOSE PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11567">
        <property type="entry name" value="ACID PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00328">
        <property type="entry name" value="His_Phos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00616">
        <property type="entry name" value="HIS_ACID_PHOSPHAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="357"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="367"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="395" mass="45593" checksum="5ABB6DE5407B95A3" modified="2012-10-03" version="1">MIKILLRMSLLFDGKWNTRNISTEKDLEPFRRYCQSGYKFIPKAENYNLEKLFVIFRHGARTPTRNLTRTWDSQECMPCSLNNTTISDCKRKECSEGDLTHRGFMQMITLGKFIKNNYNSLLFDKKIEQKNIEMRATKIPRTHSSLAGVVRGLTGDTVVENVEIPKSDDTLLSTLGCTTQKEREDVMEFFNKPNIVQDNQVFSRHPKPQERADHYYTSLCSRVPVDCRELNCDIKSVEEHIKAANDAWAYMANIGNNTEEKRKFMFGRFARDLLLDIGKEKHIFLYSAHDSSIGAILTGFDTGISEWPSYASALFIEIWCNTGKQYVRMIFNNRVIKPKSFSDEYIPIRDFMELLKKTIPDPPNKNPDAKSTKNDAETEGKIEKKDEVEKKANSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS82</accession>
    <name>I7AS82_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger domain-containing ubiquitin ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_070260</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264774.1">
        <property type="nucleotide sequence ID" value="XM_009266499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS82"/>
    <dbReference type="GeneID" id="20521585"/>
    <dbReference type="KEGG" id="ero:EROM_070260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070260"/>
    <dbReference type="HOGENOM" id="CLU_912244_0_0_1"/>
    <dbReference type="OrthoDB" id="2193062at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="262"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="55"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="307" mass="34517" checksum="6BB16E0A51615BE9" modified="2012-10-03" version="1">MARRNGNNNFLNIMRSMLSFLDARIESSDADTNNSPGGVFMSRLRSRLVDLINGRMETRNNEGDERAASDTEGSSRSVGRRRRARKNLSGARQEAPTVEERSLVDRCSRMINETGERPVRGRRQVRAASVSQNRRVDGSRGEMSMDPVMLTTSIVVTGNERSSSQSSLQNEEGRSDVRSDEESRSLGNRGFRVVFPRVPSQELRFLFLTPQSEGARGNVIYEIQINFDIFDASTEPPVTATKESLKKTDVVKATKDDETCECTICMSNFSMNQKLRVLPCDHKFHTGCVDKWLLGHSNKCPVCRKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASA6</accession>
    <name>I7ASA6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Rad50-like DNA repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_070560</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264804.1">
        <property type="nucleotide sequence ID" value="XM_009266529.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASA6"/>
    <dbReference type="GeneID" id="20521615"/>
    <dbReference type="KEGG" id="ero:EROM_070560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070560"/>
    <dbReference type="HOGENOM" id="CLU_006184_1_0_1"/>
    <dbReference type="OrthoDB" id="18797at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003691">
        <property type="term" value="F:double-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051880">
        <property type="term" value="F:G-quadruplex DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867:SF12">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18867">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="11">
        <location>
            <begin position="6"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="458"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="787"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="892"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="961"/>
            <end position="1010"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1249" mass="146351" checksum="775D8815D50C7658" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASC2</accession>
    <name>I7ASC2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264824.1">
        <property type="nucleotide sequence ID" value="XM_009266549.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASC2"/>
    <dbReference type="GeneID" id="20521635"/>
    <dbReference type="KEGG" id="ero:EROM_070760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070760"/>
    <dbReference type="HOGENOM" id="CLU_075093_0_0_1"/>
    <dbReference type="OrthoDB" id="2190329at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="338" mass="38900" checksum="CAC6CAD379F4B32A" modified="2012-10-03" version="1">MILNRLSKHSKDIKKYQKLIKVALVIVPLLIFRVKKQSEISSAEPVQHYLDGTIKVVNRDTGPFFIGNKFALNSLPPRLSLRIQGYPEYKENYHPLISTLRSMNIRNINVIIDLNPPDEKCIYLFEIDLSFGSKACVLIHPLKNHQPNSDFLLVAGEFFPFKLAFDPMDIILTNTPSNRLYIVLKDTPRSLKVFLNFIKSISFIRTHPYGSYFYIPVNGGYIDMIVFVPLVLSVLAYYTFDWAYAPYHISTPRIFIGALLYSFFPMSFLFFTRKNESPCLAIIFMVLNPKIGLAYIGICYLRMVWDVISIIRSTFKSPDVSLINAELVLSRKAKSKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASH8</accession>
    <name>I7ASH8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264884.1">
        <property type="nucleotide sequence ID" value="XM_009266609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASH8"/>
    <dbReference type="GeneID" id="20521696"/>
    <dbReference type="KEGG" id="ero:EROM_071360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071360"/>
    <dbReference type="HOGENOM" id="CLU_440771_0_0_1"/>
    <dbReference type="OrthoDB" id="2191041at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="566"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="604" mass="69163" checksum="ACD82A60B32B23A2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASI2</accession>
    <name>I7ASI2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264889.1">
        <property type="nucleotide sequence ID" value="XM_009266614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASI2"/>
    <dbReference type="GeneID" id="20521702"/>
    <dbReference type="KEGG" id="ero:EROM_071410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071410"/>
    <dbReference type="HOGENOM" id="CLU_1137993_0_0_1"/>
    <dbReference type="OrthoDB" id="2192334at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="244" mass="28399" checksum="DC3D752C076A3E23" modified="2012-10-03" version="1">MLYDLVIVIVVLVFGFLLSQRKKRRLQKKALLLEPFKNHFEESNGEYLSIHQYILKLSGNPNLKYLCAIITLRRDFCLSYLFGPVPKENFILTGQLKARVPCVYVFRKSLPLRHYGLKYTKKCLLANIPGYKAFGPLEEKHLEFIKKYEVLTFFISYAPLNIEDPADFESLVFLKASLPLLNSTEFIDDFLALFDNVTLESGKKFLEMKQGYKKDIEVLKAKENRSLGEKLASRIREKSKTKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASM2</accession>
    <name>I7ASM2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cell division protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_080150</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264934.1">
        <property type="nucleotide sequence ID" value="XM_009266659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASM2"/>
    <dbReference type="GeneID" id="20521751"/>
    <dbReference type="KEGG" id="ero:EROM_080150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080150"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF46">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000654">
        <property type="entry name" value="Integrin-linked_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="12" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="265" mass="30444" checksum="373EC058EA8B352F" modified="2012-10-03" version="1">MTRYILGGVIGSGTYGEVYEAIDTETKEKVALKRIKLNEKEGMPGTALREISILKKLNHRNIISLVSTIHTDTLLTMVFPFIDYELKKYIEMNTGRNIMELINQLICGVHYLHRMNVVHRDLKPQNILVTSDGVLKIADFGLSRSLEIRVPPYSSEVVTLWYRSPELLMGSTSYRFYVDIWSLGCIIYEMITLEPLFPGESKENQLTLIRRKAGTRRSLKNVVEQSLAAPKFLIEIILRCLDLNYNQRITADEIIGILENEYEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASQ7</accession>
    <name>I7ASQ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264974.1">
        <property type="nucleotide sequence ID" value="XM_009266699.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASQ7"/>
    <dbReference type="GeneID" id="20521793"/>
    <dbReference type="KEGG" id="ero:EROM_080570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080570"/>
    <dbReference type="HOGENOM" id="CLU_457842_0_0_1"/>
    <dbReference type="OrthoDB" id="2191197at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="596" mass="70219" checksum="7BD9095EFBC1A009" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASS5</accession>
    <name>I7ASS5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264994.1">
        <property type="nucleotide sequence ID" value="XM_009266719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASS5"/>
    <dbReference type="GeneID" id="20521814"/>
    <dbReference type="KEGG" id="ero:EROM_080780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080780"/>
    <dbReference type="HOGENOM" id="CLU_630091_0_0_1"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="435" mass="48913" checksum="6B98F83B4BA4B1A4" modified="2012-10-03" version="1">MLLKSLEPFFGIPSAPNTIIYNTFWLAALTFLNIFYINEHHVKIARHITKKIKNIFIQALALCFLNSIPKIILMVNLYRTSSIQVFASAISGTTQISMAISLALVLILAKTNCFVHHISFYKDILFLEASHLLLLIMFTNTTDTLTAPLMALGVFSTFLFNTFSLPQYISQASNNDGQEIISSETQPPSTSLVLYPLRFVFNLVFLDPSHLFPSSSKKTPYTVALSPIINFLIGALYFRMRMGRFELLLSLMCSFLVGLLLYMMVRKRILSTISCLYSLAASILFFSIIMESFINLATHVSSATGLGIQFLSGTFLSLQCNLMEIVTCLNYNGGEMLVISSCSILYTHMYNILLFFPVTKLLLGKTGAYAQSHNISNAPFVYFSYVFILAEIKVIFVNYLLRHHKLTKDLGYTLIAIYVLFITGFAIEGKSHLCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AST1</accession>
    <name>I7AST1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_080840</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264999.1">
        <property type="nucleotide sequence ID" value="XM_009266724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AST1"/>
    <dbReference type="GeneID" id="20521819"/>
    <dbReference type="KEGG" id="ero:EROM_080840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080840"/>
    <dbReference type="HOGENOM" id="CLU_060400_1_0_1"/>
    <dbReference type="OrthoDB" id="1109245at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032277">
        <property type="entry name" value="Ribosomal_eS4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16121">
        <property type="entry name" value="40S_S4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS4 N-terminal" evidence="9">
        <location>
            <begin position="4"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 central region" evidence="8">
        <location>
            <begin position="95"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein eS4 C-terminal" evidence="10">
        <location>
            <begin position="210"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="268" mass="29671" checksum="DD3B43CD3F52B285" modified="2012-10-03" version="1">MPTGHRDHLKRKTAPESWMLDKQGGTFALMPASGPHRKTECIPLGYLISRFLKYAGNSKELGIVLGGKNIKVNGKVRTNSHFPVGLFDVISIEKTGEHFRVLYNTHKKFHLHKISSQEAGYRLAKVTKKYTSRGIPYVVSSCGLSIRFCDPAIEQEYTIKISNETSKVVEHIAPGVDKVVFVSRGKARGRIGVINTIDVQGKETVYVMTDFAGNTFSCTSKSCIIIGESSENIWITLPKENGIRLSELEKINAQLGEMVDTGAIEVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT13</accession>
    <name>I7AT13_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_081760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265089.1">
        <property type="nucleotide sequence ID" value="XM_009266814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT13"/>
    <dbReference type="GeneID" id="20521911"/>
    <dbReference type="KEGG" id="ero:EROM_081760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081760"/>
    <dbReference type="HOGENOM" id="CLU_129464_0_0_1"/>
    <dbReference type="OrthoDB" id="2436667at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13156">
        <property type="entry name" value="KOW_RPL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000915">
        <property type="entry name" value="60S_ribosomal_eL6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041997">
        <property type="entry name" value="Ribosomal_eL6_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10715:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01159">
        <property type="entry name" value="Ribosomal_L6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="19586" checksum="98DB196513895295" modified="2012-10-03" version="1">MIIKEVRVIPDDAGLYMPDDIPKFVEKYKARTARKPRVRRTDLVKGMVVVVLEGVFASKRVVYLKGLEDNLALCAGPKSINGVPLFKIDERYLLATSTVLDINVNVDIDEKDIILTKRDDYTAPMDVEMTDAEKKIDEEIAKVAKEIKFMKSYLSEPFEIDTTRNFYSLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGU5</accession>
    <name>I6ZGU5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription-associated recombination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263905.1">
        <property type="nucleotide sequence ID" value="XM_009265630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGU5"/>
    <dbReference type="GeneID" id="20520691"/>
    <dbReference type="KEGG" id="ero:EROM_010640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010640"/>
    <dbReference type="HOGENOM" id="CLU_816429_0_0_1"/>
    <dbReference type="OrthoDB" id="2190904at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045114">
        <property type="entry name" value="Csn12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732:SF0">
        <property type="entry name" value="PCI DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12732">
        <property type="entry name" value="UNCHARACTERIZED PROTEASOME COMPONENT REGION PCI-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="295"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="351" mass="40728" checksum="1C580BDEBF133D8F" modified="2012-10-03" version="1">MGYQCRIRISPMGWVEAINSRIDAEDGEYVSRLFSKNAEMDIEQGNIDLSGVKYPFNILLEHQKAAVEKKSYGAHELLIKSTVRMVGRERWTSPIIKRVVYELLEPWGRYNPNRMSKLLADIYKKLIEQKNPAFVYVGNALFHIHLETGRFKLAEDLLMIVKEPETVCRDYYVFYYYKGIIKMYQESFKESYLALERAFGYKKWRRTMAPVYFISSLLVNKFPKDIYLKRFGCDYLSEVVSVVRGGFYMEVDDAIRSASKGVVNHNLCRIISAHCPLICFNNLVNRTYLQYGCDSRLDIQKISEALPDIDFKEIVCLLSNAIESGRLRGYISISRRVVVFSKADPFPIIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH94</accession>
    <name>I6ZH94_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Small nuclear ribonucleoprotein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264065.1">
        <property type="nucleotide sequence ID" value="XM_009265790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH94"/>
    <dbReference type="GeneID" id="20520854"/>
    <dbReference type="KEGG" id="ero:EROM_020930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020930"/>
    <dbReference type="HOGENOM" id="CLU_2704787_0_0_1"/>
    <dbReference type="OrthoDB" id="429711at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="73" mass="8410" checksum="3641EC68395F8E28" modified="2012-10-03" version="1">MGGELKSILPLEKLYDFLSKSIDVEIWEKDIRVKHKGRIVGFDEYMNLVIDNGTKRYILKGDCICVIFGRDAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHD4</accession>
    <name>I6ZHD4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PRA1 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_021380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PRA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264110.1">
        <property type="nucleotide sequence ID" value="XM_009265835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHD4"/>
    <dbReference type="GeneID" id="20520900"/>
    <dbReference type="KEGG" id="ero:EROM_021380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021380"/>
    <dbReference type="HOGENOM" id="CLU_1686548_0_0_1"/>
    <dbReference type="OrthoDB" id="2194493at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004895">
        <property type="entry name" value="Prenylated_rab_accept_PRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19317:SF0">
        <property type="entry name" value="PRENYLATED RAB ACCEPTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03208">
        <property type="entry name" value="PRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="156" mass="17723" checksum="FD2DBA4C76702483" modified="2012-10-03" version="1">MQGGIQEEETFQKNIKEALGNKHVAKDFFNIRKASVPRDLNDAKRRILTNLDRFKFHYFAMASILTLIYVLYRLELIILIGIISITAYAYKTKPTLFNIEMEPRSVCIAGIVGILIFFIFFKEAIVGLLAISAICGIIILIHAASLEEDLQKDEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHI6</accession>
    <name>I6ZHI6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264125.1">
        <property type="nucleotide sequence ID" value="XM_009265850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHI6"/>
    <dbReference type="GeneID" id="20520916"/>
    <dbReference type="KEGG" id="ero:EROM_030090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030090"/>
    <dbReference type="HOGENOM" id="CLU_839632_0_0_1"/>
    <dbReference type="OrthoDB" id="2195577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="334" mass="37670" checksum="69B6B2E783A33084" modified="2012-10-03" version="1">MRKTYESASRLNKKMKSYRRRVLDPGTKLDVIKDDDNLENIDDYWKTAESVLRDDTTIEIEESSIYKSDTVEERNENDEPNTQSDTLFDIKVIRESLSAKSKEVQIPSCRESLETNSILENEGPPSSFESIEGENENCNENVMGGNGFSDGIGTSDFRIEEKYNTVSNDSRVLSRGVLEGKGISPAKEMRDRYRKRLQSLGKESERPVARKSMIQSKEIYEYKGETIYNSDGKEVAVTNISGLYRGRSAFEPLVTSSMLETAILFLNNLAFIKPEKAVCNFSIFMIKGTACIEMGQDKIILKRGSICVIEKETVYSISNSFGTGCTILLTYSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHL8</accession>
    <name>I6ZHL8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CHK1-like Ser/Thr protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264170.1">
        <property type="nucleotide sequence ID" value="XM_009265895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHL8"/>
    <dbReference type="GeneID" id="20520963"/>
    <dbReference type="KEGG" id="ero:EROM_030520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030520"/>
    <dbReference type="HOGENOM" id="CLU_1360388_0_0_1"/>
    <dbReference type="OrthoDB" id="539158at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="201" mass="22440" checksum="1F5674A46FA21F61" modified="2012-10-03" version="1">MSARDYRQDPFNRIRGQTMKLIMKMMAREGDRLSAAMIVKEPWVVQPNRFFDKSGLCGDPSGLFDLIPHESKVALHFTQPGEVHKASGTYFISSQTGGVRGGDVHRLYTKKGDGKSIVKEVCEALGVMVVPYKDVGESVAFSTIDSKRSVLSGEIGIKKLSENYCVIICRTKGDSREFEKFVKMLVDLTGYSSKEYLDIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHQ2</accession>
    <name>I6ZHQ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Recombinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_031010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264220.1">
        <property type="nucleotide sequence ID" value="XM_009265945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHQ2"/>
    <dbReference type="GeneID" id="20521014"/>
    <dbReference type="KEGG" id="ero:EROM_031010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031010"/>
    <dbReference type="HOGENOM" id="CLU_1354602_0_0_1"/>
    <dbReference type="OrthoDB" id="1861185at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033065">
        <property type="term" value="C:Rad51C-XRCC3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071140">
        <property type="term" value="P:resolution of mitotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090656">
        <property type="term" value="P:t-circle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46487">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46487:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="200" mass="22602" checksum="09F1FC9A7028510A" modified="2012-10-03" version="1">MLEYYGVTELTGKSGTGKTAIAVEESKELATIYITTTTFCIGRYKGASKEVMDRIIVKYIPSIEYLASFVMNSMERIIVERRTGLVIIDSLDHLLATEGMGMDRGILFGVVNRLKRMNQKYGVNILVVTYHYGSWTIGAFCIPNPMLGLQWMYMVNTRYVCSRAGDKRTLRLVRSPFMKSKAWEFVIGSSAVMITSEMPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHS7</accession>
    <name>I6ZHS7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rab GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_031310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264250.1">
        <property type="nucleotide sequence ID" value="XM_009265975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHS7"/>
    <dbReference type="GeneID" id="20521045"/>
    <dbReference type="KEGG" id="ero:EROM_031310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031310"/>
    <dbReference type="HOGENOM" id="CLU_041217_23_1_1"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000586">
        <property type="entry name" value="Rab family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="201" mass="22903" checksum="81751BBA32065C0E" modified="2012-10-03" version="1">MDSECKYLFKMILIGNSGVGKTCLMKRYTDETYSFTQSSTIGVDFKIKSLEVEGEKVKLQIWDTAGQERFRAIISNYYRGAHGIIMVFDMGSKESFDNLGDWLAEVRKNTNENVEIIILGNKVDDKEKIVVTEEEIERFLSENGIEKSSFYMTSAKDNICVNDSFEYLTRKLIDKHKRIGFGGGKESFKLNVGDSKKACCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHU1</accession>
    <name>I6ZHU1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dihydrofolate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_040010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264270.1">
        <property type="nucleotide sequence ID" value="XM_009265995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHU1"/>
    <dbReference type="GeneID" id="20521065"/>
    <dbReference type="KEGG" id="ero:EROM_040010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040010"/>
    <dbReference type="HOGENOM" id="CLU_2108994_0_0_1"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF0">
        <property type="entry name" value="DIHYDROFOLATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="115" mass="12419" checksum="469ED5B89CEFB0FC" modified="2012-10-03" version="1">MAGNQQRDYEKRKGMLENTAGSFPALSGFEKNVVIATTLFKDHGTQVGVIEAGVGHILDTTNVFSEESVITSVPTAISVDHSEILGETVEDIVEHRYALGENEGSCLWVPSLQKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIH8</accession>
    <name>I6ZIH8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ski2 RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_050970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264525.1">
        <property type="nucleotide sequence ID" value="XM_009266250.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521329"/>
    <dbReference type="KEGG" id="ero:EROM_050970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050970"/>
    <dbReference type="HOGENOM" id="CLU_004113_0_0_1"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001039">
        <property type="entry name" value="ATP-dependent RNA helicase DDX60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052431">
        <property type="entry name" value="SKI2_subfamily_helicases"/>
    </dbReference>
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        <property type="entry name" value="DEAD/H RNA HELICASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44533:SF4">
        <property type="entry name" value="DEAD_H RNA HELICASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="522"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="908"/>
            <end position="1085"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1335" mass="154336" checksum="CD871591E131CAC9" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIZ8</accession>
    <name>I6ZIZ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264670.1">
        <property type="nucleotide sequence ID" value="XM_009266395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIZ8"/>
    <dbReference type="GeneID" id="20521476"/>
    <dbReference type="KEGG" id="ero:EROM_060800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060800"/>
    <dbReference type="HOGENOM" id="CLU_042823_0_0_1"/>
    <dbReference type="OrthoDB" id="2192720at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="CDD" id="cd00060">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="238"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="364"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="411" mass="46685" checksum="6B7F53124D02FBB1" modified="2012-10-03" version="1">MGEKYKLSSLGSDDKVRSVAFTNESEITIGSGLDSDVRLQQPSIEERHVLVYFDHMKIRVLGNNVFLNEEALEKESMYGFRFGDVVKINRYKFVFSLTEPGDFVDEDKIIRRPTMELSYLNREGEEKKDLSEGGKRIKISNSGEEKGDGIGDAVVSIALNKVTGVNVSLCGEEVENVSSVKQVIEEQKEVLKKEIKESIETSMVDTPVLPSMGKKNSRDLGEVMIKKEILDSAEKVVEEKMKEEHAAIDEELDELKQNVKDNIKEELKEDLKRDFMEDVKEDIRDEVKEVLSSSDMKNAIATDVMSRIEGEKEEPDEEGDDKRREEKREKGDEEANERPGGRRRRSSAMRLLPQDDGEVGGVKTLRKASVRSKKRASEAPKETNKRRKGGKKEEEKTRDGETEKSNKDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ36</accession>
    <name>I6ZJ36_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061370</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264725.1">
        <property type="nucleotide sequence ID" value="XM_009266450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ36"/>
    <dbReference type="GeneID" id="20521534"/>
    <dbReference type="KEGG" id="ero:EROM_061370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061370"/>
    <dbReference type="HOGENOM" id="CLU_003612_2_0_1"/>
    <dbReference type="OrthoDB" id="2187858at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021846">
        <property type="entry name" value="NFACT-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11923">
        <property type="entry name" value="NFACT-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="6">
        <location>
            <begin position="421"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="NFACT protein C-terminal" evidence="7">
        <location>
            <begin position="567"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="271"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="648" mass="75484" checksum="A01F2013D5E9B9A1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJN1</accession>
    <name>I6ZJN1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hit-like hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_080310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264950.1">
        <property type="nucleotide sequence ID" value="XM_009266675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJN1"/>
    <dbReference type="GeneID" id="20521767"/>
    <dbReference type="KEGG" id="ero:EROM_080310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080310"/>
    <dbReference type="HOGENOM" id="CLU_056776_3_0_1"/>
    <dbReference type="OrthoDB" id="672793at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="5"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="15359" checksum="2D3AB3EC6B1B9E76" modified="2012-10-03" version="1">MEKCIFCTMYQKGNNIIYETAKAFVLMDRYPLSRGHFLVIPKAHHAYFHEYEPEELSDILGTINHLIQKFGIEKYNILQNNGNHQAVFHVHFHLIPFVSSSERLKINWETMSLSDEEYSEIVKEAKLKASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJS1</accession>
    <name>I6ZJS1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264995.1">
        <property type="nucleotide sequence ID" value="XM_009266720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJS1"/>
    <dbReference type="GeneID" id="20521815"/>
    <dbReference type="KEGG" id="ero:EROM_080800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080800"/>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_1"/>
    <dbReference type="OrthoDB" id="10259785at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000011">
        <property type="entry name" value="50S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="335" mass="37824" checksum="0E7C3914D688D11F" modified="2012-10-03" version="1">MKQQINCYEVDGVTVKKTLDLPEVFSTPVRQDLVLKTYSLYRMKTRQPYAVSKYAGMQHSAESWGTGRAIARVPRVKGGGTRRAGQGAFANFCRKGRMAHPTKTHRRWARKVVENTKKIAEAMAVAASAQTSLVEARGHRISDVKMLPIIVSDDLSKVKKTKDALQILKNLNMKEELKRVENSRGVRCGKGKMRNRRYTQRKGILIVHGESSELLAFRNIRGVDQMSIDSLDLLELAPGGMAGRLIIWTESAFEKLDKIFGGVEREAELTPGFKLPNKIVTHDDLEELFYSDAVQAILDEPLFVGRTELKLSEEEMKERLRFIKKFDASAIRSTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJT6</accession>
    <name>I6ZJT6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_081030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265015.1">
        <property type="nucleotide sequence ID" value="XM_009266740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJT6"/>
    <dbReference type="GeneID" id="20521837"/>
    <dbReference type="KEGG" id="ero:EROM_081030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081030"/>
    <dbReference type="HOGENOM" id="CLU_444824_0_0_1"/>
    <dbReference type="OrthoDB" id="2195102at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="614" mass="70489" checksum="E4045D6FD2BB9B8A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK14</accession>
    <name>I6ZK14_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Midasin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_081880</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the midasin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265100.1">
        <property type="nucleotide sequence ID" value="XM_009266825.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521923"/>
    <dbReference type="KEGG" id="ero:EROM_081880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081880"/>
    <dbReference type="HOGENOM" id="CLU_226725_0_0_1"/>
    <dbReference type="OrthoDB" id="5186at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000142">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041190">
        <property type="entry name" value="Midasin_AAA_lid_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17865">
        <property type="entry name" value="AAA_lid_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="307"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="655"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="947"/>
            <end position="1093"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="1225"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2412"/>
            <end position="2544"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1848"/>
            <end position="1875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2450"/>
            <end position="2467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2487"/>
            <end position="2502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="2519"/>
            <end position="2536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="2818" mass="325279" checksum="2672650FF6BC85CF" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKQ1</accession>
    <name>I6ZKQ1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Long chain fatty acid CoA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_100770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265390.1">
        <property type="nucleotide sequence ID" value="XM_009267115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKQ1"/>
    <dbReference type="GeneID" id="20564507"/>
    <dbReference type="KEGG" id="ero:EROM_100770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100770"/>
    <dbReference type="HOGENOM" id="CLU_000022_45_4_1"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="3">
        <location>
            <begin position="58"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="623" mass="70439" checksum="37B3C3A4ECD3D289" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL55</accession>
    <name>I6ZL55_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methionine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_110760</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265555.1">
        <property type="nucleotide sequence ID" value="XM_009267280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL55"/>
    <dbReference type="GeneID" id="20564675"/>
    <dbReference type="KEGG" id="ero:EROM_110760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110760"/>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_1"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methionyl/Leucyl tRNA synthetase" evidence="12">
        <location>
            <begin position="5"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="413"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN84058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="546" mass="63416" checksum="444D95E8F37CEE19" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL85</accession>
    <name>I6ZL85_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase</fullName>
            <ecNumber evidence="6">3.1.4.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_111060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + choline + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14445"/>
            <dbReference type="ChEBI" id="CHEBI:15354"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="3.1.4.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the phospholipase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.4" evidence="6"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265585.1">
        <property type="nucleotide sequence ID" value="XM_009267310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL85"/>
    <dbReference type="GeneID" id="20564705"/>
    <dbReference type="KEGG" id="ero:EROM_111060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111060"/>
    <dbReference type="HOGENOM" id="CLU_336498_0_0_1"/>
    <dbReference type="OrthoDB" id="14911at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004630">
        <property type="term" value="F:phospholipase D activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09141">
        <property type="entry name" value="PLDc_vPLD1_2_yPLD_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016555">
        <property type="entry name" value="PLipase_D_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896:SF76">
        <property type="entry name" value="PHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PHOSPHOLIPASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00614">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009376">
        <property type="entry name" value="Phospholipase_D_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="8">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="8">
        <location>
            <begin position="677"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR009376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="845" mass="96874" checksum="26382CD739006C3D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLA7</accession>
    <name>I6ZLA7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_111320</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265610.1">
        <property type="nucleotide sequence ID" value="XM_009267335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLA7"/>
    <dbReference type="GeneID" id="20564731"/>
    <dbReference type="KEGG" id="ero:EROM_111320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111320"/>
    <dbReference type="HOGENOM" id="CLU_066040_1_0_1"/>
    <dbReference type="OrthoDB" id="2516at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="111"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN84113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="251" mass="28410" checksum="692AC1A6D3B21298" modified="2012-10-03" version="1">MLYLVGFGLHSYKDITLRGLEAVKNSTKVYLESYTSIHGEPLDAFEELIGRKVYPADREMMEQTDRIVDESSEENVSLLVVGTPLFATTHSDIMIRAKEKGVKVEVIHNASIINVLGCCGLYSYSFGRVVSIPYFAGRWKPTSFYDNIVRNYQNNLHTLCLLDIKADENRFMSVNEAIDQILEAGKILNSDLVNENTRIFAICRFGSPTEEMVYGRIGDLRTRSFGDPLHSLIMPAKLDRVEEELVDSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS12</accession>
    <name>I6ZS12_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DnaJ-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_010360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263878.1">
        <property type="nucleotide sequence ID" value="XM_009265603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS12"/>
    <dbReference type="GeneID" id="20520663"/>
    <dbReference type="KEGG" id="ero:EROM_010360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010360"/>
    <dbReference type="HOGENOM" id="CLU_936989_0_0_1"/>
    <dbReference type="OrthoDB" id="2191416at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="11"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="271"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="297" mass="34854" checksum="50477629F15B2CEB" modified="2012-10-03" version="1">MGNRQSKIEKSPHEVLGLSPVSTKKEVKDCYRTLILKVHPDAQKVHSSKAPKEAMEIMEAYTSIMRSPPGFEFYTEELFRKDLRKYADDFFERISDYCRIPGAPKFSDPDFERFYQVFTNFRTQKVFDSEEEKSEFCRSVRKVARIVKSLDKRISIDSFSIEAHPAPPKVREKKTKLYPYNCDHCSKGFHSHNQIIDHFRSKKHFERIRLVSEDPKKYIEKQLQEITCQDLSEITQEPCTEKELEIVEDHKAEEQSVPKKTGHIREPAPFRTCAECKAVFNSRAELLTHLKMGHKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS15</accession>
    <name>I6ZS15_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CID domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263883.1">
        <property type="nucleotide sequence ID" value="XM_009265608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS15"/>
    <dbReference type="GeneID" id="20520668"/>
    <dbReference type="KEGG" id="ero:EROM_010410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010410"/>
    <dbReference type="HOGENOM" id="CLU_136889_0_0_1"/>
    <dbReference type="OrthoDB" id="10069473at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006569">
        <property type="entry name" value="CID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04818">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51391">
        <property type="entry name" value="CID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CID" evidence="1">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="166" mass="19188" checksum="E01035F4B00E2AF6" modified="2012-10-03" version="1">MASLSKEYLLKSLINLLPTEEQMKTVGLFIRTFKRDYRAIVEGWMFVYRKSSPYHRLNLLYLANEVVQTTKDVDPDSIELKILLKKVVNEVFEETKKMAMKNPSLYKKYCELENVWIQRNVMVLESHGINLGDLIRNIEKTFNDKAKLSAVLEDALSRVRKGSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSF2</accession>
    <name>I6ZSF2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HscB-like chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264033.1">
        <property type="nucleotide sequence ID" value="XM_009265758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSF2"/>
    <dbReference type="GeneID" id="20520821"/>
    <dbReference type="KEGG" id="ero:EROM_020610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020610"/>
    <dbReference type="HOGENOM" id="CLU_068529_3_0_1"/>
    <dbReference type="OrthoDB" id="445556at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021:SF15">
        <property type="entry name" value="IRON-SULFUR CLUSTER CO-CHAPERONE PROTEIN HSCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="146" mass="17312" checksum="E1A0F033C57E98CF" modified="2012-10-03" version="1">MGSPTRYFDIFGLKPSFSIDKHDLKERYFEISKRIHPDKARPSPGEGTSIEEINKAYDVLRNDLARARYLSSTKKFDVGKQFLMEILDYEQEISGVSNDEDKRNIREDLQKRIDHCKLHINEEHLAKWGYYERLMKMLNNKKKEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSK9</accession>
    <name>I6ZSK9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_021160</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264088.1">
        <property type="nucleotide sequence ID" value="XM_009265813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSK9"/>
    <dbReference type="GeneID" id="20520878"/>
    <dbReference type="KEGG" id="ero:EROM_021160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021160"/>
    <dbReference type="HOGENOM" id="CLU_088696_2_1_1"/>
    <dbReference type="OrthoDB" id="28335at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08048">
        <property type="entry name" value="HFD_TAF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000061">
        <property type="entry name" value="TFIID subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045127">
        <property type="entry name" value="TAF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006809">
        <property type="entry name" value="TAFII28_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13218">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04719">
        <property type="entry name" value="TAFII28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII28-like protein" evidence="8">
        <location>
            <begin position="47"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00072882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="148" mass="16875" checksum="1D464777A7A536D0" modified="2012-10-03" version="1">MSESNDEAEKEQNTKDLQSDTESDDLNLMTDDSTYKKEDPGRLHSITGAMSEEELHRYEKFRGSGFPKAAIKKYISSVIGQAVNPNFVIAVCGLAKVFVGEMIEIAKIVQEERQEEGPLLPSHIHEAYRRLYKRIPNTKIFKKAPWNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT48</accession>
    <name>I6ZT48_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">26S proteasome regulatory subunit 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_040270</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264293.1">
        <property type="nucleotide sequence ID" value="XM_009266018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT48"/>
    <dbReference type="GeneID" id="20521091"/>
    <dbReference type="KEGG" id="ero:EROM_040270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040270"/>
    <dbReference type="HOGENOM" id="CLU_019273_0_0_1"/>
    <dbReference type="OrthoDB" id="10252509at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041433">
        <property type="entry name" value="RPN1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040892">
        <property type="entry name" value="RPN1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18051">
        <property type="entry name" value="RPN1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17781">
        <property type="entry name" value="RPN1_RPN2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="RPN1 N-terminal" evidence="4">
        <location>
            <begin position="6"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="26S proteasome non-ATPase regulatory subunit RPN1 C-terminal" evidence="5">
        <location>
            <begin position="748"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="795" mass="89343" checksum="C7FC4FBCB1BAF56A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT76</accession>
    <name>I6ZT76_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_040630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264328.1">
        <property type="nucleotide sequence ID" value="XM_009266053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT76"/>
    <dbReference type="GeneID" id="20521127"/>
    <dbReference type="KEGG" id="ero:EROM_040630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040630"/>
    <dbReference type="HOGENOM" id="CLU_004962_8_1_1"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07415">
        <property type="entry name" value="MPP_PP2A_PP4_PP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="301" mass="34475" checksum="5AFAD3A7E1F4D08D" modified="2012-10-03" version="1">MIEGYLERLFKCNLLTEAEVRHLCDTATEMLMKEENVVNVKAPVTICGDVHGQFYDLMELFKVGGLPPYTNYLFMGDYVDRGYHSVETISILLCLKVKYPDRVWLLRGNHESRQITQVYGFYDECVRKYGTSLVWRYFTDLFDYLPVAAVVGNDTFCCHGGLSPSFDTLDQLKAIDRKVEIPHEGPMCDLLWSDPDEKLGWGPSPRGAGFTFGKDITDSFNERNGLKMICRAHQLVMNGYNWSHEKGCVTIFSAPNYCYRCGNLATLMEMDEYGTYSFTQFEPSPTKIETLVSTKIPDYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTC3</accession>
    <name>I6ZTC3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclear protein export factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264388.1">
        <property type="nucleotide sequence ID" value="XM_009266113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTC3"/>
    <dbReference type="GeneID" id="20521188"/>
    <dbReference type="KEGG" id="ero:EROM_041250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041250"/>
    <dbReference type="HOGENOM" id="CLU_036727_1_0_1"/>
    <dbReference type="OrthoDB" id="264795at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070390">
        <property type="term" value="C:transcription export complex 2"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045107">
        <property type="entry name" value="SAC3/GANP/THP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005062">
        <property type="entry name" value="SAC3/GANP/THP3_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436">
        <property type="entry name" value="80 KDA MCM3-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12436:SF3">
        <property type="entry name" value="GERMINAL-CENTER ASSOCIATED NUCLEAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03399">
        <property type="entry name" value="SAC3_GANP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC3/GANP/THP3 conserved" evidence="1">
        <location>
            <begin position="31"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="585" mass="68208" checksum="A66C345BCA0D1A1D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTE9</accession>
    <name>I6ZTE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264428.1">
        <property type="nucleotide sequence ID" value="XM_009266153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTE9"/>
    <dbReference type="GeneID" id="20521230"/>
    <dbReference type="KEGG" id="ero:EROM_041660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041660"/>
    <dbReference type="HOGENOM" id="CLU_723675_0_0_1"/>
    <dbReference type="OrthoDB" id="2189704at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="428" mass="49945" checksum="A6777186DE116A68" modified="2012-10-03" version="1">MRTETGGGSKKTFKTMKLGFLGLVGMVVAYYVLLFIFGIKYLDPQYYGSYFYAWRVNFADKLMTHGRYKLIKKNEHPDTEDRKRFYRISQDESGPYLIRFDRPEHFPRGHEHQFTLNFPGYEDFLKIRERFIVETEGLEEDMNLSHSDLMERMKKKKEGGGMFFADYRGKSISEMQKILFPNNNSEGNPWFTVSNILIKTISSIMSSDEEKRKGYLFDGEKIGDDLMKNIRDALEALDESAGDGDVLLSEKISDSDINKFFINNGKVSATPYETFAYSRAYYLFNFLTAGIEFKSQKLAELRSESSSDDNEFREARMDYTANLFARIFSSIYPNQHRKDVSSIGTLEKIRNYYSPKMVVDEKKEVDEADLELIQKSFSNPLRKECNGCCSPSQQQPLQGYWCYRVVYMCRDDEELIKFCYKYILLARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTG4</accession>
    <name>I6ZTG4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_050180</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264448.1">
        <property type="nucleotide sequence ID" value="XM_009266173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTG4"/>
    <dbReference type="GeneID" id="20521250"/>
    <dbReference type="KEGG" id="ero:EROM_050180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050180"/>
    <dbReference type="HOGENOM" id="CLU_020079_0_0_1"/>
    <dbReference type="OrthoDB" id="2195826at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19854:SF15">
        <property type="entry name" value="TRANSDUCIN BETA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="400"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="530"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="570"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="261"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="286"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="779" mass="88629" checksum="22B54A40135C8636" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTN6</accession>
    <name>I6ZTN6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Iron hydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_051000</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NARF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264528.1">
        <property type="nucleotide sequence ID" value="XM_009266253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTN6"/>
    <dbReference type="GeneID" id="20521332"/>
    <dbReference type="KEGG" id="ero:EROM_051000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051000"/>
    <dbReference type="HOGENOM" id="CLU_683513_0_0_1"/>
    <dbReference type="OrthoDB" id="10253113at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.950.10">
        <property type="entry name" value="Fe-only Hydrogenase (Larger Subunit), Chain L, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050340">
        <property type="entry name" value="Cytosolic_Fe-S_CAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009016">
        <property type="entry name" value="Fe_hydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004108">
        <property type="entry name" value="Fe_hydrogenase_lsu_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11615">
        <property type="entry name" value="NITRATE, FORMATE, IRON DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02906">
        <property type="entry name" value="Fe_hyd_lg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53920">
        <property type="entry name" value="Fe-only hydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iron hydrogenase large subunit C-terminal" evidence="3">
        <location>
            <begin position="78"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="357" mass="40778" checksum="81F974C2466325EB" modified="2012-10-03" version="1">MSFFTGLSGDNHKCIKTDTPFSLSLNDCLACSGCVSADEAHTLPVDTSFILDLSVRTSFILSPQSKINIFNIYRIGDMSYRKFESALSSFLKTKFNIYKIVDTSCMRSRIYEEVYREYLTTNHLVISACPGVVTYVERTAPHLIGYLSRVKSPQQMAFSLVKGSRTISVMPCYDKKLENGRDGVNFDFVLATREFRQVLDELGFEAFIKSRTMNLCDMEKTEISQWNIGTSSGGYSESILRRYCSIEVVKDRNGIKEYVVDDGSIISQITGLENSINYFRRSKIKGARYKMAEIFLCKSSCIGGPGQERVNDIEMDLKEYNLCGKEQPDIHYLPSELEEKRTFKQVKTRRVDFKVDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTV2</accession>
    <name>I6ZTV2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264598.1">
        <property type="nucleotide sequence ID" value="XM_009266323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTV2"/>
    <dbReference type="GeneID" id="20521403"/>
    <dbReference type="KEGG" id="ero:EROM_060060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060060"/>
    <dbReference type="HOGENOM" id="CLU_1594521_0_0_1"/>
    <dbReference type="OrthoDB" id="2192252at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="19634" checksum="C9EAF3718A3B420D" modified="2012-10-03" version="1">MKPEVGDVDEVMEQIARLAPPSQKEDHKFLNLLRKAIEKNTIVTPGPSLEKLAEKTEQRDFKVLSLALLRKDLPVKAREEFNEHIRRVKEKIEGLRKIGAQKEGVIDYEKRVERPNRIIEEINRIEESVPDVQKKIKKARDLIEEVFSKSSGRHLRPLEYAENFFKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUI7</accession>
    <name>I6ZUI7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">26S proteasome subunit alpha-4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_071000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264848.1">
        <property type="nucleotide sequence ID" value="XM_009266573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUI7"/>
    <dbReference type="GeneID" id="20521660"/>
    <dbReference type="KEGG" id="ero:EROM_071000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071000"/>
    <dbReference type="HOGENOM" id="CLU_035750_9_1_1"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="2">
        <location>
            <begin position="3"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="224" mass="25376" checksum="934BE3B34396E170" modified="2012-10-03" version="1">MGFEEQLAVFSPDGRLIQVEYAQQASEHGSLVVFGVDSEKISVSIERKSGNKLFLEEEKVFLLNEAQCIWMSYSGFKPDAYLVMNVARMMCLSYKISTGEDIAIDQLARKLSEYKQRYTVVYHQRPFGVRTVLFGLEPRPVAYVIEPDGNFSEFFCGAIGQKSQRVCEYLEKKEKEDLVKLTILGLMEVVQSDPQKISSFVLSKKGCERVEDPTIMGVINSVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUT2</accession>
    <name>I6ZUT2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chromosome condensation condensin complex subunit G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264973.1">
        <property type="nucleotide sequence ID" value="XM_009266698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUT2"/>
    <dbReference type="GeneID" id="20521792"/>
    <dbReference type="KEGG" id="ero:EROM_080560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080560"/>
    <dbReference type="HOGENOM" id="CLU_023055_0_0_1"/>
    <dbReference type="OrthoDB" id="27187at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027165">
        <property type="entry name" value="CND3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418:SF5">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14418">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="726" mass="83437" checksum="8A1BE10583937477" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUX7</accession>
    <name>I6ZUX7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_081350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265048.1">
        <property type="nucleotide sequence ID" value="XM_009266773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUX7"/>
    <dbReference type="GeneID" id="20521870"/>
    <dbReference type="KEGG" id="ero:EROM_081350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081350"/>
    <dbReference type="HOGENOM" id="CLU_660725_0_0_1"/>
    <dbReference type="OrthoDB" id="418911at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="295"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="409" mass="46999" checksum="2458F3666FA06C71" modified="2012-10-03" version="1">MGGVQIGGKIGRSMDGKKEEVGIGKPWNADPDLEAVVACCEDALKANPCNTEALYSLGKYHIENKNIDEAEIIATRLFELEPESYEGCVLSGYIGLQRIRLDRSYENFVKAYYKAEYKDKFLIYGISLFYEAIGDYTSARPWLVLLNKLGVEKYKSYETLFRTGVCLKKMNRLQDSINIFRVIVYDPLNNIYNPDVQIQMAHLYEMQSKHELAIEVLNGIEKPNRHHLIISRLYAWIDFKIGNFRGVKKWYRADSTTSNDPYLLYLVGRIRYMERNYMAALRKYMRTVQVDSIEGMVHNSIGCIYLRQGRFLLAKTAFMNALEINPKLSEASMNLKLIEEIVPKNKKETAEFMDSTLYNPSDPPPAIGKTRYLDSGEFFKDPAYNTYPGMPGVILPMKTSKFELLPGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVC9</accession>
    <name>I6ZVC9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265178.1">
        <property type="nucleotide sequence ID" value="XM_009266903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVC9"/>
    <dbReference type="GeneID" id="20564288"/>
    <dbReference type="KEGG" id="ero:EROM_090640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090640"/>
    <dbReference type="HOGENOM" id="CLU_072831_0_0_1"/>
    <dbReference type="OrthoDB" id="420884at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="333" mass="39083" checksum="FD4CDC8538CA1B96" modified="2012-10-03" version="1">MWGEKKFHSERNGEGKHLNYGEIERKRREKVDELAYRLLVEREKANQSIEEELQRLLDQENDSAETENISMEEAIEERHKDAPVTSTFSRSSEELQKTTNEIQSSCPTNVPFTEEFISRFILSKAYVLIEEKIWREKLIMPKTAQMQAKIQINKRMTQVSGKAGQVDKIFNVLKEYSHSYVFVETFTLKIIEQGRLQVSSCPGSYREFAELFCMFYSSELMEYFRVILFTREASVSTIKGIYSIYFGVLEIQNNADEAWFFIASVLNSRQNKLSCYVVECFFSILGGLLFRECRGPFFKLIEYVKRYYFKEMDNEPCKTRISSIFGMYGVQCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVM4</accession>
    <name>I6ZVM4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_091750</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265288.1">
        <property type="nucleotide sequence ID" value="XM_009267013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVM4"/>
    <dbReference type="GeneID" id="20564399"/>
    <dbReference type="KEGG" id="ero:EROM_091750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091750"/>
    <dbReference type="HOGENOM" id="CLU_030443_0_0_1"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046976">
        <property type="term" value="F:histone H3K27 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SET" evidence="3">
        <location>
            <begin position="368"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="502" mass="56904" checksum="69A7F2EF2563051F" modified="2012-10-03" version="1">MGSENGINKSLNKIVPIWMSRLYKDPALGYKKKMRRVMEKKENPSDLATKRHSDSSDFQPSVEKIVIPRIEPDIRSAFFTFTNISIKTEDDPVLRFVPCIPGAFGLINLAEFKEIGLLEKQWPCSKKTEEGAFYNSFDAVDGLRHNEKYSKGGELLEMRRLFCNVCFIFGCKWHSRTKGVCIKPVGFESNPSGSKSESKEVCGEVCKKMKRLHKSITKNDIDQSTYSVLYRDCWMSQGYGCIASLLFYLRHRKYIPCEEIKESCAALVGRHKYVIGNNNRGNIDPSAFFTPCDHPGDCVENNGCICASNRTNCEMSCLCVGCKNFFMGCMCPVACDDKCACRRAMRECIQICSCSLCGNKDLQMGNAEPTFVAPSGVEGYGLFAKEKISRGKFVIEYVGEIISNEEAERRGAFYDLRGCSYLFDLYNRGGIPLYVVDSRFIGNRSRFINHSKKNPNLNVSILLVNGIRRIGFYASRDIDKNEELFFDYGYSEEHKKKHGIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW25</accession>
    <name>I6ZW25_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">40S ribosomal protein S24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_101460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265458.1">
        <property type="nucleotide sequence ID" value="XM_009267183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW25"/>
    <dbReference type="GeneID" id="20564576"/>
    <dbReference type="KEGG" id="ero:EROM_101460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101460"/>
    <dbReference type="HOGENOM" id="CLU_107248_2_0_1"/>
    <dbReference type="OrthoDB" id="5571754at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="122" mass="14289" checksum="2DAAD2450F20BA4D" modified="2012-10-03" version="1">MRDNKLLSRRELDVVVYHPGMPIPSQEEIRGKISELYQTNKKNIVVGDFKNRYGTHRTTLKAKIYSSAEALSNTEKKHIVAKLTGKEFQITPRRVRKDERKKRYKVFGTLKKAMKKAEKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW30</accession>
    <name>I6ZW30_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Symplekin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265463.1">
        <property type="nucleotide sequence ID" value="XM_009267188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW30"/>
    <dbReference type="GeneID" id="20564581"/>
    <dbReference type="KEGG" id="ero:EROM_101510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101510"/>
    <dbReference type="HOGENOM" id="CLU_647279_0_0_1"/>
    <dbReference type="OrthoDB" id="2191847at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="423" mass="48334" checksum="79093CA5D1CD3786" modified="2012-10-03" version="1">MMSLELTRENINAVSLELVKNKRMFDKNVNYVMSFMIKAFPERTELLSRIKVLNIKSKAAKKIDLESDIMDQIAMNVFAIKNINEGSFMKNMLTLFKDPSSFSKVFLTYFYSNSSLGIEKTDEILDKLVELLLEESSLKESFEGFINGIGFSEYSESISSEKTEEMGKELGTDAHKELTYKASNDESSSTAISLISLDDSEEIRRIDTSISLLFEGRKKKLSPLSLKTCARVLSIMNIILENNYPGCVDVVPVLMYISKIDQVIFKRSLSALKTILKKINYKDKGRLFEMFCDLLGQNPNMIRMTMLIANTCGDYFSWPEFLESVDRNEEVDLDMIDRTCIPRPSFYEFIVLTENPGRYLTTAKNIIKNETDIHLLRDLGEKINDTELGGIKQIKSAIDSRIKALSHEECPKKQKRSGKNRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW34</accession>
    <name>I6ZW34_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spore wall protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_101560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265468.1">
        <property type="nucleotide sequence ID" value="XM_009267193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW34"/>
    <dbReference type="GeneID" id="20564586"/>
    <dbReference type="KEGG" id="ero:EROM_101560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101560"/>
    <dbReference type="HOGENOM" id="CLU_036413_0_0_1"/>
    <dbReference type="OrthoDB" id="2195471at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052258">
        <property type="entry name" value="Diverse_Func_Domain-Protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612">
        <property type="entry name" value="FIBROIN HEAVY CHAIN FIB-H LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37612:SF20">
        <property type="entry name" value="PER-HEXAMER REPEAT PROTEIN 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003706978" evidence="2">
        <location>
            <begin position="19"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="353"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="430" mass="44488" checksum="1C4FB0597D5B9400" modified="2012-10-03" version="1">MLKLSLLLSLVGFSAVLASGGRRKCGLRRNPDGFNYFKEQNLLGIKYKNDVRRLCGDQAFEDGSCNSSSYSSSSEECGRRCPKDRDDGCSSRSSSCDSYSSSCDYSSCSSTPCSTPVPLRCENELKTPIINMGERIFEFLKNYQDQYEKAVVLALTNILSQVSGFNPIFAGADYDALVEQLKTLGVNVPANTAAELASIDSSESAALSRTIQNNAQKVIGDLLGRISSMCYLDLVSLINSGLFASQISSVFNNIQPIITIAGNDLFAKQMAVFQKLSGSLPAAAVTAINNVLQANRNSFVTFFTTQASTLQTNVQDAVTALTTALTTLSNNASSEFVNFANSEIAALVSRVFPPSSTSDGSSGSGSSSGSGSSDGQGGSSGSGSSSGSGSSSGSGSSDGQGGSNGSGSNGGSGSSDGQGQGDQTPEQDGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW44</accession>
    <name>I6ZW44_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_101660</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265478.1">
        <property type="nucleotide sequence ID" value="XM_009267203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW44"/>
    <dbReference type="GeneID" id="20564596"/>
    <dbReference type="KEGG" id="ero:EROM_101660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101660"/>
    <dbReference type="HOGENOM" id="CLU_012062_4_3_1"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000025">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP19-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="44"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="215" mass="23891" checksum="C9978005C1C8A778" modified="2012-10-03" version="1">MGTNKKFINNLRFKPIMKNRNVLLLLIGVAINASSALGTPYMDLEYVVGGEKKSGRVTFELYWNEAPKTARNFYELVKGTEIGDGFYKYEGSLFHRIIPGFMMQGGDILMGNGTGSISIYDGKPFADEDFIKTHDSIGKLSMANSGPNTNGSQFFITFAPQPHLDRKHVVFGNVSEESIPLINDIQRVNTRHARPVIPVKIVKSGIIEEGGKEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWK1</accession>
    <name>I6ZWK1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265638.1">
        <property type="nucleotide sequence ID" value="XM_009267363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWK1"/>
    <dbReference type="GeneID" id="20564759"/>
    <dbReference type="KEGG" id="ero:EROM_111600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111600"/>
    <dbReference type="HOGENOM" id="CLU_121525_0_0_1"/>
    <dbReference type="OrthoDB" id="2192630at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="21832" checksum="B1CA8C93E203DF23" modified="2012-10-03" version="1">MHHKSTVKKTFKVVFFFIQALVFISGVGGSIVGTTVYLTAHELLQIPEKVLVISYLLSILEVLSAILGYTALVSRRKLRMLVYVLITLILMNTQAMMAVKGWTIYERSRAWGDWRWSSLTENQRSFVQTKFKCCGFSNPTDRSGPNCGGGKGCADVVYKLSRNVSVLMQRILMFLFFFESVGIGILSMLRLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACZ7</accession>
    <name>I7ACZ7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine hydroxymethyltransferase</fullName>
            <ecNumber evidence="9">2.1.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_011260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Interconversion of serine and glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:15481"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.1" evidence="9"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263967.1">
        <property type="nucleotide sequence ID" value="XM_009265692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACZ7"/>
    <dbReference type="SMR" id="I7ACZ7"/>
    <dbReference type="GeneID" id="20520753"/>
    <dbReference type="KEGG" id="ero:EROM_011260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011260"/>
    <dbReference type="HOGENOM" id="CLU_022477_0_1_1"/>
    <dbReference type="OrthoDB" id="10265628at2759"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049943">
        <property type="entry name" value="Ser_HO-MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000586">
        <property type="entry name" value="PRK00011.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680:SF35">
        <property type="entry name" value="SERINE HYDROXYMETHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 8 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Serine hydroxymethyltransferase-like" evidence="10">
        <location>
            <begin position="15"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="8">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000412-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="460" mass="50635" checksum="C8ED9402DC67F807" modified="2012-10-03" version="1">MESSKEKGFWTGSMETSDPELHALINGETERQKKTINLIASENYVHQSVMEANGSILTNKYSEGRVGERYYGGTHWIDRIEALCQKRALELFSLDPDVWGVNVQAYSGSPANFAVYTGLVPPGGKIMGLDLPSGGHLTHGYKTRTRKISATSVYFDSRSYKIGSDGLIDYSGLEESFMEFLPHLLICGYSAYSRDIDYKRLSMIANKNNAFLFGDISHISPLIASGLMESPFKYCDVVMTTTHKGLRGPRGALIFYRRSVRKGEEVVDLETKINFAVFPMLQGGPHNHTIAGIASMLLHAKSPSFVEYARRVVENSKTLCNHLLSLGYKVPTGGTDNHMFLVDLRGKGVDGSIVEHMCDALEISVNRNTVVGDTSPLNPSGIRVGTYAVTARGFGADEMKEVGDIINSIVCLCREVSMGKRMSKAEVEKVTSSEEFAGNSLAVDIKRRISSLVDAHPIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD27</accession>
    <name>I7AD27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264002.1">
        <property type="nucleotide sequence ID" value="XM_009265727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD27"/>
    <dbReference type="GeneID" id="20520788"/>
    <dbReference type="KEGG" id="ero:EROM_020300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020300"/>
    <dbReference type="HOGENOM" id="CLU_088000_0_0_1"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="240" mass="28233" checksum="5EFFA0F95E839FCC" modified="2012-10-03" version="1">MDIKELIVRVLENRDIGMINWEVYIEGDRVDLHELSTADILKYTFPYIKGVVNDKAGNKYDGLKLFKEYCEDCEYLNLVGEETVALCRGGGRDNKIKLWRKTKEFRYDFIDWEDREWVMKLIEYFYIMSMKNIEHLNREIMLLQNKSDKNPRGNEGMECIRVDSRGRVENVLVGRNDPTMTLDEFAEKIMAEMDKDSSLPPQDETSSTSSDDDPSREKLLKRDDERDEREISRGNTTRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD84</accession>
    <name>I7AD84_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thioredoxin reductase</fullName>
            <ecNumber evidence="7">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_020850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="8">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264057.1">
        <property type="nucleotide sequence ID" value="XM_009265782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD84"/>
    <dbReference type="GeneID" id="20520846"/>
    <dbReference type="KEGG" id="ero:EROM_020850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020850"/>
    <dbReference type="HOGENOM" id="CLU_031864_5_1_1"/>
    <dbReference type="OrthoDB" id="371245at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0274">FAD</keyword>
    <keyword evidence="2 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="9">
        <location>
            <begin position="3"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="309" mass="33184" checksum="9C4879B567703ABD" modified="2012-10-03" version="1">MEKVVIIGSGPAAYNAALYTMSRSPLLFEGGYIGNNGPGGQLTTTTAVDNYPGFPDGIEGPVLTSLMKDHAVSQGLRVAKETISDIRKRDGVFVVVSEKGEYRAEAVIVATGASARRLFVPGTGDDEFWQKGVSSCAVCDGFIYMNKITCVIGGGDAAMEEALYLSGIAKKVYIIHRRNTFRARNDMVKKAKAAENIEIMTPYVLERASGTKKVEEISIRNVETGEVKIIPMNGVFFGIGHDPNTSFLKNINVDLDPNGYVVVKDEACTSIPGLFAAGDVCDKRYRQAVTAAASGAISGMKAMEFLDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADD2</accession>
    <name>I7ADD2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="2">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_021300</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="2"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264102.1">
        <property type="nucleotide sequence ID" value="XM_009265827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADD2"/>
    <dbReference type="GeneID" id="20520892"/>
    <dbReference type="KEGG" id="ero:EROM_021300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021300"/>
    <dbReference type="HOGENOM" id="CLU_044879_0_0_1"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="3">
        <location>
            <begin position="228"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="486" mass="55740" checksum="C43920595B22F4FD" modified="2012-10-03" version="1">MSKGQLDLTQLVKSAVDFLSHLSSSRNMKSRYSYKKIAQELNRIEEPLYSLDGLKSIKGAGSKTIKRIQEFVDKKVVESIKTEDDLEKYSNILSPDAYDETKKKLSSRDMLGYSLGNDADEEKRIRIPRKRKYVPGYRTGSYGIMKALWIREGITKHEIAHIGRNYCDSEFDFTARHSAWSSMKTLTKKGIVYKEGRSKFYLTDEGRELATTMFANTSAIEEEEEEVTLIIDAREIKSRKFRLFFQEYFESKKIRHDTRILEVGDFLWIRGEKVCGFIIERKKGSDFVSSIMDGRFKEQKNRLKSTGIKKIFYIVEGLKSTHMQSVGKGLVMSCLATTKLEGFIVIETKDITQTGSVIHMIDCEVRKGYEKGLEAPDRLKKDEECEEYLKPCESSDGEMEMSYGSFIDKGAKGKGRTQTYLLYISLLSIKGMGHKKAWALAEFYKTIGELIGKVKNNGPGKLYEFEVEGKKISRKNADDIIEFFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADU7</accession>
    <name>I7ADU7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_031430</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264262.1">
        <property type="nucleotide sequence ID" value="XM_009265987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADU7"/>
    <dbReference type="GeneID" id="20521057"/>
    <dbReference type="KEGG" id="ero:EROM_031430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031430"/>
    <dbReference type="HOGENOM" id="CLU_055016_0_0_1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040176">
        <property type="entry name" value="RNF121/RNF175"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407:SF0">
        <property type="entry name" value="FI05221P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13407">
        <property type="entry name" value="RNF121 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="238"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="329" mass="38053" checksum="7F29E6676E117090" modified="2012-10-03" version="1">MKEEGKNENSSHGTGGKEEKVFDQAKVDKDDGDSFFDDIEAASYERYREGKEYRPIRQVIIIEREPTLGDVLQRIAVISVYALVIQTFFSAWKKINKKTHDICVFLILLLFPPGFFLYIQSYFLPFCWLLFSSFMLLNTLKVVKGSFGKDVMRDIYGVFKTLFIISNLGTFLGQSLTFMFFYLNPEYLGYTLSILLFFLYFGLLSREVIFFLSETMAISTGFYSKEGVPGKGNNNSLCMICTRSFDGSDRIHTLVCTHSFHEDCIKGWCLLGKKPFCPYCKKKIESSSLPPELWHKTEVWFYPLINTLRSFIVLTLVLTAIILYKIKYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADV4</accession>
    <name>I7ADV4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP cyclohydrolase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_040040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264272.1">
        <property type="nucleotide sequence ID" value="XM_009265997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADV4"/>
    <dbReference type="GeneID" id="20521068"/>
    <dbReference type="KEGG" id="ero:EROM_040040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040040"/>
    <dbReference type="HOGENOM" id="CLU_1855259_0_0_1"/>
    <dbReference type="OrthoDB" id="4966at2759"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP cyclohydrolase I" evidence="2">
        <location>
            <begin position="45"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="138" mass="15735" checksum="1E2B894B207D8834" modified="2012-10-03" version="1">MQAFSSLSRTKSPCQKRKKLDDRMGLKGLWCLHGGSWDRLQECLHTVDTPLRVSRLWVSDIAASFYAPPPRITTFDNTRGYGDLLFRGPIPIKSICSHHHSPFVGEAYIAYIPQREGRTVGLSNLNRIAKFYSRMPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADX0</accession>
    <name>I7ADX0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein tyrosine phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_040260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264292.1">
        <property type="nucleotide sequence ID" value="XM_009266017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADX0"/>
    <dbReference type="GeneID" id="20521090"/>
    <dbReference type="KEGG" id="ero:EROM_040260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040260"/>
    <dbReference type="HOGENOM" id="CLU_1115529_0_0_1"/>
    <dbReference type="OrthoDB" id="6058203at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050348">
        <property type="entry name" value="Protein-Tyr_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19134:SF449">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="2">
        <location>
            <begin position="24"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="3">
        <location>
            <begin position="169"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="245" mass="28595" checksum="9C08F97BFD4C201E" modified="2012-10-03" version="1">MTVNKELRDILNGYYDKIRSKIGENKLDEVIYLVDSLSATFKLSSSSYDRSSCTPYNNEVYEKYGVKEYVNASFVPTPRTLFIAAQNPKDEKRDVFLDFLMKSNTNLVVSLINDPKYFEEEWLQNRKVIKHVGKDLFIDEAYNVQGRSIRRLRYINWVDFSVITREEMETFHSYFDSIRSDPVVVHCVAGVGRTGTFIMYDILKGMKDITLDAFVNVFLSLRSKRAHLVTNKVQLEFLKNIFLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE09</accession>
    <name>I7AE09_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264347.1">
        <property type="nucleotide sequence ID" value="XM_009266072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE09"/>
    <dbReference type="GeneID" id="20521146"/>
    <dbReference type="KEGG" id="ero:EROM_040820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040820"/>
    <dbReference type="HOGENOM" id="CLU_122013_0_0_1"/>
    <dbReference type="OrthoDB" id="2194045at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="187" mass="22177" checksum="CA7378A8E7B62666" modified="2012-10-03" version="1">MMNGWRVFKYSRRMEKEGDEKSFCGVDLETMSQVILHSQPDSQGIKKERWLHVFTEGSKTLELILSPSMLEVRLYVVSAVRQELVILERIKLDNKWDIKTCVNKRALRYTCRERRFQIVFFSEEEVEVFGVKIGCFLRPHLDIEKTPVIERLTKAIEDGKKVRKTSKYKDKEKLIEEFLLLLFETPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEE1</accession>
    <name>I7AEE1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264527.1">
        <property type="nucleotide sequence ID" value="XM_009266252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEE1"/>
    <dbReference type="GeneID" id="20521331"/>
    <dbReference type="KEGG" id="ero:EROM_050990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050990"/>
    <dbReference type="HOGENOM" id="CLU_1111391_0_0_1"/>
    <dbReference type="OrthoDB" id="2191982at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="250" mass="28065" checksum="DB1E021892776D35" modified="2012-10-03" version="1">MTIVYEAVGMKRCARTGADFDRDLRRFSMLEKAVISMAYLLPIIIPSVAKSIDASYNLFLPLSMGLSASSFMPISARIARRFFDQDTVRIVTLHSSMVTIYVAIVFIYSLRMSAICYSNGFLESDISDGEMMYRMLILASYLFYDSGRLINRSVGLYRTFVVMSHLIGIFLISLLAIAYETGFASHSLLMQVFFCHSISSMVVSLAFDNFPSIITLKKDLDILPVVVSVGNLLTVILGVEIIFPRYNLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEH0</accession>
    <name>I7AEH0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA repair protein Mre11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264562.1">
        <property type="nucleotide sequence ID" value="XM_009266287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEH0"/>
    <dbReference type="GeneID" id="20521366"/>
    <dbReference type="KEGG" id="ero:EROM_051350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051350"/>
    <dbReference type="HOGENOM" id="CLU_1090001_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="255" mass="29301" checksum="3A7D04C008BA5C7D" modified="2012-10-03" version="1">MLIQKNSNAKETPPTRRCQAASICESYLHEEKSYIPMIRLKVELHGNDMFDRHRFSTQFKGLVANPNDMLSISRKTGRREEIEVHQPDEEKVEITHILRKILGNVEFGVLNRLCFSESLEEYVRGDSNAFMGMIRKNIEKVIDTIDHSSIVDEDVIKAMKKISREEDRSYDAEHVRTEENMGVSDGKEIETSSMDKKLEGGSLCHSSDSNEGSESRIKKNFALEDLIGRKYLGKRVRKNKEDSSTDVSFTFSKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF37</accession>
    <name>I7AF37_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264787.1">
        <property type="nucleotide sequence ID" value="XM_009266512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF37"/>
    <dbReference type="GeneID" id="20521598"/>
    <dbReference type="KEGG" id="ero:EROM_070390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070390"/>
    <dbReference type="HOGENOM" id="CLU_015083_0_0_1"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003864">
        <property type="entry name" value="CSC1/OSCA1-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02714">
        <property type="entry name" value="RSN1_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="415"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="528"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="573"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="599"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="3">
        <location>
            <begin position="53"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="3">
        <location>
            <begin position="262"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like 7TM region" evidence="2">
        <location>
            <begin position="319"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="728" mass="84335" checksum="1101E4F184010DBE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF45</accession>
    <name>I7AF45_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_070490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264797.1">
        <property type="nucleotide sequence ID" value="XM_009266522.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF45"/>
    <dbReference type="GeneID" id="20521608"/>
    <dbReference type="KEGG" id="ero:EROM_070490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070490"/>
    <dbReference type="HOGENOM" id="CLU_037460_0_0_1"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17868">
        <property type="entry name" value="GPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="252" mass="28809" checksum="03CBB10A7DCB34C5" modified="2012-10-03" version="1">MGYAIFVFGPAGSGKSTFCRNIQEHGESIGRSYRVINLDPAQISTSDNYALDLRDFITVGDVMEEYGYGPNGGLLVALEELYENISELELEDLEGSFLIFDCPGQIELFVHSEIMPKIIDYVSRYFRCGIVYVMESQYLVDINKYINGCFCALISMARMNVPCINVISKMDLIKNEDLDMFYTPNEDLSALIGTGRYSGLCKRMLSFVAENNMLNFYPLDWNKEEYIEELIYCIDNAVQYYEDAEPKTKDFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFA5</accession>
    <name>I7AFA5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hsp90 ATPase activator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264872.1">
        <property type="nucleotide sequence ID" value="XM_009266597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFA5"/>
    <dbReference type="GeneID" id="20521684"/>
    <dbReference type="KEGG" id="ero:EROM_071240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071240"/>
    <dbReference type="HOGENOM" id="CLU_1086229_0_0_1"/>
    <dbReference type="OrthoDB" id="567237at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="256" mass="29851" checksum="0980DFD398EFBD7E" modified="2012-10-03" version="1">MPEKRSNYHWAESDLSGWAKDKIKEALEARGYKVYGMDVLVKICQRMNTLGLVYMISFECTKDEKYCGVRNFYSVSEKADGMEDFEWFPKFFKEMEGEAVLKFGNRVLDVNKEMGERKPCAKSVSAEEAKTMDVSYKATINCEVRELMDFILSKEYISVWSGHRAAFEGDDILIDNVIIRELKEKNGGIEMKWKLKEWSNFTEVRIQLEEFLNSSKITLKQKNVPIKEEGSIRMWWNERVFVPISACFGFVLKPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFC3</accession>
    <name>I7AFC3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable threonine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Threonyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_071490</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264897.1">
        <property type="nucleotide sequence ID" value="XM_009266622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFC3"/>
    <dbReference type="GeneID" id="20521713"/>
    <dbReference type="KEGG" id="ero:EROM_071490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071490"/>
    <dbReference type="HOGENOM" id="CLU_008554_0_1_1"/>
    <dbReference type="OrthoDB" id="5423599at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01667">
        <property type="entry name" value="TGS_ThrRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000019">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000005">
        <property type="entry name" value="Threonyl-tRNA synthetase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
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        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
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        <location>
            <begin position="271"/>
            <end position="529"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="646" mass="74724" checksum="1AD3D78E9A9CB0B0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFS0</accession>
    <name>I7AFS0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265087.1">
        <property type="nucleotide sequence ID" value="XM_009266812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFS0"/>
    <dbReference type="GeneID" id="20521909"/>
    <dbReference type="KEGG" id="ero:EROM_081740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081740"/>
    <dbReference type="HOGENOM" id="CLU_567445_0_0_1"/>
    <dbReference type="OrthoDB" id="2190266at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="481" mass="55773" checksum="818B3F9CB22CF839" modified="2012-10-03" version="1">MRDEYSYNEGVESRSTEMLLSILEHYDTNLIEEHIIDEYDRRRKDSGRENEDMNLNKPLNAKSPEKHDSFHEKVDESEPLESHMCDFINSYGISEDDSCPQEKNKLGSLSLDKDGNDFEASSTNLTGNSTVDLSGVRNLTDPMSLEKALEFERWSIKKEELVRNTETQRKMLEAFHSIPEVALKEGIFDDIKINKIYVESRIHIRKENGRKIKEKKGLIRGERDIEGRKETKTMMFDDYASGWELSSESASDVATREKAFKIIREAKKKATGRDLLCSGDMQLRKFVVDQEEKYLKIEEVIKLNDLKYKNLMGKIYKKGGNGKYQYNWFTLKKNFFTCYNGKGIKIGSDTFPSDKNYDFKDPEDETLFFKKKYTIDLLSAGIYLVKHPAYMGCIRWGSSSDEDLIDITEDTKIIRITPAKNYFLISLKKNLVKTEIKTSTLDFALRADGETYFYRSKDMDSFLGWLVAFAFRQGRIKCNIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG60</accession>
    <name>I7AG60_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PDEase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091190</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265232.1">
        <property type="nucleotide sequence ID" value="XM_009266957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG60"/>
    <dbReference type="GeneID" id="20564343"/>
    <dbReference type="KEGG" id="ero:EROM_091190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091190"/>
    <dbReference type="HOGENOM" id="CLU_824126_0_0_1"/>
    <dbReference type="OrthoDB" id="295473at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004114">
        <property type="term" value="F:3',5'-cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="4">
        <location>
            <begin position="93"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="346"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="356" mass="41590" checksum="BDB475EEDA57E1C9" modified="2012-10-03" version="1">MDQSEEVPKDMCDVIARAVESKKNFCSKPVGDLRLSMSSYKFFHLEGISLADGIDAVMGTLVRDSRFNLLGVNPEALRLFFSFSFLHVLPQRYHNLDHLINTLCFGDFILRRLENVSCLPILDKMCLVIALSLHDVGHPGERNRKKIARLSKMYGNPEAMSFEMIHAAICRKGIVRYRDELFANLDDAELERRMDFIKRMIFATDLRLNKEIIDVFEDKYFEKRRGDLCRKVRNGIKGEISEIELKMIVKLADLSSCYKDYRIFNKNSIAFWSEVYDDDDYNRTLRDIYEDISLMEKISIPLADSFSLVFEDFRFLYNQALINLEEHKKYYKEVEREHSTKRSSAGEEDGFEANTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGH1</accession>
    <name>I7AGH1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nonsense-mediated mRNA decay protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_100490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265362.1">
        <property type="nucleotide sequence ID" value="XM_009267087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGH1"/>
    <dbReference type="GeneID" id="20564479"/>
    <dbReference type="KEGG" id="ero:EROM_100490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100490"/>
    <dbReference type="HOGENOM" id="CLU_013781_0_0_1"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="23"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="940" mass="108049" checksum="FB3854CF2F903CF6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGK2</accession>
    <name>I7AGK2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="7">AK6</shortName>
            <ecNumber evidence="7">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="7">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_100840</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="7"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265397.1">
        <property type="nucleotide sequence ID" value="XM_009267122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGK2"/>
    <dbReference type="GeneID" id="20564514"/>
    <dbReference type="KEGG" id="ero:EROM_100840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100840"/>
    <dbReference type="HOGENOM" id="CLU_079096_1_0_1"/>
    <dbReference type="OrthoDB" id="10251185at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="NMPbind" evidence="7">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="164" mass="18969" checksum="D8014FA27AA60D9A" modified="2012-10-03" version="1">MKILVTGTPGVGKTTFSSFISKKFKIPHIEISRYIEENNLYEEYSEVYKSLLFDDKTVKESLKKHVLDKESYIIDTHSCGMVERTAFDLVFLLTAPIEVLYKRLKERGYDEDKIKENVECEIFGVVREEVEDLFEEGYYVVGEEGLTFEEAVAIIGKNMDGRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGQ1</accession>
    <name>I7AGQ1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265457.1">
        <property type="nucleotide sequence ID" value="XM_009267182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGQ1"/>
    <dbReference type="GeneID" id="20564575"/>
    <dbReference type="KEGG" id="ero:EROM_101450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101450"/>
    <dbReference type="HOGENOM" id="CLU_2427028_0_0_1"/>
    <dbReference type="OrthoDB" id="2190770at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="80" mass="9476" checksum="7C9BD3098D27608A" modified="2012-10-03" version="1">MAHFGTNNLFNILDEIQNQILREEKPNTRKKALEDELHKLYLHTSLEICKQRLRGSVNKDTLSKVGKIKQYFKHIENIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGQ9</accession>
    <name>I7AGQ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265467.1">
        <property type="nucleotide sequence ID" value="XM_009267192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGQ9"/>
    <dbReference type="GeneID" id="20564585"/>
    <dbReference type="KEGG" id="ero:EROM_101550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101550"/>
    <dbReference type="HOGENOM" id="CLU_429620_0_0_1"/>
    <dbReference type="OrthoDB" id="2192235at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="644" mass="75178" checksum="B86EC4175AC38B2A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL53</accession>
    <name>I7AL53_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263876.1">
        <property type="nucleotide sequence ID" value="XM_009265601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL53"/>
    <dbReference type="GeneID" id="20520661"/>
    <dbReference type="KEGG" id="ero:EROM_010340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010340"/>
    <dbReference type="HOGENOM" id="CLU_980388_0_0_1"/>
    <dbReference type="OrthoDB" id="2191268at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="284" mass="33031" checksum="C9DF6CC597C1A44A" modified="2012-10-03" version="1">MKELVDVLYYDYILYKLANLYTLETASKEIGQYQKIAQKLLVKLRSDRVIFDAEIGLKFAYIKLYDLHLSFPKCTGRHHASCCRAHYLQFLSTSYDYLTKIMAEYSSLKECTAFSHLIYIVVVVRFVSSPTLEQFRDLNKEEDPESMKPYLAQQGIKAETEEEFANEKERLIKTLKIKAKDESSLFNKTFQYVMDELKRMFLSFDVPLLSFFGTSEVIQLKELYPNAFEGFEGVPGSSKSYKEEILKEYLDMRSILNKTRSAFINPKDKGEKILWGSDVSPSDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALC4</accession>
    <name>I7ALC4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">H/ACA ribonucleoprotein complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_011200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ("psi") residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the small nucleolar ribonucleoprotein particles containing H/ACA-type snoRNAs (H/ACA snoRNPs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263961.1">
        <property type="nucleotide sequence ID" value="XM_009265686.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALC4"/>
    <dbReference type="GeneID" id="20520747"/>
    <dbReference type="KEGG" id="ero:EROM_011200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011200"/>
    <dbReference type="HOGENOM" id="CLU_122030_0_0_1"/>
    <dbReference type="OrthoDB" id="2187159at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0698">rRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="101"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20701" checksum="5D691F460ABFB09A" modified="2012-10-03" version="1">MAKNFKNKKVQKSTETMELGKVLYMCQGQPVVKLMAKDIPYPNSPVLDLSSRVIGKVDEILGRLDDVHATIRLENPSGTLKRGETLLSYVDKFIPKKRFLPREEVEKKKEETDRKKSKFRKDQRAERGKPGAFKEKQRLHGRKTNNKWASRQNGKKQEFGKDNRRRKWDSSRSNRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALW7</accession>
    <name>I7ALW7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin ligase complex SCF subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_030200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264136.1">
        <property type="nucleotide sequence ID" value="XM_009265861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALW7"/>
    <dbReference type="GeneID" id="20520927"/>
    <dbReference type="KEGG" id="ero:EROM_030200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030200"/>
    <dbReference type="HOGENOM" id="CLU_059252_4_2_1"/>
    <dbReference type="OrthoDB" id="2342932at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="102"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="152" mass="18014" checksum="D12DD98A750770BA" modified="2012-10-03" version="1">MIEIETTDGRIFKIEEKQAYKSVLIKNVCTSTTYKNPIALQVSSFIFEIIQRYMEVDTSQLSKDYNPLEIRFKQSDLELFMEYDSRTLLDICNGANYLEYPYLLELCCKIISEKMKHKNTRELAEFIGVECNITDEELMKIEREFEWISSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM15</accession>
    <name>I7AM15_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030830</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264201.1">
        <property type="nucleotide sequence ID" value="XM_009265926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM15"/>
    <dbReference type="GeneID" id="20520994"/>
    <dbReference type="KEGG" id="ero:EROM_030830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030830"/>
    <dbReference type="HOGENOM" id="CLU_055156_0_0_1"/>
    <dbReference type="OrthoDB" id="28644at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1390.20:FF:000004">
        <property type="entry name" value="60S ribosomal protein L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="83"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="28186" checksum="C2F3966CF9D8D327" modified="2012-10-03" version="1">MERVMSEAPQSSIRRKEYEAKMSGIRRRQEKEFMERKKSNAKYAEEVTEKLLDRYYQQEREVLEKKASMEGGFYVPPEAEFFVVILIRSKCKCSPKVRKVLELFRLKRINNCVFVRNNKSTKKMLHIIKDYVAFGTIDMELLRELMYTKGTGRNGRTRVKLTNEFIEDMFDGKIKCIEELVHHIYNGTEMFKDVNNFLYPFHLSPPRGGFRCQKSKSFNDGGSVGNHQDLLGNLLRRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM32</accession>
    <name>I7AM32_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Phosphatidylinositol N-acetylglucosaminyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_031120</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264231.1">
        <property type="nucleotide sequence ID" value="XM_009265956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM32"/>
    <dbReference type="GeneID" id="20521025"/>
    <dbReference type="KEGG" id="ero:EROM_031120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031120"/>
    <dbReference type="HOGENOM" id="CLU_980389_0_0_1"/>
    <dbReference type="OrthoDB" id="196709at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="280" mass="32858" checksum="F087F661F27BE2E7" modified="2012-10-03" version="1">MSWKRCLYLQQEYPINYMEVRRVYRRRDVNEIYEDINRMVIRISIVMLFLSLFKVMMLEDKPGMSKSSWFSLFLHSTQNFLENREDKGINLKPGFMLLTFAYLLSPLIRTLTLEIHPNTLYIYFAITQILFIIDSVSASIYNTRSSMEVKDEQTPLSLEESINIPKRISSNQILGLTSYFLGFILLSSRFNEPSSVFNLLCLTFMGYIILLNHIEHLEVHRSTAATALMYVFVSMMTLLPDIRIFCIYSCIVTFVYSVSHISVWFLEKSLNRKVGYTNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMM1</accession>
    <name>I7AMM1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cleavage and polyadenylation specificity factor 100 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264466.1">
        <property type="nucleotide sequence ID" value="XM_009266191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMM1"/>
    <dbReference type="GeneID" id="20521268"/>
    <dbReference type="KEGG" id="ero:EROM_050360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050360"/>
    <dbReference type="HOGENOM" id="CLU_428282_0_0_1"/>
    <dbReference type="OrthoDB" id="64353at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027075">
        <property type="entry name" value="CPSF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45922:SF1">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="229"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="639" mass="72627" checksum="6704E611AA23D472" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMN3</accession>
    <name>I7AMN3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bromodomain associated domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264481.1">
        <property type="nucleotide sequence ID" value="XM_009266206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMN3"/>
    <dbReference type="GeneID" id="20521283"/>
    <dbReference type="KEGG" id="ero:EROM_050510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050510"/>
    <dbReference type="HOGENOM" id="CLU_1200153_0_0_1"/>
    <dbReference type="OrthoDB" id="2189721at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00076">
        <property type="entry name" value="HFD_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031498">
        <property type="entry name" value="Bromo_TP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17027">
        <property type="entry name" value="Bromo_TP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="26913" checksum="1C6ABB8C7B6128DE" modified="2012-10-03" version="1">MKSNMNTKILKLAVVEVLSQSGFDKTADQALNVLTDVLRYYIEQLGCRMKRRHGKGVVPEMMCRILVEETYGECEYQVPELLSFLRYQVTVKNYLSDRYNVGSEESILHILRVLPKNAQLRMVMRSGGNLREMNEVEREVVEDDVKFDEFTKEFVKSSLLEAGEREVREYRFESADLIGGEPQKRVRVGDIEFNEILDRKQKEVEFLQEPNTLVRDFGGWNSRYVFKGYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMP8</accession>
    <name>I7AMP8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ulp1 protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_050770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264506.1">
        <property type="nucleotide sequence ID" value="XM_009266231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMP8"/>
    <dbReference type="GeneID" id="20521309"/>
    <dbReference type="KEGG" id="ero:EROM_050770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050770"/>
    <dbReference type="HOGENOM" id="CLU_024324_2_2_1"/>
    <dbReference type="OrthoDB" id="1939479at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="51"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="279" mass="32733" checksum="87B1179AAB20CD6C" modified="2012-10-03" version="1">MDEEAVRSEDHHTTDDERMDVTGVVPSNQEQPDAIAEVCLRTSTPIKREGYELFPEDIERTKDGFMLNDKIINVYFELLAKHSKVGVYVFSTFFYTTLSKRGIPWVQRWTSGINIFENRLVYIPVYIPGHWMLIVFDVKKKVLEHYDSMGNAYTEVVHRILRYIRDEWSRVHKSEPSLSVDIKRKIPLQRNGRDCGVFVCMFGRYRLCGNEVWLSSDRIPRFRKLMLHEIVSGKILYNVFHKLVQPSSGLREHKGEVTDKKLLALETYEEVTKEKGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMU2</accession>
    <name>I7AMU2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA repair protein Mre11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264561.1">
        <property type="nucleotide sequence ID" value="XM_009266286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMU2"/>
    <dbReference type="GeneID" id="20521365"/>
    <dbReference type="KEGG" id="ero:EROM_051340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051340"/>
    <dbReference type="HOGENOM" id="CLU_009535_0_0_1"/>
    <dbReference type="OrthoDB" id="30417at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097552">
        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="340" mass="39459" checksum="E2F21CA6088DCC7B" modified="2012-10-03" version="1">MRILITSDNHLGYKESDPVLLDDSYETFEEILKVAQREKVDLILQGGDLFHDNRPSRNCLNRAIGLLRSYCVGSERSRLRSNRTLNSHDQNITISIPVVAIHGNHDDPSGINMVSPLDILQSSGLVNYIGKYSLMERIDVFPLLLEKEYRVAIYGLGHIKDRRLYRMFCEGKVVFHKPEDYDSWYNILVLHQNRVPREKEHLSLDFIDDFFDLVIYGHEHESRVTKEERLILQPGSTVRTSLCEGERHDKYVYILRIEEKCILEHVKLRSVRPLVLGVLRIEGKCNAEEIVAGKIKEMIEEGKKKETLFCKETTTVNVDTKKFKCERDTSNKTLPSSFYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANH6</accession>
    <name>I7ANH6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DnaJ-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264821.1">
        <property type="nucleotide sequence ID" value="XM_009266546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANH6"/>
    <dbReference type="GeneID" id="20521632"/>
    <dbReference type="KEGG" id="ero:EROM_070730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070730"/>
    <dbReference type="HOGENOM" id="CLU_119137_0_0_1"/>
    <dbReference type="OrthoDB" id="442087at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056453">
        <property type="entry name" value="HTH_DNAJC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052594">
        <property type="entry name" value="J_domain-containing_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44144:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23302">
        <property type="entry name" value="HTH_DNAJC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="23416" checksum="9FE589028BACB302" modified="2012-10-03" version="1">MKRRKDPYKILGVERTSTDMEVKRAYRRLQRAHHPDSKTGNRERYEEICRAYEEIQKRPTVEIIPVDDVMKVYKGSKEEVDDIIALYNRYKGKMAKIVDNLLLSDDRDESRLREIIDGLIECGALKKYNKYCKKVCEDAMRKKRKAREEKMARKIADEMGIDIDAPLEELLGRRREGEVSFLSRLEEKYLKGPKEEGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANQ4</accession>
    <name>I7ANQ4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VPS9 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264911.1">
        <property type="nucleotide sequence ID" value="XM_009266636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANQ4"/>
    <dbReference type="GeneID" id="20521727"/>
    <dbReference type="KEGG" id="ero:EROM_071630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071630"/>
    <dbReference type="HOGENOM" id="CLU_095016_0_0_1"/>
    <dbReference type="OrthoDB" id="300289at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.80">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003123">
        <property type="entry name" value="VPS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045046">
        <property type="entry name" value="Vps9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037191">
        <property type="entry name" value="VPS9_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101:SF25">
        <property type="entry name" value="GTPASE-ACTIVATING PROTEIN AND VPS9 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23101">
        <property type="entry name" value="RAB GDP/GTP EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02204">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00167">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109993">
        <property type="entry name" value="VPS9 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51205">
        <property type="entry name" value="VPS9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VPS9" evidence="1">
        <location>
            <begin position="79"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="257" mass="30060" checksum="74390FFA026BFAAE" modified="2012-10-03" version="1">MKCRKGCSKALLSLSLTPAGRTIDNFTRDIKLVKDNPTLLQTFYDYISIKYGVKCEVGISLVERMVMSDSFKQLMDTNDPTNELVNKKILLYRWINPEHLELRSVKRNILEDISKVLKSLPFLQTPTEKISCVMDVMERLCNSIGRNEGQDKILPSIIYCIIKSSVPDIYLETRFMVLYRRKNGERCKEKCNHELNIKADCECFFNKTYDEKEVSYYLTSVQAAVDFIRKMEFYDLKISEGEFYQNIMETIELVRDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANX3</accession>
    <name>I7ANX3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264991.1">
        <property type="nucleotide sequence ID" value="XM_009266716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANX3"/>
    <dbReference type="GeneID" id="20521811"/>
    <dbReference type="KEGG" id="ero:EROM_080750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080750"/>
    <dbReference type="HOGENOM" id="CLU_1094270_0_0_1"/>
    <dbReference type="OrthoDB" id="397265at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31222" checksum="B7E8DA42B71EBFD2" modified="2012-10-03" version="1">MKWDVDKEDDVNEPREEVKRVKKAIEGMDVKAVRPGKVSLNAEENGVRGQDLNFRRQVKRRVNDYKYKAFYNIGKTKRPYVFTRNSVVMMIEEELGVEILVKGSYKPEELNGDTDDEDALVYEISAETPEILQEAMAGLPSAINAIPPFPWNSSSIIGRYIYRNGARHRLHRVIIDNELAEKKINEIRKDIETTSVDKSVEISIRGRFSGYIEPCFGEESNGPTYIQIVAKTKREIKEAKNMCVKIIDKHREDFRNDCSTSLQTDESRKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP05</accession>
    <name>I7AP05_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP-dependent DNA helicase CHL1</fullName>
            <ecNumber evidence="15">5.6.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">ATP-dependent DNA helicase chl1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Chromosome loss protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16 17">DNA 5'-3' helicase CHL1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">EROM_081130</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">ATP-dependent DNA helicase important for chromosome transmission and normal cell cycle progression in G(2)/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="15"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265026.1">
        <property type="nucleotide sequence ID" value="XM_009266751.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP05"/>
    <dbReference type="GeneID" id="20521848"/>
    <dbReference type="KEGG" id="ero:EROM_081130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081130"/>
    <dbReference type="HOGENOM" id="CLU_006515_2_1_1"/>
    <dbReference type="OrthoDB" id="267079at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034085">
        <property type="term" value="P:establishment of sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF41">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE DDX11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="20">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00045008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00045702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AFN83529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="617" mass="70080" checksum="DADA081DE38FBEEA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP98</accession>
    <name>I7AP98_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265131.1">
        <property type="nucleotide sequence ID" value="XM_009266856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP98"/>
    <dbReference type="GeneID" id="20521955"/>
    <dbReference type="KEGG" id="ero:EROM_090160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090160"/>
    <dbReference type="HOGENOM" id="CLU_557800_0_0_1"/>
    <dbReference type="OrthoDB" id="447103at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051177">
        <property type="entry name" value="CIK-Related_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12984">
        <property type="entry name" value="SCY1-RELATED S/T PROTEIN KINASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="459"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="489" mass="56898" checksum="392BFB648C62038B" modified="2012-10-03" version="1">MFLKKLLAMATKNETLVHTTSTYTMYDTTFENKNATKLVFTQTLEAYSFLKKIKHDTIIGIYKLNVEKKYLVTRRLFPFYSVFKKEAKEFNKYISLKIAQAIMFLHDQCNIVHRNVVADSLFFNEEGGIVLGGFEKSRKSNTFDEDGIMFSNLVSELVGTNTNLLDFVKNGGFCSDIFFDLEIAFFGYKSFTTEQKLDFVSKMRREKNEFVDIHKRRISWMVLGDISSDAPKDFKILAVDFILCFDVPDMEEFLPPLFSILDTGIRFYLLRNSEKYIGKVSSLDPVIKSLSLGIKCKDKQLREETIVFIKENIPMISQRQQSEILETMYGYVSDEQGIQSVLRFLHDVKPVFKKSDVVYRILCKYLIQSKSKVQVLCTMEIFYETFDNFKMTTELLPLLCGYLSNRHAQPAVFSLIEKILVHLKEHKTEIIDKEWRIGSIKNIFKPKSSLQQEVIYGEKGEAQPAKIQSESDKECKEHKDPNDEWDDPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7APB1</accession>
    <name>I7APB1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265151.1">
        <property type="nucleotide sequence ID" value="XM_009266876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APB1"/>
    <dbReference type="GeneID" id="20521975"/>
    <dbReference type="KEGG" id="ero:EROM_090370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090370"/>
    <dbReference type="HOGENOM" id="CLU_174970_0_0_1"/>
    <dbReference type="OrthoDB" id="2193073at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="102" mass="11428" checksum="0099BCBB5D72D7E9" modified="2012-10-03" version="1">MGYKNKLLLACKKGEANGEKLSKKKAPDIHSMSDIVSLINLSNEERKAKLVSKYVEIISEFILYKARKMSGEKEKISTNDIKNVIVSEGLLFLSDIIGLNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I7APR4</accession>
    <name>I7APR4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Histone acetyltransferase</fullName>
            <ecNumber evidence="20">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">EROM_100530</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(2E)-butenoyl-CoA + L-lysyl-[protein] = N(6)-(2E)-butenoyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57332"/>
            <dbReference type="ChEBI" id="CHEBI:137954"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:53909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2-hydroxyisobutanoyl-CoA + L-lysyl-[protein] = N(6)-(2-hydroxyisobutanoyl)-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24180"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:15921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:131780"/>
            <dbReference type="ChEBI" id="CHEBI:144968"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:24181"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-lysyl-[histone] + acetyl-CoA = N(6)-acetyl-L-lysyl-[histone] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21992"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:21993"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:45949"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="20"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265366.1">
        <property type="nucleotide sequence ID" value="XM_009267091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APR4"/>
    <dbReference type="GeneID" id="20564483"/>
    <dbReference type="KEGG" id="ero:EROM_100530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100530"/>
    <dbReference type="HOGENOM" id="CLU_011815_2_2_1"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106226">
        <property type="term" value="F:peptide 2-hydroxyisobutyryltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140064">
        <property type="term" value="F:peptide crotonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000526">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000002">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025995">
        <property type="entry name" value="Tudor-knot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11717">
        <property type="entry name" value="Tudor-knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="22">
        <location>
            <begin position="106"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="71"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="19">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="AFN83869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="372" mass="43600" checksum="5DF5513D66AF96BA" modified="2012-10-03" version="1">MRLEDVYVGFKVPVKKIVNGTEEVFKAEILGRRTNKSGLEFYVHYVELNRRLDEWVDGSMVDLDDPSQIEVPKKKKAEKSRKTSEKEDEVKEEKPTLEKPPQDEEFKMKNVKKIMLRNHLVDAWYFSPYPKAIAESDVVYICEFCLYYFSTMDNLVLHAKQCKLRHPPGREIYRDGNLSFFECDGHIQKNYCRNLSLLSKLFLDHKSLYYDIDVFMFYVLCRLEDNGYQIVGYFSKEKMSEQGYNLACILTLPFEQRKGYGKILIDFSYLLSKRENMISGPEKPLSDLGLLSYRTYWMEVIVEYLSKHSKTSIADISKETHISEDDIIGTLSAYKMLRIDGNEFIFTYDEKQLQKALRSRSKCVDGSLLKLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ38</accession>
    <name>I7AQ38_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EB1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_010830</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263924.1">
        <property type="nucleotide sequence ID" value="XM_009265649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ38"/>
    <dbReference type="GeneID" id="20520710"/>
    <dbReference type="KEGG" id="ero:EROM_010830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010830"/>
    <dbReference type="HOGENOM" id="CLU_077755_0_0_1"/>
    <dbReference type="OrthoDB" id="2119228at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004953">
        <property type="entry name" value="EB1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036133">
        <property type="entry name" value="EB1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027328">
        <property type="entry name" value="MAPRE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10623">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN RP/EB FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03271">
        <property type="entry name" value="EB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140612">
        <property type="entry name" value="EB1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51230">
        <property type="entry name" value="EB1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EB1 C-terminal" evidence="8">
        <location>
            <begin position="171"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="134"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="238" mass="27660" checksum="04AB51B12EA95C91" modified="2012-10-03" version="1">MAQKSRRELIEWIRSLGIEISAIEELGKGVVICRILSLIHTDFPSNYVRNPDGEHDYLRNMKACQGFFASRNIKLYFPVDKLVKCKMQDNLEVAQWLAKYYSKNMGERKPRESTRDESLGTSASSSVNSHCSGCKNIKEIVDAKDNRIRELESTLSRMEDRITQMETEKRTIEEQATISGPKGFEDEEVKSLLIAFEKERDFYFKKLFTVEKYFLEKKGLDESLKNDVLSILYEENNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ48</accession>
    <name>I7AQ48_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome B5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010930</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263934.1">
        <property type="nucleotide sequence ID" value="XM_009265659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ48"/>
    <dbReference type="GeneID" id="20520720"/>
    <dbReference type="KEGG" id="ero:EROM_010930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010930"/>
    <dbReference type="HOGENOM" id="CLU_102602_4_1_1"/>
    <dbReference type="OrthoDB" id="260519at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004128">
        <property type="term" value="F:cytochrome-b5 reductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.120.10:FF:000007">
        <property type="entry name" value="Sulfite oxidase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051872">
        <property type="entry name" value="Cytochrome_b5/Flavoprotein_Rdt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237:SF1">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46237">
        <property type="entry name" value="CYTOCHROME B5 REDUCTASE 4 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00363">
        <property type="entry name" value="CYTOCHROMEB5"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="5">
        <location>
            <begin position="16"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="93" mass="10491" checksum="84E98B41318E1020" modified="2012-10-03" version="1">MNLIRWSRERSRASSLKALTAEEVSRHNKADDCWIIMDGMVYDVTDFLNLHPGGMDVIMKYGGKDCTDAFNEAHGYVNKELLLNSVVGIVTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ84</accession>
    <name>I7AQ84_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265536.1">
        <property type="nucleotide sequence ID" value="XM_009267261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ84"/>
    <dbReference type="GeneID" id="20564656"/>
    <dbReference type="KEGG" id="ero:EROM_110570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110570"/>
    <dbReference type="HOGENOM" id="CLU_2026715_0_0_1"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="122" mass="13658" checksum="433475B4DF32DF3C" modified="2012-10-03" version="1">MGAGRALMVLFNEGGKVLYKAVAKTISDIMVGDRMSKMEAEMILDISPSTSEDKVRESFLRMYYANAKENGGSPYIQSKILGAYTVLSGTSPRLLNLFEVDRRARQEDERKAPCANNKRFQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQB9</accession>
    <name>I7AQB9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dipeptide/tripeptide permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_110920</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265571.1">
        <property type="nucleotide sequence ID" value="XM_009267296.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQB9"/>
    <dbReference type="GeneID" id="20564691"/>
    <dbReference type="KEGG" id="ero:EROM_110920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110920"/>
    <dbReference type="HOGENOM" id="CLU_004790_3_1_1"/>
    <dbReference type="OrthoDB" id="8904098at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006857">
        <property type="term" value="P:oligopeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000109">
        <property type="entry name" value="POT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018456">
        <property type="entry name" value="PTR2_symporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11654">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00854">
        <property type="entry name" value="PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01022">
        <property type="entry name" value="PTR2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01023">
        <property type="entry name" value="PTR2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="443"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="472" mass="52850" checksum="DE26C641EA711BBB" modified="2012-10-03" version="1">MNRTTFFLIICNEFCERFCYYGLRSLLFSFAKEEYKFTVKESTIILHFFISMSYLFTLLGGFLSDMFLGRYNTIVVLSSLYLTGTSLLTYCSVVSGSPELLVTSLLMIAVGTGGIKPCVAAFGGDQFGPDEAQNLRKFFDFFYFTINIGSMVSMVVTPVMSSATCFGKQSCYPLAFGASSTLLGTSILLFATGTNLYVIKPARKRQFSRSFRSLVQNMKKLLWSMPGSDEEPDTLRTPSETACEVYEEDDKKRTLRILRILGPAVFFWALYDQQSSSWVVQGSKMNAHQSIFGYKVDVLPSQMQAFNSAFILLLIPVFSNLIYPLLGWIRVFSSPEEKMGVGIALASLSFFCSGYLEHRIGVAEFTGQKLSILWQIPQYIILTAGEIMLNVTGMEYMYAEAPETMKSVALSMWLLTVTAGNLLVMGIAFLDPLAFLPSFSHDMWSFVTYGIVGLVASKYMFQASRPIQKNTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQD2</accession>
    <name>I7AQD2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Spk1-like Ser/Thr protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_020470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264019.1">
        <property type="nucleotide sequence ID" value="XM_009265744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQD2"/>
    <dbReference type="GeneID" id="20520807"/>
    <dbReference type="KEGG" id="ero:EROM_020470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020470"/>
    <dbReference type="HOGENOM" id="CLU_531021_0_0_1"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF91">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="514" mass="58790" checksum="418E453202143DB3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQY2</accession>
    <name>I7AQY2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264229.1">
        <property type="nucleotide sequence ID" value="XM_009265954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQY2"/>
    <dbReference type="GeneID" id="20521023"/>
    <dbReference type="KEGG" id="ero:EROM_031100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031100"/>
    <dbReference type="HOGENOM" id="CLU_071105_0_0_1"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053299">
        <property type="entry name" value="ASTRA_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44156">
        <property type="entry name" value="SUPERNUMERARY LIMBS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="138"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="329" mass="37355" checksum="48CFF394F3646A13" modified="2012-10-03" version="1">MISSIGFLPSGISIEKLKKFVVDEDIHQSLNYLNIPVVDKEKVPFDDDIDESEEIMENDIVVFCTPNEDDVSFLQFYVQNRECTNMFLHHDTYVFSTICDSEYLTIGGEMYVALATFEKDIMVFDPFMRNPILPQVLLKGHEEAVLSIESRDGILFSGSLDSTIIEWDTEKALAKNRIQTMGPVNRIGILDSSVVYSVENSLYCLDEEIKFEGEVERIRIRENAMLVSNTTGTLRCYDLRKMTEVIIEKRIHEDSITGLDTLGDNVYTGSLDGTIKVVSLDTFGIVGSKDVDEKVFSLRAHEDGFYVYGGEKNELVLNYIDEPLQNDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQY5</accession>
    <name>I7AQY5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264234.1">
        <property type="nucleotide sequence ID" value="XM_009265959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQY5"/>
    <dbReference type="GeneID" id="20521028"/>
    <dbReference type="KEGG" id="ero:EROM_031150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031150"/>
    <dbReference type="HOGENOM" id="CLU_648946_0_0_1"/>
    <dbReference type="OrthoDB" id="2191092at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="426" mass="48655" checksum="C7CA0A2C6EBDCF1F" modified="2012-10-03" version="1">MRGESQDDYKPEQSKTLSSRAKGYKKKSLDIVRLERGRVTSQEILEKYRKPGIIEAMERPIESVVFRNKLSRVRGRIGFDDLALYNIGKCITAMSLPAKHSKYIFVGIKEGFQGESTFRFSSEASEVYLFDQSLNLRHVLSFKFGYCRKMETIQEKNVIRCVALFSDGFIRRFDFEEEIKNMTLIETEEMVNFEICWDYNLIFATDGKVLVWIQQNSVVSVFSGVKGMITSITIKRGALQEGRDEGCSLNCTPLDKSSLEFYALCISGKIFRFDYRFQEKKRISFPSGYTFIKYLSQVATLIIVDTLNNITKVLYDEGDSQRTSTMFNYSLSCCRSSDKRILIGGFDGTIRNAKVNKRKARVKTLFQVVRKGDEVILGHSEEEFKRLDSKGRPFNDNSERVVDLCMGGSDLFAAYECGIVVALGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARM8</accession>
    <name>I7ARM8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anaphase-promoting complex subunit 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_051010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the APC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264529.1">
        <property type="nucleotide sequence ID" value="XM_009266254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARM8"/>
    <dbReference type="GeneID" id="20521333"/>
    <dbReference type="KEGG" id="ero:EROM_051010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051010"/>
    <dbReference type="HOGENOM" id="CLU_039415_3_1_1"/>
    <dbReference type="OrthoDB" id="24948at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016901">
        <property type="entry name" value="APC10/Doc1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004939">
        <property type="entry name" value="APC_su10/DOC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12936:SF0">
        <property type="entry name" value="ANAPHASE-PROMOTING COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03256">
        <property type="entry name" value="ANAPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01337">
        <property type="entry name" value="APC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51284">
        <property type="entry name" value="DOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DOC" evidence="6">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="136" mass="15786" checksum="E4DF8BED3C5093E5" modified="2012-10-03" version="1">MEIRLSSFKREHSLKELLSKDMSEYWATDDVLPHSIQISFDKVKYVQSVQLFLSFTQDDSYTPEKIAIRSGLARECIKEISSVELVEPEGLLTLNVGRKCLYIQIVINSNHQEGKDSHIRHLKVLESPTKEIYYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARP8</accession>
    <name>I7ARP8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyrimidine 5-nucleotidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264549.1">
        <property type="nucleotide sequence ID" value="XM_009266274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARP8"/>
    <dbReference type="GeneID" id="20521353"/>
    <dbReference type="KEGG" id="ero:EROM_051210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051210"/>
    <dbReference type="HOGENOM" id="CLU_059493_1_0_1"/>
    <dbReference type="OrthoDB" id="2195201at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12725:SF117">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="324" mass="37034" checksum="4564D6377C934848" modified="2012-10-03" version="1">MRTIGNAAKAAFKVLSNGIKKAKHKAEKLVSGFGNKKKDASDSAEKSEGISFPVRSGIGDSEDCPQDYKDLLVEKPFKDPVFIEMGQDPDLISAEFRKIGKDVLFLYDIDDTLYHPSNNLQKMERKFLEEKYLSFKKDGTLETFKEELSLSLLYSSLFYNYVGISLEEYWEMLSEFDYLQYLSPDVNLRNFLLSMKNIRRCCFTNGPRDRAENILAKLGVLDCFEVVISIGKYDKTFCCKPLIKSYEFVTKVLGIESPGNVYFFDDSEINIIKAREFGWNGELITRDHNIIDVSSRILQEINEDARSLHFQKVSAVPSSKMPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARS2</accession>
    <name>I7ARS2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">20S proteasome sububit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_051470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264574.1">
        <property type="nucleotide sequence ID" value="XM_009266299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARS2"/>
    <dbReference type="GeneID" id="20521379"/>
    <dbReference type="KEGG" id="ero:EROM_051470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051470"/>
    <dbReference type="HOGENOM" id="CLU_035750_4_2_1"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000140">
        <property type="entry name" value="Proteasome endopeptidase complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="3">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="232" mass="26281" checksum="77947CD9A1F34569" modified="2012-10-03" version="1">MEDFKQSVNTYSSEGRIHQIEYAMKAMNLGTTTIGVRTKEFVILCSEKKILSTLQNPKSVVKHYKIYDHIVLGFSGISGDTKTIVKRSRDFCISHTHMYGENTSVERLLKYLSSLSLRFGEEDEAKMIFRRPFGVSLIIAGFDTEPRLYSLDPSGSYVSYKARAIGSGHEVVEGVLENEYEEYRGLDQSLRKALHTLSKVMKDRINKDNVEVAVVTKEGSKFLTPEDISAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS22</accession>
    <name>I7AS22_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264669.1">
        <property type="nucleotide sequence ID" value="XM_009266394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS22"/>
    <dbReference type="GeneID" id="20521475"/>
    <dbReference type="KEGG" id="ero:EROM_060790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060790"/>
    <dbReference type="HOGENOM" id="CLU_1907267_0_0_1"/>
    <dbReference type="OrthoDB" id="2189154at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="152" mass="17726" checksum="954543F94733F565" modified="2012-10-03" version="1">MISCVYPFMDRLYKDVSEEFLSGLKRYINDEISYSELERLSTRESLAFNSHKWNEVIEKKSLEALGIKRRMYDEILQIEEHIKTMEKLEKGEDFDVDLEGLLSHSGIVGRNRSHPPGYENTSLYLPPFPSLPMVSFLNDSETESSEEGRRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS48</accession>
    <name>I7AS48_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264714.1">
        <property type="nucleotide sequence ID" value="XM_009266439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS48"/>
    <dbReference type="GeneID" id="20521522"/>
    <dbReference type="KEGG" id="ero:EROM_061260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061260"/>
    <dbReference type="HOGENOM" id="CLU_1077795_0_0_1"/>
    <dbReference type="OrthoDB" id="2190375at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14938">
        <property type="entry name" value="SNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="258" mass="29741" checksum="3CCD1B7768F2FCCF" modified="2012-10-03" version="1">MDAESKNRKLKEAEKLRTKGTFPLSLFSSPDYDASAEIFMEVGNMEEDLSERVRYYEEAAKTYEMEEGEYGRYQASQVYEKIAQICEDEHPKKSIAAYKRSGMYSKQCGRESLAAISFQRASEILKKEGDLNGCLECLQKVAECYCGSNWKHHKRKVMRDIADVYIELGKYGLAAKVFLSFKENIYNFCAFLCYAIEGSPLDLDVDGDEREICDALERDFNEAHKTIEEYITTHAMITEVRKLLEIVKEKLKPENDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASZ4</accession>
    <name>I7ASZ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional activator Spt7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_081560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265069.1">
        <property type="nucleotide sequence ID" value="XM_009266794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASZ4"/>
    <dbReference type="GeneID" id="20521891"/>
    <dbReference type="KEGG" id="ero:EROM_081560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081560"/>
    <dbReference type="HOGENOM" id="CLU_1354603_0_0_1"/>
    <dbReference type="OrthoDB" id="21449at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031498">
        <property type="entry name" value="Bromo_TP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037782">
        <property type="entry name" value="Spt7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47343:SF1">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR SPT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17027">
        <property type="entry name" value="Bromo_TP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="3">
        <location>
            <begin position="43"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="203" mass="23462" checksum="CAAC91C967D38A7D" modified="2012-10-03" version="1">MVGKRGCNLFILGLISSSEEKRRFLDRIFFLPERLTRILTAIKAFEQSKIFLSRVLKKDAPNYYEIIKRPMDLSIVQKKIGKYKSFEEFKGDLDLIWDNCLKFNHSKYHRDCAFKMKEVVSTFEIEVIPVSMDTGFATSSHVWEGEEGQGPIIKYMVKKIISEVLLSSGYGFASETALGVFCDVFQYRILKIIKKIKAEEMDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATM0</accession>
    <name>I7ATM0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_092030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265314.1">
        <property type="nucleotide sequence ID" value="XM_009267039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATM0"/>
    <dbReference type="GeneID" id="20564427"/>
    <dbReference type="KEGG" id="ero:EROM_092030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_092030"/>
    <dbReference type="HOGENOM" id="CLU_1124524_0_0_1"/>
    <dbReference type="OrthoDB" id="2192751at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="245" mass="28756" checksum="63CB8CCCE8B2F382" modified="2012-10-03" version="1">MINEIKSFLPSPRSLESIEPIKKRAKRKERPSKHVLSKSIYEETDLSKLTPRQRVLHQKYQESKNNVTSFFLLSNNILINSTNHRPKEDKGKMVCNKFTHKGVDEHCYGEHRDDLQKESRFLVVTSDMYNSSYNDTKEESTSSEDGCKVIEAMDVESPFDNKWPEDVRYKDCLICKECFRDAARAYYALEEIGNMHKCGYQNSIMLHKYKIRQNFKKQVCFSVIRKEEIVFIKSLEQLKCCNSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">ADP,ATP carrier protein</fullName>
        </recommendedName>
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        <name type="common">Microsporidian parasite</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
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    <comment type="similarity">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATP3"/>
    <dbReference type="GeneID" id="20564460"/>
    <dbReference type="KEGG" id="ero:EROM_100300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100300"/>
    <dbReference type="HOGENOM" id="CLU_023964_1_0_1"/>
    <dbReference type="OrthoDB" id="2190844at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004667">
        <property type="entry name" value="ADP_ATP_car_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187:SF1">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03219">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="536" mass="61390" checksum="DEB6E87D59152531" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATQ0</accession>
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        <recommendedName>
            <fullName evidence="9">ADP,ATP carrier protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="10" type="ordered locus">EROM_100410</name>
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    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ADP/ATP translocase tlc family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265354.1">
        <property type="nucleotide sequence ID" value="XM_009267079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATQ0"/>
    <dbReference type="GeneID" id="20564471"/>
    <dbReference type="KEGG" id="ero:EROM_100410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100410"/>
    <dbReference type="HOGENOM" id="CLU_023964_1_0_1"/>
    <dbReference type="OrthoDB" id="2190844at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="ADP_ATP_car_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187:SF1">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03219">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="503"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="552" mass="62051" checksum="EBFBC828A63CFB5F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATR9</accession>
    <name>I7ATR9_ENCRO</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009267099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATR9"/>
    <dbReference type="GeneID" id="20564491"/>
    <dbReference type="KEGG" id="ero:EROM_100610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100610"/>
    <dbReference type="HOGENOM" id="CLU_128337_0_0_1"/>
    <dbReference type="OrthoDB" id="2189928at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="165" mass="19315" checksum="58207105AE15E429" modified="2012-10-03" version="1">MLIVNRARLRRNSIIELCVFPDMNVHDTMVELSRQTDKPVFFDTVGNLRFVADALYILSYRKLIKALLSLRSKQGFVLFIDSISFLADKSMESLQRVYNVLWMLIYENDATVVVSNHYRIASNTSGTEFVPRLGKRWKMMVSYRIMYGYDGDKIVTRVYSDELFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATV3</accession>
    <name>I7ATV3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Origin recognition complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_101020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the origin recognition complex (ORC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ORC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265414.1">
        <property type="nucleotide sequence ID" value="XM_009267139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATV3"/>
    <dbReference type="GeneID" id="20564532"/>
    <dbReference type="KEGG" id="ero:EROM_101020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101020"/>
    <dbReference type="HOGENOM" id="CLU_1138763_0_0_1"/>
    <dbReference type="OrthoDB" id="346673at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007220">
        <property type="entry name" value="ORC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056772">
        <property type="entry name" value="RecA-like_ORC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14052:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04084">
        <property type="entry name" value="RecA-like_ORC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Origin recognition complex subunit 2 RecA-like" evidence="2">
        <location>
            <begin position="13"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="254" mass="29204" checksum="DA18BFE572612DF3" modified="2012-10-03" version="1">MSQNLTDFHLNCKKEYNVLTRSFNILFYGYGSKKPLLKKMFPTAIYLNCRTMGRHEILAEIVDTVRRRSRLETQRVSKTSTIKDIDEAIGTRKEKYKLIMANFDFSMLEFSGLKNFAILGTIEGVDIRFSLEDIERFNFIFRDLTTFDPYEEEIIGIHLGATKVEASSRVVSSVPRNSRVVLKEILLCNEDTVSLSELFERTKRKLFLTSKASVLSMISEFIDHGLLKIKNGTEVVVCMSPTEKKEIAKELDCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATX5</accession>
    <name>I7ATX5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ercc1-like DNA excision repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_101220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERCC1/RAD10/SWI10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265434.1">
        <property type="nucleotide sequence ID" value="XM_009267159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATX5"/>
    <dbReference type="GeneID" id="20564552"/>
    <dbReference type="KEGG" id="ero:EROM_101220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101220"/>
    <dbReference type="HOGENOM" id="CLU_041616_3_3_1"/>
    <dbReference type="OrthoDB" id="10262814at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22325">
        <property type="entry name" value="ERCC1_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047260">
        <property type="entry name" value="ERCC1-like_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004579">
        <property type="entry name" value="ERCC1/RAD10/SWI10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00597">
        <property type="entry name" value="rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749:SF0">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12749">
        <property type="entry name" value="EXCISION REPAIR CROSS-COMPLEMENTING 1 ERCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03834">
        <property type="entry name" value="Rad10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC1-like central" evidence="7">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="187" mass="21608" checksum="986527520FD49B8A" modified="2012-10-03" version="1">MIKVSPLQKGNSVLDYLSNATWHYDNSITPDYEVNGSVALLFLSLRFHSCKPEYIYKRINKLKPYKVRVLLVHVDVPNYSKMIRELFRTVSLTMVLGFSVEECSRYIQGFDAAGRRSIDIIRRGSRDEEGFLCSFPKVNKSDVQQILKDCGTLQKFFGKSSEEMERIQGLGKSKAEEIIKYLNMQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATZ1</accession>
    <name>I7ATZ1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TALE/PBX transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_101370</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265449.1">
        <property type="nucleotide sequence ID" value="XM_009267174.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATZ1"/>
    <dbReference type="GeneID" id="20564567"/>
    <dbReference type="KEGG" id="ero:EROM_101370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101370"/>
    <dbReference type="HOGENOM" id="CLU_106819_0_0_1"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="117"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="119"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="189" mass="22174" checksum="63E30EF7CE922A22" modified="2012-10-03" version="1">MNIIMDILNRVVYAEEDYLANRVGRERLLEDIKHLRKEYKTSISSDSGREGIKFKMALLFVLRRVKHKILFEDRLVEVIDSEITFIKECVKSIISEPIRTGLVEATKYIQHETERKCSKPKTRANFPMDTSQLLRSWLKENMDNPYPSDAEKAYLCQKTGLGPAQINNWFINARRRILPFMKGKCTNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU07</accession>
    <name>I7AU07_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-mRNA cleavage complex 2 protein Pcf11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265464.1">
        <property type="nucleotide sequence ID" value="XM_009267189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU07"/>
    <dbReference type="GeneID" id="20564582"/>
    <dbReference type="KEGG" id="ero:EROM_101520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101520"/>
    <dbReference type="HOGENOM" id="CLU_915358_0_0_1"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="35300" checksum="0E521AB15DA450DA" modified="2012-10-03" version="1">MDEFISLLKILKKNDKIAQNVLLMISESFRYNEEVMSDALIKHHRSLLSKEPVLDFLFLLCNKDARYLNLFRRYKEEFDTDHKISRLLSGEDLEERPKKPKVDSKGNSKPADIKGCSDAKHFPVNPVSDSNPKDKEPSNKRIGQNVFEEAKSKNSWDRKENELGILNNEYLKIDVDPSVLYPPNQCKLCGLRYEDTPEGDEQMRIHIDEHRRKARVLGEKDCISREFFPTLEAWTKNIEKIKLRLEVEKVEKIAHSGGPALCDVCRNKIEVEWDDQEDRWILKDAISLEKGGRTTFCHRKCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU98</accession>
    <name>I7AU98_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_110750</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265554.1">
        <property type="nucleotide sequence ID" value="XM_009267279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU98"/>
    <dbReference type="GeneID" id="20564674"/>
    <dbReference type="KEGG" id="ero:EROM_110750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110750"/>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_1"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="2"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="312"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN84057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="535" mass="59390" checksum="657A552D1D52939E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUD5</accession>
    <name>I7AUD5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111100</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265589.1">
        <property type="nucleotide sequence ID" value="XM_009267314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUD5"/>
    <dbReference type="GeneID" id="20564709"/>
    <dbReference type="KEGG" id="ero:EROM_111100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111100"/>
    <dbReference type="HOGENOM" id="CLU_1094271_0_0_1"/>
    <dbReference type="OrthoDB" id="2192267at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019407">
        <property type="entry name" value="CTU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20882:SF14">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="247" mass="27845" checksum="76F4A7F06FA8826C" modified="2012-10-03" version="1">MEHRCVICTYTPNIRINNLDYCNTCFERQLLHKVFKHFKKLPFGSRILLYFDGTFSSLAMAHAISRLKNRNIHKFVVASRSWPEHRDFLKDIGFSCHVDLQPESDVSNAGLCRSKIDPIPKEVIEVGRSLGFDTVVFQADAEMESVIALRSVCDGCGVLETIESYVSGISGVRVENAFGRIKPKEVRYYCYLNKIAGSFTKLGMSSKELVLSRFISKMESKNSLVVFNILNTVKKIVAGKDGIDTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUK2</accession>
    <name>I7AUK2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">20S proteasome subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265634.1">
        <property type="nucleotide sequence ID" value="XM_009267359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUK2"/>
    <dbReference type="GeneID" id="20564755"/>
    <dbReference type="KEGG" id="ero:EROM_111560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111560"/>
    <dbReference type="HOGENOM" id="CLU_035750_8_1_1"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="230" mass="26182" checksum="E93F0E8BE3618127" modified="2012-10-03" version="1">MGSQARYNMFKIFNPEGCVKQLDFIQQTTELGNTVVGLRNKKVGILIAHNEKRSRLADAQKKVFSIDKRSLFTFSGITNDGEQIVDYLIDKSIREEIIKGRPTHFLDVFEDLLRDTFERTIVDGRRLYGVSGLYMTEYEGIRLVEFNPKGYAREVNGMSIGCRSQSSRTVLEAYCEKYENMNTEELVRVGIMALKNAHPEDDALTSENVEIWVLEVGKAVHNIDAGVFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGR5</accession>
    <name>I6ZGR5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Small nuclear Sm-like ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010330</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263875.1">
        <property type="nucleotide sequence ID" value="XM_009265600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGR5"/>
    <dbReference type="GeneID" id="20520660"/>
    <dbReference type="KEGG" id="ero:EROM_010330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010330"/>
    <dbReference type="HOGENOM" id="CLU_123956_2_0_1"/>
    <dbReference type="OrthoDB" id="2190807at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="5">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="99" mass="11547" checksum="27B8D2178CB05DF5" modified="2012-10-03" version="1">MKLIRFLKKCKGEKVKVETKEGRKIEGRITSVDKTMNVEMEDAVVDGVPASTYTVRGSSIRYIMFRDDIDFKPLLVDDRPRNRTKDQEATRVKRRKTKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHB7</accession>
    <name>I6ZHB7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dim1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_021180</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DIM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264090.1">
        <property type="nucleotide sequence ID" value="XM_009265815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHB7"/>
    <dbReference type="GeneID" id="20520880"/>
    <dbReference type="KEGG" id="ero:EROM_021180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021180"/>
    <dbReference type="HOGENOM" id="CLU_1825858_0_0_1"/>
    <dbReference type="OrthoDB" id="2188851at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004123">
        <property type="entry name" value="Dim1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052:SF5">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12052">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEN 4A, 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02966">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01410">
        <property type="entry name" value="DIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="140" mass="15885" checksum="9A9F7063483ABE27" modified="2012-10-03" version="1">MVNVLDSLDEVNRVVGDTLCRLVVIRFGDRDDPLCIHVDAVLERICPTLSKYVDIYTCERASVRELVDVMKLDSPLNIMCFLNRRHIKIDCSSGDNDKINFLVDDPEMLIELFTLAYKAGVRGKGIIRSPFKIRELQGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHU4</accession>
    <name>I6ZHU4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S14p/S29e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_040070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264275.1">
        <property type="nucleotide sequence ID" value="XM_009266000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHU4"/>
    <dbReference type="GeneID" id="20521071"/>
    <dbReference type="KEGG" id="ero:EROM_040070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040070"/>
    <dbReference type="HOGENOM" id="CLU_177289_2_0_1"/>
    <dbReference type="OrthoDB" id="10252683at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="66" mass="7746" checksum="FD47051AE368D070" modified="2012-10-03" version="1">MSFEVLFDVPVKKNERSGPHSHRMPFGKGSRSCVSCYTFRGIIRKLMMCRRCFREYASDIGFTIYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI30</accession>
    <name>I6ZI30_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">KTR mannosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_041110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264375.1">
        <property type="nucleotide sequence ID" value="XM_009266100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI30"/>
    <dbReference type="GeneID" id="20521175"/>
    <dbReference type="KEGG" id="ero:EROM_041110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041110"/>
    <dbReference type="HOGENOM" id="CLU_024327_4_0_1"/>
    <dbReference type="OrthoDB" id="439943at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000032">
        <property type="term" value="P:cell wall mannoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000051">
        <property type="entry name" value="Alpha-1,2-mannosyltransferase (Ktr4)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002685">
        <property type="entry name" value="Glyco_trans_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31121:SF6">
        <property type="entry name" value="ALPHA-1,2 MANNOSYLTRANSFERASE KTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01793">
        <property type="entry name" value="Glyco_transf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018153">
        <property type="entry name" value="Glyco_trans_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707005" evidence="4">
        <location>
            <begin position="18"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR018153-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="322" mass="37789" checksum="FF531EA1B44546D1" modified="2012-10-03" version="1">MLLLLVIIGMGICKENAVILILCRNSDKNGITETIKNFEEMFNKNFGYPYVFLNDEEFTEDFKKSIKGITSSSVEFGKLDPEEWEVPPWIDMEKAKQGMKNLEERKIIYGGSLSYRKMCRFFSGFFYKNKLVQKYDYYWRIEPDVKFLCQIEYDPFTYLRENNKQYGFVISLVEFMETIPSLFGEAIKFATKNLNLIKKANGNRFILNKDGSYNGCHFWSNFEIANFGFFRSETYQKYFDWLDRAGGFFYERWGDAPVHSIAASLFLDKDEIHFFSDIGYEHPPFRHCPREASMKSKCKCSITDSMDISSNTCLGMYIKSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI63</accession>
    <name>I6ZI63_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cyclin B-like guanine nucleotide binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264410.1">
        <property type="nucleotide sequence ID" value="XM_009266135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI63"/>
    <dbReference type="GeneID" id="20521212"/>
    <dbReference type="KEGG" id="ero:EROM_041490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041490"/>
    <dbReference type="HOGENOM" id="CLU_401715_0_0_1"/>
    <dbReference type="OrthoDB" id="2190070at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cDENN" evidence="1">
        <location>
            <begin position="159"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="685" mass="80164" checksum="F7D39A82F69965BC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIB9</accession>
    <name>I6ZIB9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MAGE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264465.1">
        <property type="nucleotide sequence ID" value="XM_009266190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIB9"/>
    <dbReference type="GeneID" id="20521267"/>
    <dbReference type="KEGG" id="ero:EROM_050350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050350"/>
    <dbReference type="HOGENOM" id="CLU_127769_0_0_1"/>
    <dbReference type="OrthoDB" id="2191533at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="192" mass="22235" checksum="D121E2C3AF0A50DF" modified="2012-10-03" version="1">MAGKITALPFLMNDNETLAGEFVRILMDNHRKSTPTRKQSVRAQLKVGLKEMGALVELSKGYLEKLGLELVGIGKEGVIDPMTAEKYFIRRIKPSPATEKFLPEETQRLILAFTFLILERKVIEVPRLWFFMQKTGVFESEDDFAEFLNQTKRQGYLFVTKVEESLIITPGWRYHCDFHNFDPKGYFRNNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZII9</accession>
    <name>I6ZII9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spore wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264535.1">
        <property type="nucleotide sequence ID" value="XM_009266260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZII9"/>
    <dbReference type="GeneID" id="20521339"/>
    <dbReference type="KEGG" id="ero:EROM_051070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051070"/>
    <dbReference type="HOGENOM" id="CLU_1107121_0_0_1"/>
    <dbReference type="OrthoDB" id="2192251at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012135759" description="Spore wall protein" evidence="1">
        <location>
            <begin position="16"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29845" checksum="A13B8B0D29BC2AE3" modified="2012-10-03" version="1">MQVLALLGLFAFATCKDTKEKKRLVHVSCHYEDHLNVNYVDEFKRLNSSNEDNKMISKCTYLNKIYTMCKSAYKEAGERITGERSEYILLVLNFLYDYCSARTYELGAVTALVLHNTSYLRVFEGNEYSEFKARGIFHVYGEENYKFLGKVSFFYRDYYQNPERASHLNIYVLVDTACFWLHSSCHKKTEIGLNDALEVCNHVQWKILREKWNYSSSRVRKAEEEYAIQHELYEKLRTIIYINYYRDLDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ11</accession>
    <name>I6ZJ11_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-mRNA-splicing factor CWC24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_061010</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the CWC24 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN83193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266415.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ11"/>
    <dbReference type="GeneID" id="20521497"/>
    <dbReference type="KEGG" id="ero:EROM_061010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061010"/>
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    <dbReference type="OrthoDB" id="25761at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034247">
        <property type="term" value="P:snoRNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039971">
        <property type="entry name" value="CWC24-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930:SF0">
        <property type="entry name" value="RING FINGER PROTEIN 113B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12930">
        <property type="entry name" value="ZINC FINGER PROTEIN 183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="4 5 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="8">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="101" mass="11299" checksum="8AEB078F65E5485E" modified="2012-10-03" version="1">MLEDNEVVDTHKIICKSFRETGYCGYGDSCKYSHDRSMGYSESPVVSDTGLLCGICRKLYKERVVTECGHSFCSLCAIRKYQNGDECGVCGKPVYGKFWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ28</accession>
    <name>I6ZJ28_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_061270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264715.1">
        <property type="nucleotide sequence ID" value="XM_009266440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ28"/>
    <dbReference type="GeneID" id="20521523"/>
    <dbReference type="KEGG" id="ero:EROM_061270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061270"/>
    <dbReference type="HOGENOM" id="CLU_022332_1_3_1"/>
    <dbReference type="OrthoDB" id="18894at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="6">
        <location>
            <begin position="6"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="307" mass="34120" checksum="2E9E8589D2E35980" modified="2012-10-03" version="1">MNRIRVLGLVLMYYILSLSLGYLTSFLLSRKTYNFSFPLFKASLQNLIHFLGASTALRLQREKAPKPNYFGPSFPCALAGATDIGLSSISLRFTTLAFYTMVKSSSPVFILLFGFALGIENPSITFFLTIFTIGVGVFLTSIKNTSFDIIGFGTISLASLMAGFRWAFVQYLIRNQEVRKEGVFVTMRDLCLPISFLLLLISLHVEGLMEIVTSRFFNTFDAALRNFGYILLCGTLSFMLLCAEFSLVSETGVVFLSVAGIVKELIIIFYAIITKDIILSNLNYAGLGISIMGILVYNLSRKPFSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ47</accession>
    <name>I6ZJ47_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleoside diphosphate kinase</fullName>
            <ecNumber evidence="3">2.7.4.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_061520</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264740.1">
        <property type="nucleotide sequence ID" value="XM_009266465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ47"/>
    <dbReference type="GeneID" id="20521549"/>
    <dbReference type="KEGG" id="ero:EROM_061520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061520"/>
    <dbReference type="HOGENOM" id="CLU_060216_6_3_1"/>
    <dbReference type="OrthoDB" id="2162449at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000002">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="7">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="147" mass="16634" checksum="5AA8FF26F5B2BA9B" modified="2012-10-03" version="1">MERTFIMIKPDGIKRRLISRIIQRFEEKGLYLAASKCVIPKRDVLEQHYSHLSSKPFFNEIVESMMSGMVLAMVWVGKNAVSTGRKLIGETDPQAASIGTIRGDYGITVGKNIIHGSDTVENAEREIKLWIGDDVQPVSFFDEAWIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ66</accession>
    <name>I6ZJ66_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Translocase Tim22/17-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264765.1">
        <property type="nucleotide sequence ID" value="XM_009266490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ66"/>
    <dbReference type="GeneID" id="20521576"/>
    <dbReference type="KEGG" id="ero:EROM_070170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070170"/>
    <dbReference type="HOGENOM" id="CLU_164171_0_0_1"/>
    <dbReference type="OrthoDB" id="75343at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="139" mass="14971" checksum="CF0B8824AC862CF4" modified="2012-10-03" version="1">MKISRNNFQKNQVPNMNMKSIREKIEKVKPHFLKVASDTLQGYAFGCMVGVFSSSKKPTLSSIHESGKSFAKISLVYSTTESSLQLYGSENAPLNSFISGVVAGGLGVNDKSKRTIFAGAASFGLYTGMSSAFSVRGQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ83</accession>
    <name>I6ZJ83_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264790.1">
        <property type="nucleotide sequence ID" value="XM_009266515.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ83"/>
    <dbReference type="GeneID" id="20521601"/>
    <dbReference type="KEGG" id="ero:EROM_070420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070420"/>
    <dbReference type="HOGENOM" id="CLU_1959557_0_0_1"/>
    <dbReference type="OrthoDB" id="2189011at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031495">
        <property type="entry name" value="DUF5100"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17029">
        <property type="entry name" value="DUF5100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="128" mass="14773" checksum="8B6C3550F89A516C" modified="2012-10-03" version="1">MSIKSKYNKVMKALTIRQNDLWLLPKLITPLENSFQSEAINDEEICIVPCEANFQLPNIFLKTGLNPEYQKLIDTSGCSEDWNDIIEEAIEALEEIDLSSINHNIMTKKCLVDQDTLNRLVFERYGHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ93</accession>
    <name>I6ZJ93_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264800.1">
        <property type="nucleotide sequence ID" value="XM_009266525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ93"/>
    <dbReference type="GeneID" id="20521611"/>
    <dbReference type="KEGG" id="ero:EROM_070520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070520"/>
    <dbReference type="HOGENOM" id="CLU_535307_0_0_1"/>
    <dbReference type="OrthoDB" id="2190909at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="509" mass="58650" checksum="91167390CC3DEBDB" modified="2012-10-03" version="1">MKRSIKVCHSKTHGFPLAENISHHELRIDHNVLSTLKLSLLPGSYIRVELFPTPKSIFINDSFNMIDVYSVYKDGVLFFNQKPIPWESDGQISWEKYKEKLIIYVSETLAHGMEYIEGLNDRYVFYTKYNLLNPINVNQCRKELQKLSREFELMNRIEGLQLSTPKEVGKPFVEEAYDVLLSYRGNPDLGIRFILEAIDVLPFFTITKMAKSIVKARRYQAVLLYYRVYLKSLEKERYRTAFLFGISAARLLKQGTSIGLEETIKLEIPRCVKERNWRMVLRDVMNKIEVLDSSKYGTNPYRLFDVDGSEFTVGTVELAQSDIVKRSGNTVLFDGENTKVYFTDRFRIKITFSRISHTSLHGILASTISASRIFIPIYKTLYESPATVNVFFNFSQLLIENEADEAEIRLEELIFSGRIIVPIDLAITWVRKKSTIWVKSVSPVDEDGRVTFLLALSNLKGFESSVLGPDVVERKGCVVAVSSHLSQRSKTCLHHEISENIFETFELVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJB8</accession>
    <name>I6ZJB8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264830.1">
        <property type="nucleotide sequence ID" value="XM_009266555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJB8"/>
    <dbReference type="GeneID" id="20521641"/>
    <dbReference type="KEGG" id="ero:EROM_070820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070820"/>
    <dbReference type="HOGENOM" id="CLU_1396301_0_0_1"/>
    <dbReference type="OrthoDB" id="2019504at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="195" mass="22850" checksum="371CE03331CDFFC8" modified="2012-10-03" version="1">MDVQKTIDAIRSNDIETNKAAIETIYANYGLLKEEDIVRLTPSIAYYLWKLPKFVAQKQFVLDLLSHTDQRLRHSMFMYLIDKWSEIQLVRMDKFYYIMKRILEYYTVDVETVSYLFNIPTVMELKAFIIRCVVDRLGETSEDMEHFLAEFISKCPPALLLSLEPLKIRKEIALKYAGSKDVGKKNREALCSMAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJE1</accession>
    <name>I6ZJE1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264855.1">
        <property type="nucleotide sequence ID" value="XM_009266580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJE1"/>
    <dbReference type="GeneID" id="20521667"/>
    <dbReference type="KEGG" id="ero:EROM_071070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071070"/>
    <dbReference type="HOGENOM" id="CLU_600058_0_0_1"/>
    <dbReference type="OrthoDB" id="2196200at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="422" mass="49992" checksum="664B6AD1DC511601" modified="2012-10-03" version="1">MKCIEEYTFDRDKCPGKMLEDILREDYSFDFEGEQKSLFTLRKKFLWYEGCKVIEVGEVPGHIHGNRLFMVEDRLRVYELPSVASRFRNASVAKKEKTVCIERDGRKCFLPDGAEDGFLDGDFSKDRVWIWSLAEEEGREYLSRKVYLLRKEELVGPEKKLYKRNGEVILQIQDIFVRKCVEISNPMRVWSKAMGDGMVVIVVTSGIVYMEYGGYVSSKPCCKGIYGMDGCRLLGLSSWTDISDLYLTLRLFTDIQVDLRALERIEEYLFKLFVFTSKGEWMVKWLLKSGMDKEKEMILCRLYRKVDDKGKRKLNPWMERIISLDALKLVIIYFPEEMERYIRMCIEEKREYEIEEIIEHYRGSEMIERICQILLRNNCLYLFSLCMPEYVGKFGDVALVEKYNMESQKNKWKVQKMEENLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJM6</accession>
    <name>I6ZJM6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Coatomer subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_080260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8 9">Cytoplasmic vesicle</location>
            <location evidence="8 9">COPI-coated vesicle membrane</location>
            <topology evidence="8 9">Peripheral membrane protein</topology>
            <orientation evidence="8 9">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8 9">Golgi apparatus membrane</location>
            <topology evidence="8 9">Peripheral membrane protein</topology>
            <orientation evidence="8 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the adaptor complexes medium subunit family. Delta-COP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264945.1">
        <property type="nucleotide sequence ID" value="XM_009266670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJM6"/>
    <dbReference type="GeneID" id="20521762"/>
    <dbReference type="KEGG" id="ero:EROM_080260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080260"/>
    <dbReference type="HOGENOM" id="CLU_667471_0_0_1"/>
    <dbReference type="OrthoDB" id="10266042at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051645">
        <property type="term" value="P:Golgi localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09254">
        <property type="entry name" value="AP_delta-COPI_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027059">
        <property type="entry name" value="Coatomer_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10121:SF0">
        <property type="entry name" value="COATOMER SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="6 8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="196"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="420" mass="48037" checksum="BCE20C65240CFC58" modified="2012-10-03" version="1">MIIGLFVANSLTGQTVSRMTRAMAVSKIEHFILDFISSGVSASGVLPKGFYKYVYSMADSTYYVVIASMGYNNVKAQGILDLLQKNTDGDMVDALVTMDNILYGQTTFDPNIALIKSMDSQEEKIHELMMKNKNKELIQKQKELQRRPIGDIDRELERVRNLEIEMRNENIQQLKSIVRTGGNPAKEKRRELETSMSSVFILFKERLKMVIDKENNIKSGEVQGDMSITIREEEYKDIEIKLGNVDKDMKFSPNLDKSVSNEGILRCEKGFPIEKNVALVKWKGSDIREPPITFTFWPSETSLNVYQIMFEYTAECNMKDLNVFFSKANISNIKINGTAKERDSHIEWSIGDVEKGTSDTLEFSCTCTDPSEIFPLEIYFTSDFVLTRLFVEKVTRCGEEVDELEIKKVFEVDKFTVVDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJQ9</accession>
    <name>I6ZJQ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Vesicle coat complex subunit zeta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264980.1">
        <property type="nucleotide sequence ID" value="XM_009266705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJQ9"/>
    <dbReference type="GeneID" id="20521800"/>
    <dbReference type="KEGG" id="ero:EROM_080640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080640"/>
    <dbReference type="HOGENOM" id="CLU_129942_0_0_1"/>
    <dbReference type="OrthoDB" id="10249988at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="165" mass="18819" checksum="02B12236A5A3844E" modified="2012-10-03" version="1">MSLFDVEGLVVVDVQGEILYKRIFSKEEDIAAKIVEKSTKDRDIISVSHERIVMCKKLDEILLVIYSPLEVNEAFIGQVFDEFTTAFVSVIKTPTRERVWKKYDQIVLLVSTFLYEGIVMVGKSDEMLNRLPKRNFEGVDGIKVPKGFASFLHKATKSLSINNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJU9</accession>
    <name>I6ZJU9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L23</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265030.1">
        <property type="nucleotide sequence ID" value="XM_009266755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJU9"/>
    <dbReference type="GeneID" id="20521852"/>
    <dbReference type="KEGG" id="ero:EROM_081170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081170"/>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_1"/>
    <dbReference type="OrthoDB" id="407959at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="146" mass="16290" checksum="AE676AD278616BBF" modified="2012-10-03" version="1">MAAEKKTEVLEKKISIKPRYKMSRGVQVESLLKCADNSGAKILRCIGVKRYRGRLNRLPAAAPGDICVVSVKKGKPELRKKVHYAILIRQKKIWRRTDGSHIMFEDNAAVLINNKGELRGAQIAGPVPREVADMWPKISSQASSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK56</accession>
    <name>I6ZK56_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265125.1">
        <property type="nucleotide sequence ID" value="XM_009266850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK56"/>
    <dbReference type="GeneID" id="20521949"/>
    <dbReference type="KEGG" id="ero:EROM_090100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090100"/>
    <dbReference type="HOGENOM" id="CLU_117729_0_0_1"/>
    <dbReference type="OrthoDB" id="2194483at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="201" mass="23922" checksum="EA5099C289207767" modified="2012-10-03" version="1">MSIYLEKVRKIMDEFEGEDKEALIKHYIRVSKNVLLDDKEVKRSKLNLLGDLYAIDGGDEVNAIMNDVLEHKILQIRALILDLVEDDYTSDSKVIGRPEKWIKKIIKDAEETFNLDGEFGKRMFSIYNEKLLKEFCRIFISENRRFGTGGNQLLLNLYYYERFVQSKIKFDFQNFFDRMTSFFRDHCYKPKEELEEILDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKK8</accession>
    <name>I6ZKK8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">P-loop ATPase/acetyltransferase fusion</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_100150</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265330.1">
        <property type="nucleotide sequence ID" value="XM_009267055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKK8"/>
    <dbReference type="GeneID" id="20564445"/>
    <dbReference type="KEGG" id="ero:EROM_100150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100150"/>
    <dbReference type="HOGENOM" id="CLU_004652_0_0_1"/>
    <dbReference type="OrthoDB" id="10067491at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990883">
        <property type="term" value="F:18S rRNA cytidine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904812">
        <property type="term" value="P:rRNA acetylation involved in maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007807">
        <property type="entry name" value="TcmA/NAT10_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032672">
        <property type="entry name" value="TmcA/NAT10/Kre33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013562">
        <property type="entry name" value="TmcA/NAT10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027992">
        <property type="entry name" value="tRNA_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925">
        <property type="entry name" value="N-ACETYLTRANSFERASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10925:SF5">
        <property type="entry name" value="RNA CYTIDINE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13718">
        <property type="entry name" value="GNAT_acetyltr_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05127">
        <property type="entry name" value="NAT10_TcmA_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08351">
        <property type="entry name" value="TmcA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13725">
        <property type="entry name" value="tRNA_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="9">
        <location>
            <begin position="514"/>
            <end position="679"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="825" mass="93406" checksum="CBA0CB5FD1A0F306" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKM1</accession>
    <name>I6ZKM1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265350.1">
        <property type="nucleotide sequence ID" value="XM_009267075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKM1"/>
    <dbReference type="GeneID" id="20564467"/>
    <dbReference type="KEGG" id="ero:EROM_100370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100370"/>
    <dbReference type="HOGENOM" id="CLU_289505_0_0_1"/>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1059" mass="126166" checksum="9F6A60B5B0064A4C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKQ6</accession>
    <name>I6ZKQ6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_100820</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265395.1">
        <property type="nucleotide sequence ID" value="XM_009267120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKQ6"/>
    <dbReference type="GeneID" id="20564512"/>
    <dbReference type="KEGG" id="ero:EROM_100820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100820"/>
    <dbReference type="HOGENOM" id="CLU_030988_13_3_1"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000060">
        <property type="entry name" value="Ubiquitin conjugating enzyme (UbcB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="6">
        <location>
            <begin position="6"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="158" mass="17917" checksum="D7A830C3CE0BCB63" modified="2012-10-03" version="1">MATTNGKNSRLAIEMESMSRDPPPGCSASPISPNELDKWIAVITGPPGSVYEDGIFELLIVFPPFYPFSPPKVTFKTRIFHCNIFEKNICLDILKDQWSPALTIDKILLSIISLLQDPNSDDPLNKEAADLYRNNRAEYNRKAKEWVRLHASKHKSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL80</accession>
    <name>I6ZL80_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_111010</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265580.1">
        <property type="nucleotide sequence ID" value="XM_009267305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL80"/>
    <dbReference type="GeneID" id="20564700"/>
    <dbReference type="KEGG" id="ero:EROM_111010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111010"/>
    <dbReference type="HOGENOM" id="CLU_1562864_0_0_1"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045103">
        <property type="entry name" value="RNF5/RNF185-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12313">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="49"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN84083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="178" mass="19964" checksum="1D72781E81E6058A" modified="2012-10-03" version="1">MSKGNRYPMKTKKETVEPDGWGKEATKTSTCIENANEKKSSHQSREYTCNICYSQPEGPVLTPCGHLFCWGCLYVWSQSTGGCKFCPTCRSRMEIEEVISVLAVDSKKESRGLPPRPMNNRKLVRVITPGIKVNGTRFGSCLLQEEEANIFSYRTTIGLLTLMCLLISITLKSYSFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLG2</accession>
    <name>I6ZLG2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetylglucosamine diphosphorylase</fullName>
            <ecNumber evidence="3">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_111670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="3"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265645.1">
        <property type="nucleotide sequence ID" value="XM_009267370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLG2"/>
    <dbReference type="GeneID" id="20564766"/>
    <dbReference type="KEGG" id="ero:EROM_111670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111670"/>
    <dbReference type="HOGENOM" id="CLU_025603_1_2_1"/>
    <dbReference type="OrthoDB" id="532420at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039741">
        <property type="entry name" value="UDP-sugar_pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952:SF2">
        <property type="entry name" value="LD24639P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11952">
        <property type="entry name" value="UDP- GLUCOSE PYROPHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="335" mass="37537" checksum="0F0C104EC85BCC86" modified="2012-10-03" version="1">MGYINMNSNNLIKPYEGSELNDLGRRYKKVGEEALRKKSLGVVILSGGQGTRLGSDAPKGLFKIKGKTLFEWHMETIREIIDKYNANITVFIMTSSFTDEAVRNYFQGKDFGVKIHFFKQRNSLCVGTDGKPLEYYGGYAESPYGNGDIFKAIQQVNLEGIEALNVISIDNVLAKILDPVFVGAFFSGNYDILSKSVTKGEKESVGAFLMDGKLRIKEYGENDANGEGIQGNICNHMFGTSFIKKMRNVDLPEHKAFKKIAYTIDGKLIKPLKPNGFKKETFIFDSFEYTHKNGVINVPREKEFSPLKNGMDSSVDNPMTCALAVERHRIQTSIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS86</accession>
    <name>I6ZS86_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerophosphoryldiester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_011220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263963.1">
        <property type="nucleotide sequence ID" value="XM_009265688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS86"/>
    <dbReference type="GeneID" id="20520749"/>
    <dbReference type="KEGG" id="ero:EROM_011220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011220"/>
    <dbReference type="HOGENOM" id="CLU_636203_0_0_1"/>
    <dbReference type="OrthoDB" id="197419at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051578">
        <property type="entry name" value="GDPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958:SF1">
        <property type="entry name" value="GLYCEROPHOSPHOCHOLINE PHOSPHODIESTERASE GPCPD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22958">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="164"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="431" mass="49880" checksum="387297372EEB9FBA" modified="2012-10-03" version="1">MKQIDWSNPEFFFKNKLIENSTTVEEEFRKIKVHRLERNKRILAPMIFCLVVKSTRAYRQMHIRVTLKKHMVEMEARTEIYPESFLMVEYSKEVGHREPFSVNVEAWSPKGMVSGKSFVVSDLELPSKTLVSLKIDYEGVMEEIEMEAHCVRTDQSISQIHEDMKIIGHRGCGTNGHPSTRMMENTVSSFKEAYRRGLKWVEMDVQLTRDEVPVIFHDFVIQSGASRIGINEITLKEFLHLVGNQDEENHSLPCTLSRFLEQIDDEHGVNIEIKYPLPSEEGRYKLKDLVPAERVVEKVVEMVQRSRKQRVIFSSFHPYILLMVKLRLPNFNIFMLTEAKGGEGNPYTNTLYDALYFCTKLGLDGVVLDWSCMAINPIDIVKIFKSFDLKTFVYGDEINDKNIISILKEAGAHGIIIDDLELICTQNNEHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS91</accession>
    <name>I6ZS91_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">thymidylate synthase</fullName>
            <ecNumber evidence="3">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_011270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dUMP + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="3"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263968.1">
        <property type="nucleotide sequence ID" value="XM_009265693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS91"/>
    <dbReference type="GeneID" id="20520754"/>
    <dbReference type="KEGG" id="ero:EROM_011270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011270"/>
    <dbReference type="HOGENOM" id="CLU_021669_0_2_1"/>
    <dbReference type="OrthoDB" id="766at2759"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.572.10:FF:000013">
        <property type="entry name" value="Thymidylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002497">
        <property type="entry name" value="PRK01827.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF2">
        <property type="entry name" value="THYMIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="9">
        <location>
            <begin position="11"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="294" mass="33461" checksum="BB525FB7F145AF33" modified="2012-10-03" version="1">MSQESKHAEYQYLDLVQHILENGVKRADRTGTGTLSVFGATMRFSLEDNTFPLLTTKRVFYKGVVEELLFFLRGQTDSKILEEKGIRIWEKNGSKQFLQSIGIDREEGDLGPIYGFQWRHFGAEYRTSGSSYEGKGVDQIKDVIDTIKKNPNSRRMVVCAWNPTALGEMALPPCHVLFQFNVMDGKLNCAMYQRSGDMGLGVPFNIASYSLLTIIVAHLTGLQPGEFVHFLGDTHIYLDHVDSLRLQLQRAPRPFPKLFVNPKEKRGKVEDFQYEDFSLVGYDPHPPIKMDMSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSV6</accession>
    <name>I6ZSV6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264163.1">
        <property type="nucleotide sequence ID" value="XM_009265888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSV6"/>
    <dbReference type="GeneID" id="20520956"/>
    <dbReference type="KEGG" id="ero:EROM_030450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030450"/>
    <dbReference type="HOGENOM" id="CLU_018687_0_1_1"/>
    <dbReference type="OrthoDB" id="27140at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF27">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="41"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="336" mass="39637" checksum="6C188854A0A879A7" modified="2012-10-03" version="1">MDFYKKRERTVLHSLISKQISPTDSTPLNVHEVKKYCYYGFSNASLRPKYWKVFLGYYSKNKFRTEMFLRNMRNSYSFYAGRVGNKFEGKEECYKVIENDVSRTFIKPRVDYEGPGEKERYCEFLDGTSENSEETHRDVIKRILKCYAMSNSSVRYVQGMNLVLIAIYYVLYLSDDEEDRKYCEEDSFFCFNSLMAEIGDNFIRDLDRCNGGIMHRMSVVMEIVKKADGELYGVMRRKGLTEEGFHMKWILLMFMSCFEIEDVIWLWDRLLSDTYRFEMVLYCCASAIIIMRNVIIQEDFDVCMELLQKPSVVGVETMFNVADHLRRKSSGTSKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSW4</accession>
    <name>I6ZSW4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264173.1">
        <property type="nucleotide sequence ID" value="XM_009265898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSW4"/>
    <dbReference type="GeneID" id="20520966"/>
    <dbReference type="KEGG" id="ero:EROM_030550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030550"/>
    <dbReference type="HOGENOM" id="CLU_072439_6_0_1"/>
    <dbReference type="OrthoDB" id="1677536at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044391">
        <property type="term" value="C:ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000018">
        <property type="entry name" value="40S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="136" mass="14656" checksum="8FC278425A1602EE" modified="2012-10-03" version="1">MTEQIETESMELSAVAHIKATKNDTFVHITDMTGSETIAKITGGMRVKAQRDEGSPYAAMLAAQDVAEKILGRGVKILHFKLRGAGGVKPMALGPGAQTAIRTLIRAGLKVGRIEDVTPVARDRVRKRGGHRGRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSZ9</accession>
    <name>I6ZSZ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoinositide polyphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_031040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264223.1">
        <property type="nucleotide sequence ID" value="XM_009265948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSZ9"/>
    <dbReference type="GeneID" id="20521017"/>
    <dbReference type="KEGG" id="ero:EROM_031040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031040"/>
    <dbReference type="HOGENOM" id="CLU_003016_7_3_1"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="474"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="2">
        <location>
            <begin position="139"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="529" mass="60526" checksum="405BB9D83A2F2A0D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT30</accession>
    <name>I6ZT30_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein AF-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">YAF9</name>
        <name evidence="5" type="ordered locus">EROM_031490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for an H2A variant leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex is also involved in DNA repair. Yaf9 may also be required for viability in conditions in which the structural integrity of the spindle is compromised.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SWR1 chromatin-remodeling complex and of the NuA4 histone acetyltransferase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The coiled-coil domain is required for assembly into the NuA4 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAF9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264268.1">
        <property type="nucleotide sequence ID" value="XM_009265993.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT30"/>
    <dbReference type="GeneID" id="20521063"/>
    <dbReference type="KEGG" id="ero:EROM_031490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031490"/>
    <dbReference type="HOGENOM" id="CLU_051385_3_1_1"/>
    <dbReference type="OrthoDB" id="16041at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000812">
        <property type="term" value="C:Swr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16887">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1970">
        <property type="entry name" value="YEATS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038704">
        <property type="entry name" value="YEAST_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005033">
        <property type="entry name" value="YEATS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055129">
        <property type="entry name" value="YEATS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23195">
        <property type="entry name" value="YEATS DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03366">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51037">
        <property type="entry name" value="YEATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="YEATS" evidence="4">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU367117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="151" mass="17492" checksum="8A9A85B302EDAE00" modified="2012-10-03" version="1">MEGYKSCRVIIGNEARVIPEEERAIPELTHEWKAYVKAPPEIVKKVEFKLHESFSNRLVSKEHPFEVVERGWGEFSIQMKILLFNGEKITTSHFLQLHGDSDVIINERIDEIVFKGNGSESIALEEEEEAEYERIEAGLVSMLEKFKEINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT55</accession>
    <name>I6ZT55_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">26S proteasome regulatory subunit 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264303.1">
        <property type="nucleotide sequence ID" value="XM_009266028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT55"/>
    <dbReference type="GeneID" id="20521101"/>
    <dbReference type="KEGG" id="ero:EROM_040370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040370"/>
    <dbReference type="HOGENOM" id="CLU_027018_3_0_1"/>
    <dbReference type="OrthoDB" id="10256771at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024969">
        <property type="entry name" value="EIF3F/CSN6-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540:SF7">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10540">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13012">
        <property type="entry name" value="MitMem_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPN" evidence="1">
        <location>
            <begin position="6"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="260" mass="29448" checksum="23FB047D5DE8CF93" modified="2012-10-03" version="1">MSEASVIVHPLVLLSAVDHYKRKGTKRVAGILLGNEDGEIHITESFACIFEEDEDGWFIDTSYIRSMFELFYKVNHKLKIMGWYHTGPRMYENDLDITRSLSKFVESPFLTIINVHLGENDLPIQAFKLDEQDEFIHVGCSIEAEEAEEVGVEHLIRDIREEASGSIAAKINGIKESLLVYKGVLGEIRSYLEDVIKGHISPSQEIIDLCQEIINSIPKLEKPLDENLSDCYVSALAKTVIALNDLRRNRLENGIEMNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTF3</accession>
    <name>I6ZTF3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_050020</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264433.1">
        <property type="nucleotide sequence ID" value="XM_009266158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTF3"/>
    <dbReference type="GeneID" id="20521235"/>
    <dbReference type="KEGG" id="ero:EROM_050020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050020"/>
    <dbReference type="HOGENOM" id="CLU_339243_0_0_1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF15">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="10">
        <location>
            <begin position="7"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="414"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="316"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="74"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="813" mass="92121" checksum="D472367E4FE104D8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTJ9</accession>
    <name>I6ZTJ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aminoacid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_050580</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264488.1">
        <property type="nucleotide sequence ID" value="XM_009266213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTJ9"/>
    <dbReference type="GeneID" id="20521290"/>
    <dbReference type="KEGG" id="ero:EROM_050580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050580"/>
    <dbReference type="HOGENOM" id="CLU_009020_1_2_1"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF678">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="12"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="385" mass="43570" checksum="BF4DD919C8B5FD8F" modified="2012-10-03" version="1">MKKNELGRECRSIFINLIKTTIGSGILRYPFLFKMYGVWYTTALTLISALASLLGLWLYIDLNDFYGLDNSMSTIARYIYPSLQPVVNVTVSVKCFLVCTAYLVLLKNNLPGTIKSIFGLEDTHQALCMAGVVLCVFPFVSMHKIDKLRYTSSFGFGAAIALVLLSIYRYVVCKEVPTYGEGRVGNYLGELGSFVFGFTCHQNIFSVQNEMKVSDKAALKITVLLVLLMASLIYITFGLTNYLVLGDGMSENFFENIPEGMKNIIAMFYFLVVMMSIPLQTHPCRTYFLDLFDTRYSADGKYWHLRFVTSLFIILLSFMIAMNVSKMERLCEIIGGSFSTLMCFVFASLYYFIAFWNRGFEMKKITALFVLLYGVAAFVSLFLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTW8</accession>
    <name>I6ZTW8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">White ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_060210</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264613.1">
        <property type="nucleotide sequence ID" value="XM_009266338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTW8"/>
    <dbReference type="GeneID" id="20521418"/>
    <dbReference type="KEGG" id="ero:EROM_060210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060210"/>
    <dbReference type="HOGENOM" id="CLU_020421_0_0_1"/>
    <dbReference type="OrthoDB" id="2196280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF89">
        <property type="entry name" value="FI03229P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="416"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="645"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="22"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="688" mass="77985" checksum="ACE9FD9EFF8CD61F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU58</accession>
    <name>I6ZU58_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_060890</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="10"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264678.1">
        <property type="nucleotide sequence ID" value="XM_009266403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU58"/>
    <dbReference type="GeneID" id="20521485"/>
    <dbReference type="KEGG" id="ero:EROM_060890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060890"/>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_1"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF8">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="14">
        <location>
            <begin position="7"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="15">
        <location>
            <begin position="258"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="381" mass="41005" checksum="2B32AF5F2F0E274A" modified="2012-10-03" version="1">MTSGEEVVIVCALRTPIGRAGKGKLKSLKNDELITAAIQGVIEKTRIDPKLIEEVVLGHCLSSMEGSVAARIGALRAGIPAETPVMTINRLCASGMESIGLIAEKIKSGRIEIGLAGGFESMSSYGLPQEYNLSRNETSTDARDCFLSFGEVTEMLSKTYGITRDEADEYASMSQRRALEAMKKGNLLKEIIQMKVGDENIKDDEGIRETLLEVIKKLKPVFRDDGICTSANSSQLSDGASAILLMKRRKADELGLPVIGEFVDFIVVGLKPRDMGLGPVVAIEKLLDRNNLKKDQISYFEINEAFASQVLCCLRRLGIGEDKVNRYGGAIALGHPIGASGARIVCTLLNVMENESLGEYGVASLCVGAGHGIAALFKKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU68</accession>
    <name>I6ZU68_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sulfhydryl oxidase</fullName>
            <ecNumber evidence="6">1.8.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061040</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2</text>
            <dbReference type="Rhea" id="RHEA:17357"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16520"/>
            <dbReference type="ChEBI" id="CHEBI:17412"/>
            <dbReference type="EC" id="1.8.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.8.3.2" evidence="6"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264693.1">
        <property type="nucleotide sequence ID" value="XM_009266418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU68"/>
    <dbReference type="GeneID" id="20521500"/>
    <dbReference type="KEGG" id="ero:EROM_061040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061040"/>
    <dbReference type="HOGENOM" id="CLU_070631_2_2_1"/>
    <dbReference type="OrthoDB" id="59470at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016971">
        <property type="term" value="F:flavin-dependent sulfhydryl oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.310:FF:000002">
        <property type="entry name" value="Sulfhydryl oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.310">
        <property type="entry name" value="ERV/ALR sulfhydryl oxidase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039799">
        <property type="entry name" value="ALR/ERV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036774">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017905">
        <property type="entry name" value="ERV/ALR_sulphydryl_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645">
        <property type="entry name" value="ALR/ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12645:SF1">
        <property type="entry name" value="FAD-LINKED SULFHYDRYL OXIDASE ERV2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04777">
        <property type="entry name" value="Evr1_Alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69000">
        <property type="entry name" value="FAD-dependent thiol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51324">
        <property type="entry name" value="ERV_ALR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERV/ALR sulfhydryl oxidase" evidence="7">
        <location>
            <begin position="57"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU371123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="164" mass="19073" checksum="2E53363FC8BE8C1E" modified="2012-10-03" version="1">MKKEQSYGRILIILVAAWVLYRCYGLIVRSREEKISTAFLRDTTMDGVENFQTRKMSKKEIRERLGRSTWTLLHTMGSRYPAFPTFQQKKDTLTFIHLLSSVFPCGDCTKHFQKLLRDHPPRVGSNEEFKAWLCEVHNVVNERLGKAVVDCKTVDNIWQCGCEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUT4</accession>
    <name>I6ZUT4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264978.1">
        <property type="nucleotide sequence ID" value="XM_009266703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUT4"/>
    <dbReference type="GeneID" id="20521797"/>
    <dbReference type="KEGG" id="ero:EROM_080610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080610"/>
    <dbReference type="HOGENOM" id="CLU_083680_0_0_1"/>
    <dbReference type="OrthoDB" id="2189795at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="289" mass="33248" checksum="4C8A221FDC6CADE5" modified="2012-10-03" version="1">MKVQKTIELIKRSYGQPILFHRLHCHLAYILGKSNPLHEVLDDWSRMLIFSATRNRGQNQGLEGKILSFLKEIRPPMNDKETRLKLWIVLYYMRSRSPSQANHLVIFELVSNFMGDSAFVDGLILSILCGVITCSNFGLERNKKLRNDTIVYLLEVIKGKSLDGLNRAIALPCYIDHGIEPPSLRDLSIGNDVQTLIVLENVCFYAKYSKSVEFVKRIVPDKVSFVDCLKRFISRSFCVDKREDSECTIADGVIESFSILDDIRKAYKEARDKKKFVSKIIEFTMELDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUV5</accession>
    <name>I6ZUV5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Inner centromere protein ARK-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265013.1">
        <property type="nucleotide sequence ID" value="XM_009266738.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUV5"/>
    <dbReference type="GeneID" id="20521835"/>
    <dbReference type="KEGG" id="ero:EROM_081010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081010"/>
    <dbReference type="HOGENOM" id="CLU_750117_0_0_1"/>
    <dbReference type="OrthoDB" id="2193638at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="369" mass="41410" checksum="B52234E23C0793C2" modified="2012-10-03" version="1">MELLKEFLEEWRSAVGRVKDRVICEQIGIRDECSAVVSEKAGACPKEVSGEVDKENDERFPCDGDFEGSVFDHESLIPGHIPIIKNRDEKDLSMQEERRDVVGPQAMSSCMDMSRENNSNCKELIAKEETNGVDLYSSESCKGVKLSKPSVIRDLAINPPKRRDVLEPSARDAKSEKEVGKVQLGVMSKDKDGKNLSKNVKLNEALIQLKKRYLDRVNESVCGKAEKGMRIEEMSKPGVAGSHKVGSPPFNANGQESEAMKRFDGSNLYKQAIDHQLLKSAGRVPQDVYKPKTKIPRVDEDDIILDPDFIVPDFAKDPAINFKVKMQDHRALEKYFSNGHEIDVERLFPHVRNVSNNSPNKWPSKRSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV42</accession>
    <name>I6ZV42_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_082020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0328 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265113.1">
        <property type="nucleotide sequence ID" value="XM_009266838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV42"/>
    <dbReference type="GeneID" id="20521937"/>
    <dbReference type="KEGG" id="ero:EROM_082020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_082020"/>
    <dbReference type="HOGENOM" id="CLU_059413_0_0_1"/>
    <dbReference type="OrthoDB" id="2195091at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019081">
        <property type="entry name" value="UPF0328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09591">
        <property type="entry name" value="DUF2463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="257" mass="29565" checksum="BF579FF42CE4B9FB" modified="2012-10-03" version="1">MNILGLYKIRESSEKKNKPSQSQYKGFLAFISMALPMLIYYIFKEHEFDGNPLLKFLTVLPPLSYSAVEYFTLFRDNWYSDWKSLTILQRMFYFIFNILFAMFSIVSIFSAIIFPFAEWDDNDGVLANSVFLPSLFVPLPYLLCTSCSLTPELISFIDTGTTICIDLVILSLSIVSLILLSKGPEHYIYIPTSSLIFILVRSLKEYIFPSSKYLEYIITWRTLIFIVIFLTNVFIYVSVGLSISTPSRIEAFLGSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVL4</accession>
    <name>I6ZVL4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2415 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265278.1">
        <property type="nucleotide sequence ID" value="XM_009267003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVL4"/>
    <dbReference type="GeneID" id="20564389"/>
    <dbReference type="KEGG" id="ero:EROM_091650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091650"/>
    <dbReference type="HOGENOM" id="CLU_047613_0_0_1"/>
    <dbReference type="OrthoDB" id="64353at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019417">
        <property type="entry name" value="DUF2415"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991:SF9">
        <property type="entry name" value="DUF2415 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43991">
        <property type="entry name" value="WD REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_8G05640)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10313">
        <property type="entry name" value="DUF2415"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="184"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2415" evidence="2">
        <location>
            <begin position="236"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="319" mass="36047" checksum="3C6BE3BE955EC1F1" modified="2012-10-03" version="1">MSQEAREVSNLVGSIQKTCDFGITSQHWQLKDMIKIKGESLVFPRACNVVSYSPRENTTKTIYEGVDFFPTSICLKDDFLAVGGSRGHLKIKNLATNEVLSQVVSSSINNNICMNNNRIFICNNDRTLKIFTMELQNSGCINHVAQVNSCGISPDGKFLVVVGDSNDVFLYSIDNDSYKLVKKLKSTEDGGFSVSWNSMSSTFAVGTQDGYVCIWDIRSDGMLHRFESKQKDSHRGAVRNVKFSLKNSLDLLFFTEHFSFLTVCDMRDFRRCQRVKVFPDKQITGAIFSEMNDRIFVSTEDQIIDMNINSKSRRMFAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVR0</accession>
    <name>I6ZVR0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CCAAT binding transcription factor subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_100130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NFYB/HAP3 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265328.1">
        <property type="nucleotide sequence ID" value="XM_009267053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVR0"/>
    <dbReference type="GeneID" id="20564443"/>
    <dbReference type="KEGG" id="ero:EROM_100130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100130"/>
    <dbReference type="HOGENOM" id="CLU_066247_12_3_1"/>
    <dbReference type="OrthoDB" id="386949at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016602">
        <property type="term" value="C:CCAAT-binding factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22907">
        <property type="entry name" value="HFD_NFYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027113">
        <property type="entry name" value="Transc_fact_NFYB/HAP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064">
        <property type="entry name" value="CCAAT-BINDING TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11064:SF9">
        <property type="entry name" value="NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="5">
        <location>
            <begin position="14"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="118" mass="13689" checksum="5D449C445702395B" modified="2012-10-03" version="1">MDEFSEGLRPTDRSLPIANISKIMKKPIPKEAKVAKDAKEIMQKSAGEFIAIITCRAKEICESEARKTVTGEDLIRAMDELDMPYYAELARKYYIQYRELAKNERVRKYSRGFDYGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVT3</accession>
    <name>I6ZVT3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Uracil-DNA glycosylase</fullName>
            <shortName evidence="5">UDG</shortName>
            <ecNumber evidence="5 7">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">UNG1</name>
        <name evidence="10" type="ordered locus">EROM_100400</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="5 7"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265353.1">
        <property type="nucleotide sequence ID" value="XM_009267078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVT3"/>
    <dbReference type="GeneID" id="20564470"/>
    <dbReference type="KEGG" id="ero:EROM_100400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100400"/>
    <dbReference type="HOGENOM" id="CLU_032162_3_0_1"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003588">
        <property type="entry name" value="PRK05254.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003589">
        <property type="entry name" value="PRK05254.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="9">
        <location>
            <begin position="131"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="303" mass="34670" checksum="6E619ED79DB9DBBE" modified="2012-10-03" version="1">MMEQEKEERHIYTNKSSKNMKEHKEDRSGGASSHPDSRNGLGKRQMSLDDIFSKKKIATVVIPSKIQETDWICEGCVNPCEYCKLEEKIDLRWRGYLKSEFGKDYFRGIKRFLHGNRNHLPPIDKIFTFSSFFPLEATKVVIMGQDPYHNDNQAMGLSFSVPMGVPIPPSLKNIYLEISSDIEGFEIPSHGDLSMWAKRGVLLLNDVLTVTKNRPNSHAGIGWREFTSRILQTINERCTNVVFMLWGRHAQAKGKFISRSKHLVLACGHPSPLSSNHFFGCKHFSKANKYLMDHGKSPIDWQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW86</accession>
    <name>I6ZW86_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265523.1">
        <property type="nucleotide sequence ID" value="XM_009267248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW86"/>
    <dbReference type="GeneID" id="20564643"/>
    <dbReference type="KEGG" id="ero:EROM_110440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110440"/>
    <dbReference type="HOGENOM" id="CLU_165637_0_0_1"/>
    <dbReference type="OrthoDB" id="2190056at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="13689" checksum="C0670A8D1F1AB3FF" modified="2012-10-03" version="1">MLFFLLASIVADNVEISRRSFDTLKVGNAYISSSPDSASTAWNSVKISFPGYHRYDIRENEPRKFELIDLTQYFGEKEEMQFRIDFETKYYGLIPDAWAMIAALTISSVVMFIIPLKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWK3</accession>
    <name>I6ZWK3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Importin subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_111650</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the importin alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265643.1">
        <property type="nucleotide sequence ID" value="XM_009267368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWK3"/>
    <dbReference type="GeneID" id="20564764"/>
    <dbReference type="KEGG" id="ero:EROM_111650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111650"/>
    <dbReference type="HOGENOM" id="CLU_018084_6_0_1"/>
    <dbReference type="OrthoDB" id="29145at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.690">
        <property type="entry name" value="Importin-alpha, importin-beta-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002652">
        <property type="entry name" value="Importin-a_IBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036975">
        <property type="entry name" value="Importin-a_IBB_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024931">
        <property type="entry name" value="Importin_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23316">
        <property type="entry name" value="IMPORTIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01749">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005673">
        <property type="entry name" value="Importin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51214">
        <property type="entry name" value="IBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="IBB" evidence="9">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="6">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="537" mass="60455" checksum="338D151BD9EFCA23" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE35</accession>
    <name>I7AE35_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DM2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264382.1">
        <property type="nucleotide sequence ID" value="XM_009266107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE35"/>
    <dbReference type="GeneID" id="20521182"/>
    <dbReference type="KEGG" id="ero:EROM_041190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041190"/>
    <dbReference type="HOGENOM" id="CLU_069185_0_0_1"/>
    <dbReference type="OrthoDB" id="10263741at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="CDD" id="cd10568">
        <property type="entry name" value="SWIB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.245.10">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036885">
        <property type="entry name" value="SWIB_MDM2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003121">
        <property type="entry name" value="SWIB_MDM2_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02201">
        <property type="entry name" value="SWIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47592">
        <property type="entry name" value="SWIB/MDM2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51925">
        <property type="entry name" value="SWIB_MDM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DM2" evidence="2">
        <location>
            <begin position="166"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="350" mass="40609" checksum="FAD51A1D4D8074FB" modified="2012-10-03" version="1">MTTLYDKLQSIEEEVDRLCLARKLNMEAEYLKRIKCKKYLRCYVQVAIKKGTFIRLDSRVINDYKNGGEMKFSDVVKRFCVIFDSNLATTTETHFGGRKASSEDGEGTEHPGIVNGDGNLPKSVCNGGISMEGFFEWTKCCGDTEAFEINSDKKPKNIKLLFDLENPREIFRLSTKLSNLLMKYTDTKPNVVTHLWRYVNRNGLMSIDSDIVECDLPLKDILGVERFEFPQLPDLIVPHLWPLDYLVVDIPSVDGHTEIFDIPFEWDDLYQCPTLYTKRIYALDRKIEALKHLLKRCENREDVLSEFEKDPAAFINKWICIDTSDVCHRTSLFRNKDMQEKIFGLLKKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEC4</accession>
    <name>I7AEC4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">U2 snRNP auxiliary splicing factor U2AF large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_050730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264502.1">
        <property type="nucleotide sequence ID" value="XM_009266227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEC4"/>
    <dbReference type="GeneID" id="20521305"/>
    <dbReference type="KEGG" id="ero:EROM_050730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050730"/>
    <dbReference type="HOGENOM" id="CLU_863558_0_0_1"/>
    <dbReference type="OrthoDB" id="10266058at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23139">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="356" mass="40763" checksum="870F8149E4B8D88F" modified="2012-10-03" version="1">MGVLPLNKRFRRMNLFDVGRCIIEGVEYVEEVLESNICITKEYEKDLYVPLLLPRLRTDEFTEEDYRNNVTIVCLTSLSLSSLDGFLLRFFRGFTLKKTDTGAEVEFIERQDATECLYLSNSDFTFLRPIGYIELPRAPEARNKGSKYCRDVPCTRDKILLGPLNIDSNLLRDALDEISPLQSFRVCRNPLYFVFTFRNSEFCEPFVKATSHIFISDAGRSLVSIRAYKGCSILNLGKNIPHLVPRRMTGPIALSKERTRIVTVLNVLGPWDLDVSKIVEEIKSRCSKYKGVKDVMVPRDSLRSGRQPGSSRVFIECKDLETSEKVYDELGGLIYKDRIVATGYYPELNYAAGEYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEF4</accession>
    <name>I7AEF4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_051140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264542.1">
        <property type="nucleotide sequence ID" value="XM_009266267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEF4"/>
    <dbReference type="GeneID" id="20521346"/>
    <dbReference type="KEGG" id="ero:EROM_051140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051140"/>
    <dbReference type="HOGENOM" id="CLU_472590_0_0_1"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA2/NAM7 helicase helicase" evidence="6">
        <location>
            <begin position="169"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="DNA2/NAM7 helicase-like C-terminal" evidence="7">
        <location>
            <begin position="361"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="571" mass="65724" checksum="BD3F857444EEED7E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEI7</accession>
    <name>I7AEI7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_051550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264582.1">
        <property type="nucleotide sequence ID" value="XM_009266307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEI7"/>
    <dbReference type="GeneID" id="20521387"/>
    <dbReference type="KEGG" id="ero:EROM_051550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051550"/>
    <dbReference type="HOGENOM" id="CLU_1669927_0_0_1"/>
    <dbReference type="OrthoDB" id="2192040at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="163" mass="19085" checksum="CAB11A58ACE02481" modified="2012-10-03" version="1">MEQKFYGDDIKSVNDDLNEARSSMQNEILDIKSDLLSVYLMNLVIYKEMNEEDRAGSSVEEMLAKTSILLEKICYMENKALALAKMPPNDEHKSCERKEDSEECGKRIITDKMKKNKFVKKRNIEDRIPRLKYKNKARKLSEMAEVKHDGNIDIKRPSFNKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEK1</accession>
    <name>I7AEK1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cyclosome/anaphase-promoting complexprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_060050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264597.1">
        <property type="nucleotide sequence ID" value="XM_009266322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEK1"/>
    <dbReference type="GeneID" id="20521402"/>
    <dbReference type="KEGG" id="ero:EROM_060050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060050"/>
    <dbReference type="HOGENOM" id="CLU_594506_0_0_1"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="226"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="122"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="460" mass="53807" checksum="A0F882D59AE543A4" modified="2012-10-03" version="1">MERLCSLISAYLIEEMDCSALALSEYVYREKKTDATIMLLLKSMYKLSCYERCIETIELNSSVMRIHEARIIYYRCKLLTGKKNKGLLTTMECDEEVEKYPPTEISKRSVRLLFEGLTKEDVKKKEILLEAYRSDNNNLEALLRMKNDSLINKKELLMLMDECEDKFMQNVYMEIFYPSFETDFYFLPFYSPWYGVKLAKQYYKSGKDMLLFNLGTTMVKLYPNSEHSFVALGLFFLMSSNYSEARRCFYKAVQINNDYGRGWLYLGMAYSGIKECESSIACLNIAYKVMIGSYKPAFYLAKEYHRMNNFERASFFYKHALGINSSVKVQERYISLLIYYEYYPEALSYLAAQKNSLLGLLRVYCNLFLGKVTEAQKYLSSCQTDWRYYATAGFIDHLLNNLDSAADNYNKALLKTHVNLVEELLGLAVENMTCKQNNNVYDYATDLFDNMIPKYSFETI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF14</accession>
    <name>I7AF14_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA 3'-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264757.1">
        <property type="nucleotide sequence ID" value="XM_009266482.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF14"/>
    <dbReference type="GeneID" id="20521567"/>
    <dbReference type="KEGG" id="ero:EROM_070090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070090"/>
    <dbReference type="HOGENOM" id="CLU_2885769_0_0_1"/>
    <dbReference type="OrthoDB" id="19045at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013954">
        <property type="entry name" value="PNK3P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08645">
        <property type="entry name" value="PNK3P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="63" mass="7350" checksum="80BE95B05639A2DD" modified="2012-10-03" version="1">MASDQSQVIRLNTLEVFVKKICNILVDLDVPIMLLAALKKNKYRKPSTGIYEYTCREYVDRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF31</accession>
    <name>I7AF31_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0235 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264777.1">
        <property type="nucleotide sequence ID" value="XM_009266502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF31"/>
    <dbReference type="GeneID" id="20521588"/>
    <dbReference type="KEGG" id="ero:EROM_070290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070290"/>
    <dbReference type="HOGENOM" id="CLU_130694_5_2_1"/>
    <dbReference type="OrthoDB" id="244097at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00251">
        <property type="entry name" value="DUF167 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420">
        <property type="entry name" value="UPF0235 PROTEIN C15ORF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420:SF7">
        <property type="entry name" value="UPF0235 PROTEIN C15ORF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="100" mass="11185" checksum="D2D64EBE52EC2D39" modified="2012-10-03" version="1">MAWYCIQDKKLRLSVRARPSARSTGIREISGTEIVVNVNAPPVENKANKELVRFISKMLRVPKSSIKIVIGTTSMQKTVEVSPWEGSIENLEATAKKMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFE8</accession>
    <name>I7AFE8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adaptin small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_080070</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264927.1">
        <property type="nucleotide sequence ID" value="XM_009266652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFE8"/>
    <dbReference type="GeneID" id="20521743"/>
    <dbReference type="KEGG" id="ero:EROM_080070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080070"/>
    <dbReference type="HOGENOM" id="CLU_2333602_0_0_1"/>
    <dbReference type="OrthoDB" id="2186857at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="6">
        <location>
            <begin position="6"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="98" mass="11664" checksum="055E50595695116A" modified="2012-10-03" version="1">MVLAQKERNIIDGNETIVFNKFGNLFIAFVVENENEMYVLSLINNLVSIYDRFFTKVCELHFIYNFKETHIILDNYIVNGKCIENDPFEVMKHAYYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFI6</accession>
    <name>I7AFI6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein RER1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_080660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RER1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264982.1">
        <property type="nucleotide sequence ID" value="XM_009266707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFI6"/>
    <dbReference type="GeneID" id="20521802"/>
    <dbReference type="KEGG" id="ero:EROM_080660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080660"/>
    <dbReference type="HOGENOM" id="CLU_074889_2_1_1"/>
    <dbReference type="OrthoDB" id="448250at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004932">
        <property type="entry name" value="Rer1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10743:SF0">
        <property type="entry name" value="PROTEIN RER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03248">
        <property type="entry name" value="Rer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016013">
        <property type="entry name" value="AtER_Rer1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="166" mass="19782" checksum="D507E6B754288E6F" modified="2012-10-03" version="1">MDLKTLKQIYLDRLAPRPDVRWGITGAMFVLYCVRIWTTGAFYLITYCLGIYLLHALILFLTPKGEMIPDPFENIEDDDYIPETIDNEFKPFIRNLPEFDFWMFVTKIVGMAFIGTYFDILDIPVYTPILVIYFIFMVGYTTKRLIAHMKKYNYNPFLQSKEYYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG53</accession>
    <name>I7AG53_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265222.1">
        <property type="nucleotide sequence ID" value="XM_009266947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG53"/>
    <dbReference type="GeneID" id="20564333"/>
    <dbReference type="KEGG" id="ero:EROM_091090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091090"/>
    <dbReference type="HOGENOM" id="CLU_101028_1_0_1"/>
    <dbReference type="OrthoDB" id="21221at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070646">
        <property type="term" value="P:protein modification by small protein removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
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        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
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        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
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        <property type="entry name" value="Peptidase_C97"/>
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        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="4">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="150" mass="17104" checksum="D58F4F821738764C" modified="2012-10-03" version="1">MEKHNVILRVYPLGDETMRRFVASSLGRKEVQIWHTSIEVYGMEYFFQNGITKALPGFTIHGTPVKTHDLGTTDIPEIVFEDFLLSIADDFAPHKYHLLRNNCNNFTNTVALYLVEKPIPEYILELQNTALESEAVSSIVDLFFGAPEQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGE0</accession>
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        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ordered locus">EROM_100120</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265327.1">
        <property type="nucleotide sequence ID" value="XM_009267052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGE0"/>
    <dbReference type="GeneID" id="20564442"/>
    <dbReference type="KEGG" id="ero:EROM_100120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100120"/>
    <dbReference type="HOGENOM" id="CLU_000524_5_2_1"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 23" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="22"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="183"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="440"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="539"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="621"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="678"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1050"/>
            <end position="1137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="AFN83830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1141" mass="128331" checksum="5539DE998F660C51" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGI4</accession>
    <name>I7AGI4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265377.1">
        <property type="nucleotide sequence ID" value="XM_009267102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGI4"/>
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    <dbReference type="KEGG" id="ero:EROM_100640"/>
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    <dbReference type="HOGENOM" id="CLU_105949_0_0_1"/>
    <dbReference type="OrthoDB" id="2186996at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="181" mass="20546" checksum="A1ACFFB12AB71BD9" modified="2012-10-03" version="1">MSTATPADIPQEYINNENPFAHMKEYSESQSLDPEVRLRLNALQQMQPKFIPLGINDRILKAERAVKTKAIVESQIEKLLDEGMKLIDSTVPEREEALRSNADDPRVLDILISISRLRLLSNQERLREKVYYALKSTILSDTITSIDYSSSIDEDHLSDAMEYGELVEKYSQTETPVSAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGU8</accession>
    <name>I7AGU8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265517.1">
        <property type="nucleotide sequence ID" value="XM_009267242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGU8"/>
    <dbReference type="GeneID" id="20564637"/>
    <dbReference type="KEGG" id="ero:EROM_110380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110380"/>
    <dbReference type="HOGENOM" id="CLU_849998_0_0_1"/>
    <dbReference type="OrthoDB" id="2192943at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="327" mass="37993" checksum="9F485428E0F0C669" modified="2012-10-03" version="1">MEVSRGKDSTKNSLNGTKPDINEEHLRPKETCFLEGNRPQVLEDTAGTESTKKEEASFRHPDSKRGLFEASASNHGVNKEETLTRDSDSSFDSDDTDRCSGLLSVYWIKEAEGLYKNLTSLRISDMALEKNRRNHPNPNFLCNTEERSNYTMVLEDIYFKQKTKNLKKEIKLKYLIEEFFMALDKFVLIANYCLKSTEIEDEDEDFNFALVWVRDAFHWLGIEGAKYYARKSPGPYNGWISDVEFLMNRYFFSPEFNHVNGFGIEQSMHEGYRFVESSDGKYYLSYQPYPGYYDVSEENKRFCTGCNLILPRSLSLPDDEYLNEEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGV8</accession>
    <name>I7AGV8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_110530</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="8"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265532.1">
        <property type="nucleotide sequence ID" value="XM_009267257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGV8"/>
    <dbReference type="GeneID" id="20564652"/>
    <dbReference type="KEGG" id="ero:EROM_110530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110530"/>
    <dbReference type="HOGENOM" id="CLU_004962_0_0_1"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07414">
        <property type="entry name" value="MPP_PP1_PPKL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000026">
        <property type="entry name" value="Serine/threonine-protein phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050341">
        <property type="entry name" value="PP1_catalytic_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031675">
        <property type="entry name" value="STPPase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668">
        <property type="entry name" value="SERINE/THREONINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11668:SF300">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16891">
        <property type="entry name" value="STPPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="9">
        <location>
            <begin position="120"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="317" mass="36238" checksum="E78A84A0DC23E7E1" modified="2012-10-03" version="1">MSNEKVDLDRIIEKLLSVRNSKLGRLVHLAEAEIRYLCEKSTEVFKQQPTLVEVKAPVKICGDVHGQYYDLLKLFEHGGFPPSSNYLFLGDYVDRGKQSLETICLLLAYKIKFPNNFFLLRGNHECASINRIYGFYDECKRRYDTTIWKLFTECFNWIPIAALVDGRILCMHGGISPDLKSMDQIKAIARPTDVPDEGLLCDLLWSDPDPSVRGWGENDRGVSVTFGPDTVERFLETHNLDLICRAHQVVEDGYEFFANRSLVTVFSAPNYCGEFENSGAIMDVDKDLVCSFQVLKPTDFINHTESRKKQMGMPRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGY5</accession>
    <name>I7AGY5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cyclin-dependent protein kinase 9-like protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_110830</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265562.1">
        <property type="nucleotide sequence ID" value="XM_009267287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGY5"/>
    <dbReference type="GeneID" id="20564682"/>
    <dbReference type="KEGG" id="ero:EROM_110830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110830"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_6_1"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="316"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN84065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="329" mass="37699" checksum="FC95BA73850F6CC7" modified="2012-10-03" version="1">MGLSEEGEIFEYRRFTITKMEYKKIRTIGEGTFGQVILAGKGRARYALKKVNKEKEGLSVTTIREIQALREMNHPSIIKLIEMVVEPNGDVYMVFPYFPYDLNRFIRSNRPTSDEIKHIFYQIVKGVHYIHSKGVMHRDLKSANILLDHRLNASIADFGMARHVTKSGMYTPGMVTLWYRPPEILLGSSSYTYSVDVWSLGCILTEMYLGHMIFQGSTEILQLEMIIHACGSINESTYPGVQNLPGFRNFRLPQSPRRIEAIIAKHDESAVGLVSRMLCLDPSKRITMDQIIENKYFDNEVFKDSSGTEFQDGCHRRGKPSPCKRKNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH09</accession>
    <name>I7AH09_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATM1 mitochondrial ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_111080</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265587.1">
        <property type="nucleotide sequence ID" value="XM_009267312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH09"/>
    <dbReference type="GeneID" id="20564707"/>
    <dbReference type="KEGG" id="ero:EROM_111080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111080"/>
    <dbReference type="HOGENOM" id="CLU_000604_84_1_1"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF654">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="34"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="342"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN84090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="578" mass="64450" checksum="19F7168C9471E06E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL66</accession>
    <name>I7AL66_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263891.1">
        <property type="nucleotide sequence ID" value="XM_009265616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL66"/>
    <dbReference type="GeneID" id="20520676"/>
    <dbReference type="KEGG" id="ero:EROM_010500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010500"/>
    <dbReference type="HOGENOM" id="CLU_1042164_0_0_1"/>
    <dbReference type="OrthoDB" id="2189578at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="31404" checksum="539AE93037C77666" modified="2012-10-03" version="1">MHNPEDIVKEALITVPSPRRFPSLEIASGLEVPKTMSPRSLEDLNELYREMEFPTIEERMLDDHMALLDVLNNLSIEYNRYGLIISSELDELRKKQEEIALYDEYERECDILEVELANMKQKYEETWKYSCHEISDLRHRLDELRKMFNDMEYGRLHSNLINRIFAFLREEGPGDEEVVSSLGYVKETREYLNSMEKMLVLDEIGNIPDSYNKIVCQHLIHEESIELDKLGKILGVDRTVLLRIVYGLLSKGIIAFDRSEDRICLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALF5</accession>
    <name>I7ALF5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264001.1">
        <property type="nucleotide sequence ID" value="XM_009265726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALF5"/>
    <dbReference type="GeneID" id="20520787"/>
    <dbReference type="KEGG" id="ero:EROM_020290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020290"/>
    <dbReference type="HOGENOM" id="CLU_818972_0_0_1"/>
    <dbReference type="OrthoDB" id="2192279at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="339" mass="39463" checksum="DD81CE3291351907" modified="2012-10-03" version="1">MNLQANKRTCIFLHTISTMITIFSMAYEKKSFLKNVQNEIEIRKLMIDQELYTELLSRIADGNYLVTQKGSKLFRYLKDTNILPGIYLVGSRGRQEHVSLEKVPYLDPRAKYFFIDIKDIPLYRMFLKNNLRSFIHTKGFDLDHTENEILQMSRISQECLRILDRIGSTKIVMEDMIVKLNKGNPDELCIEHSQGLFITKSLFNNRNEFLEDLKTHFVGFMDLIPFGMKTSSFCSAYAKSVINYLKRENVLLLSPSIKRYPELIHYLFPYRIISGTSNLSSNQLVVGGVSFNGIHQNDYLKELFDLEKIKKITENGVKFIFTAHVSIDSRKRKYGISFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALG2</accession>
    <name>I7ALG2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">26S proteasome regulatory subunit RPN2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_020390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the proteasome subunit S1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264011.1">
        <property type="nucleotide sequence ID" value="XM_009265736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALG2"/>
    <dbReference type="GeneID" id="20520797"/>
    <dbReference type="KEGG" id="ero:EROM_020390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020390"/>
    <dbReference type="HOGENOM" id="CLU_019837_0_0_1"/>
    <dbReference type="OrthoDB" id="2196194at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042176">
        <property type="term" value="P:regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016642">
        <property type="entry name" value="26S_Psome_Rpn2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002015">
        <property type="entry name" value="Proteasome/cyclosome_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10943:SF2">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01851">
        <property type="entry name" value="PC_rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015947">
        <property type="entry name" value="26S_Psome_Rpn2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR015947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="785" mass="87972" checksum="53D5D3876197F684" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALL8</accession>
    <name>I7ALL8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264066.1">
        <property type="nucleotide sequence ID" value="XM_009265791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALL8"/>
    <dbReference type="GeneID" id="20520855"/>
    <dbReference type="KEGG" id="ero:EROM_020940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020940"/>
    <dbReference type="HOGENOM" id="CLU_041164_0_0_1"/>
    <dbReference type="OrthoDB" id="2384430at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052945">
        <property type="entry name" value="Mitotic_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628">
        <property type="entry name" value="ACTIVATOR OF C KINASE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43628:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATORY FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707842" evidence="1">
        <location>
            <begin position="20"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="509" mass="58328" checksum="B8067655CC490FFB" modified="2012-10-03" version="1">MFILHLVFSLCTSLEKALLEQGDVENAIRLCGTKEGPLDYFYSYFVKKVFMGKSEEALLDIAGSDNYKDARNIVLGVMSMESGNTNERAKLGADLLWNVAVKVSRKYLRNPHAFLATDAFEKLEKNSLTKRGIGNIVTMVYSGDDISARLFLSLLDNRKIYIGDYIDVIEFLGRNGYGRALGYLGEIYYHGIGVKRSLDQAMYFFSKGKEMQDTVGACGVGKILMSSEYKDYKNAKSALSLANRLGQSGEAEYLMYLLMSRRPEYVHRAGPHMDSALMYGYLPAICRDGVNYYTNGQYINACIRFHPIMDYSDIVYDLRKRAEASFISRKYRQCVMALLMCVELGSVSSIRNAIYVLKRHYVFKRQEHLLFELYMKLLRKGDQDVINRIGDAYFYGSGVEKSYRDAFAFYISASLARDPEGCYNVSYMYEHGYGVKKSLLLAYKYILKIRSTDDMYLLLLYTHIRLFFKLIARLLLNRYSISICIGGLVVRRIYITKRAAPNEQETKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALV6</accession>
    <name>I7ALV6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">TatD deoxyribonuclease-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030100</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264126.1">
        <property type="nucleotide sequence ID" value="XM_009265851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALV6"/>
    <dbReference type="GeneID" id="20520917"/>
    <dbReference type="KEGG" id="ero:EROM_030100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030100"/>
    <dbReference type="HOGENOM" id="CLU_031506_1_1_1"/>
    <dbReference type="OrthoDB" id="6079689at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050891">
        <property type="entry name" value="TatD-type_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060:SF15">
        <property type="entry name" value="DEOXYRIBONUCLEASE TATDN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10060">
        <property type="entry name" value="TATD FAMILY DEOXYRIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="273" mass="30984" checksum="9889A64F600B07B7" modified="2012-10-03" version="1">MFIDIAVNITDRLLARDEGSVEEVIKRCKDSKVLPIFVGLDHNTSKRCVSLARKYKTVSTVGVHPTSSSRYKNIDEIIPLINDDVVVAIGECGLDYDRLEFADKTSQKRIFRSQLDLKGDCYFLHSRSCHRDFMETISDYEIRGVVHSFTGDIEEAKELIKKGLFIGVNGCSVKTPEGIDVIRSLPLESLLIETDSPYCKIRKSYAGFKYVTTDFGGEKVLKKKNEPCCIIQMAEIISSITERDYSYVVNTLFENTIRLYGDSVKRSVEEWGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMD2</accession>
    <name>I7AMD2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264356.1">
        <property type="nucleotide sequence ID" value="XM_009266081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMD2"/>
    <dbReference type="GeneID" id="20521156"/>
    <dbReference type="KEGG" id="ero:EROM_040920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040920"/>
    <dbReference type="HOGENOM" id="CLU_2306892_0_0_1"/>
    <dbReference type="OrthoDB" id="2190268at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="110" mass="12567" checksum="8C1DCF8FBB25B403" modified="2012-10-03" version="1">MIRLPGDGVDVLTYNDQPLMIAMFPEIGCKARARIVKVTFTQVIVQIFEVEKRKTTIEYKGIFRPADFNPNEWLCDKFGKDDVVECTILSYGDSGIFVNFDMKIVERPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMR9</accession>
    <name>I7AMR9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_051030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264531.1">
        <property type="nucleotide sequence ID" value="XM_009266256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMR9"/>
    <dbReference type="GeneID" id="20521335"/>
    <dbReference type="KEGG" id="ero:EROM_051030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051030"/>
    <dbReference type="HOGENOM" id="CLU_040729_10_5_1"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45732:SF7">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="30"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="207" mass="24931" checksum="5794E478D621B2D2" modified="2012-10-03" version="1">MHEICRRVKKKVLDVYNRIFVIRVRVAVLGPEKSGKSTIVNRMFKDRLRYLGSQKDMCLYKYDEDYINYVVYDVCGKRSSRVRWDYFYKRCDVILYCVDASSTEEKWVEAREELKSMLYRNPWIKRSILVLGTKNEKKSALGCMEIIRKLELFDINGLKVSCFSVSAKKNTNIECIMSWIRDQCYFLRWSWGSRTIRRWWRLGSLKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMT9</accession>
    <name>I7AMT9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gamma glutamyltranspeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264556.1">
        <property type="nucleotide sequence ID" value="XM_009266281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMT9"/>
    <dbReference type="MEROPS" id="T03.011"/>
    <dbReference type="GeneID" id="20521360"/>
    <dbReference type="KEGG" id="ero:EROM_051290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051290"/>
    <dbReference type="HOGENOM" id="CLU_014813_4_1_1"/>
    <dbReference type="OrthoDB" id="1081007at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686">
        <property type="entry name" value="GAMMA GLUTAMYL TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11686:SF9">
        <property type="entry name" value="RE13973P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="341"/>
            <end position="343"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="390"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR600101-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="511" mass="56593" checksum="04911E033F91A00C" modified="2012-10-03" version="1">MEGGNAMDAAIASTICIGIINSFSSGIGGGGFMLIKGPGTNGVLDMIDFRETAPRNLSDSGLSGMGGANEKGLKVGVPGEILGLYEAHKKYGKLPWRELFRENIMIARGFPASNLLVKKINKLKSYITSDPGLRDIYTRNGVLLKEGDIVARENYAKTLEIIMRHPESFYTGSLADLIVKAVRKKGGLIDKRDLEEYKVIHRDVIEGRYHDYKVYTTNLPTSGPLVLKALNIFEKYDLKKLKADSLRNGNFNHMHLLIEVFKFAMARRGELGDPAFLPGWEEIVKEIISKKSAEEAYRKINLGKVLDIKEYGMKTKGTEDHGTTHINVIDKDNMVVLLTSSINLEFGAKFMDPETGVVFNDTIDDFHISHARDVHSKHPNNVEGGKRSFSSMSPILLLKEDEILAIGAAGGIRIPTSVISTIFHLGTGENLREAIIETRIHNQLSPNITFVEHNIPKALERYLISIGHRIEKSLQNTIFTSVQGIFLKSVGGNRIIQAVSDPRKEGEAFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMW5</accession>
    <name>I7AMW5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Acylcoenzyme A thioesterase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_051590</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264586.1">
        <property type="nucleotide sequence ID" value="XM_009266311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMW5"/>
    <dbReference type="GeneID" id="20521391"/>
    <dbReference type="KEGG" id="ero:EROM_051590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051590"/>
    <dbReference type="HOGENOM" id="CLU_963455_0_0_1"/>
    <dbReference type="OrthoDB" id="68328at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049450">
        <property type="entry name" value="ACOT8-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20789">
        <property type="entry name" value="4HBT_3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="3">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA thioesterase-like C-terminal" evidence="4">
        <location>
            <begin position="192"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="290" mass="32399" checksum="13BB8A573E5B97FC" modified="2012-10-03" version="1">MEFFRITRLSDGVFEGANLWSPFPGYPAFGGQLAAQSLAAAFATVGENSIPITMSILFINRCKSSQKVKYAVKSLKDGSIVDMRQVDCYQNDVLVSSAHISFSRPDNNAHDYESNPYKIYDDTFIPFSEYISRSMATSSVPQAEVNAKFQILYDNMLMMLNVLDVDVGVENKDMRQIRIKIKEEQESIKGKASLLTLISDILLVETALMTSNLTLFSSDLSLLTSLNHVVNFVNLEKDISDGYLYYIVKCKGIRNSKAICEGQLIHEDGALICLTGQQGVFRVKSSYSRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN34</accession>
    <name>I7AN34_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nuclear pore complex protein Nup155</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_060400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the non-repetitive/WGA-negative nucleoporin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264631.1">
        <property type="nucleotide sequence ID" value="XM_009266356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN34"/>
    <dbReference type="GeneID" id="20521437"/>
    <dbReference type="KEGG" id="ero:EROM_060400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060400"/>
    <dbReference type="HOGENOM" id="CLU_014149_0_0_1"/>
    <dbReference type="OrthoDB" id="338970at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044611">
        <property type="term" value="C:nuclear pore inner ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036228">
        <property type="term" value="P:protein localization to nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1880">
        <property type="entry name" value="Nucleoporin, helical C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.440">
        <property type="entry name" value="Nucleoporin, helical domain, central subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007187">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014908">
        <property type="entry name" value="Nucleoporin_Nup133/Nup155_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004870">
        <property type="entry name" value="Nucleoporin_Nup155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042537">
        <property type="entry name" value="Nucleoporin_Nup155_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042538">
        <property type="entry name" value="Nucleoporin_Nup155_C_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10350:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03177">
        <property type="entry name" value="Nucleoporin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08801">
        <property type="entry name" value="Nucleoporin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like N-terminal" evidence="6">
        <location>
            <begin position="89"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoporin Nup133/Nup155-like C-terminal" evidence="5">
        <location>
            <begin position="717"/>
            <end position="860"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="964" mass="111562" checksum="3E9BCCB4B9EC9AFB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANA1</accession>
    <name>I7ANA1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264721.1">
        <property type="nucleotide sequence ID" value="XM_009266446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANA1"/>
    <dbReference type="GeneID" id="20521530"/>
    <dbReference type="KEGG" id="ero:EROM_061340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061340"/>
    <dbReference type="HOGENOM" id="CLU_151432_0_0_1"/>
    <dbReference type="OrthoDB" id="2188238at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="148" mass="16811" checksum="ABC1B14AB257700E" modified="2012-10-03" version="1">MGGMIYEGKGTDMEYEEVFDSLVKDLEEDVLSLQLTNFSDVNHTHETNGKCSMSVRDLDECTESVQVKNIEYNSQKYIFMRSINDDAQPSLIFISVDGDERGRKRGMLVTSFCGILKLIATFDYQAANSVAAKIFKVLEEYSCEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANM0</accession>
    <name>I7ANM0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264871.1">
        <property type="nucleotide sequence ID" value="XM_009266596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANM0"/>
    <dbReference type="GeneID" id="20521683"/>
    <dbReference type="KEGG" id="ero:EROM_071230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071230"/>
    <dbReference type="HOGENOM" id="CLU_098758_0_0_1"/>
    <dbReference type="OrthoDB" id="2190999at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="224" mass="25710" checksum="3F74EB12D9E1E52B" modified="2012-10-03" version="1">MRISLKDEKFFRGMLSIFNKKDDIKFEVKQGTISISSYGQSNFYLTMNDDVFMVNGDDLTFTVKAQHLLEGMDVLGKCDLAVEEEVRLIDGRNTIFIPFVTTMEGEYEEPDAPIAKLIVGSESLDALGVLKGIVTYEIEEDRLFIRKAGADLLEEIEFFAVDFITAGDMQFKCNNKWTDALERIKGYVDRIMLTFSLNALYAQFLFKRNPRSYLELRVLRSLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANU3</accession>
    <name>I7ANU3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264956.1">
        <property type="nucleotide sequence ID" value="XM_009266681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANU3"/>
    <dbReference type="GeneID" id="20521773"/>
    <dbReference type="KEGG" id="ero:EROM_080370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080370"/>
    <dbReference type="HOGENOM" id="CLU_165614_0_0_1"/>
    <dbReference type="OrthoDB" id="2191220at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="107" mass="12841" checksum="CBDAEC7324942197" modified="2012-10-03" version="1">MGKEEVNGRNKQRRYNEKETIKKFLVSSKGISFLVDHVNRESSNEDVRPNKKSIEKEVSNLIDYYFAWINGLPIRKNMTMSKYEFLKYLEDFCRKNDVSNLFEFLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANY0</accession>
    <name>I7ANY0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_080860</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265001.1">
        <property type="nucleotide sequence ID" value="XM_009266726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANY0"/>
    <dbReference type="GeneID" id="20521821"/>
    <dbReference type="KEGG" id="ero:EROM_080860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080860"/>
    <dbReference type="HOGENOM" id="CLU_001485_2_1_1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00106">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF29">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="3 4" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="6">
        <location>
            <begin position="30"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="448" mass="50905" checksum="7D3B2D241EB8BD54" modified="2012-10-03" version="1">MVWNGPTSSSMFFNKTPKPRLPIWKVMLDNVRTYLRIKPQAIPEDIVVGGDSIVVDGNKFSFDRIFRGSTQQEVFQEVSKSLLVECMQGYNCTLFAYGQTGSGKTYTIQGNAHNIGIVQRSLSLLHRNTDLKISLSFVEIYNEMMLDLFDPEASLSIREDPLCGVVVDNLSTVESDSYEKSMEMYSRGIRTRRTSSTCMNKESSRSHSIFIVYLKSNSGVMSKSSRLCFVDLAGSERLREREIEETKMKEAANINKSLLCLGKVINKLSNGEDGHIGYRESKLTFLLKDSLGGNCKLTVIGNVSLESRSDTVNTMNFLQRSKMIKNLAVVNSDVNGELEEVKNKLKALDSENQSLKARIALMDTQMQEESKVLPSYHYDIKRMKSVVDDTMNTLLELETVIKKIPNVEFDKSRRLLLSIHERFVSMYGREGEDGENSMKRKRMEEREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP33</accession>
    <name>I7AP33_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265061.1">
        <property type="nucleotide sequence ID" value="XM_009266786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP33"/>
    <dbReference type="GeneID" id="20521883"/>
    <dbReference type="KEGG" id="ero:EROM_081480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081480"/>
    <dbReference type="HOGENOM" id="CLU_752323_0_0_1"/>
    <dbReference type="OrthoDB" id="2195020at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="379" mass="43707" checksum="918289B873E4F9C7" modified="2012-10-03" version="1">MLLIYFVLICAKSWKEVGYIFSEGRQRFLTHNGVDIVVAKYSEKPLKFQMVKMRKDQSSNVLLYPIPGMGNKVADFNFSAHTNKRWYFYPEHGKWNQRTRFVLMPGNVLKIVIGDSCLGVDDADRVVGESCKDDQNDSLQIFSWVPITQKRKVKAWINQNRRRAHSDEYYPSLYEDERDFEKSHDQESSGYDGDENSSSDYHGRSGGAFHEFDYDSGIGGHGPFKSGIGSFGGRRRRYRRDEEMKHMGERDEESCNNDEGKDYREGRMFRKKRMGKLLRNGKARQHKPYGNREHDENFHQGRNPTFSDIGSPNFVNYLDNSQEYEQGAIEETPFVPCDDSLLMFGEDPGHCNVGGAGENEICKINKTTMAFRKMVGIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP38</accession>
    <name>I7AP38_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="2">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_081530</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265066.1">
        <property type="nucleotide sequence ID" value="XM_009266791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP38"/>
    <dbReference type="GeneID" id="20521888"/>
    <dbReference type="KEGG" id="ero:EROM_081530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081530"/>
    <dbReference type="HOGENOM" id="CLU_1885744_0_0_1"/>
    <dbReference type="OrthoDB" id="2195328at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pus10-like C-terminal" evidence="5">
        <location>
            <begin position="24"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="135" mass="15904" checksum="5E786A737A239907" modified="2012-10-03" version="1">MQRHDIPGRESVKQDIFCLSMLRKELDIQKGCVIDQRTPLRVLHRRANLAREKKTCILECERKDVDGWIYCRIKLEASAGTYIKEFVNGDFGRTTPSLGSKENYYDLLELDVLRVEKKSIRDFLTRRIKLDIIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP63</accession>
    <name>I7AP63_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265096.1">
        <property type="nucleotide sequence ID" value="XM_009266821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP63"/>
    <dbReference type="GeneID" id="20521918"/>
    <dbReference type="KEGG" id="ero:EROM_081830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081830"/>
    <dbReference type="HOGENOM" id="CLU_074702_1_1_1"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="1">
        <location>
            <begin position="52"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="199" mass="22626" checksum="F1A0E43609D0B995" modified="2012-10-03" version="1">MKIKELKMKLSKVKDFHKMSVGLEQYMTPPDIAASMVSVAHSTYDDIEGRSILDLCCGTGMLSFACGYFSPSYILGIDLCPSALETFRLNNLEFGINIDLVRCSIDDLTFIEGRFDTAIVNPPFGTKIKHADIKAVDKALEICNVVYSLHKTSTRKYITARYPNVEVLAKIKYELPRKHDFHKKDKKTIDVDFIRIQKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP80</accession>
    <name>I7AP80_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265116.1">
        <property type="nucleotide sequence ID" value="XM_009266841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP80"/>
    <dbReference type="GeneID" id="20521940"/>
    <dbReference type="KEGG" id="ero:EROM_090010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090010"/>
    <dbReference type="HOGENOM" id="CLU_1069694_0_0_1"/>
    <dbReference type="OrthoDB" id="2192264at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="29782" checksum="ABE7BE86561E39A2" modified="2012-10-03" version="1">MRLGIGGFGIICIINVVFSLVKKGRCVRLNYSGRNGKQVPREVFDSMMRGDRIIELNISGNKINVLPAYFENLSFMKVLDVSNNELKFLSSSIAGMPFLVSLNLNSNDLTFLPEEITELKYLENLSIRDNQLQELPEKMDDIASLVNLDLANNKLEDLPFGIGGVENLKRLDLEGNLFDTLPPILGYLENIEEIIFTKNRRLGEIPRKLVQMLANSKTLLLLDMRNNNRLREESTKDNVGWRELREIFGSKIRLSQDEEAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7APE8</accession>
    <name>I7APE8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265201.1">
        <property type="nucleotide sequence ID" value="XM_009266926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APE8"/>
    <dbReference type="GeneID" id="20564312"/>
    <dbReference type="KEGG" id="ero:EROM_090880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090880"/>
    <dbReference type="HOGENOM" id="CLU_087330_0_0_1"/>
    <dbReference type="OrthoDB" id="284782at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097361">
        <property type="term" value="C:cytosolic [4Fe-4S] assembly targeting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR PROTEIN ASSEMBLY PROTEIN CIAO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19920">
        <property type="entry name" value="WD40 PROTEIN CIAO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="31351" checksum="8217A3430887AD3B" modified="2012-10-03" version="1">MRYRVTSKRLNEKILAVHSNGAVYTGGTSRTLTNQDTGEVMCKTQKSIRSIASHGKYMCCGSYDCTAVLFCDGNVVDVIEGPDTEVKCVAFSEDGKYLAMATRGKSVWVVRIDGEIEIDEIIEDHLHDVKGCIFHGGFLFTYGYDNTVKVYDRFDYDDSWELVQSIDEGSTVWCVIFHEGRMICTTEEGMVNSYILRNGWELEISKRLSMFPIYSVCSVGRHMAYTLNRSSIGIIDTDLSIVTSIENVHEDFINGIAYDAGKDRIISGGDDGILNIIEPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7APP7</accession>
    <name>I7APP7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_100320</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265346.1">
        <property type="nucleotide sequence ID" value="XM_009267071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APP7"/>
    <dbReference type="GeneID" id="20564462"/>
    <dbReference type="KEGG" id="ero:EROM_100320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100320"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_1_1"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF24">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="32"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="213"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="397" mass="45072" checksum="1EB64FA8464DE6CF" modified="2012-10-03" version="1">MKFDELRVDESLIRICEGNGITKPTEIQEQVIPVILGGNDIIAVSQTGSGKTLAFVLPIVSCLLLKDRSFYCLIIAPTRELSSQIAECFNMFEETGLRVCLLVGGTSFNVQANQLSKHPHVVVGTPGRIAEHVLKTKSFRIERVRKLVLDEADRFFEQDFVDDLETIIRSLKEKRQTLLFTATMSDEISKLSNSILRNPKIIRVVEKYETVPTLKEYYIFIAMKWKNASLVELLEMNPGISVIVFVSMCITARVMSLALSKLGFHSEALHGELIQEKREETMRMFKESKFNVLVCTDVGSRGLDISHVDLVINFDVPKNGKDYIHRVGRTARAGRSGTAITLVTQYDVEQFQRMEFALKKSLEEFRPMKKDFGLTCKKIGEAIESAQEILKEERNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7APR9</accession>
    <name>I7APR9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MutS-like DNA mismatch repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_100580</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265371.1">
        <property type="nucleotide sequence ID" value="XM_009267096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APR9"/>
    <dbReference type="GeneID" id="20564488"/>
    <dbReference type="KEGG" id="ero:EROM_100580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100580"/>
    <dbReference type="HOGENOM" id="CLU_002472_3_1_1"/>
    <dbReference type="OrthoDB" id="10252754at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032301">
        <property type="term" value="C:MutSalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF148">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="DNA repair protein MutS, domain I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="DNA repair protein MutS, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="7">
        <location>
            <begin position="807"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="15"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="914" mass="106332" checksum="A95C7650D68348C6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7APT3</accession>
    <name>I7APT3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265386.1">
        <property type="nucleotide sequence ID" value="XM_009267111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APT3"/>
    <dbReference type="GeneID" id="20564503"/>
    <dbReference type="KEGG" id="ero:EROM_100730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100730"/>
    <dbReference type="HOGENOM" id="CLU_733843_0_0_1"/>
    <dbReference type="OrthoDB" id="2190797at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="360" mass="41874" checksum="6C47CDD66B41AD78" modified="2012-10-03" version="1">MRRIADLKVRSKRCLTQRAISCPVLRISWDLVYWMARYDKRLRVLCKHMSPREISVLAPWKKSYERRGFYESRASFDIDLHPFVNLRALRVVDSSIFDMFSINNTCIKELKLLKVLGVRMKSPVNTVLEEITLKDTRIPYEDMVSILGIESLISVSLMNVDVSGCHRWREKLLEKIREMPKLKRIVLVNMKVDIQQFTDLCIERGLGWFKIAEEDVCLDVRLCSFASSIRCVGALRCLIGLNYEMVEMMHVEGRDLKYMKETLPNLKVLYVSQAEIDNKTFKTVYLRYPNLIGLGFSECIFNGTSFYETIIHFKGVLRFLDLTSSRLPHDYISFLKKTLCSCSVKLRSGDLIMVDNTSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7APW8</accession>
    <name>I7APW8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_101090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265421.1">
        <property type="nucleotide sequence ID" value="XM_009267146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APW8"/>
    <dbReference type="GeneID" id="20564539"/>
    <dbReference type="KEGG" id="ero:EROM_101090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101090"/>
    <dbReference type="HOGENOM" id="CLU_034027_3_3_1"/>
    <dbReference type="OrthoDB" id="2275560at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000001">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="206" mass="24007" checksum="728D4646AC804B15" modified="2012-10-03" version="1">MENLLEDDSENLLRMSPDEYWVGDFMHRKENHAIVRVPDEFLEDRFNIIGLDRYIKNLEETYNSILDKGPRRNLKEESSLYYLIHQRYIFTKSGLEAILDKVMSREYGVCPRVGCKSSVGMVPVGLSDGPSKSSTKIYCHNCVNVYEPKGNLQLLDGCAWGRSFPHFLILAHSYHFPSRMYEEYVPRIYGFRICSHDDNDSFSEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7APX8</accession>
    <name>I7APX8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263864.1">
        <property type="nucleotide sequence ID" value="XM_009265589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APX8"/>
    <dbReference type="GeneID" id="20520649"/>
    <dbReference type="KEGG" id="ero:EROM_010220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010220"/>
    <dbReference type="HOGENOM" id="CLU_1896188_0_0_1"/>
    <dbReference type="OrthoDB" id="2194316at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707408" evidence="2">
        <location>
            <begin position="18"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="134" mass="15101" checksum="91868D55328499B3" modified="2012-10-03" version="1">MKLYGTVILCLPFLAMCASEVSQHVADTMSNRAQAPYKRVMEHGKTCVGKVCNLFEKGKNMLYTRIKNAMSSIRSKSEKAKEVAGSENMQGSEEEAFDLSPEDIEKLIEEVKKKLAGYMDVAEEKNEEEEKKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7APZ4</accession>
    <name>I7APZ4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_101340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265446.1">
        <property type="nucleotide sequence ID" value="XM_009267171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APZ4"/>
    <dbReference type="GeneID" id="20564564"/>
    <dbReference type="KEGG" id="ero:EROM_101340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101340"/>
    <dbReference type="HOGENOM" id="CLU_035750_5_2_1"/>
    <dbReference type="OrthoDB" id="7854943at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 8 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="202" mass="22185" checksum="94DD6AAAAF9F1215" modified="2012-10-03" version="1">MFNEREKTGTTIIAIKYDGGVLIGADSRTSMGTYIPSRVTDKLTQITNKIFVCRSGSSADTQMISEYLKMYLNMYSRLESSVPSVHRAATLASKIIYENPTLLAGLIVAGYDDKPRVFNISLGGSLMECDWAIGGSGSAFIYGYCDMNWRSGMSLEEGIKFIKDAVSSAIRRDNASGGCIRMSAISRDGVQRYFYPGDKVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ19</accession>
    <name>I7AQ19_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RRM motif-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_010630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263904.1">
        <property type="nucleotide sequence ID" value="XM_009265629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ19"/>
    <dbReference type="GeneID" id="20520690"/>
    <dbReference type="KEGG" id="ero:EROM_010630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010630"/>
    <dbReference type="HOGENOM" id="CLU_115018_0_0_1"/>
    <dbReference type="OrthoDB" id="639027at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="84"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="163" mass="18568" checksum="24960BD7E5889629" modified="2012-10-03" version="1">MEIVKFNPDTKGIERRRGIKTFGDTSRIRVVKSTAAPKFNLYTTEIGIEYEEEEKQHTPYRPSLEEPTTSNAYVPPTIKRPETCSVKLSNLPLDMTRDRLYSIIKSHTSVFFMSPNLVMNRETGVFRGFAFVTLESKDDAIRFIKDLRGVAIDSLGLSAEMAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQQ0</accession>
    <name>I7AQQ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chromobox protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_030080</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264124.1">
        <property type="nucleotide sequence ID" value="XM_009265849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQQ0"/>
    <dbReference type="GeneID" id="20520915"/>
    <dbReference type="KEGG" id="ero:EROM_030080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030080"/>
    <dbReference type="HOGENOM" id="CLU_045874_2_0_1"/>
    <dbReference type="OrthoDB" id="433924at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00024">
        <property type="entry name" value="CD_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017984">
        <property type="entry name" value="Chromo_dom_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008251">
        <property type="entry name" value="Chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023779">
        <property type="entry name" value="Chromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051219">
        <property type="entry name" value="Heterochromatin_chromo-domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22812">
        <property type="entry name" value="CHROMOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01393">
        <property type="entry name" value="Chromo_shadow"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00504">
        <property type="entry name" value="CHROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00300">
        <property type="entry name" value="ChSh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00598">
        <property type="entry name" value="CHROMO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromo" evidence="4">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="18320" checksum="9BFB533656EF74BE" modified="2012-10-03" version="1">MKDGDIYTVDKIVGHRRRRGTKQYLVKWEGYSDSENTWEDEKNIFCKKLIKEYEESQRKLKKGNRQVNKKKAVPRKSKKDLPSSSRCVTNDWDELVDRIVSVEKNGPILVVWLLFKDGKKDRFPASEIHIRCPLHLLEYYESNLVFCEDGVNDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQS8</accession>
    <name>I7AQS8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ARF GTP-binding ADP ribosylation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264154.1">
        <property type="nucleotide sequence ID" value="XM_009265879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQS8"/>
    <dbReference type="GeneID" id="20520947"/>
    <dbReference type="KEGG" id="ero:EROM_030360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030360"/>
    <dbReference type="HOGENOM" id="CLU_040729_12_4_1"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00878">
        <property type="entry name" value="Arf_Arl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="23"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="184" mass="21032" checksum="DDBA2CD0754CA770" modified="2012-10-03" version="1">MYFNKIVRRIKKERAQLRILVLGLDNAGKTTILHKILSKPIEDVHPTFGYQIYDVSYKATSLIILDIGGQSMFIEYWSSYYEDVDGVVFVFDSSDSRSFVEHIGHIRSILVSTPMLILANKSDLNPMFNPNQFGKDFCELLKKKDVKLIKCCGIKGDGLEAGFDWMLGRAMERLVLDEGEKKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR00</accession>
    <name>I7AR00_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcriptional activator HAP2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_031400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYA/HAP2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264259.1">
        <property type="nucleotide sequence ID" value="XM_009265984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR00"/>
    <dbReference type="GeneID" id="20521054"/>
    <dbReference type="KEGG" id="ero:EROM_031400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031400"/>
    <dbReference type="HOGENOM" id="CLU_2015248_0_0_1"/>
    <dbReference type="OrthoDB" id="1097733at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001289">
        <property type="entry name" value="NFYA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12632">
        <property type="entry name" value="TRANSCRIPTION FACTOR NF-Y ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02045">
        <property type="entry name" value="CBFB_NFYA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00521">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51152">
        <property type="entry name" value="NFYA_HAP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="137" mass="16350" checksum="EA30C8E77654DE3E" modified="2012-10-03" version="1">MKQVRKEIYKSFSKVVFMQHRDMMDQGTNARYGDIEFDQEQLRDIGYPGKGGYSAYDYSFPFNDQAFQPLYVNAKQLNWIKKRKARRDMLDTLMVTSKRNYLHESRHKHAMKRLRAPSGRFLTKEETEELNRKGNSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARC3</accession>
    <name>I7ARC3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha-tubulin suppressor-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_041530</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264414.1">
        <property type="nucleotide sequence ID" value="XM_009266139.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARC3"/>
    <dbReference type="GeneID" id="20521216"/>
    <dbReference type="KEGG" id="ero:EROM_041530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041530"/>
    <dbReference type="HOGENOM" id="CLU_005210_6_2_1"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF1">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="3"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="54"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="102"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="154"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="214"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="311"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="359" mass="38847" checksum="D4A73D14FF1CAA96" modified="2012-10-03" version="1">MGVFVFGSNAISQLGLGEDEGSTHVPTRLSFFDGLSPAKVRCGSLHTLILDSDGTLYSWGCNDEGALGRDGDESTPKKVCLREKVVDMDCGASISAALTSSGHVYVWGTFRSTNGAFGLTPRESISFVPIKVPLKNIRALSAGQSFIAALDLKDAIFTFGSNEFGELGRRTSERNKLRALIPDAVTTPRKRLQNYRFKSIGCGLNHLIALNADGEAYCWGSNLYGQLGHDKAESTFNKYKVPLDGIVQAVGGENHSLFITRDGSLYGCGRNKEGQLGIQSTESIRSPVRLGLDGVEAVRSYNSFNICKIGNELYSWGTGFNGALGHEEETLFSPKKIPFDFPSVIDFDVGNDFSVIVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARH2</accession>
    <name>I7ARH2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP1/Obg developmentally regulated GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_050390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264469.1">
        <property type="nucleotide sequence ID" value="XM_009266194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARH2"/>
    <dbReference type="GeneID" id="20521271"/>
    <dbReference type="KEGG" id="ero:EROM_050390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050390"/>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_1"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17230">
        <property type="entry name" value="TGS_DRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001436">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="63"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="284"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="362" mass="39559" checksum="7875CD2A051F9CB1" modified="2012-10-03" version="1">MNVMEKIKEIENEMARTQKNKATSNHLGILKAKLAKLRRELMSVSKSSSSGEGGFDVAKTGIARVGFVGFPSVGKSTLMSKLTGTFSAVAAYEFTTLTTVPGVLSYNGAKIQILDLPGIIEGAKDGKGRGKQVLAVARTCSLIIVCLDVLKPVSHKAVIERELEGVGIRLNKQPPKIKVTKKDKGGVTLIGDSLDLATVKAVLGEFRISNAEVITNGPCTIDDLIDVIEGNRKYVPCIYVLNKIDSISVEELDLLHKIPHSVPVCAHLGWNFDSLLSKMWDYLDLVRIYPKPKGQPIDYEEPVILRSSRRSVSDFCNAIHRGILAKFKHALVWGTSVKHNPQKVGKEHILNDSDVVQIVKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASE4</accession>
    <name>I7ASE4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NadF-like NAD kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_071010</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264849.1">
        <property type="nucleotide sequence ID" value="XM_009266574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASE4"/>
    <dbReference type="GeneID" id="20521661"/>
    <dbReference type="KEGG" id="ero:EROM_071010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071010"/>
    <dbReference type="HOGENOM" id="CLU_008831_5_0_1"/>
    <dbReference type="OrthoDB" id="24581at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD+ metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275:SF43">
        <property type="entry name" value="BIFUNCTIONAL NADP PHOSPHATASE_NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20275">
        <property type="entry name" value="NAD KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="26340" checksum="13984E530804B786" modified="2012-10-03" version="1">MGRFLIVTKNGNDAELVEKIRHSLDCHTLDQGIEYDGIIVLGGDGTVLRTIASYRNPPAVYAVNRGRVGFLCPIPHTAIDELIGRLRNGKRIELVEVKRLCLSKKHYFLNDAVIRPSSIGLGTFKIFIDDISLVLRGDAVIVATKTGSSAYNASLNGPLLLNEGMVVNVVAPNNCRFKPIVCGIGTRVRVEVDRDAQIILDGVLCEEESFDVCYDGSSVRFGHLGHYDQSKRIEELFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASU5</accession>
    <name>I7ASU5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265014.1">
        <property type="nucleotide sequence ID" value="XM_009266739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASU5"/>
    <dbReference type="GeneID" id="20521836"/>
    <dbReference type="KEGG" id="ero:EROM_081020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081020"/>
    <dbReference type="HOGENOM" id="CLU_404908_0_0_1"/>
    <dbReference type="OrthoDB" id="2192004at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="681" mass="80825" checksum="19C97BD78F5248A1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASW3</accession>
    <name>I7ASW3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNase P subunit p30-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_081210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265034.1">
        <property type="nucleotide sequence ID" value="XM_009266759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASW3"/>
    <dbReference type="GeneID" id="20521856"/>
    <dbReference type="KEGG" id="ero:EROM_081210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081210"/>
    <dbReference type="HOGENOM" id="CLU_100281_0_0_1"/>
    <dbReference type="OrthoDB" id="17948at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="24787" checksum="2B4B04225B3168A4" modified="2012-10-03" version="1">MYFDMNVSSRCLGPELMDVLRQLGHDGICVNTVVELDIVKKYKKPSFDGWKDRRIYERVEIKYKDCEDWDDVRKDIDPDVVAVRLEGMKDFKKVLEMRPDVITFDYTKSFCLGRDEFIEATKRNIFFEIPLVCGMYSQRSKAMWMRNARRLLNVTGGVNVVVGSGATCLTEMRGGQDIIKILWGLGMSEDNGRKVLLNSARLVRSCDVRRYVMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATC9</accession>
    <name>I7ATC9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S28</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090650</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265179.1">
        <property type="nucleotide sequence ID" value="XM_009266904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATC9"/>
    <dbReference type="GeneID" id="20564289"/>
    <dbReference type="KEGG" id="ero:EROM_090650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090650"/>
    <dbReference type="HOGENOM" id="CLU_178987_2_2_1"/>
    <dbReference type="OrthoDB" id="10258930at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="65" mass="7404" checksum="47B268B782C7CD98" modified="2012-10-03" version="1">MEEQQEFFGKVTHVLGRTGGSGLLTQVKMELMHNKRTIQRAVKGPVAVGDIIEVLECEREHRKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATH6</accession>
    <name>I7ATH6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091410</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265254.1">
        <property type="nucleotide sequence ID" value="XM_009266979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATH6"/>
    <dbReference type="GeneID" id="20564365"/>
    <dbReference type="KEGG" id="ero:EROM_091410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091410"/>
    <dbReference type="HOGENOM" id="CLU_033361_2_1_1"/>
    <dbReference type="OrthoDB" id="1611972at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="383" mass="42985" checksum="83BFA60303873079" modified="2012-10-03" version="1">MSCRKFSAPRHGSLQFCPRKRSKTIRPSAGAFPADDSSQPVHLTGFMGYKAGMTHVIRTKIQAAKNKQLSREVMDAVTVLEAPPMVVYGVVGYERTVTGLRRLPIVLAPYVSDGVLRRMFGNKHVERENSKRFCASEETESRISEIKKKAECVRVLVQTQPGLIKKLGLKKAHIAEIQVNGGSISEKVEWCLDMLEKEIHIGEVFKENENIDVIGVTKGKGFQGTVKRFGVRKQPRKSRKGIRKVACIGAWHPSRVMYSVARAGQMGFHRRTEKNKRVYMVGNGNSSIRTEFDLTEKFISPMGGFPHYGEVKNDFIMVKGAVVGPRKRVVTLRKSLDQQRPSEELVIKFVDTSSKIGHGRFQTSDEKRAFYGAKKAEVAPEIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATQ4</accession>
    <name>I7ATQ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidyl inositol-3-kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_100460</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265359.1">
        <property type="nucleotide sequence ID" value="XM_009267084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATQ4"/>
    <dbReference type="GeneID" id="20564476"/>
    <dbReference type="KEGG" id="ero:EROM_100460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100460"/>
    <dbReference type="HOGENOM" id="CLU_618240_0_0_1"/>
    <dbReference type="OrthoDB" id="67688at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052742">
        <property type="term" value="F:phosphatidylinositol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="3">
        <location>
            <begin position="176"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="445" mass="51769" checksum="F427A47BEC93B9F4" modified="2012-10-03" version="1">MEEWNSSTFRCMVEEVHISSLGESVSKLLEDSDKDIEKAVAKYLCLQEVDGLYMYFPSFSEVDIEDKLSISHQVSRFWKVRFELREGCARSSHKYEDVVNGFLALDAKYGMLGMLEGQMALIEEIRRIYAITSHKSSKKKRIREYKLYGALRFYSRLGGMVYTSLFNPGTTVKEIVFKEIEIFPSSTFPILVPLDTDRGTSRVMYKKGDDLTKDLFVLETIKYMSRLIGVDLVAYRVIPLSREEGIVEVVDGVDFTKIRKRSDLVRYIEEEKCSDYGRSLEKKKMFVDTLCGYSVACYVMGVGDRNPGNMMVAKDGRFFHIDFSYVFGNEPKPISHRITIVSPIRDYLVNDELAYQDFLSKSGEMFLQIRRSWRKIFVLWCILVRNEIFRLDLDKIISFARDRLKLEITEKEALEHFEKEIKSAVGSFRTSVVHLVNRIGILWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATR0</accession>
    <name>I7ATR0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dynein heavy chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265364.1">
        <property type="nucleotide sequence ID" value="XM_009267089.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20564481"/>
    <dbReference type="KEGG" id="ero:EROM_100510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100510"/>
    <dbReference type="HOGENOM" id="CLU_227616_0_0_1"/>
    <dbReference type="OrthoDB" id="286107at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097729">
        <property type="term" value="C:9+2 motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060294">
        <property type="term" value="P:cilium movement involved in cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.100">
        <property type="entry name" value="Dynein heavy chain, N-terminal domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042222">
        <property type="entry name" value="Dynein_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024743">
        <property type="entry name" value="Dynein_HC_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013602">
        <property type="entry name" value="Dynein_heavy_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10676:SF396">
        <property type="entry name" value="DYNEIN AXONEMAL HEAVY CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10676">
        <property type="entry name" value="DYNEIN HEAVY CHAIN FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08393">
        <property type="entry name" value="DHC_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12777">
        <property type="entry name" value="MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="1589"/>
            <end position="1706"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="1806"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2646"/>
            <end position="2673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="3135" mass="365290" checksum="8B6C2BB02A99F0CC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATX0</accession>
    <name>I7ATX0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_101170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265429.1">
        <property type="nucleotide sequence ID" value="XM_009267154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATX0"/>
    <dbReference type="GeneID" id="20564547"/>
    <dbReference type="KEGG" id="ero:EROM_101170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101170"/>
    <dbReference type="HOGENOM" id="CLU_004962_0_5_1"/>
    <dbReference type="OrthoDB" id="2192794at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="114"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="302" mass="35010" checksum="EAFF35C56CA2E851" modified="2012-10-03" version="1">MTEGLGEEKYFAMLGNIKNSILPTREETLSLIKEVQKVFESEPNILHIDTSVNVVGDVHGHFYDVLNMLSLMEDPSEKTYLFLGDYVDRGYNSVDLILLLFLYKRLYKGNLYLLRGNHETRMLSSVYGFKEECVRKYDLVVYWRICEAFQYLPVAAIVLRKYFCVHGGLVPGMSLDFIMKHDRVGEITEINDILWSDPDETPGFRKSQRGAGYLFGEDVLKNFLNEHGLSHFVRSHQLVNDGYKAYFGEKLYTVWGAPNYCYSSGNIACVMAIDESGHDFRLFDRCEEQYKEIPMRYDFFNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATY0</accession>
    <name>I7ATY0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_101270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265439.1">
        <property type="nucleotide sequence ID" value="XM_009267164.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20564557"/>
    <dbReference type="KEGG" id="ero:EROM_101270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101270"/>
    <dbReference type="HOGENOM" id="CLU_000862_0_0_1"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000079">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000001">
        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000009">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase HECTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050409">
        <property type="entry name" value="E3_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF440">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE NEDD-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="2067"/>
            <end position="2403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1431"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1581"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1431"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1592"/>
            <end position="1601"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="2370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="2403" mass="278055" checksum="E9BFADA3AE67BD11" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGS0</accession>
    <name>I6ZGS0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263880.1">
        <property type="nucleotide sequence ID" value="XM_009265605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGS0"/>
    <dbReference type="GeneID" id="20520665"/>
    <dbReference type="KEGG" id="ero:EROM_010380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010380"/>
    <dbReference type="HOGENOM" id="CLU_1570639_0_0_1"/>
    <dbReference type="OrthoDB" id="2186865at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="170" mass="19251" checksum="5A65E3836D6B014E" modified="2012-10-03" version="1">MNWVLSLCCGTTRKKYVITLLGLQEDQRHVLKHSISSYNMDKPLRPFSEDICIANGMELILQSLDDNADLYEIRGIHMGLAAGVIFAVDADNKSSVEKCIALINEDTTKTKDIIVVLCRSNIYGNESVQELKRTCDEIGHGKIEFILYEESRSFLGVHKGINWICNRLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGV8</accession>
    <name>I6ZGV8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263920.1">
        <property type="nucleotide sequence ID" value="XM_009265645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGV8"/>
    <dbReference type="GeneID" id="20520706"/>
    <dbReference type="KEGG" id="ero:EROM_010790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010790"/>
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    <dbReference type="OrthoDB" id="2190965at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1231" mass="139656" checksum="9B17FF5EBCACF7C7" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH16</accession>
    <name>I6ZH16_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263980.1">
        <property type="nucleotide sequence ID" value="XM_009265705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH16"/>
    <dbReference type="GeneID" id="20520766"/>
    <dbReference type="KEGG" id="ero:EROM_020080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020080"/>
    <dbReference type="HOGENOM" id="CLU_1326361_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003706999" evidence="2">
        <location>
            <begin position="18"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="207" mass="22979" checksum="032977F1E09DEA84" modified="2012-10-03" version="1">MKSRPLAFVCFVITINAGFFDLFSGSSNSQKSSSNEEEENKNVELSQEQLVNKIMSEVEDIDKSVLQLNDEVEKEKKEKLDSESQKLQTALSSWKQLQEAAKNAENEKKKQKKKTNSMNALKKEIPRHTNAIKKKVGKVVNGTNNEQEGDESDGGDESDGDGKSGRGGKGKVLKKLLGIGKSSKKRKRNPSSSKGKRKKFKSSKNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHC1</accession>
    <name>I6ZHC1_ENCRO</name>
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        <submittedName>
            <fullName evidence="2">Rad4-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_021230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264095.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHC1"/>
    <dbReference type="GeneID" id="20520885"/>
    <dbReference type="KEGG" id="ero:EROM_021230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021230"/>
    <dbReference type="HOGENOM" id="CLU_530068_0_0_1"/>
    <dbReference type="OrthoDB" id="342264at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000781">
        <property type="term" value="P:positive regulation of double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990166">
        <property type="term" value="P:protein localization to site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00027">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17738">
        <property type="entry name" value="BRCT_TopBP1_rpt7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042479">
        <property type="entry name" value="Slf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46677">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46677:SF1">
        <property type="entry name" value="SMC5-SMC6 COMPLEX LOCALIZATION FACTOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16770">
        <property type="entry name" value="RTT107_BRCT_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="174"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="284"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="552" mass="63978" checksum="BAC39DAE709B1F7C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ51</accession>
    <name>I6ZJ51_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Signal peptidase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264745.1">
        <property type="nucleotide sequence ID" value="XM_009266470.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ51"/>
    <dbReference type="GeneID" id="20521555"/>
    <dbReference type="KEGG" id="ero:EROM_061580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061580"/>
    <dbReference type="HOGENOM" id="CLU_080493_1_0_1"/>
    <dbReference type="OrthoDB" id="243127at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041131">
        <property type="entry name" value="RicT_YaaT_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="1">
        <location>
            <begin position="132"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="230" mass="27349" checksum="6EF48A6B734C3BAB" modified="2012-10-03" version="1">MFNHPEWISYHPNSIWNETNEKWDEGDMGWRTVGVTPNRFIDIPGDIETKEKGILPISFLDMFPRRWYSVVEFPSGRLEIGYYSNIIDLDIYVILEADRGEDCGKIIGNMSEHEFESSVLSLRDMKAEFEPKKILRKASADDLSKLKQRKKIEIESLRQCKELVSARNLNMEILSCEYQWDMKKITFYFKSDKRIDFRDLLKELFKIFKVRIWMCAERRTNNDLIKKILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJA5</accession>
    <name>I6ZJA5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264815.1">
        <property type="nucleotide sequence ID" value="XM_009266540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJA5"/>
    <dbReference type="GeneID" id="20521626"/>
    <dbReference type="KEGG" id="ero:EROM_070670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070670"/>
    <dbReference type="HOGENOM" id="CLU_1570638_0_0_1"/>
    <dbReference type="OrthoDB" id="2191587at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19304" checksum="7E0415703AFC3122" modified="2012-10-03" version="1">MIECSVFGYLKEIDCICNYLRLCKRTDKIATESIFSNGLNVLLVSEESDGVYLISRGAPDRNKNRMSLCSKVQRSRVRTLGTLKVFLFSLGFELVRQGKVELITFEKEPGHIEISKHVSEESSKHKETDDYYLVKVFVISENAHDGERMLSRVIEELEGQVQLVKPSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJG9</accession>
    <name>I6ZJG9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dopey-like leucine zipper transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_071370</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DOP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264885.1">
        <property type="nucleotide sequence ID" value="XM_009266610.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521697"/>
    <dbReference type="KEGG" id="ero:EROM_071370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071370"/>
    <dbReference type="HOGENOM" id="CLU_247152_0_0_1"/>
    <dbReference type="OrthoDB" id="297643at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006895">
        <property type="term" value="P:Golgi to endosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040314">
        <property type="entry name" value="DOP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056457">
        <property type="entry name" value="DOP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007249">
        <property type="entry name" value="DOP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042">
        <property type="entry name" value="DOPEY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14042:SF24">
        <property type="entry name" value="PROTEIN DOPEY-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24598">
        <property type="entry name" value="DOP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04118">
        <property type="entry name" value="Dopey_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DOP1 N-terminal" evidence="5">
        <location>
            <begin position="3"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="DOP1-like C-terminal" evidence="6">
        <location>
            <begin position="1245"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="439"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00046326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF24598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1562" mass="180191" checksum="35A57CBE873AE1F1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKC8</accession>
    <name>I6ZKC8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091120</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265225.1">
        <property type="nucleotide sequence ID" value="XM_009266950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKC8"/>
    <dbReference type="GeneID" id="20564336"/>
    <dbReference type="KEGG" id="ero:EROM_091120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091120"/>
    <dbReference type="HOGENOM" id="CLU_035750_3_0_1"/>
    <dbReference type="OrthoDB" id="429533at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 8 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="227" mass="24868" checksum="D49F866E4C27EB88" modified="2012-10-03" version="1">MIVKTGTTIVGMKYKTGVILAADTRSTQRPVVSDRNCVKIHRITDKIMCCGAGTAADAERVTRMASKELRLFENKYFRSPLVSHFKKVCTHHLHRYGGGIGAALIVGGIDDEGCHLYDIHPHGSSSPVLFTSLGSGSLAAIAMLEGKYRLMDKGEAIELACDAVKAGVLNDLYSGSNIDVCVIDYSGVEFLRNYKRIGVSESTDTLVYPLDSIRIKKEEVFDIVEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKF8</accession>
    <name>I6ZKF8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265270.1">
        <property type="nucleotide sequence ID" value="XM_009266995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKF8"/>
    <dbReference type="GeneID" id="20564381"/>
    <dbReference type="KEGG" id="ero:EROM_091570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091570"/>
    <dbReference type="HOGENOM" id="CLU_157452_0_0_1"/>
    <dbReference type="OrthoDB" id="2192698at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031533">
        <property type="entry name" value="DUF5093"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17011">
        <property type="entry name" value="DUF5093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="130" mass="15283" checksum="2034B8C509EB189E" modified="2012-10-03" version="1">MSILEIEFPFRIGETHPRLKMEVEMKRKEDLVSFCMEYDMDLVIDNAELKSKEEIRGQFVYAYKFGDLDSAIEFMENSQAKALATRGLLDVEKVEKEMDLFMEKYETGEKRSKKKKRAIVVGEDGFMKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLB2</accession>
    <name>I6ZLB2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_111370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265615.1">
        <property type="nucleotide sequence ID" value="XM_009267340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLB2"/>
    <dbReference type="GeneID" id="20564736"/>
    <dbReference type="KEGG" id="ero:EROM_111370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111370"/>
    <dbReference type="HOGENOM" id="CLU_2062180_0_0_1"/>
    <dbReference type="OrthoDB" id="2191306at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="13221" checksum="3236379A8BCD34DB" modified="2012-10-03" version="1">MDREVLRHRVLVTIGKSSLFRFIENVLEEGKIPTMASSITEYLLNNKYNKEKALDHISNYLEEELENSGIDVDDGADGVALAVLFVYEEFLENKSKFFSKIQEKSSQVSPLSDSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZRY9</accession>
    <name>I6ZRY9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263848.1">
        <property type="nucleotide sequence ID" value="XM_009265573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZRY9"/>
    <dbReference type="GeneID" id="20520632"/>
    <dbReference type="KEGG" id="ero:EROM_010060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010060"/>
    <dbReference type="HOGENOM" id="CLU_625597_0_0_1"/>
    <dbReference type="OrthoDB" id="2191239at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="438" mass="50021" checksum="0FEA0D3F6444348E" modified="2012-10-03" version="1">MLPNEIDPYEFKFFVKDILPLSETYTLFKSSKLLKAKETVKAISKTNQGLIVLIHKPKETTIYTPESKVSYFTKRRGRMAPKNAVIRFLKAKMVDFVVLRFKAFYCCLSRWDKDVCSFCIVACLGKGATERALLNPSEILGDVKETPPLIGKGMFKFKDLDSFKRLRIGHKDQSQEANCSSVSESPSSASDVKVNPIDFVQEDGLEYINKSASGNTDGLPDRKTEDKNCLVEIMSEGTKEATENIYSKPYKEVKNDEEGLIDYIISKITKIVCEDVNTGCLQEDGEKDKLWKTLEEDEESDGSSKGCGYDFEGNNFCDDKQNKGYGSYSHEDQDESFKYDNTIRFGIIDDGKLRCIQKSVGLLSMAKFDRLEAFIIGIIDRLKKTNINYVEVCSEIDFFIVKKQDTIHLYVRDLIHRKERKFMENFNEIFEKLGARTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS46</accession>
    <name>I6ZS46_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldo-keto reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010770</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263918.1">
        <property type="nucleotide sequence ID" value="XM_009265643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS46"/>
    <dbReference type="GeneID" id="20520704"/>
    <dbReference type="KEGG" id="ero:EROM_010770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010770"/>
    <dbReference type="HOGENOM" id="CLU_023205_0_0_1"/>
    <dbReference type="OrthoDB" id="416253at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000002">
        <property type="entry name" value="2,5-diketo-D-gluconic acid reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11732">
        <property type="entry name" value="ALDO/KETO REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="17"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="301" mass="34136" checksum="33927E2B97CB8781" modified="2012-10-03" version="1">MVCRTQKLNNGHEIPTVGLGTWGLEDESSLEASIRNAISLGYRHIDTAFIYGNEKMIGNILKKLFNEGVVQRKDLFITSKLWNTFHHCPEDALRQTLDDLQTSYVDLYLIHWPVTFEPAPNGSVESCGKKYNIGEFDAVSVWKKMEELVDLGLAKSIGISNFGKENTEKVLKACRIRPAVSQFELHPYLNQKELVEFMQSEKIQVISYSSLGSGHDSSQKVREDKTIKAIAKKYECTPSQIILSYIVSRNICVIPRSKSKEHLKENIDLKELAKEDISIIDGLDIGHRYVDPVSFGPNRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSE2</accession>
    <name>I6ZSE2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264023.1">
        <property type="nucleotide sequence ID" value="XM_009265748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSE2"/>
    <dbReference type="GeneID" id="20520811"/>
    <dbReference type="KEGG" id="ero:EROM_020510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020510"/>
    <dbReference type="HOGENOM" id="CLU_353817_0_0_1"/>
    <dbReference type="OrthoDB" id="2190652at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45617">
        <property type="entry name" value="LEUCINE RICH REPEAT FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="781" mass="89462" checksum="EC68C58DB8AB7ECA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSG2</accession>
    <name>I6ZSG2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S ribosomal protein L9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_020710</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264043.1">
        <property type="nucleotide sequence ID" value="XM_009265768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSG2"/>
    <dbReference type="GeneID" id="20520831"/>
    <dbReference type="KEGG" id="ero:EROM_020710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020710"/>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_1"/>
    <dbReference type="OrthoDB" id="10252633at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="11"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="183" mass="20347" checksum="C09A37CDD906EBBE" modified="2012-10-03" version="1">MKILVTRTVKVPENCTVSQEGKVFTFEGPKGSIVEDCTRHPLTFDIYEGNIRIRLWNGKRKAAALAITVESLLRNAIKAVTVGFAYTLKAAYNHFSINMEVKDGGKILLVKNFLGEKSVKIFKMRGVARVRLDERKDTIVIEGPSLPDVSQSAGTISNECKAKNRDSRVFLDGIFVIERGIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSZ0</accession>
    <name>I6ZSZ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264208.1">
        <property type="nucleotide sequence ID" value="XM_009265933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSZ0"/>
    <dbReference type="GeneID" id="20521001"/>
    <dbReference type="KEGG" id="ero:EROM_030900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030900"/>
    <dbReference type="HOGENOM" id="CLU_821424_0_0_1"/>
    <dbReference type="OrthoDB" id="2192149at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="337" mass="40006" checksum="58E95301559C560D" modified="2012-10-03" version="1">MDWYLKKYPDAKTLAAKLSKLALSDGVDVSEYLDPENLELADHIYTGRLYRSRDVRKVLRRIAGKESSRVERIMYEMFCKRKIVFDNYLLKLSFVRRFGRFKRFFVDGKEVEYKMQMMVHRQLYLGEDVTKEIEKEIMKIPGEKVWVYVGFLGGSLSRDVIMRIMDCVDIEVKPFVGSFLSMEDYYNKMCYAVLKSPFSEKERIVSSMGTLDKAQFYKAMLHVWFSEGAEKRKFIDFVELMRLVKVGRSMKMEIINGYLNGLVYHRMLEEFVKLFEYVRRAGVDEDKVFGRLYSAIRKYKKGGRLELSGFLDRCKEGRRKGLFSPYSWEDIYDVYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT11</accession>
    <name>I6ZT11_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264238.1">
        <property type="nucleotide sequence ID" value="XM_009265963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT11"/>
    <dbReference type="GeneID" id="20521033"/>
    <dbReference type="KEGG" id="ero:EROM_031190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031190"/>
    <dbReference type="HOGENOM" id="CLU_1294391_0_0_1"/>
    <dbReference type="OrthoDB" id="10531175at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="213" mass="24174" checksum="BB8993420D6E0CC9" modified="2012-10-03" version="1">MNDLEKSSLLMFVKAVFHFIVILRIIKEWNHVPDYKMNFETVSKIVLASYVLLLSQKITDDLDSSSITSILISTMMMLAINAIRKKKFESMESVLRPFIDFLSHGLSESTQKKVSSSLENIIPVFLVGHLVVLASGLLLGIEKRFTKYVRHPRDKLLFVYGLILFILGFYISNVGEEIAINIIEKAASNGRIEGAKTFFRGIKPNKSNYLLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT96</accession>
    <name>I6ZT96_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chromosome segregation ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_040890</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264353.1">
        <property type="nucleotide sequence ID" value="XM_009266078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT96"/>
    <dbReference type="GeneID" id="20521153"/>
    <dbReference type="KEGG" id="ero:EROM_040890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040890"/>
    <dbReference type="HOGENOM" id="CLU_001042_0_1_1"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03275">
        <property type="entry name" value="ABC_SMC1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028468">
        <property type="entry name" value="Smc1_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937:SF12">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="11">
        <location>
            <begin position="501"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="228"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="357"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="715"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="800"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1159" mass="135610" checksum="C9B387A74D493B4C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTD7</accession>
    <name>I6ZTD7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Eukaryotic TATA box binding transcription initiation factor TFIID-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_041470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264408.1">
        <property type="nucleotide sequence ID" value="XM_009266133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTD7"/>
    <dbReference type="GeneID" id="20521210"/>
    <dbReference type="KEGG" id="ero:EROM_041470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041470"/>
    <dbReference type="HOGENOM" id="CLU_060161_4_2_1"/>
    <dbReference type="OrthoDB" id="2127950at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04516">
        <property type="entry name" value="TBP_eukaryotes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000001">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000002">
        <property type="entry name" value="TATA-box-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033710">
        <property type="entry name" value="TBP_eukaryotic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22326" checksum="8607A83A1EBA1C7F" modified="2012-10-03" version="1">MDAPDITYEHQDTSVPNRSGIIPTLQNVVATVNLNCKLDLKNIALRARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVITGAKSEKSSKMAAQRYAKIIHKLGFNATFDDFKIQNIVSSCDIKFSIRLEGLAYAHSNYCSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKVRDDIYQAFNNIYPVLIQYKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTJ0</accession>
    <name>I6ZTJ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264478.1">
        <property type="nucleotide sequence ID" value="XM_009266203.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTJ0"/>
    <dbReference type="GeneID" id="20521280"/>
    <dbReference type="KEGG" id="ero:EROM_050480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050480"/>
    <dbReference type="HOGENOM" id="CLU_2061462_0_0_1"/>
    <dbReference type="OrthoDB" id="2195211at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="14143" checksum="2D4D744F6AAD8F89" modified="2012-10-03" version="1">MNSDVLYEVIKYLDFPVRNRRIQEVQLSRNNSRRMMRLNRDLIEKKLSDRPQIIDLRTSNIYKEHGINFHRIHKKLSDVFHRRGSPPFPNVSATLAGTVKIMDFKLRRIILASRIGSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTR1</accession>
    <name>I6ZTR1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="6 16">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_051310</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="6 16"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264558.1">
        <property type="nucleotide sequence ID" value="XM_009266283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTR1"/>
    <dbReference type="GeneID" id="20521362"/>
    <dbReference type="KEGG" id="ero:EROM_051310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051310"/>
    <dbReference type="HOGENOM" id="CLU_023471_1_2_1"/>
    <dbReference type="OrthoDB" id="10260889at2759"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF8">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE, PHOTORECEPTOR-SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="106"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="288"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN83061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="394" mass="45010" checksum="CDF6D23F04B30100" modified="2012-10-03" version="1">MIREKRGCKEDLNGCDSSKTLRRKEESVLNGRLHHEIVQDKIGGITKTNFFRRMVLSIVMISGFFWICMNNKIYSYALVIFLTVSIIKEIIGITRKSDGKPFHLRVSIILGFAVPIYLYLVFPSIMMMYFSEVLRYFFRRLSSICFYSYVAAFMCFVGSLRKGKLRLQLGLFALVNLSTYSMAMVAKCAIFNLNKGRFWFVFPALLVISNDISAYAVGKSIGKRPLYRLSPKKTLEGFIGAFIFTTVVGFILGYLHVNRGFLRDEEPIKLRKSMKFKVFSAVFKIQSIYFHIIPFILVASFVAPFSGFLASALKRAYKKKDFGEAISGHGGIADRMDCQVIIAIFTSTYISSFIYTEDKSVGSIFSFICKNFSPEEIMILIEMLNRRISSIKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU64</accession>
    <name>I6ZU64_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="8">5.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Pentose-5-phosphate 3-epimerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">RPE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">EROM_060990</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-xylulose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="8"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264688.1">
        <property type="nucleotide sequence ID" value="XM_009266413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU64"/>
    <dbReference type="GeneID" id="20521495"/>
    <dbReference type="KEGG" id="ero:EROM_060990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060990"/>
    <dbReference type="HOGENOM" id="CLU_054856_2_2_1"/>
    <dbReference type="OrthoDB" id="1927044at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00411"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000191">
        <property type="entry name" value="ribulose-phosphate 3-epimerase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="16" id="KW-0170">Cobalt</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AFN83191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="210" mass="24006" checksum="8876AB505D306E9E" modified="2012-10-03" version="1">MLKRRVGISILDCNFAKLEEELRELKANGATNIHLDVMDTTFVKNITFGPCIINRILEHDFVFDIHMMVESPLDIVMQIDLERVFLVTIHHEISNKVGVREYLKQRNVLFGIAINPETRMEEIEMEDADFILVMSVRPGFGGQKFQESCLAKIRDIQKHGKIIGIDGGIGMANIEKVVGVDYVVIGSGYFRSEDRRGFLREITEKFLGEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUI2</accession>
    <name>I6ZUI2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264843.1">
        <property type="nucleotide sequence ID" value="XM_009266568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUI2"/>
    <dbReference type="GeneID" id="20521655"/>
    <dbReference type="KEGG" id="ero:EROM_070950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070950"/>
    <dbReference type="HOGENOM" id="CLU_1170954_0_0_1"/>
    <dbReference type="OrthoDB" id="2190002at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="217" mass="25152" checksum="76166329E8BFA076" modified="2012-10-03" version="1">MLLLFFMSVYSFQGRYGSCARPNRRLLHSDYDGVMVLNVLHRNQPLTVFDSNYPERISLPALLSEFSKEMMENQIFDLKYESFSKKIWLELKKERCIEIADVHSALKAVRMKISPSAIYLKRLPNSMKFQLISGGKCLTVGNEINYAGVRGWALSFTDCEDNNDLQTFVMIPSLKGLMKLNEKMRTDKMVHRMYDTFRRIGKIIGVVDITRSGRPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUP4</accession>
    <name>I6ZUP4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Calcineurin subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Calcineurin regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Protein phosphatase 2B regulatory subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_080080</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Composed of a catalytic subunit (A) and a regulatory subunit (B).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264928.1">
        <property type="nucleotide sequence ID" value="XM_009266653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUP4"/>
    <dbReference type="GeneID" id="20521744"/>
    <dbReference type="KEGG" id="ero:EROM_080080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080080"/>
    <dbReference type="HOGENOM" id="CLU_061288_10_3_1"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="26"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="132"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="200" mass="23791" checksum="826D0EA0A5640840" modified="2012-10-03" version="1">MGSLSSTMLCEEEIEELKNITVFDEREIEHLYERFQFLDRESRGYLTYNELNNIPEFQSNPFSHLIMKSIEKMTDYEKMTFPHFLEFLGIFSEKASKKKRVRYLFDIFDLNGDGRLCRNVLIRVNKMMGQDGKMEEVENLLNIYDEGGKGYLDISDFTKFYESDPLIDKNMIIDFSKNLKQRRQVGFMQVLWPSTYKDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUR5</accession>
    <name>I6ZUR5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080340</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264953.1">
        <property type="nucleotide sequence ID" value="XM_009266678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUR5"/>
    <dbReference type="GeneID" id="20521770"/>
    <dbReference type="KEGG" id="ero:EROM_080340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080340"/>
    <dbReference type="HOGENOM" id="CLU_647278_0_0_1"/>
    <dbReference type="OrthoDB" id="2194205at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="426" mass="49611" checksum="546F3113F4A5188E" modified="2012-10-03" version="1">MFKHDFHDPYNMEEYFQISDKTRTLEHQYTDLLTRKLRLETLLVEEDYNNLLVMHSELVSKARDSEDSYKKHINDLKTMIDKLNQDNAKKEDDIRRLTEENKNLMSTVQELEKKSKEESRQGERKIKGGKKGAGDLEEDEIWEVEGLKKMDEIHRIAKSLEGELQSNLSKLQEECIRYRGDTERMASEIEKLKLRNADNIEKINEIKENKADIEEKLKRLEEENARLAKEKICSDELRRSINEKDEIISALKENMKQKSELIEIQRRMIGGGPQESRVILNDDVCNVFDEEILKAEEGNDELELGDECNAMEEFWNDVPIKSKATMRKKPEGVASARRKTNAPKAEKIRRILPKAVTSIEKTKSDINTKVFDRVLDNKEISKTSETSRKLLTPGHLLKPENSSYFADLTFNNSSPVIKKDQLPKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUU6</accession>
    <name>I6ZUU6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-conjugating enzyme E2 H</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">(E3-independent) E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">E2 ubiquitin-conjugating enzyme H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquitin carrier protein H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-protein ligase H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_080830</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. E2 ubiquitin conjugating enzyme that transfers ubiquitin to MAEA, a core component of the CTLH E3 ubiquitin-protein ligase complex. In vitro catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with MAEA and WDR26, components of the CTLH complex that contains GID4, RANBP9 and/or RANBP10, MKLN1, MAEA, RMND5A (or alternatively its paralog RMND5B), GID8, ARMC8, WDR26 and YPEL5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264998.1">
        <property type="nucleotide sequence ID" value="XM_009266723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUU6"/>
    <dbReference type="GeneID" id="20521818"/>
    <dbReference type="KEGG" id="ero:EROM_080830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080830"/>
    <dbReference type="HOGENOM" id="CLU_030988_7_1_1"/>
    <dbReference type="OrthoDB" id="269518at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd23797">
        <property type="entry name" value="UBCc_UBE2H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000078">
        <property type="entry name" value="ubiquitin-conjugating enzyme E2 H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="16">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="14">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00060202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00076312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AFN83501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="19533" checksum="464F0AD11BDBD320" modified="2012-10-03" version="1">MVNLNPRIKNEIQKLYNANYEVRMIEGRHTQMEVLINGPEDSPYSGAKYIINILLPDNYPFKSPSIGFTTRIFHPNVDEASGSICLDVLNQIWSPIYDLLNIVDILLPQLLSYPNAADPLNCEAGSMYLTNREKYNEIVRSYVKKYAIPITKEKIDTETNNNLSDEESLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVD8</accession>
    <name>I6ZVD8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA replication licensing factor MCM7</fullName>
            <ecNumber evidence="6">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MCM7</name>
        <name evidence="8" type="ordered locus">EROM_090740</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="6"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265188.1">
        <property type="nucleotide sequence ID" value="XM_009266913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVD8"/>
    <dbReference type="GeneID" id="20564298"/>
    <dbReference type="KEGG" id="ero:EROM_090740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090740"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="OrthoDB" id="3207464at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008050">
        <property type="entry name" value="MCM7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF26">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01663">
        <property type="entry name" value="MCMPROTEIN7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="7">
        <location>
            <begin position="319"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="694" mass="78258" checksum="32AA94E29EA1A88B" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVH8</accession>
    <name>I6ZVH8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription elongation factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_091250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the ELOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265238.1">
        <property type="nucleotide sequence ID" value="XM_009266963.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVH8"/>
    <dbReference type="GeneID" id="20564349"/>
    <dbReference type="KEGG" id="ero:EROM_091250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091250"/>
    <dbReference type="HOGENOM" id="CLU_105983_2_0_1"/>
    <dbReference type="OrthoDB" id="445983at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.190:FF:000001">
        <property type="entry name" value="Transcription elongation factor 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007808">
        <property type="entry name" value="Elf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038567">
        <property type="entry name" value="T_Elf1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20934:SF22">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05129">
        <property type="entry name" value="Zn_ribbon_Elf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="89" mass="10134" checksum="02143E5439BB53E5" modified="2012-10-03" version="1">MLIPPPSPLMSRKKVKRKSNVPRRQTKLEKRFNCPLCNHENVVQCIVKRTLMKGFANCSVCDANFSCDANKLTTGIDVYSAWVDECCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVI8</accession>
    <name>I6ZVI8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sec61beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265248.1">
        <property type="nucleotide sequence ID" value="XM_009266973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVI8"/>
    <dbReference type="GeneID" id="20564359"/>
    <dbReference type="KEGG" id="ero:EROM_091350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091350"/>
    <dbReference type="HOGENOM" id="CLU_187038_0_0_1"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="85" mass="9468" checksum="6EBF1B1E6B140223" modified="2012-10-03" version="1">MVKQISGKIQQYKKRQSKLNELLYNQPSRLLISPMQVLVISLIFIGNVFLLHILAKLVPSSSLPQAAIALMVVLLSIAFSFLSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVR9</accession>
    <name>I6ZVR9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ADF actin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265338.1">
        <property type="nucleotide sequence ID" value="XM_009267063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVR9"/>
    <dbReference type="GeneID" id="20564454"/>
    <dbReference type="KEGG" id="ero:EROM_100240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100240"/>
    <dbReference type="HOGENOM" id="CLU_138659_0_0_1"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00013">
        <property type="entry name" value="ADF_gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="1">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="17894" checksum="AF6AF2CA5545EE6D" modified="2012-10-03" version="1">MSLTIPGLDLIKNEISEIRSGNLRYTIAYLSIKEPISLSVVRSGGRIKNFDYKRKYTIEELHETFEEFLEGINEKDPCFVVYDFIHFDDDGIQKNTLCLISYIPEHLMPLEKVAYSTNVLNLKDLLDISVHIPIKKKSDFIFDDIAEKCAASRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW09</accession>
    <name>I6ZW09_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 6A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_101310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265443.1">
        <property type="nucleotide sequence ID" value="XM_009267168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW09"/>
    <dbReference type="GeneID" id="20564561"/>
    <dbReference type="KEGG" id="ero:EROM_101310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101310"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_1"/>
    <dbReference type="OrthoDB" id="9443236at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000009">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000037">
        <property type="entry name" value="26S protease regulatory subunit 6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="181"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="401" mass="44862" checksum="A87280830E5CB296" modified="2012-10-03" version="1">MSLEELEMMDQSNPEEFLEKIREKIRMLNSETRILQSRVSTIKHDISTKNASIAENMERIRLNKQLPYLVGNVVEVLDDQSAVVNASTRMSSYLPITGLIPNSELRPGDLVALHKDTNIVFEKLPPDYDMKVGGMVLKSDAKPDETYEDIGGLERQIEELNEAIVLSLTYPERFAKLNIKPPKGVLMYGPPGTGKTLMARACASKTNATFLKLAGPQLVQMYIGDGARLVRDAFALAKEKKPTIIFIDEIDAIGAKRSDSDQTGDREVQRTMLELLNQLDGFSSSEEVKIIAATNRVDILDPALLRSGRLDRKIEFPLPNALGRKRILQIHARKMSVRDDVNFDELARSTEGFNGAQCKAVCVEAGMAALRKEKTEISQNDFMDGIQEVLSRKKSKLLYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW14</accession>
    <name>I6ZW14_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homeobox domain-containing transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_101360</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265448.1">
        <property type="nucleotide sequence ID" value="XM_009267173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW14"/>
    <dbReference type="GeneID" id="20564566"/>
    <dbReference type="KEGG" id="ero:EROM_101360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101360"/>
    <dbReference type="HOGENOM" id="CLU_145531_0_0_1"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="85"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="87"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="151" mass="17624" checksum="F46AB2B39A7F5DA3" modified="2012-10-03" version="1">MSKLPNLVSSRINEIRRIYVEANNRIVDEMDSMVDKFVKSIGSVPDISTGVSAYLEERIRYIREFVVDSIGFSISEFRALYNPDGNENARSRRFPKFITEALERSFEIDQYPSETEKSRLAKICKLSTKQINNWFTNKRNRSKSHEGGRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWF4</accession>
    <name>I6ZWF4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Pdcd5 programmed cell death 5-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_111250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265603.1">
        <property type="nucleotide sequence ID" value="XM_009267328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWF4"/>
    <dbReference type="GeneID" id="20564724"/>
    <dbReference type="KEGG" id="ero:EROM_111250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111250"/>
    <dbReference type="HOGENOM" id="CLU_171983_0_0_1"/>
    <dbReference type="OrthoDB" id="2189682at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="84" mass="9915" checksum="18BE0CFFCC8953E1" modified="2012-10-03" version="1">MDQSDELIRKILDEKSLEALGTLKHINKEKGEALQNALVQHFYVTRKLISYNEYIEIARKTEEKNSKPCVNFKRRGNFFELDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWG3</accession>
    <name>I6ZWG3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_111350</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265613.1">
        <property type="nucleotide sequence ID" value="XM_009267338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWG3"/>
    <dbReference type="GeneID" id="20564734"/>
    <dbReference type="KEGG" id="ero:EROM_111350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111350"/>
    <dbReference type="HOGENOM" id="CLU_002794_11_1_1"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04096">
        <property type="entry name" value="eEF2_snRNP_like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EFG_II"/>
    </dbReference>
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        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="17"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="850" mass="94563" checksum="487036BFA1B88DFB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACV3</accession>
    <name>I7ACV3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_010710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263912.1">
        <property type="nucleotide sequence ID" value="XM_009265637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACV3"/>
    <dbReference type="GeneID" id="20520698"/>
    <dbReference type="KEGG" id="ero:EROM_010710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010710"/>
    <dbReference type="HOGENOM" id="CLU_146851_0_0_1"/>
    <dbReference type="OrthoDB" id="10249311at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11843">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="18"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="133" mass="14434" checksum="A4BB904F978E8F1F" modified="2012-10-03" version="1">MSEMQEPMMESEMTLQEALSKVCKVSRTYCKLSRGARETTKKILAGNMRFVMLAKDAEPKIAKLVTLLAKKKDIPIISVESRLELGKIVGVENASSSGKVRSKGCCVAGIQDYCEQTTEAGFVQAALLKGVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACV8</accession>
    <name>I7ACV8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Tat binding protein 1-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_010760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HOP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263917.1">
        <property type="nucleotide sequence ID" value="XM_009265642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACV8"/>
    <dbReference type="GeneID" id="20520703"/>
    <dbReference type="KEGG" id="ero:EROM_010760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010760"/>
    <dbReference type="HOGENOM" id="CLU_063266_2_1_1"/>
    <dbReference type="OrthoDB" id="272266at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120231">
        <property type="term" value="C:DNA recombinase auxiliary factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120230">
        <property type="term" value="F:recombinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007129">
        <property type="term" value="P:homologous chromosome pairing at meiosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010774">
        <property type="term" value="P:meiotic strand invasion involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010776">
        <property type="entry name" value="Hop2_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15938:SF0">
        <property type="entry name" value="HOMOLOGOUS-PAIRING PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15938">
        <property type="entry name" value="TBP-1 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07106">
        <property type="entry name" value="WHD_TBPIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homologous-pairing protein 2 winged helix" evidence="7">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="197" mass="23158" checksum="DD082F96235E4FBB" modified="2012-10-03" version="1">MKEDYEDEEERVFQMMYKCNRPTSKSEIMAYFKGEIGGTAIQEILDYLESDGRLVTKTYGKSKIYLVNQDLFQSEEDVKLNAELQEYGEKRKILEEEISTLDAEIKMLDKMMSINQLEESIKILDKTVKDNQERLESFKNGGREVSKKDMENAKKKYERAQSGLKKIRRIFNEVIERLSEGMDMKKSELYEEIGIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADS2</accession>
    <name>I7ADS2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Regulatory protein RecX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_031080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264227.1">
        <property type="nucleotide sequence ID" value="XM_009265952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADS2"/>
    <dbReference type="GeneID" id="20521021"/>
    <dbReference type="KEGG" id="ero:EROM_031080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031080"/>
    <dbReference type="HOGENOM" id="CLU_1845083_0_0_1"/>
    <dbReference type="OrthoDB" id="2191619at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="139" mass="16582" checksum="45EA04D343585F96" modified="2012-10-03" version="1">MFEAINSVDSKAIKKSEDHERAMLLMEYNKALKDLNVGSFLRYKVKHDVNLGLYKRASGYLISNYTAKKALEEVEQNIERYKLLNYRESLFNMARRNIIERNNFVRARKLLNIAREKGFFCNELYELEELLTTEWYPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE20</accession>
    <name>I7AE20_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S27</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_040980</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264362.1">
        <property type="nucleotide sequence ID" value="XM_009266087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE20"/>
    <dbReference type="GeneID" id="20521162"/>
    <dbReference type="KEGG" id="ero:EROM_040980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040980"/>
    <dbReference type="HOGENOM" id="CLU_130128_3_1_1"/>
    <dbReference type="OrthoDB" id="5567124at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="85" mass="9489" checksum="94004AD63ACF69F2" modified="2012-10-03" version="1">MPSDLAFPTREQIASTYKKDRLYREGNGFFMYVRCGGCRHTTCCYSHSQTNISCPGCGDIILTSSGGRAVVSPNCEFKRIVRNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEA3</accession>
    <name>I7AEA3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264477.1">
        <property type="nucleotide sequence ID" value="XM_009266202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEA3"/>
    <dbReference type="GeneID" id="20521279"/>
    <dbReference type="KEGG" id="ero:EROM_050470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050470"/>
    <dbReference type="HOGENOM" id="CLU_022546_0_0_1"/>
    <dbReference type="OrthoDB" id="2188332at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="735" mass="84532" checksum="6C0457C696B2E8CA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEF8</accession>
    <name>I7AEF8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Skp1 G2 allele suppressor-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264547.1">
        <property type="nucleotide sequence ID" value="XM_009266272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEF8"/>
    <dbReference type="GeneID" id="20521351"/>
    <dbReference type="KEGG" id="ero:EROM_051190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051190"/>
    <dbReference type="HOGENOM" id="CLU_144307_0_0_1"/>
    <dbReference type="OrthoDB" id="1898560at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044563">
        <property type="entry name" value="Sgt1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45862">
        <property type="entry name" value="PROTEIN SGT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05002">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGS" evidence="2">
        <location>
            <begin position="66"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="17774" checksum="D119C8B6BB42199E" modified="2012-10-03" version="1">MEVAYDFAETKDSAIIFVYKPLAEDIQISPVLEDPRKLRLCKGIAIDLHKDVHPDIKLSESKSRIEIHLRKVETTRWGGINGKPSGKLQDGSKETTENDSEDEENTSVMDLLRKIYQRSGEDVRRAMEKSFYESEGTVLSTDWDQVKSKKITRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEH4</accession>
    <name>I7AEH4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_051400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264567.1">
        <property type="nucleotide sequence ID" value="XM_009266292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEH4"/>
    <dbReference type="GeneID" id="20521371"/>
    <dbReference type="KEGG" id="ero:EROM_051400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051400"/>
    <dbReference type="HOGENOM" id="CLU_110543_0_0_1"/>
    <dbReference type="OrthoDB" id="21380at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031431">
        <property type="term" value="C:Dbf4-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010571">
        <property type="term" value="P:positive regulation of nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901987">
        <property type="term" value="P:regulation of cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3410:FF:000001">
        <property type="entry name" value="Protein DBF4 homolog A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3410">
        <property type="entry name" value="DBF zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051590">
        <property type="entry name" value="Replication_Regulatory_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006572">
        <property type="entry name" value="Znf_DBF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038545">
        <property type="entry name" value="Znf_DBF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375">
        <property type="entry name" value="ACTIVATOR OF S-PHASE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15375:SF26">
        <property type="entry name" value="PROTEIN CHIFFON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07535">
        <property type="entry name" value="zf-DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00586">
        <property type="entry name" value="ZnF_DBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51265">
        <property type="entry name" value="ZF_DBF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DBF4-type" evidence="5">
        <location>
            <begin position="60"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="178" mass="20789" checksum="61D1B6FBA0D5DEC9" modified="2012-10-03" version="1">MKSYKVFKKPYILIEDCKNKCQPFYKEYVNSDAPKLHLDSPTLCCPFQAAKKYKQSGRRRHIREGFCEICYVRFNNYEEHIEEFEHREFAKDNSNYKKLDIFISTSAFENIGPPEEYIPQSPTFKLTPASSGRKYNGTCSNDNEKCSQTLHFSLISTEGCLGQDAMLFDHFIAEIFDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEU6</accession>
    <name>I7AEU6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_060770</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264667.1">
        <property type="nucleotide sequence ID" value="XM_009266392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEU6"/>
    <dbReference type="GeneID" id="20521473"/>
    <dbReference type="KEGG" id="ero:EROM_060770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060770"/>
    <dbReference type="HOGENOM" id="CLU_350220_0_0_1"/>
    <dbReference type="OrthoDB" id="2190858at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13145">
        <property type="entry name" value="SSM4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="238"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="327"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="581"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="622"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="664"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="753"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="15">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="180"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="809" mass="93671" checksum="9B241C51E5025189" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEV3</accession>
    <name>I7AEV3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Phospholipid-translocating P-type ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_060880</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264677.1">
        <property type="nucleotide sequence ID" value="XM_009266402.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEV3"/>
    <dbReference type="GeneID" id="20521484"/>
    <dbReference type="KEGG" id="ero:EROM_060880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060880"/>
    <dbReference type="HOGENOM" id="CLU_000846_3_1_1"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
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    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
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    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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    </dbReference>
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        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
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        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="772"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="805"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="853"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="889"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="913"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="958"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="9">
        <location>
            <begin position="5"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="10">
        <location>
            <begin position="742"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="990" mass="112917" checksum="7DB0E76788CDDE62" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF21</accession>
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        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
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        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Unikaryonidae</taxon>
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        </lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AFN83270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_389329_0_0_1"/>
    <dbReference type="OrthoDB" id="2193852at2759"/>
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    </dbReference>
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        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Sec8_exocyst_N"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF04048"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83270.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="709" mass="84192" checksum="58929A7DFCBACE11" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
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            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFC8"/>
    <dbReference type="GeneID" id="20521718"/>
    <dbReference type="KEGG" id="ero:EROM_071540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071540"/>
    <dbReference type="HOGENOM" id="CLU_116834_0_0_1"/>
    <dbReference type="OrthoDB" id="158360at2759"/>
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        <location>
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            <end position="193"/>
        </location>
    </feature>
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        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="194" mass="22423" checksum="E1C8782F6C517B85" modified="2012-10-03" version="1">MAEKDVPSLIEEENAIRKELKVCEEDLENIGALAIRIQSFSRSVSNLDPSEPEAERLSKQADLFSKSILRLGNQRSKKRSEMREEMTKSKANTEKEYMENTNIPQPISDDDFFSSSTKRINDVLMNAMDAFETVKRQNVYINRTSERIRASLSQIGLSKQLIDQIDKRYLSDRVLFMGGIVLLVIMFFLLRFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFD9</accession>
    <name>I7AFD9_ENCRO</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">EROM_071690</name>
    </gene>
    <organism evidence="3 4">
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="20521733"/>
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    <dbReference type="OrthoDB" id="10558207at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20096" checksum="981904CE2788D4AF" modified="2012-10-03" version="1">MWVIFLQGFQKLNLYIFIGTRIGEMYKCFEDSNCMKLPQLLIAASIMLLVTLIIIACEVLLNRSEESGIVKTKSRNNNAQDEHQTPAQRIIRALFRVITGMAVIKVIKGLLFPNNSKPDGENPESLPSAPPPYSAHPSHHLHQFPPHSSHQHYNFGHTNPLGYNSVVNQEHENFIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFE4</accession>
    <name>I7AFE4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">White ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_080020</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264922.1">
        <property type="nucleotide sequence ID" value="XM_009266647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFE4"/>
    <dbReference type="GeneID" id="20521738"/>
    <dbReference type="KEGG" id="ero:EROM_080020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080020"/>
    <dbReference type="HOGENOM" id="CLU_020421_1_0_1"/>
    <dbReference type="OrthoDB" id="2141921at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03234">
        <property type="entry name" value="ABCG_White"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF91">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="604"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="651" mass="75125" checksum="418FFA802F54A75F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFG6</accession>
    <name>I7AFG6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone H2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264952.1">
        <property type="nucleotide sequence ID" value="XM_009266677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFG6"/>
    <dbReference type="GeneID" id="20521769"/>
    <dbReference type="KEGG" id="ero:EROM_080330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080330"/>
    <dbReference type="HOGENOM" id="CLU_157385_0_0_1"/>
    <dbReference type="OrthoDB" id="2191729at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="2">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="13976" checksum="8A30E5938F926253" modified="2012-10-03" version="1">MAKSSRHVTGKAPGPALDGGDKKKTKKKSSSMCVGGMFKSAVKRISREVSPDNNIMLTSNSIQVLCGISFDFVDTVADLAGELARKVGKQTVGSDDIISAFEILLKGELRKLAIREVHNALSKSQAKSRGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFI2</accession>
    <name>I7AFI2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_080600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264977.1">
        <property type="nucleotide sequence ID" value="XM_009266702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFI2"/>
    <dbReference type="GeneID" id="20521796"/>
    <dbReference type="KEGG" id="ero:EROM_080600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080600"/>
    <dbReference type="HOGENOM" id="CLU_073901_2_0_1"/>
    <dbReference type="OrthoDB" id="10256606at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013238">
        <property type="entry name" value="RNA_pol_III_Rbc25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08292">
        <property type="entry name" value="RNA_pol_Rbc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase III subunit Rpc25" evidence="4">
        <location>
            <begin position="80"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20521" checksum="D92408BF249DCFA4" modified="2012-10-03" version="1">MFYTVEIEDRIKVSLESSLEKEREVFEELKRKYVGRVLEDGSICVLVVRVANVHEYKVCDEFLVARVEFEALLFKFFKYEVVCGTILEQNEDGIKLGVPFFSSLFVKKGDLPDMTEKVHVSDCGENQLAWVWIYRGNKLYFRRNACVRFRVLGCKEKSSLVLCSLGEAGLGPLEWWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFP7</accession>
    <name>I7AFP7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Palmitoyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_081490</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DHHC palmitoyltransferase family. PFA5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="11"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265062.1">
        <property type="nucleotide sequence ID" value="XM_009266787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFP7"/>
    <dbReference type="GeneID" id="20521884"/>
    <dbReference type="KEGG" id="ero:EROM_081490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081490"/>
    <dbReference type="HOGENOM" id="CLU_112124_0_0_1"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883:SF23">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="12">
        <location>
            <begin position="74"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="215" mass="24865" checksum="7E700D2482D7DB84" modified="2012-10-03" version="1">MEAWRMMNKLVGYTEILIVAFVIILYLLGFYEAIIVSGVSIRLYLLITSFYYYVLCLVKSPGQLLDFGNANVKGICKKCNRIVGTKTVHCEICNKCYYKRDHHCPIIGRCVASSNIKDLYLAVSFMGFHSLAAILRGSTRFAYMMSFHRYLFIMSSAFACWLTFLILTGKTTRELIRFQGRITDEIRMFRLKKIFRDGFVGTLAPYLRWKTTVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG38</accession>
    <name>I7AG38_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HEAT repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265202.1">
        <property type="nucleotide sequence ID" value="XM_009266927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG38"/>
    <dbReference type="GeneID" id="20564313"/>
    <dbReference type="KEGG" id="ero:EROM_090890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090890"/>
    <dbReference type="HOGENOM" id="CLU_595849_0_0_1"/>
    <dbReference type="OrthoDB" id="340346at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051023">
        <property type="entry name" value="PP2A_Regulatory_Subunit_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648:SF4">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2 (FORMERLY 2A), REGULATORY SUBUNIT A, BETA ISOFORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10648">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A 65 KDA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="459" mass="52638" checksum="3137A49F8A94888C" modified="2012-10-03" version="1">MISSEVSNLAENLKGQGHMSVEKLELLVGIDQKFNGLLVEHLAALPPDKSHLSICELLLFEEPEKTVPVIRRIFASFPSEVSKFIGKLLESPFYIQRQVIPLLIVNMEYKEEKEALRRCLRDSVVGVARCGALSLEKYNVIPFDDDELMEIVRDLHSSYSESIQCTSSYVIPLIKRKTQFVSEICALKSWRRRYAIAKRIPRLNVSDRAIIYSHLSQDPEEEIRICLASNMEYAEGWDRLVPLFLKDSSPTVRALMIQMVGDKEEFQEMLKEAVSDASWEVRKALLCVHRVDTYKSIVIPLINSLNSTPNWRIRKEILESIEYASKQDGKLLKMFLGKHLLGYLSDKVYEVRAEASRVVGELVMMYPWTIEWLPDIEAIVSSRSYLHRITSVNAALSFDKAHRTEFVKKLLSDPIENVKLYTLEMVDRHGMDEGIKEIVSGLCVSSDEEIRRAARNLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGH9</accession>
    <name>I7AGH9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Palmitoyltransferase</fullName>
            <ecNumber evidence="12">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_100590</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="12"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265372.1">
        <property type="nucleotide sequence ID" value="XM_009267097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGH9"/>
    <dbReference type="GeneID" id="20564489"/>
    <dbReference type="KEGG" id="ero:EROM_100590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100590"/>
    <dbReference type="HOGENOM" id="CLU_769870_0_0_1"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="343"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="63"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="96"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="13">
        <location>
            <begin position="269"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="366" mass="41744" checksum="4999B768A0CF7CA3" modified="2012-10-03" version="1">MDDEEIKDALMANNTKSLKAVPNNYKFSDGTGLIHWASMYNNLDMCRRMMECRGAANSVGGVLDSSPLYYALYNSNYKVMKFLIENGADIRYVNKSGLGLLHTCVRFDDVLGMILLLSYGADVNTKDTKNRTLWTYAKVKGARNVQRFLETNVVPGRYNEWGLEGFSIGMHGMSFFFGRTYQIGVIVMFLVFWGRIARTRAPMYLNLFYSFYIGCNVIEESCEHSVYALEYFFTLFRLFLSDPRFGSVSTYDKVKELVSTMIEQDEYMVENFCYTCLGRKGDGVKHCSICNRCVLEFDHHCPCINSCISKDIMGLFRRHLLSTFTVTVNVLLSGHASEVRMELVSVTVFIVLILAAGMDIRRPKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGJ3</accession>
    <name>I7AGJ3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265387.1">
        <property type="nucleotide sequence ID" value="XM_009267112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGJ3"/>
    <dbReference type="GeneID" id="20564504"/>
    <dbReference type="KEGG" id="ero:EROM_100740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100740"/>
    <dbReference type="HOGENOM" id="CLU_166317_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045176">
        <property type="entry name" value="Got1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493">
        <property type="entry name" value="CGI-141-RELATED/LIPASE CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21493:SF9">
        <property type="entry name" value="GOLGI TRANSPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="12921" checksum="098B75C3FD451F55" modified="2012-10-03" version="1">MSPKESGVVVMSIGMFVFLVGGILLMDRALMISGNLLIIIGISLLARSKMFTLLRPEKIQGMVIFVVAVLFLIYGFLAFGFLLEILGLFLLLRDNIPTFRAVLRTLLFGKLGRLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGR8</accession>
    <name>I7AGR8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_101650</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265477.1">
        <property type="nucleotide sequence ID" value="XM_009267202.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGR8"/>
    <dbReference type="GeneID" id="20564595"/>
    <dbReference type="KEGG" id="ero:EROM_101650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101650"/>
    <dbReference type="HOGENOM" id="CLU_446897_0_0_1"/>
    <dbReference type="OrthoDB" id="2196203at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="7">
        <location>
            <begin position="220"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="6">
        <location>
            <begin position="376"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="611" mass="71113" checksum="BD2918DCFE391EA3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGW3</accession>
    <name>I7AGW3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265537.1">
        <property type="nucleotide sequence ID" value="XM_009267262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGW3"/>
    <dbReference type="GeneID" id="20564657"/>
    <dbReference type="KEGG" id="ero:EROM_110580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110580"/>
    <dbReference type="HOGENOM" id="CLU_073878_0_0_1"/>
    <dbReference type="OrthoDB" id="2190427at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="326" mass="38553" checksum="CBA59002D3293DE9" modified="2012-10-03" version="1">MDLWVRNFLKIKQDHTDPSDYEKFFTCFIDYLARCPSSHCEVSKILVKALDTFKNVHLISIAYRLLYCVDVELQIPESTLKKHFRSPYLREYMFPLLPRISPGLFDSTLLKLLRNPHYECKNFFNLMKSKRDWVVEFLGLKRGHLARKQMMLMVHTSIFHNMGYFITFFGHRDRSLSFLAFEAFKLFVEGLVRERRGNGSEGEAVPILLHKEATFSIWWNYENAWISPEARIRVESPTNFLIHFMDFISEKEKLQRHISTNDKNGVEAILKKYIDLGIPLSIPDSRWTSEASRCPETPKRECVEDICFEYLFNGKTYKEIMDCIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGZ0</accession>
    <name>I7AGZ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265567.1">
        <property type="nucleotide sequence ID" value="XM_009267292.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGZ0"/>
    <dbReference type="GeneID" id="20564687"/>
    <dbReference type="KEGG" id="ero:EROM_110880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110880"/>
    <dbReference type="HOGENOM" id="CLU_146853_0_0_1"/>
    <dbReference type="OrthoDB" id="2191194at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="132" mass="15108" checksum="9985C6FABB9DECA6" modified="2012-10-03" version="1">MARRRYNILAEGCSDAKDSPAKHPDLSLTLESSLILSGTKYPEPRTRISFSFISTAESGVIKLFFVTITHSKSNSEYYGFDSSESCTIVSPDRLDVGKRYEIYGKLEHRSPRVLYCASIREICYRRLCYEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH79</accession>
    <name>I7AH79_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTPase-activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265637.1">
        <property type="nucleotide sequence ID" value="XM_009267362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH79"/>
    <dbReference type="GeneID" id="20564758"/>
    <dbReference type="KEGG" id="ero:EROM_111590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111590"/>
    <dbReference type="HOGENOM" id="CLU_005350_10_1_1"/>
    <dbReference type="OrthoDB" id="294251at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000016">
        <property type="entry name" value="TBC1 domain family member 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF9">
        <property type="entry name" value="GTPASE ACTIVATING PROTEIN AND CENTROSOME-ASSOCIATED, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="75"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="320" mass="37657" checksum="80DEEA874BE88706" modified="2012-10-03" version="1">MKNERCIRELYNTSGMSEEVFCTNEYGFVVDAFNSQPKDSHISIKNEWYDLIARCRNNGEKLRKSEGARKLIYQGIPLSLKYRLWGLFTQKASGFNYSNLISKKSGYEHQIHVDVQRTFRRHFLFNKEYGRGQCELFNILTAYSNYNPEVGYCQGMSSVAALLLMYFPEEEAFEMLVSIIKNNNLEALFDKKLSKVPRVQRIQDEVFKALIPEVYGHLLHQNIDIGVYAVGWYLTLFTRFDIKLVLRMWDFFLFFDFSAFIFFAAGILKFFARKILELQGEQLIEFIGTLDSKEVDVEKVVSYVVESFRNGEYEYYRDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH84</accession>
    <name>I7AH84_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription initiation factor TFIID subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_111640</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265642.1">
        <property type="nucleotide sequence ID" value="XM_009267367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH84"/>
    <dbReference type="GeneID" id="20564763"/>
    <dbReference type="KEGG" id="ero:EROM_111640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111640"/>
    <dbReference type="HOGENOM" id="CLU_005884_2_1_1"/>
    <dbReference type="OrthoDB" id="10266330at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08044">
        <property type="entry name" value="TAF5_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.500">
        <property type="entry name" value="TFIID subunit TAF5, NTD2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007582">
        <property type="entry name" value="TFIID_NTD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037264">
        <property type="entry name" value="TFIID_NTD2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879:SF1">
        <property type="entry name" value="CANNONBALL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19879">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04494">
        <property type="entry name" value="TFIID_NTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160897">
        <property type="entry name" value="Taf5 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TFIID subunit TAF5 NTD2" evidence="8">
        <location>
            <begin position="16"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="360"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="402"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="444"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="486"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="556" mass="63212" checksum="A8D931728785CD13" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL62</accession>
    <name>I7AL62_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="11">PAGM</shortName>
            <ecNumber evidence="4 11">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">EROM_010440</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, which is a biosynthetic precursor of chitin and also supplies the amino sugars for N-linked oligosaccharides of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11 14">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 11"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263886.1">
        <property type="nucleotide sequence ID" value="XM_009265611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL62"/>
    <dbReference type="GeneID" id="20520671"/>
    <dbReference type="KEGG" id="ero:EROM_010440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010440"/>
    <dbReference type="HOGENOM" id="CLU_022890_1_0_1"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000013">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="16">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="16">
        <location>
            <begin position="122"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="18">
        <location>
            <begin position="202"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="17">
        <location>
            <begin position="297"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="15">
        <location>
            <begin position="456"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="12">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="14">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="369"/>
            <end position="371"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="481"/>
            <end position="485"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AFN82389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="530" mass="58721" checksum="B1A496BFA4B41D16" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALD0</accession>
    <name>I7ALD0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Glutamyl aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_011300</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263971.1">
        <property type="nucleotide sequence ID" value="XM_009265696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALD0"/>
    <dbReference type="GeneID" id="20520757"/>
    <dbReference type="KEGG" id="ero:EROM_011300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011300"/>
    <dbReference type="HOGENOM" id="CLU_003705_0_3_1"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000006">
        <property type="entry name" value="Puromycin-sensitive aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF299">
        <property type="entry name" value="AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 13" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="12">
        <location>
            <begin position="262"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="584" mass="66524" checksum="25CD7DE3C725CB73" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALM9</accession>
    <name>I7ALM9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_021040</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264076.1">
        <property type="nucleotide sequence ID" value="XM_009265801.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20520866"/>
    <dbReference type="KEGG" id="ero:EROM_021040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021040"/>
    <dbReference type="HOGENOM" id="CLU_241340_0_0_1"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF69">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="13">
        <location>
            <begin position="931"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="12">
        <location>
            <begin position="1499"/>
            <end position="1798"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="14">
        <location>
            <begin position="1778"/>
            <end position="1810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1810" mass="210187" checksum="87E6E753D4D71B3C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALY3</accession>
    <name>I7ALY3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_030380</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009265881.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20520949"/>
    <dbReference type="KEGG" id="ero:EROM_030380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030380"/>
    <dbReference type="HOGENOM" id="CLU_093932_3_0_1"/>
    <dbReference type="OrthoDB" id="282152at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
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        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006386">
        <property type="term" value="P:termination of RNA polymerase III transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1 5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="9">
        <location>
            <begin position="60"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="11901" checksum="F2B0EDF8E24B72D9" modified="2012-10-03" version="1">MLFCPLCSSMLVVKRQPVGNELSCRMCGYLYAISKEITKATPMTPKKNDGLIDEDDNLKFVSKCGKRCECGSEEVSFVELQTRSADEPMTIFYKCIRCKKVWRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMK1</accession>
    <name>I7AMK1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2193951at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="12374" checksum="23D4EA0A0DA05100" modified="2012-10-03" version="1">MFLSYSRMKICLHNLYFVLILMVFTLINCRGLHVHDLNSKSYRHDELRGWKHPEFRRGLYMDRTHVPVYGGRGIITLEDLKRPNVPVIVPYEVTPKQVLTLLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="1">T-complex protein 1 subunit zeta</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">EROM_060450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264636.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN40"/>
    <dbReference type="GeneID" id="20521442"/>
    <dbReference type="KEGG" id="ero:EROM_060450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060450"/>
    <dbReference type="HOGENOM" id="CLU_1906721_0_0_1"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="133" mass="14847" checksum="1279B3AC1EAE8F66" modified="2012-10-03" version="1">MSRMESGIRGALKSLYISLKNRKYIEGGYSLYRSLISYIREKMNSVSEKDVVGYKVMENSFLNVVKALLRNSGKDIQEELTRILRGGECERVVDNSCVVSAVISNSTVVATSLLLVDEIIKAGKPIKENKPEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN72</accession>
    <name>I7AN72_ENCRO</name>
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        <submittedName>
            <fullName evidence="7">Pre-mRNA splicing helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_060870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83179.1"/>
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    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17921">
        <property type="entry name" value="DEXHc_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF6">
        <property type="entry name" value="DNA-DIRECTED DNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
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    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="253"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="445"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1480" mass="169179" checksum="9290F6C1DCE3CAE0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANF0</accession>
    <name>I7ANF0_ENCRO</name>
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        <recommendedName>
            <fullName evidence="5">PUM-HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070430</name>
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    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264791.1">
        <property type="nucleotide sequence ID" value="XM_009266516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANF0"/>
    <dbReference type="GeneID" id="20521602"/>
    <dbReference type="KEGG" id="ero:EROM_070430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070430"/>
    <dbReference type="HOGENOM" id="CLU_051716_0_0_1"/>
    <dbReference type="OrthoDB" id="2191363at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF13">
        <property type="entry name" value="PUMILIO HOMOLOGY DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="327"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="444" mass="51152" checksum="B75C4D813B1E29DA" modified="2012-10-03" version="1">MNLAIEEPSKVIKMRVNGAWPSRQSRHDRAVSHIKKKESSSLYGEKLVESIAQKRAAFDPCDEENCPDGAMFNTKEVSIDFFDMNSVERKQQRECKVESEGTKEALHPYGYEIGEGILERSFIEGMLENDIRVKDIKYGEDLIFIYFFTVEDSISFYRQFLPYVEIHFSETYDFEQAVLLSSSEWANIALLSMHNSSKENYTCSSSFNEWLKTSNSLESKFPKHEIHRHTKDKSLAFFHNTDPEFGEWDGELSERNLFERKIEHFTHLRNFFSRSEVRIMIQSLEMGNVDFVFKNTKELAVGNQSNKVMQEAIGLLPDAGIARIIQNLGHDIAPIAANKHGAYTIQMLISVSKTPLTQNLLSKYFRKFGGFLITHEIGNYAIQKILRFDPDLVFELFISNIGDIVKSELGIKVLRRCLEFFPDKKAILAQKVREHGKNLSVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANI9</accession>
    <name>I7ANI9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">60S ribosomal protein L24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264836.1">
        <property type="nucleotide sequence ID" value="XM_009266561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANI9"/>
    <dbReference type="GeneID" id="20521648"/>
    <dbReference type="KEGG" id="ero:EROM_070880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070880"/>
    <dbReference type="HOGENOM" id="CLU_150146_0_0_1"/>
    <dbReference type="OrthoDB" id="1727108at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL24-related N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="100" mass="11416" checksum="F7176B4DE6E93B12" modified="2012-10-03" version="1">MIEGVSVFSGHNIPKGSGIYRVMNDGRTVLTASNKERKLIQRKINPRDIRWTTTSREFFGKESQYVVEKMNVQKVKIVRGFRHISASSFEKARQAGSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANJ4</accession>
    <name>I7ANJ4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">2-(3-amino-3-carboxypropyl)histidine synthase subunit 1</fullName>
            <ecNumber evidence="3 11">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_070930</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the DPH1/DPH2 family. DPH1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 11"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264841.1">
        <property type="nucleotide sequence ID" value="XM_009266566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANJ4"/>
    <dbReference type="GeneID" id="20521653"/>
    <dbReference type="KEGG" id="ero:EROM_070930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070930"/>
    <dbReference type="HOGENOM" id="CLU_037146_1_1_1"/>
    <dbReference type="OrthoDB" id="1649088at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="347" mass="39676" checksum="4E4E914A86921E2D" modified="2012-10-03" version="1">MVVLEQKEIPESRMYLPSNYNFEMAKVVKTIGRLNAKKVTLQFPDGLLKYSFVIIDIIEKYTDAECVILNDVVYGGCCVDDESIDSDLLVHYGHSCLVPVGEMNTKVLYVFVDIRIDIDHAAEMIRRNFEGKIAVIGTIQYNSSINKLRRVLSEEKDGVECILPQVRPLSSGEVLGCTSPRIEGVSAVVSIGDGRFHLEGAMIRNPHLKFYKYCPFNRRMTQESYDYHTMISARRSAIGRALNGRSFGVILGSLGRQGNRSILRSVVDKLKKYDIYLLMLDEISPKKLERYDFIDSFVQISCPRLSIDWGTLFKKPLLSPFEVFYQGGEYLMDYYSKEGKGEWKNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANL1</accession>
    <name>I7ANL1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 16">Tubulin alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">EROM_071130</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 16">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264861.1">
        <property type="nucleotide sequence ID" value="XM_009266586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANL1"/>
    <dbReference type="GeneID" id="20521673"/>
    <dbReference type="KEGG" id="ero:EROM_071130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071130"/>
    <dbReference type="HOGENOM" id="CLU_015718_0_0_1"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02186">
        <property type="entry name" value="alpha_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000001">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000007">
        <property type="entry name" value="Tubulin alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002452">
        <property type="entry name" value="Alpha_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01162">
        <property type="entry name" value="ALPHATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="17">
        <location>
            <begin position="46"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="18">
        <location>
            <begin position="244"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AFN83364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="441" mass="49473" checksum="CCC8A6E8611D9E88" modified="2012-10-03" version="1">MREIISLHIGQAGVQIGNACWELYCKEHGILPDGRLDQNRMDDESAESFFSQTSVGTYVPRTLMVDLEPGVLESIKTGKYRELYHPGQLISGKEDAANNYARGHYTVGKEIIEPVMEQIRRMADNCDGLQGFLIYHSFGGGTGSGFASLMMDRLAAEFGKKSKLEFSVYPAPKIATAVVEPYNSILTTHTTLDYSDCSFLVDNEAIYDMCRNLGIQRPYYTDINRIIAQVVSSITASLRFPGSLNVDLTEFQTNLVPYPRIHFPLVAYSPMLSKEKAAHEKLSVQEITNACFEPQSQMVRCDTRKGKYMACCLLFRGDVNPKEANNATANVKAKRTNQFVEWCPTGFKVGINSRKPTVLDGEAMAEVSRAVCALSNTTAISEAWKRLNNKFDLMFSKRAFVHWYVGEGMEEGEFSEAREDLAMLEDDYERISSNAEPIDEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANL6</accession>
    <name>I7ANL6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264866.1">
        <property type="nucleotide sequence ID" value="XM_009266591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANL6"/>
    <dbReference type="GeneID" id="20521678"/>
    <dbReference type="KEGG" id="ero:EROM_071180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071180"/>
    <dbReference type="HOGENOM" id="CLU_2622027_0_0_1"/>
    <dbReference type="OrthoDB" id="2190729at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="78" mass="9369" checksum="0D2A05BAA6E4763C" modified="2012-10-03" version="1">MLSIDDIIGEKDLPRLFRTVVKKREATTPDKQCVIRRIREFGPLTMESLEGLYDEDILTELERSRRWSILRRVKKDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP72</accession>
    <name>I7AP72_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome regulatory subunit 6B homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_081950</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265106.1">
        <property type="nucleotide sequence ID" value="XM_009266831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP72"/>
    <dbReference type="GeneID" id="20521930"/>
    <dbReference type="KEGG" id="ero:EROM_081950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081950"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_0_1"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="178"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="17"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="398" mass="44930" checksum="D07951B47FA64822" modified="2012-10-03" version="1">MLINHRVCSPLMCGGLKADLFVRYKQMERKLKLLEIREAYVRKEIETLKREERHGREELDRVKSVPLIMGQFLEPIDNSTAIVGSTAGSNFVVRILSTVDRELLRPNTTVALHRHSSAIVDVLPPEVDSTIPVMGEEEKPDVTYADVGGLDVQKQEIRETVELPLLQSDLYRQIGIDPPQGVLLYGPPGTGKTMLVKAVANHTKATFIKVNGSEFVQKYLGEGPRMVRDVFRLAREKAPSIVFIDEVDSIATKRFDASTSADREVQRVLIELLNQMDGFDQTANVKVIMATNRADTIDPALLRPGRLDRKIEFPLPDRRQKRLVFNAITSKMSLKDEVDIESLVCRPERISCADINSICQEAGMLAVRASRYMVTQKDFEEAYSKVVERSDTRPAFYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7APC5</accession>
    <name>I7APC5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_090570</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265171.1">
        <property type="nucleotide sequence ID" value="XM_009266896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APC5"/>
    <dbReference type="GeneID" id="20564281"/>
    <dbReference type="KEGG" id="ero:EROM_090570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090570"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="12">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="69"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="252"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="38"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="425" mass="48342" checksum="A5A894D94AD74BE8" modified="2012-10-03" version="1">MKQALEQVEDFENLKGSNAQIKDFEDLRSDSSQIRMFDTWEDYGLKEDLLKGIYGIGFETPSFIQKAAIQPIIDGRDIRAQAQSGTGKTGAFAVAALQMCDTNQDTTQILVLASTREIAAQNAARFEDLGCFMGAGVALLSGGSPISADKAALEKNPHIVVGTPGRVEHMININELNMDNIKLFVIDEADEMLKAGFQEQVKSIFRRITNKDEVQIAMFSATYDEEELRVSEEILINPVIIDLRYNDQTLKGIRQYFIDLKKEPPFRKGREDYLLPKLVTLYDIFRKQRLGQSIVFINSKEDARIVYDWLIKHEWECELISAELTQAERERTLNRFRGGTARCLISSGLLSRGIDIQNLSVVFCLDVPSFERKSTYIHRIGRSGRYGRKGIAINIVYEYELKNLKAIERFYNTTIKELPADFSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ96</accession>
    <name>I7AQ96_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263984.1">
        <property type="nucleotide sequence ID" value="XM_009265709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ96"/>
    <dbReference type="GeneID" id="20520770"/>
    <dbReference type="KEGG" id="ero:EROM_020120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020120"/>
    <dbReference type="HOGENOM" id="CLU_1415145_0_0_1"/>
    <dbReference type="OrthoDB" id="2194473at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="133"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="192" mass="22405" checksum="A2BD009D7A9497C5" modified="2012-10-03" version="1">MEEDGPRLAKMRQAYKRAIQEILKEKEKIKGILTDPNTLCEDSFFMDSSKAGETHQRDPEETSSAIENIFQGLRSKLSDVFRKKLEMNDISNKLNRLDRDVLEGRTSLRDVTSKEYVREIFESYLVNTKVDYIDYIEETKREALERIRVLKNELEKATEELGLLKRENTLCRNTYDNLISSFSKAARNKNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQA4</accession>
    <name>I7AQA4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">U2 snRNP spliceosome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_110770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SF3B1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265556.1">
        <property type="nucleotide sequence ID" value="XM_009267281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQA4"/>
    <dbReference type="GeneID" id="20564676"/>
    <dbReference type="KEGG" id="ero:EROM_110770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110770"/>
    <dbReference type="HOGENOM" id="CLU_321319_0_0_1"/>
    <dbReference type="OrthoDB" id="2196406at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038737">
        <property type="entry name" value="SF3b_su1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12097">
        <property type="entry name" value="SPLICING FACTOR 3B, SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="902" mass="102253" checksum="12F8054706693C11" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQC3</accession>
    <name>I7AQC3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Golgin subfamily A member 7/ERF4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110970</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265576.1">
        <property type="nucleotide sequence ID" value="XM_009267301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQC3"/>
    <dbReference type="GeneID" id="20564696"/>
    <dbReference type="KEGG" id="ero:EROM_110970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110970"/>
    <dbReference type="HOGENOM" id="CLU_167756_0_0_1"/>
    <dbReference type="OrthoDB" id="2190159at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019383">
        <property type="entry name" value="Golgin_A_7/ERF4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10256">
        <property type="entry name" value="Erf4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Golgin subfamily A member 7/ERF4" evidence="3">
        <location>
            <begin position="8"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="118" mass="13534" checksum="3B14174FDAA27E45" modified="2012-10-03" version="1">MTRKTVCIQIPRDPRTDGISFITSMPEIMAEKIERQKWKEIISGLNDVLYGLESPSLVSFIKTVSIIPFLVSTPQNAYVRVEEYLSEANKLLERYGIRIIHPGHHQYVELEVEICQSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQH9</accession>
    <name>I7AQH9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protein prenyltransferase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_020970</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein prenyltransferase subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264069.1">
        <property type="nucleotide sequence ID" value="XM_009265794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQH9"/>
    <dbReference type="GeneID" id="20520858"/>
    <dbReference type="KEGG" id="ero:EROM_020970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020970"/>
    <dbReference type="HOGENOM" id="CLU_885955_0_0_1"/>
    <dbReference type="OrthoDB" id="2192310at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.120">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002088">
        <property type="entry name" value="Prenyl_trans_a"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11129">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE ALPHA SUBUNIT/RAB GERANYLGERANYL TRANSFERASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01239">
        <property type="entry name" value="PPTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48439">
        <property type="entry name" value="Protein prenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51147">
        <property type="entry name" value="PFTA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="386" mass="46400" checksum="671CF734519F1298" modified="2012-10-03" version="1">MSFTSFFVTKNSFPPDLTLRHFFVGLLPRNLSIRSILSKGTLFEANTILRRINLWRKETNPRMIIHNVEEGEYIEDNDAFDRLLKLRSKEESIEKHKEIVQLVSDDYFSWNKLKEYLLANPSDFRSQLKVCENSLRSNPKSYQPWHHRKFMMKNFQRQREKYLDREDFLTKLLLDSDPRNFHCWNYRMSILNTKTGRDLFNYSYLHHHQDSEDPLSIIYTDPLDPTSWEYFYLWRERKRMRNGLYIRRYKDHLEIRFSKPFQGQIIFEADKTRKIVASELDTRIIVIEETMGALDSFWVIMNGETVYLSLEDEGFGFVHEILEVEPECCGALLMLLDYIKEETERISIIEKIISLDPIKKNYYNTLCGRFYSVYVPEPEKYFKNKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQK5</accession>
    <name>I7AQK5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">proline--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_021270</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="2"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264099.1">
        <property type="nucleotide sequence ID" value="XM_009265824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQK5"/>
    <dbReference type="GeneID" id="20520889"/>
    <dbReference type="KEGG" id="ero:EROM_021270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021270"/>
    <dbReference type="HOGENOM" id="CLU_001882_4_1_1"/>
    <dbReference type="OrthoDB" id="1350766at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00862">
        <property type="entry name" value="ProRS_anticodon_zinc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.30:FF:000001">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000007">
        <property type="entry name" value="Bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000005">
        <property type="entry name" value="bifunctional glutamate/proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016061">
        <property type="entry name" value="Pro-tRNA_ligase_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09180">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00946">
        <property type="entry name" value="ProRS-C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="47"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="511" mass="57873" checksum="D7779E7381F43E6B" modified="2012-10-03" version="1">MNIISFSCPIMTKQKFGLTVKKEEDFSEWYVQVIIKGEMIDYYEIKGCYVMRPQGQFIWKCIQKWFTEKIEEAGVQECYFPMLIPKSMLEKEKSHIENFSPEVAWITKCGNEVLGDPVAIRPTSETIIYPSFSKWIRSHRDLPLKINQWCSVLRWELHGTLPFIRGKEFLWQEGHTAFLTKEESDEEVLAILDLYSRIYEELLAVPVIKGRKTESEKFGGADYTTSIEAFIPGSGRGVQAATSHFLGQNFSRMFDIKVDTDEGSENSSFVYQNSWGITTRSIGIAVMIHSDNLGLVLPPRVAMTQVVIIPCGITSSSSKDDAERLKMYIGKIQAQLKSSGIRVYVDDRNNVTVGFKFNHWEIRGVPLRLEVGFKDMEGAEACLVRRDTRAKRQISVEGIDQTVANEIDTMHNDMLARAISERDSRISYAKNFEDFISALDNKNIIMAPWCGIDECESTIKSRSTKLGPNGSVISTGAKTLCIPYDSKPCSGLECVSCGKQAIHYTLFGRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQM6</accession>
    <name>I7AQM6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265656.1">
        <property type="nucleotide sequence ID" value="XM_009267381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQM6"/>
    <dbReference type="GeneID" id="20564777"/>
    <dbReference type="KEGG" id="ero:EROM_111780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111780"/>
    <dbReference type="HOGENOM" id="CLU_1366628_0_0_1"/>
    <dbReference type="OrthoDB" id="2191933at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22617" checksum="9CE0E5773B9C57A5" modified="2012-10-03" version="1">MQKKIKASIVNGMLIIMIVGLGGVITHLSKDYNFFGAVSEDTKNIILMNKRDIEKDIYGDRMKIKIPNRSLCKGMWKGFEDLKTNAIIIISQDEVVKIFNIVSKEMKEYSYDVFIEHINSKGGRLSFLNNAKHGIRNFLKDTFENDERYITEDAPIVYGCLIVPNTDGGLKFMSKSKVMDVDCNWGDIIEYICPDEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQM9</accession>
    <name>I7AQM9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">U3 small nucleolar ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265661.1">
        <property type="nucleotide sequence ID" value="XM_009267386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQM9"/>
    <dbReference type="GeneID" id="20564782"/>
    <dbReference type="KEGG" id="ero:EROM_111830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111830"/>
    <dbReference type="HOGENOM" id="CLU_127204_0_0_1"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Brix" evidence="1">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="182" mass="21319" checksum="0CD86C0F9E84ECD9" modified="2012-10-03" version="1">MRVSVSVPPNPHKNTKILLKEFLDIIPNSYANDTDKESEVSIRIVEDVGPQWIVFKNSQLQLVLKIVQYKSRDYLRVSKPISKDHPPQLIVNNFTTDIGMKIVDFLANMFPFNQSSRQVANFTVQGDFIYFRLYKYCFGEKNPIMENVGPHLTLRLWRMIEYNGNEKKVMNFKKFIKNSNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQV5</accession>
    <name>I7AQV5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264184.1">
        <property type="nucleotide sequence ID" value="XM_009265909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQV5"/>
    <dbReference type="GeneID" id="20520977"/>
    <dbReference type="KEGG" id="ero:EROM_030660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030660"/>
    <dbReference type="HOGENOM" id="CLU_276236_0_0_1"/>
    <dbReference type="OrthoDB" id="2191583at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1115" mass="129691" checksum="8ED007B4538512D2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR67</accession>
    <name>I7AR67_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication licensing factor MCM2</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_040790</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264344.1">
        <property type="nucleotide sequence ID" value="XM_009266069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR67"/>
    <dbReference type="GeneID" id="20521143"/>
    <dbReference type="KEGG" id="ero:EROM_040790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040790"/>
    <dbReference type="HOGENOM" id="CLU_000995_0_1_1"/>
    <dbReference type="OrthoDB" id="844at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001141">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008045">
        <property type="entry name" value="MCM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF44">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01658">
        <property type="entry name" value="MCMPROTEIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="378"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN82847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="784" mass="88810" checksum="DBF32A8208E498C2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR89</accession>
    <name>I7AR89_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UDP-Gal beta GlcNAC beta 1,4 galactosyltransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266099.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR89"/>
    <dbReference type="GeneID" id="20521174"/>
    <dbReference type="KEGG" id="ero:EROM_041100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041100"/>
    <dbReference type="HOGENOM" id="CLU_1594519_0_0_1"/>
    <dbReference type="OrthoDB" id="2192533at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="167" mass="18969" checksum="E18D6C29D1C1D10F" modified="2012-10-03" version="1">MIYTKEVYESVKKLLGDLCMAQSRDAGVAYRVSKYKSKDPVFLVEGNGGEMYLMEDGAFELESRGFDIEVPKEKEVTVVFFRFSTKSPARNMTADKPFSDGHYMEVDAGPVTWDGRRIGTLITSRIPQSLYALSSVLRRFIRINGEENRNETFERSLVNSLSSNKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARA4</accession>
    <name>I7ARA4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264394.1">
        <property type="nucleotide sequence ID" value="XM_009266119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARA4"/>
    <dbReference type="GeneID" id="20521194"/>
    <dbReference type="KEGG" id="ero:EROM_041310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041310"/>
    <dbReference type="HOGENOM" id="CLU_1102771_0_0_1"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63825">
        <property type="entry name" value="YWTD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="252" mass="28084" checksum="AFDCC92CD0D084A6" modified="2012-10-03" version="1">MNLISATNGNIVFHKNKTLVVRDIATGAIEKIDAPSRISFMSGKYLADGEKNLHFIEGPTLRNVLKLNRCIFCLVEYENEVYVADRFGDVHRIRGERYEYVLGTLSYLTGMAIHNGRILLSDKYGRIRISGMDGKILGYRFGCGPIVSLECINGALVSISNKQIVLYDKEYSSSFIFELPEGTSVVKSMGKGKDELVVICSDSYFLFRIGDGIALVSHIEETIIDGVCSGSVFYKVLPNFTIVDGNEKVFYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARB4</accession>
    <name>I7ARB4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="12">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_041410</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="12"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264404.1">
        <property type="nucleotide sequence ID" value="XM_009266129.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521204"/>
    <dbReference type="KEGG" id="ero:EROM_041410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041410"/>
    <dbReference type="HOGENOM" id="CLU_000487_2_4_1"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02735">
        <property type="entry name" value="RNAP_I_Rpa1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01435">
        <property type="entry name" value="RNAP_I_RPA1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047107">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015699">
        <property type="entry name" value="DNA-dir_RNA_pol1_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="14">
        <location>
            <begin position="251"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1113"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1130"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1167"/>
            <end position="1182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1396" mass="156835" checksum="1709C56AB04EEEF6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARF7</accession>
    <name>I7ARF7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-directed RNA polymerase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_050240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264454.1">
        <property type="nucleotide sequence ID" value="XM_009266179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARF7"/>
    <dbReference type="GeneID" id="20521256"/>
    <dbReference type="KEGG" id="ero:EROM_050240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050240"/>
    <dbReference type="HOGENOM" id="CLU_090381_5_1_1"/>
    <dbReference type="OrthoDB" id="10248581at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF16">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="4">
        <location>
            <begin position="19"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="96" mass="11063" checksum="566EA0E9BDCCD325" modified="2012-10-03" version="1">MATARKVTMNYAGNTRNTMELKIEGETHTLGNLLCEEMLEDKRCLFSAYRVEHPTDNHVFLRITADKDCQVRDLLLETLKRIEEDTVSLMGQLKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARJ1</accession>
    <name>I7ARJ1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spore wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264489.1">
        <property type="nucleotide sequence ID" value="XM_009266214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARJ1"/>
    <dbReference type="GeneID" id="20521291"/>
    <dbReference type="KEGG" id="ero:EROM_050590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050590"/>
    <dbReference type="HOGENOM" id="CLU_1219685_0_0_1"/>
    <dbReference type="OrthoDB" id="2190493at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707602" description="Spore wall protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="245" mass="27515" checksum="E372C1AF9F8FFC18" modified="2012-10-03" version="1">MNAISSLVLLGWAVCLENAAKSFDPVLPSTTFYKPDAMSTESKYSLLTSKPEVPNKNTSLQSPVAFGEGSFYKLLHPSMTRLTDYLNSHSTGFSEWYLSPSLIMNKDLRDEIVSDLYRVQPQGDKELKEFWGSIMGELLIRDLPELKSSLGELKEIQNEERKKKSIAELKDINNKKIDEEDNFFGTAWFILDCLSLGISNRNAEIYTDVIGPFLDAAVGFKTLEHNFSNIIGSSRSITSHKTSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARR2</accession>
    <name>I7ARR2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_051370</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264564.1">
        <property type="nucleotide sequence ID" value="XM_009266289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARR2"/>
    <dbReference type="GeneID" id="20521368"/>
    <dbReference type="KEGG" id="ero:EROM_051370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051370"/>
    <dbReference type="HOGENOM" id="CLU_013159_0_0_1"/>
    <dbReference type="OrthoDB" id="2195965at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF0">
        <property type="entry name" value="SMP-LTD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SMP-LTD" evidence="9">
        <location>
            <begin position="60"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="958" mass="110911" checksum="00480FBCF7CF4A29" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS46</accession>
    <name>I7AS46_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pseudouridine synthase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_061200</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264709.1">
        <property type="nucleotide sequence ID" value="XM_009266434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS46"/>
    <dbReference type="GeneID" id="20521516"/>
    <dbReference type="KEGG" id="ero:EROM_061200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061200"/>
    <dbReference type="HOGENOM" id="CLU_005281_3_0_1"/>
    <dbReference type="OrthoDB" id="447290at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="4">
        <location>
            <begin position="284"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="487" mass="56447" checksum="D60568076DAA4F69" modified="2012-10-03" version="1">MDDFESIGVRRFFNGHIENAECVLKQEAFDFVVQEITDSRVCGVYPIVDYKKFLDGKEVAGYAEGLRSEEKEYRSMIHKKLKYYPFVKAKTVNGEIRLENSDVSVYSFVLQKVMFNTIDAGRWICRRLGVPENAFQFAGNKDKRAVTFQEVTIRCSFMSLYKLAVELNEEANCFEWEDFIVEGYTRERIENENKMIEKEILMTVGISFEEWKKGLKGKEDEVKERCEKNRREDPMGWEKVRSSIKIFDIKKSAAKNLGDLKGNRFSIALKGFRDQGLVKNLERGFLNYFGQQRFGNKLRNHEIGYDILTRRYEEAIDKIMKGGKCHELYISGKYEEAMGLGDSVEKYILKCKSKGMKEKDMVYGLRRELRMLYLHSYQSLKFNEAINSRMELGTDVVEGDMVYVNGDIKSVKDPLEFSIFDVVLPLEKFDNKMLKGGYRKVVEKADDVVISDAKDGIVVRFSLNSSCYATMALREVIGNSVLNRIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS55</accession>
    <name>I7AS55_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cell division control Cdc23-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_061410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264729.1">
        <property type="nucleotide sequence ID" value="XM_009266454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS55"/>
    <dbReference type="GeneID" id="20521538"/>
    <dbReference type="KEGG" id="ero:EROM_061410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061410"/>
    <dbReference type="HOGENOM" id="CLU_018320_5_0_1"/>
    <dbReference type="OrthoDB" id="10262026at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF10">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="266"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="334"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="2">
        <location>
            <begin position="368"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="463" mass="54215" checksum="052BFC8CF8582E20" modified="2012-10-03" version="1">MMAEFFTRNLHKTVDFLSKVGITSDEISVTLSSREYLSLTYQNLKEYRKAYASLLCKTSLLHLEEIVFCACCSQRINFIRNINLLIHLNKPVKIHWCGCEKDEFLLYIEGKVKRDFRILSDVVVINPWFWEAYSDLAELATPEIIESINIVGGLSDFFFMYLFCTKMIKKAWSPAMISEDEGRGSKWMKSSDGLCVNMKYPNLAGAVLYHQKDFDGCIEIFEKVTRNSFYYDLDYIDLYSNALYIKNDSRVILLAENTLNINKYRSETMCCIANYYSMKKEHEKAIEYFRLCVKLNPSSSIVHTLIGHEYLEMKSMEKAVSSYNVALKMCPMDYRAWYSIGQAYVTMTMYEYALFFIKKALECKNNDPVVWTTLGQCYMNLNRMDDAIGCFKNVIELNDPDGYLYIGDAYKNMKMYTEAVVYYEKYVETSKDDTRKICLFLEEYFKRMFDDKRSSYYAGLANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS97</accession>
    <name>I7AS97_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264794.1">
        <property type="nucleotide sequence ID" value="XM_009266519.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS97"/>
    <dbReference type="GeneID" id="20521605"/>
    <dbReference type="KEGG" id="ero:EROM_070460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070460"/>
    <dbReference type="HOGENOM" id="CLU_000288_126_3_1"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051726">
        <property type="entry name" value="Chitin_Synth_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430:SF1">
        <property type="entry name" value="CHITIN SYNTHASE REGULATOR SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46430">
        <property type="entry name" value="PROTEIN SKT5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="399" mass="44434" checksum="D01C719F8E42BAAB" modified="2012-10-03" version="1">METAAKSVSMGGIGEEIVGGSQDDTEEDVSGALVALGHYVRKMEDERGDSKKVFPGNSLLMEKALRYLTEKQCLSIKGVLDKCSSSHMAQPLNLTSQPEMHYYLGLSYEKGLFGMRRDYRKAFGHYLTSAQLGSPIGTFKVAQCYEKGIGKKRSIKNALCFYRCAAKLGLVDAMHTYGVIILFGNIENDCDLETGYFYLRLAARKATYTYPYALYDLARCYERGKGPDIVSPDDSYAFKLYLRGGSLDCPNCQFRIGKCFENGDMGQEKDMVKALEWYAKAADLGQPDAQLRLSALFLNGFEDIVKRNHGLAFKLGLKAATRENVSAAYLVSECYEQGIGIKRNALLALWWGRIAEELRIDQGNLPAERIRIIVPEEEDTSVEHLDFEDGAVPGLIKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASD1</accession>
    <name>I7ASD1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264834.1">
        <property type="nucleotide sequence ID" value="XM_009266559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASD1"/>
    <dbReference type="GeneID" id="20521646"/>
    <dbReference type="KEGG" id="ero:EROM_070860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070860"/>
    <dbReference type="HOGENOM" id="CLU_1427969_0_0_1"/>
    <dbReference type="OrthoDB" id="2188105at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="195" mass="22504" checksum="62446B20FA7687D2" modified="2012-10-03" version="1">MGNPSMPVIILLGRSKMYLEELSTYLSAHFRVVHLDDEFRIIRSKLSNMDPGTAYIIENCEMKPQRYEIYCLAKRNETSYCILADGELSSSKHDTPVFHIKERLPTKEILDCLEFKLNCSVAHKKKVVGPNYLMHLKKIINQVNDEMPEGSRPVLKDCEGKILRMVKLNPLDLDGIEEVYREIVRCELATRGFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASF4</accession>
    <name>I7ASF4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">tRNA-dihydrouridine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_071110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264859.1">
        <property type="nucleotide sequence ID" value="XM_009266584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASF4"/>
    <dbReference type="GeneID" id="20521671"/>
    <dbReference type="KEGG" id="ero:EROM_071110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071110"/>
    <dbReference type="HOGENOM" id="CLU_013299_2_1_1"/>
    <dbReference type="OrthoDB" id="10262250at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004653">
        <property type="entry name" value="DusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42907">
        <property type="entry name" value="FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42907:SF1">
        <property type="entry name" value="FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0820">tRNA-binding</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="DUS-like FMN-binding" evidence="5">
        <location>
            <begin position="108"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="446" mass="50993" checksum="460F52C6B980017D" modified="2012-10-03" version="1">MTFFLGVSTFFGVVVILLILRDLLLFNGLSTSFLTSMSTEFTFLRPFFALHILGFSIEQDPSYYLFVFWREYLYGYCSPYNTDTREIWPENMIGLDTSPMEQRIEISLAPMMGVTTPHYRRFIRLTSETTVLFTEMIVSNTVIHIPRDKLKEKLGEYDDKTVVQIGGSDPVQIIEAVRIIQSLGYKMFNLNCGCPSSRVKKGSFGAVLMLNKELVAEIINRVYKETGEVLSLKIRTGVDDEDGIGFLSGFVGYIRKNTPNRTFYIHARKCWLEGLSPGQNRKLPPLDYDSVYEIKKLYPELKIILNGSISENNLDRVSGLDGAMIGREAIRNAFVFWDIDQQLHGGQGVEMWNGSKETCPLDSSMRRIECNEDHKSKRVQKIHSVVKQYFEGFSPSIQLKPAHILPVMNLVRGKKGCKAYRRKLNSALIHQIRPSEVYPLIMEHFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASK5</accession>
    <name>I7ASK5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MADS domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_071660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264914.1">
        <property type="nucleotide sequence ID" value="XM_009266639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASK5"/>
    <dbReference type="GeneID" id="20521730"/>
    <dbReference type="KEGG" id="ero:EROM_071660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071660"/>
    <dbReference type="HOGENOM" id="CLU_114209_0_0_1"/>
    <dbReference type="OrthoDB" id="2284405at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00266">
        <property type="entry name" value="MADS_SRF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000002">
        <property type="entry name" value="Serum response factor b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050142">
        <property type="entry name" value="MADS-box/MEF2_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033897">
        <property type="entry name" value="SRF-like_MADS-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48019">
        <property type="entry name" value="SERUM RESPONSE FACTOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="7">
        <location>
            <begin position="95"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="171"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="210" mass="24380" checksum="37DE12DD126D9960" modified="2012-10-03" version="1">MDENRKYGKKEEQSNPQIRGEAEYNIPSYGINENIPEGFSYPHWIPQNMNYTFDQQYNYYNHSMPQFHNGGMMPQGFVQEQMHPIMFEKPRGKSHGKKKIKLEYISGKNKRSVCFSKRKRGIMKKAYELNVLTGTEILLLVASESGHVFTFATEKLKPIITEHEDIIQNCLNRPEGTPYKPNFSKGSERQNSENKGYGKAEPLDKSREGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASV3</accession>
    <name>I7ASV3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Coatomer subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Beta-coat protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_081110</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
            <orientation evidence="1 10">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasmic vesicle</location>
            <location evidence="10">COPI-coated vesicle membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265024.1">
        <property type="nucleotide sequence ID" value="XM_009266749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASV3"/>
    <dbReference type="GeneID" id="20521846"/>
    <dbReference type="KEGG" id="ero:EROM_081110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081110"/>
    <dbReference type="HOGENOM" id="CLU_012709_0_0_1"/>
    <dbReference type="OrthoDB" id="10261439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011710">
        <property type="entry name" value="Coatomer_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016460">
        <property type="entry name" value="COPB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029446">
        <property type="entry name" value="COPB1_appendage_platform_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10635:SF0">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07718">
        <property type="entry name" value="Coatamer_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14806">
        <property type="entry name" value="Coatomer_b_Cpla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005727">
        <property type="entry name" value="Coatomer_beta_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5 10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="11">
        <location>
            <begin position="14"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit C-terminal" evidence="12">
        <location>
            <begin position="564"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer beta subunit appendage platform" evidence="13">
        <location>
            <begin position="690"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF14806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="819" mass="93373" checksum="B4A53E144D6C99DD" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT04</accession>
    <name>I7AT04_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Transcription elongation factor SPT4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_081660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the SPT4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265079.1">
        <property type="nucleotide sequence ID" value="XM_009266804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT04"/>
    <dbReference type="GeneID" id="20521901"/>
    <dbReference type="KEGG" id="ero:EROM_081660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081660"/>
    <dbReference type="HOGENOM" id="CLU_138052_2_0_1"/>
    <dbReference type="OrthoDB" id="248751at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07973">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009287">
        <property type="entry name" value="Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038510">
        <property type="entry name" value="Spt4_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882">
        <property type="entry name" value="SUPPRESSOR OF TY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12882:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF025023">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="7">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR025023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="13017" checksum="F5FCA3267BB409D6" modified="2012-10-03" version="1">MEGKFRGTLSGKLRACLGCSQIKTLNGFRKDGCENCPMLGMKGNIANVNECTSSNFKGVVALLQPSNSWIGKWQRIGEFKKGLYAMVIEGVLSEDFIKDIEQHGRVYYERSESFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATF8</accession>
    <name>I7ATF8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoplasmic reticulum junction formation protein lunapark</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in determining ER morphology.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The C4-type zinc finger motif is necessary both for its ER three-way tubular junction localization and formation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lunapark family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265224.1">
        <property type="nucleotide sequence ID" value="XM_009266949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATF8"/>
    <dbReference type="GeneID" id="20564335"/>
    <dbReference type="KEGG" id="ero:EROM_091110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091110"/>
    <dbReference type="HOGENOM" id="CLU_088999_0_0_1"/>
    <dbReference type="OrthoDB" id="1725934at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098826">
        <property type="term" value="C:endoplasmic reticulum tubular network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071788">
        <property type="term" value="P:endoplasmic reticulum tubular network maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903373">
        <property type="term" value="P:positive regulation of endoplasmic reticulum tubular network organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040115">
        <property type="entry name" value="Lnp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019273">
        <property type="entry name" value="Lunapark_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22166:SF12">
        <property type="entry name" value="ENDOPLASMIC RETICULUM JUNCTION FORMATION PROTEIN LUNAPARK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10058">
        <property type="entry name" value="Zn_ribbon_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Lunapark zinc ribbon" evidence="2">
        <location>
            <begin position="149"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="208" mass="24011" checksum="8B64373E3E35E631" modified="2012-10-03" version="1">MGNVLSKNVPLRESLVRLEEEISKKEKAGKKLSASLDGIRTKSLVGSFVAIVLSMMYSYLDGQSIILFGLGSAFGCYVVRCLLVYLYEMRIRRLESGLETLKERQKEQIALLKKEESFEATRKVIDKYESESMRRHYFGSIKQRRRGMMDSITDVVLGDDPSTMYALICKKCNHHNGLVHPNEYDLNEFYCYNCNELNTRPKNRNNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATI7</accession>
    <name>I7ATI7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265269.1">
        <property type="nucleotide sequence ID" value="XM_009266994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATI7"/>
    <dbReference type="GeneID" id="20564380"/>
    <dbReference type="KEGG" id="ero:EROM_091560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091560"/>
    <dbReference type="HOGENOM" id="CLU_1704221_0_0_1"/>
    <dbReference type="OrthoDB" id="2190913at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="153" mass="17213" checksum="8BC2938BB4371B92" modified="2012-10-03" version="1">MDVSSIILKLLSDGIVDGEYDSVLTTLNELLRPIQYEAVRWPDGSCIVLKDNRSSWPPDLGIEEVEDVFRRVVCGMPVSGDIVDMLIQERWIENVEGNPQLSRRSLVQHTDFILGLGGRYVICDVCGFLNEEGGIHGFCKALLEKERGFDEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATJ7</accession>
    <name>I7ATJ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Sulfite reductase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_091710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265284.1">
        <property type="nucleotide sequence ID" value="XM_009267009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATJ7"/>
    <dbReference type="GeneID" id="20564395"/>
    <dbReference type="KEGG" id="ero:EROM_091710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091710"/>
    <dbReference type="HOGENOM" id="CLU_060137_0_0_1"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030586">
        <property type="term" value="F:[methionine synthase] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050667">
        <property type="term" value="P:homocysteine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF84">
        <property type="entry name" value="METHIONINE SYNTHASE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="5">
        <location>
            <begin position="47"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="387" mass="45234" checksum="66F72E3C1F353B9F" modified="2012-10-03" version="1">MALNPIKFEYKEDYTKNIPRIFNKAEVVFNDIGHSPRKFIFPCTENIEYRRVRIVEIEKLEASWRDVYRIKFDEKFHFKPGDSIGMLCPNNDTLVDEIMEVFEVKNSYCQIRRPGKNSFSYTGSIRDFFKYYFDFTSLPRKSLLANLAKGCTETNRKYIEYLCSKEGIVDYLGMGRRWNNIIDIIKTFECRPVLEEVIGECEIVKPRYFSLINKIGEESEILVGITNKIFDGFVRYGHVSDFLVRSEVEEVELSLRINLLFRMDYGSRKLLAICTGTGIAPFLSFASNLQPHQSIWIIYGFRNDEDDLSKLIGPDKNVRVSRVKSSTGIYVTDYLVERIDEIKGYVDEECIAYACGKMETQKRVFEIFKNELPHVVEAKRLVFDQWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATV9</accession>
    <name>I7ATV9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ubiquitin fusion degradation protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_101070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265419.1">
        <property type="nucleotide sequence ID" value="XM_009267144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATV9"/>
    <dbReference type="GeneID" id="20564537"/>
    <dbReference type="KEGG" id="ero:EROM_101070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101070"/>
    <dbReference type="HOGENOM" id="CLU_102810_0_0_1"/>
    <dbReference type="OrthoDB" id="422728at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="3">
        <location>
            <begin position="19"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="4">
        <location>
            <begin position="108"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="227" mass="25851" checksum="386774135927BCB0" modified="2012-10-03" version="1">MLFDLFGLFGEKPSWSLSPIKFDGGNQNNFGGKVIVPQSVLVDLVSFQIQPPFTFEISHSDGIYKTHCGVLEFTGEEGQVVVPSWMYQQLAMEDADEVVLRYMTFPIGKFVKLIPHSVDFLEIENPKLELESCLRNYQVLSEGDEILCQFDEIGSIRFTVAHIEPPSNAVYIVDTDLAVDFLEPIGFKDKMERERTVTKYIEVIDSAHDIKPIRMKKLGLYMYKPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU19</accession>
    <name>I7AU19_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">hydroxymethylglutaryl-CoA reductase (NADPH)</fullName>
            <ecNumber evidence="2">1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_101620</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34" evidence="2"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265474.1">
        <property type="nucleotide sequence ID" value="XM_009267199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU19"/>
    <dbReference type="GeneID" id="20564592"/>
    <dbReference type="KEGG" id="ero:EROM_101620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101620"/>
    <dbReference type="HOGENOM" id="CLU_001734_2_0_1"/>
    <dbReference type="OrthoDB" id="310654at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="entry name" value="3-hydroxy-3-methylglutaryl-coenzyme A Reductase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="entry name" value="Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572:SF24">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL-COENZYME A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="NAD-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="Substrate-binding domain of HMG-CoA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="11"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="413" mass="45371" checksum="A32A84F3D380C21D" modified="2012-10-03" version="1">MLGSKFLHIMLRSLMENINKEANERAKKLRERIEEATGIRIGGVEYEEDYSEVINRCCENLIGYKKVPLGVSSRGVLINNREFFIPIATTEGALVASMNRGIKLMNACGGVEGYAENIGITRGFIMRFKTFGEAMRFYQWIKVEESVANLKRIGNKGSRYLRINKIESKHVVGEDVYIKVYGYSGDAMGMNMITKACVQISSEITRMFPNSSLVSISSNTCTDKKWSGENYCNGRGRRIFMNIKIDDKNCREIIGVGIDKILDVYHSKVVVGGSLVLGGFNCQAANYVAGMFLALGQDLGQVIESSNCVLSMKKFTADVLSVSLFMPSVTVGVIGGGTHLEPSKSFLKQFNKGNDDYIITNKDEDKSSGPGYLGLAVGTAVLGGELSLLGSLANNTLMESHLRLNRGVTQTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU54</accession>
    <name>I7AU54_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">1-phosphatidylinositol 4-kinase</fullName>
            <ecNumber evidence="3">2.7.1.67</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_110300</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.67"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.67" evidence="3"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265509.1">
        <property type="nucleotide sequence ID" value="XM_009267234.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20564629"/>
    <dbReference type="KEGG" id="ero:EROM_110300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110300"/>
    <dbReference type="HOGENOM" id="CLU_253125_0_0_1"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05167">
        <property type="entry name" value="PI4Kc_III_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1010.10:FF:000014">
        <property type="entry name" value="Phosphatidylinositol 4-kinase STT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000016">
        <property type="entry name" value="PIK1p Phosphatidylinositol 4-kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.70">
        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF15">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="1256"/>
            <end position="1278"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="10">
        <location>
            <begin position="889"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="9">
        <location>
            <begin position="1131"/>
            <end position="1408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN84012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1424" mass="165066" checksum="EF492BC44F54A2DB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGQ6</accession>
    <name>I6ZGQ6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA repair protein Rad4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263865.1">
        <property type="nucleotide sequence ID" value="XM_009265590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGQ6"/>
    <dbReference type="GeneID" id="20520650"/>
    <dbReference type="KEGG" id="ero:EROM_010230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010230"/>
    <dbReference type="HOGENOM" id="CLU_052403_0_0_1"/>
    <dbReference type="OrthoDB" id="300780at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-C CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="1">
        <location>
            <begin position="188"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="396" mass="46914" checksum="7D3563204C9A3731" modified="2012-10-03" version="1">MEPSDSWEEAIEDGLELFPRKEKTIRRDVDVKIEMIHRVLWFFRKVVVPVGKPSIKEKVDLHIYERLKEHVSSVGDIGEVDRTVILFNLLISFGIPSRIYIVLSEEPKCFIESKILGKVKEHHSRYEDCVFSIDASLKLKDQSYHFSKSTKRFSASRYVVSGFSKSKMCKDVSDKEMIRCFDEIDNERMSTIPNSVEKMKRHPKYIVESMLRWDQCIYPKRPVFGIFRGEAVYPRENVIRLRTKEQFYKEGKEVRSSKPYRIVKRDKMIRLYAPWQTCEIVVKGFSESMYQDYFHPNFIPQDCVYIDNKNAKDVAYLIGIPYRICFHGFSGRIPINRGIFIEKKNLYVLSNFLSQYCKYLEMKERNERGALGLKRWRVLIRNAAKYLRIRKSLGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGT5</accession>
    <name>I6ZGT5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Thymidine kinase</fullName>
            <ecNumber evidence="2 9">2.7.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_010540</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>thymidine + ATP = dTMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19129"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the thymidine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.21" evidence="2 9"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263895.1">
        <property type="nucleotide sequence ID" value="XM_009265620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGT5"/>
    <dbReference type="GeneID" id="20520680"/>
    <dbReference type="KEGG" id="ero:EROM_010540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010540"/>
    <dbReference type="HOGENOM" id="CLU_064400_2_1_1"/>
    <dbReference type="OrthoDB" id="2194859at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004797">
        <property type="term" value="F:thymidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046104">
        <property type="term" value="P:thymidine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001267">
        <property type="entry name" value="Thymidine_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441">
        <property type="entry name" value="THYMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11441:SF0">
        <property type="entry name" value="THYMIDINE KINASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00265">
        <property type="entry name" value="TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035805">
        <property type="entry name" value="TK_cell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="6 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR035805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="217" mass="24373" checksum="8B1E13263B61CF7E" modified="2012-10-03" version="1">MKRGVLKFVASPMNGGKTTNMLLRAKRASVLGWKVLLAKPQIDTRHSSTIIKSRCGAEMECNLCIDPQFNFMKDISYKDVDILFVDEAQFLSTRQIEELREVVDVHKVSIWCYGLLTDFKKNLFEGSRRLVELCDKMYELDVLCCFCSESGRFHLKYTDGKASLSGPSIEVTTPENDKFVSVCHMCWTEKTSSSEGGCPTLFCGEGVPVDKALEING</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGY8</accession>
    <name>I6ZGY8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_011090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263950.1">
        <property type="nucleotide sequence ID" value="XM_009265675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGY8"/>
    <dbReference type="GeneID" id="20520736"/>
    <dbReference type="KEGG" id="ero:EROM_011090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011090"/>
    <dbReference type="HOGENOM" id="CLU_1245344_0_0_1"/>
    <dbReference type="OrthoDB" id="2195673at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="207" mass="20776" checksum="41DD545B18F81AC4" modified="2012-10-03" version="1">MVRKSLYILAVAGVGIIRAGGLYIPSNVLQDLGKSSNNGCLMIAEVGGNFSVVASGEPVYITEQAGHSEVVWQPVAGENRAVPVYAPTAEEIRESIPSAAGIAPTQYIPPQQGGFVPSSTPVFVAVEEVGPSNGTVVSSTSETCGPKTAGATTGAAAGTTGDKSKAGAAGTTGDKSKNNTSSSKKKKGAKSLMAFGAVVTTALFSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH34</accession>
    <name>I6ZH34_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264000.1">
        <property type="nucleotide sequence ID" value="XM_009265725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH34"/>
    <dbReference type="GeneID" id="20520786"/>
    <dbReference type="KEGG" id="ero:EROM_020280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020280"/>
    <dbReference type="HOGENOM" id="CLU_078876_0_0_1"/>
    <dbReference type="OrthoDB" id="166375at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.200">
        <property type="entry name" value="Plus-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004343">
        <property type="entry name" value="Plus-3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036128">
        <property type="entry name" value="Plus3-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03126">
        <property type="entry name" value="Plus-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159042">
        <property type="entry name" value="Plus3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plus3" evidence="1">
        <location>
            <begin position="84"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="268" mass="31930" checksum="AE2B196C71AF059B" modified="2012-10-03" version="1">MRDGYKLSAHTDFDREELYESEEDKRALLEMPETKREEILYKRHAELQKFKEKKELEEKMMGLKKKAPSVTKEKIYDTDNFMEFSQCIVTRDMIARNVFKPFFDDFVGHFTRARINGLYLVVKIVGVGKGDVYSLWIDRNEIKTNRYLDISTGTRTYKNFRIENISNSPLLREEYEDVWRFFKVGSIAKIREDYEKLAKNMSRRLSDEEISKMVACKDEVYPKRRGITHLKIELIQKRDRAIELKDKKAAMEYQKKIEEIEDGERGGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHQ7</accession>
    <name>I6ZHQ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Subtilisin-like endopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_031060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264225.1">
        <property type="nucleotide sequence ID" value="XM_009265950.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHQ7"/>
    <dbReference type="GeneID" id="20521019"/>
    <dbReference type="KEGG" id="ero:EROM_031060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031060"/>
    <dbReference type="HOGENOM" id="CLU_038703_0_0_1"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707082" evidence="7">
        <location>
            <begin position="18"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="485"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="8">
        <location>
            <begin position="266"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="529" mass="58382" checksum="38270DD6C6BA882F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHZ2</accession>
    <name>I6ZHZ2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_040650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264330.1">
        <property type="nucleotide sequence ID" value="XM_009266055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHZ2"/>
    <dbReference type="GeneID" id="20521129"/>
    <dbReference type="KEGG" id="ero:EROM_040650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040650"/>
    <dbReference type="HOGENOM" id="CLU_073661_2_0_1"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000002">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="17622" checksum="77A658D90B88EB60" modified="2012-10-03" version="1">MGVPMVMANNFWKMACFLLILYIVKIKRVGCWKARKPIEKGEYGMNIIINMDYSMSKGKVISQICHGMSCVMYYLFRNEELLHEWKRNGEAKIVLKASGNEIQEIIRLAKKNGIYYHKVCDAGRTQVPPGANTVLILGPALRSNLSPISGHLKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI13</accession>
    <name>I6ZI13_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ski2 ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_040850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264350.1">
        <property type="nucleotide sequence ID" value="XM_009266075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI13"/>
    <dbReference type="GeneID" id="20521149"/>
    <dbReference type="KEGG" id="ero:EROM_040850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040850"/>
    <dbReference type="HOGENOM" id="CLU_002902_0_1_1"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000083">
        <property type="entry name" value="ATP-dependent RNA helicase DOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016438">
        <property type="entry name" value="SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF7">
        <property type="entry name" value="EXOSOME RNA HELICASE MTR4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005198">
        <property type="entry name" value="Antiviral_helicase_SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="79"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="285"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="933" mass="106875" checksum="DE00B3E91FF67FEF" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIB0</accession>
    <name>I6ZIB0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="6">dUTPase</shortName>
            <ecNumber evidence="6">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="6"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264455.1">
        <property type="nucleotide sequence ID" value="XM_009266180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIB0"/>
    <dbReference type="GeneID" id="20521257"/>
    <dbReference type="KEGG" id="ero:EROM_050250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050250"/>
    <dbReference type="HOGENOM" id="CLU_165612_0_0_1"/>
    <dbReference type="OrthoDB" id="10261072at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="7">
        <location>
            <begin position="24"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="121" mass="13566" checksum="D1916E28ABCCC8C2" modified="2012-10-03" version="1">MLEEEIKMKVLDGAKMPQLTNGAYKLYSSSSGRVPSRSGARIPAGFMMKIPKYYFGKIQSTRIKKLGGVVDSDYRGEICVLVYNDTDEDFVYEKGDEIGEMVICKISMPDIKEDIGNRVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIC4</accession>
    <name>I6ZIC4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264470.1">
        <property type="nucleotide sequence ID" value="XM_009266195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIC4"/>
    <dbReference type="GeneID" id="20521272"/>
    <dbReference type="KEGG" id="ero:EROM_050400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050400"/>
    <dbReference type="HOGENOM" id="CLU_676528_0_0_1"/>
    <dbReference type="OrthoDB" id="2188446at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="407" mass="47447" checksum="B1A2D9D337A36B99" modified="2012-10-03" version="1">MRIVSEYDIKLSRESEPVALYRLPSSEGNLRIGTVRYKHQFKYLEIEMKLPKMRQRSQKMEGKKPSVITYESHGLGLPNGQWYGKIDHTKKVIVLRQIECAYLFNPKFGGLGGEGGDKEVDGYQFRKAETREEKEHRTKNINFQIKKMNLEGFTTMEYKRKEMSQDFNDVPKIAVAEDDTKYVIQVRNSIINAKVVNFSELILLYRDQNVVKTALSRYTSFVQGRYILSNVFYEKSLHGIRDKILELFKEREKVPCKDIYALVGDEEFLIEEICRKDGKYFQLKGFNERTNRCDNDGIEQEIAFLVEKYQPCSIETIQKEMGMDLGIIRQNLPGDVAVLANGMLAICKGDEKRKKIVEILVTKKSWKKTEMLRIAQNEPGKIEDFFDVLCEYCELKGNVWVIKERCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIG4</accession>
    <name>I6ZIG4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NOG1 nucleolar GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050810</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264510.1">
        <property type="nucleotide sequence ID" value="XM_009266235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIG4"/>
    <dbReference type="GeneID" id="20521313"/>
    <dbReference type="KEGG" id="ero:EROM_050810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050810"/>
    <dbReference type="HOGENOM" id="CLU_011784_5_1_1"/>
    <dbReference type="OrthoDB" id="415015at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="7">
        <location>
            <begin position="168"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="480"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="519"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="528" mass="61628" checksum="C5DBE02F06F291D6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ55</accession>
    <name>I6ZJ55_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="12">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_070020</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="12"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264750.1">
        <property type="nucleotide sequence ID" value="XM_009266475.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521560"/>
    <dbReference type="KEGG" id="ero:EROM_070020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070020"/>
    <dbReference type="HOGENOM" id="CLU_000487_3_0_1"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02736">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02583">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035698">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035697">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015700">
        <property type="entry name" value="RPC1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="13">
        <location>
            <begin position="227"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1315" mass="147202" checksum="3BBEE96EB0DDDFA1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ88</accession>
    <name>I6ZJ88_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">cAMP-dependent protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_070470</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.11" evidence="1"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264795.1">
        <property type="nucleotide sequence ID" value="XM_009266520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ88"/>
    <dbReference type="GeneID" id="20521606"/>
    <dbReference type="KEGG" id="ero:EROM_070470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070470"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004691">
        <property type="term" value="F:cAMP-dependent protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05123">
        <property type="entry name" value="STKc_AGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000008">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045270">
        <property type="entry name" value="STKc_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353:SF37">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT PRKX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24353">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="7"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="12">
        <location>
            <begin position="262"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="324" mass="36954" checksum="C6C30DF137F65A09" modified="2012-10-03" version="1">MLKIRDFEFVKVVGEGAFGKVYLVKLKNNPSSMFAMKMLDFDEILKQRLADQLESEISILKRLYGCPFIAKLYSTDFHCGKIGLILEYVGGGELFYWLKKCGRFDEKMTRFYAAEIVSALRFIHGKGILYRDLKPENILITSSGHIKLIDFGFAVYENESIYMISGTPEYMSPEKLRSEDDGRASDYWGLGVIIYEMLCGDPPFYDASADTIYHKILESQVTFPSYVSPIARSLIAGLLDKSRATRLGAKGICEIMGHPFFKGIDWEEVENKRVPPPFVPNPSILMNFLEENTKAKDMKGMETVVLRPYKHIKHFQRDQAAKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJE6</accession>
    <name>I6ZJE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264860.1">
        <property type="nucleotide sequence ID" value="XM_009266585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJE6"/>
    <dbReference type="GeneID" id="20521672"/>
    <dbReference type="KEGG" id="ero:EROM_071120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071120"/>
    <dbReference type="HOGENOM" id="CLU_1845084_0_0_1"/>
    <dbReference type="OrthoDB" id="2189745at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="139" mass="17030" checksum="F1320BD451768F71" modified="2012-10-03" version="1">MESITKETRFNELPQQIKQKLINIYRSSHQQYLSMEMLKPTKFNLLYSQTQDLQTRNLQKNFKLLVESAEKLKRMWNLNKDEADFRYLHENLKKALEELKEEVMLYKKNEENREFLDEITMTYAMLRNKLESLTKEKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJG4</accession>
    <name>I6ZJG4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ubiquitin/L40 ribosomal protein fusion</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_071320</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the 60S subunit of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264880.1">
        <property type="nucleotide sequence ID" value="XM_009266605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJG4"/>
    <dbReference type="GeneID" id="20521692"/>
    <dbReference type="KEGG" id="ero:EROM_071320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071320"/>
    <dbReference type="HOGENOM" id="CLU_1927569_0_0_1"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="14445" checksum="919E6F754F0DB24E" modified="2012-10-03" version="1">MQISVRFGGKTSFIQAEPSQLILSLKTLVGNACSLDSSMMALQHNSKALEDSKTVASYGIRNFDTIVMYPRVLGGGGNMSENDGAMAMKEKNDCLICRRCYARSGKSAQKCRKCDSKDLRPKKPLKTMKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJW0</accession>
    <name>I6ZJW0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP,ATP carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_081320</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ADP/ATP translocase tlc family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265045.1">
        <property type="nucleotide sequence ID" value="XM_009266770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJW0"/>
    <dbReference type="GeneID" id="20521867"/>
    <dbReference type="KEGG" id="ero:EROM_081320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081320"/>
    <dbReference type="HOGENOM" id="CLU_023964_1_0_1"/>
    <dbReference type="OrthoDB" id="2190844at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004667">
        <property type="entry name" value="ADP_ATP_car_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187:SF1">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03219">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="430"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="559" mass="63278" checksum="1DC91866A882BDA3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK09</accession>
    <name>I6ZK09_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_081820</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265095.1">
        <property type="nucleotide sequence ID" value="XM_009266820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK09"/>
    <dbReference type="GeneID" id="20521917"/>
    <dbReference type="KEGG" id="ero:EROM_081820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081820"/>
    <dbReference type="HOGENOM" id="CLU_051220_0_0_1"/>
    <dbReference type="OrthoDB" id="60984at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429:SF0">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE 1-DEHYDROGENASE G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="4"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-6-phosphate dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="156"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="433" mass="49731" checksum="8FCB8777DA5A261F" modified="2012-10-03" version="1">MKVVIFGSSGDLAKRKLFPALSRINLEDVEVVGYARTKFDIEFSEVLEEVGNYSPEFLSRVAYIRGSYDDLSKLRDIVDSETVLYFSVPPSVYTCLFREISKLDYKVVGIEKPYGETKESFEKIKEFDLSRAKFIDHYLLKPLVVVMPGIIRESKALQEVMDNKHVKTVEIISKEVLGGEGRSYFDKNGIIKDIVQGHMAELLGVIASDVTEPSRTMEASERLKVFKECTIDIEKCLYGQYDDYNKEIHRDSSTETFCMIPINISSPRWSKVPFIIVAGKGMNEKRTEIVIEFRRDAFAKCIDLFCGDQPSGRTVHTNEIEAVRLVFNIYPECEVFLEVLVGSDPIRHVLHDKKNINDLMHDSHGEYHDYEIIFDSLIRDKEFSSVSSHEAEVLWNVFDSILSIDKGDVLFYYSKGIDMPKEAEEMIRKIKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK53</accession>
    <name>I6ZK53_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 5B</fullName>
            <ecNumber evidence="3">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Translation initiation factor IF-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_090050</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="3"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265120.1">
        <property type="nucleotide sequence ID" value="XM_009266845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK53"/>
    <dbReference type="GeneID" id="20521944"/>
    <dbReference type="KEGG" id="ero:EROM_090050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090050"/>
    <dbReference type="HOGENOM" id="CLU_002656_3_3_1"/>
    <dbReference type="OrthoDB" id="4928at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03703">
        <property type="entry name" value="aeIF5B_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16266">
        <property type="entry name" value="IF2_aeIF5B_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01887">
        <property type="entry name" value="IF2_eIF5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000013">
        <property type="entry name" value="eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10050:FF:000002">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000112">
        <property type="entry name" value="Eukaryotic translation initiation factor 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="entry name" value="Translation initiation factor IF- 2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003078">
        <property type="entry name" value="PRK04004.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381:SF4">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14578">
        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="Initiation factor IF2/eIF5b, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 15" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="14">
        <location>
            <begin position="72"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="662" mass="73781" checksum="CA72821FD2F4D8A0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK73</accession>
    <name>I6ZK73_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative nucleic acid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_090360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265150.1">
        <property type="nucleotide sequence ID" value="XM_009266875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK73"/>
    <dbReference type="GeneID" id="20521974"/>
    <dbReference type="KEGG" id="ero:EROM_090360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090360"/>
    <dbReference type="HOGENOM" id="CLU_106416_0_0_1"/>
    <dbReference type="OrthoDB" id="446759at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.210.10">
        <property type="entry name" value="Nin one binding (NOB1), Zn-ribbon-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036283">
        <property type="entry name" value="NOB1_Zf-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014881">
        <property type="entry name" value="NOB1_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08772">
        <property type="entry name" value="Zn_ribbon_NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144206">
        <property type="entry name" value="NOB1 zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuclease PIN" evidence="5">
        <location>
            <begin position="4"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Nin one binding (NOB1) Zn-ribbon-like" evidence="4">
        <location>
            <begin position="132"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="225" mass="26360" checksum="0CFC8D083561F358" modified="2012-10-03" version="1">MIAVIDTGYLIERLLPTEGLIRGYVTDSVINELKTAESRAYLEFLSFMIEVRNPSEEYVTKVKNDLRKEVNNLSDTDIDVVALTLELKDEVTEMWLGPESPEQEEVICFTNDNGIKNVLSRYSSYDDPEFSARKYKTRCYGCFSLFSENLDFCKKCGLRTLTRITVADTKNGEMMFFKKGYQYRKPRTLKNTRGVELRSADQREYIQHQKVMRSRMNRNRKEIDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKN7</accession>
    <name>I6ZKN7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Translation initiation factor eIF-2B subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265370.1">
        <property type="nucleotide sequence ID" value="XM_009267095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKN7"/>
    <dbReference type="GeneID" id="20564487"/>
    <dbReference type="KEGG" id="ero:EROM_100570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100570"/>
    <dbReference type="HOGENOM" id="CLU_036210_0_0_1"/>
    <dbReference type="OrthoDB" id="424572at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051956">
        <property type="entry name" value="eIF2B_epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45887:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="554" mass="63818" checksum="71E1A0AFECBD23F1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL33</accession>
    <name>I6ZL33_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vesicle-associated membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_110510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265530.1">
        <property type="nucleotide sequence ID" value="XM_009267255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL33"/>
    <dbReference type="GeneID" id="20564650"/>
    <dbReference type="KEGG" id="ero:EROM_110510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110510"/>
    <dbReference type="HOGENOM" id="CLU_130507_0_0_1"/>
    <dbReference type="OrthoDB" id="264603at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF6">
        <property type="entry name" value="AT11025P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50202">
        <property type="entry name" value="MSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="MSP" evidence="7">
        <location>
            <begin position="3"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="157" mass="18814" checksum="6EBFDE73D1DEACF4" modified="2012-10-03" version="1">MLDIKVMPQTTIRVKRNKRSSTFVVQNNSMESVGYKIKTTKPRDYIVRPNMGLIVPMQQAEVEVTLSESTVPDSSHKFLIEIYRFDWRRSLNDFKQYLRSSLPKPCWTSRIGILYEEEEERINKETVECSEDRGVITFFVHLYILFNVFYLFYRFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLG6</accession>
    <name>I6ZLG6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DnaK-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_111720</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="KEGG" id="ero:EROM_111720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111720"/>
    <dbReference type="HOGENOM" id="CLU_415099_0_0_1"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HSC70CB, ISOFORM G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HYPOXIA UP-REGULATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="612"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="612"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="643"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="657" mass="74989" checksum="7ADB1B0E85F573FB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSE6</accession>
    <name>I6ZSE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">D40 domain-containing putative transcriptional repressor for RNA polymerase II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264028.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSE6"/>
    <dbReference type="GeneID" id="20520816"/>
    <dbReference type="KEGG" id="ero:EROM_020560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020560"/>
    <dbReference type="HOGENOM" id="CLU_715779_0_0_1"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="387" mass="43969" checksum="AF9D8373C1F8EF99" modified="2012-10-03" version="1">MFQPGIEAYLDAIKQQYDTIMAENRSLRQENVRLVEANSQYARKIQYYTSLMNRQKHIPSSDPGSSFIRASKCLKRGSDWSIDGDNLCRVSACGRIVMPGKITNAVISPCGRFVAFGCGYKVFVVMESIIWFLNSSSEKMEKYEERMCEGETQEYRICPMDFTQDSLRLYVADGKGAVRVWNLASKSQERLLKSSDPISLKIIRDLVFTIEWDKTVNVYSLDEKILSLNPGEEFGGPMTVSPDGGFVYAVVNRNKILIIDVKGEMTYMTSTNEERILAMSISNERMIMSAGGYGRNVVLYRIKPEKPSCRCQDVIEQKGTILSLGFIRSHLLVGQPEGVMIWDLEEKKSMRIQINESNVIGISTSKNFFTTVDNNGILRIWRYSPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSF7</accession>
    <name>I6ZSF7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ubiquitin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_020660</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009265763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSF7"/>
    <dbReference type="GeneID" id="20520826"/>
    <dbReference type="KEGG" id="ero:EROM_020660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020660"/>
    <dbReference type="HOGENOM" id="CLU_010412_6_3_1"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000158">
        <property type="entry name" value="Polyubiquitin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="77" mass="8688" checksum="78943C23396EEC9E" modified="2012-10-03" version="1">MQIFVKTLTGKTITLEVEASDSIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSM4</accession>
    <name>I6ZSM4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polyadenylation factor subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_021310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for 3'-end cleavage and polyadenylation of pre-mRNAs. Also involved in chromosome segregation where it has a role in chromosome attachment to the mitotic spindle.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264103.1">
        <property type="nucleotide sequence ID" value="XM_009265828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSM4"/>
    <dbReference type="GeneID" id="20520893"/>
    <dbReference type="KEGG" id="ero:EROM_021310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021310"/>
    <dbReference type="HOGENOM" id="CLU_000288_77_0_1"/>
    <dbReference type="OrthoDB" id="16717at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045245">
        <property type="entry name" value="Pfs2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836:SF0">
        <property type="entry name" value="PRE-MRNA 3' END PROCESSING PROTEIN WDR33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22836">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="176"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="218"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="260"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="346"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="408" mass="46132" checksum="4694E971A6B13CD6" modified="2012-10-03" version="1">MNEDGVNIKELISARFQNPKFVYDGKRMRVLQERKVVDYTSGICIQRVRRRLDPLQVNTSFELSPQILNLKSPELCYNDIPSLFTTKIEHISVNKVKCPVNIVRWTPDGRRLMSGTATGEFTLWNGYGCNFETILQAHESPVRGMAWSPSGSFLISSDNFGIIKYWHPSMNNIQIIQGHAEAVRDLSFSHNDSKFCSASDDSTIKVWDSIEAKEESVLRGHNWDVRAAQWHKYKSLIASAGKDNLVKMWDPRTAEELCTLHHHKNTILALKWMGDENYLLTGGKDQVIKMVDIRTMVDVFTYKNHRKEVTSIGTHPCIPDMFVSGGTEGGIYFWQAYTQVPLEVIEEGHEGTVWSLDYHPVGHILASGSVDQSVRFWVRPRPDENAEVSAGGFDDEENNSDGGAIPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSN4</accession>
    <name>I6ZSN4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcription initiation factor TFIID</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_021410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264113.1">
        <property type="nucleotide sequence ID" value="XM_009265838.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSN4"/>
    <dbReference type="GeneID" id="20520903"/>
    <dbReference type="KEGG" id="ero:EROM_021410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021410"/>
    <dbReference type="HOGENOM" id="CLU_150177_0_0_1"/>
    <dbReference type="OrthoDB" id="2193432at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="101" mass="11742" checksum="3A024E33034BEC80" modified="2012-10-03" version="1">MNIDHHFLCPKKLLRLCNKKIDKEVMRNLQLLTEKFLSDIIHRSALLCKHKGKSVVDSSDIRFVIEKDFDYSFGGREIQGSTALPTSEHLERMAEISRQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZST4</accession>
    <name>I6ZST4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coatomer subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_030170</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Peripheral membrane protein</topology>
            <orientation evidence="1 11">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasmic vesicle</location>
            <location evidence="11">COPI-coated vesicle membrane</location>
            <topology evidence="11">Peripheral membrane protein</topology>
            <orientation evidence="11">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the COPG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264133.1">
        <property type="nucleotide sequence ID" value="XM_009265858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZST4"/>
    <dbReference type="GeneID" id="20520924"/>
    <dbReference type="KEGG" id="ero:EROM_030170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030170"/>
    <dbReference type="HOGENOM" id="CLU_021042_0_0_1"/>
    <dbReference type="OrthoDB" id="1074925at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017106">
        <property type="entry name" value="Coatomer_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013040">
        <property type="entry name" value="Coatomer_gsu_app_Ig-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10261:SF0">
        <property type="entry name" value="COATOMER SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08752">
        <property type="entry name" value="COP-gamma_platf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037093">
        <property type="entry name" value="Coatomer_gamma_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49348">
        <property type="entry name" value="Clathrin adaptor appendage domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 11" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8 11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="12">
        <location>
            <begin position="235"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer gamma subunit appendage Ig-like subdomain" evidence="13">
        <location>
            <begin position="543"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="762" mass="86731" checksum="5E21A3D2C73026EA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT59</accession>
    <name>I6ZT59_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264308.1">
        <property type="nucleotide sequence ID" value="XM_009266033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT59"/>
    <dbReference type="GeneID" id="20521107"/>
    <dbReference type="KEGG" id="ero:EROM_040430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040430"/>
    <dbReference type="HOGENOM" id="CLU_1023425_0_0_1"/>
    <dbReference type="OrthoDB" id="2192375at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="30451" checksum="4EEB63686C84A89A" modified="2012-10-03" version="1">MEGTKLFAEASNIPFRLLQGGMDCKEFVVYNDNVFCLTGRNTLIALSSGEEYEFYGDILSMGIHEHYIYLVFNTSKVIVFDAICREVVVNFREMDGCIKKAMISSSGIYLLGFDGYLYRSTFEDIRYAKDTSVYGTKGESIIQDFWVRDDSIFYVTHYGHICKDSDVILKVFDTVTLIVPHKEYLYIVTEGNLLLKYDVVRSSILFKENIGNSKVIGDGVIGCNGMAIDLYKEIFMKVPKDTRWVRRHDKIIYISTISGIFSGSLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTB9</accession>
    <name>I6ZTB9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ubiquitin fusion degradation protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_041200</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin conjugation factor E4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264383.1">
        <property type="nucleotide sequence ID" value="XM_009266108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTB9"/>
    <dbReference type="GeneID" id="20521183"/>
    <dbReference type="KEGG" id="ero:EROM_041200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041200"/>
    <dbReference type="HOGENOM" id="CLU_018981_0_0_1"/>
    <dbReference type="OrthoDB" id="20295at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019474">
        <property type="entry name" value="Ub_conjug_fac_E4_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045132">
        <property type="entry name" value="UBE4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931:SF2">
        <property type="entry name" value="UBIQUITIN CONJUGATION FACTOR E4 B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13931">
        <property type="entry name" value="UBIQUITINATION FACTOR E4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04564">
        <property type="entry name" value="U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10408">
        <property type="entry name" value="Ufd2P_core"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="10">
        <location>
            <begin position="731"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="537"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="804" mass="93525" checksum="422B5A839457E4A2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTD2</accession>
    <name>I6ZTD2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vps72/YL1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_041400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264403.1">
        <property type="nucleotide sequence ID" value="XM_009266128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTD2"/>
    <dbReference type="GeneID" id="20521203"/>
    <dbReference type="KEGG" id="ero:EROM_041400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041400"/>
    <dbReference type="HOGENOM" id="CLU_071116_4_0_1"/>
    <dbReference type="OrthoDB" id="49520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029525">
        <property type="entry name" value="INO80C/Ies6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013272">
        <property type="entry name" value="Vps72/YL1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31200:SF1">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08265">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00993">
        <property type="entry name" value="YL1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Vps72/YL1 C-terminal" evidence="5">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="106" mass="12598" checksum="E81EB94EB5DC0CAC" modified="2012-10-03" version="1">MKKQKICYKRNTYINKGMKFKTLKQMIPSLIEKDHKYVQISNRISVKPGIRLCDLTGLPAPYTCPNTRLFYYNSSVFKRICDMPSDRIQKLFQLREFGKTLVPFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTH3</accession>
    <name>I6ZTH3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase</fullName>
            <ecNumber evidence="2">6.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_050280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264458.1">
        <property type="nucleotide sequence ID" value="XM_009266183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTH3"/>
    <dbReference type="GeneID" id="20521260"/>
    <dbReference type="KEGG" id="ero:EROM_050280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050280"/>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_1"/>
    <dbReference type="OrthoDB" id="1706066at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000001">
        <property type="entry name" value="Acetyl-coenzyme A synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001208">
        <property type="entry name" value="PRK00174.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24095:SF14">
        <property type="entry name" value="ACETYL-COENZYME A SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acetyl-coenzyme A synthetase N-terminal" evidence="8">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="6">
        <location>
            <begin position="62"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="7">
        <location>
            <begin position="515"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="632" mass="70650" checksum="F97906FFCCE907C5" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTQ0</accession>
    <name>I6ZTQ0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Tyrosyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_051150</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="11"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264543.1">
        <property type="nucleotide sequence ID" value="XM_009266268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTQ0"/>
    <dbReference type="GeneID" id="20521347"/>
    <dbReference type="KEGG" id="ero:EROM_051150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051150"/>
    <dbReference type="HOGENOM" id="CLU_035267_0_1_1"/>
    <dbReference type="OrthoDB" id="197206at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00805">
        <property type="entry name" value="TyrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000040">
        <property type="entry name" value="Tyrosine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="337" mass="38687" checksum="FAD28CC0345AD03F" modified="2012-10-03" version="1">MSVEQKLHLITRNLQEILGLEELKTILSERELNVYWGTAITGKPHIAYLVPLMKVKDFADAGCNVKVLLADIHGFLDNLKAPIEKIKYRCVYYEKLIKASLKMLGVDLNRIQFIKGSEFQRSNEYTMDLYKILSITSEHDAKKAGAQVVKQVENPMVSSLVYPAMQALDEVHLSVDAQFGGVDQRKIFTYARKYLPLLNYKKRIHLMSPMLPGLNSEKMSSSDDLSKIDLMDSKEVILKKIKKCFCEEGNKENGLMKIFSHIIFPIFEAKGEKIRIADRDGKEKVFEKYQEFEDEFSSKSIHPGDLKTNAARLIDGIIRPIREEMEKDLKMVKQAYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUC4</accession>
    <name>I6ZUC4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcription initiation factor TFIIE subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070150</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264763.1">
        <property type="nucleotide sequence ID" value="XM_009266488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUC4"/>
    <dbReference type="GeneID" id="20521573"/>
    <dbReference type="KEGG" id="ero:EROM_070150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070150"/>
    <dbReference type="HOGENOM" id="CLU_056967_0_0_1"/>
    <dbReference type="OrthoDB" id="361102at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="291" mass="34495" checksum="D4D136892F480843" modified="2012-10-03" version="1">MDYVPVMNKLIKKVVRKFYEPHHVVITDILLKKILLYDTELCERMKMLSKEVNRLVIRLKEDKIIKYETKVENKEDNGQILRTVYYINYAEVRDVIKYKIFKMTKNLENSIKMAQTEGYMCMECGKEYSSLDAQSLMENYVFKCEDCKGDLVENKKDRSADCMMYSNLMSELDDIVLLLKETDKFSIPSMDYFQVLQMKKEKEDKQMPAPKEEEASSIAFPTVEEFDVGKGYVTDDGSDRRKDIKIDEYVTVNGAKKLFSDITEEDKEMMNETEYEEYYEIYSRYHEDLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUD4</accession>
    <name>I6ZUD4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 15">Aurora kinase</fullName>
            <ecNumber evidence="1 15">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_070300</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1 15"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264778.1">
        <property type="nucleotide sequence ID" value="XM_009266503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUD4"/>
    <dbReference type="GeneID" id="20521589"/>
    <dbReference type="KEGG" id="ero:EROM_070300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070300"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="OrthoDB" id="377346at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032133">
        <property type="term" value="C:chromosome passenger complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051233">
        <property type="term" value="C:spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045143">
        <property type="term" value="P:homologous chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044779">
        <property type="term" value="P:meiotic spindle checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902115">
        <property type="term" value="P:regulation of organelle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072479">
        <property type="term" value="P:response to mitotic cell cycle spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14007">
        <property type="entry name" value="STKc_Aurora"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000235">
        <property type="entry name" value="Serine/threonine-protein kinase ark1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030616">
        <property type="entry name" value="Aur-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24350">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE IAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="10"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="138"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="cross-link" description="Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)" evidence="12">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR630616-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AFN83281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="272" mass="31771" checksum="B58B90803AB76B94" modified="2012-10-03" version="1">MVEYWTLDRFEIGRLLGRGKFGQVWLAREKEKGFIVALKIIPIKEIQTVETARQIRREVEIHSNLKHPNILRMYGHFHDKDHIYLILEYAGKGEFFKFLSEKGGRFGEKEASLYIRQVILALIYMKECNVIHRDIKPENLLLGSDNQLKIADFGWAVYNADKRRMTFCGTMEYLAPEMVNNDIHDSGIDLWCLGILTYEFLIGKTPFESKNRNMREAYKRINSLKYSIPETISSNASDFISKLLVLNSNDRMELTEALNHPFIVKHYGRSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUE9</accession>
    <name>I6ZUE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264798.1">
        <property type="nucleotide sequence ID" value="XM_009266523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUE9"/>
    <dbReference type="GeneID" id="20521609"/>
    <dbReference type="KEGG" id="ero:EROM_070500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070500"/>
    <dbReference type="HOGENOM" id="CLU_1602700_0_0_1"/>
    <dbReference type="OrthoDB" id="2192700at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="167" mass="19381" checksum="A74F6FFA977B3649" modified="2012-10-03" version="1">MRKVNKAIHSDPNVEELLLSLQIENESLEENSGSEEVSEDSHESLDEVEYRVIGGSRWNSFCEMDFDAKALKDKGEYKVTRGPLPFSLFEFIGDKGNTESTIVTDQKLSIEGGRYDYGFTPSDLKQYRIKRKKKTKKVCPNWEDISEKKRNEPKRICVRSLRLKNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUK2</accession>
    <name>I6ZUK2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein FAM72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM72 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264873.1">
        <property type="nucleotide sequence ID" value="XM_009266598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUK2"/>
    <dbReference type="GeneID" id="20521685"/>
    <dbReference type="KEGG" id="ero:EROM_071250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071250"/>
    <dbReference type="HOGENOM" id="CLU_127817_0_0_1"/>
    <dbReference type="OrthoDB" id="2526683at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026768">
        <property type="entry name" value="YPEH2ZP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31841">
        <property type="entry name" value="PROTEIN FAM72A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31841:SF1">
        <property type="entry name" value="PROTEIN FAM72A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14976">
        <property type="entry name" value="YPEH2ZP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15308" checksum="340F721C6E2A1D4E" modified="2012-10-03" version="1">MTPYKDRKERYVIRCFGCGNVICRRAARSLLLSSLRIKVYSTNVPTENVKCIGNSYMAYSCKCIVADIECRLCRLILGYHVLQLCELCAGEGKNGRTWIFDASSVISTIIKRCSKACLNMFSSYHEEGDSEVKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUM0</accession>
    <name>I6ZUM0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071500</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264898.1">
        <property type="nucleotide sequence ID" value="XM_009266623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUM0"/>
    <dbReference type="GeneID" id="20521714"/>
    <dbReference type="KEGG" id="ero:EROM_071500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071500"/>
    <dbReference type="HOGENOM" id="CLU_696441_0_0_1"/>
    <dbReference type="OrthoDB" id="2191444at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="396" mass="46180" checksum="72A4DC58890FF72E" modified="2012-10-03" version="1">MENILRNEIKKAECCSSSIKDILARCEDAVGFIELYLKRNPGKKHSEFLDFYDDLDVPSQVLLYEKKKKMVRSDKFEEECLMRYCIEQRSEISYEIIKSMRNSKLLFKVLSESFLLVNVDEEDDLFFTKCIVAALRIHNIDFSEMNVLMTGIGRHFSDGRRKYYEHGAIVASVLLNTCEFDIGSMDETQQMIDDIPSHLLKKNGDRDFEDPGNVFKCYKKVENSFEMLGSGWRPKFLQEAINAIEEERDAEKVEASFRWFPDLVRSATERTLKYKSKKALEVLMNYDGYEEHKVDAISSLIQRSHKFLIDDTIDDFFNGKLCLRHKIILVFSFKKIIKEGPLDQAISLYRCIEFMARRIHHEIPRAMGRALECLLVEGMERAGERTDRAVDVLTDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVM8</accession>
    <name>I6ZVM8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265293.1">
        <property type="nucleotide sequence ID" value="XM_009267018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVM8"/>
    <dbReference type="GeneID" id="20564405"/>
    <dbReference type="KEGG" id="ero:EROM_091810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091810"/>
    <dbReference type="HOGENOM" id="CLU_005230_1_1_1"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="357"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="463"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="577" mass="66827" checksum="13885F34A663A8C7" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVS8</accession>
    <name>I6ZVS8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="7">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_100350</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="7"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265348.1">
        <property type="nucleotide sequence ID" value="XM_009267073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVS8"/>
    <dbReference type="GeneID" id="20564465"/>
    <dbReference type="KEGG" id="ero:EROM_100350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100350"/>
    <dbReference type="HOGENOM" id="CLU_056776_7_3_1"/>
    <dbReference type="OrthoDB" id="680339at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051884">
        <property type="entry name" value="Bis(5'-adenosyl)-TPase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="8">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="6">
        <location>
            <begin position="90"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="3">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="5">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="17659" checksum="723002BFD67BAFB9" modified="2012-10-03" version="1">MEFGDKIIPYDHVIVRTKHSFVFTNLRPFLPLHILISPISKKQRIYELTNEETSDLFNTVRVAMKGLKDLCDGFTLGVQDGPCAGQTVFHVHVHIVPRVSGDLKRNDDIYEKGALDSVDRPAREYGEMKKEADKLKEIIGKAFDSEGLYYQKTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVY8</accession>
    <name>I6ZVY8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265423.1">
        <property type="nucleotide sequence ID" value="XM_009267148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVY8"/>
    <dbReference type="GeneID" id="20564541"/>
    <dbReference type="KEGG" id="ero:EROM_101110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101110"/>
    <dbReference type="HOGENOM" id="CLU_1835159_0_0_1"/>
    <dbReference type="OrthoDB" id="2191485at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="140" mass="15945" checksum="02164B2C93784946" modified="2012-10-03" version="1">MCSNIRIIERNNSAQIDVLLYDHVLAHIILDTRMEPSSVRQFSINSLGNNQNSSVKVFVNSRKVDFAYELILGYVLGCVGIEEYDCKVYLFDEIDDLCVLKNLIFAIDEKFNIELKASSEDGRIMIALRGKIVYETTAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWB7</accession>
    <name>I6ZWB7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication complex GINS protein PSF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_110790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GINS3/PSF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265558.1">
        <property type="nucleotide sequence ID" value="XM_009267283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWB7"/>
    <dbReference type="GeneID" id="20564678"/>
    <dbReference type="KEGG" id="ero:EROM_110790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110790"/>
    <dbReference type="HOGENOM" id="CLU_117799_0_0_1"/>
    <dbReference type="OrthoDB" id="10251744at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21693">
        <property type="entry name" value="GINS_B_Psf3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010492">
        <property type="entry name" value="GINS_Psf3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038437">
        <property type="entry name" value="GINS_Psf3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055221">
        <property type="entry name" value="PSF3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22768:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22466">
        <property type="entry name" value="PSF3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160059">
        <property type="entry name" value="PriA/YqbF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF3 N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="163" mass="19287" checksum="58E3E40F81BD6AC7" modified="2012-10-03" version="1">MEYYNAEDILLNESKIPVTFKHKIQNFGFLGVRSKILPENRKVDVPYFLVDFLLRNGHCELDMGVLPESLLSDIKAKPSAVDLRSVCPYFFYLYSTLLREEVSLSEFFYERIRDYSPLVLKKAFSEDDIWRLDMTERNLVVHSRRVFQRFYSFLVVGHSKATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWL1</accession>
    <name>I6ZWL1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Sec20 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265653.1">
        <property type="nucleotide sequence ID" value="XM_009267378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWL1"/>
    <dbReference type="GeneID" id="20564774"/>
    <dbReference type="KEGG" id="ero:EROM_111750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111750"/>
    <dbReference type="HOGENOM" id="CLU_131640_0_0_1"/>
    <dbReference type="OrthoDB" id="2195934at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056173">
        <property type="entry name" value="Sec20_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03908">
        <property type="entry name" value="Sec20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Sec20 C-terminal" evidence="7">
        <location>
            <begin position="78"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="165" mass="19297" checksum="334912978C7202C7" modified="2012-10-03" version="1">MEKKINTELSRTEFSSQEDLRRHASFVQTMIRDYEKNLYEKGEYDDSSKSRVKDWRDRLYKITQEHKDTSEGAENLDELENTVKLVHRQIGKADTNQQILGKSTLKLMGLNYTSNDIEKVLVDTRRKIKESQRQERTEGFLVLAALLVFMLVCILILFDKFVSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACT8</accession>
    <name>I7ACT8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263897.1">
        <property type="nucleotide sequence ID" value="XM_009265622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACT8"/>
    <dbReference type="GeneID" id="20520682"/>
    <dbReference type="KEGG" id="ero:EROM_010560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010560"/>
    <dbReference type="HOGENOM" id="CLU_1320879_0_0_1"/>
    <dbReference type="OrthoDB" id="608866at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="CDD" id="cd11660">
        <property type="entry name" value="SANT_TRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46993">
        <property type="entry name" value="MYB TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46993:SF4">
        <property type="entry name" value="MYB-LIKE HTH TRANSCRIPTIONAL REGULATOR FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="39"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="2">
        <location>
            <begin position="39"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="209" mass="23813" checksum="A1E4C5A658A9A4A0" modified="2012-10-03" version="1">MERTEHTGPSSKLKLHIYSKVPKKKHVRENVVVRSYTKNPRRKPKPWSLEEEEALLRGIKELGHGKWKEILEKYKDVFQEGRRHIDLSDKIRVMNKKASYYYTTKINFIEVNDEGEEVSNALGERRVYPSKFPYSAAKKAGLYRLSSGVKEAIINIQGERNGVIVRHKYLASASPGSRTGINLVKIGSETIERAAAQPKDTGEAFSAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACX3</accession>
    <name>I7ACX3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263937.1">
        <property type="nucleotide sequence ID" value="XM_009265662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACX3"/>
    <dbReference type="GeneID" id="20520723"/>
    <dbReference type="KEGG" id="ero:EROM_010960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010960"/>
    <dbReference type="HOGENOM" id="CLU_737760_0_0_1"/>
    <dbReference type="OrthoDB" id="2189058at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="375" mass="44615" checksum="E5F74B82767BA4BE" modified="2012-10-03" version="1">MHIFRTKEEAANHLRTKFTDAHEVKDLIERHGIAMKRGCYNSYEAKVVDEAIRRFLEERGMEMKNLYGFFLEEEKEFPIRELLIEISNALRQRTMNSIWVYVSYHYHPYIDAKWEPENEIQLLNLVRVLGFKWKEICGIMNKTSRKCISMYYRIMGFNYLYRKSFKMPEEGIPTTDEEWEGLCDRLRTNRKRLSHLINGYISSKLVVPFWNEYNNMALMGYVILHNHFCSVDIKIREILKLIDEGGIEAPDDEIVGREKIKEEISKLIPDLCNYELDIPIDAEDIFWRTIKLFVKFSSGLLRSRFIQITKVYGIRTFKDLIEVFRNLAVDCYLYKIKDKIRDEVSKIMTQKKTRRRSTKDLVTREGTGVVKESCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADT7</accession>
    <name>I7ADT7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_031280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264247.1">
        <property type="nucleotide sequence ID" value="XM_009265972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADT7"/>
    <dbReference type="GeneID" id="20521042"/>
    <dbReference type="KEGG" id="ero:EROM_031280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031280"/>
    <dbReference type="HOGENOM" id="CLU_042314_0_0_1"/>
    <dbReference type="OrthoDB" id="2190529at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="328"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="503" mass="57989" checksum="1025BA6E0F24D3BF" modified="2012-10-03" version="1">MEDLKTFIRLNMRSSCCVEEEGSDQIETFKNGIVSLNLLLGITSTNEIERSRLNYVMNLCLTHPNRFPVSNRLSELEKACMKLSFGEYESIDLSMYGFRHELVGSVPTNSGTYENPILIVRCSGTKNGKEHAYFTERYMFENETVQRLTVEEQSILLVEMIKIFNTEVCHDLRRLKVKAYIDRILFPKNEKSEMVKRTANYYLMALNLDPREQEPFHEEITDLVFECPFVLHNEWEKLLGDAYYSYSAYEKALVYFEKYKCNSKAINCLVNLNRGSEAAERALEEIKSIGVPTNRRSKIRLCSMNIILGTIIGKEAYFDAALDAYYSYEPLRAKGIYFLKAGAVEKAINSLEKALNIVPGNTEIQFLYASALTSAKRFSEAAAVYERLVADDQKNTMFLRNLAMCRIQLEDIKNGLTSLKKASIYDQNMIQAYFLMSIKYNIPKEIKYSLERIDPFEDFEDGVMYLIEKKVLSKDEVKFALARNNRIANIVESLIKKIDSCQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEA1</accession>
    <name>I7AEA1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264472.1">
        <property type="nucleotide sequence ID" value="XM_009266197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEA1"/>
    <dbReference type="GeneID" id="20521274"/>
    <dbReference type="KEGG" id="ero:EROM_050420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050420"/>
    <dbReference type="HOGENOM" id="CLU_1235005_0_0_1"/>
    <dbReference type="OrthoDB" id="2190855at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="224" mass="25459" checksum="8526043A383A0766" modified="2012-10-03" version="1">MCEIGKMNKEIANIIFQESGLGNVVSGEDKPVTGETYFSAVNTIFEILLDDEKYLKAVINFETKEYNSAHPQKESERVPGMESKTLEVMKQVLHSSKRLLFENPDGLFEIKRWNPNNVPVSMENFMDSISREVGKSILGDDCIPGFMILYKTGKYHDITSLIEEIPDSSAILGLKITVPIYARWGTLRNPTTNIATYVLLTIFCIRLKIESYVLHLYSSIWSYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF75</accession>
    <name>I7AF75_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidation resistance protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_070890</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OXR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264837.1">
        <property type="nucleotide sequence ID" value="XM_009266562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF75"/>
    <dbReference type="GeneID" id="20521649"/>
    <dbReference type="KEGG" id="ero:EROM_070890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070890"/>
    <dbReference type="HOGENOM" id="CLU_029204_4_2_1"/>
    <dbReference type="OrthoDB" id="26679at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006571">
        <property type="entry name" value="TLDc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354:SF62">
        <property type="entry name" value="MUSTARD, ISOFORM V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23354">
        <property type="entry name" value="NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07534">
        <property type="entry name" value="TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00584">
        <property type="entry name" value="TLDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51886">
        <property type="entry name" value="TLDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TLDc" evidence="5">
        <location>
            <begin position="35"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="211" mass="24296" checksum="2E29AA7D3C95074C" modified="2012-10-03" version="1">MCAEDRQRCFLDSSLPSLPELRFSMIHNGKEYRNSILSGKVVTGLREALEDRYKYSTTWKLLFSTFEDGFSYRTFLESFEEDGWPFVLACKTREGELLGAFFEDRIRISRAVYGKPSTFLFTTAKNKAVQPEDISTDNELMIFTMSKNKEANIYCCPDFLAFGCSGEKFGLLINKSLLDGETYPIETFGNHPLASKSHFRISYIELWLIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFA9</accession>
    <name>I7AFA9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vacuolar ATP synthase proteolipid</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_071290</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Proton-conducting pore forming of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Proton-conducting pore forming subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1). The decameric c-ring forms the proton-conducting pore, and is composed of eight proteolipid subunits c, one subunit c' and one subunit c''.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264877.1">
        <property type="nucleotide sequence ID" value="XM_009266602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFA9"/>
    <dbReference type="GeneID" id="20521689"/>
    <dbReference type="KEGG" id="ero:EROM_071290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071290"/>
    <dbReference type="HOGENOM" id="CLU_135788_0_0_1"/>
    <dbReference type="OrthoDB" id="1744869at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="11">
        <location>
            <begin position="88"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="154" mass="16239" checksum="65AD3EDD97344A22" modified="2012-10-03" version="1">MEWDEKTLSLMISKLGPGLMISLSAIGGGLGFIAGSEGICKAAENVVNTTYSLVPIIFITAPTMYSVILYFMVYDKKIESLRDGLLVLSACVVNGVSSGVAGYSIGHSAKVACVTRSQQKKFNSIFFLILIFGEVVGLLGLVCAMAISSSIGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFJ1</accession>
    <name>I7AFJ1_ENCRO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264992.1">
        <property type="nucleotide sequence ID" value="XM_009266717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFJ1"/>
    <dbReference type="GeneID" id="20521812"/>
    <dbReference type="KEGG" id="ero:EROM_080760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080760"/>
    <dbReference type="HOGENOM" id="CLU_073630_0_0_1"/>
    <dbReference type="OrthoDB" id="2189509at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031537">
        <property type="entry name" value="DUF5092"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17010">
        <property type="entry name" value="DUF5092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="270" mass="30966" checksum="D7C397C25A4B9DB8" modified="2012-10-03" version="1">MENDVLEMVLKGCSRVVFDSDRGGLPSSSLDTETVEVFTLEKFKDLIVSHIKEGKDFIIAEVTTRDPLTESVFRSYYSAIELNRILFCVEGNNNPLYRVTARNPINNLRITGKVFYYKITWRTLSDVVKSRECACDNEMLIKANYFANDEDLLFDPSIRGYFAKNLIEEGYFVHRLEQMESSMVISEDLLENYRDDKFGLKIILYTNIGTILVIFGMCILLGSKEAFIVAMAPLSMTLLFSILFLILYVLLFEAPDGFSSIFNFRKSRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFL1</accession>
    <name>I7AFL1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265017.1">
        <property type="nucleotide sequence ID" value="XM_009266742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFL1"/>
    <dbReference type="GeneID" id="20521839"/>
    <dbReference type="KEGG" id="ero:EROM_081050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081050"/>
    <dbReference type="HOGENOM" id="CLU_912242_0_0_1"/>
    <dbReference type="OrthoDB" id="2191681at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="305" mass="35451" checksum="5AEDB67A65FE427C" modified="2012-10-03" version="1">MESQAEPERNISYTKLAFFFFLLRTTLIGLALLEGPYFAYRRETRTFKVFVIFLDITNILQFIFLCDIIYRKILFQYLLYIPILAYSLTLTITLVVDAHDSDDIYFELEGKIKRLAISYIYAYMGIILGEFLLLPIIYRRLKSDFMWANFKKVGADPVINDAFKRREQFNAIRRFMVIFLCGEVATFNMGIKDHQDLYFFVAGTQIFVLVISSIFLNEEYINQRRFLLIVSGLCTLIGIAYTTVYIALLSTGIGNIRKGSISEVIFYTITMLIFFIGALRDYQAFGKGLKNYYQGNLGIRKKLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFQ1</accession>
    <name>I7AFQ1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265067.1">
        <property type="nucleotide sequence ID" value="XM_009266792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFQ1"/>
    <dbReference type="GeneID" id="20521889"/>
    <dbReference type="KEGG" id="ero:EROM_081540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081540"/>
    <dbReference type="HOGENOM" id="CLU_1427973_0_0_1"/>
    <dbReference type="OrthoDB" id="2195024at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="190" mass="22004" checksum="0008E203A2290758" modified="2012-10-03" version="1">MSEFEGPEEKKLESFTESDISEKLKDGEACKSVKKRILSMNDENEDNSKQIRTNPHYRKNVGASTIQDTLGEGKRNDVLKQAIRKRDLSYINEFLVRKDRGMLLKTLSYDDKEVLGEMLLEFVDQPVRSEALETMREIVTSIRDVSLFIERLKERAVDFSKLIYLKGKIDYLRFTSGGQEEKEPEMIVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFR6</accession>
    <name>I7AFR6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ARF GTPase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081690</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265082.1">
        <property type="nucleotide sequence ID" value="XM_009266807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFR6"/>
    <dbReference type="GeneID" id="20521904"/>
    <dbReference type="KEGG" id="ero:EROM_081690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081690"/>
    <dbReference type="HOGENOM" id="CLU_109502_0_0_1"/>
    <dbReference type="OrthoDB" id="2195824at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="6">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="234" mass="26984" checksum="9A2254CBF68E9675" modified="2012-10-03" version="1">MSEDEVLDKSLVNRFFKYIRMQSNNKRCADCNNLSPIWVTVTYGFFICTECASKHRELGVRITKVKSTILDTWSLSELRRVYVSGNSNAPKLGKVSDLRTKYTKAEWYSRAVDELSKKSERDEPGTSFIDSLAKGNERVLEPRKIEERRMPKFSDSDAKYVEEKSITNEKQSDELKETKIKIDKPVVIKKNTFPSLRTSQKEAFNVEGDESYVKKLGFGALNIQKNDEDRQPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGS6</accession>
    <name>I7AGS6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Histone deacetylase complex protein Sin3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_110060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265487.1">
        <property type="nucleotide sequence ID" value="XM_009267212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGS6"/>
    <dbReference type="GeneID" id="20564605"/>
    <dbReference type="KEGG" id="ero:EROM_110060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110060"/>
    <dbReference type="HOGENOM" id="CLU_015977_0_0_1"/>
    <dbReference type="OrthoDB" id="10265969at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1160.11">
        <property type="entry name" value="Paired amphipathic helix"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013194">
        <property type="entry name" value="HDAC_interact_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003822">
        <property type="entry name" value="PAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036600">
        <property type="entry name" value="PAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039774">
        <property type="entry name" value="Sin3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031693">
        <property type="entry name" value="Sin3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12346">
        <property type="entry name" value="SIN3B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02671">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08295">
        <property type="entry name" value="Sin3_corepress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16879">
        <property type="entry name" value="Sin3a_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00761">
        <property type="entry name" value="HDAC_interact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47762">
        <property type="entry name" value="PAH2 domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51477">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase interacting" evidence="5">
        <location>
            <begin position="414"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="874" mass="102069" checksum="0274D8DD113C9A2E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGT3</accession>
    <name>I7AGT3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CHY zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_110160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265497.1">
        <property type="nucleotide sequence ID" value="XM_009267222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGT3"/>
    <dbReference type="GeneID" id="20564615"/>
    <dbReference type="KEGG" id="ero:EROM_110160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110160"/>
    <dbReference type="HOGENOM" id="CLU_013368_1_2_1"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017921">
        <property type="entry name" value="Znf_CTCHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037275">
        <property type="entry name" value="Znf_CTCHY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319:SF0">
        <property type="entry name" value="AND RING FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G08900)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21319">
        <property type="entry name" value="RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161245">
        <property type="entry name" value="Zinc hairpin stack"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51270">
        <property type="entry name" value="ZF_CTCHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="6">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CTCHY-type" evidence="7">
        <location>
            <begin position="65"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="129"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="227" mass="25635" checksum="968C9BF26036CC16" modified="2012-10-03" version="1">MSCNHYSNNCLVRFECCSKLYPCRLCHDKVESHKANRYEVSQMVCIGCNLLQRKAQSCSQCLRAVSKYFCSKCNLWDSSDGQIFHCNKCNVCRRGDPKIAFHCDACQTCLVATGSKNHIHVENSTSGNCPICAEDMLGSLEILVLLRCGHSLHERCFNEFIRETYTCPICSKPIGDTSTINKKVEHLLEMEPSYPGQEAKNIVKCNNCNNLSKCKSTWDKCPFCGFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGY1</accession>
    <name>I7AGY1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_110780</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265557.1">
        <property type="nucleotide sequence ID" value="XM_009267282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGY1"/>
    <dbReference type="GeneID" id="20564677"/>
    <dbReference type="KEGG" id="ero:EROM_110780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110780"/>
    <dbReference type="HOGENOM" id="CLU_077727_0_0_1"/>
    <dbReference type="OrthoDB" id="277439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006709">
        <property type="entry name" value="SSU_processome_Utp14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14150:SF12">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 14 HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04615">
        <property type="entry name" value="Utp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="327" mass="38554" checksum="95DCFB2F33CDBF59" modified="2012-10-03" version="1">MPVNKISVADLVKFEDGDKMDLAELEENKDSVYLAIREEVFNAAEQRKKKNKRTRENFYEIVPSTSLEKMMDKILHGEKTKESGAMVFERNFKYEFDKKMRRIKRIKSKAYRRIRRQSRLKSKEAMEENTQEKQGAEQDEVRSRIPDVLLKEVEEVEEVKENLPILSFGGDESREENEQEKLVRLAFKDDMEEQEKVFAKEKEEIMNEEAPRVEEVVLPGWGEWAGPGLEVIKTKNNTIRNIVEGIKYSNRKDFNRSHIIINEKAPEVDKRFLAELPFGYTESEYNEKINASVSRERNTLRIFKRLVRAKTCHTNGKIIEPFHYNPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL79</accession>
    <name>I7AL79_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_010650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263906.1">
        <property type="nucleotide sequence ID" value="XM_009265631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL79"/>
    <dbReference type="GeneID" id="20520692"/>
    <dbReference type="KEGG" id="ero:EROM_010650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010650"/>
    <dbReference type="HOGENOM" id="CLU_091759_0_0_1"/>
    <dbReference type="OrthoDB" id="2190184at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013178">
        <property type="entry name" value="Histone_AcTrfase_Rtt109/CBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08214">
        <property type="entry name" value="HAT_KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01250">
        <property type="entry name" value="KAT11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="265" mass="30416" checksum="1ED3D2911BC7C005" modified="2012-10-03" version="1">MDLEIRKHMENSCGDSSVGVQAFVSGGSYLFLLFNREALLVGMTLQRIGARVYIKNVDTTGCERGYAREFIMSILRVLSPDLSCCFSIPKNEYIFNGSSLNKEKKIRGPRELLEYWIGVFEEIYDNVHVWSNHHENISYPFKHMDEIVYFEDDPKDRLRKHFVGGLEEMFAALLCRTDFTNGSLVYGRSCKHGSGCMVEASIGDVDDMESMLRKLDFSTHSKALESTKKFISRFGLAIEYFPIKRFVKPQEDDQACIILTPIRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALM3</accession>
    <name>I7ALM3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Monoubiquitin/carboxy-extension fusion-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_020990</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264071.1">
        <property type="nucleotide sequence ID" value="XM_009265796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALM3"/>
    <dbReference type="GeneID" id="20520860"/>
    <dbReference type="KEGG" id="ero:EROM_020990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020990"/>
    <dbReference type="HOGENOM" id="CLU_010412_2_0_1"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="152" mass="16883" checksum="13208317C84290C4" modified="2012-10-03" version="1">MQIFVKTLTGKTITLEVGASDTIDSVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGAKKKKKDFSTPKKVKKPHVKRGLSWLDNFTITEDKVKVNNVSCEACGVEVFCRKISSGFKCGKCYTVYGKDIASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALZ9</accession>
    <name>I7ALZ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">6-phosphofructokinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_030580</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264176.1">
        <property type="nucleotide sequence ID" value="XM_009265901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALZ9"/>
    <dbReference type="GeneID" id="20520969"/>
    <dbReference type="KEGG" id="ero:EROM_030580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030580"/>
    <dbReference type="HOGENOM" id="CLU_011053_2_0_1"/>
    <dbReference type="OrthoDB" id="537915at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00182"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005945">
        <property type="term" value="C:6-phosphofructokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003872">
        <property type="term" value="F:6-phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070095">
        <property type="term" value="F:fructose-6-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030388">
        <property type="term" value="P:fructose 1,6-bisphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.460">
        <property type="entry name" value="Phosphofructokinase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022953">
        <property type="entry name" value="ATP_PFK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015912">
        <property type="entry name" value="Phosphofructokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000023">
        <property type="entry name" value="Phosphofructokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035966">
        <property type="entry name" value="PKF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697:SF52">
        <property type="entry name" value="ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13697">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00365">
        <property type="entry name" value="PFK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00476">
        <property type="entry name" value="PHFRCTKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53784">
        <property type="entry name" value="Phosphofructokinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00433">
        <property type="entry name" value="PHOSPHOFRUCTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphofructokinase" evidence="10">
        <location>
            <begin position="103"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphofructokinase" evidence="10">
        <location>
            <begin position="568"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="895" mass="99358" checksum="31631FAD478572CE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM02</accession>
    <name>I7AM02_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">U1 small nuclear ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264181.1">
        <property type="nucleotide sequence ID" value="XM_009265906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM02"/>
    <dbReference type="GeneID" id="20520974"/>
    <dbReference type="KEGG" id="ero:EROM_030630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030630"/>
    <dbReference type="HOGENOM" id="CLU_1563343_0_0_1"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="100"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="173" mass="19608" checksum="DB27537FD59A368A" modified="2012-10-03" version="1">MGEGHSSRTLYIRNLNRKLPIREVKRRLALLVTRFSKILKIKMSNKPRLLGQAFVYLDGEVTNEILSNLNGRFFLGNVISVCPAHSDMCIGKRRAFVPTKTLLVTDIPSLVTRDDIEDIFKSFEGLENIRFIKVKSLAFVDFLSSQKASIAYSHFADGQIRHIHGDMKVMPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM24</accession>
    <name>I7AM24_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidylinositol-4-kinase catalytic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264216.1">
        <property type="nucleotide sequence ID" value="XM_009265941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM24"/>
    <dbReference type="GeneID" id="20521010"/>
    <dbReference type="KEGG" id="ero:EROM_030980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030980"/>
    <dbReference type="HOGENOM" id="CLU_002446_6_1_1"/>
    <dbReference type="OrthoDB" id="10264149at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004430">
        <property type="term" value="F:1-phosphatidylinositol 4-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00893">
        <property type="entry name" value="PI4Kc_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF22">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-KINASE BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="3">
        <location>
            <begin position="434"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="711" mass="82869" checksum="E2E3514DC9193F99" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMA7</accession>
    <name>I7AMA7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RAS-related GTP-binding protein Rab11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_040610</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264326.1">
        <property type="nucleotide sequence ID" value="XM_009266051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMA7"/>
    <dbReference type="GeneID" id="20521125"/>
    <dbReference type="KEGG" id="ero:EROM_040610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040610"/>
    <dbReference type="HOGENOM" id="CLU_041217_23_2_1"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="ras-related protein RABA5a"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
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        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DRAB11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="235" mass="26333" checksum="FC17004C41EC09C1" modified="2012-10-03" version="1">MAECFGCGFRYLDIINPRCLFGMPGVTKGENFDYLFKIVLIGDSAVGKTNLLAKLTRNEFYKDSKATIGVEFGTITFKIGEDIVKAQIWDTAGQERYQAIVQAYYRGTSGAVIVYDITNKDSLKKATSLWLKQLKDFSPSEIPIVLIGNKKDLEDYRAIATEKGKEEAVANELSFFETSALTGENVREAFFELAKTIHKKMKAPKASAGKRRLKSVEFKKENLKEVKPNPKRGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMC4</accession>
    <name>I7AMC4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cohesin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_040810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMC4"/>
    <dbReference type="GeneID" id="20521145"/>
    <dbReference type="KEGG" id="ero:EROM_040810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040810"/>
    <dbReference type="HOGENOM" id="CLU_401714_0_0_1"/>
    <dbReference type="OrthoDB" id="2190075at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039662">
        <property type="entry name" value="Cohesin_Scc3/SA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020839">
        <property type="entry name" value="SCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199:SF0">
        <property type="entry name" value="LD34181P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11199">
        <property type="entry name" value="STROMAL ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21581">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51425">
        <property type="entry name" value="SCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCD" evidence="2">
        <location>
            <begin position="133"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="685" mass="78787" checksum="34A96E1A21FF04A3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMX7</accession>
    <name>I7AMX7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264601.1">
        <property type="nucleotide sequence ID" value="XM_009266326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMX7"/>
    <dbReference type="GeneID" id="20521406"/>
    <dbReference type="KEGG" id="ero:EROM_060090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060090"/>
    <dbReference type="HOGENOM" id="CLU_360157_0_0_1"/>
    <dbReference type="OrthoDB" id="2192931at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="660"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="184"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="598"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="664"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="681"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="779" mass="90899" checksum="D95D73CE0D34E0FB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN48</accession>
    <name>I7AN48_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">N6 adenine specific DNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_060550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264646.1">
        <property type="nucleotide sequence ID" value="XM_009266371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN48"/>
    <dbReference type="GeneID" id="20521452"/>
    <dbReference type="KEGG" id="ero:EROM_060550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060550"/>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_1"/>
    <dbReference type="OrthoDB" id="406152at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="5">
        <location>
            <begin position="10"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="164" mass="18502" checksum="EA0F034EAA36DDDB" modified="2012-10-03" version="1">MDWYDPGEDTYTLMDVMEKEDLEMKIVLDLGTSTGILTECLKKKNTVVSSDLNIKALQNHNGGNLVRANLLDSINQQSVDVVVFNPPYVPDTDDPIIGGGHLGRKVIDRFVSLVNIKVVYLLVIEANRPKEVLRLFDNRGYRTKILKVRKILGETIYIIKGEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANB1</accession>
    <name>I7ANB1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Deoxyhypusine hydroxylase</fullName>
            <shortName evidence="9">DOHH</shortName>
            <ecNumber evidence="9">1.14.99.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine dioxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Deoxyhypusine monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LIA1</name>
        <name evidence="10" type="ordered locus">EROM_061480</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[eIF5A protein]-deoxyhypusine + AH2 + O2 = [eIF5A protein]-hypusine + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:14101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12592"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="ChEBI" id="CHEBI:91175"/>
            <dbReference type="EC" id="1.14.99.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="9">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the deoxyhypusine hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.29" evidence="9"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264736.1">
        <property type="nucleotide sequence ID" value="XM_009266461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANB1"/>
    <dbReference type="GeneID" id="20521545"/>
    <dbReference type="KEGG" id="ero:EROM_061480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061480"/>
    <dbReference type="HOGENOM" id="CLU_053974_0_0_1"/>
    <dbReference type="OrthoDB" id="421002at2759"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019135">
        <property type="term" value="F:deoxyhypusine monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03101">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027517">
        <property type="entry name" value="Deoxyhypusine_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004155">
        <property type="entry name" value="PBS_lyase_HEAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697:SF5">
        <property type="entry name" value="DEOXYHYPUSINE HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12697">
        <property type="entry name" value="PBS LYASE HEAT-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13646">
        <property type="entry name" value="HEAT_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00567">
        <property type="entry name" value="EZ_HEAT"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="285" mass="32002" checksum="535B0DE5B6FC3FA1" modified="2012-10-03" version="1">MDVEIAGNNLKNDGIPIARRMRSLFYLRNILAPESVNAIVGAFGSKSILLKHEAAYVLGQMCMEESVHVLLSILSDENEDEIVRHEAGEALGNFKPTKEIVEGLKKYANHPLKPISETCYLALMKLRNGVNDVSKFGSRDPAPPMEGDFEEARRILLDSSECLYRRYQAMFYLRDLETSDAIHALGKAMEDESSLFKHEISFVFGQMRSIESIPYLIKGMEDVKEHGMVRHECAEALGAIGNDKSLEALVKYLYDPCDILRESVEVAGGIHNYMTGCEIEYCNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANC1</accession>
    <name>I7ANC1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NGG1-interacting factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264751.1">
        <property type="nucleotide sequence ID" value="XM_009266476.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANC1"/>
    <dbReference type="GeneID" id="20521561"/>
    <dbReference type="KEGG" id="ero:EROM_070030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070030"/>
    <dbReference type="HOGENOM" id="CLU_037423_0_1_1"/>
    <dbReference type="OrthoDB" id="3345469at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF13">
        <property type="entry name" value="NIF3-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="242" mass="26914" checksum="236002A3CE090719" modified="2012-10-03" version="1">MSIQEVGKAIGRFASLRRAKTDWDNVGVIVDSGTQDNKVLLTIDLTEPVLEECIELGIKNIVAYHPIIFKAIKKLGSKESIVIECIKNGINVFTPHTALDPLMNNYVYSLVNSGAFLYKKNCGPNFSTIRNAITILKEKSGIKNFRICLARGHTMESIPESMHVGVGATFRNVPLKDSIVVTGEMHHHDLLLCIANNASVILMEHSNSERICLKHISSKLQEELPGYEIFISTRDKDPVMII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANG2</accession>
    <name>I7ANG2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070580</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264806.1">
        <property type="nucleotide sequence ID" value="XM_009266531.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANG2"/>
    <dbReference type="GeneID" id="20521617"/>
    <dbReference type="KEGG" id="ero:EROM_070580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070580"/>
    <dbReference type="HOGENOM" id="CLU_678091_0_0_1"/>
    <dbReference type="OrthoDB" id="5572108at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17003">
        <property type="entry name" value="Actin_micro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="376" mass="43145" checksum="AAB103AB70B569CC" modified="2012-10-03" version="1">MPLKYTPKLCTVPKIDQITTPNKNIQSFFMRSDASYYADEKHRMANYRTDEQIIHILVWNNKLLVESESIDLGDFFYLTGMNTKRFGSLEECVGWLGNQISDVLRKLKIKPGSSFVSLVFQDLLNRTEVLALCNLFINVLSFKGILITPRSLSQAIGTLSPNCVVVNLYEQYTTICFVEDFWMLDGICVSRECNKGFELIDSVDFVDEFNKIKVFEEKNIFSCDKCDYKDETEAKMEAHFLSLHQEESVCHTDVGQSEHFKMHFRVYDGEEGDIHELIAKRTIHVLSKEKAKKIGSKVIVFNYGGMGLSEECLGRAFEEYGINPEISMHEEPNESMITNVMVAFSGLDCVKEIWMTDKEWNGAGLRVLKEKVLFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP50</accession>
    <name>I7AP50_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265081.1">
        <property type="nucleotide sequence ID" value="XM_009266806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP50"/>
    <dbReference type="GeneID" id="20521903"/>
    <dbReference type="KEGG" id="ero:EROM_081680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081680"/>
    <dbReference type="HOGENOM" id="CLU_1366237_0_0_1"/>
    <dbReference type="OrthoDB" id="286814at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="203" mass="23745" checksum="83E60EFA8346F2C1" modified="2012-10-03" version="1">MSTGSGEYGLINDVTLSRFREFLGTSINDGYTRHRICKSLKRSRTSLYTILTAKILFDKTKRYFPSYIEKIEKGSLSDVDKFMSNRRKKELEKILGNEYLLFIGCTIISSKIYMDLSYTNFSWIEVCHHGVDQINVAERQILQVLDYDVNLQWIELKRMYDEFGAVPNKALFGQVEMKQRKGFFKWAFGSLFRFISCVDTVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP94</accession>
    <name>I7AP94_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Protein transport protein Sec61 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265126.1">
        <property type="nucleotide sequence ID" value="XM_009266851.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP94"/>
    <dbReference type="GeneID" id="20521950"/>
    <dbReference type="KEGG" id="ero:EROM_090110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090110"/>
    <dbReference type="HOGENOM" id="CLU_031763_2_1_1"/>
    <dbReference type="OrthoDB" id="420669at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019561">
        <property type="entry name" value="Translocon_Sec61/SecY_plug_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10559">
        <property type="entry name" value="Plug_translocon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="359"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="414"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Translocon Sec61/SecY plug" evidence="3">
        <location>
            <begin position="40"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="474" mass="52954" checksum="23411F62F96FD330" modified="2012-10-03" version="1">MSFNLLHVVKPLVPYIPDVQSPSRTITFHERFIWTTLAILIYLVSSQVPLFGIISNDAADPLQWMRAMMASNRGTLMDLGTSPVVTSSLIMQFLTMSEILKVDWSIKEDKNLHNATQRLISLIMTVGQAFVQVYTGFYGSPKSLGTIYCLLLVMQLIFSGVIIILLDELLQKGYGLGNGVNLFIATNVCESIVWKAFSPKVLFTGRGIEFEGSVIALFHLLVVRKNKFAAIYEAFFRQNLPNLFSLLSTVLLFTLVIYLQGIRVELPTESSQVRGHVAKFPIKLLYTSTMPIIAQNYIVGHISSISSFLYRRWPQNLVVKILGVWNTSKGGRYMPVSGISYYITSPESILEGLRDPLRFFAYLSIMLLTSAILSTSWLELNELSPEKAAQKLKESRMRLKGVREASTATVLSRYIPTAAFLGGILTSMVVILSNLFDAIGSGTNIFLATSIVNQYLDMFAKETTQKNSEIGFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7APH1</accession>
    <name>I7APH1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265231.1">
        <property type="nucleotide sequence ID" value="XM_009266956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APH1"/>
    <dbReference type="GeneID" id="20564342"/>
    <dbReference type="KEGG" id="ero:EROM_091180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091180"/>
    <dbReference type="HOGENOM" id="CLU_980368_0_0_1"/>
    <dbReference type="OrthoDB" id="2195441at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="287" mass="33691" checksum="3CBF3D1B0592EE08" modified="2012-10-03" version="1">MALTYKERLEFLESLKKTPIDLAVADRMVLYARDRALTRPTLLSLVKELTNLDAYISVMHGILTQEEWEEVISDYDTPIEGSHANLREKIKMFLFAYENLSDAIHDFKIDEVLKAFEVSLLSKTRNIQFLLFRLCCRNPQAVFGFLFKLTYKNPTVYLPYLSSLIVRCRIDEGVKSGYVRDYLSYIRSLSRSSSILYVSACQCLLYISCFRREIFMAAKDVIDQIFDSGIARYMNRNVVEMFCDLFGYECKMFSSYDHDCLYFFPFDLPILEKVGDGIHEFYIHFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7APK1</accession>
    <name>I7APK1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Kinetochore Nuf2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_091680</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NUF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265281.1">
        <property type="nucleotide sequence ID" value="XM_009267006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APK1"/>
    <dbReference type="GeneID" id="20564392"/>
    <dbReference type="KEGG" id="ero:EROM_091680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091680"/>
    <dbReference type="HOGENOM" id="CLU_025461_1_1_1"/>
    <dbReference type="OrthoDB" id="8194677at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051383">
        <property type="term" value="P:kinetochore organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045132">
        <property type="term" value="P:meiotic chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.60">
        <property type="entry name" value="Ncd80 complex, Nuf2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005549">
        <property type="entry name" value="Kinetochore_Nuf2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038275">
        <property type="entry name" value="Nuf2_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21650">
        <property type="entry name" value="MEMBRALIN/KINETOCHORE PROTEIN NUF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03800">
        <property type="entry name" value="Nuf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="11" id="KW-0137">Centromere</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Nuf2 N-terminal" evidence="13">
        <location>
            <begin position="7"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="142"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="292"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="436" mass="51050" checksum="BB4810F745C1ECED" modified="2012-10-03" version="1">MQKRNVYAVPDLPIKEIIQYFSEMEINIKASDILKPTPQSTQRIYEILLEVYCGIRTSDMVSRIGNIEAFEESLSYLLLQKRMSGFLRRIGIENFGLRDLAPDSKRLIGILSVVVNFSMFRDNKRHVYERVCRMNDEKLLLRNEIDEKVHNARKELEMCERDAKKSIEESKEVEKEILVLESELKDFYKHQRALVQETERVKAERNEYSDKLSSLRLMLLNLSQEITCLKTQVVSDPTKLMELVDEMRCLISKENEIVRGLEVKRMNLKERIDLMELMKEDTMKAITLSLSNKEADKTIDRTNREISELEVQIKNLDSGINALKIRLNHVNRQISHIESKIFNLQDNDKRCSEEISAKLEKLKNNYGVVSDERNSIRRKIEENVRLTKNIEYELVKKRNEHVNDITAIQSALCRLKDDVFNYFAEAKGVIDKAMPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7APM2</accession>
    <name>I7APM2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxycytidylate deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_092000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265311.1">
        <property type="nucleotide sequence ID" value="XM_009267036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APM2"/>
    <dbReference type="GeneID" id="20564424"/>
    <dbReference type="KEGG" id="ero:EROM_092000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_092000"/>
    <dbReference type="HOGENOM" id="CLU_025810_8_2_1"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="154" mass="17321" checksum="2F227252AFEE9816" modified="2012-10-03" version="1">MGLVITAYSMPHSFFMDIAFAEAAKAFDRLEVPVGCVVVKNGTIVSMSHNMTNANKSPLEHAEIISVRNVDCSNSIFYVTCEPCIMCMGILGRLENVEVYYGCKNEIFGSETICGIGIKSVYLPDNRCFEILRKFYARENTFAPDEKRKSKRHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7APP4</accession>
    <name>I7APP4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Exocyst complex component EXOC2/Sec5 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265341.1">
        <property type="nucleotide sequence ID" value="XM_009267066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APP4"/>
    <dbReference type="GeneID" id="20564457"/>
    <dbReference type="KEGG" id="ero:EROM_100270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100270"/>
    <dbReference type="HOGENOM" id="CLU_529991_0_0_1"/>
    <dbReference type="OrthoDB" id="26242at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039481">
        <property type="entry name" value="EXOC2/Sec5_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15469">
        <property type="entry name" value="Sec5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component EXOC2/Sec5 N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="514" mass="60888" checksum="5C07BC5E4417FA91" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I7APU8</accession>
    <name>I7APU8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Kinesin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_100880</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265401.1">
        <property type="nucleotide sequence ID" value="XM_009267126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APU8"/>
    <dbReference type="GeneID" id="20564518"/>
    <dbReference type="KEGG" id="ero:EROM_100880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100880"/>
    <dbReference type="HOGENOM" id="CLU_001485_2_1_1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF75">
        <property type="entry name" value="CENTROMERE-ASSOCIATED PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="4">
        <location>
            <begin position="9"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="83"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="459" mass="53102" checksum="2EA753557D0C758B" modified="2012-10-03" version="1">MIMHPTHKKIKVSIRLRPSMGDKEVWKTSEDGLWHVHGQKRVKYNGFRSILTNMTNAEVYKECVREEIQKFLNEENCTVFAYGQTGSGKTYTMLGGDEEGIIKLAIRDILDRRPVGISYLEIYNEKLFDLSNNSEVQMFNVENRNVISNLWVEHVDKWEDVASFIERCEKNRKFGTTEFNTKSSRSHTVVQVTYNSNNRVRTLNMIDLAGSERASRSMDRRKEGSFINRSLLALCTIVNNVGNGKYLGFRNSKLTRVLQSSLDGRTNLVAICTLSPYEDCIEESVSTLKFAARLCSLELKIEETLIPNDENMRCDRDMGGNGLRCGLKHKCCRRFHSEAGKEARSILERMESMTLEKCLGSYEYPKIRSKEKKVDYDKVCSEKDSISRALRAFGSVIMENLRCKIELMEELNAINNDRIHLLERMVGELLGKNPSRRMNEIFILEKYRFNLRRKMVGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ00</accession>
    <name>I7AQ00_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Serine/threonine-protein kinase</fullName>
            <ecNumber evidence="10">2.7.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_010420</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="10"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263884.1">
        <property type="nucleotide sequence ID" value="XM_009265609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ00"/>
    <dbReference type="GeneID" id="20520669"/>
    <dbReference type="KEGG" id="ero:EROM_010420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010420"/>
    <dbReference type="HOGENOM" id="CLU_000288_46_1_1"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14099">
        <property type="entry name" value="STKc_PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000042">
        <property type="entry name" value="Aurora kinase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF0">
        <property type="entry name" value="CELL CYCLE SERINE_THREONINE-PROTEIN KINASE CDC5_MSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="24"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="12">
        <location>
            <begin position="307"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="472" mass="54403" checksum="D4C1A9C35FD0C0FC" modified="2012-10-03" version="1">MSTKVELYMEIGTVITDHEYHTRYILKKLLGRGAYAQCYLAEIENGEQYAMKVVRLKDIKSRKVHEKLESEISIHSKLDNPNIVKMYRSFRNEEYVFMVLELCERGALDALLKRNGKLKERHVARFVKQTVEGLMYLHDSVNVVHRDLKLGNLFLDSKFNVKIGDFGLSAVIKDGEKKITMCGTPNYIAPEVLFGKASGHSFEADIWSLGVIIYTLLVGVPPFQKKNVEDIYKMIKLNNYIFPENCDLSSEAIDLITQILNTNPLERPTLEHILGHKFLSRKEHFLMRIYRNLMINKTQEGVIDTDYVLFSIPVTKLRGIGYVLKSGVYGIYFSDHRNLMLKPNKKSVIYLSSAMESGKRVFYKEEHLVEKIPGEILESYKGLQYFIRTFDNGFSFLDVEPCFIVKIRKIDCGFLFVMADSTIVFDFVDGWRVVLSRCGERVSCYNGLGLASFNQEIRMKCIRILKNCLGCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ30</accession>
    <name>I7AQ30_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263914.1">
        <property type="nucleotide sequence ID" value="XM_009265639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ30"/>
    <dbReference type="GeneID" id="20520700"/>
    <dbReference type="KEGG" id="ero:EROM_010730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010730"/>
    <dbReference type="HOGENOM" id="CLU_030988_12_2_1"/>
    <dbReference type="OrthoDB" id="6600758at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="2"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="159" mass="18651" checksum="0A4553A57E225DC1" modified="2012-10-03" version="1">MPNLERLLAEQENLAKNRKYKFYAIPLPEKPKRNITRWECGIPGPPDGVYKGAYYTLYVDFPDDYPFNPPQVMFKYPVYHPNVYPTNQVCLDIIGDRWKPSLNVMNVLCGVQQLLEAPNTRSPANTDASLCFKKDLERYLENVKENIGRYHRRPEWRGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ31</accession>
    <name>I7AQ31_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glycine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Diadenosine tetraphosphate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_101690</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="2"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265481.1">
        <property type="nucleotide sequence ID" value="XM_009267206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ31"/>
    <dbReference type="GeneID" id="20564599"/>
    <dbReference type="KEGG" id="ero:EROM_101690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101690"/>
    <dbReference type="HOGENOM" id="CLU_015515_1_0_1"/>
    <dbReference type="OrthoDB" id="57698at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070150">
        <property type="term" value="P:mitochondrial glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GlyRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000093">
        <property type="entry name" value="Glycyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
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        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
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        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
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        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003211">
        <property type="entry name" value="PRK04173.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745:SF0">
        <property type="entry name" value="GLYCINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="GLYCYL-TRNA SYNTHETASE/DNA POLYMERASE SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="9">
        <location>
            <begin position="5"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="579" mass="66937" checksum="0F3278A6D2E7C2E3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ54</accession>
    <name>I7AQ54_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DASH complex subunit ASK1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265506.1">
        <property type="nucleotide sequence ID" value="XM_009267231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ54"/>
    <dbReference type="GeneID" id="20564626"/>
    <dbReference type="KEGG" id="ero:EROM_110270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110270"/>
    <dbReference type="HOGENOM" id="CLU_105931_0_0_1"/>
    <dbReference type="OrthoDB" id="2192696at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26199" checksum="92CA04D7773DD1C9" modified="2012-10-03" version="1">MIEIEEEIIHTIEEIDTTFSSINRTLKSIREFIERIGRDRRKMDKDLGPWVRFFSVESEDSIGNEAPRSGTEKRLEEEPSFQDICVTEGSPESMKFSSPRNPFVDSTSSEMLNKTFLGDLSARLHSSNVENESSSFVRVSEGKAFRSYEESESSEDILPFNPSMLPPLFKNEKSLFQVYEVISKSSSITIEEIYREISAVPKEKINIFVDLLLRKRFIGKEGDKFRTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ83</accession>
    <name>I7AQ83_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dihydrofolate reductase</fullName>
            <ecNumber evidence="2">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_011280</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263969.1">
        <property type="nucleotide sequence ID" value="XM_009265694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ83"/>
    <dbReference type="GeneID" id="20520755"/>
    <dbReference type="KEGG" id="ero:EROM_011280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011280"/>
    <dbReference type="HOGENOM" id="CLU_043966_5_0_1"/>
    <dbReference type="OrthoDB" id="2190285at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046452">
        <property type="term" value="P:dihydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069:SF3">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00070">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="7">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="205" mass="23425" checksum="9751F4E79527E33A" modified="2012-10-03" version="1">MFALIVALARNKGIGRANRLPWSRPLRTDMSWFRTLSQSIPLITSHHIDLLPSPSNAVVMGRKTWESIPPKFRPLPNRMNIVLSRRPGAKTENAFFIPTFEDLSHLDIPPSSMIFVIGGHDIYNLAIQNGKAQIMFVTEVFESPDCDVFFPHVDWESYEKRDITYEVSRLIDQNLADAFYNTEANAFTENGTTFKMFIYIKPGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQD7</accession>
    <name>I7AQD7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264024.1">
        <property type="nucleotide sequence ID" value="XM_009265749.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQD7"/>
    <dbReference type="GeneID" id="20520812"/>
    <dbReference type="KEGG" id="ero:EROM_020520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020520"/>
    <dbReference type="HOGENOM" id="CLU_123985_0_0_1"/>
    <dbReference type="OrthoDB" id="2188462at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="188" mass="21471" checksum="456990BFF0D8D1CF" modified="2012-10-03" version="1">MDKFHSLAIKALLTFICTTAVLVHEGSFRLYLCVALLLATSFDVSVIFYFHMKRVEYKKRFLRKVYDPGHRTLIGFALCEVLSMVPFYYMVGYWKTMVINIALVASLSIIPMLNSSGRRTIQLERNEEFFKIGFLKIEHIEDGDVCLNKGEVVQILDHTGGSTIVRKSDGRVFTVEDSYIDDGIDIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQG8</accession>
    <name>I7AQG8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264054.1">
        <property type="nucleotide sequence ID" value="XM_009265779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQG8"/>
    <dbReference type="GeneID" id="20520843"/>
    <dbReference type="KEGG" id="ero:EROM_020820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020820"/>
    <dbReference type="HOGENOM" id="CLU_2183921_0_0_1"/>
    <dbReference type="OrthoDB" id="2188950at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="109" mass="13171" checksum="F2E1F782008CC424" modified="2012-10-03" version="1">MKKDYIKDLSTKYLICDHERIRREYLKYKAKNIDTLEYIDDTMLMMYEHAFFYNFTKIKFSRKDLPKEVSPRIFRTALEEVKNKYAPEKNPEDKGLVCNLYSITNPLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQJ2</accession>
    <name>I7AQJ2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glutamate--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_021120</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264084.1">
        <property type="nucleotide sequence ID" value="XM_009265809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQJ2"/>
    <dbReference type="GeneID" id="20520874"/>
    <dbReference type="KEGG" id="ero:EROM_021120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021120"/>
    <dbReference type="HOGENOM" id="CLU_001882_1_2_1"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000037">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12 16" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="13">
        <location>
            <begin position="149"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="14">
        <location>
            <begin position="454"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="15">
        <location>
            <begin position="555"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN82587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="642" mass="74137" checksum="2E9562FD2020ACB8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQS2</accession>
    <name>I7AQS2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264144.1">
        <property type="nucleotide sequence ID" value="XM_009265869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQS2"/>
    <dbReference type="GeneID" id="20520937"/>
    <dbReference type="KEGG" id="ero:EROM_030270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030270"/>
    <dbReference type="HOGENOM" id="CLU_1069692_0_0_1"/>
    <dbReference type="OrthoDB" id="2191548at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="260" mass="30262" checksum="024CCF5902070EAE" modified="2012-10-03" version="1">MRIILTFLFIRKASCYFIFELIDDFVIYYENMFFPNSAGAWAILLGSFFVTFYGSRFPRISLTPYIAALIYYNFCDIREGLNSVSTKTVQEYYIPPDIIRRMKEVFGGSNNDPATLCFISFVLASILMWALEVASSLLFIIGLYIVYRMFFHLGFETYKESNPGVFYILLFVSFGILYYVTRRTTKYLLLILFSITGSLILLTSIELITENNELGFYDLILDFEDTQYLELSSASTPIAVWIITAFIGMFWQWKFSENKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQU7</accession>
    <name>I7AQU7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_030560</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16 17">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264174.1">
        <property type="nucleotide sequence ID" value="XM_009265899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQU7"/>
    <dbReference type="GeneID" id="20520967"/>
    <dbReference type="KEGG" id="ero:EROM_030560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030560"/>
    <dbReference type="HOGENOM" id="CLU_009884_3_0_1"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF687">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="12">
        <location>
            <begin position="108"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="11">
        <location>
            <begin position="249"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="576" mass="66541" checksum="59D52B060E52F506" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR05</accession>
    <name>I7AR05_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RAS-related cell division protein 42-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_031450</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264264.1">
        <property type="nucleotide sequence ID" value="XM_009265989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR05"/>
    <dbReference type="GeneID" id="20521059"/>
    <dbReference type="KEGG" id="ero:EROM_031450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031450"/>
    <dbReference type="HOGENOM" id="CLU_041217_21_3_1"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="179" mass="20259" checksum="1F58623D091925C3" modified="2012-10-03" version="1">METIKCVLVGDPSVGKSWLISTYITGKNPEGYIPTLFNNHSKVVNVGDKKYNMTLWDTAGSEEYNKLRSLSFQETDVFLICYAVNNILSFHNSKRWIDELAEYNVPMILCGTKCDIIDGNIVDKELPTKLSKEYGLYGFVECSSVELINVKKVFDVALEAALKPKKSKFRSIWFCCKCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR77</accession>
    <name>I7AR77_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264359.1">
        <property type="nucleotide sequence ID" value="XM_009266084.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR77"/>
    <dbReference type="GeneID" id="20521159"/>
    <dbReference type="KEGG" id="ero:EROM_040950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040950"/>
    <dbReference type="HOGENOM" id="CLU_1219684_0_0_1"/>
    <dbReference type="OrthoDB" id="2192878at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="213" mass="24605" checksum="3DDB377CA50E4C53" modified="2012-10-03" version="1">MTAEGGNDEMIEESSITVSSGIADESVAFPRDGSPKNQKKSRSCFPKRLWIKRDVDYDLDLSYCDKILNRFIFHVNTLKEGGTYERDINEDAFYYKYMPISPCDIETIKDSPDLRDKMSCRRLREMSLQLIDLCMKLNRDGEGLIDLETTTSELSEDTRALIRDSILKKKRILNLLHKVRNYIGEIILMGEKCYNHIEKSSAIAFKQILSLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARE8</accession>
    <name>I7ARE8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SWIM-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264444.1">
        <property type="nucleotide sequence ID" value="XM_009266169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARE8"/>
    <dbReference type="GeneID" id="20521246"/>
    <dbReference type="KEGG" id="ero:EROM_050140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050140"/>
    <dbReference type="HOGENOM" id="CLU_043427_0_0_1"/>
    <dbReference type="OrthoDB" id="2187956at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31973">
        <property type="entry name" value="POLYPROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="399"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="490" mass="56496" checksum="38948AF8F7A120C9" modified="2012-10-03" version="1">MKGLSVGDVFPTKEEAEKVLISHAVQSNLNYDLEETPKSVAVLCKGRKEFGCEARIVAQVRKKDGVFVVKRVRLSHKCPSVTSKYGSPEEMVRMEVYRAIGDRHARIGEVVSILAGMNIKMGYFSVWKAMRQGNEMEKENIRISPLEENRKTELFETALKEFSHEFLELNPSAIAHHREKMFFFSFPEYIDTLVPIVEIRIYKRIEGFTVLGILRDPTWSPIIYSCVVSDLGSQEDAFRYFVDSMPELFFLVDFDVNLINILKEKKREFFVKTRDICKFYYNKTKSIDTVENIWSKCNNDKEWSIPELNFIDKKQYLKSCCETEMFGLQNTSECDVEFIGLGIFNLPFFDCICGILKLASDNLKQRKKSPLGEGKHLFGNNVIRRIEKNLSMDVTETSHDVDLENLTCTCGRFQEFLIPCSHACKKILDSEEDPYSYVSDLYSKARLLKLKDITPVVDLPVKCQSDRNLLKRGPGRPKKILVREPPIVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARW2</accession>
    <name>I7ARW2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Replication factor A protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264619.1">
        <property type="nucleotide sequence ID" value="XM_009266344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARW2"/>
    <dbReference type="GeneID" id="20521424"/>
    <dbReference type="KEGG" id="ero:EROM_060270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060270"/>
    <dbReference type="HOGENOM" id="CLU_085472_0_0_1"/>
    <dbReference type="OrthoDB" id="25571at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040260">
        <property type="entry name" value="RFA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014646">
        <property type="entry name" value="Rfa2/RPA32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989">
        <property type="entry name" value="REPLICATION PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13989:SF16">
        <property type="entry name" value="REPLICATION PROTEIN A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036949">
        <property type="entry name" value="RPA32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OB" evidence="4">
        <location>
            <begin position="53"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="226" mass="25994" checksum="E5E563FD38167332" modified="2012-10-03" version="1">MDRSDGGYIQSSPKREFLGIKTLRALTIKQINNCEFDEISSAHLIDSAEVTNVQIIGWVVSSRISATGSMFVLEDGTGSVDCTFWPSNSYEEEQCKILEEKNLFKVNGSLRTFNGKRSVSVSHLSQVEDSNFITYHFLNCIHQHLFYTRQLQREEVRSDGARLDRIQEDILECYRKNQDENGLHINVVIKMLSSKYPENEIRENIDVLLRDCHLYSVDGLEYKTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS50</accession>
    <name>I7AS50_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Diphthamide synthase subunit DPH2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264719.1">
        <property type="nucleotide sequence ID" value="XM_009266444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS50"/>
    <dbReference type="GeneID" id="20521528"/>
    <dbReference type="KEGG" id="ero:EROM_061320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061320"/>
    <dbReference type="HOGENOM" id="CLU_068933_0_0_1"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="317" mass="36945" checksum="FF980CAD4C357F75" modified="2012-10-03" version="1">MDFNLLDERLEGKTEVGLLSTEEYKYMVKDVYDHIKTKHPQVDTFLLNGEGMLCPLKTKTFQFFILLGVCCPLHSFEDFISISSHMSQEDTSLLKGEKRNVIYDSTYGRRSSENEMSGDILVVTKNQMFYEYYFYKYNAESFYNLERRDRMEYLSSKCNKGRRIRDLQIFAIIFTSRAYEELARSIRRALVSRRKNAYLLFLKDLSYERMITIEGTECIVVVDCPFFECTLDLHIPVVTPFEVEYAFSGEWKRFDKNSFSVSLESKSDCTEVDHIGKAGRILLRSEGQNVPYCYGEENMDIHSGDSGIPCKYDNEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS90</accession>
    <name>I7AS90_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264784.1">
        <property type="nucleotide sequence ID" value="XM_009266509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS90"/>
    <dbReference type="GeneID" id="20521595"/>
    <dbReference type="KEGG" id="ero:EROM_070360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070360"/>
    <dbReference type="HOGENOM" id="CLU_970096_0_0_1"/>
    <dbReference type="OrthoDB" id="2194465at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="31126" checksum="B4117ED6199EB969" modified="2012-10-03" version="1">MVFCMRGLWNIVPCRNKASEKVMKSTYLKTYETLQSEDDSNSLVDNDGMDEMLTKDSFRGMKSRGSERKMPRKIRKGASMSSEVKIDERKKQTTKSNSMTNSDDSWSDSYEDTFEKTEFRSSSKEKRKNRERYDKKESRSSRRVFSEDSSSGDAQGGRRSSKAMDMASKKSEILEKYRNRIYSGDKRHYNSDDISKESDMESSNYGKKERSTGSTSKQRPDEHKDSHNLDDSESEISFIMDIYDKSSLNNSLREKMFEYLKQMERKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASG8</accession>
    <name>I7ASG8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF155 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_071260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RMD1/sif2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264874.1">
        <property type="nucleotide sequence ID" value="XM_009266599.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASG8"/>
    <dbReference type="GeneID" id="20521686"/>
    <dbReference type="KEGG" id="ero:EROM_071260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071260"/>
    <dbReference type="HOGENOM" id="CLU_011220_4_0_1"/>
    <dbReference type="OrthoDB" id="18302at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003734">
        <property type="entry name" value="DUF155"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051624">
        <property type="entry name" value="RMD1/Sad1-interacting"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16255:SF1">
        <property type="entry name" value="REQUIRED FOR MEIOTIC NUCLEAR DIVISION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02582">
        <property type="entry name" value="DUF155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="308"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="DUF155" evidence="3">
        <location>
            <begin position="112"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="336" mass="39006" checksum="5025A8C92BD53443" modified="2012-10-03" version="1">MAEDCNKQKVYLTDISREPNAGGRKQARIELRALKDAYALKIEPDMMPKEESTVPDTNLFRVTAYCTADGYDLKNLYKYLKKNSLCNKVSMYFGECLYTLMNFRGSEERHDCFFYEYGVVVCWGMSETQESMIVKLVEKYEENGHEPSEVEIESFKYGITGNPFIINDVIYLNSENHFTKMVISIAIAQSVKLDYFENLVDNTIEAVKEFPEEVEREGKVSKSKKEILKMIGKLHRLRFNLNLGSNILDEPEFVWDYPAFSSLYETCKRYLDIKPRADLLNRRCDVINGILEILNENTNRSSIEKFEVVLIIIVLSNIVLIISQLIEMYIRLSSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASH2</accession>
    <name>I7ASH2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_071310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264879.1">
        <property type="nucleotide sequence ID" value="XM_009266604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASH2"/>
    <dbReference type="GeneID" id="20521691"/>
    <dbReference type="KEGG" id="ero:EROM_071310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071310"/>
    <dbReference type="HOGENOM" id="CLU_098695_0_0_1"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12276">
        <property type="entry name" value="RRM2_MEI2_EAR1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="108"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="253" mass="29828" checksum="2ABFD001466A51B9" modified="2012-10-03" version="1">MSRSGQGDQGQLTKTIIVTGFNDQKHQEEVKDRIKKKFEIKEIYTIQNDYRVLCILFYDERRAREAISYLKGSEDLSSYHIISKYEIPRDMDKCDESRNQSTLLFTFKNLTGSVDDKEFSEEVSKFGEVKDIRYVKTHQRCVEFYDSRNAIAAFHGMNELKFMDGVIQAKWVWDLSTKTRWELIRLTDSILKECKLEIKTPAKRESEDSREEMKKKRRAQDNGGMHMKNIFLQKFDAFIASNIDYISEGYIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASK0</accession>
    <name>I7ASK0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264909.1">
        <property type="nucleotide sequence ID" value="XM_009266634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASK0"/>
    <dbReference type="GeneID" id="20521725"/>
    <dbReference type="KEGG" id="ero:EROM_071610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071610"/>
    <dbReference type="HOGENOM" id="CLU_328190_0_0_1"/>
    <dbReference type="OrthoDB" id="995477at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt6 SH2" evidence="1">
        <location>
            <begin position="719"/>
            <end position="890"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="893" mass="103312" checksum="0E52566238C1FC01" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASK9</accession>
    <name>I7ASK9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Metal-dependent protein hydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264919.1">
        <property type="nucleotide sequence ID" value="XM_009266644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASK9"/>
    <dbReference type="GeneID" id="20521735"/>
    <dbReference type="KEGG" id="ero:EROM_071710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071710"/>
    <dbReference type="HOGENOM" id="CLU_051576_0_0_1"/>
    <dbReference type="OrthoDB" id="10265310at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003226">
        <property type="entry name" value="MYG1_exonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="36170" checksum="6810027A5FB0DABC" modified="2012-10-03" version="1">MILVTHDRKFHLDEVLATAVLLKIYPDSEIIRTRNPAVVQGGDIIYDVGGVFDPKTNRYDHHQESFNETFSSNHKIKLSSSGLIYKYYGERFLEVYGITRTDEYFHKALEEIYETYFMSADAIDNGYEIFGEIVPRSLSHIVESFNILSFSGNENDEQNRRFLEAVRIVSRDLDNFMHTMIGSWIPNYKYLDKLISGVVGDILCVDRYCFIDVVPEIEKKYKKDIKFVLNERENSVTILAVPKERKHFKSKIPLKKEWRGLTGSKLETISGIEGCNFVHASGFVGSNKTIEGALEMCRVSAEAYCREIDVPSNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASP1</accession>
    <name>I7ASP1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264954.1">
        <property type="nucleotide sequence ID" value="XM_009266679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASP1"/>
    <dbReference type="GeneID" id="20521771"/>
    <dbReference type="KEGG" id="ero:EROM_080350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080350"/>
    <dbReference type="HOGENOM" id="CLU_1015730_0_0_1"/>
    <dbReference type="OrthoDB" id="2194664at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="268" mass="30300" checksum="2634A71E80F01B9F" modified="2012-10-03" version="1">MSISVRMAKAKENLKDLHEKTNEACKRIKERIKENHKSINICGIEIDSSDVLSLILIFTPIVPSILGFNNSYVPTTISIIGYLVAIIFSLYQGITKLYNSYVKYKETHSIWELLGFRNLMLLFQVAFGMFLSLHLLHITDKHRTESIGTYGLMISLVYISKLAWKLSNFSIKKDDFYQFLILLIGLVLILLATSIAWKEVTTSYIVVSALLSSMMILSDHIGEPITEKLDETEPLSAIAALLPIFTVIYVTANTIQKFKIPFQGSQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATI0</accession>
    <name>I7ATI0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunitalpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091460</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265259.1">
        <property type="nucleotide sequence ID" value="XM_009266984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATI0"/>
    <dbReference type="GeneID" id="20564370"/>
    <dbReference type="KEGG" id="ero:EROM_091460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091460"/>
    <dbReference type="HOGENOM" id="CLU_029393_5_2_1"/>
    <dbReference type="OrthoDB" id="10256198at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02000">
        <property type="entry name" value="TPP_E1_PDC_ADC_BCADC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050642">
        <property type="entry name" value="PDH_E1_Alpha_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF60">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Dehydrogenase E1 component" evidence="4">
        <location>
            <begin position="45"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="351" mass="40043" checksum="B3007D37CC44619C" modified="2012-10-03" version="1">MQEKRYIHCDAQGILNSIQTHGITKEEIDIVQKLEVDEMVYIYKQMMKIRLTDEIMDREYKEKSIRGFCHLSIGQEGIYAALEHELDGDMVISSYRCHGLAYVTGCKTLEILGEVLGKQAGVCKGKGGSMHLYNRNFFGGHGIVGAQVPLGLGISYALKYKGRLLQDRDQGNRVCYAFYGDGAANQGQVWESFNMAMIWRLPIVFVCENNRYGMWTSAESISTDTNFYLRGGTMPGIRIAHNNIFGLISVLRYARRYSSEKGPIIVQIDTYRLCTHSTADERESYRAKGEIDNERKNDCMEDTRNRLLAFCSREEIDAIKDRVLEEVERDVDTAKKSKPTEESELFKDILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATN5</accession>
    <name>I7ATN5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kinesin A-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_100200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265334.1">
        <property type="nucleotide sequence ID" value="XM_009267059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATN5"/>
    <dbReference type="GeneID" id="20564450"/>
    <dbReference type="KEGG" id="ero:EROM_100200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100200"/>
    <dbReference type="HOGENOM" id="CLU_001485_12_4_1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47972:SF28">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KLP-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="171"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="53"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="246"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="497" mass="56189" checksum="916C964A2FDC54AC" modified="2012-10-03" version="1">MVRPGIQHRIDEIKEVFSEIKENIDGNPSSRIRKDSILLEICTPGTCGYNGCSRSTRNRESELLERIEELESAIERLKQASDSNINSRQSNVEDINAMLQGKDSEIQRMKEELSRILSMNDHLQRVNSEMQCRLNMYKEKASKLEEIKKECLEHKETISKLRNEIMVLKGSIQIICRIRPNTPNHHGSRIEISDGYLRISTDSKEHGFSFDKVFGPHATQKCVYREMESTFQSVLEGYSVCVFAYGQTGSGKTYTMEGVDGDPGLIVRALKDVYCIIEELKEDGWGLDITCSYVEIYNEDVVDLFSEDMKKVTIVHKDVNINMNCISVPIHNISDAIRLFQDGARRRSVGSTKCNSKSSRSHAVYILRIKMNNEALKQQKEGAMVFVDLAGSERLSVSRAEGIRLKETQSINKSLSALGDVFNSILRKDSHIPFRNSKLTHLLQSFLSGNSRTIMLVNISPDADHFSETICSLRFADKVGQCKLGSIRRKVTSIVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATY5</accession>
    <name>I7ATY5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histone acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_101320</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family. GCN5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265444.1">
        <property type="nucleotide sequence ID" value="XM_009267169.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATY5"/>
    <dbReference type="GeneID" id="20564562"/>
    <dbReference type="KEGG" id="ero:EROM_101320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101320"/>
    <dbReference type="HOGENOM" id="CLU_015741_1_1_1"/>
    <dbReference type="OrthoDB" id="1937912at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000123">
        <property type="term" value="C:histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010484">
        <property type="term" value="F:histone H3 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037800">
        <property type="entry name" value="GCN5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750">
        <property type="entry name" value="GH11602P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45750:SF3">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7 12" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="5" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="14">
        <location>
            <begin position="35"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="13">
        <location>
            <begin position="300"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="401" mass="46810" checksum="4738D38C43D1C739" modified="2012-10-03" version="1">MGGIGMDIFGLENNQARKDGNEMCQKFKDRELKCAIVSSSDPQVDKILLLRIKCLFQRQLSKMPKEYILRQVFDTKHANMVLVNSAEEVVGGICYRPFFERGFVEIVFLAVDYDFQVKGVGGFMMDLFKEVIKEEIKSCKWKFADNVLGTYKHEYRIIEDLSPLLNKMFENDSGPFYLVTYADNFAIGYFRKQGFTTEMEFGGWIGFIKDYEGGTIVECHVFWEINYLKKQEIIGNIKKKLFEEMKKINTYHIVHKIDDYSRIKSIEDIPGVLDAKYVSRENSDLKCQARFISYLISDLQVSPYAWPFLKPVDPVEVPDYYKQISNPMDLSTMMSKLKNNEYKYVESFVRDMNLMVNNCFAYNGKDTQYHKCAQILLGHFNKKLEIYKHVIDRLPKERGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUA8</accession>
    <name>I7AUA8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Synaptobrevin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_110850</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265564.1">
        <property type="nucleotide sequence ID" value="XM_009267289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUA8"/>
    <dbReference type="GeneID" id="20564684"/>
    <dbReference type="KEGG" id="ero:EROM_110850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110850"/>
    <dbReference type="HOGENOM" id="CLU_107225_0_0_1"/>
    <dbReference type="OrthoDB" id="1719357at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Longin" evidence="5">
        <location>
            <begin position="7"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="211" mass="24254" checksum="B81306DEDC27288D" modified="2012-10-03" version="1">MAILYTQIIKFPDYRILSGEYSPQSNSLRPGNEVVKELRDTIKSVPSSEAMSYYTFNSSDTRFVFYFKISSGIVFAVISDRYTSSKLASGYIDQVAEAFGKIYVDNPKATYYTFDPTIKTISDKFNRDSNYAQGMAVVEETKGLLAESLNMIIKRDENINNLKGLASRMTTEAQMMQKNIQRMHLKSMLNDYWIYVVFAVLIVLFLYYITH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUK7</accession>
    <name>I7AUK7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cysteine desulfurase, mitosomal</fullName>
            <ecNumber evidence="3">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_111660</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters in mitosomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Mitosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="3"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265644.1">
        <property type="nucleotide sequence ID" value="XM_009267369.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUK7"/>
    <dbReference type="GeneID" id="20564765"/>
    <dbReference type="KEGG" id="ero:EROM_111660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111660"/>
    <dbReference type="HOGENOM" id="CLU_003433_0_2_1"/>
    <dbReference type="OrthoDB" id="10250117at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990221">
        <property type="term" value="C:L-cysteine desulfurase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032047">
        <property type="term" value="C:mitosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000003">
        <property type="entry name" value="Cysteine desulfurase IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010611">
        <property type="entry name" value="PRK14012.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601">
        <property type="entry name" value="CYSTEINE DESULFURYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1025">Mitosome</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="15">
        <location>
            <begin position="33"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00054642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00067121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00084113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN84147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="431" mass="47087" checksum="EDA278FC31FB5B18" modified="2012-10-03" version="1">MIGGLKNYVEKPTLPSPTLPHHPLSRNSAQKGIFFDVQSTTPVDPRVLDAMLPFYTTVFGNPHSRTHRYGWQAEAGVEKARSQVASLIGCDPNEIIFTSGATESNNLALKGVSGFKLKEGKPVHIITLQTEHKCVLDTCRNLEENGIEVTYLPVGKDGVVDIEDIRRNIKENTVLVSVSAVNSEIGTIQPLGEIGKLCKEKGVLFHTDAAQGAGKMKIDVNEMNIDLLSMCAHKIYGPKGIGALYVRRRPRVRMIPLINGGGQERGLRSGTVASPLAVGFGKAAEICSKEMKRDFEYIKKLSDKLKSIFKKNIEGVIINGSEDGNPGCVNVSFPFVEGESLLMYLKDIALSSGSACTSASLEPSYVLRALGRDDEVAHSSIRFGIGRFTLPQEVDVVASRTIEAVRKLREMSPLYEMVKEGIDLSKINWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUL1</accession>
    <name>I7AUL1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111710</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265649.1">
        <property type="nucleotide sequence ID" value="XM_009267374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUL1"/>
    <dbReference type="GeneID" id="20564770"/>
    <dbReference type="KEGG" id="ero:EROM_111710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111710"/>
    <dbReference type="HOGENOM" id="CLU_594507_0_0_1"/>
    <dbReference type="OrthoDB" id="2193331at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="263"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="19"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="82"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="460" mass="50049" checksum="E57D7DE65143BB1F" modified="2012-10-03" version="1">MNSNFFNQQNKDDNNQKNPFLNTPFGSQQNTFGNQVNKPNIFGGQSQQTGAFGTQPTGGMFNSAPPAGFGGSGQGFSAQGFLNKQNPSTGFNVQQPQPSTSLFGKSSNLQPNGSSSIFGGQSTLQPSESPFKPQPTAPSSGVFGTQQLNNTFGTQFQPQTGNAFGTQAGGTSQPVNPFASSIQDPLSTGIRSTQDQGNVESSRMASGNGFGASQSSGERQNLGPQMQQAIPSNPQASGPFSTSVHTAASTLGTQQNIPFQFTPAQESQPGASSALPSPVAPLGSEPLSQGTEARDADSSVRKARGSASVFNQGVGEKGVSFMQMTLGEIIQQQTRKLEENIRVFKEKAKEVFEQDERIIRSLNNYKLIRNRIEEEERVIAETEENVEFFEKWLCNFQEEVPEGSEDELLMCIKEFERVCDKYNKTIETLKDEEDEVMCLVNENYNLITTIEEKLEILERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH11</accession>
    <name>I6ZH11_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263975.1">
        <property type="nucleotide sequence ID" value="XM_009265700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH11"/>
    <dbReference type="GeneID" id="20520761"/>
    <dbReference type="KEGG" id="ero:EROM_020030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020030"/>
    <dbReference type="HOGENOM" id="CLU_474891_0_0_1"/>
    <dbReference type="OrthoDB" id="2190100at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="584" mass="68070" checksum="ECEEEC8FD431940C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHV6</accession>
    <name>I6ZHV6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase II transcription initiation/nucleotide excision repair factor TFIIH subunit SSL1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264290.1">
        <property type="nucleotide sequence ID" value="XM_009266015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHV6"/>
    <dbReference type="GeneID" id="20521088"/>
    <dbReference type="KEGG" id="ero:EROM_040240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040240"/>
    <dbReference type="HOGENOM" id="CLU_028556_0_0_1"/>
    <dbReference type="OrthoDB" id="284275at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007198">
        <property type="entry name" value="Ssl1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12695:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04056">
        <property type="entry name" value="Ssl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ssl1-like" evidence="1">
        <location>
            <begin position="48"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="340" mass="38586" checksum="821B9D137AF99425" modified="2012-10-03" version="1">MAKQFSWEQEYKRTWEDRSDKKVHEFNMEAETFYSNNRKGIVRHLHVIIDVSEAIDKSDFLPTFRTNVARILEEFIPSFYNENPLSTLSFLSTRDVCVKYISSMDMDVHAFLGQTGSKWFSLLNGLEGSIEIMRNTMHVKEILVIVASTSTRDPHGYTEVLNKLKTYNIKVHFISLCGELTLYKSISKATGGRFYVPVDVGHLSMIMKELSHPTDFNGTTLSLVKIGFPLPTIESSVCSCHLEMKSIGYECPVCKTMVCSLPTSCPICGTQLVSTLNLSKSLRFLYPLKPFIEKAEGICRICRDKGAYQCELCKSTFCSYCNGLAHNTLSFCIYCELPHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHW1</accession>
    <name>I6ZHW1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L14E/L6E/L27E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_040290</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264295.1">
        <property type="nucleotide sequence ID" value="XM_009266020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHW1"/>
    <dbReference type="GeneID" id="20521093"/>
    <dbReference type="KEGG" id="ero:EROM_040290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040290"/>
    <dbReference type="HOGENOM" id="CLU_067359_2_1_1"/>
    <dbReference type="OrthoDB" id="2365484at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06090">
        <property type="entry name" value="KOW_RPL27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001141">
        <property type="entry name" value="Ribosomal_eL27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041991">
        <property type="entry name" value="Ribosomal_eL27_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038655">
        <property type="entry name" value="Ribosomal_eL27_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10497">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01777">
        <property type="entry name" value="Ribosomal_L27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="126" mass="14507" checksum="5085B6519FF66BFA" modified="2012-10-03" version="1">MIFVPNMIVVVTSGKNAGKKAVVIKQLDERYVLTACVVRVPKDSEDHQPKWIKRRNSKFYVVLKKYNISHLIATRYKADLGLSSIDYSGIEQNAETKRVLTDKTKDFMMKAWKESKAKFLFSSLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI07</accession>
    <name>I6ZI07_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264345.1">
        <property type="nucleotide sequence ID" value="XM_009266070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI07"/>
    <dbReference type="GeneID" id="20521144"/>
    <dbReference type="KEGG" id="ero:EROM_040800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040800"/>
    <dbReference type="HOGENOM" id="CLU_090707_0_0_1"/>
    <dbReference type="OrthoDB" id="2188983at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="31334" checksum="65822C112B692C11" modified="2012-10-03" version="1">MRLSLDEQKDVMKEFTDPVEFIRGYIDVYEKQRSVPVKVYLEDISYYERFEPMFLDLVLGKALSEGPDLNFPEVEELLQTFCNKEFYDERFYLESTLVLIKGIAVLIDRVDQEVQRRKFDNVQYLYYYTTEPIDLTRVLVDPYTRYIQDPPTLVSKMAELREIVEFVNKQLEGVGNSFLVNDKRLKERMNLSDGILGQKRIEKYKIEDVYGSIFDLLMVRATGMDVESGYIYIMGFCSEFLMSEVGDEKAILCLKEYAGGLLNKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI48</accession>
    <name>I6ZI48_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264395.1">
        <property type="nucleotide sequence ID" value="XM_009266120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI48"/>
    <dbReference type="GeneID" id="20521195"/>
    <dbReference type="KEGG" id="ero:EROM_041320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041320"/>
    <dbReference type="HOGENOM" id="CLU_1151788_0_0_1"/>
    <dbReference type="OrthoDB" id="2193088at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="241" mass="27198" checksum="1BD0D66BD08F1DAF" modified="2012-10-03" version="1">MDNGKRHKTETPVENSDEILEEGMSESYSVSEVSSCDEISYEVTDPSSSNLSGISTIMEQSKIFPFSEEFLSSFTISKVLTSGDTILSFCGFLQLSRMLGIPQKKLKKALNTLISTSGSKEIIKDKVYVFCTERAANIPLEMILDLYKSLSFIDFKYMVFVSRVKVCGKSEAKDLMEDFKEYDPSDLSYIPVRGEEILLLSSYIAKKQIVVNGNTFRLFLINGRMFESFIKLFEKELSEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI66</accession>
    <name>I6ZI66_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methionyl-tRNA synthetase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_041540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264415.1">
        <property type="nucleotide sequence ID" value="XM_009266140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI66"/>
    <dbReference type="GeneID" id="20521217"/>
    <dbReference type="KEGG" id="ero:EROM_041540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041540"/>
    <dbReference type="HOGENOM" id="CLU_062267_0_0_1"/>
    <dbReference type="OrthoDB" id="19141at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051270">
        <property type="entry name" value="Tyrosine-tRNA_ligase_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586">
        <property type="entry name" value="TRNA-AMINOACYLATION COFACTOR ARC1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11586:SF37">
        <property type="entry name" value="TRNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 3" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="4">
        <location>
            <begin position="124"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="285" mass="32064" checksum="C81DEC64C01E129E" modified="2012-10-03" version="1">MKLEFSDSFRDLAFVLRYFNRDANLEKVESGLKLFNGDEKHEGLDDVLGALYKMYEQDCDYKNIKEAKKLRANPEVRKVEVSISNLISFYGLYCQMITQGVLKNKLFIDQMVEKIAPCMKLDPDFQLLDIQVGEIVNIGDVQGLDKLYSEEVVGKKKMQILSGLRGHVRKEGLLGNKFLFVTNMKPAKFKGQVSEGMILCVKDKDGNIEPIEISKDIINGSRLELEGFDTILEDFSPGKVDMRKSGYAHALESFKVVGHFLTFKGVRVTCGGKPIKTNTSDGPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIC9</accession>
    <name>I6ZIC9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Inorganic pyrophosphatase/exopolyphosphatase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_050450</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264475.1">
        <property type="nucleotide sequence ID" value="XM_009266200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIC9"/>
    <dbReference type="GeneID" id="20521277"/>
    <dbReference type="KEGG" id="ero:EROM_050450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050450"/>
    <dbReference type="HOGENOM" id="CLU_059048_0_0_1"/>
    <dbReference type="OrthoDB" id="374045at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF22">
        <property type="entry name" value="MANGANESE-DEPENDENT INORGANIC PYROPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="5">
        <location>
            <begin position="26"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA2" evidence="6">
        <location>
            <begin position="246"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="397" mass="45208" checksum="150CCA61D2DF5485" modified="2012-10-03" version="1">MALLKEKLKLFFEVNKEKIHCEEVLVAMGNEACDLDSFISSLVVAYAEGAVHVVNMRKEVFVAKGEIMWVCKEFGIDVDDLIFLVKPTLHFSSKARKIGAYFDSGEKEYPVCGKKIKLLLTDHNQPAEELEDCEIELIIDHHMVERSVSPAKRIYIDLDVGSATTLVSKYLGEDLARKNHSLPSFTKSQKDKETLCSAFAKLLLIPILMDTGFLKKRTSVFDMAEYRRLKRLADVKKKKLKGIIRALKNARKNDSGQETGLILQKDFKTFHYRDFFFGGSTVKYPFEDWADREGRNISRIPEEKAGIALMVEIESFRKAMGLDFFFVATKAKGSRNIIFANFPFARQIAAKNQMKSIDYKGLEYYSASKNLTRKLLVPEIIKAIDGYTHGPNKNKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIK0</accession>
    <name>I6ZIK0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transitional endoplasmic reticulum ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_051170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264545.1">
        <property type="nucleotide sequence ID" value="XM_009266270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIK0"/>
    <dbReference type="GeneID" id="20521349"/>
    <dbReference type="KEGG" id="ero:EROM_051170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051170"/>
    <dbReference type="HOGENOM" id="CLU_000688_8_5_1"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000567">
        <property type="entry name" value="ATPase, AAA family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="70"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="295"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="506" mass="56620" checksum="711249AB4711FB70" modified="2012-10-03" version="1">MNRKNPMRDAGSALRDKYITSHLNESLKIDHEYPPLQKVGGIKYLLPKITELVYNPLFSKASYDEIGIHPPSTLLLHGVSGVGKTFLVNCISQEYKLPIIRASLDSDKELRESFRKSSLLERSIILIEDIDVLGEDDKKVISQLSEYLENHKGKSIVIATARNPAFINESLNKFDNSLLVKIPTAAERREILISLTDKMKCQETDWTELAKLMPGFVARDLKRALKIASTKAVADGSSAIRMIDLEVALKEMKWKGAEITFDSIGSLEDVKDELNMSIIFPSRFPEKFRKLGITRPSGILLYGPPGCGKTLLVRAVSNMSHCNFLSIKGPELISKYVGDSEKEIRKLFDKAKQLQPCVLFFDEIDSLCGERNGNEFTSRIVNQILTLLDGLDDRGDVYVIGATNRIESIDKAILRPGRFDKILKVPLPSKEGCVDIFRKCTKGIPIEPFDFEVLNLEGFSGAEIAGLIRDAAILCLKHNFDSDSLVITKENIIEALEAARVTKDFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIM1</accession>
    <name>I6ZIM1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">U6 snRNA-associated Sm-like protein LSm1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LSM1</name>
        <name evidence="8" type="ordered locus">EROM_051380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the cytoplasmic LSM1-LSM7 complex which is involved in mRNA degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the heptameric LSM1-LSM7 complex that forms a seven-membered ring structure with a donut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264565.1">
        <property type="nucleotide sequence ID" value="XM_009266290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIM1"/>
    <dbReference type="GeneID" id="20521369"/>
    <dbReference type="KEGG" id="ero:EROM_051380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051380"/>
    <dbReference type="HOGENOM" id="CLU_076902_8_4_1"/>
    <dbReference type="OrthoDB" id="10263346at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01728">
        <property type="entry name" value="LSm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034104">
        <property type="entry name" value="Lsm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044642">
        <property type="entry name" value="PTHR15588"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588">
        <property type="entry name" value="LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15588:SF8">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU365047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="107" mass="12571" checksum="5B36561B12212273" modified="2012-10-03" version="1">MLDEDFGNTRDYEKYLEQEIVVVLRDGRYLYGIMKSFDQFNSITLDRVIERIFHDEKYAERKHELFIIRGENITMIGLGPSDIGRGLVQVDFLVLRNEILSSQLQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIN4</accession>
    <name>I6ZIN4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_051530</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264580.1">
        <property type="nucleotide sequence ID" value="XM_009266305.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIN4"/>
    <dbReference type="GeneID" id="20521385"/>
    <dbReference type="KEGG" id="ero:EROM_051530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051530"/>
    <dbReference type="HOGENOM" id="CLU_052673_0_0_1"/>
    <dbReference type="OrthoDB" id="2189411at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF4">
        <property type="entry name" value="LYSOPHOSPHOLIPID ACYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="394" mass="45990" checksum="71603E1C56964CA9" modified="2012-10-03" version="1">MIRKISEVNYLERRFLASLFLTLFVSYTMRNRARAHNLLSAMLILYVAFDHKHTAYILASIALNIFLLKYVPMTEYSFTLVNIMVLYVYKIFGGLFEERISGSFDISGVLMLMTIKMCYLGKEYNKRTNTIKDALSYILFIPGLMLGPVPTFKDFMENKYEKPKKPPYETFLKSFGFLILFQALRVSVPRSHLLEENISLPMRLVYIYLFTVGNRIKFYFAWHFSHGCFTFQNFPNLLNADFIKVELATSVKDLSTYWNICTGVWLKNCFFVPMKKRSIFWASLATSAVSALWHGINPCYLVMFLSLSISIPVVKENNRLIQHFFPSLFWILSRVQMLLLTTYFTASFFLLDISDLIYVWRSVYFAGHIVLAFSLIVQTAIKFFSPPVEKKRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ01</accession>
    <name>I6ZJ01_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264675.1">
        <property type="nucleotide sequence ID" value="XM_009266400.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ01"/>
    <dbReference type="GeneID" id="20521482"/>
    <dbReference type="KEGG" id="ero:EROM_060860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060860"/>
    <dbReference type="HOGENOM" id="CLU_384954_0_0_1"/>
    <dbReference type="OrthoDB" id="2195025at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF10">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="224"/>
            <end position="719"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="720" mass="82726" checksum="CF9CB49D50244295" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ04</accession>
    <name>I6ZJ04_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Class 3 adenyl cyclase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264680.1">
        <property type="nucleotide sequence ID" value="XM_009266405.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ04"/>
    <dbReference type="GeneID" id="20521487"/>
    <dbReference type="KEGG" id="ero:EROM_060910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060910"/>
    <dbReference type="HOGENOM" id="CLU_1050098_0_0_1"/>
    <dbReference type="OrthoDB" id="2191719at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009190">
        <property type="term" value="P:cyclic nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050697">
        <property type="entry name" value="Adenylyl/Guanylyl_Cyclase_3/4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081:SF1">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43081">
        <property type="entry name" value="ADENYLATE CYCLASE, TERMINAL-DIFFERENTIATION SPECIFIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707017" evidence="1">
        <location>
            <begin position="17"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate cyclase" evidence="2">
        <location>
            <begin position="106"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="30875" checksum="2BAEA74B9F93CB9F" modified="2012-10-03" version="1">MWSNSMILMLQLMGYAIKMNTQMIVYIIHVVVNPVRAFGSVFCGFKVGIVDGVRCMLVPRSKEKESQEARKAILLPKNMEVLSDNLCYKKYIPQKIHKRFLQGDLVLVITDIVDSTSLWNCFPDAMHRTIEVHDEVARGLCKAYGGLEVRNEGDSFFLVFTDIRNALDFSIRFYREIECISFNFRHAFTKRVREGKETEFLPLKIRMAMNKGPIMLCHNKFLEIYGDAVTKTFGMLSHSNGKICISQNCLEPGNSWNRRKRLFCIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ95</accession>
    <name>I6ZJ95_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264805.1">
        <property type="nucleotide sequence ID" value="XM_009266530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ95"/>
    <dbReference type="GeneID" id="20521616"/>
    <dbReference type="KEGG" id="ero:EROM_070570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070570"/>
    <dbReference type="HOGENOM" id="CLU_1288889_0_0_1"/>
    <dbReference type="OrthoDB" id="2190706at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="214" mass="25120" checksum="5E1FBA331CC0A2A7" modified="2012-10-03" version="1">MENEEEIEFFGFVPVTLVAELQSEIEGILRDGVEQLSFLDKRKAHRISGIVFESFRRNYFIFSNFVLRNILRFPPSFRLERKANDAVVTIDLQSITDELVNVLGEEDYYEAEVLRLKESIDIERYRLESYRSLLECSKPVNSLIESIMEAYSELENVTKLYDKMSMISGMDDEDHNALLEYREIRSSLAKKERDDLLRIASEEVLMMMNKCTEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJC3</accession>
    <name>I6ZJC3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264835.1">
        <property type="nucleotide sequence ID" value="XM_009266560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJC3"/>
    <dbReference type="GeneID" id="20521647"/>
    <dbReference type="KEGG" id="ero:EROM_070870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070870"/>
    <dbReference type="HOGENOM" id="CLU_075869_0_0_1"/>
    <dbReference type="OrthoDB" id="2190927at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="310" mass="34811" checksum="614B334A70F7CF8C" modified="2012-10-03" version="1">MLLSLVFPVMVLGEKFDGYGPTAFDVSLSPDIPIEELTGYGTKHTKERGNKGFGDLSLVDFNMETEDSFDRNKLVDIAWNILDSSHGTVMAMQSIRKGEADALSIKTLGHYEMLNLSEGSSNVQEAPITYLPIVFDTLVLDHKKSGYVRDPENSKIIYASWAIFEHKKTKRWFTVVNVDMYSAFSEKTDVEMANLLEDMNKEHTVESNPVFFMGRINAISEKLQKVIDDKYTNPLDADKNAKEGPRFTMKNLPDILGNFQRDFVLVRDATSQVVKINYARILRKGISTLHYPIHVIASFGGSQNSTPPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJD7</accession>
    <name>I6ZJD7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_071020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="5">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264850.1">
        <property type="nucleotide sequence ID" value="XM_009266575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJD7"/>
    <dbReference type="GeneID" id="20521662"/>
    <dbReference type="KEGG" id="ero:EROM_071020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071020"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_4_1"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="56"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="267"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="416" mass="46956" checksum="0B834655E53FCDF6" modified="2012-10-03" version="1">MEDVLLKGIKQKRKKTPKEEVGYDVVGERYPLLCTFESLETKVPFESPLLVQRIVIPYFLKKTNVVVQAFTGSGKTLSYCLPLVEMARKESLFGMILVPGKALVRQVYRVVNEIKHEGLEVVAGYRDGEEDFLIEEDEKGGLAERGVDCRSKEKIERAFQSDAKILIGTPLSALGLMPMSGTRSITHLVIDEADLLINPGTVEVFGSILREIEVENAHIGCFSATMNEYVGEVIETVKDITKVYVMSKRLIDHEFVFGTSKKIKHLSLLQIIADGIESPTLIFVKDEKTGEFLKGLLDRSGVYKEGGEVSGEVLDAFRLKKIWYLFGTDSLSRGVDFYNIRSIINYDLPDTKTQFVHRVGRINRNCQGQKVYTIYTSEDFGRISIVAEFLEENGHEVPKHITRIIEKSKKKMSKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJJ3</accession>
    <name>I6ZJJ3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">40S ribosomal protein S2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_071620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264910.1">
        <property type="nucleotide sequence ID" value="XM_009266635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJJ3"/>
    <dbReference type="GeneID" id="20521726"/>
    <dbReference type="KEGG" id="ero:EROM_071620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071620"/>
    <dbReference type="HOGENOM" id="CLU_065898_0_3_1"/>
    <dbReference type="OrthoDB" id="10253125at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="S5 DRBM" evidence="8">
        <location>
            <begin position="63"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="242" mass="26292" checksum="9ABCCCFCBE550F81" modified="2012-10-03" version="1">MEGTEKRNPGRPFPRKEESEWVPRTAVGRLVKMFKITSLDQIFKFSHVIREPEIVDMLCGNSIKEEVLSIKSVQKQTRAGQKTRIKAVVVVGDENGHVGIGTKASKEAASAIRGAIARAKCAIRPVRLGLWDGNQGKPHTISCKGSGKCGTSIVRVIPAPRGTGIISGSVNKKIFELAGIKDLYTISSGATSTTENFAKASIAALEATSSMILPEQWEEKPRTLNPLLEFADILNQLDKSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJK6</accession>
    <name>I6ZJK6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sm-like small nuclear ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264925.1">
        <property type="nucleotide sequence ID" value="XM_009266650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJK6"/>
    <dbReference type="GeneID" id="20521741"/>
    <dbReference type="KEGG" id="ero:EROM_080050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080050"/>
    <dbReference type="HOGENOM" id="CLU_076902_11_4_1"/>
    <dbReference type="OrthoDB" id="429711at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="7"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="73" mass="8371" checksum="F9CFB342C34BD393" modified="2012-10-03" version="1">MELPALEILKKNRSKNVRIITLNNKTYKGVLENFDMHVNTHLKDSYLRVDGEEETYIGEVLINGGTIACFDIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJN6</accession>
    <name>I6ZJN6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IWS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264955.1">
        <property type="nucleotide sequence ID" value="XM_009266680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJN6"/>
    <dbReference type="GeneID" id="20521772"/>
    <dbReference type="KEGG" id="ero:EROM_080360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080360"/>
    <dbReference type="HOGENOM" id="CLU_106415_1_0_1"/>
    <dbReference type="OrthoDB" id="21124at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="102"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="182" mass="20517" checksum="D2200CBC61549351" modified="2012-10-03" version="1">MDKEGCQGYPEAGGGDSEGKDGRQKKPKTSEGLGRKGELDPEKALEVARCLKRRMKDILKKDNTNNLEGRPATGKIENVEEVSDILMNKGLQESLLDEGILNEVKGWLEPLPDRSMPNIKVKKRLLDVLKSMKIHKEHLIVSGVGKIVYFYSINPKETKEVRSMAKTLVQKWTNEVFKPEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK86</accession>
    <name>I6ZK86_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transmembrane amino acid transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_090560</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265170.1">
        <property type="nucleotide sequence ID" value="XM_009266895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK86"/>
    <dbReference type="GeneID" id="20564280"/>
    <dbReference type="KEGG" id="ero:EROM_090560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090560"/>
    <dbReference type="HOGENOM" id="CLU_009020_1_2_1"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF678">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="3"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="388" mass="43091" checksum="C08F7E509201CEC4" modified="2012-10-03" version="1">MATTLASGYITLLKTSIGSGILSFPYLFKTYGILIGIALTMLSGFFSVLGLILYAICSQELGRTATLSRLATESMPYTRIIVDFSVFLKCFGVALSYLIITRQLLPVLIETIFGVSILSDPNISLLAFISCIGPFAYFNKLDRLKYTSLCGVVAIVVVVIAALYRYEYTPASTPSRAEYFVPFSYAWMGGFGKFVFSFTCHQNIFAIHSEMEDNSFPRMKRLIYMVATSAAVIYISFGILNYLLYGDRVKDNVLENYPNDVLASVVRGLYVVVMGVSYPLQVNPCRNHLINIISFTQESRKKPLRFIVTTGIIILTCLLAISGMGLGIIYSVIGSTASTFMCLIFPALFYFNMNITKPKGLVIMSYIAFLFGVFVFTTSLFNIILHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKA6</accession>
    <name>I6ZKA6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">U2 small nuclear RNA auxillary factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_090820</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265195.1">
        <property type="nucleotide sequence ID" value="XM_009266920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKA6"/>
    <dbReference type="GeneID" id="20564306"/>
    <dbReference type="KEGG" id="ero:EROM_090820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090820"/>
    <dbReference type="HOGENOM" id="CLU_059852_3_0_1"/>
    <dbReference type="OrthoDB" id="423462at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089701">
        <property type="term" value="C:U2AF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009145">
        <property type="entry name" value="U2AF_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12620">
        <property type="entry name" value="U2 SNRNP AUXILIARY FACTOR, SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01848">
        <property type="entry name" value="U2AUXFACTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="7">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="259" mass="29455" checksum="D3C4E9D4B74F66C6" modified="2012-10-03" version="1">MVAGRDVCIFYSKTNGCRYGLECTKAHRIPARSKVVVVKNMYLYPKNDPDSKLDNEAVQIHLDLFYEDWFSEVSMKYGAVRMLAIASNSSLQLLGNIYIEFEDEKASLRCIEDIGKRYYSGKRIVVELGNCYRISDGVCTDYEKGLCGKGERCGFIHVAKVTESLMEELFASQELLYTQMDSMGKCKTSDGGKVTGQGKRAERSVEDGNNSGDYRMSRRRLGGQGDCIKRVREASNGSFGRYGWSDKDSRSRMYYYRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKF4</accession>
    <name>I6ZKF4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RWD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265265.1">
        <property type="nucleotide sequence ID" value="XM_009266990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKF4"/>
    <dbReference type="GeneID" id="20564376"/>
    <dbReference type="KEGG" id="ero:EROM_091520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091520"/>
    <dbReference type="HOGENOM" id="CLU_1722357_0_0_1"/>
    <dbReference type="OrthoDB" id="2191887at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17928" checksum="DC3E9C5D7B77A7F5" modified="2012-10-03" version="1">MDEHDALKYIFGSNLRHYAESTGQIYKISVGDKYFEFRCREDYPNSIPEVLSNVENEVLEGVIQKAERYVSTPMIFDIIRMTFRKLEESGIGKDTKRISACYSVDDDQRITEEDFLNWRKKTQRIVSRKSGRTGKEIFLERKRGKEEIQDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKL2</accession>
    <name>I6ZKL2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265335.1">
        <property type="nucleotide sequence ID" value="XM_009267060.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKL2"/>
    <dbReference type="GeneID" id="20564451"/>
    <dbReference type="KEGG" id="ero:EROM_100210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100210"/>
    <dbReference type="HOGENOM" id="CLU_112982_0_0_1"/>
    <dbReference type="OrthoDB" id="19329at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="218" mass="24669" checksum="08E1E35C9F047DF2" modified="2012-10-03" version="1">MKCSACQVEIGGNIKAHYASEVHKINVKRQIYNAPPITIEEINADQPSDDVSLEINGLDAEERSSRMCSKKRSPSRQGGTASGSVCLFCDLPEGTIHYKSHGLSDEEIAYINNRVCYVCYEAFSDYDGLRRHISSGNHRSAMTDGVNLILENGKVIQGRARSRVREEELIQRSERANRITVVKSLMKEDKKGIMKNKNQLKISLSMNYQPRFKPDWMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKV9</accession>
    <name>I6ZKV9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_101380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265450.1">
        <property type="nucleotide sequence ID" value="XM_009267175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKV9"/>
    <dbReference type="GeneID" id="20564568"/>
    <dbReference type="KEGG" id="ero:EROM_101380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101380"/>
    <dbReference type="HOGENOM" id="CLU_031002_1_0_1"/>
    <dbReference type="OrthoDB" id="10268090at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="3">
        <location>
            <begin position="17"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="389" mass="44200" checksum="413603AD256C1C2E" modified="2012-10-03" version="1">MFFFEASPSMEREYDFIIYGASGYAARYIIEAFKAENVRLALAARNISKIKDKAFPVYECEIDGIDKIASMTKILINCVGPYSRYGESIVKSCIRNGTHYMDISGEVYFFELIINKYHDEAARKGVYIINCCGFESVPSDIGVMYLRDMFDDAEIESVLKISNVVVNETTWESLLASVANFKEMKALREKRYGQGKKRRSQRIVRESSYQVIFRGIDYSIVRRSQELLESVGLHGAKYAAYLDVGGRVGMIKYWIFLWIVWFFSGFALGKRIIMNLSKLFAFGFIKSNPSFEEIRNSSFTIEIRARGEKNNEIVGKCLIISGPDPAYIMTSICLTQTAVVFLRSLNQRVRGTGVTLFRGGVITPACVLYNTDIIQKLSSKGIKFEVKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLF8</accession>
    <name>I6ZLF8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Puf RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_111620</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265640.1">
        <property type="nucleotide sequence ID" value="XM_009267365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLF8"/>
    <dbReference type="GeneID" id="20564761"/>
    <dbReference type="KEGG" id="ero:EROM_111620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111620"/>
    <dbReference type="HOGENOM" id="CLU_004017_8_4_1"/>
    <dbReference type="OrthoDB" id="668540at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010608">
        <property type="term" value="P:post-transcriptional regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07920">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033133">
        <property type="entry name" value="PUM-HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033712">
        <property type="entry name" value="Pumilio_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537:SF12">
        <property type="entry name" value="MATERNAL PROTEIN PUMILIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12537">
        <property type="entry name" value="RNA BINDING PROTEIN PUMILIO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00806">
        <property type="entry name" value="PUF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50303">
        <property type="entry name" value="PUM_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PUM-HD" evidence="4">
        <location>
            <begin position="188"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="252"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="322"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="391"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="428"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="repeat" description="Pumilio" evidence="2">
        <location>
            <begin position="468"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="529" mass="60995" checksum="D9A86939CA02657C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSI8</accession>
    <name>I6ZSI8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gal4 DNA-binding enhancer-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264068.1">
        <property type="nucleotide sequence ID" value="XM_009265793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSI8"/>
    <dbReference type="GeneID" id="20520857"/>
    <dbReference type="KEGG" id="ero:EROM_020960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020960"/>
    <dbReference type="HOGENOM" id="CLU_140492_0_0_1"/>
    <dbReference type="OrthoDB" id="3169036at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAC-A/B" evidence="2">
        <location>
            <begin position="19"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="150" mass="16781" checksum="8A0D6D17B44C86A7" modified="2012-10-03" version="1">MSRPLTADEAHVHKIIGSKVGLEEIEGIEGVSVITGNIRCSVENSVVYKIKGTESLLIFGKQENPIDFGKLKKIYEDNVRYTEREGHEASGLYSEVTDDQEKKEPSQASSESTDRKLSEEDTKLTSSQVKASRDDIIKHLLNPIMMLLMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSL4</accession>
    <name>I6ZSL4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="4 14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_021210</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="4 14"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264093.1">
        <property type="nucleotide sequence ID" value="XM_009265818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSL4"/>
    <dbReference type="GeneID" id="20520883"/>
    <dbReference type="KEGG" id="ero:EROM_021210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021210"/>
    <dbReference type="HOGENOM" id="CLU_008438_5_0_1"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23276">
        <property type="entry name" value="beta-trefoil_MIR_PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="576"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="601"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="626"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="280"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="400"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN82596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="670" mass="78121" checksum="485516E574B3A8CA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSM9</accession>
    <name>I6ZSM9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_021360</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264108.1">
        <property type="nucleotide sequence ID" value="XM_009265833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSM9"/>
    <dbReference type="GeneID" id="20520898"/>
    <dbReference type="KEGG" id="ero:EROM_021360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021360"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_6_1"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070985">
        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF0">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="4"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="308" mass="35000" checksum="4A443EFF3C57CC97" modified="2012-10-03" version="1">MKTYIKEKRLGEGTYAVIYLGYRALPQDKPFVNTGTRIEDTPVAIKRVKPTKYTQGHEISAIREIKALKRISSKYVVKLLDAFIHDKCVHIVLEYVETNLENVIRNTDKIIMPGDIKAWMLMILRGVYECHKLFIIHRDIKPNNILITAEGMVKLADFGLTRGIRNKMTPQAVTRWYRAPELLMGSRDYGSPSDMWSVGCVFAELFLRVPLFGGDTDIHQLDMIFRALGTPTEKEWPGVSTLPEFLEFQQYAKISLGSLFSAASSDALDLLERLLTLNPCNRINCDDAIKHPYFKSLPLPTPIGKLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT23</accession>
    <name>I6ZT23_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_031390</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264258.1">
        <property type="nucleotide sequence ID" value="XM_009265983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT23"/>
    <dbReference type="GeneID" id="20521053"/>
    <dbReference type="KEGG" id="ero:EROM_031390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031390"/>
    <dbReference type="HOGENOM" id="CLU_1982204_0_0_1"/>
    <dbReference type="OrthoDB" id="2191765at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15151" checksum="6014FF1A012F519C" modified="2012-10-03" version="1">MIILTILMSVGAYYMPSPRHAFIITTRTLSPYRLDSFPFSQVGPYKTSTDPILYSRPSRFETETSTEVGERKNSVSEEKKEGIKSGLSSIISTSMLCKLFFYTVMVVLIFFMGYQTRKCHEGGSYVRLPTSSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTE3</accession>
    <name>I6ZTE3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264418.1">
        <property type="nucleotide sequence ID" value="XM_009266143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTE3"/>
    <dbReference type="GeneID" id="20521220"/>
    <dbReference type="KEGG" id="ero:EROM_041570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041570"/>
    <dbReference type="HOGENOM" id="CLU_122961_0_0_1"/>
    <dbReference type="OrthoDB" id="2188863at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013198052" description="Thioredoxin domain-containing protein" evidence="2">
        <location>
            <begin position="16"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="191" mass="22617" checksum="767D8A6CA0E25019" modified="2012-10-03" version="1">MRIFFLLLGKVMSDAFSLLPVFTKEDVPLDSTFLVYIYYSRPNVHCPACESFNRNISKLQKYIPVKKINFFEDPKVASCFYSFIFPSFVIRDKGRSYHLDVDDFEELEEIVRNKRWTQFKPVRRWMDLDSYFINLYSTANCAFFKVMQKSYVVLDVIPGWIINLGFSAIIAYMVYSICTIFMTPVEKKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTS9</accession>
    <name>I6ZTS9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arginine/serine rich pre-mRNA splicing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_051510</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264578.1">
        <property type="nucleotide sequence ID" value="XM_009266303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTS9"/>
    <dbReference type="GeneID" id="20521383"/>
    <dbReference type="KEGG" id="ero:EROM_051510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051510"/>
    <dbReference type="HOGENOM" id="CLU_940185_0_0_1"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF62">
        <property type="entry name" value="SERINE_ARGININE (SR)-TYPE SHUTTLING MRNA BINDING PROTEIN NPL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="252"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="264"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="34298" checksum="3DF0A81E6CED88E7" modified="2012-10-03" version="1">MQIFIGKIPNHVNEEQIKEYFGQFGEVTDVSLKGTYGFLNFDSESSIARVLNQRSHTIDGAPISVERANGRKRPLDGEYHDRYMDMGRGGYSPHRGDYRGFRNAPYPPMRYDGRSPGRYDPRFGDRYGGRSPEYRGDHQRSRDFCEYCNSCPIHGMRDMMDPRKRVHASRDHPNNHLKVVFENIAPNTTIDDFKNFVHDHGFEPSYARLGYSGNHAVLEFKNIEDKDNVMKKLDGAEFNGHVLKTRSYLSKDEYKSRERESYMRNDQQPNENNSMEPQEAGTDIYEGIEDAKAEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTT4</accession>
    <name>I6ZTT4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L30</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_051560</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264583.1">
        <property type="nucleotide sequence ID" value="XM_009266308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTT4"/>
    <dbReference type="GeneID" id="20521388"/>
    <dbReference type="KEGG" id="ero:EROM_051560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051560"/>
    <dbReference type="HOGENOM" id="CLU_130502_0_1_1"/>
    <dbReference type="OrthoDB" id="1928736at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="10"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="108" mass="11736" checksum="70865880534CF031" modified="2012-10-03" version="1">MSGKSNKGDNVTSALLLAMRTGECIVGFKQAIKSVIMKRAKCLVISSNFPSTKRKQLEYYSVLAGGLPIIFHNANNEELGNITERGHRLGCISIIDQGEAELIPSRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU21</accession>
    <name>I6ZU21_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="3 13">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_060420</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="3 13"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264633.1">
        <property type="nucleotide sequence ID" value="XM_009266358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU21"/>
    <dbReference type="GeneID" id="20521439"/>
    <dbReference type="KEGG" id="ero:EROM_060420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060420"/>
    <dbReference type="HOGENOM" id="CLU_040369_0_0_1"/>
    <dbReference type="OrthoDB" id="10253702at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="15">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="14">
        <location>
            <begin position="167"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="302" mass="34404" checksum="BCFAA42F13E15794" modified="2012-10-03" version="1">MKDSSSGYGRTHPNIAVVKYWGKTDETKNTPSNPSISFPLINFLTETVIEYSMEDTFYLNNEMVTIGERMNRVVEIFRDRAGDGRPICIKSFNSFPHSCGLASSASGFAALALALNDFYNLETSEEELCRIARVGSGSAGRSICPEICLFNGIYIEKLPPWPEIRILSIILSGDCKKIGSTEGMIRTRYTSNLYQARLSGMEEKVKAMAQYITHKDFDGFAHLTMRESNELHSILMDTYPPIRYIRDDGFNIIEMCHEFNRDRVKIAYTFDAGPNPFLITLERYLNEVEDFFKAYKLIPCNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU77</accession>
    <name>I6ZU77_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Obg-like ATPase homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264703.1">
        <property type="nucleotide sequence ID" value="XM_009266428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU77"/>
    <dbReference type="GeneID" id="20521510"/>
    <dbReference type="KEGG" id="ero:EROM_061140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061140"/>
    <dbReference type="HOGENOM" id="CLU_018395_1_0_1"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF18">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="6">
        <location>
            <begin position="18"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="7">
        <location>
            <begin position="277"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="370" mass="42167" checksum="D77BF52921324D11" modified="2012-10-03" version="1">MVDGRKVLFARSSKSNNLSMGIVGLPNVGKSTLFNFLTRNNVPAENYPFCTIDPSEGRVEIQDERVEALAEKYMPHNVVRAYLSVTDIAGLVKGASTGVGLGNHFLDNIRNVDGIFHVVRCFEDPEVAHFEDGVDPIRDIEIVNEELRLKDYETLVRHEEKMSKDMKSKPSDKKLKDQVALVKRLVEILKNDWVNKHEFNEEEIEYISTLNLLTVKNVVYLANISEKDYEMRRANKHLRAVMKYSDDVIVFSAAYESNHESKVFLDKLVKKGYESLDLINFFTVGKDEVRSWTIRRGINAPSAGAVIHSDFKKYFVMAEVMSFEDFKAYGSEAEVKRAGKYHQRGKTYVVNDGDIILFKVNPPKSVGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU84</accession>
    <name>I6ZU84_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">High mobility group protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_061250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264713.1">
        <property type="nucleotide sequence ID" value="XM_009266438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU84"/>
    <dbReference type="GeneID" id="20521521"/>
    <dbReference type="KEGG" id="ero:EROM_061250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061250"/>
    <dbReference type="HOGENOM" id="CLU_124554_0_0_1"/>
    <dbReference type="OrthoDB" id="1919336at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050342">
        <property type="entry name" value="HMGB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48112">
        <property type="entry name" value="HIGH MOBILITY GROUP PROTEIN DSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="HMG box" evidence="4">
        <location>
            <begin position="116"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="2">
        <location>
            <begin position="116"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="181" mass="21110" checksum="A5D5F78DF91646FE" modified="2012-10-03" version="1">MVTEKNKKPRSKAMVKRRKDPNAPKKPMSGYFIFGQEQRKKNEELSKLPVAEQGRSISEMWKKLTDEEREEYNKISNKERELYQAKVEEYKKSAEYHDYLEKVAADEEAAGKKKKGVKKVTGYNEFFKVIRKAVSEENPNFTMMETTSAVAKRWKELSDDEKAVYNKIAEEKNMKAGLVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUJ1</accession>
    <name>I6ZUJ1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264853.1">
        <property type="nucleotide sequence ID" value="XM_009266578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUJ1"/>
    <dbReference type="GeneID" id="20521665"/>
    <dbReference type="KEGG" id="ero:EROM_071050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071050"/>
    <dbReference type="HOGENOM" id="CLU_1001252_0_0_1"/>
    <dbReference type="OrthoDB" id="2195775at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="280" mass="32343" checksum="FCEAF1B5EB7BF1BA" modified="2012-10-03" version="1">MEPSLILMFMSTFVNARDRELEEFIERDIKVFFSSYPAQVLGMEENNGVLVSHSKYVNPSKYKFITRARLVRSGDKYVVIFGENNICKDGNSVIKCKEERPWDIDRKEFGYTISTGNKCITKGPDETIEMKTCVNTDDQVFNFKLADVGGCGSIESLLGNGSPKNTTTNVNIFHPESEYLPSVKIKTRGNVNKNIILPTRKFESEDECSSHEEDIHSHVIEEMDRPLYEEVVPKRKVVVKRLPKKNRYHNHRVRRTHLERRHSPHHYGNRGFFGGEPIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUU4</accession>
    <name>I6ZUU4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal recognition particle Srp19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_080770</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264993.1">
        <property type="nucleotide sequence ID" value="XM_009266718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUU4"/>
    <dbReference type="GeneID" id="20521813"/>
    <dbReference type="KEGG" id="ero:EROM_080770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080770"/>
    <dbReference type="HOGENOM" id="CLU_154721_0_0_1"/>
    <dbReference type="OrthoDB" id="2190947at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005786">
        <property type="term" value="C:signal recognition particle, endoplasmic reticulum targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="137" mass="16024" checksum="A2CD105E20EB584E" modified="2012-10-03" version="1">METKYFCLYPIHMDSRRSLSEGRKYKKEICVQRPRYHEIKNALEKLEIEHVDEASKRHPNDFFNGGRFRIKKEYGKVLVIEGVSQTIAEMRSNSKGNKDLPKEKQSSGKVVQGVVQNGVYVENKLNLVRKKKVKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUU9</accession>
    <name>I6ZUU9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265003.1">
        <property type="nucleotide sequence ID" value="XM_009266728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUU9"/>
    <dbReference type="GeneID" id="20521823"/>
    <dbReference type="KEGG" id="ero:EROM_080880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080880"/>
    <dbReference type="HOGENOM" id="CLU_083362_0_0_1"/>
    <dbReference type="OrthoDB" id="2186465at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33242" checksum="0EC7CCFF958CC01E" modified="2012-10-03" version="1">MFPVNFAEIRLVKPDDFVFDKTICTEETNKPKILREYSKEEKACILKKRILAVAGRKGGWPQKKASRRESESMWDVDLSRADQGHFDQRGNFILGTPRSPSFSWLVKKEGSIYGPFTGREMKEKVNSEELKGTEIRRDIDKGFVSYDSLAIDLGDFLSSEKLDEYFEMHAAIKKPLEKSQEFFEDLSSLSLKRHPKKDLDRSKILEGCVKSRNFLHSRNPSVSLGFMERKISGKSFSDAIKIISNVAGFNKAECEEFLNLFLDESKLSILSDICPDGFVKVTSVTRKGSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV06</accession>
    <name>I6ZV06_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265078.1">
        <property type="nucleotide sequence ID" value="XM_009266803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV06"/>
    <dbReference type="GeneID" id="20521900"/>
    <dbReference type="KEGG" id="ero:EROM_081650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081650"/>
    <dbReference type="HOGENOM" id="CLU_863377_0_0_1"/>
    <dbReference type="OrthoDB" id="2193350at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="326" mass="36335" checksum="95368A5C9CE55465" modified="2012-10-03" version="1">MDIEKRISSVAATARRWLEDFRYIASIALNFLECFFYALAGIICLTYSEDPLKNIYCKYVSCILIALGIIDGLQKVPTTYLVKNSKAKEGTYKEKTIRYLKGLRKFFIKNKLSAVIDFFGALLMIFAISVMIISMCKVGGRVINSINATPNENGGRGLVTSVVFSSSGLYSLLSLLYSLYNLVMQGYQYISGFRARGVYSNTAGIVIRFENDEEEHHGVDKEPFSSMPMITLTEVIFMLIFIIWGTLKGGFGYVKQAFALSFIVQKIVTATLQYMAPFFRHSGSKNVILLPIIFISLLSLCAYVNGLGANIKGFQNLQNGQLVGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVB6</accession>
    <name>I6ZVB6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">T complex protein 1 subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_090490</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265163.1">
        <property type="nucleotide sequence ID" value="XM_009266888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVB6"/>
    <dbReference type="GeneID" id="20521988"/>
    <dbReference type="KEGG" id="ero:EROM_090490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090490"/>
    <dbReference type="HOGENOM" id="CLU_008891_6_2_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="466" mass="51483" checksum="452C05E57D2E72F3" modified="2012-10-03" version="1">MLKMLKGQNVNVTNDGAFILKNLMIDSPSARILIDSSTGQDWEEGDGTTSVAILASLLVKEASKLEIHPTRILKGYRMAQAKCEETLKSISFKPSKEDLIKLARTTLCSKVLKYDLEKFCDICISAIENLGGRNDLNLVQIIKCSGKLEDSYLDDGFLLKKDIRIEEMNDPKILIANTSMDQDKIKIFGAKINVSSVNELEEMERMEKAKIREKVERISQNGVNMFINRQLIYDHPLQLLKIKGIQAIEHADFDGVERLSNVLGGKILSTFDNIDESSYGTCKNVKNVYVGNERVIKFSGVRNGASTVVLCGSSKEILDEAERSIHDALCVLSRIKEDPRVVYGGGSSEMAMAVALNDYAMEVSGVESEAILAFSNALQQIPKILADNGGYNGEEIKASLRAKHNSEKVSHGVNVKSGGISCMKEAGVIDSFRIKHRVITAASEAAQMIIKCDAIVKCRPRERTRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVH2</accession>
    <name>I6ZVH2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091150</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265228.1">
        <property type="nucleotide sequence ID" value="XM_009266953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVH2"/>
    <dbReference type="GeneID" id="20564339"/>
    <dbReference type="KEGG" id="ero:EROM_091150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091150"/>
    <dbReference type="HOGENOM" id="CLU_2133497_0_0_1"/>
    <dbReference type="OrthoDB" id="2192531at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="96" mass="11054" checksum="59F924F5C22C3119" modified="2012-10-03" version="1">MKHMEDIKKARMREKHFRMKDKGYFLSLNMLGNNTDILSVFLGYIEGLGVDKRNVYSNIADAVLDGNNAIVENLRSVLKLEIRDEVSIVEEITKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVK1</accession>
    <name>I6ZVK1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265263.1">
        <property type="nucleotide sequence ID" value="XM_009266988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVK1"/>
    <dbReference type="GeneID" id="20564374"/>
    <dbReference type="KEGG" id="ero:EROM_091500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091500"/>
    <dbReference type="HOGENOM" id="CLU_1074009_0_0_1"/>
    <dbReference type="OrthoDB" id="2195087at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="95"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="172"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="288" mass="33309" checksum="35D35C919EBC8B47" modified="2012-10-03" version="1">MEGEEKISASRRKVNESGWDEYSILNDSGYEKRSVDKENSLEWKSFENEGDGISTRSQGNIDCGLSGEISSIKRHRCDVEANKEKNVSQALRVKESVTRAPEPNEIGREHSIAKEASRRNVSEYQEVVPNISRQRYEEHMRKADCVSKEYKPGDEVSGGGGERTQEIQIQKEENMRKAIKIQEEDIQAKNQEILLLKEKISRLESKLEIEGKEYVRGCVLRGEEAIRIVEEELKKERGDFLKKIGEEAEKTRMLLRRIDGLKKIINELVRKIREMKQKTEGSRREDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVM0</accession>
    <name>I6ZVM0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar import and degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265283.1">
        <property type="nucleotide sequence ID" value="XM_009267008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVM0"/>
    <dbReference type="GeneID" id="20564394"/>
    <dbReference type="KEGG" id="ero:EROM_091700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091700"/>
    <dbReference type="HOGENOM" id="CLU_007002_1_1_1"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="1">
        <location>
            <begin position="259"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="576" mass="66112" checksum="85C9598C9786337B" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW20</accession>
    <name>I6ZW20_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphomevalonate kinase</fullName>
            <ecNumber evidence="2">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_101410</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="2"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265453.1">
        <property type="nucleotide sequence ID" value="XM_009267178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW20"/>
    <dbReference type="GeneID" id="20564571"/>
    <dbReference type="KEGG" id="ero:EROM_101410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101410"/>
    <dbReference type="HOGENOM" id="CLU_789954_0_0_1"/>
    <dbReference type="OrthoDB" id="2193885at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="351" mass="40140" checksum="DFDCFD1A04E6BB84" modified="2012-10-03" version="1">MNERIQFKVPGKVIVNGSYIVLNGETCRAIALKTYILSETTRIVSDKPEITIEINGKEKSTYCYEDYESSIINGPDCYLLKIVDCFFRITGISPKNIINIKMKAEDGFFINGPTREKTGIGSSACILVSIVYALLKFHQEDLEKIILQDSFNGWGKSPRFQGDLRSWLGHLSLSEEIVEHVLPVTYLVHQKVNQEGSGSDAICCLLGSIYFNRKTCVPMEKIPRYLVLGSFGKSTSTKEMLKRIDLEDPRWESLKAINFEINRGRVCQKYLYMEYLNAMRDISIDIVPKKQYEILMKTSEYDIWGCGISGAGGDDCIWAISDDYKDVYRYWEKVFTFTFVTEVSYRGICLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW77</accession>
    <name>I6ZW77_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265513.1">
        <property type="nucleotide sequence ID" value="XM_009267238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW77"/>
    <dbReference type="GeneID" id="20564633"/>
    <dbReference type="KEGG" id="ero:EROM_110340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110340"/>
    <dbReference type="HOGENOM" id="CLU_506248_0_0_1"/>
    <dbReference type="OrthoDB" id="2190422at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="538" mass="63413" checksum="BE9A52EC7EF15904" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWA3</accession>
    <name>I6ZWA3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="6">3.1.13.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Carbon catabolite repressor protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cytoplasmic deadenylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Glucose-repressible alcohol dehydrogenase transcriptional effector</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">EROM_110640</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24 25">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCR4/nocturin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="6"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265543.1">
        <property type="nucleotide sequence ID" value="XM_009267268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWA3"/>
    <dbReference type="GeneID" id="20564663"/>
    <dbReference type="KEGG" id="ero:EROM_110640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110640"/>
    <dbReference type="HOGENOM" id="CLU_016428_4_2_1"/>
    <dbReference type="OrthoDB" id="428734at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF100">
        <property type="entry name" value="POLY(A)-SPECIFIC RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="16" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="23">
        <location>
            <begin position="33"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="22">
        <location>
            <begin position="175"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00030493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00033317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AFN84046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="493" mass="57057" checksum="F675692EFF499647" modified="2012-10-03" version="1">MGEECLAQRRKCFGKTSETHEEPWTGLDLCSQGIRNISKSLFDMRFIRVLNLSNNEIETIPKEICNLRCLEVLNLSKNKIRSIPPEIGKVVSLKELNLSDNLISNIPMEIGTLYNLESLEIANNPLIVPFNTLIRDKKLLQFCREHNTGYPPPNDRSWIERLNKNAFHNDVISVGTFNILSNLWAAKLTYAPSWVINPEFRREGILQEIVLYNVDILCLQEIELYSFFDFYKEQLEMRCSYDSIIYPRGKVRSVPDKKAVDGCAIFWRRNKFRLIAQFPIDFYQKVSQDTRFNTNQELLDRYGKKDNVAIGALLERPNGQQILVVNTHIFWDPDYPDIKLLQTVLLIEEIRKIISRHPNAYLFLQGDFNSLRSSSVYKSITTQTIDLTDFGNIAHHLSIQELGDGLKLNDSYLNQDLGFTNFTPVFKDVIDYIFYDSRMVLASVLSPVEDEYTENVAGLPNMHFPSDHIFLGAKFTFPNRNTTQNVFGRNLRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWC8</accession>
    <name>I6ZWC8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Poly(A)-binding protein-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_110890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265568.1">
        <property type="nucleotide sequence ID" value="XM_009267293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWC8"/>
    <dbReference type="GeneID" id="20564688"/>
    <dbReference type="KEGG" id="ero:EROM_110890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110890"/>
    <dbReference type="HOGENOM" id="CLU_765104_0_0_1"/>
    <dbReference type="OrthoDB" id="2275718at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045117">
        <property type="entry name" value="ATXN2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009604">
        <property type="entry name" value="LsmAD_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854">
        <property type="entry name" value="ATAXIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12854:SF7">
        <property type="entry name" value="ATAXIN-2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06741">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01272">
        <property type="entry name" value="LsmAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LsmAD" evidence="2">
        <location>
            <begin position="137"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="360" mass="40436" checksum="80F6168BEB1CFDB4" modified="2012-10-03" version="1">MDNYVISICFKNNPSKMIGTFLSNEDGMVKLEDAFFENNPGIVFPTISISESDIITVKKADARPEEEKERIPLEASEKTESGMKDSEQGLFKTDSEISEVPEEMRTKTVLVETKDITILPEEESKDWNQFEANSKLFNIDNKFDEEEYMDVLDKSSNWYKSKLLMARRIEKEIMSSTTTDPHRLEERGLGRTPENEDMYSTVIGKDHISVANKTPSSVEKQSKDASGIIEAKRGGDDGEKRKMIIEIEGLSLSETKDGGSLANTESSKNEESSNTQEERKNTRPKKSVRYGWMHTKFDSSKNLLGMIKSRFKGAFDISGSDLKWGGGLTWEIAAKNIIKKAKGGKASSIRRVPKKSPGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACT3</accession>
    <name>I7ACT3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263892.1">
        <property type="nucleotide sequence ID" value="XM_009265617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACT3"/>
    <dbReference type="GeneID" id="20520677"/>
    <dbReference type="KEGG" id="ero:EROM_010510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010510"/>
    <dbReference type="HOGENOM" id="CLU_072378_1_1_1"/>
    <dbReference type="OrthoDB" id="10257948at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21148">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="42"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="147" mass="17396" checksum="F82ED13EC10B698A" modified="2012-10-03" version="1">MPSGYEDSGFPQDDEAFMRYREKRIKELHGRIEDITAEKELIEKTKSLKMIVHFYKPEFNRCKIMDRRLEEIRDYFPGIQFYRVNAEMCPVVTKKLEIRVLPFLGFFKDGYFVDQVVGFEKLGDDVELEALKERILGSNIFKPISSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACY2</accession>
    <name>I7ACY2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_011060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263947.1">
        <property type="nucleotide sequence ID" value="XM_009265672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACY2"/>
    <dbReference type="GeneID" id="20520733"/>
    <dbReference type="KEGG" id="ero:EROM_011060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011060"/>
    <dbReference type="HOGENOM" id="CLU_475676_0_0_1"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="402"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="575" mass="66352" checksum="430DF73C9F234DE0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD57</accession>
    <name>I7AD57_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA replication factor C small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_020600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264032.1">
        <property type="nucleotide sequence ID" value="XM_009265757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD57"/>
    <dbReference type="GeneID" id="20520820"/>
    <dbReference type="KEGG" id="ero:EROM_020600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020600"/>
    <dbReference type="HOGENOM" id="CLU_042324_1_1_1"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="32"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="285" mass="32060" checksum="264555343319590F" modified="2012-10-03" version="1">MLWTEKYRPKSADMFEGPDHLKNILRKSDGRGHPNLLLYGPPGTGKTTFAHLLANRKLELNASDERGISVIRERIKVYASTLDKDKTIILDECENLTSDAQHCLRRVIEDSVNTRFIFITNYPSKIIGPLRSRLVSVKFSLMENGILEDIGKKEGLGYDKELYCRLFGLCGNDLRKAINVLQGIAPLSSFCIEEAVGAIPQKVIDAFWNANRPEVMNYAKEFLREGYSALQLVRQLSGSNKGSEVQSAEFHLVLSFLEEKSVSGCSDELILYNLLGRKVEIFNAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD89</accession>
    <name>I7AD89_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_020900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264062.1">
        <property type="nucleotide sequence ID" value="XM_009265787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD89"/>
    <dbReference type="GeneID" id="20520851"/>
    <dbReference type="KEGG" id="ero:EROM_020900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020900"/>
    <dbReference type="HOGENOM" id="CLU_1261500_0_0_1"/>
    <dbReference type="OrthoDB" id="2191456at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707771" evidence="3">
        <location>
            <begin position="19"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="96"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="221" mass="24794" checksum="339F42EED1804A8C" modified="2012-10-03" version="1">MRLLVVSMILGFRAFCKSEDSSAKSTDSIFKKFLEVAVKDEHKRLLPSKDRNHGMPHTQGPSHPAQRKKTVIVLEEDSEKKSMILNPKMGQKLESISDEVEKKNTASTSHKPLKTASEDTPKPEIQPDLTFETPISASFLLKPLEPKKEIALTQAEVKEIKEGLKALDKISKDLESLKARFQSVSKRILGSEKEEKGAKPPTDLLNYNFIEVTETGQPEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADA5</accession>
    <name>I7ADA5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_021050</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264077.1">
        <property type="nucleotide sequence ID" value="XM_009265802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADA5"/>
    <dbReference type="GeneID" id="20520867"/>
    <dbReference type="KEGG" id="ero:EROM_021050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021050"/>
    <dbReference type="HOGENOM" id="CLU_123486_0_0_1"/>
    <dbReference type="OrthoDB" id="29558at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="194" mass="22230" checksum="56F7B9ADBDA1EBE3" modified="2012-10-03" version="1">MQMIADQNPFGVSLTAVKASAKYLPYMDNLKAKYNKNPLRGDIYNLSELKIILDDVIRDFMEKVRNSRQSKASTDIKIVIGLISTAIASVVAYLSVTSEFEYHRRIIAVLTLLYFIINILGEVYFRYIGFSMVFEDRVVSTNISAPDPTYVILVYNKNKLIPSKYTKSVFDLFDSNGRLVHEEFLDDLQALFEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADQ7</accession>
    <name>I7ADQ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">14-3-3 protein 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030890</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 14-3-3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264207.1">
        <property type="nucleotide sequence ID" value="XM_009265932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADQ7"/>
    <dbReference type="GeneID" id="20521000"/>
    <dbReference type="KEGG" id="ero:EROM_030890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030890"/>
    <dbReference type="HOGENOM" id="CLU_058290_0_1_1"/>
    <dbReference type="OrthoDB" id="10260625at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="CDD" id="cd08774">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.190.20">
        <property type="entry name" value="14-3-3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000308">
        <property type="entry name" value="14-3-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036815">
        <property type="entry name" value="14-3-3_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023410">
        <property type="entry name" value="14-3-3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18860">
        <property type="entry name" value="14-3-3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00244">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000868">
        <property type="entry name" value="14-3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00305">
        <property type="entry name" value="1433ZETA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00101">
        <property type="entry name" value="14_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48445">
        <property type="entry name" value="14-3-3 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="14-3-3" evidence="3">
        <location>
            <begin position="7"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with phosphoserine on interacting protein" evidence="2">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000868-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="258" mass="29789" checksum="5F9E186C9E65CA51" modified="2012-10-03" version="1">MASKQYEEALQKANLSDMAERYDDMAKEMKHAVMLAHEEKHTLNVMARNLFSVAYKNLVSSRRSSWRMLCSERQKLEGKDPSVVHVIDEKIKMVEEELLRFCDEVLDIITTYILSLEDAKKNIEYNIFFLKMKGDYYRYKAEVITGSEHSEVSKHAAESYREATEKAKTLPPTNPIKLGLALNYSVFHYEILNDSEKACSIAKGAFDEAIKELDTLSEEHYRDSTLIMQLLRDNLTLWTSREEGSVIGEERKGDPDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADV2</accession>
    <name>I7ADV2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_031480</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264267.1">
        <property type="nucleotide sequence ID" value="XM_009265992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADV2"/>
    <dbReference type="GeneID" id="20521062"/>
    <dbReference type="KEGG" id="ero:EROM_031480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031480"/>
    <dbReference type="HOGENOM" id="CLU_049065_0_0_1"/>
    <dbReference type="OrthoDB" id="194358at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="275"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="450" mass="52021" checksum="7BE66FC2DC52F4D9" modified="2012-10-03" version="1">MKAEKPVIIRKKRQEYNEFIANDADWNDQRIALIGLYSTYQMYKDAGVAGDIVPDILAKPSFKMSRGTNIAGYKLHLAKLALIDGRPFKFNFEIRRHYFNQANIVFLLGMAKNNPEIVYSMLSKGFPNNINAPVFRSQMFPTYFHLACAMHHDILSVFLKFSPNYSLCWNGLTPQMIASFNGKSLEAKQPFNFVSMRQYRLLNKFRGFTLAEADEKPIFLIDFLCMKSDMESARKILNRNPELSRVSKICYLIQDDIEWIMFLSRYQTSVHQEFNGISPLHVSSISGNFETLVAFIALGSHINSRDKLGNTPMHYCAMLKHFRCLELLIRLGGNMTMVNREGVSTEDILLNLGKAFNIKEVDVELSEALFQILGDSVEFRHYLSIVDRIKYNSNHTIVKKNRFTITSLLNLPHKIDYTEDLIYKISIVRDDKKSNHTPRRTLQMILEHIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE77</accession>
    <name>I7AE77_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">EROM_050060</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264437.1">
        <property type="nucleotide sequence ID" value="XM_009266162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE77"/>
    <dbReference type="GeneID" id="20521239"/>
    <dbReference type="KEGG" id="ero:EROM_050060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050060"/>
    <dbReference type="HOGENOM" id="CLU_040729_6_0_1"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 19" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12 19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13 16 18" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 19" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
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        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
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        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AFN82940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="221" mass="25005" checksum="62D4DFD1B01415B4" modified="2012-10-03" version="1">MLDNIQEYLGVVKAKLAEFYEKVFQNFVKSLFGKPSSILFLGIDNAGKTTLVNKLKSDSTDVYMPTHHPSTSYIEIGNLKAQVIDLGGHTAARLAWRDYFYDCHGIVFIVDVHDVERFSEVREAYETVKSLEKKAPVVVLMNKIDLEGHTPETAEADYQWKSWLSQETGIEDQEDPERGQVVKIFYVTITSGSANSITGPLARAFKWLEAMISYNNKKESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE93</accession>
    <name>I7AE93_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RSC chromatin remodeling complex subunit RSC8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050320</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264462.1">
        <property type="nucleotide sequence ID" value="XM_009266187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE93"/>
    <dbReference type="GeneID" id="20521264"/>
    <dbReference type="KEGG" id="ero:EROM_050320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050320"/>
    <dbReference type="HOGENOM" id="CLU_055653_0_0_1"/>
    <dbReference type="OrthoDB" id="118550at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802:SF41">
        <property type="entry name" value="BRAHMA ASSOCIATED PROTEIN 155 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12802">
        <property type="entry name" value="SWI/SNF COMPLEX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SWIRM" evidence="6">
        <location>
            <begin position="47"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="215"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="7">
        <location>
            <begin position="218"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="400" mass="45922" checksum="731F8D0036D858E5" modified="2012-10-03" version="1">MENIFPLQENVEIEEIYGSKRPNASPPKNSRTFGMRSGAYGFIDTETYHVRFPNWFKKGEVSPLEQNGMSEVIQAIGPEKYVQSRDCIISSYEEGNRPMTVGKAFTLVDVDMGLMLKIFSFIERWRIINYRSLIEKEMRCLKSHKTEDAKPMETTPREERSKRRGDIKKCLEESICDCGGKANFFTRSLVFRCTACLDNGNYPQEVLRSDFLPITGSLIRNMWSRKEEFLLLEGIRKFGDEWDSVSRYVQTKTKEQCVFHFLRIPILENTLSRADLAIGRLFETAENPTMCIVTFICGMVHPKVASECARVAIKHISGCSQEAVIQHILDAGKEKAREQRDLERRKIERLGSVICEALLNKIKMKIGTYKELYLSTQRVRSELVALRRGLAEDINPGGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEI2</accession>
    <name>I7AEI2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase</fullName>
            <ecNumber evidence="4 14">4.2.1.134</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_051500</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a very-long-chain (3R)-3-hydroxyacyl-CoA = a very-long-chain (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="ChEBI" id="CHEBI:85440"/>
            <dbReference type="EC" id="4.2.1.134"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endoplasmic reticulum membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the very long-chain fatty acids dehydratase HACD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.134" evidence="4 14"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264577.1">
        <property type="nucleotide sequence ID" value="XM_009266302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEI2"/>
    <dbReference type="GeneID" id="20521382"/>
    <dbReference type="KEGG" id="ero:EROM_051500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051500"/>
    <dbReference type="HOGENOM" id="CLU_034302_6_1_1"/>
    <dbReference type="OrthoDB" id="46988at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102158">
        <property type="term" value="F:very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030497">
        <property type="term" value="P:fatty acid elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007482">
        <property type="entry name" value="Tyr_Pase-like_PTPLA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11035:SF3">
        <property type="entry name" value="VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04387">
        <property type="entry name" value="PTPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 14" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="192" mass="22972" checksum="6D670C2073D7B2E6" modified="2012-10-03" version="1">MMRLTYIRIYNAIGFIVCTIALFTSLMFYKTMKTKYLKMAGLCQTFFLMEVANICAKKSNARYLPTVMQLVSRMLIIWVVFWYYEIIDWTFPIMTACWYLSDLVRYVFYMFRIDTIRIIRYNLFLLTSPIGFILEMCCLRTLYNLIGNILSYLVALAALLYIPGFIFLFSHMLRQRKWSRKIKASKNKKKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEK5</accession>
    <name>I7AEK5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Vacuolar ATP synthase proteolipid</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_060100</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264602.1">
        <property type="nucleotide sequence ID" value="XM_009266327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEK5"/>
    <dbReference type="GeneID" id="20521407"/>
    <dbReference type="KEGG" id="ero:EROM_060100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060100"/>
    <dbReference type="HOGENOM" id="CLU_107724_0_0_1"/>
    <dbReference type="OrthoDB" id="10264021at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18178">
        <property type="entry name" value="ATP-synt_Vo_c_ATP6F_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
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                <person name="Whitman D.W."/>
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            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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    <accession>I7AFH2</accession>
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                <person name="Whitman D.W."/>
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            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264962.1">
        <property type="nucleotide sequence ID" value="XM_009266687.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFH2"/>
    <dbReference type="GeneID" id="20521779"/>
    <dbReference type="KEGG" id="ero:EROM_080430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080430"/>
    <dbReference type="HOGENOM" id="CLU_2146577_0_0_1"/>
    <dbReference type="OrthoDB" id="10052260at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="15253" checksum="6082B535C888B13E" modified="2012-10-03" version="1">MDSRMIVDMKKIIDDVETSSNQSQAWCMFDTNEDTCWFSGHGTQQSIDIYLKQTLYLQKIRIFFQRGFHCTKGKGYAASNHFSGYTRMEFHNGGSMGCLDINRDGDHIKIVLEEGADMYNRYCIYRISLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFT1</accession>
    <name>I7AFT1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_081850</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265097.1">
        <property type="nucleotide sequence ID" value="XM_009266822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFT1"/>
    <dbReference type="GeneID" id="20521920"/>
    <dbReference type="KEGG" id="ero:EROM_081850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081850"/>
    <dbReference type="HOGENOM" id="CLU_035750_7_3_1"/>
    <dbReference type="OrthoDB" id="37597at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03761">
        <property type="entry name" value="proteasome_beta_type_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF3">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 9 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="229" mass="25455" checksum="AF417A3E2DD417EF" modified="2012-10-03" version="1">MEETLKRDIRRIDTTCIDQKLVRSIIKPYHGTTTLAFVFKEGMVIAVDSRATSGSYIASQTVNKVIEINRNLLGTMAGGAADCFFWEKLMGLYAKNYELTNGRRITAAAASMYLSNCVYRYKGYGLSMGTMICGYDGSIPSIYYVDDDGKRVKGNLFSVGSGSTVAYGILSSSYRFDMSKQEALELGREAIFHAAHRDAYSGGSINLYYMDKDGWTKVGSYDVDDFNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGB4</accession>
    <name>I7AGB4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265297.1">
        <property type="nucleotide sequence ID" value="XM_009267022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGB4"/>
    <dbReference type="GeneID" id="20564409"/>
    <dbReference type="KEGG" id="ero:EROM_091850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091850"/>
    <dbReference type="HOGENOM" id="CLU_1210154_0_0_1"/>
    <dbReference type="OrthoDB" id="2190150at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036241">
        <property type="entry name" value="NSFL1C_SEP_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102848">
        <property type="entry name" value="NSFL1 (p97 ATPase) cofactor p47, SEP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="232" mass="26262" checksum="666094309241E4E7" modified="2012-10-03" version="1">MNLEARIKKIMEEARCSYDAAKKADQESHGNLDVAIETAKKKGNVLYSGGKSGLYVEENPQRKSITQYKNGILVEDKFYDFSVNDNIRLKNMLEKGTFDASLLGLHGDTAEVAYTEKLEEEYKEESKAHETDPKAAFIGEGRQLGNSNRDIPHTNIEDILEIARDGDVLFKVMIGSKRVKVKMLRSQTIGDFFNYMERYYDFGLVLSSNGKEIPPSHSVEEIANKLVLLSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGN4</accession>
    <name>I7AGN4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_101250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265437.1">
        <property type="nucleotide sequence ID" value="XM_009267162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGN4"/>
    <dbReference type="GeneID" id="20564555"/>
    <dbReference type="KEGG" id="ero:EROM_101250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101250"/>
    <dbReference type="HOGENOM" id="CLU_032061_0_0_1"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="71"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="336"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="4">
        <location>
            <begin position="385"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="549" mass="62123" checksum="91B618930583675F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGX7</accession>
    <name>I7AGX7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">HrpA-like helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_110730</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265552.1">
        <property type="nucleotide sequence ID" value="XM_009267277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGX7"/>
    <dbReference type="GeneID" id="20564672"/>
    <dbReference type="KEGG" id="ero:EROM_110730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110730"/>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001922">
        <property type="entry name" value="DEAH (Asp-Glu-Ala-His) box polypeptide 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="67"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="240"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="662" mass="75416" checksum="B505BACA2F85D1E9" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL96</accession>
    <name>I7AL96_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263926.1">
        <property type="nucleotide sequence ID" value="XM_009265651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL96"/>
    <dbReference type="GeneID" id="20520712"/>
    <dbReference type="KEGG" id="ero:EROM_010850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010850"/>
    <dbReference type="HOGENOM" id="CLU_481547_0_0_1"/>
    <dbReference type="OrthoDB" id="2195779at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="562" mass="64961" checksum="D06D2A9C119BD5B2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALC1</accession>
    <name>I7ALC1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">EROM_011150</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. COT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263956.1">
        <property type="nucleotide sequence ID" value="XM_009265681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALC1"/>
    <dbReference type="GeneID" id="20520742"/>
    <dbReference type="KEGG" id="ero:EROM_011150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011150"/>
    <dbReference type="HOGENOM" id="CLU_000288_140_4_1"/>
    <dbReference type="OrthoDB" id="3638488at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00029">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000751">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.160">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036274">
        <property type="entry name" value="HR1_rpt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF71">
        <property type="entry name" value="CITRON RHO-INTERACTING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00742">
        <property type="entry name" value="Hr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46585">
        <property type="entry name" value="HR1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="45"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="18">
        <location>
            <begin position="299"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="17">
        <location>
            <begin position="781"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="399"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="580"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="736"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AFN82459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="863" mass="99311" checksum="0155CCD660BFE9C2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALL4</accession>
    <name>I7ALL4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Archease domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_020890</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264061.1">
        <property type="nucleotide sequence ID" value="XM_009265786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALL4"/>
    <dbReference type="GeneID" id="20520850"/>
    <dbReference type="KEGG" id="ero:EROM_020890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020890"/>
    <dbReference type="HOGENOM" id="CLU_1740510_0_0_1"/>
    <dbReference type="OrthoDB" id="2190767at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="5">
        <location>
            <begin position="21"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="150" mass="16359" checksum="CF0486BCC1BB0C3E" modified="2012-10-03" version="1">MQSVDHRAAKNPKAEGMKSVEFLDHLADVQMHCTAPCLPLLYETAVKGMMSYAVKAPTVAKGGGRVELSEGSNEMNMVALLTHFIDLMYGEGFVAIEVSVALKNGLLVCDYSTADGSKCQSLCEIKAITLCDLRIFEKDGVFHSYCIFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALX1</accession>
    <name>I7ALX1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphoglucomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_030240</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264141.1">
        <property type="nucleotide sequence ID" value="XM_009265866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALX1"/>
    <dbReference type="GeneID" id="20520933"/>
    <dbReference type="KEGG" id="ero:EROM_030240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030240"/>
    <dbReference type="HOGENOM" id="CLU_486617_0_0_1"/>
    <dbReference type="OrthoDB" id="8300170at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="64"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="263"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="362"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="564" mass="63534" checksum="F04F54335563CE12" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM96</accession>
    <name>I7AM96_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_040460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264311.1">
        <property type="nucleotide sequence ID" value="XM_009266036.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM96"/>
    <dbReference type="GeneID" id="20521110"/>
    <dbReference type="KEGG" id="ero:EROM_040460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040460"/>
    <dbReference type="HOGENOM" id="CLU_002173_5_2_1"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF8">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="423"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="744" mass="86452" checksum="64058114D1C53C8E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AME4</accession>
    <name>I7AME4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264371.1">
        <property type="nucleotide sequence ID" value="XM_009266096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AME4"/>
    <dbReference type="GeneID" id="20521171"/>
    <dbReference type="KEGG" id="ero:EROM_041070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041070"/>
    <dbReference type="HOGENOM" id="CLU_070108_0_0_1"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="341" mass="37308" checksum="11222AD2E981267F" modified="2012-10-03" version="1">MYVVGDVTTGVVDIGSETVKIGYSDGLLPTVFCSSMTWKNEYVPPVTRSVVSDVGSYLRILSDHVPGDAQSLVISENTMEAGGVKKEILGYLMEGRLCESVLFVRSGILDAFSYGKTTGVVVNLGGGSSQICSVVDGLITCRRQIDVGGMDLTLEFMRILESFGSDLGKFIDVKNGETLYERRVEFEKKEFSRHVKESVLRLRSEEANGRYVLPDGKEIDAEEYCKAMEIKLGGVIKMIVEVIESNGMEIRSVLSGNILVGGGCGGIGGIDSFICDEIGKERGRWKVKVSVERSRFSTFQGGCVIGSMGSARSFHIGVRDYEEYGESILDRKKCEWIVEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMH1</accession>
    <name>I7AMH1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rad21/Rec8-like protein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_041380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264401.1">
        <property type="nucleotide sequence ID" value="XM_009266126.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMH1"/>
    <dbReference type="GeneID" id="20521201"/>
    <dbReference type="KEGG" id="ero:EROM_041380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041380"/>
    <dbReference type="HOGENOM" id="CLU_821425_0_0_1"/>
    <dbReference type="OrthoDB" id="2194777at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008278">
        <property type="term" value="C:cohesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="3">
        <location>
            <begin position="23"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="338" mass="38360" checksum="3F898880A0ABEBC1" modified="2012-10-03" version="1">MPGAIGILGLVNTQDALFKAIFMERRLSKHNLKEVSISAIVLKVMDESVSVKDGGLVVLGLTRIFVRKLKYLQDECSDTVHKIYQKGGDRGRGLKQPSSKGITLGLDLESLYIDDQMISPEEFVILPEEDNDCDAHEGVDFSFNEFNDISHVEEGRFEGEDNTRVSTTTEVTQIPLGERREKRRKIIEDSELEFDAQVFRENLRNTRDIVCKEKALDIRDELASKLSIAPEILSRICHREIHPRESVEGARDLTLLESYGDISFGDQVVESQASEEITIEESLDVEKLPDRFIFNELTIGHSRHEKGRMFLSLLKLLGDGVLQAYQDRSYSNIECRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMH5</accession>
    <name>I7AMH5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041450</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264406.1">
        <property type="nucleotide sequence ID" value="XM_009266131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMH5"/>
    <dbReference type="GeneID" id="20521208"/>
    <dbReference type="KEGG" id="ero:EROM_041450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041450"/>
    <dbReference type="HOGENOM" id="CLU_804174_0_0_1"/>
    <dbReference type="OrthoDB" id="2189728at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="346" mass="39745" checksum="AC90B46E331F09F9" modified="2012-10-03" version="1">MEEYTLCSSSTEQGEIPVIHVKRFKEEPPLFPRTRSQLQYMYLPNKPLFVGPNTIPRSCDYQANVLTTKLKKRLKHDLPYPFLCLSMNSDKTFFEDKFALVSKVHEVENVVSDLFLTKTKERIIHIKDEVGTGPEGRNEIKLEHIKDKQNDTYKDACQDFLAGKLFEDGEARRVNIIIKDSTINSLLKKDAYIHFYVKKKDGKLYAVFDEVYTREIKYYDYLSRYGEEVIRSLKSSEIRVYSSEEVTVAVYSSTLFYSSSGIQRLFYGIDVEKSIDVVVNIEDVRAVRSSGALGDEMKVIFGIGVEEGEYVLNDGRIYAVSPNGEKFVFDGVVFTDFLDNQESTSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMW9</accession>
    <name>I7AMW9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_051640</name>
        <name evidence="2" type="ordered locus">EROM_110020</name>
    </gene>
    <organism evidence="1 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264591.1">
        <property type="nucleotide sequence ID" value="XM_009266316.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265484.1">
        <property type="nucleotide sequence ID" value="XM_009267209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMW9"/>
    <dbReference type="GeneID" id="20521396"/>
    <dbReference type="GeneID" id="20564602"/>
    <dbReference type="KEGG" id="ero:EROM_051640"/>
    <dbReference type="KEGG" id="ero:EROM_110020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110020"/>
    <dbReference type="HOGENOM" id="CLU_1555240_0_0_1"/>
    <dbReference type="OrthoDB" id="2199999at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="172" mass="19498" checksum="FFB5A67B32E83C8F" modified="2012-10-03" version="1">MLAISVIGIIGLLGGLRGAIPLKVSNVSGKVYKNIRVNAQRICRIMEYVEKPEIAEFMKECLEKQVSERAQELCRELIFEHESLKEKVARLGEAAGNSESSFEELKSLIIEVFDGCFKVVRSGMILDIELGFNEENLDEVEEACSKVYRRLLNEIAQWRNSNGAFRLATTYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN64</accession>
    <name>I7AN64_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Snf2/Rad54-like helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_060760</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264666.1">
        <property type="nucleotide sequence ID" value="XM_009266391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN64"/>
    <dbReference type="GeneID" id="20521472"/>
    <dbReference type="KEGG" id="ero:EROM_060760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060760"/>
    <dbReference type="HOGENOM" id="CLU_000315_33_4_1"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18010">
        <property type="entry name" value="DEXHc_HARP_SMARCAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF6">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="135"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="385"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="559" mass="63828" checksum="60884C893957112E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANP0</accession>
    <name>I7ANP0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264896.1">
        <property type="nucleotide sequence ID" value="XM_009266621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANP0"/>
    <dbReference type="GeneID" id="20521712"/>
    <dbReference type="KEGG" id="ero:EROM_071480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071480"/>
    <dbReference type="HOGENOM" id="CLU_1510589_0_0_1"/>
    <dbReference type="OrthoDB" id="2193130at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="178" mass="20535" checksum="C1A1704A57A88EE6" modified="2012-10-03" version="1">MGYRTFCSFLQNLDLIRDKLKNRPGIRKIQNVLAYDGYHLTDYWGRLKFRKEGDKLVDETGYFEGIKVNSFGDMLTQGRMTRTGFVQKKDIGENEVNENLITMKLLLSEIAKEKNTSIHNVINEGCNMSMVEELVFYGNSPSAHNNEVMHVCVCSDSFCKEPCKDIVPMKVEDIEFRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7APC9</accession>
    <name>I7APC9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">40S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_090620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265176.1">
        <property type="nucleotide sequence ID" value="XM_009266901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APC9"/>
    <dbReference type="GeneID" id="20564286"/>
    <dbReference type="KEGG" id="ero:EROM_090620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090620"/>
    <dbReference type="HOGENOM" id="CLU_058591_2_1_1"/>
    <dbReference type="OrthoDB" id="10248446at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02413">
        <property type="entry name" value="KH-II_40S_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="8">
        <location>
            <begin position="19"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Small ribosomal subunit protein uS3 C-terminal" evidence="7">
        <location>
            <begin position="104"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="216" mass="24272" checksum="9EC262F6C22A897E" modified="2012-10-03" version="1">MSDDQVLKRFMKNGLINAELKEFFEKVLVNEGFSTMELRMQETPIKIILKVAKPHEAIGEKKLRLKQFQHLVAQRLEVPDENVEIIVEKVHEKGLCALIQANFIREKILGGVQYRRAVNMALKATRHSGAQGCQIIVSGKLKGQRAKSVKFQDGVLIHSGNAVKDYINTGYATVETKQGVIGIQVRIMLPYDPEGVIGPNYPLPDRITILEPTETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7APG8</accession>
    <name>I7APG8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265226.1">
        <property type="nucleotide sequence ID" value="XM_009266951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APG8"/>
    <dbReference type="GeneID" id="20564337"/>
    <dbReference type="KEGG" id="ero:EROM_091130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091130"/>
    <dbReference type="HOGENOM" id="CLU_029375_2_0_1"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="265" mass="30154" checksum="0D5963A835CB3880" modified="2012-10-03" version="1">MVLGSIAIQFIHVVLAAMILSLDFLFLLFTIQRSLYFFGKSMKRRPESMRVRLMSGCRSELYLIDCMSCTDLIYLPGNFVAIEEHIEFCKYLSKMLECNVISMVYRGIAGNCCSPSERGIIEDLSPISQWISRRSTRKVVLGFSIGSAVGVRLAEKCHVDALVLVNPFISLREVVSNILFGRVLKHFIVDEWNNVSRMKEINAPVYFVVSSNDEIVPPSHTDELIKKTRLPRMIVIPGADHNEPMRNFATHLYPVIGEILKEKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7APW3</accession>
    <name>I7APW3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265416.1">
        <property type="nucleotide sequence ID" value="XM_009267141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APW3"/>
    <dbReference type="GeneID" id="20564534"/>
    <dbReference type="KEGG" id="ero:EROM_101040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101040"/>
    <dbReference type="HOGENOM" id="CLU_330960_0_0_1"/>
    <dbReference type="OrthoDB" id="2191078at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="886" mass="102087" checksum="BA71A15CF0552E6D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ04</accession>
    <name>I7AQ04_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263889.1">
        <property type="nucleotide sequence ID" value="XM_009265614.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ04"/>
    <dbReference type="GeneID" id="20520674"/>
    <dbReference type="KEGG" id="ero:EROM_010480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010480"/>
    <dbReference type="HOGENOM" id="CLU_1768033_0_0_1"/>
    <dbReference type="OrthoDB" id="2190197at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="141" mass="16639" checksum="9A8BB2AD8408FA17" modified="2012-10-03" version="1">MFDSIDDIRLSDILFGSDIDASHCSKVSKKKEEEKSMWKKNVRHYKILSVDQISALEDFFISKQLPITKTNIKHASKALKIPYQRSVNYLYNKYKKAQENAEDFYHRCIHEFGLIMQNVEKCWEMYLKGCQAYNSTPDSRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQE3</accession>
    <name>I7AQE3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cyclin-dependent protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_111180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265596.1">
        <property type="nucleotide sequence ID" value="XM_009267321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQE3"/>
    <dbReference type="GeneID" id="20564717"/>
    <dbReference type="KEGG" id="ero:EROM_111180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111180"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF107">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 11A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="15"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="320" mass="36802" checksum="876F3FF1E136F6A8" modified="2012-10-03" version="1">MLLRGGHKCSNIEGYEKLCRISSGSFGNVYKVRRTADSKVFALKRMNPSMCYDTNGFSILYIREVMILKHARNKNVMKIEEVVEGNEINDFFIVMECCDIDLKSVIHRVREMDVRLIRFFRWQILQGLKFLHGIGVIHRDLKPSNILLMRNGDVKIADFGLARVMESTMTNLVVTLWYRAIEVLLGSESYNESIDMWSVGCIVGEMLKGEPILPGEGEIDQLGRIFKLLGYPEDSDFDGFDLPHFKNIKKPKSFSTSFEKEFGSYDSEVVAFTRNLLSFDPRRRYSASQALSSRFMTDGQECPRELIEEVMGMCIERFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQG2</accession>
    <name>I7AQG2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_020770</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="5"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264049.1">
        <property type="nucleotide sequence ID" value="XM_009265774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQG2"/>
    <dbReference type="GeneID" id="20520837"/>
    <dbReference type="KEGG" id="ero:EROM_020770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020770"/>
    <dbReference type="HOGENOM" id="CLU_015846_0_1_1"/>
    <dbReference type="OrthoDB" id="3168162at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF2">
        <property type="entry name" value="SERINE PALMITOYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="98"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="422" mass="47755" checksum="8E85AAEC9FD132AD" modified="2012-10-03" version="1">MEPIFTIGGLIVSYWKDILKIIIETFVIYLVVKFRYRSRKDIIIFPQSVIDKLVEEFDPDDLVKNVPSDVMLPSIYREGMIDLANFDVFGLSMENKEEIIEVIRKYGVGTCGPRGFYGTLDIHLDLEETISKELGAEASIVYPNSFTAVNSIIACFCKQQDIVFYHEDSNEAILRGIGLSKSKNIEFGSVSDLKIKLEYFTKPSVRNFVIVEGLSRNTGRIVDIRMILKLRDKYNFRIILDESYSIPLLNKRGVCGMNGISLREIDIVIGSLSGGLCSTGAFSTGTYYTVDYQKLSGSSYCFSASMPGGMAKAGILNIKRDFEHEELRDKVKAFHTSFRSKTYEIISHLLSPIIIVVKKKEIRRSMSKEALLKEVLDIRSELLENGIIIGFNHNPCPSLRACLKTDILEEDIARLVTLLNKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQM3</accession>
    <name>I7AQM3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265651.1">
        <property type="nucleotide sequence ID" value="XM_009267376.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQM3"/>
    <dbReference type="GeneID" id="20564772"/>
    <dbReference type="KEGG" id="ero:EROM_111730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111730"/>
    <dbReference type="HOGENOM" id="CLU_1199814_0_0_1"/>
    <dbReference type="OrthoDB" id="2189356at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707572" evidence="2">
        <location>
            <begin position="17"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="231" mass="26094" checksum="A29FC8FABABFEE22" modified="2012-10-03" version="1">MLFNLFLVFGSVIAIAEYYNHSIIPYIDRTGNGDAYSDYNAESSMISITESSALEGRTGNFTAEAIGDGKSERLDLSSLASSKADSPAGAIKSLRIGKFKINPENISDLIHREMADMSPDLHNKIVNTLERNSVDRRRYRDLFLIKKRVIDDGRRKIINFNIKEKIIPKEAEEDLPITRDFGTRALLGGIMFLLAGLVFTTTLKMYQSLIRKNYFSFDEKGEMFTETKQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQW1</accession>
    <name>I7AQW1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bis (5'-nucleosyl) tetraphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264194.1">
        <property type="nucleotide sequence ID" value="XM_009265919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQW1"/>
    <dbReference type="GeneID" id="20520987"/>
    <dbReference type="KEGG" id="ero:EROM_030760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030760"/>
    <dbReference type="HOGENOM" id="CLU_1057794_0_0_1"/>
    <dbReference type="OrthoDB" id="2190805at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="263" mass="30132" checksum="D37085F425EED7B9" modified="2012-10-03" version="1">MEESSVIGKIEEVFEIEDPSILPERVRSVIDEILEGGVFPTSHPFYVDFINLAFDVLYPDIQQPAELLRRSGFTRDELEKTGLKRLADMACSMSPKDAESRSELDEDSKPKRRKKVSWGANLIQVKEIERTVMDPEIDYLISDSGNNKVPEKTPDAFEWIIPEKLDHEYSVTKSPGSLEQERRESVSIRMCNTEEHRKFSPTQCMNVGEDNETITVPVISFVKSRVPDFDFKKIVEDRFNNMMPINEILEDSSVVNALLGKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR31</accession>
    <name>I7AR31_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Symplekin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264299.1">
        <property type="nucleotide sequence ID" value="XM_009266024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR31"/>
    <dbReference type="GeneID" id="20521097"/>
    <dbReference type="KEGG" id="ero:EROM_040330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040330"/>
    <dbReference type="HOGENOM" id="CLU_688929_0_0_1"/>
    <dbReference type="OrthoDB" id="2190938at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="401" mass="46969" checksum="DE1224B739603468" modified="2012-10-03" version="1">MTYKVDKKAIRRLYLMKNSGNPDICSSLSRLVEIGNPYLTFILQAMFQNMLSETSCPAPFAILMRSSKIVNYIVRRIIGKDIILEARDGPRKCDDSKWDENDYVEVMKFLLNLEKANRRISYIDNPFILYVVSKISEVEKARLIRFLEISPLCILIMKTMNTNSLSGIHLEVINFLKVKDMTYEEGFMYIHESCADFKALKREFLKSRFPQIQRYFHVLMDFYPEMMFGARKPYANRMKIFGDPLSIPIKPRLLCVYISACVYFIRRKYEALGQEKNLDVLMKAIYIERILSTCPKRRLLKEVIHQLILDTPILVKVIVMRRFPCNLVRKMVECVPSFHLAYELSLKILCKNPNDSFYEALVEELLKKYPTESNVRKFGACAHLFGKPLLERLRYLTDACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARG3</accession>
    <name>I7ARG3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="5 13">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_050290</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="5 13"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264459.1">
        <property type="nucleotide sequence ID" value="XM_009266184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARG3"/>
    <dbReference type="GeneID" id="20521261"/>
    <dbReference type="KEGG" id="ero:EROM_050290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050290"/>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_1"/>
    <dbReference type="OrthoDB" id="275353at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000031">
        <property type="entry name" value="Phosphoglycerate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 13 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="388" mass="43170" checksum="653B6E37C932E1B1" modified="2012-10-03" version="1">MKTIDEVSIEGKSVFLRVDFNVPISNLVVLNDFKIASVLPTIKFLHSKGPKKVIIGSHLGRPKGKYSKEFSLIPVYSVLKKLLGKEIGVELAFCDIWDVENTHHTWILLENLRFCSAEEREDDRDLVERYRNAFIKNMDIAVIDAFGCLHRECGSIQRTGLPSYSGFLVQKEIDSIREIAEENIDLMILGGKKISDKIKLLKSLGQRTKNLFVTGGLAFPFIKYILGKEVGDSVCEEVSKEEVKKIHEICESHGTRMLFPVDFTVLHNGRVQKSRTIPSEGTAMDIGPETIEMLKKEVSRANVAFWNGPPGVFEEKNFSDGTRALVNILESLKKRGGKSFCGGGETAGAIKLFGNYDGFTYVSTGGGVLMKLLSNEKLPGLDFLSNQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARV3</accession>
    <name>I7ARV3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">E2F/DP transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_060170</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the E2F/DP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264609.1">
        <property type="nucleotide sequence ID" value="XM_009266334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARV3"/>
    <dbReference type="GeneID" id="20521414"/>
    <dbReference type="KEGG" id="ero:EROM_060170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060170"/>
    <dbReference type="HOGENOM" id="CLU_095032_0_0_1"/>
    <dbReference type="OrthoDB" id="552115at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14458">
        <property type="entry name" value="DP_DD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.80">
        <property type="entry name" value="Transcription factor DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037241">
        <property type="entry name" value="E2F-DP_heterodim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003316">
        <property type="entry name" value="E2F_WHTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038168">
        <property type="entry name" value="TF_DP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014889">
        <property type="entry name" value="Transc_factor_DP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015648">
        <property type="entry name" value="Transcrpt_fac_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12548:SF9">
        <property type="entry name" value="TRANSCRIPTION FACTOR DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08781">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02319">
        <property type="entry name" value="WHD_E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01138">
        <property type="entry name" value="DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01372">
        <property type="entry name" value="E2F_TDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144074">
        <property type="entry name" value="E2F-DP heterodimerization region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="E2F/DP family winged-helix DNA-binding" evidence="9">
        <location>
            <begin position="12"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor DP C-terminal" evidence="8">
        <location>
            <begin position="79"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="211" mass="25040" checksum="4E164AD6D3181525" modified="2012-10-03" version="1">MKLNSEIDGSENKREGLRYITQAVFQLLRENRACTYSFICKNIVFPNTETLNRRIYDVLNVMKAVGLISKRGKRYFLVNDASDSDRRKEEAKKLMEMKKVFKFIVDRNSAAEDTHLERLYLPFMVVSTSKKADIHCETNEERNFFVFKSTKPLKVNEDLDILREIYEKAHSKEKTTDFSIVDRLSNERFEYFEENNYQREGLDPDAFFFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS25</accession>
    <name>I7AS25_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">60S ribosomal protein L5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_060840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264674.1">
        <property type="nucleotide sequence ID" value="XM_009266399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS25"/>
    <dbReference type="GeneID" id="20521480"/>
    <dbReference type="KEGG" id="ero:EROM_060840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060840"/>
    <dbReference type="HOGENOM" id="CLU_056222_1_0_1"/>
    <dbReference type="OrthoDB" id="1618453at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 9" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="8">
        <location>
            <begin position="232"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="287" mass="32911" checksum="497D59CE2EC26380" modified="2012-10-03" version="1">MTNAKGTRNYHKNFQVKRRRRREGKTDYKHRTNMIRQDSNSYGSVKHRLVVRVTNSKVICQIVGAYMEGDRVMVYADSSELPKYGVNFGLTNYSAAYATGFLIGRRALTALSLDKIYGPKEIDGSHSITKDVDGEKKAFRVFLDVGLARSTKGAKVFGAMKGASDAGLSIPHREVKFYGYKEDGSFNAQELHDRIFGYNISEYMQELRENNPEKYKIQFSDYIKKGIQPEDIPQIYKNAFDKIAEDPIKEKKEIKDYSGFKKYKQARLTYEERKERVQAKLSAAEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASC6</accession>
    <name>I7ASC6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">E3 ubiquitin ligase subunit cullin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_070810</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264829.1">
        <property type="nucleotide sequence ID" value="XM_009266554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASC6"/>
    <dbReference type="GeneID" id="20521640"/>
    <dbReference type="KEGG" id="ero:EROM_070810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070810"/>
    <dbReference type="HOGENOM" id="CLU_374286_0_0_1"/>
    <dbReference type="OrthoDB" id="5581181at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin family profile" evidence="4">
        <location>
            <begin position="462"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="742" mass="86843" checksum="CB523240C4E3FBF6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASG3</accession>
    <name>I7ASG3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mrk1-like Ser/Thr protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_071210</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264869.1">
        <property type="nucleotide sequence ID" value="XM_009266594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASG3"/>
    <dbReference type="GeneID" id="20521681"/>
    <dbReference type="KEGG" id="ero:EROM_071210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071210"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_20_1"/>
    <dbReference type="OrthoDB" id="272141at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050591">
        <property type="entry name" value="GSK-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057">
        <property type="entry name" value="GLYCOGEN SYNTHASE KINASE-3 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24057:SF0">
        <property type="entry name" value="PROTEIN KINASE SHAGGY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="40"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="318" mass="36881" checksum="25062D0DB7AE0D43" modified="2012-10-03" version="1">MVTLSQKKLVESIKLIEREWIKMHRALARSKCGEMKEVQYKYVEMIGKGSFGVVVKIMDDRCNFFALKRVYQDRRYHNRELGILMEIDHPNIVKLISYFHTDKTPSGMYLNIITDFAGINLEEYIKSNRSVEVRTIRSIYKQILEGLRYLHEKGICHRDIKPSNILIGPNGLVKICDLGSAKVIVRGERNITYICSRFYRAPENLLDYKEYDFKIDVWSAGCVIAEFRHPGPMFKGGTSGSTLNKILEIVRTSLEDLIELGYQKSDYKPGLGVRKYLEKFFDDTDLLDVLERSLTFSPCKRSTASELLSKPFFQCLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASN6</accession>
    <name>I7ASN6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cleavage stimulation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080300</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264949.1">
        <property type="nucleotide sequence ID" value="XM_009266674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASN6"/>
    <dbReference type="GeneID" id="20521766"/>
    <dbReference type="KEGG" id="ero:EROM_080300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080300"/>
    <dbReference type="HOGENOM" id="CLU_028601_2_1_1"/>
    <dbReference type="OrthoDB" id="272703at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025742">
        <property type="entry name" value="CSTF2_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45735:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14327">
        <property type="entry name" value="CSTF2_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="5"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="214" mass="24383" checksum="6E5A8A6D0ABBFD13" modified="2012-10-03" version="1">MKSGCSIFVGNIDFEVPEERIIEELGAVGRVVSFRMVYDKTTGKSRGFGFCEYESPLIVEKALQNLKISFNGRPVKINYAENDMPAKTKEPDSQIPEIDNLINVLDGMDKDNLKEVLFYLRKLALDQPSYLRELLNKNSSLVYAIIHAILKLKLADPGVVDEILKESFDLNKQKAQILERICTISEDDLCFFSEDVRSKIKKVRSMVLKSRVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT88</accession>
    <name>I7AT88_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265129.1">
        <property type="nucleotide sequence ID" value="XM_009266854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT88"/>
    <dbReference type="GeneID" id="20521953"/>
    <dbReference type="KEGG" id="ero:EROM_090140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090140"/>
    <dbReference type="HOGENOM" id="CLU_339192_0_0_1"/>
    <dbReference type="OrthoDB" id="2192860at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="786"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="795"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="827"/>
            <end position="840"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="851" mass="96735" checksum="578529B4DF3324E4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATI4</accession>
    <name>I7ATI4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265264.1">
        <property type="nucleotide sequence ID" value="XM_009266989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATI4"/>
    <dbReference type="GeneID" id="20564375"/>
    <dbReference type="KEGG" id="ero:EROM_091510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091510"/>
    <dbReference type="HOGENOM" id="CLU_1540530_0_0_1"/>
    <dbReference type="OrthoDB" id="2189693at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="174" mass="20044" checksum="035A83ED934E4082" modified="2012-10-03" version="1">MFDSEYFKSLLNETTALIANKVDEGSNGEEMEARIESNITTLKYLTRKITDEELRSKTLKRIDMLCAEPEVFEMNEVRNRAPGIRKSSEFSDRIERDILKYTKRLHGKVKEFMESVDVDSKVLGEVTDKMSRNLMGTSETLRSLKLKGRNIPALGILTSVMATFIVMYFVIRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATJ4</accession>
    <name>I7ATJ4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2428 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265279.1">
        <property type="nucleotide sequence ID" value="XM_009267004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATJ4"/>
    <dbReference type="GeneID" id="20564390"/>
    <dbReference type="KEGG" id="ero:EROM_091660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091660"/>
    <dbReference type="HOGENOM" id="CLU_332333_0_0_1"/>
    <dbReference type="OrthoDB" id="73997at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019442">
        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
        <property type="entry name" value="THADA/DEATH RECEPTOR INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2428" evidence="2">
        <location>
            <begin position="514"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="861" mass="99947" checksum="06F1ECC7EC134D53" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU49</accession>
    <name>I7AU49_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane adaptor Erv26-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_110240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVP26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265504.1">
        <property type="nucleotide sequence ID" value="XM_009267229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU49"/>
    <dbReference type="GeneID" id="20564623"/>
    <dbReference type="KEGG" id="ero:EROM_110240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110240"/>
    <dbReference type="HOGENOM" id="CLU_128914_0_0_1"/>
    <dbReference type="OrthoDB" id="28257at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097020">
        <property type="term" value="F:COPII receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007277">
        <property type="entry name" value="Svp26/Tex261"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144:SF0">
        <property type="entry name" value="PROTEIN TEX261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13144">
        <property type="entry name" value="TEX261 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04148">
        <property type="entry name" value="Erv26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="174" mass="19528" checksum="FC901F7118181528" modified="2012-10-03" version="1">MLSIFLKSLLLIVGSIGGLVGLGIGIVKAIDYIEDKAYAAKKHIESIIQISAGLHIILLLRGVSIFQILFSLSVQYAFYCLLDIYPLVKMDNPYFIYGTIASLLNHFLVIRLLFSKPGIYGAEIVLYFALVWTTPFCFFLSLSANDEVLFVRGKASPNTMIGNFLKKYLYKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU74</accession>
    <name>I7AU74_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">U6 snRNA-associated Sm-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265529.1">
        <property type="nucleotide sequence ID" value="XM_009267254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU74"/>
    <dbReference type="GeneID" id="20564649"/>
    <dbReference type="KEGG" id="ero:EROM_110500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110500"/>
    <dbReference type="HOGENOM" id="CLU_197704_0_0_1"/>
    <dbReference type="OrthoDB" id="422364at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="78" mass="8657" checksum="018CF49809CE10C6" modified="2012-10-03" version="1">MNKDKIKKTQLLSDLGRFKGKKITITFIDGKSVTGKLTSFDDVGNCVLKKCGEWDYGNTVVCLGRSIVLICLNTPYIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU94</accession>
    <name>I7AU94_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_110700</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265549.1">
        <property type="nucleotide sequence ID" value="XM_009267274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU94"/>
    <dbReference type="GeneID" id="20564669"/>
    <dbReference type="KEGG" id="ero:EROM_110700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110700"/>
    <dbReference type="HOGENOM" id="CLU_064104_4_2_1"/>
    <dbReference type="OrthoDB" id="154356at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07982">
        <property type="entry name" value="HFD_TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003923">
        <property type="entry name" value="TAF10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21242:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03540">
        <property type="entry name" value="TAF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01443">
        <property type="entry name" value="TFIID30KDSUB"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="108" mass="12514" checksum="1EE54A408FF80E80" modified="2012-10-03" version="1">MKEEEFNELKQNLDNYTPLLPESVTDYFMEKAGVATSDQSVKKLVSLLAHKFVTDIAVSSFQYHRINQKAAQKDKRFAKEKKPTFQLIDLEKALEEVGVSISRPHYYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUB9</accession>
    <name>I7AUB9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265574.1">
        <property type="nucleotide sequence ID" value="XM_009267299.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUB9"/>
    <dbReference type="GeneID" id="20564694"/>
    <dbReference type="KEGG" id="ero:EROM_110950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110950"/>
    <dbReference type="HOGENOM" id="CLU_010194_3_0_1"/>
    <dbReference type="OrthoDB" id="10267115at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="301" mass="34268" checksum="85B846794A6E980D" modified="2012-10-03" version="1">MTAVLLLFGALIVIYFFLRHRARHQRHEISNKKILVVGGSSGLGLAFAQILKEAGNNVTITSRSVQSLELLRSKWGFETKVLDVNTMDSQKSADFDYIFCCAGISHPSYFADQSFELFKETMNTNYLGTVAVLKHYTAINKKPMRFIMIGSTLSLLTFPGFSSYSPSKAALLSLFYTLRDEMKELGVELYFYNTASIQTPGFEMENKTKPAYTKAIEGMISPSSARERAFSFLSGMKRRSVVASDAFTYLCQIRFECECLIDYLLFPVAVVAVFMSRIYVRWRFKAEKKMRGKEDVYIKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUE3</accession>
    <name>I7AUE3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transport protein particle complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265599.1">
        <property type="nucleotide sequence ID" value="XM_009267324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUE3"/>
    <dbReference type="GeneID" id="20564720"/>
    <dbReference type="KEGG" id="ero:EROM_111210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111210"/>
    <dbReference type="HOGENOM" id="CLU_142964_0_0_1"/>
    <dbReference type="OrthoDB" id="10254842at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990070">
        <property type="term" value="C:TRAPPI protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14943">
        <property type="entry name" value="TRAPPC5_Trs31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016696">
        <property type="entry name" value="TRAPP-I_su5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902">
        <property type="entry name" value="41-2 PROTEIN ANTIGEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20902:SF0">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="148" mass="16823" checksum="DEE1991A26F89360" modified="2012-10-03" version="1">MSYLVCGMVEYLMEQRKEVEADLKAIGYEVGVKLLEICNFEREVHIPTLLYRITFDFLSLASDSDKRIEKAKDIDKTYLLTDTDGIFSKFMSVPNEWDGFSADSIVCGMIQAALMASGYNSEVVAYPKPSESFPNRIIFQIKILDPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGV4</accession>
    <name>I6ZGV4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263915.1">
        <property type="nucleotide sequence ID" value="XM_009265640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGV4"/>
    <dbReference type="GeneID" id="20520701"/>
    <dbReference type="KEGG" id="ero:EROM_010740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010740"/>
    <dbReference type="HOGENOM" id="CLU_119644_0_0_1"/>
    <dbReference type="OrthoDB" id="2194310at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="162" mass="18624" checksum="87961B32D71891D8" modified="2012-10-03" version="1">MVRERLTKEDEENIDIILNPYPLATENTLKGIDASNDPEVRNGLVNDLSVILSNYAAALNPKVQEKFPKLVGLLKDKDIYNASAFMLSDACRHMEDVQNAFRALGVFELLDFTIDHYRATTSLVYSLCMENKPNTIYFLEKYYNEERDKNSTLMQNVKDQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH25</accession>
    <name>I6ZH25_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GIT Spa2 homology (SHD) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263990.1">
        <property type="nucleotide sequence ID" value="XM_009265715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH25"/>
    <dbReference type="GeneID" id="20520776"/>
    <dbReference type="KEGG" id="ero:EROM_020180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020180"/>
    <dbReference type="HOGENOM" id="CLU_762971_0_0_1"/>
    <dbReference type="OrthoDB" id="5588096at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GIT Spa2 homology (SHD)" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="363" mass="41889" checksum="F64ED79167C91A0C" modified="2012-10-03" version="1">MGNEKNKEIIRCELENYVAEEMKNASPSTRQMDAIQKMVSLPEKNFEDLVSDIVNEMRRRSSVPFISPETPMQYKLSRIHEDGFKSLVLDLLLVMNQRSPEDKCSSENVHGLIDSLDKLISSLKEDMMNEEKMAKDICSSCDVSEKFMMFIEYSRNVFERNGEDTKVHEHMVKEAKEYFEIQTADGPDLMISVDMLLKKCDKLKYSNLPEYKYHRNNIQRLKVMDLEESVKKRLIRDEVVQVYSIFVMESTNLRGAREKQLECKVNSLVDVLCKIKNDAEEGREIAAYEYLEDVASSSGDILELVENMDLESKAYLSQLKMKVAALEQIHNRSSDEEPLPAMFSTIEAVKKVLEDVCRIQGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHD9</accession>
    <name>I6ZHD9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_021430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264115.1">
        <property type="nucleotide sequence ID" value="XM_009265840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHD9"/>
    <dbReference type="GeneID" id="20520905"/>
    <dbReference type="KEGG" id="ero:EROM_021430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021430"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="10">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="81"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="270"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="424" mass="47408" checksum="E1C145AAE59CAE88" modified="2012-10-03" version="1">MEEKSLIQDDKEVARELFTMIKRDTTAIETGLDENISGVGTDRGKLLVAEQFSDMGLSDELLKAIYNKGFEKPSLIQKSAIPHILRGHNVVVQSKSGTGKTIAYTCGILGNTRAGEGTQALVVTPTRELSTQVTEVISGLAKSLGMKVFSALKNKATDSVMGEIVVGSPGTILKLIDLGKLNHKEIRTIVLDEADILLDKDMMGTQTFRILKLVSGAQIVFFSATYSEQVKQTIEFYAPDAIKMYEERNGKPDEIKLFYIELEGDNKRKALKSLYEYLSISQMIIFVSSKATVNYLRKKLEDDLHSVSCLHGDLEIEERERAVEEFRSSKSKILLTTDVFSRGMDIPQVNLIVNYDLPIYKGIASTQTYIHRIGRSGRFGRTGFVVDFVRDEELEGYLKFQKELKFASKKFTIRALEEIAAELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHR6</accession>
    <name>I6ZHR6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin and thiamine biosynthesis E1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264235.1">
        <property type="nucleotide sequence ID" value="XM_009265960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHR6"/>
    <dbReference type="GeneID" id="20521029"/>
    <dbReference type="KEGG" id="ero:EROM_031160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031160"/>
    <dbReference type="HOGENOM" id="CLU_013325_1_0_1"/>
    <dbReference type="OrthoDB" id="10261062at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate-cyanide sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042292">
        <property type="term" value="F:URM1 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00757">
        <property type="entry name" value="ThiF_MoeB_HesA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF102">
        <property type="entry name" value="ADENYLYLTRANSFERASE AND SULFURTRANSFERASE MOCS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="236"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="329" mass="36706" checksum="33D21E6C597A79DB" modified="2012-10-03" version="1">MYLVSCGIGRLGLVDFDRVEIHNLQRQIIYTEADLSQYKVVAALSFARRANSCIEVVGYNEFLNKENVERIISPYDVILDCTDSIHTRYLLSDHCKALSKSLICGSVLRWEGQLYKLTPSGPCYRCLFPNMKGRTLTCEDAGVVGPVCGVIGSLQALEAIKIITGDTRPKMTIYNGMTSDLMDFQLRAPKPDCRVCSRRSIPEDLSFQVPRCPHIANTELIDPNIGTIDWENVLKNLKSYLLIDIRSPVQYEMFRVKGSVNIPLVNLEDKVRTIETGGKRVGVVCRRGISSKKGALIFKANGIDAFSVNGGIDGLKSYLRKMNEPTISE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI25</accession>
    <name>I6ZI25_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_041010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264365.1">
        <property type="nucleotide sequence ID" value="XM_009266090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI25"/>
    <dbReference type="GeneID" id="20521165"/>
    <dbReference type="KEGG" id="ero:EROM_041010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041010"/>
    <dbReference type="HOGENOM" id="CLU_1928233_0_0_1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051775">
        <property type="entry name" value="Homeobox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323">
        <property type="entry name" value="CEH-10 HOMEODOMAIN-CONTAINING HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323:SF7">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="34"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="133" mass="15753" checksum="FADE15712A4FAA51" modified="2012-10-03" version="1">MSTRSRFNKDEKKVVKFIDSTEEIIKHLNKTDSNKRSRLKLSGHQIDILESNFKIDSHPNSTTKSLLANTLSIPFKNIQIWFQNRRAKEKIARDNCKRRNGSTRIEGKEHEHGRMNYQAMCPFDFPPQERYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI44</accession>
    <name>I6ZI44_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acylglycerol-3-phosphate acyltransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264390.1">
        <property type="nucleotide sequence ID" value="XM_009266115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI44"/>
    <dbReference type="GeneID" id="20521190"/>
    <dbReference type="KEGG" id="ero:EROM_041270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041270"/>
    <dbReference type="HOGENOM" id="CLU_041844_6_1_1"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="78"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="282" mass="33058" checksum="D2A7F93CCC72858B" modified="2012-10-03" version="1">MKKVFKILVKLIIFLGVMGMFILGAPFSILAYPLHILNKPLCIRLSTFFTYTLWTLISWMFNLSSTVHGSIDIGNESYFVISNHIGSVDFILINEIARKSGMISHVKYAVKDGLRVFPVFYQIIVYVGFLVLKRSFEKDKKKIIRYLEFFNTSGIPIWFVFYPEGSRFSEELKLKSWKYSDEKGMARLNNVLFPRYKGFKLICENLKNSRIKKIVDVTFSYSENEVPPLWKFLFCDTTGIFNCDIRVVSIDEIDDYEKFLYKSFERKDTLITKWNSNATKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIE1</accession>
    <name>I6ZIE1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264485.1">
        <property type="nucleotide sequence ID" value="XM_009266210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIE1"/>
    <dbReference type="GeneID" id="20521287"/>
    <dbReference type="KEGG" id="ero:EROM_050550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050550"/>
    <dbReference type="HOGENOM" id="CLU_801926_0_0_1"/>
    <dbReference type="OrthoDB" id="2190962at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="353" mass="39121" checksum="24A128F55495B9C8" modified="2012-10-03" version="1">MDTVLVKLVDRSHLDVEHDPLHVSPSFCVEDLERYLRSVLSGDGGYRFYIEGRLLEGCLQKEIEERGLEVERGVIIEYEVDGHCTPDATVEVEDSISFLNVVKNDIWEIWCGTYGGRLQIYAYQDGSIVLKKATEYKKVRGVARGDRLYIYNSDGEVLSLRSGAVLFKVNGEITCSASSCSIVSVGTHEGECYVFKGRLMKVHKFKACISVTIIDKDEVMFVCMDGSMGVFETSTNAFRTKDFGYNITSGDARGNERVFGTSCSGLIVESKDHSTFVPTNIRFSNKVLMYNSCVVAQASQHTISVINAKNSQELRRISTEGYISDIGWAGNLLVVGVGSNLQGYIIHNLGGNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIM5</accession>
    <name>I6ZIM5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor eIF2B subunit gamma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">eIF2B GDP-GTP exchange factor subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_051430</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on the eukaryotic initiation factor 2 (eIF2) complex gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B gamma/epsilon subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264570.1">
        <property type="nucleotide sequence ID" value="XM_009266295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIM5"/>
    <dbReference type="GeneID" id="20521375"/>
    <dbReference type="KEGG" id="ero:EROM_051430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051430"/>
    <dbReference type="HOGENOM" id="CLU_049753_0_0_1"/>
    <dbReference type="OrthoDB" id="10250549at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051960">
        <property type="entry name" value="eIF2B_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056818">
        <property type="entry name" value="GlmU/GlgC-like_hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45989:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24894">
        <property type="entry name" value="Hexapep_GlmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="10">
        <location>
            <begin position="5"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-1-phosphate adenylyltransferase/Bifunctional protein GlmU-like C-terminal hexapeptide" evidence="11">
        <location>
            <begin position="275"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF24894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="364" mass="40558" checksum="C3FC2175F9664670" modified="2012-10-03" version="1">MFEVIILIGPGTELFPIVSEKFSKACLPIMNSPMILHTMECLRSISKKFFVVGLNEEKEDLMNAIKGRIDVPVEYVGIDTYDGTVASLLNVYPRISSEDVLVCKGDIVTNMDIQAMASNYFNEKKMFMVILENSTSETSILGYKGDDLMFYSNDSNEEIPFHLLKKGLTLTKDLDILQLYMFRTSLFKAFKPDYFSFKHGLLPNIVKSLIPTNPVGIHRPKKSYIYQIRTINNYLCVNALLKRRAVSTDKKLKTSESNAKFIKDYVKKYNLKDLSNVVGSNTKTDNVLLLDSIIGSGCDIGEESKIISSIVMNNVSIGNGSHIEGSVIGMGANIFSGSTLVNCKVSPGYVFTEVVDADTRSFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ69</accession>
    <name>I6ZJ69_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_070220</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264770.1">
        <property type="nucleotide sequence ID" value="XM_009266495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ69"/>
    <dbReference type="GeneID" id="20521581"/>
    <dbReference type="KEGG" id="ero:EROM_070220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070220"/>
    <dbReference type="HOGENOM" id="CLU_727676_0_0_1"/>
    <dbReference type="OrthoDB" id="267397at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF3">
        <property type="entry name" value="FI07626P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="4">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="292"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="351" mass="39284" checksum="B3066B4957C2EBA4" modified="2012-10-03" version="1">MKINIQHLKNKFVVEVENLDITIEELKKKLEEAMGESVRIPASDQTLIFEREKLDDGKKLSDYVKGSTEEIKLFVIKQNKNPNPISKPSNIYQNANTSDVQSQAHIGGNPHAGQSMPNPYAAFVPPYGGYGSPQQMYGQMPYGYGSPQQMYGQMPYGYGFPQGGGSPGQSDGYNEAFSNAMMQQMEQMLNNPTLLDQVLNIQNPNMTSEQKEEQKKMLKEALNMMKANPSLFQQALTPERLSWALNTMNGGHGGMFPSYPPNMGGFNQRDPRAPWMHGYPPPGYGSPRPNQQVDEQVYLVQLEQLKAMGFEDEDATKALKEARGDINRALDILHENKKRKNQETSGSSPKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ77</accession>
    <name>I6ZJ77_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">RNA exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_070320</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the REXO1/REXO3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264780.1">
        <property type="nucleotide sequence ID" value="XM_009266505.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ77"/>
    <dbReference type="GeneID" id="20521591"/>
    <dbReference type="KEGG" id="ero:EROM_070320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070320"/>
    <dbReference type="HOGENOM" id="CLU_054620_1_0_1"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06145">
        <property type="entry name" value="REX1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034922">
        <property type="entry name" value="REX1-like_exo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047021">
        <property type="entry name" value="REXO1/3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801:SF115">
        <property type="entry name" value="FI18136P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12801">
        <property type="entry name" value="RNA EXONUCLEASE REXO1 / RECO3 FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="7">
        <location>
            <begin position="146"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="370" mass="42666" checksum="03BD8B9E00A60441" modified="2012-10-03" version="1">MAKVRELKRLTVYDIQRILCWVFNLSKRPSFLPLRASDRPNTINIIKLEDSPEDISKLLSKLKICHTALVNEHLPFDDIIECILRDGEYENITPSLEYVKMENLGFIPFVGFEDIYRSGIFVDDEKSLSKVDVSGYRKALMPRSYDIIALDIEKVRTKAGKVPGRITMVDCNGKTIYDKILKPEDPVIDYLTKYSGLTKEIIDEGVDIDLAKNEIFNFIGTNTVIVGHGVENDLDSLRLYHEKIIDTAHLFSSPLGRKISLAQLSRTYLSKDIHLETHDSRIDALTCLELLSVKIQHMLRIMDPEAPRLNIQAVIERRCISDLLEHEQGHVNITTCNYRDLVRFLRSYRKAKECLWMLVYRVDGNIYLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJS5</accession>
    <name>I6ZJS5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endonuclease III homolog</fullName>
            <ecNumber evidence="12">3.2.2.-</ecNumber>
            <ecNumber evidence="12">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <shortName evidence="12">DNA glycosylase/AP lyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NTH1</name>
        <name evidence="14" type="ordered locus">EROM_080850</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="12"/>
    <dbReference type="EC" id="4.2.99.18" evidence="12"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265000.1">
        <property type="nucleotide sequence ID" value="XM_009266725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJS5"/>
    <dbReference type="GeneID" id="20521820"/>
    <dbReference type="KEGG" id="ero:EROM_080850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080850"/>
    <dbReference type="HOGENOM" id="CLU_012862_4_2_1"/>
    <dbReference type="OrthoDB" id="2099276at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03183">
        <property type="entry name" value="Endonuclease_III_Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004035">
        <property type="entry name" value="Endouclease-III_FeS-bd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030841">
        <property type="entry name" value="NTH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00764">
        <property type="entry name" value="ENDONUCLEASE_III_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="13">
        <location>
            <begin position="57"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="265" mass="30184" checksum="384A0CF6FE06FDE1" modified="2012-10-03" version="1">MKSESKKEIEGPLELYFEIKKQRENIVSPVDTMGCFITPPYRTEEERRFHILVSLLLSSQTKDEVTHEAMERLRELLPENAAIDDNIRRGLTIENIVNSSVDHINECIKRVGFHNKKAENLKKIAEILKKKGLPKRMEDLTSLPGIGNKMAILYMNHACGKVVGISVDTHVHRISNRIGLVKTRDVEDTREGLEKIIPKKEWKTINRILVGYGQTICVARRPRCEECCIRSRCPSSLFNRKASALKEEIFYSVTSIAPVSTISPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK19</accession>
    <name>I6ZK19_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_081940</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265105.1">
        <property type="nucleotide sequence ID" value="XM_009266830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK19"/>
    <dbReference type="GeneID" id="20521929"/>
    <dbReference type="KEGG" id="ero:EROM_081940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081940"/>
    <dbReference type="HOGENOM" id="CLU_000288_118_2_1"/>
    <dbReference type="OrthoDB" id="10020333at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14019">
        <property type="entry name" value="STKc_Cdc7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="334" mass="37927" checksum="382B98790A02C2C4" modified="2012-10-03" version="1">MQKYTPIEKIGEGSFSVVYKALDAESGRYVALKAITRTSSPARILEELMFLKALEGKRNCMGLLGVFRNEDQVVAVFPYFKPVDFREFISNAGLADIKQYLYNLLVAIDHVHSNGIIHRDLKPGNFLYNKEAGRGMLIDFGLAQYEDCKEDQARDDSSKSSTPLLFFNSIVSKAKPPGYYERDGRPQMKAPRAGTRGFRAPEVLFRHQRQTRAIDMWSIGVIFLTILTAQYPFFYSSDDVDSIVEIATIFGHAEMRKAARFYGRLWRSNIDTIPEERIPFERIVESLNPWAEVGAEGYDLLYRMLDLCSSSRITASDALSHPFFDGLRTHGDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK67</accession>
    <name>I6ZK67_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">TBP-associated factor 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_090260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265140.1">
        <property type="nucleotide sequence ID" value="XM_009266865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK67"/>
    <dbReference type="GeneID" id="20521964"/>
    <dbReference type="KEGG" id="ero:EROM_090260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090260"/>
    <dbReference type="HOGENOM" id="CLU_078328_0_0_1"/>
    <dbReference type="OrthoDB" id="21060at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08045">
        <property type="entry name" value="HFD_TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007900">
        <property type="entry name" value="TAF4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05236">
        <property type="entry name" value="TAF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID component TAF4 C-terminal" evidence="9">
        <location>
            <begin position="69"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF05236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="305" mass="36040" checksum="EE70F41D61534671" modified="2012-10-03" version="1">MNKEAFERLPQEKKKIIETAYFSAVRKEISASQFFGVCKEALTKEQFESLFSNNTPNKEQQEEMKTEHLQDIIQYSGVDLKEEAEHIVRETETNINFGEYDEEDANDQIGSLLNVPQFREFIARIGNSRRVGVSEESFFLLFQVVRRKILDTVEKMEESSKWRVEYNLSEFIIRINNDLSRQLWCLEQIEKAELEKFTIRRGEEEGKKKVKKTIQEREDLVIKKRLSNTVALAALGVQQKSWMSAEDVRVNEESTAFNSIYSPFDEKALERKVANRTITMKDLLYVLERDRRYNKSIFTIQHYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKA1</accession>
    <name>I6ZKA1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265190.1">
        <property type="nucleotide sequence ID" value="XM_009266915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKA1"/>
    <dbReference type="GeneID" id="20564300"/>
    <dbReference type="KEGG" id="ero:EROM_090760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090760"/>
    <dbReference type="HOGENOM" id="CLU_026126_10_1_1"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02066">
        <property type="entry name" value="GRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="17"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="98" mass="11300" checksum="73CFF34F7A68757F" modified="2012-10-03" version="1">MKKGELEKALSRNANLVVSKRGCPFCLDAVKTLNEWKIEFDEIKNTENEELSKEIEEEYKFPTFPKIFLNKKFIGGASDLRKYVTTKEFLDAFGSGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKI7</accession>
    <name>I6ZKI7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265305.1">
        <property type="nucleotide sequence ID" value="XM_009267030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKI7"/>
    <dbReference type="GeneID" id="20564418"/>
    <dbReference type="KEGG" id="ero:EROM_091940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091940"/>
    <dbReference type="HOGENOM" id="CLU_062477_0_0_1"/>
    <dbReference type="OrthoDB" id="2191863at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="242"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="326" mass="38437" checksum="94CA6D520B1AB5A2" modified="2012-10-03" version="1">MSRDLRFSIWAKCIGSREKCIELKPIGLRNANKGQNPRHQVGENGKIEDKDLVKFILDLYRDIEGNNQENIFNILKNLERDFPMVYNLKLLALFIMHILELPYASPQAVYRFIHSLMESHNFCSVFSRDQGIKPDSKVNEDKEDNTHSAFWKTFICDYPLVFDQMVDLLLCYEGSNIESVPEFLYKQTGGNDLREWIKFMSIRSNLEKLGDVFDSERLETPVGVDRREETYFDFLVVQNEAIKAQEMMKIEASERLNSLQEERNELYAQKINLMKACSSLESDLKEYQEEYVKKVEEMLHDVEDKCERYEKENKELKAKLEEGKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKL5</accession>
    <name>I6ZKL5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">mRNA cap guanine-N(7) methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">mRNA (guanine-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">mRNA cap methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_100260</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:67008"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="Rhea" id="RHEA-COMP:17167"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:156461"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.1.1.56"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.56" evidence="2"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265340.1">
        <property type="nucleotide sequence ID" value="XM_009267065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKL5"/>
    <dbReference type="GeneID" id="20564456"/>
    <dbReference type="KEGG" id="ero:EROM_100260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100260"/>
    <dbReference type="HOGENOM" id="CLU_020346_1_2_1"/>
    <dbReference type="OrthoDB" id="10248867at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004482">
        <property type="term" value="F:mRNA 5'-cap (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004971">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016899">
        <property type="entry name" value="mRNA_G-N7_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039753">
        <property type="entry name" value="RG7MT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189:SF2">
        <property type="entry name" value="MRNA CAP GUANINE-N7 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12189">
        <property type="entry name" value="MRNA GUANINE-7- METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03291">
        <property type="entry name" value="mRNA_G-N7_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028762">
        <property type="entry name" value="ABD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51562">
        <property type="entry name" value="RNA_CAP0_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 15" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MRNA cap 0 methyltransferase" evidence="16">
        <location>
            <begin position="26"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="35"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>mRNA</name>
            <dbReference type="ChEBI" id="CHEBI:33699"/>
        </ligand>
        <ligandPart>
            <name>mRNA cap</name>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="15">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="15">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="15">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="15">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="15">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <feature type="site" description="mRNA cap binding" evidence="15">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR028762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR028762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AFN83843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="282" mass="33110" checksum="DA9BA5C73E97E99D" modified="2012-10-03" version="1">MKNKKEEIRKHYNSIKERGREVRQRSRAINIRNTNNFIKACLIRLYTKKGDTVLDLGCGKGGDLLKYEKAGIDEYYGIDIAEISIRDAYVRAENMKRRFKVSFKAQDVYNQHISLGRQFDIVSSQFSFHYAFSSDESLDISLGNVVRHLKPGGYFIITVPSKEVILERYRRGKISNEFYKIEIDKKEDAPIESVKEYRFTLVDSVNNCVEYFVEFTKMLDGFKKLGVVLIERKGFIDFLEEESRRNQELFKHMRVRRLGKEEAEVVGTYEVMVFQKLNKSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKM5</accession>
    <name>I6ZKM5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proteasome alpha subunit C6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265355.1">
        <property type="nucleotide sequence ID" value="XM_009267080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKM5"/>
    <dbReference type="GeneID" id="20564472"/>
    <dbReference type="KEGG" id="ero:EROM_100420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100420"/>
    <dbReference type="HOGENOM" id="CLU_035750_4_0_1"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="2">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="234" mass="26002" checksum="38EBB12850686F40" modified="2012-10-03" version="1">MTTQEEISNIFNSDGKLLQIEYGLEAVNNALPIVTVKSRDMVVCASKKVPQGKLEESSPTSFYRVSEKCYVGITGLPGDIDYVIKRIKMVANRKTFSLGTEVTPDILCRSFADKIQQLIQSSEERSAAFNASIFGFDREKPMIYQTDMSGISYPCYATASGEKHGKMTKFIEKSYRKDMEGKELFEVAIGALLESIGTDSGCSEIEVCYLKAGQDLCYLSAKEIDKILQDIAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL01</accession>
    <name>I6ZL01_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_110140</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265495.1">
        <property type="nucleotide sequence ID" value="XM_009267220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL01"/>
    <dbReference type="GeneID" id="20564613"/>
    <dbReference type="KEGG" id="ero:EROM_110140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110140"/>
    <dbReference type="HOGENOM" id="CLU_451335_0_0_1"/>
    <dbReference type="OrthoDB" id="28053at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050840">
        <property type="entry name" value="Adaptor_Complx_Large_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22780">
        <property type="entry name" value="ADAPTIN, ALPHA/GAMMA/EPSILON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="599" mass="68544" checksum="0A511CA114126AB4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL37</accession>
    <name>I6ZL37_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_110560</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265535.1">
        <property type="nucleotide sequence ID" value="XM_009267260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL37"/>
    <dbReference type="GeneID" id="20564655"/>
    <dbReference type="KEGG" id="ero:EROM_110560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110560"/>
    <dbReference type="HOGENOM" id="CLU_029499_0_0_1"/>
    <dbReference type="OrthoDB" id="1733332at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00930"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03356">
        <property type="entry name" value="LbH_G1P_AT_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06425">
        <property type="entry name" value="M1P_guanylylT_B_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000013">
        <property type="entry name" value="mannose-1-phosphate guanyltransferase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045233">
        <property type="entry name" value="GMPPB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050486">
        <property type="entry name" value="Mannose-1P_guanyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22572">
        <property type="entry name" value="SUGAR-1-PHOSPHATE GUANYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="9">
        <location>
            <begin position="9"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="10">
        <location>
            <begin position="257"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN84038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="346" mass="38992" checksum="8E9F856B8AF0C320" modified="2012-10-03" version="1">MVHVKEQVKAVILVGGYGTRLRPLTYTVPKPLVPFANKPILRHQIEALVGAGIKEIILALNYYSELIIREVRDYSNEFGINVIYSKEQEPLGTAGPLALARKYLEGHTFFVLNSDITCRFPLREMLSFHLSHGKEGTILSTGVEDPSRYGLIITEENTSLVQTFLEKPKNATSNRINAGIYILNPSVLDRVELRECSIEREIFPKMAEERQLQVFDLEGFWMDIGQPADYIKGQGMYLKYYQEAAMCDYLSDKKMFSIESNVVIGKNVKIGKNVTITNSTIFDNVEIGDNVIIKDSIVGWNTRIEDNATINSCCVLGYATTVERFSILSSVKTLPNKSSFKLLEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLF3</accession>
    <name>I6ZLF3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_111570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265635.1">
        <property type="nucleotide sequence ID" value="XM_009267360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLF3"/>
    <dbReference type="GeneID" id="20564756"/>
    <dbReference type="KEGG" id="ero:EROM_111570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111570"/>
    <dbReference type="HOGENOM" id="CLU_159444_0_0_1"/>
    <dbReference type="OrthoDB" id="2186267at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="130" mass="15504" checksum="68757AB8DC9217E7" modified="2012-10-03" version="1">MMLYRVMDLIVREDTFKLFTSRTGAGIFERLEEAPRMETMEDLEERIKGYFKEVLRVSPTDSLYFNEGFRMLNFVEKIFKELRELNESAMERSIRSPMDDVIRLYSESLNKFVEGMDIPVLQEHVKFIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS37</accession>
    <name>I6ZS37_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263908.1">
        <property type="nucleotide sequence ID" value="XM_009265633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS37"/>
    <dbReference type="GeneID" id="20520694"/>
    <dbReference type="KEGG" id="ero:EROM_010670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010670"/>
    <dbReference type="HOGENOM" id="CLU_102724_3_2_1"/>
    <dbReference type="OrthoDB" id="295715at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="111" mass="12765" checksum="C7D26FD23320DAA9" modified="2012-10-03" version="1">MNIIAELNDYQKNICLEFILIKYLGTNKTKGNDKIDVWLVGDESGTIEFGVWNCSLSPGDVIHLSGGYTSLFQGKKRLFISKTGWINRVRTFRKIFKVSECHEEILKEFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS42</accession>
    <name>I6ZS42_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263913.1">
        <property type="nucleotide sequence ID" value="XM_009265638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS42"/>
    <dbReference type="GeneID" id="20520699"/>
    <dbReference type="KEGG" id="ero:EROM_010720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010720"/>
    <dbReference type="HOGENOM" id="CLU_1927571_0_0_1"/>
    <dbReference type="OrthoDB" id="2121326at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="13"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="14855" checksum="10688EF1DE3FC833" modified="2012-10-03" version="1">MPIIVRDCSGMSPEEIKAKVKELGRPSLLKFSSENCGPCEDLKEKLSQYSTDLEINVFEICRTRTASYPSLWDEFDITAFPTVVLVDKDMKELQDKNGSQGALTRIVGSNIKKFEELVKEHRHLFGQNTSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS73</accession>
    <name>I6ZS73_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ca2+-dependent lipid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_011070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263948.1">
        <property type="nucleotide sequence ID" value="XM_009265673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS73"/>
    <dbReference type="GeneID" id="20520734"/>
    <dbReference type="KEGG" id="ero:EROM_011070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011070"/>
    <dbReference type="HOGENOM" id="CLU_011052_0_0_1"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="8">
        <location>
            <begin position="122"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="860"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1018" mass="116588" checksum="9C9B96AC3FA6E9C1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSA2</accession>
    <name>I6ZSA2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plasmid maintenance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lipin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263983.1">
        <property type="nucleotide sequence ID" value="XM_009265708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSA2"/>
    <dbReference type="GeneID" id="20520769"/>
    <dbReference type="KEGG" id="ero:EROM_020110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020110"/>
    <dbReference type="HOGENOM" id="CLU_002546_4_0_1"/>
    <dbReference type="OrthoDB" id="4567at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026058">
        <property type="entry name" value="LIPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007651">
        <property type="entry name" value="Lipin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013209">
        <property type="entry name" value="LNS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031315">
        <property type="entry name" value="LNS2/PITP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181">
        <property type="entry name" value="LIPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12181:SF12">
        <property type="entry name" value="PHOSPHATIDATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04571">
        <property type="entry name" value="Lipin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08235">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00775">
        <property type="entry name" value="LNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LNS2/PITP" evidence="2">
        <location>
            <begin position="391"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="595" mass="68639" checksum="E204ABA24F248DB5" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSZ8</accession>
    <name>I6ZSZ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264218.1">
        <property type="nucleotide sequence ID" value="XM_009265943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSZ8"/>
    <dbReference type="GeneID" id="20521012"/>
    <dbReference type="KEGG" id="ero:EROM_030995"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030995"/>
    <dbReference type="HOGENOM" id="CLU_188586_0_0_1"/>
    <dbReference type="OrthoDB" id="2191508at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="84" mass="10383" checksum="9D60236591549C0A" modified="2012-10-03" version="1">MFLPPDTNTRERRRFDLDDLRVYYLICEELGIAEEEHVQKSFYYLMKWAGQDKFSGEIGFLRNYIMRIKKERRDKHGEWDMLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTI6</accession>
    <name>I6ZTI6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ser/Thr protein phosphatase PP1-1 gamma catalytic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_050430</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264473.1">
        <property type="nucleotide sequence ID" value="XM_009266198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTI6"/>
    <dbReference type="GeneID" id="20521275"/>
    <dbReference type="KEGG" id="ero:EROM_050430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050430"/>
    <dbReference type="HOGENOM" id="CLU_004962_5_2_1"/>
    <dbReference type="OrthoDB" id="445564at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00144">
        <property type="entry name" value="MPP_PPP_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051134">
        <property type="entry name" value="PPP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45668:SF9">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF033096">
        <property type="entry name" value="PPPtase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="5">
        <location>
            <begin position="162"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR033096-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="457" mass="52344" checksum="4C91C706F1F0792D" modified="2012-10-03" version="1">MDIRQMEHLRQEANSLFKNQRIDEALDLYKKSFMEGMKFMVPGSWNGPLEEQLSLLAYNICIVYYKRKNFPKSLTFGLESLKRCKNDKVLHKICTIYLRMGMLKEYVEMYEQIVDKSCCREIKVLLNRMKLGSQTIEKYLENRITLEKLYEMSKEISEGRFIPADVLESILEQGESLLLGCENVVYVESKGEVLVFGDTHGQYFDVVGVLNKVFDGERIFIFNGDYVDRGTHSVENFVLLISLKILFPGKVHLTRGNHELMDINKAYGFFDEVKRKYPFSSGSVYQKFQAVFRALPISIIVNEKVFITHGGLPGTPTKIDELQESYRMTDAHTDELLKGFLWSDPEEITGIEESKRRAGVVFGIDIVDQFLQGNGLDLLVRSHQAIENGYKVNHEGKTVTVFSAPDYEGTESSGSYLVLYPSNGREDEVVNVSRLTKYKVGRFGKSKPGETLKLSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTS5</accession>
    <name>I6ZTS5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_051460</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264573.1">
        <property type="nucleotide sequence ID" value="XM_009266298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTS5"/>
    <dbReference type="GeneID" id="20521378"/>
    <dbReference type="KEGG" id="ero:EROM_051460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051460"/>
    <dbReference type="HOGENOM" id="CLU_055667_0_0_1"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="8">
        <location>
            <begin position="235"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="404" mass="46071" checksum="B8F46525D672DF81" modified="2012-10-03" version="1">MKYFSKGMFYTFFVLTYLAQMIEVLNIFITKSLVDSGETGLEEGLATNEMAKRDQGYSNSIEKTIGKRSVELLEDVLVHTMHFSASILFFTEAGRLFSMKMVGVIFGQIFDLEIVFLTIIVSMLSLNAFTEYVVSSIYKSPSLEMILVWCFFGVLFVIPILVFISIKLLKIFGIGFIVSCYLSYFIMEISDILSISNVDLSKMEKVPVSVFSENIQRLMNDRGLSDSIYKERKPGKNVNAALIGIGNSERIEIYGKVENIDDGQLESILIHEVGHSYDRSLIKKISVFFLLLGLEMLFLIILYDSVAKEFVWDNVSKEGSFTVLMCLYFASIRPWLFILYNLTSQSAEMYADLLTKKYNYNRTLASTLYKISVDSLDFLSPSWLYNSLNSLHPSIMSRIEYLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU00</accession>
    <name>I6ZU00_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_060310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264623.1">
        <property type="nucleotide sequence ID" value="XM_009266348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU00"/>
    <dbReference type="GeneID" id="20521428"/>
    <dbReference type="KEGG" id="ero:EROM_060310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060310"/>
    <dbReference type="HOGENOM" id="CLU_063953_1_1_1"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="197" mass="22376" checksum="2FC8FF93A094502A" modified="2012-10-03" version="1">MGVLFQGAESIIWDDGETVSKRRVRKAYRIEALDTKIINSRTRREARILKKLEVVGIPAPKLIDVHGNTIVMEKIDGMPLKEKIDDSDDQKALFYDLGVLVSKLHLADIVHGDLTTSNFIFRDKIHVIDFGLSYVSSKDEDKAVDLYVFEKAVGCRHDTKLLESFYEGYMANGSSDVLNKLETVRLRGRKRERTAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU29</accession>
    <name>I6ZU29_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4203 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264643.1">
        <property type="nucleotide sequence ID" value="XM_009266368.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU29"/>
    <dbReference type="GeneID" id="20521449"/>
    <dbReference type="KEGG" id="ero:EROM_060520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060520"/>
    <dbReference type="HOGENOM" id="CLU_1102769_0_0_1"/>
    <dbReference type="OrthoDB" id="2193794at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003706960" description="DUF4203 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="26885" checksum="836A1B32F9289A55" modified="2012-10-03" version="1">MNLVALIPFLSLALCSTPDSATPASDVPTTSSDGVGSLALATPNYAASGSSTAINTIYALVLLVAIAVINTPRKSLEQTQYSVTAVLGGYFSVSHILQSIGITSNTTMHIVAAVVGVVAAVMAYNKELRSLLFGLISAYATTYFVVLIVRLESTLYAGILGVVLFFVYIILGRMREDSRLLVSMAKADVSSLGFAAFVNVWGVLDLFGGMHGLNANEGLFSGFIIGGIIILMVFTIVFVMNYFQEWTEEKING</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU46</accession>
    <name>I6ZU46_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartyl-tRNA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_060730</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264663.1">
        <property type="nucleotide sequence ID" value="XM_009266388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU46"/>
    <dbReference type="GeneID" id="20521469"/>
    <dbReference type="KEGG" id="ero:EROM_060730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060730"/>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_1"/>
    <dbReference type="OrthoDB" id="372395at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="169"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="307" mass="34730" checksum="513B93A671F92B77" modified="2012-10-03" version="1">METQLSRISINGITEIKDLDGNLDGKCIRVRGFALKVSCTGKYVFIVLRDNGCTIQCMCEKPIGGKGSLTPQEFNLLKKVTHESFIEVKGKVVKQEKEISGCDKKDIEIHILGFDILSAADKNLPFVMKDASATAEEREKNPTLQNVGYHIRLDNRAMDLRTPQARATFRIIDGVMFFFRGYLRRNGFVEIKTPKLIGSSSEGGANLFSVDYFKRKAFMAQSPQLYKQMAIIGGLKRVCEIGHVYRAEESNINRYLSEFVGLDMEMEISTDYNDMIAFIHSMFVSIFDSLKEEYQRRTGNNQGLPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUG7</accession>
    <name>I6ZUG7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="12">1.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_070750</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="12"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264823.1">
        <property type="nucleotide sequence ID" value="XM_009266548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUG7"/>
    <dbReference type="GeneID" id="20521634"/>
    <dbReference type="KEGG" id="ero:EROM_070750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070750"/>
    <dbReference type="HOGENOM" id="CLU_030140_0_1_1"/>
    <dbReference type="OrthoDB" id="1152826at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd18126">
        <property type="entry name" value="GAPDH_I_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05214">
        <property type="entry name" value="GAPDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000001">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836:SF76">
        <property type="entry name" value="GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 9 12" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="13">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="202"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="10">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="326" mass="35575" checksum="BF825B4145F280B1" modified="2012-10-03" version="1">MKVGINGLGRIGKLVYKVLRERGVEVPLVNDPHLKPEQVAYLLKYDSVFGKCKECQVEGDKIVYRGKKTCLSSFDKPESIPWTKYGVDAVIEASGVFTGLKECEGHKDVKIVVITAPSKDAPMFVYGVNHQDYVSQRVISNASCTTNCLAPLVKVIDDNFGIVEGLMTTVHAVTATQKIVDGIGKSSRRGRSGLSNIIPASTGAATSVGRVIPSLANKLNGMAMRVPVSNVSVVDLVCRTRKETSIEEIVKAIEKFSENNEGIIGTTRDPVVSSDFIGDPRSSVLDINASISMGSNFFKLISWYDNEYGYSCRVCDLLEYAYSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUS4</accession>
    <name>I6ZUS4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polyadenylate binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_080440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264963.1">
        <property type="nucleotide sequence ID" value="XM_009266688.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUS4"/>
    <dbReference type="GeneID" id="20521780"/>
    <dbReference type="KEGG" id="ero:EROM_080440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080440"/>
    <dbReference type="HOGENOM" id="CLU_669075_0_0_1"/>
    <dbReference type="OrthoDB" id="439639at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12320">
        <property type="entry name" value="RRM6_RBM19_RRM5_MRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="95"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="334"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="411" mass="47641" checksum="653E499F11F344DC" modified="2012-10-03" version="1">MRIVVKDLPLSTTKEEIEKEFSKHGRITDVFMARNGQGRFRRICFIGYMEEKEGVEAIKYRNGSLFKNQKIRCEALKEDIVQMGESEKRMVKYSRKIFIRNVPMEVSEQIIHDTFKEYGEIEEVGLLDLKEGKGAYVKFSEGECAVEAYRSVKTIGGMKARMYPWKDKAEKSQYEHYNTLFFSFESVVKRICESERIAAKDLVDINDKDLGARMALIETHLVQETKEFLENNGIYLDNLTGEIDKRTLIIRNMELMKCLDLVDSGCKISIAPSKCLALLKFEEEEEARKCYKKLSLKRMKEKVIYCEYAPVCNIPKDTKEEMPKDYSKKDRAINKLLVRNVPFQASEKEIRKIFDSFHVVDVRIPIKREGTSRGFCFVTLGSPDEVDAAIRHFGSSTHLYGRRLVLEKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUT7</accession>
    <name>I6ZUT7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264983.1">
        <property type="nucleotide sequence ID" value="XM_009266708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUT7"/>
    <dbReference type="GeneID" id="20521803"/>
    <dbReference type="KEGG" id="ero:EROM_080670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080670"/>
    <dbReference type="HOGENOM" id="CLU_353369_0_0_1"/>
    <dbReference type="OrthoDB" id="2191774at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="795" mass="91659" checksum="346020F205F42435" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV11</accession>
    <name>I6ZV11_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ricin B lectin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265083.1">
        <property type="nucleotide sequence ID" value="XM_009266808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV11"/>
    <dbReference type="GeneID" id="20521905"/>
    <dbReference type="KEGG" id="ero:EROM_081700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081700"/>
    <dbReference type="HOGENOM" id="CLU_1288887_0_0_1"/>
    <dbReference type="OrthoDB" id="2192951at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26113" checksum="B4C91F247A0B1184" modified="2012-10-03" version="1">MKKAMMIFQLMEAISGLSIFAKGGVKKYITKGGRWRSPDYGINYQLAAMSTSGTPTEFRSEELSPGIKILKEVGTSDVLDLHHPKSGQTKLIFHSRNNGLTQKFGIEGNENDGYTLKNKNKDLCLEYDDDGNIYGTTCSENSRQKFDVVYTPEDPEYKPPSEEAMDAPSIGSPSPQGTPQIFIFNESPENNHGSKHKHRRGHSHKHHHHHHHSPHESPYFTYQKYSDQIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV96</accession>
    <name>I6ZV96_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RhoGAP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265143.1">
        <property type="nucleotide sequence ID" value="XM_009266868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV96"/>
    <dbReference type="GeneID" id="20521967"/>
    <dbReference type="KEGG" id="ero:EROM_090290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090290"/>
    <dbReference type="HOGENOM" id="CLU_806602_0_0_1"/>
    <dbReference type="OrthoDB" id="2196166at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="158"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="349" mass="40385" checksum="0B9AF0115F952119" modified="2012-10-03" version="1">MLRDFPKKRKIFNIAEMKEIEETHAKGDEPCSESNASPSVHPQPYSPRVILQPISESDHTPSNYLKNGSVQENIGGFRNSSARALYERLFSSTSIQRKKKLETAVFPSEKSEEELLEFRYEFLSSSQKIELSRFCWNAFRKRSTNTLFAWMNCFWKKSSIGRCRLINPMVFDVIESLKRRAVCTKFIFRYEPDKSEASRIANMVHEDKCVDIEGTNVLVLANALKNYIREHLDGLIPIEVFEKIKSCIISNDRHRKEALFSYMPFIFTDAERRLLVSLFELLRIIDENSDVTEMTIDSLLGLFSLVLHPQAAFTTLHDLDHAKTITREMYRLDFGAIPQGVVVHELVFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVE3</accession>
    <name>I6ZVE3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rho-GAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265193.1">
        <property type="nucleotide sequence ID" value="XM_009266918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVE3"/>
    <dbReference type="GeneID" id="20564304"/>
    <dbReference type="KEGG" id="ero:EROM_090800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090800"/>
    <dbReference type="HOGENOM" id="CLU_1137992_0_0_1"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="1">
        <location>
            <begin position="61"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="244" mass="27860" checksum="CE1286083CA6FAA0" modified="2012-10-03" version="1">MGTTNGNKGSKKILYESLCTVEAENLSEACNSAIEGERPPTGKRAQILRFIGEMCCCGDMFGADTFINESVFSAMGYIEKNGWKVKGIFISSESEENVNNCVKLLELNEKIDYPSVHPKDVGMGFKIYVEENLKNIIPLNVRKRVIEAYKNKDDERKEIIIPRIPFVLSDPNRMFLKSLKNIFLAIETNTGENEVDIDEIYRQFGPLIIRRPSEMLDTDTDILKMILMDLMKVDFDEFPISFYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW59</accession>
    <name>I6ZW59_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Dephospho-CoA kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110120</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265493.1">
        <property type="nucleotide sequence ID" value="XM_009267218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW59"/>
    <dbReference type="GeneID" id="20564611"/>
    <dbReference type="KEGG" id="ero:EROM_110120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110120"/>
    <dbReference type="HOGENOM" id="CLU_057180_3_1_1"/>
    <dbReference type="OrthoDB" id="247245at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02022">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="187" mass="21309" checksum="BA6B7CCDE709E231" modified="2012-10-03" version="1">MRIIAITGGIGTGKTALLNILKARGQATINVDGLVHEILNGEDIKSIRQRFFTDPKFRKAHEKKIRPKIYIETAKRVIYLLLAGYSVVFIEIPLLFELDLHHYFYTIVVACDENLQMMRGRKISYLKERLSFQIPMEKKIKLAQEVIYNNGNIDDLIDSASDLNFSGSSIYCCLTIILTIILLVVCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW69</accession>
    <name>I6ZW69_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Exocyst complex subunit Sec6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265503.1">
        <property type="nucleotide sequence ID" value="XM_009267228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW69"/>
    <dbReference type="GeneID" id="20564622"/>
    <dbReference type="KEGG" id="ero:EROM_110230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110230"/>
    <dbReference type="HOGENOM" id="CLU_025213_0_0_1"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="690" mass="80394" checksum="232297AB8EB53CB7" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWJ0</accession>
    <name>I6ZWJ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">t-SNARE complex subunit syntaxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111450</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265623.1">
        <property type="nucleotide sequence ID" value="XM_009267348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWJ0"/>
    <dbReference type="GeneID" id="20564744"/>
    <dbReference type="KEGG" id="ero:EROM_111450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111450"/>
    <dbReference type="HOGENOM" id="CLU_082505_0_0_1"/>
    <dbReference type="OrthoDB" id="10255013at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006011">
        <property type="entry name" value="Syntaxin_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00804">
        <property type="entry name" value="Syntaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Syntaxin N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="274" mass="31836" checksum="6C5BA905463257BB" modified="2012-10-03" version="1">MQDFLKDSKRILNDIETLNSYTNSIDYLSSQKNSSVLSKEEEKGLNNQIDVLNERFRVLSRNIKERIDSFSSETTELEKDSKKDRFYHETRKIHLHAQSKKLTEAINRYRESQIRHREDEVDKFRLQYIIAKPDATEKEIEEFIGDDDGTKVASAYALGAHSAQGILEEAENRKKNIKKIEEMVQEIVDLLNLISQEVSKRTEVVEAINDDLITGEENTAAANADLESALAYQIRATRIKRVFVYGILIIGLIFLIYFTFKTNFFGAFDRIRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWJ7</accession>
    <name>I6ZWJ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ring zinc finger transcription repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_111550</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265633.1">
        <property type="nucleotide sequence ID" value="XM_009267358.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWJ7"/>
    <dbReference type="GeneID" id="20564754"/>
    <dbReference type="KEGG" id="ero:EROM_111550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111550"/>
    <dbReference type="HOGENOM" id="CLU_119160_0_0_1"/>
    <dbReference type="OrthoDB" id="1923159at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12438">
        <property type="entry name" value="RRM_CNOT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034261">
        <property type="entry name" value="CNOT4_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039780">
        <property type="entry name" value="Mot2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12603:SF0">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="26"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="4">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="2">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22952" checksum="6D2F06E5FDDF2041" modified="2012-10-03" version="1">MPKDPMLIASMQSGGSFSNIRVIQKNLVYIICIPQKYADEGVLSRHEFFGQFGAIKKIVVNKRTSSLESTASAYITYSTDEEAKTCIQEVDESLLDGKVLKCTYGTTKYCTFYLRNAICQNSDCMYLHENRSQKDILTKDEMCSSKHKLHEFEIRNKNKKRIGKRYDFDILNELFKHKTSRVFKAPERILFEPLDFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACP9</accession>
    <name>I7ACP9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263852.1">
        <property type="nucleotide sequence ID" value="XM_009265577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACP9"/>
    <dbReference type="GeneID" id="20520636"/>
    <dbReference type="KEGG" id="ero:EROM_010100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010100"/>
    <dbReference type="HOGENOM" id="CLU_1677870_0_0_1"/>
    <dbReference type="OrthoDB" id="2195362at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="158" mass="17786" checksum="D914F04A289E9109" modified="2012-10-03" version="1">MQVLRDELEKISDKYGYQELYVEGFTPEQIYYQIEDLCNSIVERSDVAISRLYPKYQKLSLQNDGKVCCGKSSEDSQKETIADQDEEIPPESIEIEKCSSVGRLHKDESLGSSSFDSSENDVFQMPASETFRLLESNAENASGHSSSGSEPFSEDDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACQ4</accession>
    <name>I7ACQ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribosome-associated chaperone zuotin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263857.1">
        <property type="nucleotide sequence ID" value="XM_009265582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACQ4"/>
    <dbReference type="GeneID" id="20520641"/>
    <dbReference type="KEGG" id="ero:EROM_010150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010150"/>
    <dbReference type="HOGENOM" id="CLU_082159_0_0_1"/>
    <dbReference type="OrthoDB" id="1690618at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051083">
        <property type="term" value="P:'de novo' cotranslational protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054076">
        <property type="entry name" value="ZUO1-like_ZHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044634">
        <property type="entry name" value="Zuotin/DnaJC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43999:SF3">
        <property type="entry name" value="TRANSCRIPTION FACTOR MAMYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21884">
        <property type="entry name" value="ZUO1-like_ZHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="1">
        <location>
            <begin position="234"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="295" mass="35788" checksum="5D54D6D1A2091C8D" modified="2012-10-03" version="1">MLNWIIRYEKPTGELARIHEEHLKRRNVLNLYTKREFERWGRCIDLYILLDLDMYRTKPIPSSILEHVVKVKMHEYHPDLIKGCREAFLLVKIARDVLRDRKLRLFYDSNFFDESIPEDKIYREDEFFDVFGECFQRNARFSINQPVPLLDRNDDPKKALEFYEFWGNFKSWRAFEPVEELYNMGEYDRSQYSIKNREKLSFLKNQDALRIKKLVQIAKKRDPRVGKSIEEQMKEMMRMNSWTPLEVSTLRRLISLVGKTKKNKWEMITEKLAEITKTKRSIKEVMEKGMEIEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD63</accession>
    <name>I7AD63_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Poly(A) polymerase</fullName>
            <ecNumber evidence="11">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_020650</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Polymerase that creates the 3'-poly(A) tail of mRNA's.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 2 magnesium ions. Also active with manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="11"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009265762.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20520825"/>
    <dbReference type="KEGG" id="ero:EROM_020650"/>
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        <property type="component" value="Chromosome II"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:mRNA processing"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:RNA 3'-end processing"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NT_sf"/>
    </dbReference>
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        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
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        <property type="entry name" value="PolyA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="17">
        <location>
            <begin position="11"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="16">
        <location>
            <begin position="209"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="15">
        <location>
            <begin position="353"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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            <note>catalytic</note>
        </ligand>
    </feature>
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        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <location>
            <position position="156"/>
        </location>
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            <name>ATP</name>
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        </ligand>
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        </location>
        <ligand>
            <name>Mg(2+)</name>
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        <location>
            <position position="217"/>
        </location>
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            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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            <begin position="235"/>
            <end position="236"/>
        </location>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN82540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="505" mass="58298" checksum="B900AB6E665EBE89" modified="2012-10-03" version="1">MEMSERNYKHGVTGPLSMKESTEEDVKLSEEMDMYLQERGFFEDDEEGQTRERVLGKLNFMVKEFVARMAKNKGNGDGEKAYGGKIFTFGSYRLGVHGKGTDIDALCIVPRHVTRNDFFTYFYEELKGDPNIKGVTKIEDAFVPIIKFEFQGIPIDLVFARLNVPVVKDGINLLNDTLLKSMDEKCILSLNGSRVTDEMLNLVPNVKAFHSALRCIKYWAKRRCVYGNPYGYFGGVAFSLCVARVCQLYPNASSFTIVCKFFELFSSWKWPTPVILRPVVDLNYNLKVWDPKVYPSDKYHRMPVITPAYPSMCATHNVSNSTQHVITMEFSRAHEILSNLEKNDFRRIFELSDFFGRYKLFVEVLAMSTLEEGFTKWEGYVESRIRILASKFEAVDDILYAIPFPKAFRVLEESIGKIKPGAEEVKRCTAFFIAIDVIPVKGKKVVVEQQVKEFMEFVKEYEGKESNMWIEINSLKRKDVQGFLRVFYGKENLSKEDENKRRKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD74</accession>
    <name>I7AD74_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF8032 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009265772.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20520835"/>
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        <property type="component" value="Chromosome II"/>
    </dbReference>
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        <property type="entry name" value="DUF8032"/>
    </dbReference>
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        <property type="entry name" value="RE27538P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22949">
        <property type="entry name" value="WHITE COLLAR 2 PROTEIN WC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26087">
        <property type="entry name" value="DUF8032"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8032" evidence="2">
        <location>
            <begin position="133"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8032" evidence="2">
        <location>
            <begin position="247"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="387" mass="45118" checksum="6FF3094B275D33E5" modified="2012-10-03" version="1">MAGERAEYDGIVSGQELIYQFQEDSDDLGEKEYEENEANSLKLRNFVEDEFYQSDGQMQYRGEEEMRLRYGRKKKEKRKKKKNDENKRPSNRKIVNLPASPPYVRFVDGEERLCFKYVTKVSESARDAMPKADLEDNEFCVRFDLDSVDISKLNEKFKADNCVYPRANLPYEMYTGNRWGFETECNRLAWQFVSLNPVLLYGKKGLIQRAVDSYRTINKQSRNKRVVKEDRFPSDIEKRRHVSGAPSTITIQWTNRGILKKCKIQVNIENINYEKIDDEFKSRFSVFMDDFDDTSFGKSKWESHNADNELAVKIAFLNVENTSFWNAIKALDKTTVLKKAIEAYKQKRDEYVSSEDDTEISDVVASTLGGSLGNSKSCNIENFYFDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADQ1</accession>
    <name>I7ADQ1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264197.1">
        <property type="nucleotide sequence ID" value="XM_009265922.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADQ1"/>
    <dbReference type="GeneID" id="20520990"/>
    <dbReference type="KEGG" id="ero:EROM_030790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030790"/>
    <dbReference type="HOGENOM" id="CLU_019279_2_0_1"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd14016">
        <property type="entry name" value="STKc_CK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="304" mass="35501" checksum="7C70988C549DD564" modified="2012-10-03" version="1">MTTEIRNIKLVQKIASGAFGDIFIGQNTTTNQTVAVKLEKKAHYSQLKHEYGVYKALGGTRTPKIYEYGKILYENVYVNGLVMELMGKSLEQLFVTCNRRFSLKTVLMLGEKMVDNVEYLHHRNYVHRDIKPDNFVFDLRGDKLYLIDYGLAKEFRNPMTFKHREMRTDKSLTGTARYASLRTHQGYEQSRRDDLESIGFCMVYFLKGRLPWQGLKAKTKQEKYDRIRENKEGISLYELCMGLPKEIHSFCFYARNLGYEDMPNYAYLRTLLNDALRQRGLRNDGVFDWIARTPSDSLGDLEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADS6</accession>
    <name>I7ADS6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Endoplasmic reticulum membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_031130</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264232.1">
        <property type="nucleotide sequence ID" value="XM_009265957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADS6"/>
    <dbReference type="GeneID" id="20521026"/>
    <dbReference type="KEGG" id="ero:EROM_031130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031130"/>
    <dbReference type="HOGENOM" id="CLU_122466_0_0_1"/>
    <dbReference type="OrthoDB" id="2194158at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="2">
        <location>
            <begin position="12"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="183" mass="21274" checksum="8C4E3DB33FEB965D" modified="2012-10-03" version="1">MKVLLFAIGVLCRRIKLEPKEQFTFRDKVVANQMYKFMYQTPRTSKIELTMFDTKGRQMTKTRDPYSVIYSKIAEDGEIVISVKNNSSKSVKFGYRCPDTSNKAQGGLGPISVDLVSELQNVLETVVQAQRVHIKKHEEHARMVAGSRKWVTRLLIFETLFFIGVLYFLHKDMLKMFENKREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADW6</accession>
    <name>I7ADW6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">HrpA-like helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_040210</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264287.1">
        <property type="nucleotide sequence ID" value="XM_009266012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADW6"/>
    <dbReference type="GeneID" id="20521085"/>
    <dbReference type="KEGG" id="ero:EROM_040210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040210"/>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2130">
        <property type="entry name" value="DEAH helicase family, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042035">
        <property type="entry name" value="DEAH_win-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="18"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="193"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="605" mass="69289" checksum="D494542125B7BD54" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADZ9</accession>
    <name>I7ADZ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">6-phosphogluconolactonase/glucosamine-6-phosphate isomerase/deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264332.1">
        <property type="nucleotide sequence ID" value="XM_009266057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADZ9"/>
    <dbReference type="GeneID" id="20521131"/>
    <dbReference type="KEGG" id="ero:EROM_040670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040670"/>
    <dbReference type="HOGENOM" id="CLU_1391190_0_0_1"/>
    <dbReference type="OrthoDB" id="432544at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="6PGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054:SF0">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine/galactosamine-6-phosphate isomerase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="194" mass="22004" checksum="1DC621FD64DEF612" modified="2012-10-03" version="1">MNVLFTEDFNGEIYKILREYCGKNLALMISGGSLLQCLDDKRYTDLDTSRWKIFYSDEREDQNYLNYTASMGFISKTNGKVYKICTSRPLEEAARMYSTELTDIDVCLLGIGGDGHICSLPPGCKELDSDDYVVALEGNFPVSPKRVSITPRFINKKIKKLYFVVPNSKKKGVHSPDKSITQRITRGFSVILEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE06</accession>
    <name>I7AE06_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_040770</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ELP4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264342.1">
        <property type="nucleotide sequence ID" value="XM_009266067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE06"/>
    <dbReference type="GeneID" id="20521141"/>
    <dbReference type="KEGG" id="ero:EROM_040770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040770"/>
    <dbReference type="HOGENOM" id="CLU_997565_0_0_1"/>
    <dbReference type="OrthoDB" id="289162at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008728">
        <property type="entry name" value="Elongator_complex_protein_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12896">
        <property type="entry name" value="PAX6 NEIGHBOR PROTEIN PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05625">
        <property type="entry name" value="PAXNEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="279" mass="31577" checksum="BB43700BE88EF98E" modified="2012-10-03" version="1">MKSFVRSAGQRGMEKLSTGISGLDAILGECFTRGTSILLLEDENSRIHSTILKVFLSEGIYNQESTLAVVKEGGDVEVYDTGSSMEQENEDRMVIAWRYSKLSLGRPAFKFNLSKKKRFDGILLSGKKATLESILDIAREERELRIAVFSLGSPAWRLSGYEEKKMMRFLFELRRLSRINNHIYMVSAPGFMMPDANFSLYFDTVAEFDSNIFSRFCPNYNGVLEFKKIGGYGCLRINSLESLKYGVKIRKESICVEKVAMPPEDVKPPGNSCIQDKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEC1</accession>
    <name>I7AEC1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_050680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264497.1">
        <property type="nucleotide sequence ID" value="XM_009266222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEC1"/>
    <dbReference type="GeneID" id="20521300"/>
    <dbReference type="KEGG" id="ero:EROM_050680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050680"/>
    <dbReference type="HOGENOM" id="CLU_046346_3_0_1"/>
    <dbReference type="OrthoDB" id="10260596at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014401">
        <property type="entry name" value="Ribosomal_eS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002129">
        <property type="entry name" value="Ribosom_S6_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="217" mass="25280" checksum="3DB5767A634B431D" modified="2012-10-03" version="1">MKLNIAYPTNGTQKIFEIERRNEVKLYDKKIGDQFDGGILGEEFEGAIMEITGGDDYQGFPMVKGHLTKKRVRPLLSKGDAGYRCRRKGVRRRKSVRGSIVSEEICVLNLIMLRPGEKEIDGLTNAVNDVSHLPRKESKLRKMFGVPDEEKNVVGYIRNIFKAECEDPKKIPKIRHNGKRIKKEQERKESIRKMKLERKRILEEERKAYLEKYFNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AER8</accession>
    <name>I7AER8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Translocase of outer mitochondrial membrane40-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_060410</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Tom40 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264632.1">
        <property type="nucleotide sequence ID" value="XM_009266357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AER8"/>
    <dbReference type="GeneID" id="20521438"/>
    <dbReference type="KEGG" id="ero:EROM_060410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060410"/>
    <dbReference type="HOGENOM" id="CLU_086429_0_0_1"/>
    <dbReference type="OrthoDB" id="19656at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="entry name" value="Porin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027246">
        <property type="entry name" value="Porin_Euk/Tom40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037930">
        <property type="entry name" value="Tom40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10802">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01459">
        <property type="entry name" value="Porin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="284" mass="31335" checksum="9983558D0298B029" modified="2012-10-03" version="1">MRWISNILRDIRRSCSEREITSTFDTYNAEANEIVTSQTCSGVRTEISKTIFPFLQVSRVDILDRNAHVSQMFGTLSLKNSLVQVSMDHENSIGVRAMNMYGNLATRIHTIITGKKEVFSQVEMEMKNRISSFGLKMISPAIKSTGMVYVANTLQQFGSLSLGAEIIGADTEVGISMCGRYEHRNGVFSVGLQQFTALSLSYYHKIGKFFDIGAEIQAPRANPPSAAIGCRLSTSKTQVKANVNSDMSLGFYLGERLTEGLTFNVNAEVNKSGCSHGFGFSLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF73</accession>
    <name>I7AF73_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase II subunit A C-terminal domain phosphatase</fullName>
            <ecNumber evidence="6">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_070840</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">This promotes the activity of RNA polymerase II.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="6"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264832.1">
        <property type="nucleotide sequence ID" value="XM_009266557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF73"/>
    <dbReference type="GeneID" id="20521644"/>
    <dbReference type="KEGG" id="ero:EROM_070840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070840"/>
    <dbReference type="HOGENOM" id="CLU_007683_2_0_1"/>
    <dbReference type="OrthoDB" id="10249888at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039189">
        <property type="entry name" value="Fcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011947">
        <property type="entry name" value="FCP1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02250">
        <property type="entry name" value="FCP1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081">
        <property type="entry name" value="RNA POLYMERASE II CTD PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23081:SF36">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="8">
        <location>
            <begin position="56"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="7">
        <location>
            <begin position="306"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="408" mass="47736" checksum="3794F80CC835B7D6" modified="2012-10-03" version="1">MDECDHPIRLGTLCGVCGMEIQKDSHLFCALYNTDNVKITHKEAVSIYKEKIKTLEMQMKLILVLDLDQTVLHTAYGASSEKGIVRFTMDGCKYSVKLRPNLKRMLRKVSRLYEIHVYTMGTRPYAERIVRIIDPTRKYFHDRIITRDENQGVLVKRLSRLFPYNHKNIVILDDRADVWDYCENLVLIKPFWYFNRVDINDPLRLKRKIEKESEECKELGDSVRKRKKVEEVEDTQVISKLDGMALEYSSGESSVQEDAKGVGERKTKEGFEGDNELVRMARFLRKVHRKYFSSKHRNVKKVLRRIRKKIFDGDRFYVVGPTNRVWLMKVIEMHGGIISGLGNEVDFIVSSCLEEARELAQKIECVVVSPKWISDCVYSLKRVGHGRYVICDCRAKDEYEEELEKLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFA0</accession>
    <name>I7AFA0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264867.1">
        <property type="nucleotide sequence ID" value="XM_009266592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFA0"/>
    <dbReference type="GeneID" id="20521679"/>
    <dbReference type="KEGG" id="ero:EROM_071190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071190"/>
    <dbReference type="HOGENOM" id="CLU_1343228_0_0_1"/>
    <dbReference type="OrthoDB" id="2191374at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="216" mass="24370" checksum="654214639752C93A" modified="2012-10-03" version="1">MYPYKQKCQTSPMVVRTSQGHSSRKVFDIRDGTSLGNLLRDYARAFKLYHIDMENVRESIVPVLTYAGLLTIQIFGFLCIVSWAEDMGGISSTLGLSFGAFCAMIMFFFGAGYIYRGTLGKGTDTSYISYVYVVALSVVHYPYLMFVGLLTKGIWMFLCMMAITSISQFLIRTSMLRNHSFENHRDGFMFDIITFILQCGFCFGSLSLFSLLSPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFB1</accession>
    <name>I7AFB1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L22/L17e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_071340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264882.1">
        <property type="nucleotide sequence ID" value="XM_009266607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFB1"/>
    <dbReference type="GeneID" id="20521694"/>
    <dbReference type="KEGG" id="ero:EROM_071340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071340"/>
    <dbReference type="HOGENOM" id="CLU_083987_0_1_1"/>
    <dbReference type="OrthoDB" id="10254664at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="183" mass="20771" checksum="036699FB829F953E" modified="2012-10-03" version="1">MGIKYSYEVHDPSKAVKGKIDNAKVSFKKTRETCSTLRKRKVVDAIEYLKNVINKTECVPFRRYNRGCGQTHQARKFSIPKREIVKANGEKVIKRGHVQTRGRWPKKSAEFVITLLENLKESAETKNLDVENLVITHIQVSKAPKIFGRTFRAHGRVNPFNKSPCHIQAVAEVVGTNDVSIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFF1</accession>
    <name>I7AFF1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proliferating cell nuclear antigen</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264932.1">
        <property type="nucleotide sequence ID" value="XM_009266657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFF1"/>
    <dbReference type="GeneID" id="20521748"/>
    <dbReference type="KEGG" id="ero:EROM_080120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080120"/>
    <dbReference type="HOGENOM" id="CLU_102260_0_0_1"/>
    <dbReference type="OrthoDB" id="2191577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25697" checksum="22160AA5C940DC6D" modified="2012-10-03" version="1">MDCVMSSQNAFTVDKILDAVGASTMLIDTTNGMVFHLVTHDRTLVLDFNLEESFFKSLSLKNKCILIPKQKFYIKKIKELKIITNEYVMVFEYVFDKYTLRRRVFYSQSTRFVIDFKIDCTGEINSSAMGLALKEIGDHFATLRVINNTGEICSEGTIIKFPLTLPTDFSVKVIGGNLKSIFEVSDLFIKMIMSYGPTNLSINFVLLGNGTKASFFTAINHNRTSKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG18</accession>
    <name>I7AG18_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">GPI-anchor biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_090580</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit in the complex catalyzing the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265172.1">
        <property type="nucleotide sequence ID" value="XM_009266897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG18"/>
    <dbReference type="GeneID" id="20564282"/>
    <dbReference type="KEGG" id="ero:EROM_090580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090580"/>
    <dbReference type="HOGENOM" id="CLU_009583_19_0_1"/>
    <dbReference type="OrthoDB" id="734129at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017176">
        <property type="term" value="F:phosphatidylinositol N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013234">
        <property type="entry name" value="PIGA_GPI_anchor_biosynthesis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL BIOSYNTHETIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45871:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08288">
        <property type="entry name" value="PIGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="PIGA GPI anchor biosynthesis" evidence="5">
        <location>
            <begin position="43"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="4">
        <location>
            <begin position="191"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="409" mass="46572" checksum="EE65625F84C63CCB" modified="2012-10-03" version="1">MKRLNIAMVSDFFYPSTGGIETHIRYLSEELISMGHKVIVITHKTGDLVGCRMVGTIKVYFLDIPVLCRNTTFPSLYSNLPLFKEIFDSEKIEIVHGHQTMSNMCIEGLFHARTLNLKTVITDHSIFEVGPFENIVVNALCRLALKNIDRCICVSYTSKENTHIRTMIPLEKIHVIPNAIVSDIFCPREEGKSDEKTVVVASRLMFRKGIDLLIGAIPLICKGDPTVRIIIVGDGPMKEGIEQVLDENELYDRVEIMHEVDHEKVGEIMRRGDVFLNTSLTETFCIAIVEAASCGLHVVSTNVGGIHEVLPPDMITFSKITCEDLAEKVLMALRKSNHNPQDYNKRLKNIYNWKRVARMTEKVYMDIEQTFLGYRNRRELYKGATGILSRLMITIEYLFLFLLGLRNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG67</accession>
    <name>I7AG67_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265242.1">
        <property type="nucleotide sequence ID" value="XM_009266967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG67"/>
    <dbReference type="GeneID" id="20564353"/>
    <dbReference type="KEGG" id="ero:EROM_091290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091290"/>
    <dbReference type="HOGENOM" id="CLU_1610741_0_0_1"/>
    <dbReference type="OrthoDB" id="2195633at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="169" mass="19869" checksum="D9276804A0E3AEA6" modified="2012-10-03" version="1">MTSAEDRELGLEKKIHKAALLETVDVLNKVADKRDKGGRKKAYIKSEEKENKSKKDVQRKADRHGIKPSKQKRNAMKKCGQTTDEKEDKDLKKCPMKMTRKERRLEKLKNKGKGPSSKEYYEKFIKKYKDKSPEKKVSKDILPSKRKEDFPKDDDSKRKKTPFSQKKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGS9</accession>
    <name>I7AGS9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exportin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_110110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265492.1">
        <property type="nucleotide sequence ID" value="XM_009267217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGS9"/>
    <dbReference type="GeneID" id="20564610"/>
    <dbReference type="KEGG" id="ero:EROM_110110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110110"/>
    <dbReference type="HOGENOM" id="CLU_011906_0_0_1"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="34"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1024" mass="119977" checksum="49A9741B1DF150F6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGW9</accession>
    <name>I7AGW9_ENCRO</name>
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        <submittedName>
            <fullName evidence="13">Exonuclease 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_110630</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009267267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGW9"/>
    <dbReference type="GeneID" id="20564662"/>
    <dbReference type="KEGG" id="ero:EROM_110630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110630"/>
    <dbReference type="HOGENOM" id="CLU_008978_3_0_1"/>
    <dbReference type="OrthoDB" id="26491at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09901">
        <property type="entry name" value="H3TH_FEN1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
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    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="11">
        <location>
            <begin position="138"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN84045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="366" mass="42861" checksum="DD8E5422F2F99A54" modified="2012-10-03" version="1">MGISGLLPIVATKLVKRHISSYRHQRIGIDGHAWLYQILSFVAEEMFFKVPTRKHITMFEEKVKVLESYGITPIIVLDGDTLSSKEETNRKRYIKKDKNRKEAEHWLVKNDPEKAKGFMRQCIAVTKEIVSDIARMLERINVEYIISPYESDAQLCYLERIGYIDCILTEDSDLIPYGSNRILYKFDNTFVQEFTRGCLSEARGRDFEENILDISILSGCDYLASIQGVGVVTAHKLLSKEKTVEKVVEYLQHRKAIPDNYLDDFFKAKKTFLHQIVYDPIQQKRRYLRDVKEKLDFLGTLKEEEYRIEDGPLELFFKSSRRTRAIKRHFIPVARKRRSEASEIKKSKREGCSSKMDIHIHSPYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH04</accession>
    <name>I7AH04_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Orm1-like membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265582.1">
        <property type="nucleotide sequence ID" value="XM_009267307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH04"/>
    <dbReference type="GeneID" id="20564702"/>
    <dbReference type="KEGG" id="ero:EROM_111030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111030"/>
    <dbReference type="HOGENOM" id="CLU_072117_4_0_1"/>
    <dbReference type="OrthoDB" id="1932233at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007203">
        <property type="entry name" value="ORMDL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12665">
        <property type="entry name" value="ORMDL PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04061">
        <property type="entry name" value="ORMDL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="147" mass="17389" checksum="4BF32034AAE7C12E" modified="2012-10-03" version="1">MGIDRERKVDVSQNVAWTLQRGSWIIHVLVIFLLKMTLSVVFSDSIGWQLSLIIYNISTFIFFHMIPGDPFNDRYSLYTFWEQLSEQLEGTPDLIFIALFPLIAFTIINFIVKWNTFLFWLCIASLFIVTIPKFGFMHLRRILIFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AHA9</accession>
    <name>I7AHA9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_111940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265672.1">
        <property type="nucleotide sequence ID" value="XM_009267397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AHA9"/>
    <dbReference type="GeneID" id="20564793"/>
    <dbReference type="KEGG" id="ero:EROM_111940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111940"/>
    <dbReference type="HOGENOM" id="CLU_1427971_0_0_1"/>
    <dbReference type="OrthoDB" id="2192326at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="190" mass="22219" checksum="72062A5862258505" modified="2012-10-03" version="1">MKTKIKTFIIDNFKYKISKDQVIKMIKSESVSIKTYNGFVFKILLKYKYSFGISGEPRILSTNTFLDKEFLFEDLFLVNGRKQEGILRGEEILFEKQGIPTEYYTSTMHLSYRFLGRMIAKDEVKERFRECSIGLGLSNDVEKECAEHLKKGLQIYIQKIVNRCNRKITITGLKAGLEKHIRLFNGLDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL42</accession>
    <name>I7AL42_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Actin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_010240</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263866.1">
        <property type="nucleotide sequence ID" value="XM_009265591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL42"/>
    <dbReference type="SMR" id="I7AL42"/>
    <dbReference type="GeneID" id="20520651"/>
    <dbReference type="KEGG" id="ero:EROM_010240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010240"/>
    <dbReference type="HOGENOM" id="CLU_027965_0_2_1"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000218">
        <property type="entry name" value="actin, alpha sarcomeric/skeletal-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000291">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000047">
        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000058">
        <property type="entry name" value="Putative actin-related protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004001">
        <property type="entry name" value="Actin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00406">
        <property type="entry name" value="ACTINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="375" mass="41919" checksum="DC6469E5243BDC36" modified="2012-10-03" version="1">MSEIVQALVIDIGSGVVKSGFAGDDAPRAVFPSIVGSPKHKGVMVGMGQKDAYVGDEAQTKRGILHIKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKITQIMFETFNVPSFYISIQAVLSLYASGRTTGIVFDSGDGVSHVVPIYEGYSLPYAINRIDLAGRDLTDYLQLILTESGNSFTTTAEREIVRDIKEKLCYVSLNYEEDMRNTEHLASITKTYEMPDGQVISIGNERFRAPELLFQPKLRGLELKGVHQNIYDSIMKCDVDIRKELYGNIVLSGGTTMYPGLAERILNEIKALAPPVIKIGVVAPPERKYSVWIGGSILASLSTFQQMWVSKAEYQEHGPSIVHRKCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALE1</accession>
    <name>I7ALE1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">isopentenyl-diphosphate Delta-isomerase</fullName>
            <ecNumber evidence="3">5.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_020140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:23285"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from isopentenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IPP isomerase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="3"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263986.1">
        <property type="nucleotide sequence ID" value="XM_009265711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALE1"/>
    <dbReference type="GeneID" id="20520772"/>
    <dbReference type="KEGG" id="ero:EROM_020140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020140"/>
    <dbReference type="HOGENOM" id="CLU_060552_0_1_1"/>
    <dbReference type="OrthoDB" id="510307at2759"/>
    <dbReference type="UniPathway" id="UPA00059">
        <property type="reaction ID" value="UER00104"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050992">
        <property type="term" value="P:dimethylallyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009240">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02885">
        <property type="entry name" value="NUDIX_IPP_Isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011876">
        <property type="entry name" value="IsopentenylPP_isomerase_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02150">
        <property type="entry name" value="IPP_isom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10885:SF0">
        <property type="entry name" value="ISOPENTENYL-DIPHOSPHATE DELTA-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018427">
        <property type="entry name" value="Isopntndiph_ism"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="7">
        <location>
            <begin position="33"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="225" mass="25870" checksum="61D433DC77E0F908" modified="2012-10-03" version="1">MDNYNRNILLVNDKDKVVGIGKALRTHFREHLMLHRAFSVFLFNSRNELLIQKRASGKLLFPNKWTNSVCSHPFVNDLSFINPLLDVKIHAVKRIDHELGIGSILIDDLRFVSRIIYKASPTERYSRILPGIPTTQKISTFPDPNNLSGSYSSDDFHEWEVDYIILAVSDASPCPNPNEVSEIRYVCKEELQELIARDLASPWLDEISKHVDIFDIKQSYFPSDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALK2</accession>
    <name>I7ALK2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_020790</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264051.1">
        <property type="nucleotide sequence ID" value="XM_009265776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALK2"/>
    <dbReference type="GeneID" id="20520839"/>
    <dbReference type="KEGG" id="ero:EROM_020790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020790"/>
    <dbReference type="HOGENOM" id="CLU_080597_1_0_1"/>
    <dbReference type="OrthoDB" id="1703270at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="173" mass="19385" checksum="FA6E3463A11441FE" modified="2012-10-03" version="1">MGINHRGDHKRRKTGAKAVISRKKRNNRAGSQPSATKIGERRVMPVRVRGGNRKHKALRLDVGHFRFITTGKIRPAKLLQVVYHPSSNDLVRTNTLTKSSVVKISAEGFKEDIKAVASNVDPELHESFEKGYLYAIITSRPGQIGAATGHVLQGDELKFYSDKFNKKTKEVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM50</accession>
    <name>I7AM50_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_031370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264256.1">
        <property type="nucleotide sequence ID" value="XM_009265981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM50"/>
    <dbReference type="GeneID" id="20521051"/>
    <dbReference type="KEGG" id="ero:EROM_031370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031370"/>
    <dbReference type="HOGENOM" id="CLU_080024_2_0_1"/>
    <dbReference type="OrthoDB" id="6353017at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="4">
        <location>
            <begin position="18"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="200" mass="22619" checksum="BCAD988ECAEFE909" modified="2012-10-03" version="1">MHITSAMKHVKLPPSAISRRKEPKSKNIYLRALSDFYTKVADNTSVLAIKKISKRLKMSKSDRQPVKISKIVSELENSEGKVAVVVAKVLDDDRIMEIPAIKVIALQWSKGVKEKIERYGGSIGTLDQLFELCPNMDDIKLISTDKFARKSAKFWGPAPGEKGSTTYPRANQKCHNREKRIMMKGRKTQHLKKQVLNKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMB5</accession>
    <name>I7AMB5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264336.1">
        <property type="nucleotide sequence ID" value="XM_009266061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMB5"/>
    <dbReference type="GeneID" id="20521135"/>
    <dbReference type="KEGG" id="ero:EROM_040710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040710"/>
    <dbReference type="HOGENOM" id="CLU_799327_0_0_1"/>
    <dbReference type="OrthoDB" id="2195260at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="347" mass="40571" checksum="0B80C0C5F1366BCF" modified="2012-10-03" version="1">MGARTQSTATEKLVVSKAVDIYGVSDEASVTKYIATMYGYIHPKRWKEIYQEVLETYKKNGYSSPVDFCKDLRFDIARKLYMEKMALRNKLLEGEDVEYSGQYISYDWNIGSIKKIDDIARATEDALDNRQLYPSTDPRIIRQHIEAMSKWRDRAQGNLDGASRMSESKKRHHIVVDKEKRRSYHRMSDEERELDDFLDRMEEEHYIMGQEGINPNRLRGKYPPLKTSLMGSRFSGYRDVDGPSASLENQYRVIVNRIAGGVMHESNGEAWRKDLRVIVQYHMKRIGMVNELEKVNVLIDSSSMAHILMNILMLLQGMIDRPRNRDLVPDYMSFKKDLLMFGNHYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMK4</accession>
    <name>I7AMK4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264441.1">
        <property type="nucleotide sequence ID" value="XM_009266166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMK4"/>
    <dbReference type="GeneID" id="20521243"/>
    <dbReference type="KEGG" id="ero:EROM_050110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050110"/>
    <dbReference type="HOGENOM" id="CLU_860597_0_0_1"/>
    <dbReference type="OrthoDB" id="2195291at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="323" mass="35358" checksum="5953D9D8E6841967" modified="2012-10-03" version="1">MSKGKIIQLSIVTLLGAAITILALYMFSEGPTGTLTHDDIKSLSAENPFRFKDSGENGENTVTEVKFFTCLNKILKSLKSADNNLIRVLCCNEILEKEAEEKGNGEREAHVRNAIIEARNLMFGVSNSKETIEKILKKGNEKSGEELIEAIPSAFFEALSAEQPHKQAKGEELNTALANLFIWLWLNGSLPEDKNTQTFRKTYADNQSVQNLLKSLDEETKGVANSKISVTIENKEISMSVIEHILTSIFQASMRMGSGIPQVSLPVKMAIISAGAGLEKEKPKDINQGKGIIEFLFGSNEDKSKNETSADEAKSTTDADGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMN6</accession>
    <name>I7AMN6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_050560</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UTP23/FCF1 family. FCF1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264486.1">
        <property type="nucleotide sequence ID" value="XM_009266211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMN6"/>
    <dbReference type="GeneID" id="20521288"/>
    <dbReference type="KEGG" id="ero:EROM_050560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050560"/>
    <dbReference type="HOGENOM" id="CLU_081098_1_0_1"/>
    <dbReference type="OrthoDB" id="76105at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09864">
        <property type="entry name" value="PIN_Fcf1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037503">
        <property type="entry name" value="Fcf1_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="PIN" evidence="6">
        <location>
            <begin position="52"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="179" mass="20666" checksum="66222B403B1A50FC" modified="2012-10-03" version="1">MGVKHAIKIKKVANIWDRRNPRVEKPKNIPSINENPRNTEKFLLSHTLRPPYNVILDTNFINDCIRKKYDIREQLMKVVNASIKICVPECVIAELEKLGRPFRVALALVRDNDVQRLECDHKGTYADDCIFNRVSAHRCYIVATSDTALRQRIKAIPGVPLITYRGQRCFIERFIPAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMR4</accession>
    <name>I7AMR4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_050980</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264526.1">
        <property type="nucleotide sequence ID" value="XM_009266251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMR4"/>
    <dbReference type="GeneID" id="20521330"/>
    <dbReference type="KEGG" id="ero:EROM_050980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050980"/>
    <dbReference type="HOGENOM" id="CLU_055194_0_2_1"/>
    <dbReference type="OrthoDB" id="2877at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039769">
        <property type="entry name" value="Bud23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022238">
        <property type="entry name" value="Bud23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734:SF0">
        <property type="entry name" value="18S RRNA (GUANINE-N(7))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12589">
        <property type="entry name" value="WBS_methylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="10">
        <location>
            <begin position="52"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal" evidence="11">
        <location>
            <begin position="204"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="248" mass="27700" checksum="9C687F2433A9439A" modified="2012-10-03" version="1">MGIPELSGPAEVYYDEETSLKYTQNSRIVYIQRELTERCLELLEPKDGGLLLDVGCGSGLSGSVLAENGHPWIGIDISMDMLKLGAAKKEGVGYIRMDMGRGLEFQPGTFDGVVSVSAIQWLFHSYSSDDHPVRRLRTFFTALYSICKPDARCVLQFYLKSQGQIEMMKAEAIRAGFSGGIQVDNEGTRNVKSFLVLTSVKGPGKKRGKKKKGMGRIDAILRRKEKLRLRGVEVPKDTKYTGRKRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMW1</accession>
    <name>I7AMW1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_051540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264581.1">
        <property type="nucleotide sequence ID" value="XM_009266306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMW1"/>
    <dbReference type="GeneID" id="20521386"/>
    <dbReference type="KEGG" id="ero:EROM_051540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051540"/>
    <dbReference type="HOGENOM" id="CLU_1214747_0_0_1"/>
    <dbReference type="OrthoDB" id="2191354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="228" mass="26218" checksum="C359FD412A5A071F" modified="2012-10-03" version="1">MGMQEKTLLLVVGNKEMSMLSGTSQAYVLSSDKGYGVKRVSPSNTFIVKRGNKYIKIDYVLELVENPLDLEKIYHLIPPSSIWNLLPPVDLKSHFYLGDGQVRLVEKELKLLKLNDGHVRISYKDMADIVCYMSSIRDRDDFDLKMDIYPHLVKEWALENFTGDSTEIGLYCLLGCDEENDMTSFLKRWEDSSPNEINIEGLVRQVNSTFIIQEKKARLQQYLNKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMY1</accession>
    <name>I7AMY1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nucleolar transformer 2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_060140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264606.1">
        <property type="nucleotide sequence ID" value="XM_009266331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMY1"/>
    <dbReference type="GeneID" id="20521411"/>
    <dbReference type="KEGG" id="ero:EROM_060140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060140"/>
    <dbReference type="HOGENOM" id="CLU_1288886_0_0_1"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF119">
        <property type="entry name" value="NUCLEAR RNA-BINDING PROTEIN SART-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="136"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="217" mass="25087" checksum="539F695D45322143" modified="2012-10-03" version="1">MEREEKEGKRDVRSKWKGDYSGGNRSQCGKPFYSDRHDHYNEYDGIRRRKINQHQGNFIRYRENGNYLGRTSHAPHSRTWTPRGPYPSNTYETREGRSYGIERSLRGGEEYERPGGHSYRNEKYGLKAKRNGSPSKAIGVFGLGAYITEDDVKNFLKERISGITNYKVVIVYDKYSGLSKGYGFIQFDTLDDAITAKNKLLGQTIKGKEIRIDYSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN29</accession>
    <name>I7AN29_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="6">dUTPase</shortName>
            <ecNumber evidence="6">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_060350</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in nucleotide metabolism via production of dUMP, the immediate precursor of thymidine nucleotides, and decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>dUTP + H2O = dUMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the dUTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="6"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264626.1">
        <property type="nucleotide sequence ID" value="XM_009266351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN29"/>
    <dbReference type="GeneID" id="20521432"/>
    <dbReference type="KEGG" id="ero:EROM_060350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060350"/>
    <dbReference type="HOGENOM" id="CLU_068508_3_0_1"/>
    <dbReference type="OrthoDB" id="10261072at2759"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046081">
        <property type="term" value="P:dUTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008181">
        <property type="entry name" value="dUTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00576">
        <property type="entry name" value="dut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001862">
        <property type="entry name" value="PRK00601.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11241:SF0">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="dUTPase-like" evidence="7">
        <location>
            <begin position="14"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="144" mass="15889" checksum="C2BA314DA7BCA2C5" modified="2012-10-03" version="1">MTVEEIRVYKVHSNAKIPKRQSEGAAGYDVHSVESGVILPGERKSIRTGLIWDIPQSIVGLVFGRSGLALKNWMEVVETCVYPGENKELVITLINNGKQPFEYEECCRIAQLVFVPVLSCEVNLVESLDSTERGCLGFGSTGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN75</accession>
    <name>I7AN75_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264681.1">
        <property type="nucleotide sequence ID" value="XM_009266406.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN75"/>
    <dbReference type="GeneID" id="20521488"/>
    <dbReference type="KEGG" id="ero:EROM_060920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060920"/>
    <dbReference type="HOGENOM" id="CLU_933926_0_0_1"/>
    <dbReference type="OrthoDB" id="2191879at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="298" mass="33526" checksum="A34B90FB008EA22D" modified="2012-10-03" version="1">MDMKQDLFTYRHPGSIQAFGLNTDTLLVATSSILVEWDRVRRRIVKEIRMDEEIVDIKCFSGYWVLITKSDIGFLDGNKTKRIGSKGSLDVSIVYKDWLLTVGNEKLVIISIRKGNAEIRKVRLISTNGMCTCGVAATDGLFLSFENGKVFSIKESEVMNIVLGKINEVVMDKFSCLIFLKEAIVSISTLGKELVISLFNKKVLVLDLDTKDVSYTELPHNIRGSTVWNDLIVVSDCKNNILFLDRRLRITYSNCLKEEICGVLADRRGLAIGFRVGVVKEYVGESRTRPGVIRGMVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANE4</accession>
    <name>I7ANE4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Rad18-like recombination and DNA repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_070330</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family. SMC6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264781.1">
        <property type="nucleotide sequence ID" value="XM_009266506.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANE4"/>
    <dbReference type="GeneID" id="20521592"/>
    <dbReference type="KEGG" id="ero:EROM_070330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070330"/>
    <dbReference type="HOGENOM" id="CLU_009063_0_0_1"/>
    <dbReference type="OrthoDB" id="10072614at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19306:SF6">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad50/SbcC-type AAA" evidence="14">
        <location>
            <begin position="12"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="SMC hinge" evidence="13">
        <location>
            <begin position="437"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="188"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="663"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="801"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="980" mass="115301" checksum="57C6DC05457C8E11" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANF9</accession>
    <name>I7ANF9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264801.1">
        <property type="nucleotide sequence ID" value="XM_009266526.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANF9"/>
    <dbReference type="GeneID" id="20521612"/>
    <dbReference type="KEGG" id="ero:EROM_070530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070530"/>
    <dbReference type="HOGENOM" id="CLU_980145_0_0_1"/>
    <dbReference type="OrthoDB" id="2194467at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="286" mass="32865" checksum="DA6DAAC3403FAA7F" modified="2012-10-03" version="1">MKGEVEFLESDSPGRRPCTYTRSQAMALYEEHRKHILKELIAREVQKRITNKILGKRETEEGLLFIKHRHDTSMDGISGMLTDGEYSISLEEKGVCDEDRTQYSKTPHSPPSSLDTGRCKPNANLRRPERFVDVEACVSGDEGNSLDADEGDPDDLSFIASSDEDYEAPTKKHNRDMLKINKRILRNLKKKFMKKAQSKAFFGPEGHTEKEPSDCEVIEEEDIDVSEDTVEDIVEDFMFSKEEMEYPHEAKPFFTKPGQIEFTENIKLAEKRLGERKSGWDFDESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANJ7</accession>
    <name>I7ANJ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264846.1">
        <property type="nucleotide sequence ID" value="XM_009266571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANJ7"/>
    <dbReference type="GeneID" id="20521658"/>
    <dbReference type="KEGG" id="ero:EROM_070980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070980"/>
    <dbReference type="HOGENOM" id="CLU_657374_0_0_1"/>
    <dbReference type="OrthoDB" id="2191304at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="112"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="154"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="401" mass="44728" checksum="31F9B1CE2CF0AD7C" modified="2012-10-03" version="1">MNEEYEMSEKDYKKMRKSQPKRERILREDFGDPSEIGGFKGPWSRFVGAREDKKGPRDVKEEQSLRRIVNKHCLTVGKERSRSNYFLSMEDASLSRVPSKRFLVPKGNIMLFHDHKDMVSSVSLFRRHKLLLTSGLDGKAYLYNLRAGDLVTTYMGHSKGVSSATLSECESKFSTISFDGFLKVWDIETGRCNAKIDLECPLTCQSFKELGKAVFIGGLDGRIRTVDTRNRKALNGAKEESHPSRQTTFGPSSICDILLVEQDNFLVFTRKKGSLHYLDLRNDRIVKTHNGSCSFIGLNPEENTLMITASDEVILLDVSTLEEGGERIKALGCSTRVKASPDGSMLCFGDTNGLVNFIARSSTKETSLGSTSEALTTIDWIDGSSSSLVSGDVLGNIKVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANQ8</accession>
    <name>I7ANQ8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_071680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="1"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264916.1">
        <property type="nucleotide sequence ID" value="XM_009266641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANQ8"/>
    <dbReference type="GeneID" id="20521732"/>
    <dbReference type="KEGG" id="ero:EROM_071680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071680"/>
    <dbReference type="HOGENOM" id="CLU_072095_3_0_1"/>
    <dbReference type="OrthoDB" id="10039976at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="63"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="205" mass="23309" checksum="C4E6537FC3619957" modified="2012-10-03" version="1">MKRKNQILLTIGTIATLTTLLFLGDSFFNSRGSCACKHDKCTSNIDSKNLNMRGIPIIKRDKYILRGLDIGDYNKGFIDCINEVTEPGVVTQKQFEERYLSLCKDGCYKIVVAYDPKDEKVIGSGTLFIEKKFIRGCAAKGHIEDVVVLKEKRGKGIGKDILETLIWISKKMGCYKTALVCDLKNIEFYKKCGLKEKEREMVMYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANS6</accession>
    <name>I7ANS6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vacuolar H+-ATPase V1 sector subunit H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase H subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264936.1">
        <property type="nucleotide sequence ID" value="XM_009266661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANS6"/>
    <dbReference type="GeneID" id="20521753"/>
    <dbReference type="KEGG" id="ero:EROM_080170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080170"/>
    <dbReference type="HOGENOM" id="CLU_713772_0_0_1"/>
    <dbReference type="OrthoDB" id="10263554at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.150">
        <property type="entry name" value="V-type ATPase, subunit H, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004908">
        <property type="entry name" value="ATPase_V1-cplx_hsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011987">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038497">
        <property type="entry name" value="ATPase_V1-cplx_hsu_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10698:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11698">
        <property type="entry name" value="V-ATPase_H_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03224">
        <property type="entry name" value="V-ATPase_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase V1 complex subunit H C-terminal" evidence="5">
        <location>
            <begin position="265"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF11698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="375" mass="43508" checksum="ECEEBBB63910577A" modified="2012-10-03" version="1">MFLERLEAKTRDSLPPEVISKSVDRFKEIKSHGDRKEVSAFLGKENSGASILLSHPDLLDEDELIDLLFCSDIYSRYKSAEMLTKLYENKSHRKEYFSFFKQMLKDSTSYDESNYLLSLLIDFLSCKSNSNFDVEKRKVALVTNKMFMEILHTSVFVKEVQYNTLMVILIFSYSTECVGKMGTLIDDVVIIVKEKAREKILRVCYGIIANVLESGYVFSPGRLNDISKCTDLLLEGGYGDEELEQDLKKVRARIAQNAKRFCLRNYLNELFSGRFEDSEYHHKEDFWNNNLDMLMKNKVEIVKVLKKYLKSSNPSWVCLACSDIFQLVKVLPEINAVLNKYQVKEVLFNLINSDNDDIRFHAIQTLYTCISSEWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANV1</accession>
    <name>I7ANV1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sm protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_080470</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the snRNP SmB/SmN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264966.1">
        <property type="nucleotide sequence ID" value="XM_009266691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANV1"/>
    <dbReference type="GeneID" id="20521783"/>
    <dbReference type="KEGG" id="ero:EROM_080470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080470"/>
    <dbReference type="HOGENOM" id="CLU_076902_4_2_1"/>
    <dbReference type="OrthoDB" id="2020720at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071004">
        <property type="term" value="C:U2-type prespliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070990">
        <property type="term" value="F:snRNP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050914">
        <property type="entry name" value="snRNP_SmB/NAA38-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10701">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN-ASSOCIATED PROTEIN B AND N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="79" mass="9198" checksum="8BF005409E2AF798" modified="2012-10-03" version="1">MKSNLEKYVNYRVKARMRDGRWMEGTMLAVDGDTNAVLDDTEEFRKIKGSKEARRRILGLVVIRGDFIMDIEIISKPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANW3</accession>
    <name>I7ANW3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">WD40 domain-containing putative poly(A)+ RNA export protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264981.1">
        <property type="nucleotide sequence ID" value="XM_009266706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANW3"/>
    <dbReference type="GeneID" id="20521801"/>
    <dbReference type="KEGG" id="ero:EROM_080650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080650"/>
    <dbReference type="HOGENOM" id="CLU_937204_0_0_1"/>
    <dbReference type="OrthoDB" id="10262475at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="288" mass="31854" checksum="5C943AC3A71ABF05" modified="2012-10-03" version="1">MLHELDFISQETITSISYNKDLLVSSWSGEVMLYSGVTFEARGSVEAETPITKAMLTPEAVIAGDIEGNVRIYDRRLNEISRILSGVGGIQLLHEYRGCVVAGGWNKKIAFINGCVENVMSTEKKVYCSDLIGDILLVGQQEHVVAYDLRTNASFFNRKFNMVVRSLVLTGKGFFAGTTDGRIYYEDFGDGSKSYVFNAHYSISGDSKVFYPVNSLRMGKYLLSGGSDGRVLRWKLGVKKTYKEIACDRTGVSSLCVAGERVAVGFSYSYDRGECNTAHRSRVVVVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANZ9</accession>
    <name>I7ANZ9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_081055</name>
        <name evidence="7" type="ordered locus">EROM_081080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265018.1">
        <property type="nucleotide sequence ID" value="XM_009266743.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265021.1">
        <property type="nucleotide sequence ID" value="XM_009266746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANZ9"/>
    <dbReference type="GeneID" id="20521840"/>
    <dbReference type="GeneID" id="20521843"/>
    <dbReference type="KEGG" id="ero:EROM_081055"/>
    <dbReference type="KEGG" id="ero:EROM_081080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081055"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081080"/>
    <dbReference type="HOGENOM" id="CLU_129470_3_0_1"/>
    <dbReference type="OrthoDB" id="10263513at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004977">
        <property type="entry name" value="Ribosomal_eS25"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12850">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03297">
        <property type="entry name" value="Ribosomal_S25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU366057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="109" mass="12284" checksum="AFC0702B617DECB9" modified="2012-10-03" version="1">MAKKIQESKEKKALKAMSGTRKDKKKWGDGKKKEEVHKIVTVSEDLLSKVRKDVGRASVVTRYMVGSRHNLNLGVAENILRHLSEEGVIERVRGNSRMTMYAGCASQEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7APX5</accession>
    <name>I7APX5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_101140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265426.1">
        <property type="nucleotide sequence ID" value="XM_009267151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APX5"/>
    <dbReference type="GeneID" id="20564544"/>
    <dbReference type="KEGG" id="ero:EROM_101140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101140"/>
    <dbReference type="HOGENOM" id="CLU_328731_0_0_1"/>
    <dbReference type="OrthoDB" id="5632at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051144">
        <property type="entry name" value="Formin_homology_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733">
        <property type="entry name" value="FORMIN-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45733:SF31">
        <property type="entry name" value="GENOME ASSEMBLY, CHROMOSOME: II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="2">
        <location>
            <begin position="510"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="876" mass="101374" checksum="732B4548E392573C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ15</accession>
    <name>I7AQ15_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="16">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_101540</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265466.1">
        <property type="nucleotide sequence ID" value="XM_009267191.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ15"/>
    <dbReference type="GeneID" id="20564584"/>
    <dbReference type="KEGG" id="ero:EROM_101540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101540"/>
    <dbReference type="HOGENOM" id="CLU_001666_4_2_1"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21407">
        <property type="entry name" value="1B_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18039">
        <property type="entry name" value="DEXXQc_UPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040812">
        <property type="entry name" value="UPF1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018999">
        <property type="entry name" value="UPF1_CH/ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF364">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09416">
        <property type="entry name" value="UPF1_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51997">
        <property type="entry name" value="UPF1_CH_RICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Upf1" evidence="15">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="347"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="CC/SHH/C" evidence="12">
        <location>
            <begin position="15"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="255"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="779" mass="88673" checksum="160D231C97168AEA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ92</accession>
    <name>I7AQ92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263979.1">
        <property type="nucleotide sequence ID" value="XM_009265704.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ92"/>
    <dbReference type="GeneID" id="20520765"/>
    <dbReference type="KEGG" id="ero:EROM_020070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020070"/>
    <dbReference type="HOGENOM" id="CLU_630089_0_0_1"/>
    <dbReference type="OrthoDB" id="2192376at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="436" mass="50482" checksum="F32E58B08768FB3F" modified="2012-10-03" version="1">MKILLLSPFVFAHVINLVILYEKTGLKEVKEMYHYVVERLLDKFNEHLQRRGIRISLLDFLAYDDYLVKPGYQDISIMGGEESLDARVEALSGIPANVILVASASPEEEREKFIPNSPCMSRFISNMVSSDIVDDELLTKIIYAVLDWMGGLFKIEVPSPVKERNPEIFDKFAKDVTTPEFIESLKQCTKETRDKFEKFIRVLNWNEEKMFSLRMMSGRLDSGQSDGVEISSPLKWVLSKSEKKAEDTNSQSQPPKEIKQEKDNPESSSNEESSSEKKEKNTNKKPSRSHEPEISSKKIKKTSLKKTNSDNLVDLEESEKKEEIEEHEKRKTNNSLEYLDKINSLRNDQKPREEEALMEEKIREELQKIISHESHNLKRRFDGYPALTRIPSYNDEEVRDAQPRILFPSRATPVRNASKINRGLSYGKIPYRRGYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQB6</accession>
    <name>I7AQB6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rho Ras-like GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264004.1">
        <property type="nucleotide sequence ID" value="XM_009265729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQB6"/>
    <dbReference type="GeneID" id="20520790"/>
    <dbReference type="KEGG" id="ero:EROM_020320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020320"/>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="212" mass="23644" checksum="A74425EB75817A26" modified="2012-10-03" version="1">MVEIATADISKQLTIIGSATCGKTSILTRHVKRTFGTEISPTVFNSVSNTYKHKDYVVELRLWDTAGQDEYARFRHLALPSADYIMIVYAIDDRSSYAEVKNSLVGQVKEKAPQAKYFLVGAKIDLREDCKRLEELARSGDSPITTSEGLELSQSIGAIGYFECSAKNNIGIDDIFEKISKYALKAHLKESRSFIGGILASLWDFLCCRKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQN1</accession>
    <name>I7AQN1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111880</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265666.1">
        <property type="nucleotide sequence ID" value="XM_009267391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQN1"/>
    <dbReference type="GeneID" id="20564787"/>
    <dbReference type="KEGG" id="ero:EROM_111880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111880"/>
    <dbReference type="HOGENOM" id="CLU_030988_13_3_1"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="9"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="19250" checksum="2A60EC30EED4261C" modified="2012-10-03" version="1">MAELSSDSAAIKRIKKEHELLENDRGNEKRKDSTSKYIKIVPFVSGDFFTWEAYLTAPEESVYKGGVFKLLITFSNEYPFKPPKITFKTPIYHPNINSSGMICIDILGKEWSPALTLNSVLLSLLTLLDDPNADDPLRPEVADIYKTNKEEYRQRCRESARKAMENKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR44</accession>
    <name>I7AR44_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264314.1">
        <property type="nucleotide sequence ID" value="XM_009266039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR44"/>
    <dbReference type="GeneID" id="20521113"/>
    <dbReference type="KEGG" id="ero:EROM_040490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040490"/>
    <dbReference type="HOGENOM" id="CLU_829072_0_0_1"/>
    <dbReference type="OrthoDB" id="2191682at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="337" mass="39993" checksum="9084C5C3555DC845" modified="2012-10-03" version="1">MKPSRCLKKLLKIDISPDSSYSEIEIGLYNLKRIVKSTSQKEVVRGGVRKMALLFHSKNWIARSLALKYFSKINKYVDEVDTNILHMFYSTDPRINDLAVKYVDIFSRFFKDNDTVFYHVYRSRSKYRRGAMKSLISQSPRYRPYEKDCEGEDDDLDLFKNIRRNTAEEYFGRLALPKLVRLSSTYPCLIKYFKFTEKFLMEEVRKADRYPPDMLRRLEMILGIEKEGRTMWDEFMKCSRWMEHGKFDRSVRVLKRLQNLDISKEYKIIFGIFARKMMFCLGLGNEMSVESLRPVISGHFEAELSGISQNGVYRVLRRLFKNGMKRMDTGDRKTKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARB9</accession>
    <name>I7ARB9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264409.1">
        <property type="nucleotide sequence ID" value="XM_009266134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARB9"/>
    <dbReference type="GeneID" id="20521211"/>
    <dbReference type="KEGG" id="ero:EROM_041480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041480"/>
    <dbReference type="HOGENOM" id="CLU_156029_0_0_1"/>
    <dbReference type="OrthoDB" id="2189461at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="14381" checksum="45755A25C514381F" modified="2012-10-03" version="1">MVKFKLVKTVTYVGISPEFLGNPGRAVSKYISPKLLIYSHKINGVPLSFDIKKIGCAEASVCETGDVITPVSVEYVVLMVPIGEMLNSIEGRAFGIFDTDVDGNKEYTGNFIVKEIRLKSRSFFTIVGTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARJ5</accession>
    <name>I7ARJ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264494.1">
        <property type="nucleotide sequence ID" value="XM_009266219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARJ5"/>
    <dbReference type="GeneID" id="20521297"/>
    <dbReference type="KEGG" id="ero:EROM_050650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050650"/>
    <dbReference type="HOGENOM" id="CLU_2687826_0_0_1"/>
    <dbReference type="OrthoDB" id="2190674at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="74" mass="8656" checksum="99D4C011A269E7C6" modified="2012-10-03" version="1">MEKMEKEYDIIQIPKGIKRSDLPERIEADKPFEIDGSLYVCEKRDEGAMKVCFEEEGKTSIPVLSKNFYVVRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARK0</accession>
    <name>I7ARK0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GPI-anchored wall transfer protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_050700</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264499.1">
        <property type="nucleotide sequence ID" value="XM_009266224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARK0"/>
    <dbReference type="GeneID" id="20521302"/>
    <dbReference type="KEGG" id="ero:EROM_050700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050700"/>
    <dbReference type="HOGENOM" id="CLU_020802_3_0_1"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="201"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="331"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="399" mass="45850" checksum="E11D390ED9555078" modified="2012-10-03" version="1">MKNTNELELLQITGMSALSLMVYSSGYRKTMLNDFLFCIVPQYCAIMFPNEIWILYLCLVILAKYPKIRRSKETTNEKTSVIDHLRFTISALVVIAIYGVDFTMFDKRLGKSHFLGISLMDIGVGSFIYNSGVVRCKSRFKRKHVKSYLILILLGFVRFFVVKWFNISVNPKEYGTHLNFYFLLAIVNFICSMVKSRYNFLLGLLVITVYEGVLRFSRLMFFIFSDERRTFLEKNKEGLTAIISYVSIFLMATEVGRICLSQGKIRRKALRVLGMSGIFACLYVTSCFSCEGSRRLGNAAFVFWILALHTFHVGIYMLLESTIVMYNPLVVMFCSSNMMFIFLFSNLIVLVGNTFFDLKSFSPIAGHLNMLVYLISVFLAPALLASRFDLRWIGLGYRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ART9</accession>
    <name>I7ART9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">transketolase</fullName>
            <ecNumber evidence="8">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_060020</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="8"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264594.1">
        <property type="nucleotide sequence ID" value="XM_009266319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ART9"/>
    <dbReference type="GeneID" id="20521399"/>
    <dbReference type="KEGG" id="ero:EROM_060020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060020"/>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_1"/>
    <dbReference type="OrthoDB" id="10267175at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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        <location>
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        </location>
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        </location>
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        </source>
    </evidence>
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        <source>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022842"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049473"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83097.1"/>
        </source>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="624" mass="69981" checksum="AAC57701F96DFDB8" modified="2012-10-03" version="1"> </sequence>
</entry>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
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</entry>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
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    </dbReference>
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        <location>
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            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="582" mass="67695" checksum="E20FE00E0A6E75D8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS75</accession>
    <name>I7AS75_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">EROM_070160</name>
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    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83267.1"/>
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    <dbReference type="OrthoDB" id="2191581at2759"/>
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    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="888" mass="102774" checksum="FEBA3E84FFD9F3DD" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASJ1</accession>
    <name>I7ASJ1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_071510</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
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            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
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        <location>
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            <end position="946"/>
        </location>
    </feature>
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            <end position="348"/>
        </location>
    </feature>
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        <location>
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            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="851"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="756"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="866"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="946" mass="105860" checksum="DDB6C3CE092700E7" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASP4</accession>
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            <fullName evidence="4">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
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    </protein>
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    </gene>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
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    </dbReference>
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    <dbReference type="OrthoDB" id="2187509at2759"/>
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        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="210" mass="24288" checksum="D1F46E4D4340CF27" modified="2012-10-03" version="1">MEKFSKYNDPFSGINPFVNSRRSSISIFGYFKILLKIPLVLLLLGTNINVVQFLIRINSNKKVKPKVLASNASSFLDIFVLKYLTGINNFYYVTESGFMDARNGRFYKKIAEPCVLFPEGCQTNNRAILQFVRNVEVDHVCGIRYKGECINMYGNFMRFIFGFLASRNIVDVRFKKSSDLDDICKLSSLPQVKWTSKDKDRFMEEFVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="7">Sulfate permease-like protein</fullName>
        </submittedName>
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        <name evidence="7" type="ordered locus">EROM_080450</name>
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    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Microsporidia</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    <dbReference type="GeneID" id="20521781"/>
    <dbReference type="KEGG" id="ero:EROM_080450"/>
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    <dbReference type="OrthoDB" id="2190497at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052706">
        <property type="entry name" value="Membrane-Transporter-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310:SF4">
        <property type="entry name" value="AFR304WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43310">
        <property type="entry name" value="SULFATE TRANSPORTER YBAR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="82"/>
        </location>
    </feature>
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        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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            <end position="188"/>
        </location>
    </feature>
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        <location>
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            <end position="217"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="323"/>
        </location>
    </feature>
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        <location>
            <begin position="357"/>
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        </location>
    </feature>
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        <location>
            <begin position="390"/>
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        </location>
    </feature>
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        <location>
            <begin position="50"/>
            <end position="392"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="543" mass="59762" checksum="4A76D2D56B3A34B1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT17</accession>
    <name>I7AT17_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Diphthine--ammonia ligase</fullName>
            <ecNumber evidence="1">6.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Diphthamide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diphthamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081810</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>diphthine-[translation elongation factor 2] + NH4(+) + ATP = diphthamide-[translation elongation factor 2] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10174"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16692"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.14"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.3.1.14" evidence="1"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT17"/>
    <dbReference type="GeneID" id="20521916"/>
    <dbReference type="KEGG" id="ero:EROM_081810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081810"/>
    <dbReference type="HOGENOM" id="CLU_010289_0_0_1"/>
    <dbReference type="OrthoDB" id="686384at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000145">
        <property type="entry name" value="ATP-binding domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00029814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="248" mass="28278" checksum="CC59C67E0A556325" modified="2012-10-03" version="1">MRFLALASGGKDSLYAIHCLQQEGHRAVGLLHMRCSDGYQDSFMYQTVGSEVADVLGECLNIPVFIYQTKCRSINQSLEYNREEGDEVEDLYTAISRAKEKVYFEGVSSGAILSRYQKSRVENVCSRLFLKCLSPLWEMDQKRLLLDMISNGMDGRIVKIASSILGKECINMRIDEIYEYLEAAKEEVNFCGEGGEYETIVLDCPMFKKRINIDEYEVISHPEEIGKEGCVFYMKIVKMSLAEKRSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATC1</accession>
    <name>I7ATC1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265169.1">
        <property type="nucleotide sequence ID" value="XM_009266894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATC1"/>
    <dbReference type="GeneID" id="20564279"/>
    <dbReference type="KEGG" id="ero:EROM_090550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090550"/>
    <dbReference type="HOGENOM" id="CLU_000134_46_3_1"/>
    <dbReference type="OrthoDB" id="2423701at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072380">
        <property type="term" value="C:TRC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047150">
        <property type="entry name" value="SGT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45831:SF2">
        <property type="entry name" value="LD24721P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="44"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="112"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="233" mass="26434" checksum="28A5BF96AFF8AA48" modified="2012-10-03" version="1">MNLKEIERYFNKKRQYLSKEEYKTIVGALKKLDLAEKASNKQLAEDMKSKGNEEYNDGDFRSAIDSYTQAIIYDPTNVVYLSNRAAAYSKLGMVENAIEDCENGLNIDDKFVKLYIRLGMLYLSKDKNRAYQIFERGLEVDPKNKVLKKQLDLLSKGASDTSLENTQAASLDDMIKNMGMGGLKDSKIDFNSLFNNKNIRDVLDTVVKDKSPSDLMNMVKDMIGAMGSQDNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUK5</accession>
    <name>I7AUK5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265639.1">
        <property type="nucleotide sequence ID" value="XM_009267364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUK5"/>
    <dbReference type="GeneID" id="20564760"/>
    <dbReference type="KEGG" id="ero:EROM_111610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111610"/>
    <dbReference type="HOGENOM" id="CLU_055474_1_0_1"/>
    <dbReference type="OrthoDB" id="2195113at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042175">
        <property type="term" value="C:nuclear outer membrane-endoplasmic reticulum membrane network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008298">
        <property type="term" value="P:intracellular mRNA localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039604">
        <property type="entry name" value="Bfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027:SF2">
        <property type="entry name" value="LEBERCILIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31027">
        <property type="entry name" value="NUCLEAR SEGREGATION PROTEIN BFR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="20"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="388" mass="44680" checksum="91FFBF24950E0A88" modified="2012-10-03" version="1">MQQENRRKRIPGIHSYLAVIGELRQELGKVTASINDLRDKISSIYKQERENSPKNDLYKKLDELGSEIKSLKEDRSKAFNSKSEALGAYETIKGEVQPEKGKKMMSAQEIDNRMREINLKLISTKCDSKMEKMFESEIENLRKQKKNIGVLEQKSKLALDMKAKLDVLNSEIKDLSQRIAEKQSIVDGIKAELKEINDQEKPKNPTIEGYEKNIQALKSKRGELSEKIKAQQEKIREKEIEYDKFLEEMAIAQALEKQKEEIKQRISALEEQKNALSKEESKLNPSKFDSIIFRMGSLDLSGEKVSLPVDLALYLSQHKIPIPTSPGQMNPTIEVLKSQKKDFASQVVEKRKEFESRISDLERKISEERKVLTAMPPTDVRVLKFKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGW9</accession>
    <name>I6ZGW9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010890</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263930.1">
        <property type="nucleotide sequence ID" value="XM_009265655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGW9"/>
    <dbReference type="GeneID" id="20520716"/>
    <dbReference type="KEGG" id="ero:EROM_010890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010890"/>
    <dbReference type="HOGENOM" id="CLU_033000_0_0_1"/>
    <dbReference type="OrthoDB" id="2193595at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="353"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="422"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="495"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="589" mass="68400" checksum="277DE2DE2606951B" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGX8</accession>
    <name>I6ZGX8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010990</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263940.1">
        <property type="nucleotide sequence ID" value="XM_009265665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGX8"/>
    <dbReference type="GeneID" id="20520726"/>
    <dbReference type="KEGG" id="ero:EROM_010990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010990"/>
    <dbReference type="HOGENOM" id="CLU_023644_0_0_1"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016688">
        <property type="entry name" value="MscS-like_plants/fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618:SF1">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31618">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="404"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="5">
        <location>
            <begin position="451"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="627" mass="72354" checksum="6381D9DC62172F09" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGZ2</accession>
    <name>I6ZGZ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Frataxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_011140</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the frataxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263955.1">
        <property type="nucleotide sequence ID" value="XM_009265680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGZ2"/>
    <dbReference type="GeneID" id="20520741"/>
    <dbReference type="KEGG" id="ero:EROM_011140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011140"/>
    <dbReference type="HOGENOM" id="CLU_080880_4_2_1"/>
    <dbReference type="OrthoDB" id="1897642at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034986">
        <property type="term" value="F:iron chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.10">
        <property type="entry name" value="Frataxin/CyaY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002908">
        <property type="entry name" value="Frataxin/CyaY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036524">
        <property type="entry name" value="Frataxin/CyaY_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821">
        <property type="entry name" value="FRATAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16821:SF2">
        <property type="entry name" value="FRATAXIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01491">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01219">
        <property type="entry name" value="Frataxin_Cyay"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55387">
        <property type="entry name" value="Frataxin/Nqo15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50810">
        <property type="entry name" value="FRATAXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0410">Iron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="124" mass="14474" checksum="CC92ABE031127F4B" modified="2012-10-03" version="1">MLHRQALREGITKPLYHRLADEALANLSEHLENELYDGYADYKGDNLQVKVENVGEYMFNKQPASMQIWTSSPITGPRKFDIYRSYEWIDTKNNISLSKYVEEELKKIQSKLNRKANNMGSCLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH29</accession>
    <name>I6ZH29_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263995.1">
        <property type="nucleotide sequence ID" value="XM_009265720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH29"/>
    <dbReference type="GeneID" id="20520781"/>
    <dbReference type="KEGG" id="ero:EROM_020230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020230"/>
    <dbReference type="HOGENOM" id="CLU_506320_0_0_1"/>
    <dbReference type="OrthoDB" id="2191709at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="500" mass="58369" checksum="6A0B59E85BA7C30D" modified="2012-10-03" version="1">MVEDEARGALFILNESLVTGSRLSPGYKESLEKIVSLTSEDLHLLRFLGGWGFIGKQFPYLIRDSEVRHEAFRLATRILQDPASNLPVLFMTRKGGFLLSEALYESMDNPQDIIRFCEEAAKKNSLLPGVLYDCGFFILLNSLVCEETARFYSSIFAYRMDEAKREYKGVGGGDVMHEIKRRYFDPEIVFKDRAHLSFKEPPSLHKVGHQVLKNIFDDVYNTTFFNMLELGLDKSLDLMNYCEWDYLFLCGSNELRDLVSSGSYLAIRLYRKMLESLRKCTRAVFYLGKVEDGDLMMHIAKFLESRDVHVVMECALILYNYYYLFNWNLDRKQFNENRIRRIFCLLEYSYSDCSCLQKCIYSQPDFLPGISEEEEQRTGCLNDCNTMKILRLLSKIYALVDKRYFYKPSFIVLMKTWNDVFERHNCWNNTFFADFFTDITSFLRANPYNVARIVFPFKKPRKKTKNVIDEKNDDGDSSTCVSILASEIDLSEVESLDELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHM1</accession>
    <name>I6ZHM1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease</fullName>
            <ecNumber evidence="9">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_030570</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase HII family. Eukaryotic subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="9"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264175.1">
        <property type="nucleotide sequence ID" value="XM_009265900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHM1"/>
    <dbReference type="GeneID" id="20520968"/>
    <dbReference type="KEGG" id="ero:EROM_030570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030570"/>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_1"/>
    <dbReference type="OrthoDB" id="7462577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07181">
        <property type="entry name" value="RNase_HII_eukaryota_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
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        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
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        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF7">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="10">
        <location>
            <begin position="17"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="26783" checksum="9F2DFCD249726FA3" modified="2012-10-03" version="1">MENKNVFFSSLKKISEEVVVGIDEAGRGPVVGYMVYGALVAPKSALKSTGFKDSKVLTHAQRHTFFQTIKEKGLGYVYHCTHPDYITEMMQLGSTSLDEISVNSVFRILDEIKAKCENVEGVYVDALGNCEKYKARLKKKYPYMFVVEKRADSRFQIVSGASIVAKVQRDLMISEFGNDLGSGYPSDPSTIRWLKRNTNAVFGFPPGVRYSWSTVKKILGERRSKPLKGALSGFYFNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHM6</accession>
    <name>I6ZHM6_ENCRO</name>
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        <submittedName>
            <fullName evidence="7">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_030670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009265910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHM6"/>
    <dbReference type="GeneID" id="20520978"/>
    <dbReference type="KEGG" id="ero:EROM_030670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030670"/>
    <dbReference type="HOGENOM" id="CLU_002678_2_1_1"/>
    <dbReference type="OrthoDB" id="2188412at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF295">
        <property type="entry name" value="AZ2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="17"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="185" mass="22179" checksum="D0376EF42235D2E2" modified="2012-10-03" version="1">MTIIKFFCSNCPWSMTFQCHFEGCEKSFPRRSKLNDHLNTHTKNRPYKCDMCEKSYMKNSHLSVHKKTHFPPEFECPRCGRMCHTRDKLSKHQKTCLEYKCDTCGKKYKKKSWFDAHVESHHVKIFNVRKLRHVCEYCKFEFSRKGNLLTHIRSVHFLVKPFKCLCGKEYAHNTSLHSHKKRCVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHP1</accession>
    <name>I6ZHP1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SCP-like extracellular protein domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264205.1">
        <property type="nucleotide sequence ID" value="XM_009265930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHP1"/>
    <dbReference type="GeneID" id="20520998"/>
    <dbReference type="KEGG" id="ero:EROM_030870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030870"/>
    <dbReference type="HOGENOM" id="CLU_478979_0_0_1"/>
    <dbReference type="OrthoDB" id="568194at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="CDD" id="cd05379">
        <property type="entry name" value="CAP_bacterial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31157:SF1">
        <property type="entry name" value="SCP DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707300" evidence="2">
        <location>
            <begin position="18"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="SCP" evidence="3">
        <location>
            <begin position="20"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="429"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="481"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="569" mass="62947" checksum="64D972E01A8470BE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHP5</accession>
    <name>I6ZHP5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription initiation factor TFIIIB subunit Bdp1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264210.1">
        <property type="nucleotide sequence ID" value="XM_009265935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHP5"/>
    <dbReference type="GeneID" id="20521003"/>
    <dbReference type="KEGG" id="ero:EROM_030920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030920"/>
    <dbReference type="HOGENOM" id="CLU_134553_0_0_1"/>
    <dbReference type="OrthoDB" id="272624at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001156">
        <property type="term" value="F:TFIIIC-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070898">
        <property type="term" value="P:RNA polymerase III preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039467">
        <property type="entry name" value="TFIIIB_B''_Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929">
        <property type="entry name" value="RNA POLYMERASE III TRANSCRIPTION INITIATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22929:SF0">
        <property type="entry name" value="TRANSCRIPTION FACTOR TFIIIB COMPONENT B'' HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15963">
        <property type="entry name" value="Myb_DNA-bind_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb-like" evidence="2">
        <location>
            <begin position="83"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="160" mass="18932" checksum="415F4866F5F77BEF" modified="2012-10-03" version="1">MDQKLKHFLDNSNFRKDRRGKKKKVEVSGGKSEERQEEILMIKDGEIVVNDANMYVDTHKDMEMEVMEDDRIVTSSTFSKKKGALRWSKKEIELFYKALEICGIEFSLISSLFPNKERKHIKAKYIKEAKVNPERINEVLNEYKTFDQVAYNKLRSYLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI29</accession>
    <name>I6ZI29_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264370.1">
        <property type="nucleotide sequence ID" value="XM_009266095.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI29"/>
    <dbReference type="GeneID" id="20521170"/>
    <dbReference type="KEGG" id="ero:EROM_041060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041060"/>
    <dbReference type="HOGENOM" id="CLU_2121042_0_0_1"/>
    <dbReference type="OrthoDB" id="2190934at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="113" mass="12909" checksum="8C280513DC4AF66F" modified="2012-10-03" version="1">MPEATILSEALPQVRPQQGSETKESSSKEVLPKDMPVVNVKDIMMYAENMEKLESKKLIPYVVYLDEQFKEIVQKRRKDAKVVLILYIVVMSILVIGLFVCGIKLFGYLMGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI39</accession>
    <name>I6ZI39_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hpc2-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264385.1">
        <property type="nucleotide sequence ID" value="XM_009266110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI39"/>
    <dbReference type="GeneID" id="20521185"/>
    <dbReference type="KEGG" id="ero:EROM_041220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041220"/>
    <dbReference type="HOGENOM" id="CLU_842059_0_0_1"/>
    <dbReference type="OrthoDB" id="2196297at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014840">
        <property type="entry name" value="HRD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08729">
        <property type="entry name" value="HUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hpc2-related" evidence="1">
        <location>
            <begin position="23"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="328" mass="38291" checksum="7C126456075227E8" modified="2012-10-03" version="1">MNPENEVTIQVNVMEPKEIDLRKKEKQKKRRQRTDEYDYNDPFIEPFEGETQMVMIESSLEDFFVYKGELPYSAKKVLSVHKARERAKLMKSSSIDVSGQKAEKRGKDPKRLRISVGKGRRRRIPKSDTKICDCLASLIKSEVGNTGEVPRDESDYMEYYVSLKVLEAFQKEREVDWRIQNLDALKKPSDEEIARYLKKSESAMNELFNTIAGEIQNYQLYSEDLSLFKGFQKESFLFNTCKYFLLFIKISFMGQEGMTFNKARKEAYNNVLGLFPNTCKNSTQTQYYLAKRISQTCEMKDFAECNNKEHKKGNEDRNVSSNLESIVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI73</accession>
    <name>I6ZI73_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">GTP-binding nuclear protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_041590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ran family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264420.1">
        <property type="nucleotide sequence ID" value="XM_009266145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI73"/>
    <dbReference type="GeneID" id="20521222"/>
    <dbReference type="KEGG" id="ero:EROM_041590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041590"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_6_1"/>
    <dbReference type="OrthoDB" id="48625at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00877">
        <property type="entry name" value="Ran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001447">
        <property type="entry name" value="Ras-related protein Rab-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002041">
        <property type="entry name" value="Ran_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071:SF0">
        <property type="entry name" value="GTP-BINDING NUCLEAR PROTEIN RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24071">
        <property type="entry name" value="RAN GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51418">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="214" mass="24362" checksum="315657906C06D437" modified="2012-10-03" version="1">MERRELTYKICLIGDGGVGKTTYINRVLDGRFEKNYNATVGAVNHPVTFLDDQGNVIKFNVWDTAGQEKKAVLKDVYYIGASGAIFFFDVTSRITCQNLARWVKEFQAVVGNEAPIVICANKIDIKNRQKISKKLVMEVLKGKNYEYFEISAKTAHNFGLPFLHLARIFTGRPDLIFVSNVNLEPTDVNYDYHSPEETKYIDYMEQAAKMAPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI85</accession>
    <name>I6ZI85_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0328 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264430.1">
        <property type="nucleotide sequence ID" value="XM_009266155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI85"/>
    <dbReference type="GeneID" id="20521232"/>
    <dbReference type="KEGG" id="ero:EROM_041680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041680"/>
    <dbReference type="HOGENOM" id="CLU_1740511_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019081">
        <property type="entry name" value="UPF0328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09591">
        <property type="entry name" value="DUF2463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="150" mass="16856" checksum="FFD9328A294BF504" modified="2012-10-03" version="1">MAIYEWANEDPNIFSMVIPSILVLPPYLLSTSCDLKPCKFFFTDTGIDILLSLLVLLHIPLGVGLLCGGTKYQLYFAITSFFATLAKSLKEVCLPSRKYEDSTRKWRLAILIFILLSAAIICGITIFVFLETFKNYSTSIKKTSGNLRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIL6</accession>
    <name>I6ZIL6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="7">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_051330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="7">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="7"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264560.1">
        <property type="nucleotide sequence ID" value="XM_009266285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIL6"/>
    <dbReference type="GeneID" id="20521364"/>
    <dbReference type="KEGG" id="ero:EROM_051330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051330"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_1"/>
    <dbReference type="OrthoDB" id="10261904at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="86"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="284"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="425" mass="48551" checksum="6D298DEABD9D2F2B" modified="2012-10-03" version="1">MIKVTIYFIFYNTLMRNLAILRSLRDKILKKTSGRRPMARVDIVQDTPQSSETTTSNTKGSQWKWPFLLNRDLMPPYPTDVQRIVIPHILAKESIGVVSKTGSGKTLSYVLPYIHILESEDKRLLVIVPTRELVSQVGQIFRKFSEDSLKVVEITGEMGIDSQRLQVSEPFDVIVSTPGRLKELVKLKSIGGFEYVVIDEADRLMQHDFRNDVTFILEEAGAKVISLFSATPFEWNGVTKISVGDVSVSRSVEEFFLYAEENEKQGILEDIFESCEDWLMKNMNVKTKCLEIKKIVVFCNTIKTCESLHKRFKGSLVLHSRRTMEERKAVVEALEEEKVVLIATDLGGRGIDIRDVDLVINYDLPKTIEVYVHRCGRAGRQKEGMSLSMVCREDKELLPKLKRLIERKGGVVPEFMDIKEDIILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ44</accession>
    <name>I6ZJ44_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methionine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_061470</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264735.1">
        <property type="nucleotide sequence ID" value="XM_009266460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ44"/>
    <dbReference type="GeneID" id="20521544"/>
    <dbReference type="KEGG" id="ero:EROM_061470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061470"/>
    <dbReference type="HOGENOM" id="CLU_007946_3_0_1"/>
    <dbReference type="OrthoDB" id="2190245at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050598">
        <property type="entry name" value="AminoAcid_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11785:SF512">
        <property type="entry name" value="SOBREMESA, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="394" mass="43080" checksum="7AADB66B73ABD7B1" modified="2012-10-03" version="1">MEKKIEMLDLLFLSICNMVGIGVFSLAHYVLSQTGTCASSIFLIVASAIIATVFGLCYAELGSTYPYAGGDLNYLSRAYTKHLGTIYSVVSILLILPLSCAIMSIKIHECFEEDIGRDYCIAMLLGLCTIFLSFGGKLVTWTMRILFFLKVVTVASLLVISSISFIVARNAPNPAILDKRNSTVEIDATSIVKGLCFTQFSFDGWNCGNYIANRIHNPGKSFPKAIVGSIWVVTFIYILISLSMMCVVPYDDIVGNVFFIDEYFRAVGIPITPRMLSVMVTLIPTFGSLVCSLIVGTGIIESLVPAKFSKKLEVISLAAFSFIVFVFAKLESINWMVSKIAFGISLFYSLSCMGLVVLKLKHPSIERPFNLPLAVPLAASLIGISICSYIICCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJM1</accession>
    <name>I6ZJM1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA replication licensing factor MCM3</fullName>
            <ecNumber evidence="11">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_080210</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="11"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264940.1">
        <property type="nucleotide sequence ID" value="XM_009266665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJM1"/>
    <dbReference type="GeneID" id="20521757"/>
    <dbReference type="KEGG" id="ero:EROM_080210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080210"/>
    <dbReference type="HOGENOM" id="CLU_000995_6_0_1"/>
    <dbReference type="OrthoDB" id="1882346at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002469">
        <property type="entry name" value="Cell division control protein 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008046">
        <property type="entry name" value="Mcm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF46">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01659">
        <property type="entry name" value="MCMPROTEIN3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="12">
        <location>
            <begin position="269"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="688" mass="77403" checksum="CC5725EDFD8A51F0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJR5</accession>
    <name>I6ZJR5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rab5-like GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264985.1">
        <property type="nucleotide sequence ID" value="XM_009266710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJR5"/>
    <dbReference type="GeneID" id="20521805"/>
    <dbReference type="KEGG" id="ero:EROM_080690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080690"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="OrthoDB" id="63533at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01860">
        <property type="entry name" value="Rab5_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000823">
        <property type="entry name" value="Small GTPase RAB, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47978">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="197" mass="22693" checksum="8DC352993476A3B7" modified="2012-10-03" version="1">MPAFKENMKTYTFKVVVLGYYSVGKSSLVLKYVKGEFNPNEESTIGASFLTKTIFTQEGSIKFEIWDTAGQERYNSLIPMYYRGAQVALIAYDITSMESFETAKRWVEELSFEKPKEFIKVLIGNKSDLESDRKVEYQKGKEYADSQNLLFFESSAKTGKNIAEIFNAIAERVPKEEAKKKTKGFRLKDDYIKYLCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJZ9</accession>
    <name>I6ZJZ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081720</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265085.1">
        <property type="nucleotide sequence ID" value="XM_009266810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJZ9"/>
    <dbReference type="GeneID" id="20521907"/>
    <dbReference type="KEGG" id="ero:EROM_081720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081720"/>
    <dbReference type="HOGENOM" id="CLU_1540031_0_0_1"/>
    <dbReference type="OrthoDB" id="2192071at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="174" mass="20534" checksum="4A0A2DE5E9DD1A22" modified="2012-10-03" version="1">MEKMADFPFSMPLRFGKGPGMSFSLSRREDIGNHNRKVFEAITALRKDRNVLTYVKNPEFFEDSSEEEGYSGDNHMIREIVTCKYYEDGRYKFFGNLVLRGFSRNRVRYVNMRKGKRVIFKDLEKSQVKLVYNKGEDVVYMLMLKSMIIYKVVCRHPTIASNIYNHLMDLDPNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK27</accession>
    <name>I6ZK27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_082040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265115.1">
        <property type="nucleotide sequence ID" value="XM_009266840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK27"/>
    <dbReference type="GeneID" id="20521939"/>
    <dbReference type="KEGG" id="ero:EROM_082040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_082040"/>
    <dbReference type="HOGENOM" id="CLU_1057795_0_0_1"/>
    <dbReference type="OrthoDB" id="2195135at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="191"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="263" mass="30297" checksum="F14E3B2CFFE4E3F7" modified="2012-10-03" version="1">MNMINEAVDGKKNERQKHKEPTDSLPKSKSWKDFPDTWLSLILTLMTGIMVFFRSSFRTKVENIFSQIIGWFNLGRLIYFTAKRLYFRIEGSKKLKGNDVAMIVCLLLHLLMLAFLVCSNFIFPGIGIHKMATALERNPIGIFFASIFFLNAQLVMEPHNTYSVLVVGLEAILTAWDVEGFDYEKDVIKNLWTRLFISIFVGVMLWIVEDWIVDSLNISKRNVRKIRNFMKIVVALIYTIGGYCIMNMIFLNASNTNSQPVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKC5</accession>
    <name>I6ZKC5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">histone deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone deacetylase family. HD type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="2"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265220.1">
        <property type="nucleotide sequence ID" value="XM_009266945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKC5"/>
    <dbReference type="GeneID" id="20564331"/>
    <dbReference type="KEGG" id="ero:EROM_091070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091070"/>
    <dbReference type="HOGENOM" id="CLU_007727_7_4_1"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09991">
        <property type="entry name" value="HDAC_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="8">
        <location>
            <begin position="20"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="343" mass="38394" checksum="2A18F253127AB3BA" modified="2012-10-03" version="1">MAIAYVFDEEVGLFHYGPRHPMKPFRTVVTHSLVKSFGLDRKMTIVKPATASLTYHTKEYLENLGKIETPDCPDIIGLSRFCELYGSASINSAMILSEGKYNTVINWSGGLHHAHKNVPSGFCHVNDIVMAILELLKTYRRVMYIDIDVHHGDGVEEAFLESDRVLTLSLHKYGDGFFPETGTLITTGHKAVNVPLLSGIDDPSYKYIFEPIVESCVKKFSPDAIVLQCGADSLGEDRLGCFNLSIEGHASCVRFVNSLGIPLLCLGGGGYTLTNVARCWAYETAVLCGEDPIIDIPRENPFYTYFSPTYTINPVFKRKYANKNDREYLDMIMSFILDKVDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKH4</accession>
    <name>I6ZKH4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD40 domain-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_091770</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat ESC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265290.1">
        <property type="nucleotide sequence ID" value="XM_009267015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKH4"/>
    <dbReference type="GeneID" id="20564401"/>
    <dbReference type="KEGG" id="ero:EROM_091770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091770"/>
    <dbReference type="HOGENOM" id="CLU_032683_0_0_1"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051243">
        <property type="entry name" value="PcG_WD-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10253">
        <property type="entry name" value="POLYCOMB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="137"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="311" mass="35155" checksum="7F23123337A47EBF" modified="2012-10-03" version="1">MSSIIEIPNACFESMSICNKHRIVSLAGKRSSVIVSYRDGLKVVQRILDEHPDENFQCSEFFMAGNDISLALGGKLGIIKMVNLSKGTFIGHIKAHGGSISSIKRYKDKYLLSCSEDTTIKMWDISELACVCIFGGYSGHRDYVLSIDVSNDMRYLASCGTDCSIKIWRIPSYLNKLECTTPIYSSTHECRFPIECIRFYGELLVFYSGEKRIHVISLKYEESKSSAEALLAGEIILEERLLRKFDISGSTLVALIESQDVIMFDMNDIGFRSHPKTLKSFKRKKVQDFCAVGDQIFILLEDSRFMTMDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKI3</accession>
    <name>I6ZKI3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PHD zinc finger-containing putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091890</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265300.1">
        <property type="nucleotide sequence ID" value="XM_009267025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKI3"/>
    <dbReference type="GeneID" id="20564413"/>
    <dbReference type="KEGG" id="ero:EROM_091890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091890"/>
    <dbReference type="HOGENOM" id="CLU_056968_0_0_1"/>
    <dbReference type="OrthoDB" id="20839at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15571">
        <property type="entry name" value="ePHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15492">
        <property type="entry name" value="PHD_BRPF_JADE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13831">
        <property type="entry name" value="PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13832">
        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51805">
        <property type="entry name" value="EPHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="4">
        <location>
            <begin position="187"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="404" mass="46986" checksum="A4373BC52AE166FF" modified="2012-10-03" version="1">MLTCRGKKFSIPYGESYHPKMSTSQSFGSIPREERSYKSIYDGIDLLRMHHIVADGIHPPEEKLKKKQTIQEEQGKDFFESILYKVDSYDRHYLHGSHPNVSENVFELIMDRLEKEWFFFVQGLVTRYVKPLRPSSFCDICTKYTSENDKALVVCQGCEICVHENCYGIQDLSDFWLCRRCIYREDSKECSFCISTNGVFKQTSDNKWGHVLCVMFNRSLSFGHPLSKEPIDVGSYTKESGCLFCDEVRGTVICCSYFACSKKYHVPCALDKCYFDLSNRLSYCIVHSPLKENTCELGHRKISAMKRFGYEKLSHPPIIRKKVPMACPKPTLFSKLSNLQPIATPSIIWRIKTYDLKGKYGKDMLKILKYWELKRRTVGEPLVVHPDILPGRWAEESWINKRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKN2</accession>
    <name>I6ZKN2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265365.1">
        <property type="nucleotide sequence ID" value="XM_009267090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKN2"/>
    <dbReference type="GeneID" id="20564482"/>
    <dbReference type="KEGG" id="ero:EROM_100520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100520"/>
    <dbReference type="HOGENOM" id="CLU_946746_0_0_1"/>
    <dbReference type="OrthoDB" id="2190976at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="294" mass="33840" checksum="F7CF4B899C58E2E2" modified="2012-10-03" version="1">MDVQGLREEREACIRERVLLADEMDSFIVGHIGEIREVLTIGEAISSMICSEELLMFVSWRVSRLVELIRDKKIEVLSDLLEKRIDCKCLEDILAAEYCCKKVLSNDTIECPVLDIDVCIDVVNGIVDIKGDRFRRIIVGTDKVLHRSLDKLFSEAFHMKVSKELMLSYIFNVKLEMGLEIFRSLYEKPLEVLKSLASYKYYKEKGIHILIKKDEVPHHLLESVRAEVEKEIAGIIGMESSDEKKVLLINYLLVMFGGIGSDLDLSYFDGKKEGYVLDWRKFENNTFTQNTNIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKP0</accession>
    <name>I6ZKP0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methionine aminopeptidase 2</fullName>
            <shortName evidence="6">MAP 2</shortName>
            <shortName evidence="6">MetAP 2</shortName>
            <ecNumber evidence="6">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_100620</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="6"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265375.1">
        <property type="nucleotide sequence ID" value="XM_009267100.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKP0"/>
    <dbReference type="GeneID" id="20564492"/>
    <dbReference type="KEGG" id="ero:EROM_100620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100620"/>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_1"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6 8" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="7">
        <location>
            <begin position="48"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="358" mass="40210" checksum="3DE0C5A7ABCAFEC1" modified="2012-10-03" version="1">MKFILMNQAAELPIEFLPKNGTYRKGKLFDPKNTEIENATESDVLQDARRAAEAHRRARYKVQSIIRPGITLLEIVKSIEDSTRVLLSGERNNGIGFPAGMSMNSCAAHYSVNPEDQDIILTEDDVLKVDFGTHSNGRIMDSAFTIAFKQEFEPLLMAAKEGTETGIKSLGIDVRVCDIGRDINEVISSYEMVVDGNTWAIRPVSDLHGHSISQFKIHGGISIPPVNNKDITRITGDTFYAVETFATTGKGFINDRPPCSHFMLNTYKSRKLYNKDLMKVYEFVRDSFGTLPFSPRHLDYYNLVEGSSLKSVNLLTMMGLFIPYPPLNDIDGCRIAQFEHTIYLSENGKEVLTRGDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKV1</accession>
    <name>I6ZKV1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex protein EXO70</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265440.1">
        <property type="nucleotide sequence ID" value="XM_009267165.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKV1"/>
    <dbReference type="GeneID" id="20564558"/>
    <dbReference type="KEGG" id="ero:EROM_101280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101280"/>
    <dbReference type="HOGENOM" id="CLU_043064_0_0_1"/>
    <dbReference type="OrthoDB" id="2190236at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="496" mass="57449" checksum="B3E19E548A42C7DF" modified="2012-10-03" version="1">MGMPENPNILHKLKKCAEISREIETQANRLSSLVERLQLSVIDGFRPYINPKKNIGKLLSFYNSFILTKETIEKEKSKVIKVFGESEKRFLPRDLKMLLEKKVMESAQTLIDMSSVLKEYREVKIVGLEINSLNEFIEKTMDGISESFFGSLRRSGEYKPEFREFAAFLLSNGNRRRFMSKYTDTVYSTLGFDDIGTDIKILLERTTNLEKFLLGIIKMNNNILGKENSEVTNIGLLKMFIIGLKRAIADNLMKMEKEEDISNIPALIMLNDQLNYSGDKRIGVVEELFVFKDSIHKIICNSILRYFEDLDMMMEPNKHCGTEELCLKMKRALDAFYDHRNVSEVFVSKYGKDFELEASQDIFEKFSAKTVEKILFLAETLKGIPKSMYIINNIYIFRNYLKNIEGIPIEKMIEINLEDVLGVWKKEISKRKEEDITLFLDKNIESQGRYHLPKGLGEKLASDVEKTVNDALKNKKYKGDYGDLKNSISKLYQEPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKW9</accession>
    <name>I6ZKW9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D2</fullName>
            <shortName evidence="9">Sm-D2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_101480</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265460.1">
        <property type="nucleotide sequence ID" value="XM_009267185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKW9"/>
    <dbReference type="GeneID" id="20564578"/>
    <dbReference type="KEGG" id="ero:EROM_101480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101480"/>
    <dbReference type="HOGENOM" id="CLU_1959558_0_0_1"/>
    <dbReference type="OrthoDB" id="437526at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030532">
        <property type="term" value="C:small nuclear ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027248">
        <property type="entry name" value="Sm_D2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12777">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="23"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="128" mass="14609" checksum="FCD384DF976C59AD" modified="2012-10-03" version="1">MFLPKNLDSPNEKEEMEMKGPLSLVHRAMVKAMPVLVSLRNNRKVMGKVIAYDRHYNLLMGDAKEIGNMRGRNKGKKKRQGCDFSRRLGNVFIRGDTVILVAGGVDTFQEKGLEDNGEQRRVQNDEYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS27</accession>
    <name>I6ZS27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RING zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263898.1">
        <property type="nucleotide sequence ID" value="XM_009265623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS27"/>
    <dbReference type="GeneID" id="20520684"/>
    <dbReference type="KEGG" id="ero:EROM_010570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010570"/>
    <dbReference type="HOGENOM" id="CLU_653992_0_0_1"/>
    <dbReference type="OrthoDB" id="3838338at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044288">
        <property type="entry name" value="ZNF598/HEL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22938">
        <property type="entry name" value="ZINC FINGER PROTEIN 598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25447">
        <property type="entry name" value="RING_ZNF598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="431" mass="49845" checksum="AD5B699BDBC000C7" modified="2012-10-03" version="1">MGCQVCTGSMDVFARYDCNHRICMVCSMRLVFLYMQPTCPLCRKPAEEIIFSTLSLGCERASKQLWKGRRAASTVFYEGECVKKMMEDLLSNKCQKCHNKFGTLKELSKHYSCHGVILCLECIKNRKDFWNEIKLYRSDTIKDHKNGSLQEEGFDGHVLCVHCKIYLFDSDDAKQHCNLKHELCHVCDMMGTKYQYYSGFKDLEAHYRNAHYCCTFQTCQVNKCYVFPYQTELFEHLNRFHKVCTKLSEIPRYGKCNIPVMDPFKKKKTLARVSVMDPSGGEVRVSSFVKASIPQSKALPSTGTEIPKYLDKNILEEEKKRQNRRKAVINRMCKAEADGVEEIINEFLEGFMDVMEAFNKISEMVGDAITLKLFETIHFGAKQGTIENNIRALRKKVMFPKFIPSKPVIYGEPEERRSPGFTVIDLHKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSD7</accession>
    <name>I6ZSD7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Clathrin coat assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264018.1">
        <property type="nucleotide sequence ID" value="XM_009265743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSD7"/>
    <dbReference type="GeneID" id="20520806"/>
    <dbReference type="KEGG" id="ero:EROM_020460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020460"/>
    <dbReference type="HOGENOM" id="CLU_826469_0_0_1"/>
    <dbReference type="OrthoDB" id="2189860at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD" evidence="1">
        <location>
            <begin position="115"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="336" mass="38512" checksum="D49955D3279C6D63" modified="2012-10-03" version="1">MIEEIFIVGRGDRVLYGDPSRYVKGMKYPVDEIEGTRMLQAAVNDVKIGVLYSLVSDFSILEYISRLKSKLEKRLGEVNEKNVLENYFLLLRVVNKQESIVLFDMKKRLIPPIVSNNVYLDVCEDLNAIVDDGRVVVNRTDGRCYLNGVFGEERTVRFDVCGVKPSAIAYKNSGQGSEGLEGVRVDAKVHRGKTEAVRYSCSDSERLLIMICKADGGYVLNCQSPTKFDWLEICFPIPKMASKVVKSHSLGKSVYDEENDLLRWTFKKEVVKRARISYRVEMFEGCSDLRPIIINFCIKEWKNPKVRIERAECIGNPCVCFWIRYSMASGRYEIRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSI3</accession>
    <name>I6ZSI3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264063.1">
        <property type="nucleotide sequence ID" value="XM_009265788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSI3"/>
    <dbReference type="GeneID" id="20520852"/>
    <dbReference type="KEGG" id="ero:EROM_020910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020910"/>
    <dbReference type="HOGENOM" id="CLU_1065700_0_0_1"/>
    <dbReference type="OrthoDB" id="2191642at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="261" mass="29622" checksum="208A00BBE9AAC3A0" modified="2012-10-03" version="1">MDFVTPTKQKELRTAFQKLGTTVVVVYGPPGSSKTHSIRRTADALSLTIEYIEDVGVYRGMLPVPNTICLTDLDDYEYLSKHRSRLVKMKNLVIETRTLLSIGKILPDAVIVKFGKVSNRKILKFYNLSEEEALVVDGNLHAVEFCKYSVRNEAISIFHLLGRLFHSKETSVSKACEMANMYGRDRFFGYVMENCSFFMSISDIQRLIEGLSVDDLGGAQLERLVWIIMDSKKSSPKGFFSFRSFRGVSNEHVCNSICMNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSY3</accession>
    <name>I6ZSY3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA-intron lyase</fullName>
            <ecNumber evidence="2">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_030800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264198.1">
        <property type="nucleotide sequence ID" value="XM_009265923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSY3"/>
    <dbReference type="GeneID" id="20520991"/>
    <dbReference type="KEGG" id="ero:EROM_030800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030800"/>
    <dbReference type="HOGENOM" id="CLU_177208_0_0_1"/>
    <dbReference type="OrthoDB" id="48041at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13070">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="6">
        <location>
            <begin position="7"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="108" mass="12421" checksum="1C5C08FD237BAE46" modified="2012-10-03" version="1">MKTHQEKREALRRMFEEEGFVVGDGLKYGVDLLVYTDKPSRVHSRYGVLIDRRHSFLDIVGAQRTCTSVNKTLVVAFFEGCNVRMVSVERMELREQKKEGCGEPQGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT53</accession>
    <name>I6ZT53_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264298.1">
        <property type="nucleotide sequence ID" value="XM_009266023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT53"/>
    <dbReference type="GeneID" id="20521096"/>
    <dbReference type="KEGG" id="ero:EROM_040320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040320"/>
    <dbReference type="HOGENOM" id="CLU_1555239_0_0_1"/>
    <dbReference type="OrthoDB" id="2196338at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="172" mass="20098" checksum="EBE4D87504411235" modified="2012-10-03" version="1">MNVTKSKIDRAIDRYKGLRIGRGEYKDMVRILTEENFTNTSRSKVMELIELFISAETKPVYLNEVKNYLFENNKALYERYASMFLKNPGVFEAFGVQGEERNPAVQEDGPIEFKSLKPKLSGIGIKRKSKALRKAIHRESKMSAHHKIMEEKSASIEYQRKIDKMYRKARKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTQ7</accession>
    <name>I6ZTQ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DNA polymerase alpha/primase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_051250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264553.1">
        <property type="nucleotide sequence ID" value="XM_009266278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTQ7"/>
    <dbReference type="GeneID" id="20521357"/>
    <dbReference type="KEGG" id="ero:EROM_051250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051250"/>
    <dbReference type="HOGENOM" id="CLU_026253_2_1_1"/>
    <dbReference type="OrthoDB" id="421393at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10537:SF3">
        <property type="entry name" value="DNA PRIMASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26466">
        <property type="entry name" value="DNA_primase_lrg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0639">Primosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="8">
        <location>
            <begin position="230"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="399" mass="46467" checksum="7660EB1B1FC20A52" modified="2012-10-03" version="1">MKRIKTMKENGWPGITFYTTLSKSSVDYETFRRYAERRIEMLKGEESEGSLSKYLTVDIEDDVLSHFFCRMVCSQDPWAATWFVNAEVNLLRLRISKNPSGARSFFLTEILPRMEGGKVQDGVLILGMKSRYNPDVAGGMVSSNVLVHFTKVIDLIGKRLAVPERGYLKLNDEGIKSMLVNEYRRYLDRRMDELVEVSLYDPDERMKILSTELLTNSTRCKRNISFSLKESEKYFPLCIQIIMQRLKRNRHLKYNDRQILCLFLKDCGMNVDDGIDFFRSSFKVGREVFDKEYLYSIRHNYGLEGKRANYSCFTCSKIANTTNEERRSSCPFIEDKEYVMKLAEGMEADIEDVMDGGTFNGKCTRLLEKLTRKKQEKLVVTPIRYYLEYKSPDENAGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU11</accession>
    <name>I6ZU11_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_060370</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264628.1">
        <property type="nucleotide sequence ID" value="XM_009266353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU11"/>
    <dbReference type="GeneID" id="20521434"/>
    <dbReference type="KEGG" id="ero:EROM_060370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060370"/>
    <dbReference type="HOGENOM" id="CLU_019521_0_0_1"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990180">
        <property type="term" value="P:mitochondrial tRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07718">
        <property type="entry name" value="RNaseZ_ELAC1_ELAC2-C-term-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027794">
        <property type="entry name" value="tRNase_Z_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13691">
        <property type="entry name" value="Lactamase_B_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="11">
        <location>
            <begin position="331"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="596" mass="68134" checksum="68950132993FEBE2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU48</accession>
    <name>I6ZU48_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="9">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_060780</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. SSU72 is required for 3'-end formation of snoRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 12">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the cleavage and polyadenylation factor (CPF) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264668.1">
        <property type="nucleotide sequence ID" value="XM_009266393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU48"/>
    <dbReference type="GeneID" id="20521474"/>
    <dbReference type="KEGG" id="ero:EROM_060780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060780"/>
    <dbReference type="HOGENOM" id="CLU_062463_0_1_1"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="190" mass="22080" checksum="081DA9ED5AE3ADA1" modified="2012-10-03" version="1">MKLAVSCAMNQNRSMQTHDLFAKKGIPIKSFGTNPVIKLPGETMDKPNVYSFGQTYQQIYDDLCMKNEDYYRESGILYLLERNMRVKEKPENFFQRDEDFDLVITCEERVFTSIFEHYADGSSCTKSFFMVNFDIKDTPSDAITGAQEILEFVEDVLSLEENGLRYAVDDALRRYFERKGTMLLFTVVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU60</accession>
    <name>I6ZU60_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-complex protein 1 subunit epsilon</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CCT-epsilon</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_060940</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264683.1">
        <property type="nucleotide sequence ID" value="XM_009266408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU60"/>
    <dbReference type="GeneID" id="20521490"/>
    <dbReference type="KEGG" id="ero:EROM_060940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060940"/>
    <dbReference type="HOGENOM" id="CLU_008891_7_2_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012718">
        <property type="entry name" value="Chap_CCT_epsi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02343">
        <property type="entry name" value="chap_CCT_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="532" mass="58693" checksum="8D811688A2DB355E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUH9</accession>
    <name>I6ZUH9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Replication factor A protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264838.1">
        <property type="nucleotide sequence ID" value="XM_009266563.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUH9"/>
    <dbReference type="GeneID" id="20521650"/>
    <dbReference type="KEGG" id="ero:EROM_070900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070900"/>
    <dbReference type="HOGENOM" id="CLU_185473_0_0_1"/>
    <dbReference type="OrthoDB" id="2190827at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11778" checksum="284171113FCC347F" modified="2012-10-03" version="1">MIFAISYSIPMHPLDVENYEGQDVTIIGRFESVEDGVVILKCMDKEIQVRHRSIESYKTGIVRVNGIVENGVVVENSVFPIGNEFDMETYGRFVAVAAKYQDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV01</accession>
    <name>I6ZV01_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265073.1">
        <property type="nucleotide sequence ID" value="XM_009266798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV01"/>
    <dbReference type="GeneID" id="20521895"/>
    <dbReference type="KEGG" id="ero:EROM_081600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081600"/>
    <dbReference type="HOGENOM" id="CLU_522772_0_0_1"/>
    <dbReference type="OrthoDB" id="2190080at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="526" mass="61362" checksum="3A71048F0C2291F2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV16</accession>
    <name>I6ZV16_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA polymerase III transcription factor IIIC subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83591.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266813.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV16"/>
    <dbReference type="GeneID" id="20521910"/>
    <dbReference type="KEGG" id="ero:EROM_081750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081750"/>
    <dbReference type="HOGENOM" id="CLU_824125_0_0_1"/>
    <dbReference type="OrthoDB" id="5598268at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase III type 1 promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:RNA polymerase III type 2 promoter sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019136">
        <property type="entry name" value="TF_IIIC_su-5_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040454">
        <property type="entry name" value="TF_IIIC_Tfc1/Sfc1"/>
    </dbReference>
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        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13230">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIIC, POLYPEPTIDE 5"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF09734">
        <property type="entry name" value="Tau95"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor IIIC subunit 5 HTH" evidence="1">
        <location>
            <begin position="149"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="338" mass="39675" checksum="4E4CA41647F75C42" modified="2012-10-03" version="1">MIKDFDIVECPFREDKCVLPLIKTDSGYEMRICNKPDAKKVTATRVKERKGYFHLEITDGECKIIGYTENMYSFVVPADFYLPVMKETGEFYSEIYSKMVYGDMGQCMDVAEMFDKAYKDSEIPMYPVPIISTVESFENYNFKDFKGGKERTGDTIHVGYEDDTPEGPVPGLPERLEKLHGEDVMKLQEDLFNHLFATHPILRISNIIRMFKSDERAAEMGFSDWKIKKLLPLHAYFIDSGPWRGCWVKFGVDPKQDQSNFKYQIYDSRKSGRTFQVFEAENVVHEVERNRSWYLSDEPNFRMGFHTKALLNLLRFRYEVDFHANEEDSDELEFEVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVC6</accession>
    <name>I6ZVC6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Calmodulin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266898.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVC6"/>
    <dbReference type="GeneID" id="20564283"/>
    <dbReference type="KEGG" id="ero:EROM_090590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090590"/>
    <dbReference type="HOGENOM" id="CLU_152676_0_0_1"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF0">
        <property type="entry name" value="CALMODULIN LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="140" mass="16187" checksum="5137C7911A788817" modified="2012-10-03" version="1">MEEMKRLQKVYQLFAESPTERVPTERLGDILRFAGYIISEGYAGELMASIPGEMFDFNQLVDICERIREREITREELQKSFRRLDVDNTSHIDAGKLVQILSCGENKFTEEEIEELLNILSPDGDGKICYDLVIKNIFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVI3</accession>
    <name>I6ZVI3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265243.1">
        <property type="nucleotide sequence ID" value="XM_009266968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVI3"/>
    <dbReference type="GeneID" id="20564354"/>
    <dbReference type="KEGG" id="ero:EROM_091300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091300"/>
    <dbReference type="HOGENOM" id="CLU_442128_0_0_1"/>
    <dbReference type="OrthoDB" id="2189986at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20938:SF0">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="616" mass="71232" checksum="85EAC11959DFA4BE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVQ0</accession>
    <name>I6ZVQ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">60S ribosomal protein L23A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081890</name>
        <name evidence="8" type="ordered locus">EROM_100020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265101.1">
        <property type="nucleotide sequence ID" value="XM_009266826.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265318.1">
        <property type="nucleotide sequence ID" value="XM_009267043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVQ0"/>
    <dbReference type="GeneID" id="20521924"/>
    <dbReference type="GeneID" id="20564432"/>
    <dbReference type="KEGG" id="ero:EROM_081890"/>
    <dbReference type="KEGG" id="ero:EROM_100020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100020"/>
    <dbReference type="HOGENOM" id="CLU_037562_4_0_1"/>
    <dbReference type="OrthoDB" id="1267328at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000532">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="105" mass="11869" checksum="E829C6B987B4B090" modified="2012-10-03" version="1">MDALKKNSGHSRNLVPKVPRNTPNDIILYALTTEKAARCMDQFNTLVFYVEKTSTKPEIKKAITELYGFKVKKVNTLINKHGKKAYVRFDEKDAALEIASRIGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVQ5</accession>
    <name>I6ZVQ5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase III subunit RPC6</fullName>
            <shortName evidence="6">RNA polymerase III subunit C6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_100080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic RPC34/RPC39 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265323.1">
        <property type="nucleotide sequence ID" value="XM_009267048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVQ5"/>
    <dbReference type="GeneID" id="20564438"/>
    <dbReference type="KEGG" id="ero:EROM_100080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100080"/>
    <dbReference type="HOGENOM" id="CLU_033661_0_1_1"/>
    <dbReference type="OrthoDB" id="613763at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000116">
        <property type="entry name" value="DNA-directed RNA polymerase III subunit RPC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007832">
        <property type="entry name" value="RNA_pol_Rpc34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016049">
        <property type="entry name" value="RNA_pol_Rpc34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12780">
        <property type="entry name" value="RNA POLYMERASE III DNA DIRECTED , 39KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05158">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028763">
        <property type="entry name" value="RNA_pol_Rpc34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR028763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="235" mass="27096" checksum="DBB36B1173089277" modified="2012-10-03" version="1">MKSVLDFIRSRPEGVVEEDLSLEFPKMSKVDIARELNECLKRQEISLFRDKGVLYYKPSPVNIDDYELTIYNLVSQSGNEGVWLKVIKDKTNMPHNLIGKVLRSMESKRIIKSVKCLKNNRKVYVLYDEVPSEEITGGIWFQDNDVDAECVTRVLEIIQLFLEKRLNGNLELPEYEDNPALEEIMSYVKNLNILSVPLRAEDLETLVDILVFDGKVEKLHSGSVTRYRALRQGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVV9</accession>
    <name>I6ZVV9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Glycerol-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="3 7">1.1.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_100750</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a quinone + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:18977"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.3" evidence="3 7"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265388.1">
        <property type="nucleotide sequence ID" value="XM_009267113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVV9"/>
    <dbReference type="GeneID" id="20564505"/>
    <dbReference type="KEGG" id="ero:EROM_100750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100750"/>
    <dbReference type="HOGENOM" id="CLU_015740_3_1_1"/>
    <dbReference type="OrthoDB" id="264015at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004368">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.870">
        <property type="entry name" value="Alpha-glycerophosphate oxidase, cap domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031656">
        <property type="entry name" value="DAO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038299">
        <property type="entry name" value="DAO_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000447">
        <property type="entry name" value="G3P_DH_FAD-dep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11985:SF15">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16901">
        <property type="entry name" value="DAO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01001">
        <property type="entry name" value="FADG3PDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00977">
        <property type="entry name" value="FAD_G3PDH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="8">
        <location>
            <begin position="44"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-glycerophosphate oxidase C-terminal" evidence="9">
        <location>
            <begin position="430"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="600" mass="67259" checksum="FBEC7C75918FDD94" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVW6</accession>
    <name>I6ZVW6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265398.1">
        <property type="nucleotide sequence ID" value="XM_009267123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVW6"/>
    <dbReference type="GeneID" id="20564515"/>
    <dbReference type="KEGG" id="ero:EROM_100850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100850"/>
    <dbReference type="HOGENOM" id="CLU_655567_0_0_1"/>
    <dbReference type="OrthoDB" id="2196155at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="419" mass="49257" checksum="CF6A97C284A2E79B" modified="2012-10-03" version="1">MSRFFESADEEREENYKAKVKEKTYEESEKQSKKEKRLYDLQCKVMELEEEENQKAFDKKLKKMLTDVKKVENHFGRALPPFLKKFLMSPKVYTRSHKKAIDELLSRYEPKESIDTQKEDKNDEERISKDLSKILVIKDGEQRKKELKEFKDSTKDGTMKAKALIALLSAYMKSKDGVEIMRTIDELLDCFIDGEEDVVRSMLLENIDFYLEILYEILDSSKITPYSKLLKRLSDISRESVEGRVLQFEFFKLGRATETDHPLFKLLYIDRVQGYKESKEYYKTMGDTIGETKIEQEVLQEFGMSSFKSGDFETSFKVLSKCSGVEGSSHETLMKLLCVILNDRIRGTQIHNEFLEGFKSFGRNRFCLPSGDNTFEVYRSFYLLNMLDMKGAGDIVRRFCERFEEDIWFKDFVESRIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVZ8</accession>
    <name>I6ZVZ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">SNF2 DNA/RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_101210</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265433.1">
        <property type="nucleotide sequence ID" value="XM_009267158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVZ8"/>
    <dbReference type="GeneID" id="20564551"/>
    <dbReference type="KEGG" id="ero:EROM_101210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101210"/>
    <dbReference type="HOGENOM" id="CLU_000315_0_2_1"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="69"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="360"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="12">
        <location>
            <begin position="656"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="828" mass="97432" checksum="D0F621160C9A1677" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWA8</accession>
    <name>I6ZWA8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair protein RAD51 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_110690</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required both for recombination and for the repair of DNA damage caused by X-rays.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the RecA family. RAD51 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265548.1">
        <property type="nucleotide sequence ID" value="XM_009267273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWA8"/>
    <dbReference type="GeneID" id="20564668"/>
    <dbReference type="KEGG" id="ero:EROM_110690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110690"/>
    <dbReference type="HOGENOM" id="CLU_041732_0_2_1"/>
    <dbReference type="OrthoDB" id="10251254at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070192">
        <property type="term" value="P:chromosome organization involved in meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990426">
        <property type="term" value="P:mitotic recombination-dependent replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19513">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000008">
        <property type="entry name" value="DNA repair protein RAD51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000092">
        <property type="entry name" value="DNA repair protein Rad51 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011941">
        <property type="entry name" value="DNA_recomb/repair_Rad51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02239">
        <property type="entry name" value="recomb_RAD51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF39">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD51 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="8">
        <location>
            <begin position="92"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="9">
        <location>
            <begin position="272"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="334" mass="36536" checksum="9EA5E1114EF08AB7" modified="2012-10-03" version="1">MSEEYQYQDTEYHSESISISELKNGGILAVDIAKLIEAGFTTVESLAFAPKRQLLSIKGFSDIKVDKLIKEAAKLVPMGFTTASAYHQRRSELVYLTTGSSEVDKLLNGGFESGSITEIFGEFRTGKTQLCHTVAVTCQLPPEQGGGGGKAMYIDTEGTFRSERLVPIAERFGLDPNEVMDNISYARAYNSDHQSQLLIKASAMMSESKYSVLIIDSATALYRTDFSGRGELGARQLHLAKYLRSLVNLAETFRVAVIITNQVVSNVDGAVGMFAGDIKKPIGGNIMAHASTTRLYLRKGRGETRICKIYDSPCLPESEAVFAITEQGINDPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACR5</accession>
    <name>I7ACR5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263872.1">
        <property type="nucleotide sequence ID" value="XM_009265597.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACR5"/>
    <dbReference type="GeneID" id="20520657"/>
    <dbReference type="KEGG" id="ero:EROM_010300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010300"/>
    <dbReference type="HOGENOM" id="CLU_1151787_0_0_1"/>
    <dbReference type="OrthoDB" id="2191249at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="241" mass="28597" checksum="FB454FDD050A33B5" modified="2012-10-03" version="1">MEKSLRKVIFEEYMNYIKVESCRPKLSSSGPFFSLNRYDLSGEKVRVFSIELTGVEFYVIDGYILLYQGNMVGIYRYDKRFKCTKIRSINLHLLLFLLRKKAMSRRCRFLFYNILLHLVKNNEKKYDMKLREIMCYLCADWMTDVKLGDGVVSCTYRNGESRVYNGRLQLIKSSPSWRKDKSTMECGDQKVRVMKNCVEVTRNDWQLKSMFPVGEIRQYIALRSNLFLLTEDSIKVLSFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACS9</accession>
    <name>I7ACS9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_010460</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263887.1">
        <property type="nucleotide sequence ID" value="XM_009265612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACS9"/>
    <dbReference type="GeneID" id="20520672"/>
    <dbReference type="KEGG" id="ero:EROM_010460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010460"/>
    <dbReference type="HOGENOM" id="CLU_027280_4_0_1"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="10">
        <location>
            <begin position="345"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="414" mass="47853" checksum="EC7A35B2B4CD699D" modified="2012-10-03" version="1">MKSEVSQAWAYPIDIIEYFGMLPDHSKAQLYGAPIFAVSFLKIFDEKTTRFIFDLLLMSHSISSLRDIEGIKETLRTLLKIQMIEKKGDNIYLDASFRRSLMEGFCILKMEKHYKSTGKGLEIDENESNGKFEAILRSIVNREYRIQAFGIGEVLMFGGLLRKDRNITNRGFEFLLKTKKEQLWCLVLLSLKYFLRSVEEEIYALEALFELSTKTIETAHHQDGRMDGRLLKYLEALGILRVCGERLAIGRSFVQLFEASERNRREFIIVETNNKIYAYTNSEYEKSVIHLFCNVSVSLPNLIKGSITEESVNVAFDKGITGKQIIHFLEASVKPGSLPPAITSQIMIWESKRNRIFMVPGYIYSNFLNLSDYQRVLEFCTERSYLIESDVGKRMIVVKLEGHELVREFIKTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADM7</accession>
    <name>I7ADM7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_030490</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264167.1">
        <property type="nucleotide sequence ID" value="XM_009265892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADM7"/>
    <dbReference type="GeneID" id="20520960"/>
    <dbReference type="KEGG" id="ero:EROM_030490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030490"/>
    <dbReference type="HOGENOM" id="CLU_128308_0_0_1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 2 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="4">
        <location>
            <begin position="28"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="1">
        <location>
            <begin position="30"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="125" mass="14307" checksum="0958116A6C753623" modified="2012-10-03" version="1">MRKNDFDVREMNAALGLLKLARERKGEGDEPDIRTRKTTFQMMVLKEVFKIAPHPSTLTKTDLALMIKLPLKAVQIWFQNERSRKERGGRAGKRSRGGKSESIDPVRLLKVIMKVLDKNKEGLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADY6</accession>
    <name>I7ADY6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264312.1">
        <property type="nucleotide sequence ID" value="XM_009266037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADY6"/>
    <dbReference type="GeneID" id="20521111"/>
    <dbReference type="KEGG" id="ero:EROM_040470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040470"/>
    <dbReference type="HOGENOM" id="CLU_1835158_0_0_1"/>
    <dbReference type="OrthoDB" id="10412874at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="147" mass="17477" checksum="E6A0D47C1EFB6A81" modified="2012-10-03" version="1">MKNKASYPKGMSKTDAQGPKMMVVRIKRKDIPLHKKSNKLIYEYSVWDNKVTLLMSEGYNKVLYECKRMPRICPEEEKEFEVELGEDEALLLRTSPREPYCSGLRKNIKKKLYTARETYRLFDPATNLDFSSDECSEAFFEEWERLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE24</accession>
    <name>I7AE24_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264367.1">
        <property type="nucleotide sequence ID" value="XM_009266092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE24"/>
    <dbReference type="GeneID" id="20521167"/>
    <dbReference type="KEGG" id="ero:EROM_041030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041030"/>
    <dbReference type="HOGENOM" id="CLU_1488999_0_0_1"/>
    <dbReference type="OrthoDB" id="2189648at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="182" mass="21860" checksum="738969CB57FA2CE0" modified="2012-10-03" version="1">MKSTDFEMRLIERYGTGVIPKEEVESILNEAVESNVIERDEIGKWEKEEEYTVPRLLRLMRKWRTDFLKDSFSINDISCKDEGRYDDRCLEENELRKFEYNEDTNANDSFMTTDEAFLSSLRDKTRIITSRGSSNGKRIVRNVLKAWEEYERLPTKKRSRKWYFLGLLAVPMYFFLYVQKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE30</accession>
    <name>I7AE30_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264377.1">
        <property type="nucleotide sequence ID" value="XM_009266102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE30"/>
    <dbReference type="GeneID" id="20521177"/>
    <dbReference type="KEGG" id="ero:EROM_041140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041140"/>
    <dbReference type="HOGENOM" id="CLU_074166_0_0_1"/>
    <dbReference type="OrthoDB" id="5581259at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005344">
        <property type="entry name" value="TMEM33/Pom33"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03661">
        <property type="entry name" value="TMEM33_Pom33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="216" mass="25041" checksum="5F003295117F8AEC" modified="2012-10-03" version="1">MPQLSSTKKTWMLLNMLFGANYTLYITLHLIRIPIYPLPNFVNILCLISSYSISLLPHFSSIGEILSQPNIYCIMVFLTFPHEILLLPFYLLSIYHLSSFVLSNKKIFERTVIYPACVSLSAYHVSLGRLALFTEALTVPLSFLMIFLRKSSLVTFTAFIAMVRQQYFNNPSMRSVFGEIRVSLDRWILSCPRDVQEYYRRGRDFLVSTHSTKKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE90</accession>
    <name>I7AE90_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA mismatch repair protein MutL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_050270</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264457.1">
        <property type="nucleotide sequence ID" value="XM_009266182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE90"/>
    <dbReference type="GeneID" id="20521259"/>
    <dbReference type="KEGG" id="ero:EROM_050270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050270"/>
    <dbReference type="HOGENOM" id="CLU_004131_2_2_1"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16926">
        <property type="entry name" value="HATPase_MutL-MLH-PMS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032189">
        <property type="entry name" value="Mlh1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="MutL/Mlh/PMS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF12">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16413">
        <property type="entry name" value="Mlh1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair protein S5" evidence="6">
        <location>
            <begin position="218"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="564" mass="64785" checksum="ADFDE03BB93FED9A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEA8</accession>
    <name>I7AEA8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">TATA-binding protein-associated factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050520</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264482.1">
        <property type="nucleotide sequence ID" value="XM_009266207.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEA8"/>
    <dbReference type="GeneID" id="20521284"/>
    <dbReference type="KEGG" id="ero:EROM_050520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050520"/>
    <dbReference type="HOGENOM" id="CLU_069947_0_0_1"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08047">
        <property type="entry name" value="TAF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037817">
        <property type="entry name" value="TAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006751">
        <property type="entry name" value="TAFII55_prot_cons_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF0">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TAFII55 protein conserved region" evidence="7">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="240" mass="28049" checksum="F41E0878CB63529F" modified="2012-10-03" version="1">MERQFILRLHETIKHVVDLKSSTLDVCNDKSVVLVHNGKRYPGIIVRLPCIVESQKTMDGRQHYKVADISTLVVVYPHGDFDFDKEREMHELSGLCPPLKYVKARRFRKKNSKVEYVEEIERKVNELLEKDMKAISVEVVTKEEKEDSDDLDVLAAEIENKLADHEELHQEEVQACPPEADEKKEEKPRNAEVERLEKSIEEKRKQMESALNPILQKRFESQLGVLMKELEEMKRSLEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF84</accession>
    <name>I7AF84_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264847.1">
        <property type="nucleotide sequence ID" value="XM_009266572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF84"/>
    <dbReference type="GeneID" id="20521659"/>
    <dbReference type="KEGG" id="ero:EROM_070990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070990"/>
    <dbReference type="HOGENOM" id="CLU_943425_0_0_1"/>
    <dbReference type="OrthoDB" id="2194604at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="295" mass="34402" checksum="15F5DC5FE722745D" modified="2012-10-03" version="1">MNIYILLHLGLMASRMGIAIKSVLSGKYLNLRSLKLEPVDKRRKEDYIFRYDWDRDVPYGFRLYTREGYLTVDKRYRKMVFDKGHGRTRRGSPSRFEFHFVTPTKCKSIIIGHDSGLCMVDAGDEVRFTECMPPGKELPEFRFEIKVFKDRCGYLEKSKKVRAEGEDKSSHPEDDTKGKDEGGGPKMNQDEEKISSETTDYSEPGCHQNVEKEDKKEDEEKEDKEKEDKEKEEKKKKSHAKGNDDESLEDIIEKVRFKRPRIKKKMKRLGSSLKRKLAKAILKVDKKRDSGDSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFF6</accession>
    <name>I7AFF6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">G2/mitotic specific cyclin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_080180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264937.1">
        <property type="nucleotide sequence ID" value="XM_009266662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFF6"/>
    <dbReference type="GeneID" id="20521754"/>
    <dbReference type="KEGG" id="ero:EROM_080180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080180"/>
    <dbReference type="HOGENOM" id="CLU_020695_2_4_1"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20512">
        <property type="entry name" value="CYCLIN_CLBs_yeast_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000001">
        <property type="entry name" value="G2/mitotic-specific cyclin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046965">
        <property type="entry name" value="Cyclin_A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004367">
        <property type="entry name" value="Cyclin_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048258">
        <property type="entry name" value="Cyclins_cyclin-box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02984">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001771">
        <property type="entry name" value="Cyclin_A_B_D_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01332">
        <property type="entry name" value="Cyclin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00292">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 4" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="86"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin C-terminal" evidence="6">
        <location>
            <begin position="179"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclin-like" evidence="5">
        <location>
            <begin position="183"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="300" mass="34939" checksum="100CAB9235E9D762" modified="2012-10-03" version="1">MVVSFRKSEPLKNVTNKIGKRSEKQTFPLTKMEEVIYKHQKIAEASQIIMVDKYSDDIFEYFSEVDKPFVYINDGVSWKMRSLLVDWIIDIHDKLGLCHDTLFLAINLIDRFLSMRSIPGSKLQLVGISALMIACKYEEVVCPALQTFVLLTEKTLTGEDVRKAEKYMLHTLNYDLQYVSPLNFLRRCSKANNYEISSRAVGKYLLELMTLYQEFLRFKGSVRAAAAMYLSRKMTSQDHCKNLFLMYSGHMKDDIKECFNALVRIISESIPFENIQNKYNTPKFLHVSSFVKKYAEKNFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFL3</accession>
    <name>I7AFL3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_081090</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265022.1">
        <property type="nucleotide sequence ID" value="XM_009266747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFL3"/>
    <dbReference type="GeneID" id="20521844"/>
    <dbReference type="KEGG" id="ero:EROM_081090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081090"/>
    <dbReference type="HOGENOM" id="CLU_003041_16_9_1"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="CDD" id="cd17966">
        <property type="entry name" value="DEADc_DDX5_DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SF2_C_DEAD"/>
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        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
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        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00270">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Q_MOTIF"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="118"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="305"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="493" mass="55601" checksum="A073751EB0E43011" modified="2012-10-03" version="1">MTYRGYSGDKRGFRGPSRVDRGSRYPTRTRREPQRYEPMAELPSVEFQKNFYKEAESISKMNASEVASFRKASEMVVKGMNIPHPIHKFEDVGFPHRVVEDLVRKGFEGPTPIQAQGWPMALSGRDMVGIAQTGSGKTLSFILPALVHAKDQPPLRRGDGPIVLVLAPTRELVMQIKKVADEFCEMFDLRSTAVYGGASSQPQIRALHEGAEVVIATPGRLIDLHEQGHAPLGRVTFLVLDEADRMLDMGFEPQLRKIIPKTNPNRQTLMWSATWPKEVRGLAESYMNDYIQVVIGNEELKTNSKIKQVIEVCNGRDKEDKLLGVLDKFKGDRIIVFCNMKRTCDDLEYVLNRSGYGAAALHGDKSQNIRDKVLDDFRSGRRPILIATEVAGRGLDVNDIKLVINFDFPGSCEDYVHRIGRTARGNTKEGISHTFFTINDKGNARELIRMLKEAKQVVPSDLEDMVRASNDRYGSRGSRYDYRGRASRFSYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFU3</accession>
    <name>I7AFU3_ENCRO</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="ero:EROM_081960"/>
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    <dbReference type="HOGENOM" id="CLU_1142588_0_0_1"/>
    <dbReference type="OrthoDB" id="2193208at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="253" mass="29917" checksum="B3D9E6E961B05FA8" modified="2012-10-03" version="1">MDINILILSLLMQESYSIAIEAIRSSREPALLLATLILHSINNLLNMVKDPKMVPFFLYGSALQHLREKNFDGFWREVDFGIKDARKEINALSTHVTNWQWDNFDTWFMARTSRFDELQDFNRDVYSLYLDELECVVKKNFYGDTNRLWQNLRDLTTFSRERTSRYILDYLMNTLGNKVIIAQRSYSFIKPLSDGRSRKVYVVFLNDFIKRPDETMDDIINGTRNLSRESIAWHCKLYGLYWLLTLVTTGNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFU7</accession>
    <name>I7AFU7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_082010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265112.1">
        <property type="nucleotide sequence ID" value="XM_009266837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFU7"/>
    <dbReference type="GeneID" id="20521936"/>
    <dbReference type="KEGG" id="ero:EROM_082010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_082010"/>
    <dbReference type="HOGENOM" id="CLU_172741_0_0_1"/>
    <dbReference type="OrthoDB" id="2193106at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="102" mass="11967" checksum="38EE3DDBDDC168CE" modified="2012-10-03" version="1">MEEVVYFNDGKTEVNKVLDENPSYNKVYMKITRKDNVTDIILSREKGDILYILSSVEKEKYSDYFKNKTEDEATTINNEKKMKGGPIPKRTKQSTLFDFIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG87</accession>
    <name>I7AG87_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265267.1">
        <property type="nucleotide sequence ID" value="XM_009266992.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG87"/>
    <dbReference type="GeneID" id="20564378"/>
    <dbReference type="KEGG" id="ero:EROM_091540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091540"/>
    <dbReference type="HOGENOM" id="CLU_503453_0_0_1"/>
    <dbReference type="OrthoDB" id="2200771at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012468">
        <property type="entry name" value="DUF1686"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07937">
        <property type="entry name" value="DUF1686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="499"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="541" mass="60241" checksum="B2C9855D4929DFB5" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGP3</accession>
    <name>I7AGP3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_101350</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Gln) + L-glutamine + ATP = L-glutaminyl-tRNA(Gln) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265447.1">
        <property type="nucleotide sequence ID" value="XM_009267172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGP3"/>
    <dbReference type="GeneID" id="20564565"/>
    <dbReference type="KEGG" id="ero:EROM_101350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101350"/>
    <dbReference type="HOGENOM" id="CLU_001882_2_3_1"/>
    <dbReference type="OrthoDB" id="10250478at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.800.10:FF:000001">
        <property type="entry name" value="Glutamine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000037">
        <property type="entry name" value="Glutamine--tRNA ligase cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF4">
        <property type="entry name" value="GLUTAMINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="10">
        <location>
            <begin position="197"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="11">
        <location>
            <begin position="512"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="12">
        <location>
            <begin position="607"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="697" mass="80946" checksum="A202B6A5F1E393DC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGV1</accession>
    <name>I7AGV1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metal-dependent protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_110430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265522.1">
        <property type="nucleotide sequence ID" value="XM_009267247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGV1"/>
    <dbReference type="MEROPS" id="M67.A10"/>
    <dbReference type="GeneID" id="20564642"/>
    <dbReference type="KEGG" id="ero:EROM_110430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110430"/>
    <dbReference type="HOGENOM" id="CLU_052991_0_0_1"/>
    <dbReference type="OrthoDB" id="605656at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000026">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056263">
        <property type="entry name" value="RPN11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23594">
        <property type="entry name" value="RPN11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="7">
        <location>
            <begin position="21"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="299" mass="33846" checksum="2CA9FBDA5A27F38E" modified="2012-10-03" version="1">MFSGFVGAGDAEPMPDTSETVQISSLALLKMLKHGRAGIPLEVMGLMLGEFVDEYTVKVVDVFAMPQSGTNVTVESVDPIFQMEMMSILKATGRHETVVGWYHSHPGFGCWLSTVDISTQQSFEKLCKRAVAVVVDPIQSVKGKVVIDAFRLIDNQLGVLGGEPRQVTSNIGYLKTPTLISIIHGLNKHYYSFNITCRKNDLEQKMLLNLHRKTWADNLKLRNIKKERENVLKLIESYGKACKEERNLTGKNLDMAKIGKVDYRRRLLEKCEENIMENTIYNLLYSIHRYVFNQSDHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH34</accession>
    <name>I7AH34_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_111390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265617.1">
        <property type="nucleotide sequence ID" value="XM_009267342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH34"/>
    <dbReference type="GeneID" id="20564738"/>
    <dbReference type="KEGG" id="ero:EROM_111390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111390"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000407">
        <property type="term" value="C:phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF22">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="287" mass="33298" checksum="9B2A63E226BF07B1" modified="2012-10-03" version="1">MERFRFIRKLSRGVHGTVYLLETIDSGKEKMACKSILWKYKKYAEREVKILSLISHKRIVKLLDFFPRDSGIFIILEYVNCGSLHEMISHLVENNYRAPNCLVWSVLVQIADGLRYLHNKHIAHRDIKPSNILIERVFVQSGALIEFKLCDFAMAKELYEGNRTCEIAGTPSYMAPEVVSREYYNISIDIWSLGVSVYELLTLKKPFEGRSRDELFGMIKQARLPQVISPDIELERLIRRCLSKNDRISSGAIFNDKKAKAHLEVSEAELGGVRRFLKMKDRRNIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALE6</accession>
    <name>I7ALE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263991.1">
        <property type="nucleotide sequence ID" value="XM_009265716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALE6"/>
    <dbReference type="GeneID" id="20520777"/>
    <dbReference type="KEGG" id="ero:EROM_020190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020190"/>
    <dbReference type="HOGENOM" id="CLU_1875412_0_0_1"/>
    <dbReference type="OrthoDB" id="2188968at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707841" evidence="1">
        <location>
            <begin position="25"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="136" mass="15720" checksum="72179A6C617BE65B" modified="2012-10-03" version="1">MIHIYLFLAGAFCGGSILISEVNSKTDTFKFNNMILNIEKSGAFEKNISHEPNRPGYVVRGEKNKFQIEFFTSMTDLRVSRMGPNKIKVVYNSKDRSTNDTYLEISIKNRTIKNFKIFDFYMLKKAQLSSKLDIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALZ1</accession>
    <name>I7ALZ1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YIP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_030480</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the YIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264166.1">
        <property type="nucleotide sequence ID" value="XM_009265891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALZ1"/>
    <dbReference type="GeneID" id="20520959"/>
    <dbReference type="KEGG" id="ero:EROM_030480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030480"/>
    <dbReference type="HOGENOM" id="CLU_074741_4_2_1"/>
    <dbReference type="OrthoDB" id="440385at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048280">
        <property type="term" value="P:vesicle fusion with Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045231">
        <property type="entry name" value="Yip1/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236">
        <property type="entry name" value="GOLGI MEMBRANE PROTEIN YIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21236:SF2">
        <property type="entry name" value="PROTEIN YIPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Yip1" evidence="7">
        <location>
            <begin position="64"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22186" checksum="EAA2463EEDDC68FD" modified="2012-10-03" version="1">MEFEECGEGYTGKLDLKSAFLGKLPGDGPLLEELGIDFSTIKKESRLIFGVFQKKHVDFSFVKNADLSGPIVFIGLYTLGLILNYKIHFGYVYFISLLTALSMYFLLNVLDTKHIGFLECCSVLGYSFLPVVFFSFINILMGRIGVGFKVFYGLVFALWSSYTASVVFCRYLSLSNKHVVVGYPLLLGYAGFVMVVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM46</accession>
    <name>I7AM46_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">tRNA nucleotidyltransferase/poly(A) polymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_031320</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264251.1">
        <property type="nucleotide sequence ID" value="XM_009265976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM46"/>
    <dbReference type="GeneID" id="20521046"/>
    <dbReference type="KEGG" id="ero:EROM_031320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031320"/>
    <dbReference type="HOGENOM" id="CLU_019592_0_1_1"/>
    <dbReference type="OrthoDB" id="445712at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CC tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3090.10">
        <property type="entry name" value="cca-adding enzyme, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734:SF5">
        <property type="entry name" value="CCA TRNA NUCLEOTIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13734">
        <property type="entry name" value="TRNA-NUCLEOTIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81891">
        <property type="entry name" value="Poly A polymerase C-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly A polymerase head" evidence="6">
        <location>
            <begin position="31"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase/poly(A) polymerase RNA and SrmB- binding" evidence="7">
        <location>
            <begin position="192"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="464" mass="53722" checksum="FDDADEB0CE9BC226" modified="2012-10-03" version="1">MDNQINCTEAEEDIFDLIIRHAKEFGDVVPRVAGGWVRDKIMGNPSFDMDIALENISGYKFAAGLVKSATGQKLTEVHVIKDNPDKSKHLETAVVRINGFCVDFVNLRNETYSETRIPNINPGTPQEDAFRRDLTINSLFYNLFTRRIEDYTGRGIDDIKGKILRTPLDPRTTLFDDPLRLVRIFRFHSKFGFKIDEKIDEALLDERIKTALGKKVSNERIHIEIFKILHYPRGQYGLLEIIRRGYVNPIFKPPVQRETSYEKGIVFCEIIEKITNLWGRPYRREVLNLYTVLCFFRGSTIPSQKGTIFSNTYIMKKSLPSSKKFVKMIDKIEESLDFLSRCDMKRLGSEDLIRMVRFMGEMWYESLVVYSAIEYMEGRKEKYQDGLRAVYSISQEDIGECYLSKAIVDTRKLAQTLGIPKADTKFYIEESIVYQLLSGVKAPEEIIGHLRSIKTVRDTHQKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM62</accession>
    <name>I7AM62_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264271.1">
        <property type="nucleotide sequence ID" value="XM_009265996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM62"/>
    <dbReference type="GeneID" id="20521066"/>
    <dbReference type="KEGG" id="ero:EROM_040020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040020"/>
    <dbReference type="HOGENOM" id="CLU_2399676_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="93" mass="10934" checksum="6225538D68830A09" modified="2012-10-03" version="1">MGERRFYLVEFKGPVSMEWVKHAPHPKLRKTFTELLHEKEGEHEVNSSMIKEGVFIVECMSLEEALVDCIKEKRKGQSLSSRGRFTWYRTSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM91</accession>
    <name>I7AM91_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein SA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_040400</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264306.1">
        <property type="nucleotide sequence ID" value="XM_009266031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM91"/>
    <dbReference type="GeneID" id="20521104"/>
    <dbReference type="KEGG" id="ero:EROM_040400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040400"/>
    <dbReference type="HOGENOM" id="CLU_058171_2_0_1"/>
    <dbReference type="OrthoDB" id="414863at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_uS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_uS2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_uS2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_uS2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN SA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="Ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="252" mass="27892" checksum="F95348BAA3DA96C5" modified="2012-10-03" version="1">MPQDSTKISDSIKIPDEFVKLLILSQAHLGGTSTNKNFSRYMYGTRSQDKISIIDINAMWEKLIIAARAFCGIKHPSSIAVVSTKTFGRKPVVKFCEAVGATPITGRFIPGSFTNSEVRRVYDPRLLIVSDTYADKQAILESQYCNLPTIAFVNADNSLIGVDIAIPMNNRSPSAIAAGFFILSRLINYMKTGAELVRDMKEVELFLFRDSVELEQLVEEQLLESTNNILNVGKEEVLSGIAGGNTEEWNGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMC0</accession>
    <name>I7AMC0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleolar protein 58</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_040760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for pre-18S rRNA processing. May bind microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264341.1">
        <property type="nucleotide sequence ID" value="XM_009266066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMC0"/>
    <dbReference type="GeneID" id="20521140"/>
    <dbReference type="KEGG" id="ero:EROM_040760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040760"/>
    <dbReference type="HOGENOM" id="CLU_015495_1_0_1"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000005">
        <property type="entry name" value="Nucleolar protein 5, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047099">
        <property type="entry name" value="Nop5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF1">
        <property type="entry name" value="NUCLEOLAR PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="7">
        <location>
            <begin position="262"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="413" mass="46977" checksum="6BC142DD61E06EFF" modified="2012-10-03" version="1">MIYLVETVVGYALLDKAERSEEVQLIEKYKFASIDEALESSALLLRGEIPGNLENFVRLVEDKSKDTLVVSDQKLVEPLEKMFSRKVVCSHDDVLRSIKLDVHKYFDMSWAEYSERILCLAHKISMEKINLVPEKIDTMVIQSVSLLDDMDKDINLHCMRLKEWYGFHFPELSSITDNNREYLKLVVAIGRKGSIDEEKKRMLRDMVGDDCEKIVKLAETSMGVAMDEEDVLNILEDAKGVLRSFEFRDELVEYIRVKMEGLAPNITSLVGEVIGAKMISKAGSLSNLARMPGSSIQMMGAEKALFQALKSKTNTPKYGIIYGCSLLGQVASQNKGRIARSLASKIAMAARIDSYGEERTNKVGIRMREKIERRIKDLETRSNSEKKVAKKLKYEVKPDFYDDSKDSKRIKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN78</accession>
    <name>I7AN78_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MYB-like transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_060970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="KEGG" id="ero:EROM_060970"/>
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    <dbReference type="HOGENOM" id="CLU_1081943_0_0_1"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019185">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001006">
        <property type="term" value="F:RNA polymerase III type 3 promoter sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042795">
        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042796">
        <property type="term" value="P:snRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
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    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46621:SF1">
        <property type="entry name" value="SNRNA-ACTIVATING PROTEIN COMPLEX SUBUNIT 4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="153"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="158"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="158"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="7">
        <location>
            <begin position="201"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="201"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="6">
        <location>
            <begin position="206"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="257" mass="30565" checksum="6318FC2570A4ADE3" modified="2012-10-03" version="1">MLEKGALDKRLEKLYMDYIEIEKILKRLRDIKKQCSYRSTVIKPKIKGTGSLFVDADMKLDEDFQKATKNCMRFDLNDPFWKEISIRFNIPRNECFNRWINKVEDFDKKLCKSDMDTVKHMSDEGKDWIQIAKEMGCKPLKVFAEYLRLNSISTPKMWSLEEDRLLEKGVEKYGLSKWRYVSKIVGTRTGKECAMRFYFLNKNVQKGKWTKDEKERLFEGVEIYGEGDWRGVSTHVMTRNPIQCRSKYVAETRKINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANA4</accession>
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            <fullName evidence="3">CDT1 Geminin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_061380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83229.1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="ero:EROM_061380"/>
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    <dbReference type="HOGENOM" id="CLU_733671_0_0_1"/>
    <dbReference type="OrthoDB" id="2192570at2759"/>
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        <property type="component" value="Chromosome VI"/>
    </dbReference>
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        <property type="entry name" value="CDT1_Gemini-bd-like"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08839">
        <property type="entry name" value="CDT1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CDT1 Geminin-binding" evidence="3">
        <location>
            <begin position="118"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="374" mass="44260" checksum="9A0994E475E9FCF3" modified="2012-10-03" version="1">MGEHEIRRKRLLRRKKEVSRESIKAGGCKMEKEQDHGKVVLIGRWLEKLKELGLNGEENVITRKKDADEEARVSEKDREETTEDKGGLNEERKEKIRQFGGVSHLEFKGLSLNFHLYLPKSLQKLSRIFRVLETIQRFNSARNLTTIFIKSLGCIENLVKQRVVVGDIERMYYIAPDLFRFKKISIHHDGKEVDTFVIYVSGETKEFNERLEEYVKKCHLEFLNENGYVYNGNRFHPEFNFEEVPLPRKPLFPEDIKEETIEKEVCVPTKRNVDIVESAKKRASTILERIREKERLRREEFMNKSKEEEDNVLLSNRIKMLLRSENRRAIPVNKVVDLLKIFDGVNTINRIIKWDSSLFYIRNMRGVEYLVSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AND0</accession>
    <name>I7AND0_ENCRO</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264761.1">
        <property type="nucleotide sequence ID" value="XM_009266486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AND0"/>
    <dbReference type="GeneID" id="20521571"/>
    <dbReference type="KEGG" id="ero:EROM_070130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070130"/>
    <dbReference type="HOGENOM" id="CLU_507350_0_0_1"/>
    <dbReference type="OrthoDB" id="2193826at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031540">
        <property type="entry name" value="DUF5088"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17008">
        <property type="entry name" value="DUF5088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="602" mass="70031" checksum="3C8213C91018BACA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7APA2</accession>
    <name>I7APA2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_090210</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265136.1">
        <property type="nucleotide sequence ID" value="XM_009266861.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APA2"/>
    <dbReference type="GeneID" id="20521960"/>
    <dbReference type="KEGG" id="ero:EROM_090210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090210"/>
    <dbReference type="HOGENOM" id="CLU_027721_6_0_1"/>
    <dbReference type="OrthoDB" id="331948at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12246">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="69"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="265" mass="30990" checksum="B48DDC4649DF97B4" modified="2012-10-03" version="1">MVLLISSLMLTYTVPVSEIILPTRFAFKRAYLSLLLLSSIFSIIYLALSIVYRGHVRYDLEAISLANHHARKFCKRCNNYKPERAHHCSSCGHCVKKMDHHCFWINNCVNYDNQGHFIRFLFFTSLANLLIFTYVCGEFVAILLFDRHLRHKRDYYILVLSGMSSLVFLIMTPFFLYLQLRLALMNMTFIEELKQEDISRHQGSPFLRSPYDRGVINNLIDTLGPPYTLFLMGPFGDGITFVKTYSTEHWPVPYDYHCIEDLMVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7APB9</accession>
    <name>I7APB9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265161.1">
        <property type="nucleotide sequence ID" value="XM_009266886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APB9"/>
    <dbReference type="GeneID" id="20521986"/>
    <dbReference type="KEGG" id="ero:EROM_090470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090470"/>
    <dbReference type="HOGENOM" id="CLU_044236_1_0_1"/>
    <dbReference type="OrthoDB" id="266138at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15454">
        <property type="entry name" value="NISCHARIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25090" checksum="F4C2B37422988913" modified="2012-10-03" version="1">MGDTVYIVHRQLERIPTIPEHARTVDLRRNNISRMVLNKAELVEYLDLSDNRIREISDLENVPRLKILDLSYNLISDILIPRMDIEELYLISNDISTIRGLDLPRIKKLDLAVNDICRIENLGNCITLEELYLGSNRIRAVEGLEDLTSLKILDLQNNELEVVDCSMIPSSVEVLLLGENRDLRVVEKTEHLPRLRILGLEKTSVPKDLIEGGFTIWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7APH5</accession>
    <name>I7APH5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265236.1">
        <property type="nucleotide sequence ID" value="XM_009266961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APH5"/>
    <dbReference type="GeneID" id="20564347"/>
    <dbReference type="KEGG" id="ero:EROM_091230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091230"/>
    <dbReference type="HOGENOM" id="CLU_115515_0_0_1"/>
    <dbReference type="OrthoDB" id="437922at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF0">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="2">
        <location>
            <begin position="29"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="210" mass="23418" checksum="0B57E3A28921779A" modified="2012-10-03" version="1">MIVSEEGFREDGRRPRELRRVEVVRRMVGKKGVVDVRQGNTQVSVMCGGAKEAGKLDIEVEFYAVSRQEPVNEKRILEYEDVLLDIFSDILLPETSVGIKVVVREDGGSLLSTMVNCITLCLSYFGIPLMDMCYSATIGEHCIDLTSTEESQKIPVITVAYLMNRKSIAYLSLNGRIVCDKLQSSILEGVDACYSVGEHFKGFFSFSPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7APL8</accession>
    <name>I7APL8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chromosome segregation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_091950</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265306.1">
        <property type="nucleotide sequence ID" value="XM_009267031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APL8"/>
    <dbReference type="GeneID" id="20564419"/>
    <dbReference type="KEGG" id="ero:EROM_091950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091950"/>
    <dbReference type="HOGENOM" id="CLU_001042_5_0_1"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="3">
        <location>
            <begin position="438"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="174"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="591"/>
            <end position="649"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1017" mass="119096" checksum="2D379B7D5ECADBFE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7APZ9</accession>
    <name>I7APZ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265451.1">
        <property type="nucleotide sequence ID" value="XM_009267176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APZ9"/>
    <dbReference type="GeneID" id="20564569"/>
    <dbReference type="KEGG" id="ero:EROM_101390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101390"/>
    <dbReference type="HOGENOM" id="CLU_581436_0_0_1"/>
    <dbReference type="OrthoDB" id="2194238at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="471" mass="52494" checksum="0676B5F5FFC9FBDB" modified="2012-10-03" version="1">MDESSDKGKGKESISNEKVEEDRMLMVNEESNAENRECLGSNVMKHVIGAACMLSHKSSSEDIVERSEEKVSTFDVYIVNENGKVNGYELKHISPIDGPETEDPPRDIQVDEMMKSFHFDRKSKGVPPHEFYRRRRSSELPRNNDGNNSSGAKEKCDPIILEISEISDTYNTDGMNENQDDSTSNQSTTLNKDCSQQGEEVLTPSIVLSDSTGQESFPRFNREDLTASECSRERNCVVLLNESNTAFKTSGIVSSETKNGSPTNSETEVVTLPDISKAIKLESEEKKDMEANSCEDMGDIIKAIQAQEPSDKLSINQKIGLNSPYDSAGKKCQCETMRGSESLSKELKTALLAAFVPSMEPSKAETAEASETSEMSFVETSITRNINENMDTTGEIERFRAVLNLGSAANNPTYSDGEKKPKEEDKDNEMIVFVEAEDIYKKKSSTLTRNNKVGRLVKFFESLEERNKEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQJ0</accession>
    <name>I7AQJ0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphotransferase</fullName>
            <ecNumber evidence="6">2.7.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-fructose + ATP = D-fructose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37721"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16126"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-hexose + ATP = a D-hexose 6-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22740"/>
            <dbReference type="ChEBI" id="CHEBI:4194"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:229467"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:22741"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the hexokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265621.1">
        <property type="nucleotide sequence ID" value="XM_009267346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQJ0"/>
    <dbReference type="GeneID" id="20564742"/>
    <dbReference type="KEGG" id="ero:EROM_111430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111430"/>
    <dbReference type="HOGENOM" id="CLU_041825_0_0_1"/>
    <dbReference type="OrthoDB" id="419537at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008865">
        <property type="term" value="F:fructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001678">
        <property type="term" value="P:intracellular glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24000">
        <property type="entry name" value="ASKHA_NBD_HK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.367.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001312">
        <property type="entry name" value="Hexokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022672">
        <property type="entry name" value="Hexokinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443">
        <property type="entry name" value="HEXOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19443:SF16">
        <property type="entry name" value="HEXOKINASE TYPE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00349">
        <property type="entry name" value="Hexokinase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00475">
        <property type="entry name" value="HEXOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51748">
        <property type="entry name" value="HEXOKINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Hexokinase N-terminal" evidence="7">
        <location>
            <begin position="35"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="458" mass="50987" checksum="081AD6F666B80A99" modified="2012-10-03" version="1">MFCILKRVEVVVVLIAGIFGMWSSVDGKQTTNKVTSFPIQDFEIQEIKKAFSERIAKAQEASSDGDFLRSHIDLIKSEVEHPRCSSFLVIDVGGTHLKIGEILIDFSFRKCGVNPLCFDGVIECSPSFYKYPDTSKIPNENRITWNEWVAEKIAEFYGQEPPKSGIDGCLTLSYPLLQTSINHATIERVTKNFCFKVDESTFGVNVVEALNSSLRDRNVNVHVNCVVNDSTATYMAGVLRGYNNIIGIVLGTGTNSSFCVKKKGSEEMVLYNSEWGSTNVPRSILTEADLAVITDLETRNISYNIIDVLAGGCKLNDIIFNRLKNLHPEVYEKYAGEEEVLRSMIHSAITEPSKSIFGEDIQLHRILISMINHFNTRGIKILGALSSAIIDSCMKDVQEVTLILNGMAFSNRKLREALEVEMKNAHPNIDVNLVFFGNASLEGSAFVSSMYSNDTNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQJ8</accession>
    <name>I7AQJ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Spindle pole body associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_111480</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265626.1">
        <property type="nucleotide sequence ID" value="XM_009267351.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQJ8"/>
    <dbReference type="GeneID" id="20564747"/>
    <dbReference type="KEGG" id="ero:EROM_111480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111480"/>
    <dbReference type="HOGENOM" id="CLU_1053847_0_0_1"/>
    <dbReference type="OrthoDB" id="2190654at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043495">
        <property type="term" value="F:protein-membrane adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045119">
        <property type="entry name" value="SUN1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911:SF8">
        <property type="entry name" value="KLAROID PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12911">
        <property type="entry name" value="SAD1/UNC-84-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51469">
        <property type="entry name" value="SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="SUN" evidence="6">
        <location>
            <begin position="99"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="264" mass="30262" checksum="394636F1A1B27929" modified="2012-10-03" version="1">MNRRDRLQVKRSPDSTLNFGIDETIMLQTPTPKMRATKSVEPVEAHKYGQGGKGVKVYPVYVAIGILYVILFYIAITRPMNSMIIMNLMEEISILREENSRMSKQIESMGYTKEVNYAKVEEGARIRIENVSQLFPYGLLGFRKYKDPATIFDENVSVGECLSFKGCNCKFFIDFDKEITIDRVGFYHPRTKDTSSAIHEFEVFCYGSNGRLSLGEFKYDVDACGFQTFELEKVKTNSIEFAIRSNGGNKKFTCIYKVYLFGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQK3</accession>
    <name>I7AQK3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inorganic phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265631.1">
        <property type="nucleotide sequence ID" value="XM_009267356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQK3"/>
    <dbReference type="GeneID" id="20564752"/>
    <dbReference type="KEGG" id="ero:EROM_111530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111530"/>
    <dbReference type="HOGENOM" id="CLU_105537_0_0_1"/>
    <dbReference type="OrthoDB" id="2190094at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20483" checksum="3BD0A4F14726B207" modified="2012-10-03" version="1">MGLSVVSVDQFVNFALSIVSMKVSGKPMFQTPKAIWILRGAYLCSNLIQLVFSLYIMQKIKSTNDSRKVKIKREATLFKDNNEEEEEEMTYASYDESELTKVSRTAVIQFLIITVLHLKWKVIQPLFVQSFVPIRNLLLNPLYTAYVWNKPVLRPFEANMLFQKAPAVSEKKRVKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQL9</accession>
    <name>I7AQL9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_021420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264114.1">
        <property type="nucleotide sequence ID" value="XM_009265839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQL9"/>
    <dbReference type="GeneID" id="20520904"/>
    <dbReference type="KEGG" id="ero:EROM_021420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021420"/>
    <dbReference type="HOGENOM" id="CLU_783093_0_0_1"/>
    <dbReference type="OrthoDB" id="2193268at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="376" mass="44053" checksum="51614D6E404F8632" modified="2012-10-03" version="1">MFILGMQNMAKLFPLHSQIAKMKDRIQEIKYTDEMDYIVEGLDNDEIDESLRLVIDSITDERFKTYMKESRHLEEMIQIMSRKKISVSASNMILEILFQVFNPYSFVDFEGLFRILMVIFDGLLMEGTLFDASSCIKPKIKSIIELLGRIPIKWYPGLSKSIELKKIVFILQICMSKRIVKWNEFLLSLLMECPEFELSEDFIRILLRKGRNYEILANYTTLEANVGRLSLSPSFKECLKKAVLREFVKSKGPRILGYMKRESSSYAVPSIPRHKREEMRILNRRKYLMIILINLISRNFCLDIPLDLIVRAYHTVDEVTRCYMALFLLIFVQDSEKNSIQNAYIEDLKKNARCLIFDCSAYLNEDVLSKIVKKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQV8</accession>
    <name>I7AQV8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264189.1">
        <property type="nucleotide sequence ID" value="XM_009265914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQV8"/>
    <dbReference type="GeneID" id="20520982"/>
    <dbReference type="KEGG" id="ero:EROM_030710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030710"/>
    <dbReference type="HOGENOM" id="CLU_1327278_0_0_1"/>
    <dbReference type="OrthoDB" id="2189917at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031537">
        <property type="entry name" value="DUF5092"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17010">
        <property type="entry name" value="DUF5092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="233" mass="27137" checksum="EC56D645DAC78D26" modified="2012-10-03" version="1">MNDRSSKLKQVLDRQIYCLARRNTVMAYLLEKGNGSRCVLEAIKVEPFTQKQFADMIKEAHAKSQDYYLARVQCRSNPEDGKNIYYCYDARQLCKYIFEMVISTEGRKIRIKNFKDPINQEQIHEISFFKLRYDSDTPLKAEYVGNQVNFLENNCFRSKIFYQENALDALSVNFQFGPQKKKIPIINRRKLFTVFMMVILVFVIGTLVAMVAEREPSKASPPLKLSINKGKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQW7</accession>
    <name>I7AQW7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ser/Thr protein kinase Kin1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_030860</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264204.1">
        <property type="nucleotide sequence ID" value="XM_009265929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQW7"/>
    <dbReference type="GeneID" id="20520997"/>
    <dbReference type="KEGG" id="ero:EROM_030860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030860"/>
    <dbReference type="HOGENOM" id="CLU_000288_59_11_1"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="58"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="566" mass="65611" checksum="13C54684DC15AA6A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR26</accession>
    <name>I7AR26_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">sn-1-specific diacylglycerol lipase</fullName>
            <ecNumber evidence="14">3.1.1.116</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_040280</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33275"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17389"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.116"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:33276"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.1.116" evidence="14"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264294.1">
        <property type="nucleotide sequence ID" value="XM_009266019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR26"/>
    <dbReference type="GeneID" id="20521092"/>
    <dbReference type="KEGG" id="ero:EROM_040280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040280"/>
    <dbReference type="HOGENOM" id="CLU_025519_0_0_1"/>
    <dbReference type="OrthoDB" id="438440at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016298">
        <property type="term" value="F:lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046340">
        <property type="term" value="P:diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052214">
        <property type="entry name" value="DAG_Lipase-Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792">
        <property type="entry name" value="DIACYLGLYCEROL LIPASE HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45792:SF7">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_6G02710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fungal lipase-type" evidence="15">
        <location>
            <begin position="352"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00026104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN82797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="595" mass="68283" checksum="D6047B316EB7F0F8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR48</accession>
    <name>I7AR48_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_040540</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264319.1">
        <property type="nucleotide sequence ID" value="XM_009266044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR48"/>
    <dbReference type="GeneID" id="20521118"/>
    <dbReference type="KEGG" id="ero:EROM_040540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040540"/>
    <dbReference type="HOGENOM" id="CLU_1137987_0_0_1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45977">
        <property type="entry name" value="TARGET OF ERK KINASE MPK-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="61"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="133"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="14">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="246" mass="28832" checksum="8CF1E6B2B3BBBE76" modified="2012-10-03" version="1">MYVREGEEDFVNSQTQERTNALKFLKTLIVMEGFLKVLKILVTLTVLFLTRNEKCEVPLKLFLLVYMVITVAKFGIFMSKNIPFFRINRIPEYRENTDITLFSNFIEALLLFWYLIGFNWVQECEDCSTTNPLLYYTTVVFVGLGFVAFIAPLIAIVLLLFLITFVKPKLQEVMYKDQNDVSDDTYHCTICFDNYIPGIKLKLLPCGHHFHQECIDEWLDLKDTCPLCKRNINLLYDLIDPPEYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARR7</accession>
    <name>I7ARR7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264569.1">
        <property type="nucleotide sequence ID" value="XM_009266294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARR7"/>
    <dbReference type="GeneID" id="20521373"/>
    <dbReference type="KEGG" id="ero:EROM_051420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051420"/>
    <dbReference type="HOGENOM" id="CLU_090468_1_0_1"/>
    <dbReference type="OrthoDB" id="10249667at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048248">
        <property type="entry name" value="PUA_eIF2d-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26292">
        <property type="entry name" value="PUA_elF2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="81"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="18938" checksum="B0BD2F90163C7AF2" modified="2012-10-03" version="1">MKNLFNKVEISSSCCLGKKDKKDINKRLGYELLEKNEAYMIHRCKNKVSLVSLGGVAILFFFNRKYFPTIRYLEKHDSEFHEIFLDDGALGPLCRGADVMIPGILKYKDLIKKEFEAGDTVVIRIVGKSIVAIGEAVISLQEMVSKGNGVGVEVYHRIGDELYCGEWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS58</accession>
    <name>I7AS58_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_061460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264734.1">
        <property type="nucleotide sequence ID" value="XM_009266459.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS58"/>
    <dbReference type="GeneID" id="20521543"/>
    <dbReference type="KEGG" id="ero:EROM_061460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061460"/>
    <dbReference type="HOGENOM" id="CLU_439466_0_0_1"/>
    <dbReference type="OrthoDB" id="2196204at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22453">
        <property type="entry name" value="KH-I_MUG60_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="392"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="598" mass="68499" checksum="5AB44F9C4ED83D8A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS62</accession>
    <name>I7AS62_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ER lumen protein-retaining receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_061560</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ERD2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264744.1">
        <property type="nucleotide sequence ID" value="XM_009266469.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS62"/>
    <dbReference type="GeneID" id="20521553"/>
    <dbReference type="KEGG" id="ero:EROM_061560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061560"/>
    <dbReference type="HOGENOM" id="CLU_057784_0_1_1"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00952">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10 11 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="238" mass="27513" checksum="D33E69695A68766D" modified="2012-10-03" version="1">MFLSTIIADTFRFCGDFLHVASKVVLITKIARTRSCSGLSLKTQFLYAMVFIFRYVDLFDFYIKGSLGVYNFIMKILFIGFQILILFMMRFKYFSTYDKKWDKFNVLVLIVPCFAFSLFFARGGGGVYRYTSNVLYISSLFLESVAILPQLVQLQEAGESETMTSKYILLLGLYRASYTIYFIVKRIYDVKNIGNIPIACGIVQTLLYMDFFAIYYRYVFRKSGISESLPSSNIKGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS93</accession>
    <name>I7AS93_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">H/ACA ribonucleoprotein complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Nucleolar protein family A member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_070410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Common component of the spliceosome and rRNA processing machinery.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Required for ribosome biogenesis. Part of a complex which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264789.1">
        <property type="nucleotide sequence ID" value="XM_009266514.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS93"/>
    <dbReference type="GeneID" id="20521600"/>
    <dbReference type="KEGG" id="ero:EROM_070410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070410"/>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_1"/>
    <dbReference type="OrthoDB" id="1924699at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002415">
        <property type="entry name" value="H/ACA_rnp_Nhp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00883">
        <property type="entry name" value="NUCLEARHMG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="7">
        <location>
            <begin position="17"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="120" mass="13213" checksum="EF48ADABB6F7FA88" modified="2012-10-03" version="1">MTATQTYSPVSDEKAQNILRLIQNATRSRNIRKGINESTKCLNKGRALLVVIACDAEPQEITAFLPIICEDKGVPFVHVPSKSALGVACGIHRPIAACTIYLPKEAESLRLEEKIREALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASD6</accession>
    <name>I7ASD6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-directed RNA polymerase I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264839.1">
        <property type="nucleotide sequence ID" value="XM_009266564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASD6"/>
    <dbReference type="GeneID" id="20521651"/>
    <dbReference type="KEGG" id="ero:EROM_070910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070910"/>
    <dbReference type="HOGENOM" id="CLU_058320_0_1_1"/>
    <dbReference type="OrthoDB" id="248779at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1340.10">
        <property type="entry name" value="RNA polymerase, Rpb5, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036710">
        <property type="entry name" value="RNA_pol_Rpb5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000747">
        <property type="entry name" value="RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53036">
        <property type="entry name" value="Eukaryotic RPB5 N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="2">
        <location>
            <begin position="135"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="210" mass="24267" checksum="BC66E582B3C883D4" modified="2012-10-03" version="1">MKLQKSSPTEMALELRALCLSRNTTIEMIKDRGYTTTQEVQSLESFHERYPFALTKRSTLNFVCVDVDRPVGIHFGDDEKMGKKGFEGLLSQYEKDNIFHLILILPQFPSPTVMSMISLNPKFNVEIFLVEEMVFNKTKHSWVPPHRILSREEKENVLKALKLGPGDLPRILKSDVIARYYGAKRGDVMEIVRRSRTAGESIYYRIVVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASL2</accession>
    <name>I7ASL2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264924.1">
        <property type="nucleotide sequence ID" value="XM_009266649.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASL2"/>
    <dbReference type="GeneID" id="20521740"/>
    <dbReference type="KEGG" id="ero:EROM_080040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080040"/>
    <dbReference type="HOGENOM" id="CLU_1326362_0_0_1"/>
    <dbReference type="OrthoDB" id="2189452at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="207" mass="23359" checksum="A863EFFA92D1F45F" modified="2012-10-03" version="1">MGNIRLSPLLHSTLMFMKKFSSRITVSKTSICSKSILVEMPEFCDCCFTVRIKDFGAMLKQTQEFVLEDRALRYGYEVGMGTSLVKVEKRIKVYDETYQITGGIPLLSIVTNGFKVLSSDEIEIRAEKNGRLVLESFGVIRTRSEYTGLRVPEHKVDLVVVKVRGKDLKVLEEFKGEIVFSFLDSHILAYSLGDDFTVIVQIGVLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASS0</accession>
    <name>I7ASS0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-directed RNA polymerase II fourth largest subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_080730</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264989.1">
        <property type="nucleotide sequence ID" value="XM_009266714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASS0"/>
    <dbReference type="GeneID" id="20521809"/>
    <dbReference type="KEGG" id="ero:EROM_080730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080730"/>
    <dbReference type="HOGENOM" id="CLU_110332_4_0_1"/>
    <dbReference type="OrthoDB" id="2186918at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006590">
        <property type="entry name" value="RNA_pol_Rpb4/RPC9_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045222">
        <property type="entry name" value="Rpb4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21297">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00657">
        <property type="entry name" value="RPOL4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb4/RPC9 core" evidence="4">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="11623" checksum="3F18A01CF69AC434" modified="2012-10-03" version="1">MLSEARYLLEGQKERFRADFRSNASKIFRSTLGYLDEFCRIKDKSVAEDLRTTFSGLGFSELEIALLGSLFPQSVEEAKTLIPSLASKSDDAIGQAVEKIQQLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASX8</accession>
    <name>I7ASX8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Longevity assurance protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_081360</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sphingosine N-acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265049.1">
        <property type="nucleotide sequence ID" value="XM_009266774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASX8"/>
    <dbReference type="GeneID" id="20521871"/>
    <dbReference type="KEGG" id="ero:EROM_081360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081360"/>
    <dbReference type="HOGENOM" id="CLU_028277_5_1_1"/>
    <dbReference type="OrthoDB" id="537032at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560:SF0">
        <property type="entry name" value="LD18904P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12560">
        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00724">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="TLC" evidence="8">
        <location>
            <begin position="67"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="289" mass="33974" checksum="DC61003AA4CC012C" modified="2012-10-03" version="1">MMTGLPDVTIDSERVFESQFEFQRDVFFVMVFCLLIIMIKNLVILPMASSLIRKFGVEEALGFERKKKFSVSLWKAMFYSFTSIYGYLIIRSEPLAYTMKNLSGTWGLHNIPFKVLFYYYLEFAYYFVELFYLFNEHMYKDFLQMVTHHIVTIMLLTLSYHRDLLRPGVIIIAVHDISDPFLEISKLTNYIHYKSLAKGIFMCFAGVFVVSRLVIYAFFISLPISISIWRYTFDLYLFLISILLQGLTVMHIIWSSMIMKMVIKVGHKEEFEDIRSVKPQGSSSDVKCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASZ8</accession>
    <name>I7ASZ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exosome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081610</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5">Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265074.1">
        <property type="nucleotide sequence ID" value="XM_009266799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASZ8"/>
    <dbReference type="GeneID" id="20521896"/>
    <dbReference type="KEGG" id="ero:EROM_081610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081610"/>
    <dbReference type="HOGENOM" id="CLU_043216_3_0_1"/>
    <dbReference type="OrthoDB" id="10253092at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF1">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="6">
        <location>
            <begin position="12"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="223" mass="25927" checksum="CA11A9BA7A82A5A9" modified="2012-10-03" version="1">MFTPLNQKKLVNVSIVALKKFGRRYELGVYPNKLYEYRNGMATPLNEILLTDTIYRSVSKGEIAGQDDLDLFCKSHEEIVREILNNGYEQKNEATRIYEQEKTEREIIEFLRNKVTKGGRHLNEASLKEAIGKVHNIYIGNSKKQSQEILGKLEKMGFDRVGIKVSVDMNDKVMEFAKKNGEVYDGYVIIRSECFPKFKEVCEGERVKYLILRREEPEDEEIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATB7</accession>
    <name>I7ATB7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear pore protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_090500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus envelope</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the nucleoporin interacting component (NIC) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265164.1">
        <property type="nucleotide sequence ID" value="XM_009266889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATB7"/>
    <dbReference type="GeneID" id="20521989"/>
    <dbReference type="KEGG" id="ero:EROM_090500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090500"/>
    <dbReference type="HOGENOM" id="CLU_021169_0_0_1"/>
    <dbReference type="OrthoDB" id="203824at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007231">
        <property type="entry name" value="Nucleoporin_int_Nup93/Nic96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225:SF4">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11225">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP93 NUCLEOPORIN NUP93 DEAD EYE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04097">
        <property type="entry name" value="Nic96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="603" mass="70223" checksum="1EA8D381B222C7A4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATK0</accession>
    <name>I7ATK0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265289.1">
        <property type="nucleotide sequence ID" value="XM_009267014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATK0"/>
    <dbReference type="GeneID" id="20564400"/>
    <dbReference type="KEGG" id="ero:EROM_091760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091760"/>
    <dbReference type="HOGENOM" id="CLU_158768_0_0_1"/>
    <dbReference type="OrthoDB" id="2191090at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="15224" checksum="94DBF5BC0B79FD28" modified="2012-10-03" version="1">MQVNTWPAPPRFKKKVPPKIPSSYVSFGTSYKVENSVPINTSFPSMKFDKDRFKELVNLSFSAFIELLAFPLDHEELIEIISSTHLEINQILNGGKGMEAISEIRRIRNDHIRNKNRIAEETRRKISYFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATK9</accession>
    <name>I7ATK9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">26S proteasome regulatory subunit 8 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_091870</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265299.1">
        <property type="nucleotide sequence ID" value="XM_009267024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATK9"/>
    <dbReference type="GeneID" id="20564411"/>
    <dbReference type="KEGG" id="ero:EROM_091870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091870"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_1_1"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="168"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00069286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="392" mass="44250" checksum="969E122CD40DE7BA" modified="2012-10-03" version="1">MAEGFCKNFYNNKLDHLEVLIAKKAKVLRVLEQKRAELNRRVRFLREEISIVQEPSSNVGIVVEKMGKMQVLVKTNPDGKYLVKVEHGVNYDDLRPGVRVALRSDSYDVHRILPTKVDPLVSLMMVEKVPDSTYQMIGGLDEQIKEIREVIELPIKHPELFENLGIAQPKGVLLYGPPGTGKTLLARAVAHHTQCKFIRVSGSELVQKYIGEGSRLVRELFVMAREHAPSIIFMDEIDSIGSTRGDSNKGSDSEVQRTMLELLNQLDGFESHNNIKVIMATNRIDILDPALLRTGRIDRKIEFPQPKESARLEILKIHSRKMNLTKGIDLEIIASKMVGCSGAEVKAVCTEAGMYALRERRIHVTQEDFEMAVHKVLKKAGDLNSDLRKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATP7</accession>
    <name>I7ATP7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265349.1">
        <property type="nucleotide sequence ID" value="XM_009267074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATP7"/>
    <dbReference type="GeneID" id="20564466"/>
    <dbReference type="KEGG" id="ero:EROM_100360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100360"/>
    <dbReference type="HOGENOM" id="CLU_1085972_0_0_1"/>
    <dbReference type="OrthoDB" id="66510at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019193">
        <property type="entry name" value="UBQ-conj_enz_E2-bd_prot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09814">
        <property type="entry name" value="HECT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="259" mass="29913" checksum="80E749FE00C287C7" modified="2012-10-03" version="1">MLELPPDTRKGFLYLTNDEVFSAFKGCIGLDFLYMENQEGVKIMAVSVMENSLLWSEDPDVEEALPDAFRCLHGNEMTYKSLNRLPQEGWEELIDCWSCHNCEFRTMLSLSPHPREGGLLLSNFFFLINDSDLPKCCRNNNSSVRKLFYNEVLQYGCTHEALAYSYLSSYFQNRSVLLLEVSQTRCEIRYFYKTMVITIENKTLCKKEAMKVGVKETDKPIEKNENINEFYSKLIYNIVVSGVIGATALGYRISLVERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATW5</accession>
    <name>I7ATW5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MdlB-type mitochondrial ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_101120</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265424.1">
        <property type="nucleotide sequence ID" value="XM_009267149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATW5"/>
    <dbReference type="GeneID" id="20564542"/>
    <dbReference type="KEGG" id="ero:EROM_101120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101120"/>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_1"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034040">
        <property type="term" value="F:ATPase-coupled lipid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF654">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="16"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="335"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="561" mass="64928" checksum="B05A011789E0D512" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU59</accession>
    <name>I7AU59_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265514.1">
        <property type="nucleotide sequence ID" value="XM_009267239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU59"/>
    <dbReference type="GeneID" id="20564634"/>
    <dbReference type="KEGG" id="ero:EROM_110350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110350"/>
    <dbReference type="HOGENOM" id="CLU_1540531_0_0_1"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="204" mass="24526" checksum="767EE4C204C4E893" modified="2012-10-03" version="1">MDFDEETLFLGNIPDSFGNELLMEMLSIFGEIKEFKRGRCLEGISSKWVIVNYHEPASVGYLYDVLFDRDLGQGTITICPPKKRSEPWEGRKNALKRIEDIERRHRKELEKEERLDRFVSSYNLLYGKADDSKMFYDTMKKWLEEESEIRKKCRRIEKDFEENIEKKRQAEYHYLSTYDDDRSNDLFYIDRASWISKRNKNGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU79</accession>
    <name>I7AU79_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265534.1">
        <property type="nucleotide sequence ID" value="XM_009267259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU79"/>
    <dbReference type="GeneID" id="20564654"/>
    <dbReference type="KEGG" id="ero:EROM_110550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110550"/>
    <dbReference type="HOGENOM" id="CLU_071075_0_0_1"/>
    <dbReference type="OrthoDB" id="5421607at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031545">
        <property type="entry name" value="SRP72_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17004">
        <property type="entry name" value="SRP_TPR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="335" mass="38910" checksum="6377639AD43CAD80" modified="2012-10-03" version="1">MNLYDLVKNDCHEEISRLEHPEFSRFRAVAFISMGKFKEALKYTEEGSFERAYVFYKLKKYKKALRILRHIDTEGSKVLTSQCLYYLGYYNTAYQILAEVKRDDDIVVNLQAMKGLATLADSNQYVFGNKFQLRKRDELAEFEDLSKHNFKHIEGYVDFIFNLAFESLSSEEDFVDFLSHQLEKQEMKGTIVEEQLKNIKGEEVNINILLKNQKETVEFNSGAIDRFSNPLHFQQNFPGIDTTGSTSKGNVSLWIEKVYNSNFNIKAEKIPLLSPKMVLLRILTLCKNGCLPSQKLLEKTKKWPESSIKNCLSVFDSKANLSKELYIRMSKDIMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGW3</accession>
    <name>I6ZGW3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">General negative regulator of transcription</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263925.1">
        <property type="nucleotide sequence ID" value="XM_009265650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGW3"/>
    <dbReference type="GeneID" id="20520711"/>
    <dbReference type="KEGG" id="ero:EROM_010840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010840"/>
    <dbReference type="HOGENOM" id="CLU_130565_0_0_1"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="1">
        <location>
            <begin position="53"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="161" mass="19366" checksum="DCC02E27BC62C99E" modified="2012-10-03" version="1">MVNIQKKLEQIQQDHHAVHDMNKDKLAEILSIVREKRYQDPSTYYPQSLLSRMLPNCYPKAPNEGIFKVNIEDMKMDNLHEETLFYIFYTFPNDKLQVKAYDNILRRKYIFCRMYKCFVTFNAPPIADHVKRSIVMFDPFSWSKVSIEVVFDERFIRSLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH01</accession>
    <name>I6ZH01_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_011240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263965.1">
        <property type="nucleotide sequence ID" value="XM_009265690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH01"/>
    <dbReference type="GeneID" id="20520751"/>
    <dbReference type="KEGG" id="ero:EROM_011240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011240"/>
    <dbReference type="HOGENOM" id="CLU_125119_0_0_1"/>
    <dbReference type="OrthoDB" id="19419at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143113">
        <property type="entry name" value="NAP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="174" mass="20766" checksum="AC855E9136BD0061" modified="2012-10-03" version="1">MIETEVYKDLEEIQWEIDRKYEEFSKKDFECKLDHHKMIKPLLIRRDQAIAKMDSGSYWGRVLDRYEIGEMILPRDSSNLINTGWMRSLSVDYLDGYAYRVEMEVNENEFVANKTLMKKAYLLEGDVERSGVRWKGNRKCPVFEFFDTDTVDLDIFDILYEVYVNSASYFLRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH20</accession>
    <name>I6ZH20_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263985.1">
        <property type="nucleotide sequence ID" value="XM_009265710.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH20"/>
    <dbReference type="GeneID" id="20520771"/>
    <dbReference type="KEGG" id="ero:EROM_020130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020130"/>
    <dbReference type="HOGENOM" id="CLU_1885743_0_0_1"/>
    <dbReference type="OrthoDB" id="2191630at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15438" checksum="EF0A3C9A9730B175" modified="2012-10-03" version="1">MGNKPGLLPKWLFIYTIIKTIYILVVHKDHLNVASSWDSGSVYAYLALLLGIARLLASRKATRVGFYSSIAGSFLAEGVFFIYSGLRGLYSVQRVIVETFLVLFTVGWMIFLYPYYLLEDNDSQEKLKVKYVKHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH57</accession>
    <name>I6ZH57_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">60S ribosomal protein L11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_020530</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264025.1">
        <property type="nucleotide sequence ID" value="XM_009265750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH57"/>
    <dbReference type="GeneID" id="20520813"/>
    <dbReference type="KEGG" id="ero:EROM_020530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020530"/>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_1"/>
    <dbReference type="OrthoDB" id="1734943at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="6">
        <location>
            <begin position="7"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="7">
        <location>
            <begin position="64"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="173" mass="19707" checksum="761A27B892D24FE0" modified="2012-10-03" version="1">MSLKKTNPMREIRIKKLSIHICARESGPKLDRAAKVLEQLTGQKPVTSKARMTIRNFGIRRNEKIAVHVNVSGKKAYELLNTALRVKEYELKEGCFSNNGCFGFGIEEHIDLGLKYDPAIGIFGMDFFVVLSRPGDRVSKRKRCKSRVGNKQRVYAEDAKKWFVESFEGVLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZII5</accession>
    <name>I6ZII5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA polymerase</fullName>
            <ecNumber evidence="5">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_051020</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264530.1">
        <property type="nucleotide sequence ID" value="XM_009266255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZII5"/>
    <dbReference type="GeneID" id="20521334"/>
    <dbReference type="KEGG" id="ero:EROM_051020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051020"/>
    <dbReference type="HOGENOM" id="CLU_345439_0_0_1"/>
    <dbReference type="OrthoDB" id="6755010at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45861:SF1">
        <property type="entry name" value="DNA POLYMERASE ALPHA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5 7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="6">
        <location>
            <begin position="356"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="947" mass="108127" checksum="C39201D026577F29" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIP3</accession>
    <name>I6ZIP3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264590.1">
        <property type="nucleotide sequence ID" value="XM_009266315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIP3"/>
    <dbReference type="GeneID" id="20521395"/>
    <dbReference type="KEGG" id="ero:EROM_051630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051630"/>
    <dbReference type="HOGENOM" id="CLU_1532534_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="175" mass="19773" checksum="DD58B678048FABB7" modified="2012-10-03" version="1">MMRLFNSSKLLSLFPAASPSLATFSFSDSCSKINSLHSSCALSLTCFSKHSFMNSAISGFSTYSIIRQILCALTLMFLYTFPDTLLTLRGIAPLSPPKSPMIPITLIASIIFRSQLYLYISFQLFLIWKSLLKLLIINFQVLYIRMFKKIFVFNNFTQINLFFGIITSILGGNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIR5</accession>
    <name>I6ZIR5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Queuine tRNA-ribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264615.1">
        <property type="nucleotide sequence ID" value="XM_009266340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIR5"/>
    <dbReference type="GeneID" id="20521420"/>
    <dbReference type="KEGG" id="ero:EROM_060230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060230"/>
    <dbReference type="HOGENOM" id="CLU_022060_1_0_1"/>
    <dbReference type="OrthoDB" id="10249838at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008479">
        <property type="term" value="F:tRNA-guanosine(34) queuine transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43530:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE CATALYTIC SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="tRNA-guanine(15) transglycosylase-like" evidence="2">
        <location>
            <begin position="14"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="389" mass="44022" checksum="630747F1CFFD6C28" modified="2012-10-03" version="1">MIGDFKVIKKCSTTNARVSKLELNDVCLELPIFMPVGTYGAMKGIRVSDLQEKMILANTYHLRALGKGIKKFMKYRGGMLTDSGGFQIGSLPDVVVTEEGVKFGATLFTPEDSMDIQMKLGADIVMQLDDVVNPCDTKEKIEIAMRRSIRWMDRCINAINSHGVNGHNKKVKREMNVPQIKLDEKSTQILFPIIQGGLDEELRAESIAEVLKRSPKGLAIGGLSGGEEKSEFARVVHFCIQNLPENIPRYLMGVGYPEDIVVCAALGSDMSDCVYPTRTARFGKALSDTGDILFTSKLKNDKRRIDESCDCYVCSKYSRAFIFSIKGTTNFCMLLSVHNLHYMRNLTRRIRESIMEDKYPEFIKTFMRRRFREIPEWIRIALRKVGVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIX1</accession>
    <name>I6ZIX1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">T-complex protein 1 subunit zeta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_060440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264635.1">
        <property type="nucleotide sequence ID" value="XM_009266360.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIX1"/>
    <dbReference type="GeneID" id="20521441"/>
    <dbReference type="KEGG" id="ero:EROM_060440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060440"/>
    <dbReference type="HOGENOM" id="CLU_008891_3_1_1"/>
    <dbReference type="OrthoDB" id="10052040at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="343" mass="38375" checksum="114B5680B7F32897" modified="2012-10-03" version="1">MNSTHSEAQVTQFGQAIRINNNTAATLSGLFSSSIGPFGSYKALISPGQTLRIAKDGNTLCKEIQFTHPTSIIITRAATSMYTTFGDGATTLIILCCEIFNDAFRHFENGVPIPRICASLQLCLDDVMRYLKTLERPFEEDTLSKMAYSIIRTKVDEDMALSLSMVLVQAVENAIQSQFFDINMVEVIKMQEGDVSETMYVDGLVLDHGGRHYAMPDKLEEVCILITNMSLEYEKPEINAEFCYSSAEQREELAGKEREFILQRSRAIAEFGRKIKEKNGKTLMVVTEKGIDPYSLEVFASSGILALRRAKRRNLERLVRMCGGNVITHVGSSMRGFWDAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIY5</accession>
    <name>I6ZIY5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Adenylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060590</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the adenylate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264650.1">
        <property type="nucleotide sequence ID" value="XM_009266375.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIY5"/>
    <dbReference type="GeneID" id="20521456"/>
    <dbReference type="KEGG" id="ero:EROM_060590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060590"/>
    <dbReference type="HOGENOM" id="CLU_032354_4_2_1"/>
    <dbReference type="OrthoDB" id="439792at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019205">
        <property type="term" value="F:nucleobase-containing compound kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01428">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00235">
        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000850">
        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033690">
        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="NUCLEOTIDE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00406">
        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="136" mass="15601" checksum="58129CC00CEF4FEE" modified="2012-10-03" version="1">MKKNSKEAEIVREVVNSGRLAPDELVNKLVLKKIRSMDKYILDGYPRRIEQAEMLGDDVDLVIFIDVDEDVSVRRICGRNQGRDDDVEEVARKRHEVYLGETAPVVELYRKQGKLLTVNGHDSPETVFSEICRSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJH9</accession>
    <name>I6ZJH9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_071470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264895.1">
        <property type="nucleotide sequence ID" value="XM_009266620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJH9"/>
    <dbReference type="GeneID" id="20521711"/>
    <dbReference type="KEGG" id="ero:EROM_071470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071470"/>
    <dbReference type="HOGENOM" id="CLU_1845085_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707099" evidence="2">
        <location>
            <begin position="27"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="139" mass="15550" checksum="71958D9626760024" modified="2012-10-03" version="1">MKMIHKIGMVRAIYLILIYKHAIILCGEDESTVEIVLLNNGTEKEKSSNGGVKGPATENKYRELKNILTTLVSHFLAGSRFKNNLLKNYDGADQPSVPKEGNDKQKPTDHRKDEGVGNLSPQEPTIEMLEKIRKNPHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJT2</accession>
    <name>I6ZJT2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Heat shock transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_080980</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265010.1">
        <property type="nucleotide sequence ID" value="XM_009266735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJT2"/>
    <dbReference type="GeneID" id="20521832"/>
    <dbReference type="KEGG" id="ero:EROM_080980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080980"/>
    <dbReference type="HOGENOM" id="CLU_008776_3_1_1"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="7">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="289" mass="33379" checksum="987D1CD053EA2253" modified="2012-10-03" version="1">MKLTMGSEFVVKLFQILEEPENYPYISWALDGRSFIISNIQGFSEFVLEKHFRHKNWSSFVRQLNKYDFYKVRRDEERIGERGQWEYKNKYFQRGRPELLDKIRRKKAPSEGSGSGSTSPKQIESGMVYQSHVLNAVRTISKYLQTVVEDINDIKKYIYHERMRIASKINVLFVKGDVPGSEWICSALKIPNYSLTIVEESSDVRNVMRWKYDVVVLYASTSGCSKSVSRIRSLDASVPIVLVVDEEFKNEYMNILGIRVSEALLMPLDPKDLVDAVNRHGSQRGLEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJV2</accession>
    <name>I6ZJV2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265035.1">
        <property type="nucleotide sequence ID" value="XM_009266760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJV2"/>
    <dbReference type="GeneID" id="20521857"/>
    <dbReference type="KEGG" id="ero:EROM_081220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081220"/>
    <dbReference type="HOGENOM" id="CLU_577581_0_0_1"/>
    <dbReference type="OrthoDB" id="2191243at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="446" mass="51246" checksum="CAA8DAC040406FF4" modified="2012-10-03" version="1">MGARRNRMRGRSRIQGLGKGTIREVKEIKKEEKQEGKFNVDFQELNNELSTIELKDESTKHSDGSEASCEKDSCMNDRDDKAEEINDKEVVDENGRDVEEEGKIGSRQENEVDVVGMSQILHPMTPNVLTNAVLQTPVRRVLHEEGNNLLVKEEGLMLPSKMIINRYPGYLFIHIDQVERFMTSRKEVESVSIKIESKGILCETKRYPVNTNVGMNEMLRVPISKVESTGTPLRFILLLHHKSGHVTKSCEAMLIIDSSRIRSIHNNLLESRLAWDNYVSRNLLKSIRSFFTSGAPDAWSLKIYLSFISEDEFKLIPAPLPQDLKSLSKWLVVKKFSYNIWFRGFVNVRGDAGKACTSLWKRRYVRCYGYMIFVFNEHSRSLIGTISLVDACFDPSTSNKVYLENFLRINLGRSSVEFHFDTKDKYNTCKNALSMMLPQSLFYNKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJZ4</accession>
    <name>I6ZJZ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265080.1">
        <property type="nucleotide sequence ID" value="XM_009266805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJZ4"/>
    <dbReference type="GeneID" id="20521902"/>
    <dbReference type="KEGG" id="ero:EROM_081670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081670"/>
    <dbReference type="HOGENOM" id="CLU_1938137_0_0_1"/>
    <dbReference type="OrthoDB" id="2196044at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="130" mass="15730" checksum="2803B78547DC02C1" modified="2012-10-03" version="1">MEKIDLEDVDDAILREKLFVKAASKFLKTLEFKKVEEEYDGELMRSQKEIEQGEKDARRKAEKMMKRHNEMRLKRKSKMSEMDSDDLEPESFIAPMLSNRETRKKNKGKHPVNKSLKRRKMKKRQKRHRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK60</accession>
    <name>I6ZK60_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265130.1">
        <property type="nucleotide sequence ID" value="XM_009266855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK60"/>
    <dbReference type="GeneID" id="20521954"/>
    <dbReference type="KEGG" id="ero:EROM_090150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090150"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_2_1"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001462">
        <property type="entry name" value="Small GTP-binding protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22708" checksum="3D8CFC6550B629CB" modified="2012-10-03" version="1">MRSTYKIVFLGSSNVGKTTLMSQYLHQEVQRSYGPTIGLDFVSTTIVVSGHRVRLQLWDTAGQERFNSIIPNYTRNSFLAIIVFDMKDKSSFERIDHWINTLSKTSESLGSKARILVVGNKRDLADEETRGWYKEVGTRKAKKHGGEYVETCAMKHEGIEDLVRAVDRLIEEDLENPMEDESTVRPENIRFVKGRRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKE4</accession>
    <name>I6ZKE4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Signal peptidase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091370</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265250.1">
        <property type="nucleotide sequence ID" value="XM_009266975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKE4"/>
    <dbReference type="GeneID" id="20564361"/>
    <dbReference type="KEGG" id="ero:EROM_091370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091370"/>
    <dbReference type="HOGENOM" id="CLU_142364_0_0_1"/>
    <dbReference type="OrthoDB" id="10261524at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007653">
        <property type="entry name" value="SPC3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04573">
        <property type="entry name" value="SPC22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17421" checksum="5AF01053074C6129" modified="2012-10-03" version="1">MKTAGRRLNALVSFTFNPTVILLLAVFLFSFFVAKAPPVSDLRIREYKAYGRTQVVFFEPSIDLSSQFHFNLKQIFVYVRVIYGGKADKSEIIWSRIIGWNDSKKLIGVFRSTYDIAGDLSDNPCFELRGCYFPRVGWIRDILFCKTDVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKF2</accession>
    <name>I6ZKF2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265260.1">
        <property type="nucleotide sequence ID" value="XM_009266985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKF2"/>
    <dbReference type="GeneID" id="20564371"/>
    <dbReference type="KEGG" id="ero:EROM_091470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091470"/>
    <dbReference type="HOGENOM" id="CLU_1427970_0_0_1"/>
    <dbReference type="OrthoDB" id="2192373at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="190" mass="22275" checksum="30B0F33A55CA5546" modified="2012-10-03" version="1">MASQRILDAVDLINNLLKTLPMHPKTMRCHGLFIPREMVGSLRKKALENTLPILERIIDDIRSTQEKHRRIDSNEICNRREFATNQLRKLRLSMENEFEENRVNEIKSLLSEIEYYEKIGVRLIDKVSIGKEVALNIFRTLSHSREGPTDPYIGEYLKKRVSFSHEFPSDIEVSSLKAMLRFLQENMDCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKH1</accession>
    <name>I6ZKH1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265285.1">
        <property type="nucleotide sequence ID" value="XM_009267010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKH1"/>
    <dbReference type="GeneID" id="20564396"/>
    <dbReference type="KEGG" id="ero:EROM_091720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091720"/>
    <dbReference type="HOGENOM" id="CLU_665686_0_0_1"/>
    <dbReference type="OrthoDB" id="2195960at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="343" mass="39106" checksum="E61AE8125960AFED" modified="2012-10-03" version="1">MSWDEYDEEESAVSSTQSQENLGGIQDESHNPNESECKVSGSCSCDKNMEDELKTKDGRAELHLTASGEVFFYLLRFNGFDLVLIKKVRKKVSSQIFVKDLYVVSDKKLYGFYDVISTPGSLDDLLKPERKRKKANGSLLSDESHEEYIYRPCRKNGKIILKREEGKSDIVSNWEGFFITSGLAHKIVYEDGRYNFVYNSKKYSYEKVIGGIAYNNGMLVYYLKGKDSSLYFQGDHEKIYNIPKITVMSCEGQRTIVGTADGYIYLFDRCVLSKRRKVYDVPITGVGFMNDQVYFSSLDGFVGTEKTTRGFSMFLIMVSIGIFIFAIVMGIISKTVRHSFLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKR1</accession>
    <name>I6ZKR1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L27A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_100870</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265400.1">
        <property type="nucleotide sequence ID" value="XM_009267125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKR1"/>
    <dbReference type="GeneID" id="20564517"/>
    <dbReference type="KEGG" id="ero:EROM_100870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100870"/>
    <dbReference type="HOGENOM" id="CLU_109163_1_0_1"/>
    <dbReference type="OrthoDB" id="61900at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="5">
        <location>
            <begin position="63"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="137" mass="15837" checksum="8B964EA4CF617184" modified="2012-10-03" version="1">MRGHVSHGYGRVGKHRKHSGGRGLAGGFSHMKTFFTRFHPDYHGKRGMRVYHRKENSNYARPISSARLWGVIPKEQRYEFLDNSDKVPVIDVREFGYHVVIGGKLPLERPIVVKARYFTPSAKEEIERVGGKWIITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKU1</accession>
    <name>I6ZKU1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIP18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265430.1">
        <property type="nucleotide sequence ID" value="XM_009267155.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKU1"/>
    <dbReference type="GeneID" id="20564548"/>
    <dbReference type="KEGG" id="ero:EROM_101180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101180"/>
    <dbReference type="HOGENOM" id="CLU_075876_4_1_1"/>
    <dbReference type="OrthoDB" id="2746at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039796">
        <property type="entry name" value="MIP18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377:SF0">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12377">
        <property type="entry name" value="CYTOSOLIC IRON-SULFUR ASSEMBLY COMPONENT 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="160" mass="18240" checksum="9508A4146A7E7921" modified="2012-10-03" version="1">MNESPFIVNSLEERYPLEISNGTLQEVTPHTVFELIRDIRDPEHPYTLEQLGVVSKERISIGLINSDDATLSIGLPIKYVRVMFKPTIPHCSMAAVIGLCIKAQLSRYIKNHFIQVHIINDGHVNFKALNKQLDDKDRVLAAMENETLFDLMKECLPIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKX7</accession>
    <name>I6ZKX7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphopyruvate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_101580</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="3"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265470.1">
        <property type="nucleotide sequence ID" value="XM_009267195.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKX7"/>
    <dbReference type="GeneID" id="20564588"/>
    <dbReference type="KEGG" id="ero:EROM_101580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101580"/>
    <dbReference type="HOGENOM" id="CLU_031223_0_0_1"/>
    <dbReference type="OrthoDB" id="1739814at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="12">
        <location>
            <begin position="17"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="11">
        <location>
            <begin position="153"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="376"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="400"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="422" mass="46890" checksum="E304651675E725F6" modified="2012-10-03" version="1">MATKSQTKQRMKIKDALLSIKSRMILTSRGYPTVEVDLITGKGVYRSSCPSGASRGSKEAVVISDGGNLYNGRGVRAVINNINDISRSLLELEYNAADQQNIDNFLLDLDGTNNKSKIGGNGIIALSTAFCKMGAGYSNKRVDEFISSIGPFKRKMPIPHFNVLNGGVHSGNAMTVQEIMVVYQHNDLERNIECGCVLYESLKKTISEKYGVLHTSVGDEGGFAPPIEKLEDGLDLILEASRRCNRTDMKIAIDFAANEFTRDDGKYELDGEVYTTRDLGEKYMEVIRKYPQIYSLEDPFSEKDYDGWRWINAKVGDKINIVGDDLTATNPQLVENAGLRKMCNTLLVKPNQIGTISETIKAIKIARKYGMKIMVSHRSGETEDHFISDLSVGVGAEYMKSGAPCRGERVSKYNQLLRLNEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL28</accession>
    <name>I6ZL28_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">USP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265525.1">
        <property type="nucleotide sequence ID" value="XM_009267250.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL28"/>
    <dbReference type="GeneID" id="20564645"/>
    <dbReference type="KEGG" id="ero:EROM_110460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110460"/>
    <dbReference type="HOGENOM" id="CLU_888600_0_0_1"/>
    <dbReference type="OrthoDB" id="2191051at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="36570" checksum="4CD65ADF7B0627A1" modified="2012-10-03" version="1">MKVKAESCVFFVNGMTAEEEYRVATKQAPKPRKRPVGRLNGSKIMLSGQKAFVKRPMRVVDLKYIESDEKMFNLLACLIRLPPFYNFILNFRERSQDMEKFYIINRISKLLSLVMEGKEDDVSDLLMEPDSKLYEDIYSELLWSIHQELCEWYNFEEDTWSTMDRSQGVKPKPLYKDYSPIVEIFQGKAISKDLPQEVSFEKNKISLKGFSSIQEAFNSSLEVENKKITKEPLIIVLVVKEQGKVDLFNNESIRIGSYMFYLCSFITEEGLTSYSYAKNGDKDWYGYESNGVRNVLGMKDIRGCPVMLFYTRIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL50</accession>
    <name>I6ZL50_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AAA ATPase proteasome regulatory subunit YTA6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_110710</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265550.1">
        <property type="nucleotide sequence ID" value="XM_009267275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL50"/>
    <dbReference type="GeneID" id="20564670"/>
    <dbReference type="KEGG" id="ero:EROM_110710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110710"/>
    <dbReference type="HOGENOM" id="CLU_048761_0_0_1"/>
    <dbReference type="OrthoDB" id="39734at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF83">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="80"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="168"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="448" mass="52027" checksum="B33D5C57647D574A" modified="2012-10-03" version="1">MRDIEVSKSYRVEKKTKRDEEEYEEAQERTKGNMPEEKGTDVNNDILVALIYRKILKYYGIKQKKTKTPKTANPVRIKDVFYLAIVILIFGYVIYKVFIPNGGYEQPSKETKSEDRDKPSFRDGSSFENVSYFGKEDLMIQVISQVTKIVNLLRTVPEKDLHKSIETTQRNFLFHGPPGTGKTLFMKKLIYLVDLNIKFLKMRNEMGEKEFESMDHNEKLIRAKQMESLTRITFVTPSSLNNKYVGETEKNIRGLFQSANDGKYWATFVFIDEVDVFFSKRSDRSQDYTLKAQTEFLNTIGGACDRVNSTVFLFGATNLYDVLDDAFKRRFSGVYEFSLPNEEERLEILEEYLGDCNREVTNRLNDLVRMTNGMAQSRIVDILKKMSFTNDGSMDKIRWKDLRKAFEDADPKKKGTDGSSVEVVKSLKKFYFLEPRLKDKSVENTIGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS07</accession>
    <name>I6ZS07_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peripheral Golgi membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_010310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
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    </dbReference>
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    <dbReference type="GeneID" id="20520658"/>
    <dbReference type="KEGG" id="ero:EROM_010310"/>
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    <dbReference type="HOGENOM" id="CLU_502495_0_0_1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="2"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
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        <property type="entry name" value="GOLGI REASSEMBLY STACKING PROTEIN GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12893:SF0">
        <property type="entry name" value="GRASP65"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="74"/>
            <end position="156"/>
        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
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        <location>
            <begin position="439"/>
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        <location>
            <begin position="517"/>
            <end position="545"/>
        </location>
    </feature>
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        <location>
            <begin position="258"/>
            <end position="269"/>
        </location>
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        <location>
            <begin position="469"/>
            <end position="483"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="545" mass="61132" checksum="EBD348B13F8E4C85" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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    </dbReference>
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            <begin position="38"/>
            <end position="150"/>
        </location>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSD1</accession>
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        <name evidence="2" type="ordered locus">EROM_020410</name>
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    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
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        <lineage>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2192358at2759"/>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="192" mass="23023" checksum="86BFDF4A7A888756" modified="2012-10-03" version="1">MEISQFLEKILNKSTLSLEDIVFLLDKVDIMFKTPDCKILFYKNVEKALVHHFKETYEICIPSRVYSVNEIHGLILFLYIKKYGHPEVDLELERFFEGLFFGGIREMEVEQLKEETERLMRENEELRGRINEMNNAWIDRGFEEGEFKSVDEEVLSRMEKDLVALREQVDLEHPLILEAWYRLGEEYIKNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSV4</accession>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="838"/>
            <end position="863"/>
        </location>
    </feature>
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        <location>
            <begin position="838"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1238" mass="141596" checksum="5931BAAEFEBA87C2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSX5</accession>
    <name>I6ZSX5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_030700</name>
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            <taxon>Fungi</taxon>
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        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264188.1">
        <property type="nucleotide sequence ID" value="XM_009265913.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSX5"/>
    <dbReference type="GeneID" id="20520981"/>
    <dbReference type="KEGG" id="ero:EROM_030700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030700"/>
    <dbReference type="HOGENOM" id="CLU_015718_1_1_1"/>
    <dbReference type="OrthoDB" id="1662883at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097435">
        <property type="term" value="P:supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000009">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013838">
        <property type="entry name" value="Beta-tubulin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="12">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="13">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="439" mass="49115" checksum="0F4B1A994659566D" modified="2012-10-03" version="1">MREIIHLQTGQCGNQVGCKFWETISGEHGIDQTGRYVGTSDNQLERVNIYYNEASSKKYVPRAVLIDLEPGTMDAVRQGPFGDLFRPDNFVFGQSGAGNNWAKGHYTEGAELIDSVMDVVRKEAESSDCLQGFQITHSLGGGTGAGMGTLLLSKIREDFPDRMICTFSVVPSPKVSDTVVEPYNATLSIHQLVENADETFCIDNEALYDICFRTLKMSNPGYGDLNHLVSLVMSGVTTCLRFPGQLNADLRKLAVNMIPFPRLHFFVVGFAPLIAIGTQKFKTYSVSELTQQMFDSKNMMTACDPRKGRYLTVTAIFRGKISMKDVDEQMSMVQSKNSSLFVEWIPSNVKTAVCDIAPTGLEMSATFVGNTTSIQELFKRISDQFTVMFRRKAFLHWYTGEGMDEMEFSEAESNMNDLLSEYQQYQDATVEDTEEFLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT07</accession>
    <name>I6ZT07_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(d)CMP kinase</fullName>
            <ecNumber evidence="2">2.7.4.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_031140</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="2"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264233.1">
        <property type="nucleotide sequence ID" value="XM_009265958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT07"/>
    <dbReference type="GeneID" id="20521027"/>
    <dbReference type="KEGG" id="ero:EROM_031140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031140"/>
    <dbReference type="HOGENOM" id="CLU_079959_0_2_1"/>
    <dbReference type="OrthoDB" id="10263145at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidylate kinase" evidence="9">
        <location>
            <begin position="6"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="220" mass="24994" checksum="6BCAF9D134D13DE3" modified="2012-10-03" version="1">MKVYKIAVDGPAASGKSSASDLVAKKLGFSHLISGNLYRAVTYALVSQFEEVQPKDEEQKRFVLGLDMEIRNNRVFLNGKDISDYLRGEKIDYHVTSIAKEKYVREKVSVIQKSTIKLEKKGIIVDGRDIATKIMPEADLKIFLTASPETRAKRRYKESKSESYEELLEAMIKRDRGDRTREHGPLVAAEGSIIIENDCMTLEETADEIVRLFKRVESFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT13</accession>
    <name>I6ZT13_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Histone deacetylase</fullName>
            <ecNumber evidence="15">3.5.1.98</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ordered locus">EROM_031240</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>N(6)-acetyl-L-lysyl-[histone] + H2O = L-lysyl-[histone] + acetate</text>
            <dbReference type="Rhea" id="RHEA:58196"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:11338"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="3.5.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the histone deacetylase family. HD Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.98" evidence="15"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264243.1">
        <property type="nucleotide sequence ID" value="XM_009265968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT13"/>
    <dbReference type="GeneID" id="20521038"/>
    <dbReference type="KEGG" id="ero:EROM_031240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031240"/>
    <dbReference type="HOGENOM" id="CLU_007727_7_4_1"/>
    <dbReference type="OrthoDB" id="1918432at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032221">
        <property type="term" value="C:Rpd3S complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141221">
        <property type="term" value="F:histone deacetylase activity, hydrolytic mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09991">
        <property type="entry name" value="HDAC_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.800.20:FF:000001">
        <property type="entry name" value="Histone deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003084">
        <property type="entry name" value="HDAC_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF14">
        <property type="entry name" value="HISTONE DEACETYLASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037913">
        <property type="entry name" value="His_deacetylse_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01271">
        <property type="entry name" value="HISDACETLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0678">Repressor</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="11 15" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="20">
        <location>
            <begin position="23"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="373"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="393"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR037913-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="AFN82746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="415" mass="46665" checksum="D21A3DDD3BD46AEB" modified="2012-10-03" version="1">MNNKRVVYFYDPEIGTFSYAMGHPMKPLRVKMTHTLLVGYELYKYMDVLRPFQASYENLTNFHAVDYINFLSSVSSENMEEMIKDLHKFNIRDDCPVFPGLYDYCKLTSGGSIYAAQKINSGKYDVAINWAGGLHHAKRSEASGFCYVNDIVLGILELLKYNKRVLYIDIDVHHGDGVEEAFYTTDRVMTVSFHKYGDYFPGTGMVTDIGLAKGKGYSVNVPLRDGIDDESYFSIFKPVVSKVIEVYRPDAIVLQSGADSLSGDKLGCFNLSHLGHSRCIKFVQSFNIPLILLGGGGYTIGNVSKAWAYGTSVVLDVEIPREIPYNEYFDYYAPTYKIDVPTSNMKNQNTKESLEDIVAKVHENLRGVSHAPSVQMSTIPPSFISDESDDEEISKKKIGEDSDDYKAFSGSREEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT17</accession>
    <name>I6ZT17_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Vesicular-fusion protein SEC18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_031290</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264248.1">
        <property type="nucleotide sequence ID" value="XM_009265973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT17"/>
    <dbReference type="GeneID" id="20521043"/>
    <dbReference type="KEGG" id="ero:EROM_031290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031290"/>
    <dbReference type="HOGENOM" id="CLU_008037_2_0_1"/>
    <dbReference type="OrthoDB" id="9982946at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035494">
        <property type="term" value="P:SNARE complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000115">
        <property type="entry name" value="N-ethylmaleimide-sensitive fusion protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000154">
        <property type="entry name" value="Vesicle-fusing ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000166">
        <property type="entry name" value="vesicle-fusing ATPase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039812">
        <property type="entry name" value="Vesicle-fus_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078:SF3">
        <property type="entry name" value="VESICLE-FUSING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23078">
        <property type="entry name" value="VESICULAR-FUSION PROTEIN NSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="223"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="504"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="678" mass="77475" checksum="9919DACF301F52DC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT44</accession>
    <name>I6ZT44_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_040220</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264288.1">
        <property type="nucleotide sequence ID" value="XM_009266013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT44"/>
    <dbReference type="GeneID" id="20521086"/>
    <dbReference type="KEGG" id="ero:EROM_040220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040220"/>
    <dbReference type="HOGENOM" id="CLU_027721_3_0_1"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="110"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="306" mass="35901" checksum="86CDD428391BEF2F" modified="2012-10-03" version="1">MGWKGFWSEAFRVAIFIIYPTLELYIYFVFVGLLCLEDTEFNSWTIFALFVAYHVIVTYKAMFYMKLFINEGVSTLEIFPDVPQEGYDLKMKGMNKFVEEEVMKQTVAKIKLCSKCKTYKPPRAHHCGTCKRCYLKYDHHCALLNTCIGFHNYKFFYQFMVLNLVSVIFFIVTISIYMMLHTPKSTTHYVNYIVSISLLGVEFIFNLSLLIFHTWLIGMNETTIEHYALNDYINGDHSFSHIFQEGPMTTLTDSTDRRTLNPYNLGLKRNWKQVFGNSFMDWVTPSYSTLGNGITFAKNYDEYELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTB6</accession>
    <name>I6ZTB6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pyruvate dehydrogenase E1 component subunit beta</fullName>
            <ecNumber evidence="5">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_041150</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + pyruvate + H(+) = N(6)-[(R)-S(8)-acetyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:10478"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="5"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264378.1">
        <property type="nucleotide sequence ID" value="XM_009266103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTB6"/>
    <dbReference type="GeneID" id="20521178"/>
    <dbReference type="KEGG" id="ero:EROM_041150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041150"/>
    <dbReference type="HOGENOM" id="CLU_012907_1_1_1"/>
    <dbReference type="OrthoDB" id="10266385at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006086">
        <property type="term" value="P:pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07036">
        <property type="entry name" value="TPP_PYR_E1-PDHc-beta_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000001">
        <property type="entry name" value="Pyruvate dehydrogenase E1 beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027110">
        <property type="entry name" value="PDHB_mito-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006667">
        <property type="entry name" value="PRK09212.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624">
        <property type="entry name" value="DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11624:SF96">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5 7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="6">
        <location>
            <begin position="2"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="327" mass="36194" checksum="3B483DC7BA360738" modified="2012-10-03" version="1">MITVREALNQAIDEEMERDERVFVLGEEVGVSGGNNEVTKGLYEKYGKWRVLDTPISEMGFTGLAVGASYLGLRPIVEFMTWNFALQSIDHIINSCAKTLYMSGGKISCPVVFRGPNGFNPGYAAQHTQDFCNYYGAVPGLKVVAPCTARDHKGLLKCAIRDDNPVVFLENETLYNDIYDDIEEGYIQPLDRAVIEVEGNDITLIGISLSVKVCMEAAEALKDVGISCEVINLVSIRPIDKKTLLSSAKKTKHVFVVDFSWPSFSVASEVSAIIHEQCFGDLAVPVQRINGEDVPTPYSEEVERMAFPTYLDVVSSVINIYKNGRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTE0</accession>
    <name>I6ZTE0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264413.1">
        <property type="nucleotide sequence ID" value="XM_009266138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTE0"/>
    <dbReference type="GeneID" id="20521215"/>
    <dbReference type="KEGG" id="ero:EROM_041520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041520"/>
    <dbReference type="HOGENOM" id="CLU_573756_0_0_1"/>
    <dbReference type="OrthoDB" id="14421at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="512" mass="57699" checksum="2E7F49F0E7E2263F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTF7</accession>
    <name>I6ZTF7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264438.1">
        <property type="nucleotide sequence ID" value="XM_009266163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTF7"/>
    <dbReference type="GeneID" id="20521240"/>
    <dbReference type="KEGG" id="ero:EROM_050070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050070"/>
    <dbReference type="HOGENOM" id="CLU_1713232_0_0_1"/>
    <dbReference type="OrthoDB" id="2193909at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031527">
        <property type="entry name" value="DUF5091"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17012">
        <property type="entry name" value="DUF5091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="153" mass="17828" checksum="688A164CB24BCC9D" modified="2012-10-03" version="1">MEGFIHKINNLLKNPRLEDILSVFEIDGYIRLNRVYISPFSLRQLLIRLAGLDLVSWQMTPSGLFFQFRAFCLKFYGSVNIVVKQKVFCIEIDFDPSIDGGAGVPVLRELERYLKGCDALFFMTYYTDIDKEILHNPLSESLDFLYRKSSIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTM2</accession>
    <name>I6ZTM2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264513.1">
        <property type="nucleotide sequence ID" value="XM_009266238.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTM2"/>
    <dbReference type="GeneID" id="20521316"/>
    <dbReference type="KEGG" id="ero:EROM_050840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050840"/>
    <dbReference type="HOGENOM" id="CLU_1421401_0_0_1"/>
    <dbReference type="OrthoDB" id="2191234at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="181" mass="21477" checksum="7B07F30FA1FFF339" modified="2012-10-03" version="1">MFEYYEEPIFTNDLLSLMYILDGNVDRIVFTSRMDPKVFPKEDTVPTKYAEVLSSFGSFLDFYRDLKEEGEHAEIIAYYKLKHSRLSFMALHSKYVQIIKKMDKEEDITYYNKNLYKTLLECNEYASNRGSSDMFLAPFYTSRFFKQNGELGKSRRLRKEARRIARISNRLNAIQEMDNGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUC7</accession>
    <name>I6ZUC7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264768.1">
        <property type="nucleotide sequence ID" value="XM_009266493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUC7"/>
    <dbReference type="GeneID" id="20521579"/>
    <dbReference type="KEGG" id="ero:EROM_070200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070200"/>
    <dbReference type="HOGENOM" id="CLU_933924_0_0_1"/>
    <dbReference type="OrthoDB" id="25179at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="1">
        <location>
            <begin position="81"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="297" mass="35022" checksum="04DEB38B00F4D0A8" modified="2012-10-03" version="1">MSSKELTTIIKEYSSSGTSESSCGDQLVVREFSNAYDEETDMGVVWNGDILPHSWYQEQIFNRRKAKRYEYKIKKAEAKDIYHMTPRKLSKALTFIDLSILQRITPNELVDYDGPGNTKCRNIRHMRSKNVGLTNFVSNELLREQNYKYFFKLLKHLERIGNYNSFYCIIKAFQMQKLDLKRLNTLSRHMEKSTSYFDMRQFLDDLTTANTFLICPMDVYIKDVEESNRTRNNEVASMRFCRLVEILIKLQSQTLDASISHADEHFLFSKFWHYFQRGSSSSRKPGTKKYDGQFLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUJ6</accession>
    <name>I6ZUJ6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264863.1">
        <property type="nucleotide sequence ID" value="XM_009266588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUJ6"/>
    <dbReference type="GeneID" id="20521675"/>
    <dbReference type="KEGG" id="ero:EROM_071150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071150"/>
    <dbReference type="HOGENOM" id="CLU_170488_0_0_1"/>
    <dbReference type="OrthoDB" id="10433024at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="113" mass="13002" checksum="5EE5628CE417A191" modified="2012-10-03" version="1">MRNQEEEKIEEKKEADVGPAKSGVIDGQKTSSARDPEPKQSVSSQEREENPKSYCINEFKEYITEIPFPIKEKTGIMKQFLSGVEKLFAIVKSSIRKAILWISRKNLRDEEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUM7</accession>
    <name>I6ZUM7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal-protein-alanine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_071600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264908.1">
        <property type="nucleotide sequence ID" value="XM_009266633.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUM7"/>
    <dbReference type="GeneID" id="20521724"/>
    <dbReference type="KEGG" id="ero:EROM_071600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071600"/>
    <dbReference type="HOGENOM" id="CLU_013985_7_1_1"/>
    <dbReference type="OrthoDB" id="10264728at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031416">
        <property type="term" value="C:NatB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051646">
        <property type="entry name" value="NatB_acetyltransferase_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45910:SF1">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="181" mass="20895" checksum="57BD746DE1409983" modified="2012-10-03" version="1">MYKVEPMLPSDIFSLDWVNLDEKSESFPLSYYLYYLVNHGEDCIVIPSLPEYKTSFTYKRNVCAYMIGKLEEKDGFINAHISAVSVAPSYRKNRFGRLCMSVIEKSGDVYNAHFADLYVREGNITAIEFYKKLGYAVYRKVFNYYVDGDGENALDMRKSLKMDRNKVCMARGADIQASELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUQ2</accession>
    <name>I6ZUQ2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIF4G-like type 2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264938.1">
        <property type="nucleotide sequence ID" value="XM_009266663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUQ2"/>
    <dbReference type="GeneID" id="20521755"/>
    <dbReference type="KEGG" id="ero:EROM_080190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080190"/>
    <dbReference type="HOGENOM" id="CLU_702136_0_0_1"/>
    <dbReference type="OrthoDB" id="2188448at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G-like type 2" evidence="1">
        <location>
            <begin position="307"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="393" mass="45208" checksum="40715BBD587A26C9" modified="2012-10-03" version="1">MQLPIARINSTNYRRILEEVRLSEDKERAIEDIKNVVLLMPHKSSIAANLISDLIKDDGDFRSGIVKMVDEILACGDGCMLISASFTLKKLGVGGMESLWWTKEIPTVSPLLESVNFKVPPDSLNECKEEAEKVLRITDEKNFEEVFCVSQAIRNFRFSIQECVTQLGYISRLKSLVDGIRVLQQKENGLYLSALVLELARKQGFMRVLLEELSSFDREFKDILIPLLFEHFYNPSEENNSVYVSSSYMPLRTSEDINLFKQLFTEDIVRNMKRISGSSKVEKFLDKGKDEGTKKVPRISREEFEKTDLKDREAFFKNFCFLGSPSISHFLTYLEIYKERFVLNKEDQKLFISIFFEIFEGLESFCRIVTEKMVRFGIIDPALTANPGTNPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV99</accession>
    <name>I6ZV99_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nuclear pre-ribosomes export protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265148.1">
        <property type="nucleotide sequence ID" value="XM_009266873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV99"/>
    <dbReference type="GeneID" id="20521972"/>
    <dbReference type="KEGG" id="ero:EROM_090340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090340"/>
    <dbReference type="HOGENOM" id="CLU_611398_0_0_1"/>
    <dbReference type="OrthoDB" id="10263597at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011501">
        <property type="entry name" value="Noc3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016903">
        <property type="entry name" value="Nucleolar_cplx-assoc_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14428:SF5">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07540">
        <property type="entry name" value="NOC3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar complex-associated protein 3 N-terminal" evidence="1">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="450" mass="53037" checksum="60D2D5182D2F52BA" modified="2012-10-03" version="1">MNKMDSQKSRIASICTKIIEEPQENLKMMEELFRMLSDGRSEGGAKGIIYLSLLKVFKAIIPLYKVRSLKDIVKDKRDNLHIKDYDRSLLKWYASYIKILVDDMSDESYISLCEVLQHFDHFNCTDKIVSGVLRGSLGRRPVSMLCCDTIKSKLRSDCSGELIVIILDQMLDFEHNPLVLEYLVDIPFVNNSLKSDEEKAREYWEANRSVSRREKNSIFHRKQIFSKKLKKMEKERLKIQSQVRDEEDIEERVEEIKTCKKIYDVIQRLYFTILKRNRYEYYKYVFIGLVKYRKILRPEFMEGLYFLLNNNLSKVPPDAKIQGILCILTLYGDECYDFNGLVDLVYSMIHPMNPELADNDGVSKAIKLLFIKIRQPIHRAHVLLKRLILCCCSRSIPEFRLLIKDISAYYDIEFSDCTSPKCREFDQDAVHVDSIPDNPFFEYFLYKQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVE9</accession>
    <name>I6ZVE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265203.1">
        <property type="nucleotide sequence ID" value="XM_009266928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVE9"/>
    <dbReference type="GeneID" id="20564314"/>
    <dbReference type="KEGG" id="ero:EROM_090900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090900"/>
    <dbReference type="HOGENOM" id="CLU_029501_2_1_1"/>
    <dbReference type="OrthoDB" id="271595at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="3">
        <location>
            <begin position="49"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="210" mass="24541" checksum="1E65920B4C794E1F" modified="2012-10-03" version="1">MEDPRRFEERFVHKFYNENTREFSATRRKHWKMTEKFLDSYYTSGSIVLDAGCGNGRSFLVPEVVGLDYCFDLLRDAKAIRNHGLVRGDVLKLPFADSSFDLVLSVGVIHHLSTYERRLKAMEEMKRVLKDGGKGLVYVWGNSVKDKKKFSKIIGRPGEEYFATWNLRDDTKRYYYLYDIEGLLELCRDSGFKVLNYGPEEESLFTVLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVX5</accession>
    <name>I6ZVX5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S acidic ribosomal protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265408.1">
        <property type="nucleotide sequence ID" value="XM_009267133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVX5"/>
    <dbReference type="GeneID" id="20564525"/>
    <dbReference type="KEGG" id="ero:EROM_100950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100950"/>
    <dbReference type="HOGENOM" id="CLU_175004_0_0_1"/>
    <dbReference type="OrthoDB" id="2193863at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="101" mass="11374" checksum="C8983125296C4C78" modified="2012-10-03" version="1">MTQEKMINDMYPLATLLIHATNQQVTKEKIDSVFKFFGLASHPKICEFFEMDALEIKNLLMSSTQEAAAPMEGKGPSDTPGEAKKEEVPEEEEVEIDFGMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW39</accession>
    <name>I6ZW39_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">M7GpppX diphosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HIT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265473.1">
        <property type="nucleotide sequence ID" value="XM_009267198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW39"/>
    <dbReference type="GeneID" id="20564591"/>
    <dbReference type="KEGG" id="ero:EROM_101610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101610"/>
    <dbReference type="HOGENOM" id="CLU_041045_1_0_1"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.40">
        <property type="entry name" value="Scavenger mRNA decapping enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008594">
        <property type="entry name" value="DcpS/DCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011145">
        <property type="entry name" value="Scavenger_mRNA_decap_enz_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978">
        <property type="entry name" value="HISTIDINE TRIAD HIT PROTEIN MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12978:SF0">
        <property type="entry name" value="M7GPPPX DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05652">
        <property type="entry name" value="DcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11969">
        <property type="entry name" value="DcpS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102860">
        <property type="entry name" value="mRNA decapping enzyme DcpS N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="280" mass="33224" checksum="0B04DED6F61A3C38" modified="2012-10-03" version="1">MFSIRDLSIICKNLDPNMKDIIKEFILDQSLVNSEGNLYIGRIRDKKAILIFPRQSVSKDTFIQFLSLPMENIQSNNIYYSYRVLGPIYINFRVIYPATEDHIKKYSPRLVYVKETYKEYLDFINNHAHISTNWMDNITLKNKRNVNENILYEDGEIMIIPDYKWNCQNIDALHLLIIFKDLGLKTVRDIDNWEILVRAQEKASNVLETMFSLDFNHVFMFFHYRPTYLRLHIHVINIKIAYEGTASSIRAIPLHDVIHNLRTDPSYYKKDMFVLQSEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW89</accession>
    <name>I6ZW89_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265528.1">
        <property type="nucleotide sequence ID" value="XM_009267253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW89"/>
    <dbReference type="GeneID" id="20564648"/>
    <dbReference type="KEGG" id="ero:EROM_110490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110490"/>
    <dbReference type="HOGENOM" id="CLU_063261_0_0_1"/>
    <dbReference type="OrthoDB" id="2186004at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="289" mass="32689" checksum="0A5B53B76EB9D602" modified="2012-10-03" version="1">MADTDSEKKSQCRQLCPIKIPLMSPLRDMIVREVGDKASTVIDVIEGIVPTVSPESMYSELVEALIPVIDQRAKGLVGKVMAYKRKTCRDGDECRTKNCIFLHGWDRRGGEGASGNSGLKGKEEGVDIVETAPKRRKITESNENNEVVFNKVNESRHNPEDLKTYAARFGRITNFKRLNPEKYLVVFEDPESANELVKSSELVLEDPGIKKFYNVIDNLVKVELRKLFEEQEIIIDKMSTDFSAALLGHLRNINIRIRALVLKDKSKDGSGVDNKRGFDQENSLYYNCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWJ3</accession>
    <name>I6ZWJ3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA pseudouridine synthase</fullName>
            <ecNumber evidence="2">5.4.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111500</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="2"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265628.1">
        <property type="nucleotide sequence ID" value="XM_009267353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWJ3"/>
    <dbReference type="GeneID" id="20564749"/>
    <dbReference type="KEGG" id="ero:EROM_111500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111500"/>
    <dbReference type="HOGENOM" id="CLU_014673_4_0_1"/>
    <dbReference type="OrthoDB" id="25767at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF5">
        <property type="entry name" value="TRNA PSEUDOURIDINE(38_39) SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="3">
        <location>
            <begin position="183"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="312" mass="36179" checksum="558A51FBACF81E30" modified="2012-10-03" version="1">MKSNICERLQNPGNEGNVKKMHQEKHKKALKCLSQRTVALKISYNGEGYSGVSPQNGRKTIGGNLEYALKATGLGEKLVYAGRTDSGVSAINMVASGVVISRVLEPNRSYVITKDDHKEYKYDIMLNNYLPSDIRVIGWAPVPDTFNARFTCIQRQYRYYFHKEGLDINEMNQAASRIKDINNFYYFSKHSDKNARYERKLDECRIIDDGDIYYLDIKARAFLHNMVRKIVWAVQKSGKGEEYDIQRIGTSEPYPLVFCGAVYPYELSFINNLRCCSELRYRMERDQINYKISRDRLEHLANELSKEVRSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWK7</accession>
    <name>I6ZWK7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Farnesyl pyrophosphate synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111700</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265648.1">
        <property type="nucleotide sequence ID" value="XM_009267373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWK7"/>
    <dbReference type="GeneID" id="20564769"/>
    <dbReference type="KEGG" id="ero:EROM_111700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111700"/>
    <dbReference type="HOGENOM" id="CLU_028376_3_0_1"/>
    <dbReference type="OrthoDB" id="10257492at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:(2E,6E)-farnesyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045337">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039702">
        <property type="entry name" value="FPS1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525:SF0">
        <property type="entry name" value="FARNESYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11525">
        <property type="entry name" value="FARNESYL-PYROPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="292" mass="33629" checksum="8FB413C03E62403D" modified="2012-10-03" version="1">MIKETVKDKIMEMSGDYNRSEVSFLLDYNLDGGKELRYKAYIHVLKELGGDLNERNLIMGYSVELLQAALLIIDDLMDNSELRRGKPCYYFKRGAKTLKDAFFLLAIIRNLLDEKAKNAYSSSIFKTCLGQTHDTIRKTRSEYSVKTYTAIAESKTGAYTFYLPAILGYVSAGKEEPKRLWNFCNLGALIFQMQDDYLNFFPEKSGKSMNDLEEMKCTWFTSRISHMKDPAVERYFSEGLVTPDLLSIIRGLFREYSSNLDELLSRLLSTVDEDEKAVLSVFISFLESRKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWM6</accession>
    <name>I6ZWM6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Exoribonuclease phosphorolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_111950</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265673.1">
        <property type="nucleotide sequence ID" value="XM_009267398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWM6"/>
    <dbReference type="GeneID" id="20564794"/>
    <dbReference type="KEGG" id="ero:EROM_111950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111950"/>
    <dbReference type="HOGENOM" id="CLU_1200154_0_0_1"/>
    <dbReference type="OrthoDB" id="10264038at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF9">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="9">
        <location>
            <begin position="160"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="226" mass="25259" checksum="2E2FFF804DC6CEC4" modified="2012-10-03" version="1">MEGFSQDELEFVRNGIAKGHRSDLRKSKEERKAHVICSSIAQGDGSVRVKRGWSEIEISIQFKETPETLGASNVPGEILGSNELYMESIEFSKIAIPKIITSKILELLSSYKVGIRVEFSILSNDGNIYDLFFIGLSTLFKELEVPVIEDLRKTVKREIDIPVSKTVALFNNGYFVVDPTMIEEEASCGLMHVFISNEGKLMGCLSEGNCDIDQEVFVNIIKEISI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACS5</accession>
    <name>I7ACS5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Elongator complex protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_010400</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the WD repeat ELP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263882.1">
        <property type="nucleotide sequence ID" value="XM_009265607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACS5"/>
    <dbReference type="GeneID" id="20520667"/>
    <dbReference type="KEGG" id="ero:EROM_010400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010400"/>
    <dbReference type="HOGENOM" id="CLU_427051_0_0_1"/>
    <dbReference type="OrthoDB" id="27911at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037289">
        <property type="entry name" value="Elp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44111:SF1">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="159"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="207"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="485"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="528"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="613"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="646" mass="73227" checksum="2790F0C43A70C769" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD09</accession>
    <name>I7AD09_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">EROM_020100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263982.1">
        <property type="nucleotide sequence ID" value="XM_009265707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD09"/>
    <dbReference type="GeneID" id="20520768"/>
    <dbReference type="KEGG" id="ero:EROM_020100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020100"/>
    <dbReference type="HOGENOM" id="CLU_2223260_0_0_1"/>
    <dbReference type="OrthoDB" id="10595786at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="106" mass="11890" checksum="37C8A226A6C9D2AE" modified="2012-10-03" version="1">MKKGLLALCAFAISDAKSLHFRHEKQRNELHKASGLGASNLVSKSMSKGSFLLPVSNVRGKKKRREKKRKGSSESESESENESESSEENKKTRKKKKKGKKSKNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADI0</accession>
    <name>I7ADI0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264122.1">
        <property type="nucleotide sequence ID" value="XM_009265847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADI0"/>
    <dbReference type="GeneID" id="20520913"/>
    <dbReference type="KEGG" id="ero:EROM_030060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030060"/>
    <dbReference type="HOGENOM" id="CLU_085830_0_0_1"/>
    <dbReference type="OrthoDB" id="2191229at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="285" mass="33560" checksum="78F207865ABA0BDF" modified="2012-10-03" version="1">MNRYETADHDYMHAKDYFNNYKFGYIERTTKTLLLQSLRPEDRQNEDLLTILNQSKSQLKDVKSIGKEASKSISELSELIYEARNKLSRYEEMLKYETEREKSLKEECTRFESLDENLKVYDELTAQFDRGCKEMQVNIEKINVLKEEIAMMSTNEAEEELKSIKNRRDKLCGKQKRPSLITMESYIENSYNWYRKALEFIKNVFGISLVTIEQENNEMYMRLKASTCEIGIFVRDGRMIESKLYNTSDESLMSLFPALNKFAVSINDPRTLLMLVANRCTTSED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADL7</accession>
    <name>I7ADL7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1764 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264152.1">
        <property type="nucleotide sequence ID" value="XM_009265877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADL7"/>
    <dbReference type="GeneID" id="20520945"/>
    <dbReference type="KEGG" id="ero:EROM_030340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030340"/>
    <dbReference type="HOGENOM" id="CLU_179459_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013885">
        <property type="entry name" value="DUF1764_euk"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08576">
        <property type="entry name" value="DUF1764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="11334" checksum="D93C181CF3B4EF16" modified="2012-10-03" version="1">MRKTKKNNTRSKNSPRDGLEGGKGHKTENDIEAIFSKVDSPKICKVKEGKEDEESDKGRYFSVGEGGRRYHEGLPVYTLEELQLGDGGGDTEDCPIYCDCCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADT0</accession>
    <name>I7ADT0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264237.1">
        <property type="nucleotide sequence ID" value="XM_009265962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADT0"/>
    <dbReference type="GeneID" id="20521032"/>
    <dbReference type="KEGG" id="ero:EROM_031180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031180"/>
    <dbReference type="HOGENOM" id="CLU_748082_0_0_1"/>
    <dbReference type="OrthoDB" id="2195834at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="370" mass="43585" checksum="F46D813E99A47C03" modified="2012-10-03" version="1">MDSKIEFSEVDDEDVQSEVNDKLGSRTFWVLFWISMTCILIVILRLLETYFVYDVFSRTIPGSWYLLWLPKKTLYYLGAEKFLEYKYEEYMEGMPKELEMLLSVKRNFCMTFGLLILVNIASELFPISLIHALNLKFKAIDFWRPVMIGIRICILTTLLRGKNIKEITDGLSPESVNNLLAAQYCFTSAWELFKEIENNTVIEVIVRVIGYWIITTTISKETSSLIESSVLERIIRHTNIYFSKKHPETTPNSDIQKKFLDFLKSELSSKSMSYLLATILTPFFCIFMTYFLLKTTEKFEQMVYRELEISDKFIRPYIRVLTIIFIILFLLYAYHEILTVVDRLEEKVRTEKMFSNPNQKELCDLISSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE69</accession>
    <name>I7AE69_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264427.1">
        <property type="nucleotide sequence ID" value="XM_009266152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE69"/>
    <dbReference type="GeneID" id="20521229"/>
    <dbReference type="KEGG" id="ero:EROM_041655"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041655"/>
    <dbReference type="HOGENOM" id="CLU_2868255_0_0_1"/>
    <dbReference type="OrthoDB" id="2188726at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7844" checksum="D80E2C7F829F3355" modified="2012-10-03" version="1">MPANINCKKKWHPSRYETQIRVREAEKAIEKSKIQEDTRRELGRREVLESEIHGEYTERMQWML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEE9</accession>
    <name>I7AEE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264537.1">
        <property type="nucleotide sequence ID" value="XM_009266262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEE9"/>
    <dbReference type="GeneID" id="20521341"/>
    <dbReference type="KEGG" id="ero:EROM_051090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051090"/>
    <dbReference type="HOGENOM" id="CLU_176428_0_0_1"/>
    <dbReference type="OrthoDB" id="1746530at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030880">
        <property type="term" value="C:RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="107" mass="12391" checksum="E6741214264E898C" modified="2012-10-03" version="1">MKINSKFRLMTKIETLDAINRSLETHPGQAEFLEKVRLYCTSSMAKDQALGIKQTLINIGLTEFEIIQLLDFNPKSIVCLQLVIEDMEERFTEEALLRILDLFNDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEG6</accession>
    <name>I7AEG6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin carboxyl-terminal hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264557.1">
        <property type="nucleotide sequence ID" value="XM_009266282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEG6"/>
    <dbReference type="GeneID" id="20521361"/>
    <dbReference type="KEGG" id="ero:EROM_051300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051300"/>
    <dbReference type="HOGENOM" id="CLU_008279_1_1_1"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF937">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="129"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="413" mass="47132" checksum="AB82873C7692F60F" modified="2012-10-03" version="1">MSSSDMIEGYTHIMDLCAHFEALKDVEKTFKMIVSKLDCLKKRGVRPRCQKCKATLSLRVCMQCYCFLCSIDERLHEHTMFLDLFYDLISCISCTKRYLPSNIVNRSGSTVLVKVPGPLYEILPYVPIKGFSNLGNTCYINAILSIMVNLSPIRDELLLSTRIRARCSGSRCIICAMKSLIDSSYGSGCVLTHKFIHTFWLLAPHLASPRQQDAHEFFICLLQKIHEAYSPSTIDDLVCRCLSHRIFFGTFSSTTNCRRCNLGRAKEEAFATISLECLPSIQSSLDIFFEPEDLKCEMACNTCKTKTLCTRRLEVKKHPRILSLHMKRFSYDKVPRKIDSHVALSSTLTIGKRVYDILGFVSHYGSINCGHYLSYIILGGKWHKIDDEKVDIVPHRELSTDGLYTAYYLRRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF18</accession>
    <name>I7AF18_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM Nup35-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_070140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264762.1">
        <property type="nucleotide sequence ID" value="XM_009266487.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF18"/>
    <dbReference type="GeneID" id="20521572"/>
    <dbReference type="KEGG" id="ero:EROM_070140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070140"/>
    <dbReference type="HOGENOM" id="CLU_128908_0_0_1"/>
    <dbReference type="OrthoDB" id="1733656at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12441">
        <property type="entry name" value="RRM_Nup53_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007846">
        <property type="entry name" value="RRM_NUP35_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05172">
        <property type="entry name" value="RRM_Nup35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51472">
        <property type="entry name" value="RRM_NUP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4 5" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RRM Nup35-type" evidence="6">
        <location>
            <begin position="52"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="150" mass="17322" checksum="20069F745356561E" modified="2012-10-03" version="1">MRFPSLLMTEFHPSLFRRKNKSEDTPEVVPETKSTSIPQNTLYEEPLVSQHRVKKKYVTIYGFTPRNLDAVIDAVKSCGDVLEVEYGKNWINVLFDNEESIKRCLRLNTTMIEDEIIGAFRQGGGIIEDHDIFVRKKGVFTSIMEYLFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF80</accession>
    <name>I7AF80_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264842.1">
        <property type="nucleotide sequence ID" value="XM_009266567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF80"/>
    <dbReference type="GeneID" id="20521654"/>
    <dbReference type="KEGG" id="ero:EROM_070940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070940"/>
    <dbReference type="HOGENOM" id="CLU_669074_0_0_1"/>
    <dbReference type="OrthoDB" id="2194840at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="beta-trefoil_Ricin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="399" mass="44961" checksum="02365B3FE87DD5F6" modified="2012-10-03" version="1">MRVRSGIYLFLRIAASKDFLATIRPINSDKVFRTVGESIELSPNRGEDLAIFHVVTKKEKRFKLFGMRLGKHRHMLLISPGSNPELYLGYSKEKGLGMQKKRKYWEYREVGNGGYILKAKKNKCLGVAGNNTLGVFKCRNEEGQVFIFEKKNSHETSGSTSEDSHIAITSEPPQVPGTPYVQDKSWEDFQTSQESSDSSSDSSIVRPVFINLNMKDKKAYADDQEVASLSVMTSTTTVSVTITRSSSIREVSTTHPMSVVRNSIVITTRIPSSVASIWNKMAATEELQTSYDSSSSSEVHRMPFNKASNGHYEKIKDGMNLDLDFEFYPELGRLLKSDGYRRSNPHLKKREARKKNKAKYRDKMKENRILSSGLECFDSCVLNPLQQTGVKAPPSANIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFG2</accession>
    <name>I7AFG2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264947.1">
        <property type="nucleotide sequence ID" value="XM_009266672.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFG2"/>
    <dbReference type="GeneID" id="20521764"/>
    <dbReference type="KEGG" id="ero:EROM_080280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080280"/>
    <dbReference type="HOGENOM" id="CLU_063929_0_0_1"/>
    <dbReference type="OrthoDB" id="2190712at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="366" mass="42315" checksum="18FE09738CFBC526" modified="2012-10-03" version="1">MSKEAVDDGDESGFSYEYTSSDSLETISEEIEEYEREYFGDVSEEGSSRSEDVAAWEDSDDDEKIRENYKKTATWLDGKQTKKQVEVRKKRMRFEYEKHLFFSKCRIKLAKARGEYLVVVDTYSNIYILKNFKVHKTLWIEMFGVSDFVYIGDVILFSSCRQSNLKEITFDGEVRNISIRTIEGIRKMASIGDSIYVAGERLVLLNNAYEVVETIEGRFMDIAVSEEYVYAMSFSGEIIILTRDLQVLKKVSLEDKFDFRSIHFFADRIFIGMGMGIKIFDRDMKPVKEIMNLKNEVTGITRHGDYVVYGSNYTNSLKMILPDLKCYNKFPFNTINISPIKALESDGKNIIICSGRAINVLRIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFM5</accession>
    <name>I7AFM5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265037.1">
        <property type="nucleotide sequence ID" value="XM_009266762.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFM5"/>
    <dbReference type="GeneID" id="20521859"/>
    <dbReference type="KEGG" id="ero:EROM_081240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081240"/>
    <dbReference type="HOGENOM" id="CLU_2542574_0_0_1"/>
    <dbReference type="OrthoDB" id="2191106at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="83" mass="9322" checksum="491A3566E4400B4C" modified="2012-10-03" version="1">MYTRYRLVFARKGARVEDGVLQEFVQEIIRGFGGLDLLSRVDVKCNGGIIGVEADEEASNVVHGSLFFCGEYKSVKCRFEQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFN7</accession>
    <name>I7AFN7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265052.1">
        <property type="nucleotide sequence ID" value="XM_009266777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFN7"/>
    <dbReference type="GeneID" id="20521874"/>
    <dbReference type="KEGG" id="ero:EROM_081390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081390"/>
    <dbReference type="HOGENOM" id="CLU_1938136_0_0_1"/>
    <dbReference type="OrthoDB" id="2191195at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="144" mass="15984" checksum="28E4A344AB67AF9D" modified="2012-10-03" version="1">MKKIYLTLCAILGAMKSFYLFHSVVFILGIGVTSIGVVDARNLKPVGIGNDIHPRGPVTNTVPEAIRGPGVVAMLEENCSHARQLENKLKELGVPYKPLYAEENLDVYNFMTTQDGGPLLYEDGKRVEDIEKFLKEYKAKNGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFY9</accession>
    <name>I7AFY9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090170</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265132.1">
        <property type="nucleotide sequence ID" value="XM_009266857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFY9"/>
    <dbReference type="GeneID" id="20521956"/>
    <dbReference type="KEGG" id="ero:EROM_090170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090170"/>
    <dbReference type="HOGENOM" id="CLU_653871_0_0_1"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF36">
        <property type="entry name" value="CASPASE FAMILY P20 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="129"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="419" mass="47898" checksum="0E0DF1B6F9B89EED" modified="2012-10-03" version="1">MGNAASNIRKDPIIAKSNPSAPGVLRLYNLEDHVFPYKVSKSLETYPSVHLASSLASFEKLALKHLPLSPRPEEITGKEADIYHNVVNREETAREILASGERMTVIRHNLKEDKNQVYMCRQFVVEITEGIGEIQDLKVLQACCNYLTRLPQQIGCLTNLKVLVLSKNRIQKLPDEIGQLKNLRELNLSQNLLVTLPRGISALKALNALHIDDNLFTVLPPVIGRLYGLKYLNISNNKIQNIPLEILKLPFLIELTASSCDFTSKDSIECIGDITLKETCSRHLVRNNLRVRRNIHRSLIGYLLSVQECSFCGGPLFEHYYLHKSTQTFDSERFPIAYKLCVKHYVRHEDRIATLFLGPLKTYPQRLIKSNMPFVSELFNHFGYNDEQKRILEQGWNSEEGKMPLICLSKYARMHDEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG57</accession>
    <name>I7AG57_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative divalent heavy-metal cationtransporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_091140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265227.1">
        <property type="nucleotide sequence ID" value="XM_009266952.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG57"/>
    <dbReference type="GeneID" id="20564338"/>
    <dbReference type="KEGG" id="ero:EROM_091140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091140"/>
    <dbReference type="HOGENOM" id="CLU_040462_3_0_1"/>
    <dbReference type="OrthoDB" id="448280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF44">
        <property type="entry name" value="PROTEIN ZNTC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="110"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="315" mass="34532" checksum="73453F9731260672" modified="2012-10-03" version="1">MNIQVILFAVIIFSSSLLVCLSPKIITSLKVVRKVFPFLTLLTAGFLLGVQLLELSPHMVSDCHEHKGHNHGHHTHESPMLGFFTAGLSFIFLLAIDTIVLKHKHCEEEEKKKHTHDTECHGNDPHRRISTRGNNHVPKPGEKALDTPASGKEAERLGCCDPSGAIKNTSSRTQVFIYILGISIHSFFEGLAFNSIDKIGSLEMGLILHKVLESFALGVPLFTSGFNFSTGLILALFYSSLTPIGIMIGSAPGFFGHTIKNIFKGLALGSIMFMVSIEMIPPMFNHPKVSRIHGILTLLIGYLSSAVVIHCSHPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG64</accession>
    <name>I7AG64_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265237.1">
        <property type="nucleotide sequence ID" value="XM_009266962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG64"/>
    <dbReference type="GeneID" id="20564348"/>
    <dbReference type="KEGG" id="ero:EROM_091240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091240"/>
    <dbReference type="HOGENOM" id="CLU_1288888_0_0_1"/>
    <dbReference type="OrthoDB" id="10264655at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028758">
        <property type="entry name" value="Cyclin, C/H/G types"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="214" mass="24898" checksum="E0A8EEBCB3BFDF8E" modified="2012-10-03" version="1">MGLEYMECKNLFETKNIRDIVSISRLLGMPQKTTYFTLAIYYRYILMMKSRKTIPIGAACILLSGKVNGSLRSLEHILKASYKHYDVDPEKFFQEDYKDAIDIELHACIAMDFNFEMEDPYGFLEKLCMDNDIDRSRAQTIWVMLNDTMYLPFILSFSIRSIVVSCIFISDVARGNGCEDIDEFKGRYKLSEINTEDIDFISREIVSFYECASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGA1</accession>
    <name>I7AGA1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265282.1">
        <property type="nucleotide sequence ID" value="XM_009267007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGA1"/>
    <dbReference type="GeneID" id="20564393"/>
    <dbReference type="KEGG" id="ero:EROM_091690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091690"/>
    <dbReference type="HOGENOM" id="CLU_2527450_0_0_1"/>
    <dbReference type="OrthoDB" id="275715at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056326">
        <property type="entry name" value="ISD11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23511">
        <property type="entry name" value="Microp_apicomplexa_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="84" mass="9972" checksum="FBF5642690342E2A" modified="2012-10-03" version="1">MLPESVNNLYKNLESVILQFKSPAFGSYFLKKAKDEYNDIYTRSCGKKDERAIERYLKDQEELLDILRRQTTIYNMFYDDSSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGL3</accession>
    <name>I7AGL3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polyadenylate-binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_100990</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265412.1">
        <property type="nucleotide sequence ID" value="XM_009267137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGL3"/>
    <dbReference type="GeneID" id="20564529"/>
    <dbReference type="KEGG" id="ero:EROM_100990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100990"/>
    <dbReference type="HOGENOM" id="CLU_012062_22_6_1"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="11"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="106"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="213"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="415" mass="47089" checksum="8329304B341B8193" modified="2012-10-03" version="1">MRKDERPPETGNIFVKNLPESFTSKDLDDAFSMFGEIVSCKVATTPQGKSKGYGFVQFKEKKAAKKVIKNFNNLNGLMLGGNKIAVELYNPDMKKGESKKVSTMFTNCFIKNFPVDTTEKELLELLVRYGKVTSLYFPIKDDGKPKGFAFANFESHESALNAINNLHGAFPFDSSKDGGEPFYIQKGQRKEERAEELRKMFEQLSMQGQSYRKNLYITNIPEGFGAEELNNIFKEFGSITSMSVGIDGANSQKQYAYICYSTPEEASIAVERGNEIYLDGNRLQVSYFKNKLERMKEKEFGGLGYKPGIPYMYNQGVNFTSNGYKRERSRSGTMRPYDNEFEKLHSLVLAAAPSFKSQWKSFGVNNEAEFSNKVTRAFRNKPEEEIKNMINLNFVLTKNIAAVIEDDNSNDQVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALA7</accession>
    <name>I7ALA7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Subtilisin-like serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_010950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263936.1">
        <property type="nucleotide sequence ID" value="XM_009265661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALA7"/>
    <dbReference type="GeneID" id="20520722"/>
    <dbReference type="KEGG" id="ero:EROM_010950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010950"/>
    <dbReference type="HOGENOM" id="CLU_011263_1_6_1"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <feature type="domain" description="Inhibitor I9" evidence="8">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="7">
        <location>
            <begin position="139"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="464" mass="51012" checksum="F35D577C3866282C" modified="2012-10-03" version="1">MILAITFLSLVLCRERSDYIVMFDQDPSMDKGSMNALYSMNILKAQSILEPDESIEIKITNGFVARMTKSTAEKMKRHPNVKMVVRDTPVGISGLKIGTSYNNSGEGILIQRHAPWGLSRIVGSVSFASGSYYYPMNSGEGVDVYVLDTGIEVSHPEFEGRARWGANFVAESLNEDEHGHGTHCAGVIGGKNFGISKKSSIIAVKVLDRYGSGMTSRLLQGLDFVIKEHEKKKDELYNAAANEYLDSTRSSDIDIEIDDSESFSFLQSKVPSVQRLVDTISEKALEPKTIINLSVGGFRNPALNFAIEYASRLGIHFSTAAGNEHEDACDFSPGSSKASITTGASTYKDTVAFFSNFGECVNIFAPGVDILSSWIGGTQKIVSGTSMAAPHTTGVMAAYLTYYDYDPHTLKKRIINDARIVEESSGNSRNDNIPWPFPALFNSNRRKLPMLSMENLLKRIKNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALY7</accession>
    <name>I7ALY7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA mismatch repair ATPase MutS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_030430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264161.1">
        <property type="nucleotide sequence ID" value="XM_009265886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALY7"/>
    <dbReference type="GeneID" id="20520954"/>
    <dbReference type="KEGG" id="ero:EROM_030430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030430"/>
    <dbReference type="HOGENOM" id="CLU_002472_10_1_1"/>
    <dbReference type="OrthoDB" id="295033at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03243">
        <property type="entry name" value="ABC_MutS_homologs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="entry name" value="MutS, connector domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011184">
        <property type="entry name" value="DNA_mismatch_repair_Msh2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF34">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MSH1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005813">
        <property type="entry name" value="MSH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="617"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="714" mass="81521" checksum="A1D51D1AA097F074" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM09</accession>
    <name>I7AM09_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264191.1">
        <property type="nucleotide sequence ID" value="XM_009265916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM09"/>
    <dbReference type="GeneID" id="20520984"/>
    <dbReference type="KEGG" id="ero:EROM_030730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030730"/>
    <dbReference type="HOGENOM" id="CLU_513900_0_0_1"/>
    <dbReference type="OrthoDB" id="2191687at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="440"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="491"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="532" mass="62073" checksum="8E00E8C28B5D3730" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM20</accession>
    <name>I7AM20_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAR-17a/AIG1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030930</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_009265936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM20"/>
    <dbReference type="GeneID" id="20521004"/>
    <dbReference type="KEGG" id="ero:EROM_030930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030930"/>
    <dbReference type="HOGENOM" id="CLU_103036_0_0_1"/>
    <dbReference type="OrthoDB" id="2190919at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="26768" checksum="5209A76BC275E3D1" modified="2012-10-03" version="1">MAKFRYLSASKEILKGVLLIFCVYGATDYSQPKEWVDFVNNLAGGSHIYITQLSLYMTIITLVLSYCVKHMGVSSIKEIYRDFLSITLPMEGLVTTVFWTLNSIDPTLLKNKELYTAGVRTPLICEVSLHLFPFIVLLVDQVGVNIYEKKRHYWFFGIFGFTYFIVIHHFQKLNDYWIYPFLGYMTILMRAVLVFTAVLLVVGYYKLFMQFSRVTNTRMYPKTLKKKLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Fungi incertae sedis</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
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    </dbReference>
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    <dbReference type="OrthoDB" id="2193563at2759"/>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="314" mass="36599" checksum="72BE91C8212C15FB" modified="2012-10-03" version="1">MDNVELYSTMRNMPGYRNTAIMMVFHLLDLLSKHLKMMVEHQHTPVIITEKVARYHYDGNYKNACEELKRGTYKAGEEMRRLLSNVEEWKKSTKRNFSYFQGYGGFESSVCKGFDKYLKGYFAKNNPAENIKKWNDMVEVVDFARSSWLNSDYKKHLWLKELNSKVLCSPEILLLLNGKLCFIPEWSFDNESYIAQIIQTELQNDPWAPAYYVEEAKDRYALITLVRLLLIATEIKDAVKPSWVETQELQSKLSRVERKLEFEKAYNRCKTEEEKMKLDTAILLVDAADTFGVIELSAAIFFISCVVAAFMIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMI7</accession>
    <name>I7AMI7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264421.1">
        <property type="nucleotide sequence ID" value="XM_009266146.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMI7"/>
    <dbReference type="GeneID" id="20521223"/>
    <dbReference type="KEGG" id="ero:EROM_041600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041600"/>
    <dbReference type="HOGENOM" id="CLU_2061459_0_0_1"/>
    <dbReference type="OrthoDB" id="2192201at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="13130" checksum="79D00B89A89A0CF8" modified="2012-10-03" version="1">MERKLPTVVTIGGRRYVKNNLNKGAGDGKVRLKEAIKSLGRLVHKSQISYCSINTKERLQIYRLDDSKVILNKKNGVVGFIVTGKAQNIDKGIYREPSNVVNSNVEASGDGAAEEREKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN95</accession>
    <name>I7AN95_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DEAD box ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264711.1">
        <property type="nucleotide sequence ID" value="XM_009266436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN95"/>
    <dbReference type="GeneID" id="20521518"/>
    <dbReference type="KEGG" id="ero:EROM_061220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061220"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_1"/>
    <dbReference type="OrthoDB" id="2194948at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF8">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="38"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="193"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="465" mass="54058" checksum="EB344E0DA66ABB5F" modified="2012-10-03" version="1">MERSVKIKGGSFKTMGLDYRLLRNISYENPTPIQRKTIPLVLERRSLMGVGRTGSGKTLCYLIPAVQRAIEGERTLVIVPTKELTTQVRRVLKKLSRGMEDVWKFIEISTPGNIQSTNADLLVVDEIDRILEEPSLRSVFDKVSEELTCQKIFFSATLPDHPLNMKVVEIEAKINETVEHFFFYVPSESKEAALLQILDKSKKTIVFVATKYAVELLLEILNKNNFESHGIYSSMDDEARRSNFRDFNASKTGILVVTDVAARGLDIPCLDTVVNYDLCDERTFLHRVGRVRGMGVQYSFVTYTDVFHFFNIQETYLPNVEIGTIPQEYLDVYDFKEFEHLRSVANRGHQKCLEFRRKVSVPAEYKDRIKLFKTHSMFEHRETLLDKLKKMNNGKTTCEREKEKPENKEYRDQGFIPYTRKDSRIHSSAFGVAKDDYIRERKEKFSFSVMRRKTMKKKTMQTNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANN7</accession>
    <name>I7ANN7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cation-transporting ATPase</fullName>
            <ecNumber evidence="13">7.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_071430</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.-" evidence="13"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264891.1">
        <property type="nucleotide sequence ID" value="XM_009266616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANN7"/>
    <dbReference type="GeneID" id="20521706"/>
    <dbReference type="KEGG" id="ero:EROM_071430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071430"/>
    <dbReference type="HOGENOM" id="CLU_001828_0_0_1"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047819">
        <property type="entry name" value="P5A-ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF8">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12409">
        <property type="entry name" value="P5-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="922"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="990"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1022"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1056"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1100"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="P5B-type ATPase N-terminal" evidence="15">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="231"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF12409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1142" mass="129030" checksum="7173B82BAAC0657D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANS9</accession>
    <name>I7ANS9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264941.1">
        <property type="nucleotide sequence ID" value="XM_009266666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANS9"/>
    <dbReference type="GeneID" id="20521758"/>
    <dbReference type="KEGG" id="ero:EROM_080220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080220"/>
    <dbReference type="HOGENOM" id="CLU_034979_1_0_1"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="227" mass="26433" checksum="E397CBD96EDE388E" modified="2012-10-03" version="1">MVKKFPKLKVLLLSISMIIVLRNVRRLRKSSLIPGRYLLLIAHPDDESMFFAPSLLNLKGNIDILCLSNGNKEGKGKEREEELRKVGYYIGANVTITTLFSDGKDWDPLKVYLRLLWAYMSRPFDVLMTFDERGVSGHKNHISCYKGATLFLRVHNDVKGAILESKSLFRKYIVDTGFSKVSSTVPFRMYMAPVKMILFHRSQMIWFRYLYMLFSNFMSYNDFTIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANW8</accession>
    <name>I7ANW8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MRP-like ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080700</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264986.1">
        <property type="nucleotide sequence ID" value="XM_009266711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANW8"/>
    <dbReference type="GeneID" id="20521806"/>
    <dbReference type="KEGG" id="ero:EROM_080700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080700"/>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="26067" checksum="E7541270A92FF694" modified="2012-10-03" version="1">MVMIAVMSGKGGVGKSSISIMLSTMMSERRKTLLLDFDLCGPSITSGFGIRENIFKGEKGLIPAKVTESLYILSMALLMKDDDSVIWRGPKKMSVLSMFYESIDGFSNVVIDMPPGISEEHGFLIGKDISTLIVTTPQNISLEDSSRAIDFCTSNGIEILGLIENMSGYCCECCGSFTNIFGSKGGERLAKEMGIPFVCRLPVDPLLCEALDEGGFTEKCESADAYIRFKRSILEIMNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP24</accession>
    <name>I7AP24_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265051.1">
        <property type="nucleotide sequence ID" value="XM_009266776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP24"/>
    <dbReference type="GeneID" id="20521873"/>
    <dbReference type="KEGG" id="ero:EROM_081380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081380"/>
    <dbReference type="HOGENOM" id="CLU_2061463_0_0_1"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02066">
        <property type="entry name" value="GRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="31"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="13637" checksum="513A83FD69402575" modified="2012-10-03" version="1">MPVMYGALNTGGDLGEDTTGYKGVISQEKCTMFVKRFCPYSIEARKLLHKRGISCKVIEVDYSPEAHDFAKRYQRTFPVFFLNDTLIKGGYEKLKYLSDRNLPPFDNSPLPIDRRSYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP55</accession>
    <name>I7AP55_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265086.1">
        <property type="nucleotide sequence ID" value="XM_009266811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP55"/>
    <dbReference type="GeneID" id="20521908"/>
    <dbReference type="KEGG" id="ero:EROM_081730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081730"/>
    <dbReference type="HOGENOM" id="CLU_1704222_0_0_1"/>
    <dbReference type="OrthoDB" id="2195403at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="16721" checksum="158B644A14CD6D6B" modified="2012-10-03" version="1">METGLGLKSFVFSLSNIILVAKVGLVILGTVYPCLYGLVALISLVVIQRKTIHKALGILLIIIYLKLLLGSSFEFDAPSKICNSTFSDEVDDQEADEFSSSSEHFIDDKIFPSEVKKNENIIGHRLEESNLSHLQNRDDARCRFCSRVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7API3</accession>
    <name>I7API3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Homeodomain-containing transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091380</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265251.1">
        <property type="nucleotide sequence ID" value="XM_009266976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7API3"/>
    <dbReference type="GeneID" id="20564362"/>
    <dbReference type="KEGG" id="ero:EROM_091380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091380"/>
    <dbReference type="HOGENOM" id="CLU_128308_1_0_1"/>
    <dbReference type="OrthoDB" id="2194933at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF5">
        <property type="entry name" value="HEMATOPOIETICALLY-EXPRESSED HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="37"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="39"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="132" mass="14964" checksum="BFC90654BA4C1EA5" modified="2012-10-03" version="1">MKDTRWVMEGEAMAGLVKLRRLSEKSYLGLIGYKYKTRTQVDVLKKIFEITHYPSHDTRLNLAILLNISPRTIQIWFQNSRSVSRGAKKKGSPSGQGSPGKPKIKIINSLTIPVKYIIWLILNSSSYSQIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7APS9</accession>
    <name>I7APS9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265381.1">
        <property type="nucleotide sequence ID" value="XM_009267106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APS9"/>
    <dbReference type="GeneID" id="20564498"/>
    <dbReference type="KEGG" id="ero:EROM_100680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100680"/>
    <dbReference type="HOGENOM" id="CLU_1948792_0_0_1"/>
    <dbReference type="OrthoDB" id="2192317at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="129" mass="14844" checksum="764724DDC39E24AD" modified="2012-10-03" version="1">MLVKDKLEMATTFFALILVIFIAPRYFYAGQVLISMVTYSAFVSLIKPVARRGKLYFRIINVLHVIAKILTLIAFFIVMNNVFVMRSGYKNCSECSIMSTANIFISILFFIYYSVYSTAFVFFFSVSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7APV4</accession>
    <name>I7APV4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265406.1">
        <property type="nucleotide sequence ID" value="XM_009267131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APV4"/>
    <dbReference type="GeneID" id="20564523"/>
    <dbReference type="KEGG" id="ero:EROM_100930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100930"/>
    <dbReference type="HOGENOM" id="CLU_2061460_0_0_1"/>
    <dbReference type="OrthoDB" id="2192730at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="118" mass="13367" checksum="4B0EEE73ED346C82" modified="2012-10-03" version="1">MEEGRKRKARDQLSPYSNPGEESDHHSPAVESSPLPNRKRAKARVGDLSTIANSETLKGVLSSVNILQPKVKNDRISGLNKCFLNAVQKVVEKESNKSLLYLFKQYEKYYEELKESNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQE2</accession>
    <name>I7AQE2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264029.1">
        <property type="nucleotide sequence ID" value="XM_009265754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQE2"/>
    <dbReference type="GeneID" id="20520817"/>
    <dbReference type="KEGG" id="ero:EROM_020570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020570"/>
    <dbReference type="HOGENOM" id="CLU_569899_0_0_1"/>
    <dbReference type="OrthoDB" id="2192194at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="393"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="479" mass="52892" checksum="A5B6AE742D7CD651" modified="2012-10-03" version="1">MGKDTLYSYLSPITDEEEGKFRDPRGLSMGSMNEKLRKLNEEFKARSCIGNGFVFNRNIRLESPVSYFGKRRDFPDGNESINNGQRCQRDAGDANSPKAKDGFIGGMISWKVNPSGGINRSMVHKEMSRGSVVGMDLYKKPSIDFTSYTMKKGSGASSKGYVKSMASSSIFNRGPSSNPGGIGKERSVDPSCLYIKKKEQVEEKSSPSRSVFSNPLSNSRTSSSSLKVSSQASSSFIHNSTSRSPALAPIDVMYVKTSEPRNGMPNMGDGDSEPSQLWNDPSNTSPVSNLYAPGRKLTIHPSIRISNTVLGYEGSAETTKYYIMVESDVNWMICKSIGDIAALIPGIRCTTLSNAMSPQERRARDRMIQVTLNSGITDKQLQSFILSDISSVQVFRSSYLLMDNEGWKAYLFKFVGKALICYEKSRVFKILLLSGCNVLPVGQVAFCLEKSGEGIELYATCEKERDAWISDIKEYISKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQF7</accession>
    <name>I7AQF7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_020720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264044.1">
        <property type="nucleotide sequence ID" value="XM_009265769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQF7"/>
    <dbReference type="GeneID" id="20520832"/>
    <dbReference type="KEGG" id="ero:EROM_020720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020720"/>
    <dbReference type="HOGENOM" id="CLU_089419_2_2_1"/>
    <dbReference type="OrthoDB" id="10262490at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF8">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN RLP24-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="TRASH" evidence="3">
        <location>
            <begin position="6"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="18744" checksum="D31D491FD3EFABE8" modified="2012-10-03" version="1">MRIEKCWFCSSNIYQGHGTVYVRNDAKVFRFCRSKCRKFFERRVNPRRVKWTKIARKMANKELCNDTVLTFERRLNEPRIYDRGVVEKTLSTIPKILEIRKRREDFFIKDRILTGQEMTKESDLKYIERHANLLEEEISSEKHVAKTKKKEPQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQH2</accession>
    <name>I7AQH2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane integral protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264059.1">
        <property type="nucleotide sequence ID" value="XM_009265784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQH2"/>
    <dbReference type="GeneID" id="20520848"/>
    <dbReference type="KEGG" id="ero:EROM_020870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020870"/>
    <dbReference type="HOGENOM" id="CLU_115931_0_0_1"/>
    <dbReference type="OrthoDB" id="759142at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009038">
        <property type="entry name" value="GOLD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01105">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01190">
        <property type="entry name" value="EMP24_GP25L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="GOLD" evidence="2">
        <location>
            <begin position="24"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="200" mass="22943" checksum="A23C72D4FC2BF8F6" modified="2012-10-03" version="1">MIFVLYLGMIVGEIFSARRNRNIEIEFDVIENKICKGYLKPINCKNSSFKAVLASDTGSVYLSNENLPVDTETHFSFNISEPKVLSMVLTPIVIDDSQPHTFGEIEVDFSTQFDTFKKDVAKKVSVEPAMAAMTKLDNLLHELNSESEYLAHRMGSIDYEHRRMFSFVTVFSVVTLVIYFGVNSYELYSLKKFFQKKKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQL4</accession>
    <name>I7AQL4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Vesicle coat complex COPII subunit Sec24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_021370</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264109.1">
        <property type="nucleotide sequence ID" value="XM_009265834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQL4"/>
    <dbReference type="GeneID" id="20520899"/>
    <dbReference type="KEGG" id="ero:EROM_021370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021370"/>
    <dbReference type="HOGENOM" id="CLU_026726_0_0_1"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007123">
        <property type="entry name" value="Gelsolin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00626">
        <property type="entry name" value="Gelsolin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="3">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="5">
        <location>
            <begin position="393"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="4">
        <location>
            <begin position="500"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Gelsolin-like" evidence="2">
        <location>
            <begin position="604"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="713" mass="80983" checksum="CA96240C3AAB5029" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR80</accession>
    <name>I7AR80_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">T complex protein 1 theta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_041000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264364.1">
        <property type="nucleotide sequence ID" value="XM_009266089.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR80"/>
    <dbReference type="GeneID" id="20521164"/>
    <dbReference type="KEGG" id="ero:EROM_041000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041000"/>
    <dbReference type="HOGENOM" id="CLU_1166130_0_0_1"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="212" mass="24122" checksum="20DBB71BC568D5A0" modified="2012-10-03" version="1">MQREGDKIYLDFLNKSRKLVFRITSKYDLRRIRELLGGHILSSLGSQSEESMGFVEEVVTFTEGICEYTKFISGSKKVYTLVLKNSVQAELDEQERMVQKALNVLSKNTKENKIDLVDGAGRFEKRLSKIFLERSAASKEGKGLVYKCIGKVLGGFGSSDAEIYDVYNSKVKALKYSIEFISTLFETSDYLIGKPEAMNITPRNNQNWDEDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR92</accession>
    <name>I7AR92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Eukaryotic translation initiation factor IF1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264379.1">
        <property type="nucleotide sequence ID" value="XM_009266104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR92"/>
    <dbReference type="GeneID" id="20521179"/>
    <dbReference type="KEGG" id="ero:EROM_041160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041160"/>
    <dbReference type="HOGENOM" id="CLU_109098_1_1_1"/>
    <dbReference type="OrthoDB" id="274995at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="2">
        <location>
            <begin position="11"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="13488" checksum="E4FD54FD3A0C5554" modified="2012-10-03" version="1">MKGRKGRFTNDRALVFAEEEGTVYGQIICPLGQGQFKVSCSDGIYRTAKVRGRDYRRVRMIPGDIVLLRVRDGDEKRADIDRKYMPKEIKILKEDGEINDDTFAANDGGSGLVEFEELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARA9</accession>
    <name>I7ARA9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264399.1">
        <property type="nucleotide sequence ID" value="XM_009266124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARA9"/>
    <dbReference type="GeneID" id="20521199"/>
    <dbReference type="KEGG" id="ero:EROM_041360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041360"/>
    <dbReference type="HOGENOM" id="CLU_2831186_0_0_1"/>
    <dbReference type="OrthoDB" id="10359876at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="66" mass="7322" checksum="3536390F66633870" modified="2012-10-03" version="1">MVNVCLLTVTSTIAYGCVFYTLVIRPLFYDPTFFENALIILSSIILSSLLLIHAILGFDGENIKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARP2</accession>
    <name>I7ARP2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Polynucleotide 5'-hydroxyl-kinase GRC3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polynucleotide 5'-hydroxyl-kinase grc3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_051160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264544.1">
        <property type="nucleotide sequence ID" value="XM_009266269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARP2"/>
    <dbReference type="GeneID" id="20521348"/>
    <dbReference type="KEGG" id="ero:EROM_051160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051160"/>
    <dbReference type="HOGENOM" id="CLU_018195_1_1_1"/>
    <dbReference type="OrthoDB" id="258143at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1030">
        <property type="entry name" value="Clp1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.330">
        <property type="entry name" value="Pre-mRNA cleavage complex subunit Clp1, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010655">
        <property type="entry name" value="Clp1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038238">
        <property type="entry name" value="Clp1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032324">
        <property type="entry name" value="Clp1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038239">
        <property type="entry name" value="Clp1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF6">
        <property type="entry name" value="POLYRIBONUCLEOTIDE 5'-HYDROXYL-KINASE CLP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06807">
        <property type="entry name" value="Clp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16573">
        <property type="entry name" value="CLP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Clp1 N-terminal" evidence="6">
        <location>
            <begin position="5"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 P-loop" evidence="7">
        <location>
            <begin position="106"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Clp1 C-terminal" evidence="5">
        <location>
            <begin position="270"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="373" mass="42432" checksum="B239836968FE9A5A" modified="2012-10-03" version="1">MEIRLWPNQEFRIEVNETQKLKVMVVSGLAEVKGQELLNEKWYAFKDTKTFIFTFSGCKLKVEGACDLQYVSDTTNVPLIFNILSFFSKNGFDYSKLRTFMVIGNGRSTFCFTLINFFVRMGKKVLFTEVDPSRGNIFPGALSTIHVDTLVDCIEGLRLKNPLSFYYGSTEITNMELYDLQTTKLQEAIKSKGVEDLHLILCPEGTSAFYNTLIKRFEVDRVIVVGNERLYHSLDTIAEKLMINRSGFVEEKEVGRSISRYFKGVNNEYTPFTFNVKYKWRIVRIGEMYVAPVSALPLGSTRKVGCVEASEVEIGENSVLGISEAREIEDVAGSPIIGYVVVIDANTFKILCTQPRLPKYTFLVQGDLKHIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARQ3</accession>
    <name>I7ARQ3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">t-SNARE complex syntaxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264554.1">
        <property type="nucleotide sequence ID" value="XM_009266279.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARQ3"/>
    <dbReference type="GeneID" id="20521358"/>
    <dbReference type="KEGG" id="ero:EROM_051260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051260"/>
    <dbReference type="HOGENOM" id="CLU_093580_0_0_1"/>
    <dbReference type="OrthoDB" id="10251371at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="146"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="234" mass="27173" checksum="33E0D8E9259ED4CB" modified="2012-10-03" version="1">MLVNRTLEYKRLTKRKDIKFSPLSTSSLRGSLDDVKQSISRLGIIQEKHCMPSFSPKKKKKDEIDEVKRHITDQIHRAEKGIEEIVKFSNSKVLTDCMHGYFAGQLKAIIHDYRGLQQKFLKNIDIYDEIDHEDEENDGGSMLLLENVKDLRKSIYDLTSVLLDMKMAVGQQSLQIDRLDFYLDSINFYLEGANKELGKIPGSHRRMKNKVIYFMLLLSIVLVLMSMLKMARSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS86</accession>
    <name>I7AS86_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_070310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264779.1">
        <property type="nucleotide sequence ID" value="XM_009266504.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS86"/>
    <dbReference type="GeneID" id="20521590"/>
    <dbReference type="KEGG" id="ero:EROM_070310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070310"/>
    <dbReference type="HOGENOM" id="CLU_1185004_0_0_1"/>
    <dbReference type="OrthoDB" id="2189106at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3630.10">
        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008628">
        <property type="entry name" value="GPP34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038261">
        <property type="entry name" value="GPP34-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05719">
        <property type="entry name" value="GPP34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="234" mass="26669" checksum="9618007A8FF15919" modified="2012-10-03" version="1">MELMSTRKREYSKHVESKGGLKLYEQLVVLLRTRSEIKFLCNRVSLSIRACIVVELALCGAIGLKEGRVVGKDNTLNSSLLLEAMSKIYQTQLPPDELMYRLNGEKRGGSIYMRNVRLRVYKDLEEKRVCKVESRSLIFNKITMNDYNIRIELINYIKEYLTSSKGVDYRAEALVVCLIFCSAIDSILICMTEKEAAASSAHINRIKEKYMTKSTCRPGNEMIIYSVLGALLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASF0</accession>
    <name>I7ASF0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264854.1">
        <property type="nucleotide sequence ID" value="XM_009266579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASF0"/>
    <dbReference type="GeneID" id="20521666"/>
    <dbReference type="KEGG" id="ero:EROM_071060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071060"/>
    <dbReference type="HOGENOM" id="CLU_1481976_0_0_1"/>
    <dbReference type="OrthoDB" id="2189208at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="182" mass="21298" checksum="32411141B0289824" modified="2012-10-03" version="1">MPVKEKDEVLLEDIKQDLQSIRTAVIESGDISKLDEYNLEGLFGIYKNPEQQVVFMRTIYKQITREILKFLSSKCISEAMVSICILYEMFKSQRGYIDVEMSLEDVECINQYVYSLGKKENTDIIWEGVRAWGCLKRKKAIRICMMPFPDDEQSLMVIEKELEVISKTLDNWDEPRNESCID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASQ3</accession>
    <name>I7ASQ3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264969.1">
        <property type="nucleotide sequence ID" value="XM_009266694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASQ3"/>
    <dbReference type="GeneID" id="20521787"/>
    <dbReference type="KEGG" id="ero:EROM_080510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080510"/>
    <dbReference type="HOGENOM" id="CLU_053603_2_2_1"/>
    <dbReference type="OrthoDB" id="2190313at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007590">
        <property type="entry name" value="Saf4/Yju2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12111">
        <property type="entry name" value="SPLICING FACTOR YJU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04502">
        <property type="entry name" value="Saf4_Yju2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="144" mass="16195" checksum="79289AA0F2BB1166" modified="2012-10-03" version="1">MKSTANRKKESKSKGAATRLATPFTIKCLSCGAYIYKNKRHNATRQVAQGKDYLGVESHRFYIKCTGCNETLSIRTDPKNGIYITENGCVRIDGEDNIQDKIEDSSIQLKKESDMKDEIIKLRMQTSYVNSSNSTLDGSDKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASW7</accession>
    <name>I7ASW7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265039.1">
        <property type="nucleotide sequence ID" value="XM_009266764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASW7"/>
    <dbReference type="GeneID" id="20521861"/>
    <dbReference type="KEGG" id="ero:EROM_081260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081260"/>
    <dbReference type="HOGENOM" id="CLU_1069968_0_0_1"/>
    <dbReference type="OrthoDB" id="2189706at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="33652" checksum="E9126D6E9FFE9784" modified="2012-10-03" version="1">MEDASYLCDLSFYSSSSAGDSLIASDKMGVVHIVAPVMSTMSVDHFKKDIKFYPTDDLCFTTADMFKGIRVWDRCLGEVVYSYKEDSIKMHAYSRTGCLGAVGEGCVKLYDLRVRYNIDAIPLKMCWKAEWGEDKIYCINDECVVEYDTRNMSSMMCSEKSEAQLKKKDIEGILDFASTSKGEFCTVLREEVTYLMRISGLEVMEGTRRTSVGGKIIKIEDSFDDFVIGTVNGSTMGFYEYKDTWTHKFDGLTNVDWMWFGKNKTYMFADRKVYFMVGGYERFKTAMKGGPDTAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASY1</accession>
    <name>I7ASY1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_081410</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265054.1">
        <property type="nucleotide sequence ID" value="XM_009266779.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASY1"/>
    <dbReference type="GeneID" id="20521876"/>
    <dbReference type="KEGG" id="ero:EROM_081410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081410"/>
    <dbReference type="HOGENOM" id="CLU_1102768_0_0_1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ATL42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46539:SF9">
        <property type="entry name" value="RING-H2 FINGER PROTEIN ATL56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="188"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="249" mass="28488" checksum="327F39ABD1435F84" modified="2012-10-03" version="1">MSVYRIGAYVILQMFDVSVYMPLYAIHGDAHLGEKLTLNDVKIEAKNSPDLFRHLSRNNEEEKDYYVDSLEIYKIEGKEEVDRLNGRVSKDVIFGTMAFDFSMLKAILSQETQKNTGHSPEIRIKSIEIRVPLAIQIFLHIPIVLVAVIVFYLFLSFGAEEGGGLSEKEIEKIPLCPYSSQEFISRGCIICLEDFEDGGCVRNLGCGHVFHRECIDKWLRKNFVCPVCRSRMTLGCGKQGYERRRVHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT22</accession>
    <name>I7AT22_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_081870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265099.1">
        <property type="nucleotide sequence ID" value="XM_009266824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT22"/>
    <dbReference type="GeneID" id="20521922"/>
    <dbReference type="KEGG" id="ero:EROM_081870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081870"/>
    <dbReference type="HOGENOM" id="CLU_1875411_0_0_1"/>
    <dbReference type="OrthoDB" id="2194068at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707429" evidence="2">
        <location>
            <begin position="18"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="134" mass="15286" checksum="9C6A97830272137F" modified="2012-10-03" version="1">MCLTVLIFCMGIHAISSDEELKDAIRATIKEEVTKEMHDTVSRITNDMREDLEKRIDYLISGKEAEEEIAIETPVQDTEEEGEETSAVFISKKVGEGLGIGVISILIVYLVRKIFEYYRRLKDCSELDEDHSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT44</accession>
    <name>I7AT44_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="8">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_090040</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265119.1">
        <property type="nucleotide sequence ID" value="XM_009266844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT44"/>
    <dbReference type="GeneID" id="20521943"/>
    <dbReference type="KEGG" id="ero:EROM_090040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090040"/>
    <dbReference type="HOGENOM" id="CLU_020802_3_0_1"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="436" mass="49570" checksum="2EE1C821A627DC1F" modified="2012-10-03" version="1">MKKTLSEVELFGITSITHLSILIYHLVFPRYALFEFLFSIIPLYLTIAFPNHLHLIYASFFAVFVMCFLFTKIQKWGPWMPTPVWPASIIDNPSSSVKLSISVVQDDEPYASVAIDRVRLLIMGCVAITIFISDFSFYEGSKLGKSMGNGLKLMDVGVGSFVYNAGFFSTKATPRRKVQNVLSSLFFGILRYLSKKLLDLGVSDTEFGVHLNFFLILGILNLASLFINTQIDFLIGFAMCLLHEILLKFFGLEELIYTTKRLNIITANIEGIAFILPQMGMFLMASGISKALFKKKNPGIIVLYNIFFVTILLGTRMYSMSCRRIHNLYFCMVIMLLHTTQGIACGASNKIFKIRDLKIHRFTSKYLLFILIWSNLLVGINKLFLNPATMPNYLAHGFCIAYLAVVFYLPYIAVNRNAMRDCKQVNSKIFSPELWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATA6</accession>
    <name>I7ATA6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265149.1">
        <property type="nucleotide sequence ID" value="XM_009266874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATA6"/>
    <dbReference type="GeneID" id="20521973"/>
    <dbReference type="KEGG" id="ero:EROM_090350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090350"/>
    <dbReference type="HOGENOM" id="CLU_2084827_0_0_1"/>
    <dbReference type="OrthoDB" id="2193071at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="117" mass="13062" checksum="7851964431F902BA" modified="2012-10-03" version="1">MEENITESGIPSTGFGGQEILIGEVPNEDAFVICPSQGNIDKKAVHSKDSSAIDDISKCKKHRVDNCICLSKIHYEVDGDILCAREMKPFFYINKVSILRLRRASCTKANTERQKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATB4</accession>
    <name>I7ATB4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histone H4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_090450</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265159.1">
        <property type="nucleotide sequence ID" value="XM_009266884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATB4"/>
    <dbReference type="GeneID" id="20521984"/>
    <dbReference type="KEGG" id="ero:EROM_090450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090450"/>
    <dbReference type="HOGENOM" id="CLU_109117_2_3_1"/>
    <dbReference type="OrthoDB" id="2532770at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22912">
        <property type="entry name" value="HFD_H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001951">
        <property type="entry name" value="Histone_H4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10484">
        <property type="entry name" value="HISTONE H4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00623">
        <property type="entry name" value="HISTONEH4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="9">
        <location>
            <begin position="36"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="103" mass="11369" checksum="844429B693D573B9" modified="2012-10-03" version="1">MNIPNIGAKGKSKAAKGIAKRHRKQSGLTDSISKPAIRRIARRAGVRRVGGGCFKEINNAAREYIRDTLSIACIYATHAKRKTITCSDILHSLKRMGIKYIGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATG0</accession>
    <name>I7ATG0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">5-3 exoribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_091160</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the 5'-3' exonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265229.1">
        <property type="nucleotide sequence ID" value="XM_009266954.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATG0"/>
    <dbReference type="GeneID" id="20564340"/>
    <dbReference type="KEGG" id="ero:EROM_091160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091160"/>
    <dbReference type="HOGENOM" id="CLU_006038_3_1_1"/>
    <dbReference type="OrthoDB" id="372487at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18673">
        <property type="entry name" value="PIN_XRN1-2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12390">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027073">
        <property type="entry name" value="5_3_exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041412">
        <property type="entry name" value="Xrn1_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004859">
        <property type="entry name" value="Xrn1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341:SF7">
        <property type="entry name" value="5'-3' EXORIBONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12341">
        <property type="entry name" value="5'-&gt;3' EXORIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17846">
        <property type="entry name" value="XRN_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03159">
        <property type="entry name" value="XRN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xrn1 N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="7">
        <location>
            <begin position="318"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Xrn1 helical" evidence="7">
        <location>
            <begin position="429"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="656" mass="75497" checksum="B1605F6FD0A555BE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUJ9</accession>
    <name>I7AUJ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111510</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265629.1">
        <property type="nucleotide sequence ID" value="XM_009267354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUJ9"/>
    <dbReference type="GeneID" id="20564750"/>
    <dbReference type="KEGG" id="ero:EROM_111510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111510"/>
    <dbReference type="HOGENOM" id="CLU_108559_1_1_1"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="137" mass="15915" checksum="F25DD2D1E8025E91" modified="2012-10-03" version="1">MIEEIYEVRPEKFNDALKSYLKSTNKVVPLQDYDIMKTGEGKEQAPIEDDWYFTRMASIVRQISIKGAVTPEFLAKRYGSRKNRGCRPSKYVNAYPEIGVSVLENLKNMGWINEHPKDMLTEKGRTVVKEIIEKVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGP1</accession>
    <name>I6ZGP1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263850.1">
        <property type="nucleotide sequence ID" value="XM_009265575.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGP1"/>
    <dbReference type="GeneID" id="20520634"/>
    <dbReference type="KEGG" id="ero:EROM_010080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010080"/>
    <dbReference type="HOGENOM" id="CLU_186268_0_0_1"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10652" checksum="2BE399A5F37DC20A" modified="2012-10-03" version="1">MKDNYEEDEGQVFTSRLKTLRNVAIDLTKEIEAQNIKLRQLEPSFQSSLNKIFTTIQSIRKSDPKRFRSWLYFILGGIGLSFLFFILFIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGU0</accession>
    <name>I6ZGU0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha,alpha-trehalose-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263900.1">
        <property type="nucleotide sequence ID" value="XM_009265625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGU0"/>
    <dbReference type="GeneID" id="20520686"/>
    <dbReference type="KEGG" id="ero:EROM_010590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010590"/>
    <dbReference type="HOGENOM" id="CLU_002351_7_2_1"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000010">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="459" mass="52606" checksum="EF456870B4A0D34C" modified="2012-10-03" version="1">MKLLVISNRLPLTIKKGKNGFEYTRTSGGLVTGLKSINDRIRFKWLGNISGVELNEEEKDIIRKDCWEKFHSIPVFIDPELNSDCYDGFCNAILWPIIHSFKDDVAFTIKNYNAYVEYNRIFCDKVCEALEDGDIVWVHDYHLMMLPEMLKKKSKKDFKVMFFLHTPFPPAEIMETLACRKEIVSGMAHSDLVAFHSFDYAINFHDTCIANHIEVKTKLDAIPIGIDPEMFRKILKEKKTVERIKEIKQMFKGKKILLGVDRTDYIKGMPHRFKGFHRFLEKYPEFVGKVVFLQVGVPSRTNVKEYSSYTNKLNELASEVNSKIGPIEATHIHFLNRSVEFDELCALYAASDVLLVTSLIDGMNLVALEYVSCQDENKGVLLLSESAGASTTLSAALEINSWNTEEIADGIQKAIMMPMEERSERHEINKKAVDVFTSVEWAERNLDGLCDGWRKSLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH89</accession>
    <name>I6ZH89_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transport protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264060.1">
        <property type="nucleotide sequence ID" value="XM_009265785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH89"/>
    <dbReference type="GeneID" id="20520849"/>
    <dbReference type="KEGG" id="ero:EROM_020880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020880"/>
    <dbReference type="HOGENOM" id="CLU_1525130_0_0_1"/>
    <dbReference type="OrthoDB" id="10262857at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18979" checksum="A7DB8E5020AD70BD" modified="2012-10-03" version="1">MSKRNEQQMSYDVFALVYTSFINKVVKDGVGKDREKMFRIGVRIGERLADDFFFTASPGKPMSLAEISKDVSETFFPHYFSFRPTYNQGIIALKKFPMLQYTRKEKKCLEMICGILKGVYNHVSKEDIWFEASEDSGEYCIIVRDAGCAMDPVVMKFSDDLGEKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHB3</accession>
    <name>I6ZHB3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_021130</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264085.1">
        <property type="nucleotide sequence ID" value="XM_009265810.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHB3"/>
    <dbReference type="GeneID" id="20520875"/>
    <dbReference type="KEGG" id="ero:EROM_021130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021130"/>
    <dbReference type="HOGENOM" id="CLU_005138_4_1_1"/>
    <dbReference type="OrthoDB" id="206088at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07900">
        <property type="entry name" value="Adenylation_DNA_ligase_I_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07969">
        <property type="entry name" value="OBF_DNA_ligase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000062">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000002">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF4">
        <property type="entry name" value="DNA LIGASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="16">
        <location>
            <begin position="331"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AFN82588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="589" mass="67074" checksum="0B117549F80A73A6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHK2</accession>
    <name>I6ZHK2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_030275</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Dispensable for SPC enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264145.1">
        <property type="nucleotide sequence ID" value="XM_009265870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHK2"/>
    <dbReference type="GeneID" id="20520938"/>
    <dbReference type="KEGG" id="ero:EROM_030275"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030275"/>
    <dbReference type="HOGENOM" id="CLU_134505_2_1_1"/>
    <dbReference type="OrthoDB" id="263893at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009542">
        <property type="entry name" value="Spc1/SPCS1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 12 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13202:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06645">
        <property type="entry name" value="SPC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="87" mass="9878" checksum="704C008707F92555" modified="2012-10-03" version="1">MYRSLLSKIDPPIDYVGQKLASDLMYLIFGVGYTAALIFGVSLNNLVYTMYISITTLVISAIVVVPSWGFYRRNPLKFRPPVKDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHR2</accession>
    <name>I6ZHR2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="2">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NCS6</name>
        <name evidence="2" type="synonym">CTU1</name>
        <name evidence="5" type="ordered locus">EROM_031110</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="2"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264230.1">
        <property type="nucleotide sequence ID" value="XM_009265955.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHR2"/>
    <dbReference type="GeneID" id="20521024"/>
    <dbReference type="KEGG" id="ero:EROM_031110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031110"/>
    <dbReference type="HOGENOM" id="CLU_026481_1_2_1"/>
    <dbReference type="OrthoDB" id="198857at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="4">
        <location>
            <begin position="51"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="314" mass="35155" checksum="D893EDD197CCED7B" modified="2012-10-03" version="1">MVCVRCNTSQAIVIRAKDRAKVCKSCFFEGFEVDVHETIVSSGMFRKGDKVGIGMSGGKDSTVLAYVLDLLNKRHGYGVELVLLGVDEGITGYRDQSIMSVCKNSSRLGLKLKIVSFEETFGTTMDKVVRKVGRRGNCTYCGVFRRQALEDAARKMGVDVIVTGHNADDMAETVLLNIVRGDISRLRRCTLARTKPQRSEDGRMISLSRLKPFKHIYQKEIVLYAFHKKLEYFSTECTYSPGASRGDLRVLMKQLEREDPKVILDVIRSGDIIQQEDSTHRSPIPCVLCGHSTSSRDAICNGCTLVRRLKQCKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHY8</accession>
    <name>I6ZHY8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dolichol kinase</fullName>
            <ecNumber evidence="3">2.7.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_040600</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polyprenol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.108" evidence="3"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264325.1">
        <property type="nucleotide sequence ID" value="XM_009266050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHY8"/>
    <dbReference type="GeneID" id="20521124"/>
    <dbReference type="KEGG" id="ero:EROM_040600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040600"/>
    <dbReference type="HOGENOM" id="CLU_921424_0_0_1"/>
    <dbReference type="OrthoDB" id="2192014at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004168">
        <property type="term" value="F:dolichol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043048">
        <property type="term" value="P:dolichyl monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032974">
        <property type="entry name" value="Polypren_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205:SF15">
        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707003" description="dolichol kinase" evidence="10">
        <location>
            <begin position="21"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="311" mass="35546" checksum="13AE46C182D9E3D2" modified="2012-10-03" version="1">MRSSLSYIILLDIVDLVVETASLETCRSTSQVPERWITAVILKCIEQKIQIRSPLLGVRYAIFSDPCKPLLAKISECLQIVNIPEYIFRTIDEMKQEPYAFIEVVCISLGCLLMQTFLFRSSWTNLKRKTFHFFAFLVFYKEYRMSLVLGEGLLLVLGLLSSCKYVNSLFKPYLSKNDKGRTVLSHIYLLSACIYPRLFIRHVEYVSVLVSICFQDSMASIIGEWLGKTEKSIQGSIGGTVSGTMVYFTLYRKIDMVPFFVFIGITEYLIPINDNVSIPLFAVLYFQILKRFNPSNNLSWGWVSLPSGSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIR1</accession>
    <name>I6ZIR1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_060180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264610.1">
        <property type="nucleotide sequence ID" value="XM_009266335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIR1"/>
    <dbReference type="GeneID" id="20521415"/>
    <dbReference type="KEGG" id="ero:EROM_060180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060180"/>
    <dbReference type="HOGENOM" id="CLU_053380_6_0_1"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="123" mass="14367" checksum="49CD275D5CD1AF23" modified="2012-10-03" version="1">MVVEQLFIINKSGGMVFKYEREENTDINSFLILTSSLYSVSVILSKVMDNPASRQVVYFRNRTISIFRTITGLVFVFVADKPADALFERVYSHYCKYVTRDPFYSPEMPIQCSKFRPHLLFEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIU4</accession>
    <name>I6ZIU4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WDR36/Utp21 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264620.1">
        <property type="nucleotide sequence ID" value="XM_009266345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIU4"/>
    <dbReference type="GeneID" id="20521425"/>
    <dbReference type="KEGG" id="ero:EROM_060280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060280"/>
    <dbReference type="HOGENOM" id="CLU_378988_0_0_1"/>
    <dbReference type="OrthoDB" id="2190571at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="1">
        <location>
            <begin position="35"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="731" mass="84221" checksum="39CC8AF986F60663" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ17</accession>
    <name>I6ZJ17_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264700.1">
        <property type="nucleotide sequence ID" value="XM_009266425.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ17"/>
    <dbReference type="GeneID" id="20521507"/>
    <dbReference type="KEGG" id="ero:EROM_061110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061110"/>
    <dbReference type="HOGENOM" id="CLU_080496_0_0_1"/>
    <dbReference type="OrthoDB" id="2192095at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="305" mass="35948" checksum="DDFE0A272A7A2889" modified="2012-10-03" version="1">MAYKRKRNVKIDNSQPYSIYKPGVSEASSLEEYIPPSLDTGMEADEEKELHLKKIMEGEKGSIPIPVIVEVYNPAREAYGRYTPRRRYVEWSGDAENKYLLSDEDKKICRERGISEEEFYKLLCTLSREQRLVEGKEGLIKMLSSRVLIRNENFSPLAYVCFRKRIIKPNRRSRRSEELSKEKVERMWKELYLLEVLCSLYCKRNELERDLENVESNLFQTSCLLIKSSSRRARKRIGRKVLNKQGGTSKDGPNATNGTIGGIIFDRTRIRLLRQRIRESKERVRMGEYDSEIQAYKKYNELYRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJF1</accession>
    <name>I6ZJF1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Frq1 calcium-binding caltractin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264865.1">
        <property type="nucleotide sequence ID" value="XM_009266590.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJF1"/>
    <dbReference type="GeneID" id="20521677"/>
    <dbReference type="KEGG" id="ero:EROM_071170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071170"/>
    <dbReference type="HOGENOM" id="CLU_2015249_0_0_1"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="122" mass="14330" checksum="3F284FBB47B97A70" modified="2012-10-03" version="1">MGTMKEQWDAFDTGKLTKETTKDLLRLCGFTPRERDMAVPRTFEEFSQLASTIPPPIPKEEMRRMVQMFIHGMHISRKNLGKYMTMGDKLNEEEMSELFRSCPFDRNGEITINELLDFLYDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK05</accession>
    <name>I6ZK05_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Cyclin-dependent protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_081770</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265090.1">
        <property type="nucleotide sequence ID" value="XM_009266815.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK05"/>
    <dbReference type="GeneID" id="20521912"/>
    <dbReference type="KEGG" id="ero:EROM_081770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081770"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="OrthoDB" id="204883at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000624">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF546">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="4"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="309" mass="35751" checksum="15EC065430E8A3D7" modified="2012-10-03" version="1">MEKYENIKQVGEGAFGQVYKSRRTSDGTVFAIKKMPVDRETGFPFTAIREIKLCKSVINKHIIKLDEIVFDEGFIFVVLEYMPYDLTGLLASGARLSTDHTRSIISQLVEAVSSMHELGLVHRDIKPSNILMDHRGMLKLTDFGLTREISGMMTNRVCTLWYRAPELLLGETSYNFKVDAWSVGCIMLEMRLGRPPYRGNDEVSQIKLIFEELGVPQDKYKWSDLLDVGVYSKDKDAQEIITERYRSLFDEEELKVLSGFLCLTSRRRLSVVNSRHFSIISAYKDTYIPLNFEESHELYMKERKETRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKP4</accession>
    <name>I6ZKP4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_100670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the methyltransferase superfamily. RRP8 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265380.1">
        <property type="nucleotide sequence ID" value="XM_009267105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKP4"/>
    <dbReference type="GeneID" id="20564497"/>
    <dbReference type="KEGG" id="ero:EROM_100670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100670"/>
    <dbReference type="HOGENOM" id="CLU_027694_1_2_1"/>
    <dbReference type="OrthoDB" id="10258825at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2150">
        <property type="entry name" value="Ribosomal RNA-processing protein 8, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007823">
        <property type="entry name" value="RRP8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042036">
        <property type="entry name" value="RRP8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="210" mass="24608" checksum="1E64F2017BB8FCAF" modified="2012-10-03" version="1">MSLEKKLMKRLEGGKFRMLNDRMYHGKGLKKKDLRLYHELYDLQVKRWPVNPLDIIIEKIKEKGGDMVIADVGCGDARIAKEFENVISLDLHPSKKGIVRCDMSKRIPLEDKSVDIAVCCLSMMIENIAVPTKEINRILKENGYWYVAEVRSRIPGVRLLERKFEAFGFEIKQVDVSNEQFVIFVLKKVLSKNWKKLPQIKLMPWLYKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKP7</accession>
    <name>I6ZKP7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">U1 snRNP protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Luc7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265385.1">
        <property type="nucleotide sequence ID" value="XM_009267110.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKP7"/>
    <dbReference type="GeneID" id="20564502"/>
    <dbReference type="KEGG" id="ero:EROM_100720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100720"/>
    <dbReference type="HOGENOM" id="CLU_030397_0_0_1"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004882">
        <property type="entry name" value="Luc7-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12375">
        <property type="entry name" value="RNA-BINDING PROTEIN LUC7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03194">
        <property type="entry name" value="LUC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="237" mass="27874" checksum="633488B2EC7665F7" modified="2012-10-03" version="1">MTDRAREVLDMLLGPDRDIYDPGKSFSAKKDVCIYMLVSFCPFELFRNTRKSIGKCKYNNHEEYYKAEYNRGGKERYEEYEWEFLRLLVEIALRVQDEIREQNSENMVDVSLLGKIQEKEEVFNKMYQDIERLGMEGNVEEAWHSFNECEKVREELERIKEAYYVKSNGVGMEKCTVCGVNLVLSDTDAKISRHLNGRLHRGHLRVRNKLGELLEKFGISSVTEIFPEGFSFKHLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKW3</accession>
    <name>I6ZKW3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265455.1">
        <property type="nucleotide sequence ID" value="XM_009267180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKW3"/>
    <dbReference type="GeneID" id="20564573"/>
    <dbReference type="KEGG" id="ero:EROM_101430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101430"/>
    <dbReference type="HOGENOM" id="CLU_162190_0_0_1"/>
    <dbReference type="OrthoDB" id="2189368at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031503">
        <property type="entry name" value="DUF5099"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17025">
        <property type="entry name" value="DUF5099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="110" mass="12821" checksum="75E355A2A6796C33" modified="2012-10-03" version="1">MESLVLWDGRYIGRLKKVPKTMLIIDRYGTISEKEKKGIKDSVMEVDIDFEEKTTHYSLVILCNTALRFNLINPLTLAECEIWFTRRVFSSRVFADALTHYSECEIRNGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL19</accession>
    <name>I6ZL19_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BolA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265515.1">
        <property type="nucleotide sequence ID" value="XM_009267240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL19"/>
    <dbReference type="GeneID" id="20564635"/>
    <dbReference type="KEGG" id="ero:EROM_110360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110360"/>
    <dbReference type="HOGENOM" id="CLU_109462_2_1_1"/>
    <dbReference type="OrthoDB" id="411584at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF3">
        <property type="entry name" value="SUFE-LIKE PROTEIN 1, CHLOROPLASTIC_MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="93" mass="10745" checksum="7C7CC2CC76D3F62E" modified="2012-10-03" version="1">MKGGLEAKMFNALKNEFCPTRLEVKNTSCDHTGHRIIEQEEGLETHFHVRVRSAMFNGMKFTDRHRLVYKTLEFAFSLGLHAIEIDCDSDSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL72</accession>
    <name>I6ZL72_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265570.1">
        <property type="nucleotide sequence ID" value="XM_009267295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL72"/>
    <dbReference type="GeneID" id="20564690"/>
    <dbReference type="KEGG" id="ero:EROM_110910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110910"/>
    <dbReference type="HOGENOM" id="CLU_1885742_0_0_1"/>
    <dbReference type="OrthoDB" id="2191452at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="142" mass="17042" checksum="14F869EF9A064F41" modified="2012-10-03" version="1">MKDLLQRFSYEYVRFQYTPCQDSVSKIYTLLQYAPDSLVVHRELEIIPSFELLRKCRDRVKVVVGEDPFQMVLRHLDGFNTFIFLEYEFNSFEHEFLRQASIVNKKSIFLFSEVQKDCMSGKESYRLVKLDLCLPWFAYKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLA2</accession>
    <name>I6ZLA2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111270</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265605.1">
        <property type="nucleotide sequence ID" value="XM_009267330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLA2"/>
    <dbReference type="GeneID" id="20564726"/>
    <dbReference type="KEGG" id="ero:EROM_111270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111270"/>
    <dbReference type="HOGENOM" id="CLU_080796_0_0_1"/>
    <dbReference type="OrthoDB" id="10255148at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="204" mass="23919" checksum="DFB8E7B4313FECA7" modified="2012-10-03" version="1">MGAINYLREIHRRKQSDVMRYLLRIRVCEYRQRGECFRVEKPTFLERARTLGYRAKQGYVLYIARVKKGNMKRNYHNGNTRGKCVNAGINQIKPSLRKQASAEMVAGKKCPNLGVLNSYWVGQDAAYKYYEVIMVDRHHPAIRNDSKINWICKNTMKHRECRGLTSASRKSRGLGKGVRFNKSKGGSRRSCWRRRNTLVLQKYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLH6</accession>
    <name>I6ZLH6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Caseine kinase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265665.1">
        <property type="nucleotide sequence ID" value="XM_009267390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLH6"/>
    <dbReference type="SMR" id="I6ZLH6"/>
    <dbReference type="GeneID" id="20564786"/>
    <dbReference type="KEGG" id="ero:EROM_111870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111870"/>
    <dbReference type="HOGENOM" id="CLU_019279_2_0_1"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="8"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="318" mass="37232" checksum="4BDDC7F94CEC5850" modified="2012-10-03" version="1">MRGEIMGYTICEEIGKGGFGKVYKVRTKEDQKAYALKLEDNKEKGRRNVIINEIQAYNELQGCDEIPLLVDHGFHRALAFLVLPLFRYSLKDILERHPRFFTRRSATIVAKKLLGTIEFIHKKGRLYRDLKPENVMFDYNNKVYLIDFGMSMPYLRKDGSHIPEIRGKNVCGTLWYMSINTHRGVEQSRRDDLESLFYLLILLYKSGLPWIEVGASMSKRQKARTRAIKEGLSVYELCDGISGKEYLIKFFQYVRNLKFSEKPDYRYLNSLLDEVLYSEKEFQGYKEMSSEDGKRNTKMGSISLWSKFISILNPFVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLI0</accession>
    <name>I6ZLI0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ste12 transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_111920</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STE12 transcription factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265670.1">
        <property type="nucleotide sequence ID" value="XM_009267395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLI0"/>
    <dbReference type="GeneID" id="20564791"/>
    <dbReference type="KEGG" id="ero:EROM_111920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111920"/>
    <dbReference type="HOGENOM" id="CLU_065182_0_0_1"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990526">
        <property type="term" value="C:Ste12p-Dig1p-Dig2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990527">
        <property type="term" value="C:Tec1p-Ste12p-Dig1p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003120">
        <property type="entry name" value="Ste12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052127">
        <property type="entry name" value="STE12_transcription_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47427:SF1">
        <property type="entry name" value="PROTEIN STE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02200">
        <property type="entry name" value="STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00424">
        <property type="entry name" value="STE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="374" mass="43141" checksum="FAB34CA9DDEEA709" modified="2012-10-03" version="1">MNAQSDKELFEFLANAPKEFRDGERIKKFQLKNGDYIHCVLWNMHFFITGTDIVKILVWRFQNAGRCLTSLKKFEEGVFSDLRNLKPGIDATLEGPRSEFLEFLYKNGCIRTQKKQKVFYWYSVPHDALFCDALERDLRRETNLFTYNKYINNLTRNSMLPHGMVGFVSHTRQNSKRIGVEVDPRYLRGIENKVLPMGMMYSQGRPIPQQGILFDQQHHKKAPTVPPQQKSPTIYDKQFEEIQKNGFLDQTFPGFNIKDKLFSSGDQQLDYDLHRTDGLFCNDLFSTAIIEKPSPNPDIGSSKKSKIYNTTPSSFLEELPDGFSLDDFDFLKDRAEKINADDESRQGSLKEGTNQRIPHDGVTLIPNAKGSLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZRY8</accession>
    <name>I6ZRY8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZRY8"/>
    <dbReference type="GeneID" id="20520627"/>
    <dbReference type="KEGG" id="ero:EROM_010010"/>
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    <dbReference type="OrthoDB" id="10471613at2759"/>
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        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="245" mass="28222" checksum="7A990DD3F8947325" modified="2012-10-03" version="1">MKDEKSDHADDDHRMMASAIYVASMIITTILWFLRIEINGNRVLAVLEVLVELSFTWFISIYWVKRIWGKNLKEPKGKVSRLLCWVAFYFSLVYAFLLVPSFIFMIVTKKVRNLGKFLGEDSPCIALVYIIINALGFLPRPSSISYKIDLLAYVCIQVIFVVSMIVTYKKNREDILPYTSMACGLGLGAFTLLKELSVIPREDCSEKNSKNYNRIRYLVAITSIIVLYEFKIKLENTPPHIARLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS77</accession>
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        </submittedName>
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    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7 8">SJ-2008</strain>
        </source>
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    <dbReference type="EMBL" id="CP003518">
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    <dbReference type="KEGG" id="ero:EROM_011120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011120"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847:SF637">
        <property type="entry name" value="WD REPEAT DOMAIN 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22847">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
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        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="10"/>
            <end position="51"/>
        </location>
    </feature>
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        <location>
            <begin position="52"/>
            <end position="93"/>
        </location>
    </feature>
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        <location>
            <begin position="94"/>
            <end position="135"/>
        </location>
    </feature>
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        <location>
            <begin position="136"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="190"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="6">
        <location>
            <begin position="577"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="domain" description="Coatomer alpha subunit C-terminal" evidence="5">
        <location>
            <begin position="789"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="701"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="701"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="971" mass="109794" checksum="4D6065E6F7E9A72E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSZ4</accession>
    <name>I6ZSZ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcription initiation factor TFIIA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_030950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264213.1">
        <property type="nucleotide sequence ID" value="XM_009265938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSZ4"/>
    <dbReference type="GeneID" id="20521006"/>
    <dbReference type="KEGG" id="ero:EROM_030950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030950"/>
    <dbReference type="HOGENOM" id="CLU_030027_2_1_1"/>
    <dbReference type="OrthoDB" id="6275927at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07976">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004855">
        <property type="entry name" value="TFIIA_asu/bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12694:SF8">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03153">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="157" mass="17747" checksum="FEECAC3D80580139" modified="2012-10-03" version="1">MLKIYEDIISEVISKLEQAGDEINMGKSAIHELRNEWRERLIEYTQGEWGGERNGCNDGEEGYRGYERPSEGRVNGFNGKTTVEKDVGMFEDYESLSASSGDSEIIGKKDNVGNCMVCLYVKVNMSKGKWKCTFKQGFISIGNIDFVFNSAQGELEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTE6</accession>
    <name>I6ZTE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264423.1">
        <property type="nucleotide sequence ID" value="XM_009266148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTE6"/>
    <dbReference type="GeneID" id="20521225"/>
    <dbReference type="KEGG" id="ero:EROM_041620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041620"/>
    <dbReference type="HOGENOM" id="CLU_1496650_0_0_1"/>
    <dbReference type="OrthoDB" id="2158148at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="194" mass="22967" checksum="25CBCA47406C151C" modified="2012-10-03" version="1">MGLCLEKIEKSISYMDDTYDANFGEWIRNEDNARIVAYNMKKYVDNYKTSDFIIVVKWIVKDWTLKSIIIFSKKMLVEDIKVLSFRKSEEDKDRYNKRIKIISGLIFTWNPVFITEFIVSITRSFGTNEKCKLLINLLEVFEARKLSEILSQLEAKIEQKTWNELFKTFNDEASKKSRPRSKRTASILRAYNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTG0</accession>
    <name>I6ZTG0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Aminoacid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_050130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264443.1">
        <property type="nucleotide sequence ID" value="XM_009266168.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTG0"/>
    <dbReference type="GeneID" id="20521245"/>
    <dbReference type="KEGG" id="ero:EROM_050130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050130"/>
    <dbReference type="HOGENOM" id="CLU_049198_0_0_1"/>
    <dbReference type="OrthoDB" id="2192968at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF678">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="379"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="8"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="408" mass="45019" checksum="B0DE68328D2BB190" modified="2012-10-03" version="1">MSKVLTEYSATVTMITSMMGASIYFTPVAFRFVGYIYGWIIIFIVALLTSFSLYCISYSAMNMPVGPTYSSLGACISPKLKNVLDAFVIVNSLTANICLYRYLSELIVAAFPVLLLVFPSYETARKGVVVVLLIPLLTLSFHKNLSSLRYVSLAMVVSIVYFILLLISYNLLFYPGRSNYETTPFGNGFFFSVPIFITAMACQSNMVKVLDEMASYSVRKVKFVSIWSGVGGGLIYGSIGHLGYSLFGNGLDGDLLGIFADKNSVINAQLAGTIDRYLVSSKIAVYGSMLIFIGSFPMQLNPLITTLFNIFPERRRTENIRLILMVFLMILCFSLALIRDLSAGLVISIAGATVTNFISFLFPFVFFISAKKKFSTSTFLALVSMVACISSSIYMVFNILGKTTDRSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTR6</accession>
    <name>I6ZTR6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264563.1">
        <property type="nucleotide sequence ID" value="XM_009266288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTR6"/>
    <dbReference type="GeneID" id="20521367"/>
    <dbReference type="KEGG" id="ero:EROM_051360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051360"/>
    <dbReference type="HOGENOM" id="CLU_1602703_0_0_1"/>
    <dbReference type="OrthoDB" id="2191842at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="166" mass="19131" checksum="C54D60211C0DDDC7" modified="2012-10-03" version="1">MGVRIITYSSSISLPPTMFIRNTLLQNIQMKLSYTHIHFTDSLEDISTCDILVVDGNIMSVDTLEDLKKIHSKTILWTKDMVNDTIWLVEKFDKASDKNYIAIINGHVNVEERDAESHAPQKPSLGPKTESKEDPKRSYLDLKFSNLEKRRPIESIFSRLHLSRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU38</accession>
    <name>I6ZU38_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5094 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264653.1">
        <property type="nucleotide sequence ID" value="XM_009266378.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU38"/>
    <dbReference type="GeneID" id="20521459"/>
    <dbReference type="KEGG" id="ero:EROM_060620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060620"/>
    <dbReference type="HOGENOM" id="CLU_133365_0_0_1"/>
    <dbReference type="OrthoDB" id="2187979at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031519">
        <property type="entry name" value="DUF5094"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17015">
        <property type="entry name" value="DUF5094"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5094" evidence="2">
        <location>
            <begin position="5"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="20056" checksum="EFC7796A7FA6CA8E" modified="2012-10-03" version="1">MRTPRKKITFKTPRKGTKTPVKDGHASDTKENKTPKIENKDEQKGLDSASRYKQILIEMKMKEEDLIESYKKENEYLRSLKTDALLYKKFVGFDIVESNGCYEVTHEIVSNNITKFIRFVLTPEDSSYIYKLVESRNIELPDFLSEEITFDECQIKTFFFKVMEAVITKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU41</accession>
    <name>I6ZU41_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Secreted/periplasmic Zn-dependent insulinase-like peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_060680</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264658.1">
        <property type="nucleotide sequence ID" value="XM_009266383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU41"/>
    <dbReference type="GeneID" id="20521464"/>
    <dbReference type="KEGG" id="ero:EROM_060680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060680"/>
    <dbReference type="HOGENOM" id="CLU_004639_1_1_1"/>
    <dbReference type="OrthoDB" id="952271at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000012">
        <property type="entry name" value="Protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054734">
        <property type="entry name" value="PqqF-like_C_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22456">
        <property type="entry name" value="PqqF-like_C_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="9">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="10">
        <location>
            <begin position="184"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="11">
        <location>
            <begin position="372"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme PQQ synthesis protein F-like C-terminal lobe" evidence="12">
        <location>
            <begin position="732"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="874" mass="101687" checksum="71379AF26BEC3C97" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUH2</accession>
    <name>I6ZUH2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264828.1">
        <property type="nucleotide sequence ID" value="XM_009266553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUH2"/>
    <dbReference type="GeneID" id="20521639"/>
    <dbReference type="KEGG" id="ero:EROM_070800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070800"/>
    <dbReference type="HOGENOM" id="CLU_2210008_0_0_1"/>
    <dbReference type="OrthoDB" id="2193922at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="109" mass="12688" checksum="A8370F118ED0AF5B" modified="2012-10-03" version="1">MSRIKNGKEGEEAYTLTDECLEELLPIDFYDKSRKPRDEQKIEFMVGKEKSIFEGERRRDFVDIVSALEVENLKMEAEIGRLAEKEDRIDKENNALKDILYGILSKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUK6</accession>
    <name>I6ZUK6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264878.1">
        <property type="nucleotide sequence ID" value="XM_009266603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUK6"/>
    <dbReference type="GeneID" id="20521690"/>
    <dbReference type="KEGG" id="ero:EROM_071300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071300"/>
    <dbReference type="HOGENOM" id="CLU_135798_0_0_1"/>
    <dbReference type="OrthoDB" id="2191285at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="161" mass="19239" checksum="299F9007AD6E0688" modified="2012-10-03" version="1">MGVKWKCPMERSEFLKMFEKTKTGMFVPKDQSQNWCRHFGMRKNKVLYLCEEEVLYLYDREVKEEYPVRVKAYFFIKNSCLNLLPAEGNRLLLYKRHRDFNRKKDKPICPMRYVSRDEYIEDASLGIEDEALCILSDDVFTFLKIKGIEKLDNGTPESLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUM2</accession>
    <name>I6ZUM2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">mRNA decapping enzyme 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_071550</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264903.1">
        <property type="nucleotide sequence ID" value="XM_009266628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUM2"/>
    <dbReference type="GeneID" id="20521719"/>
    <dbReference type="KEGG" id="ero:EROM_071550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071550"/>
    <dbReference type="HOGENOM" id="CLU_008108_5_0_1"/>
    <dbReference type="OrthoDB" id="18996at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="9">
        <location>
            <begin position="80"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="27728" checksum="80512EBA49B57B10" modified="2012-10-03" version="1">MIPEDILDSIASRFLVGLGNQERNAAERLFFAVEEAHWFLMDNYKISDLSFADFSRELLGHVGIKMNIEEAHKSFVRYRQSVKVYGAILVDPSISHVLAVKEKKKTKNYSFPKGKKCMDEDGTSCAIREVYEETGYDVQNKICNLPITIFDKITLYFVFNVKQSFPFEAQTIKEIEEIKWLSTRKLSRGEYRRGYSIVSAAFKKASYLLEAMRKSRFRFNTKKITQRIDKLLGKTKAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUY1</accession>
    <name>I6ZUY1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GATA binding factor 1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_081400</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265053.1">
        <property type="nucleotide sequence ID" value="XM_009266778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUY1"/>
    <dbReference type="GeneID" id="20521875"/>
    <dbReference type="KEGG" id="ero:EROM_081400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081400"/>
    <dbReference type="HOGENOM" id="CLU_1209821_0_0_1"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00202">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039355">
        <property type="entry name" value="Transcription_factor_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000679">
        <property type="entry name" value="Znf_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00619">
        <property type="entry name" value="GATAZNFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="229" mass="26426" checksum="A41DB66C0DF5C8F7" modified="2012-10-03" version="1">MLSKQGFCSNCNTTATPLWRRAEDGSYLCNACGLYYKIHGRKRPTNFKTDSSKPRVRCKRIGGEAGGAYDPEMPWTLYKHRSRTQGLFNLYQEENQMNSVSKHEGYPTKHDNLKGGREAPQINSSENILYEGFGMSEYGRNTILEDKRDYRRHTNVATKSLIRRHFDKRPGRIPPFMSEIESKETSNEKEPEKSTSEPWIKANEEHETDLEDSEVIAINALLDLSRERT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUZ0</accession>
    <name>I6ZUZ0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosome biogenesis regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in ribosomal large subunit assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RRS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265063.1">
        <property type="nucleotide sequence ID" value="XM_009266788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUZ0"/>
    <dbReference type="GeneID" id="20521885"/>
    <dbReference type="KEGG" id="ero:EROM_081500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081500"/>
    <dbReference type="HOGENOM" id="CLU_1731447_0_0_1"/>
    <dbReference type="OrthoDB" id="28455at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007023">
        <property type="entry name" value="Ribosom_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04939">
        <property type="entry name" value="RRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="151" mass="17257" checksum="E6CC5A61B3EBDEEC" modified="2012-10-03" version="1">MNHLKLLTVISNNAVGRSDINQETSNLLKGIVKEIKACQSSRRGSDLVFALPEPTMILPRQHLKEHTETRWERFARSKGIKKKKKSQIVYDKEIGDYVPRYGPHSKKNRILKAAVKEGEVTLSALRRQKKNNIEKNEANRLANISRKKGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVW2</accession>
    <name>I6ZVW2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="2 6">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_100800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + [thioredoxin]-disulfide + H2O = a ribonucleoside 5'-diphosphate + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265393.1">
        <property type="nucleotide sequence ID" value="XM_009267118.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVW2"/>
    <dbReference type="GeneID" id="20564510"/>
    <dbReference type="KEGG" id="ero:EROM_100800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100800"/>
    <dbReference type="HOGENOM" id="CLU_000404_1_0_1"/>
    <dbReference type="OrthoDB" id="3000483at2759"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005971">
        <property type="term" value="C:ribonucleoside-diphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009263">
        <property type="term" value="P:deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01679">
        <property type="entry name" value="RNR_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573:SF6">
        <property type="entry name" value="RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="R1 subunit of ribonucleotide reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0215">Deoxyribonucleotide synthesis</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonucleotide reductase large subunit N-terminal" evidence="7">
        <location>
            <begin position="137"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Ribonucleotide reductase large subunit C-terminal" evidence="8">
        <location>
            <begin position="211"/>
            <end position="731"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="769" mass="87965" checksum="4B341DE250F63D62" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACW2</accession>
    <name>I7ACW2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ubiquitin conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_010810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263922.1">
        <property type="nucleotide sequence ID" value="XM_009265647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACW2"/>
    <dbReference type="GeneID" id="20520708"/>
    <dbReference type="KEGG" id="ero:EROM_010810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010810"/>
    <dbReference type="HOGENOM" id="CLU_030988_10_1_1"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="3">
        <location>
            <begin position="5"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="191" mass="21793" checksum="40611A02A7F52773" modified="2012-10-03" version="1">MLSPGARRRLSKEEDMLKDSTGDERKLFRACPRGPKENRNYKVWDIYFTLGGDSLYAGRILKAVMEFPDCYPLQPPTLKFVSKMFHPNIYEDGKMCISILEEDAPDPTGYGDSKDKWAPVQNIRTILLSIIVILNAPNTSSPANVDASVMHRDNHEEYAKKVIKIAREEDEKLRSTDPQAREIALEMESED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD80</accession>
    <name>I7AD80_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc finger PHD-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_020800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264052.1">
        <property type="nucleotide sequence ID" value="XM_009265777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD80"/>
    <dbReference type="GeneID" id="20520840"/>
    <dbReference type="KEGG" id="ero:EROM_020800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020800"/>
    <dbReference type="HOGENOM" id="CLU_1816373_0_0_1"/>
    <dbReference type="OrthoDB" id="1928087at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger PHD-type" evidence="4">
        <location>
            <begin position="10"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="142" mass="16408" checksum="AEA47E441EEB63A8" modified="2012-10-03" version="1">MYLGIRGYIRCHCRLIGRDPHMIHCSSCGNWLHTVCCGFFSNEDKRISKETFSCFYCLGSITKADNANALFRRVLSIIYTEDLRSKAWLSSRLGITEWQSTKQTRRLANEGFVKVIGKHRAISYVVIKTQETKDKVKKYFGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD94</accession>
    <name>I7AD94_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gluconate transport-inducing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020950</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264067.1">
        <property type="nucleotide sequence ID" value="XM_009265792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD94"/>
    <dbReference type="GeneID" id="20520856"/>
    <dbReference type="KEGG" id="ero:EROM_020950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020950"/>
    <dbReference type="HOGENOM" id="CLU_1562863_0_0_1"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="20040" checksum="4B8078B53973E2CC" modified="2012-10-03" version="1">MASIESFYGSVENESDCLVLIDLARRGLIPRIQRRLTSKERKQIRHGSIFIYCEEESGIRRWTDGMSWTPSRVQGVFLMYKQLLSGNPMIKRTYSATCGDKNFHIVGYIQVYSSNDELPPVSALYKGVDFPTDIRIKKKLNFQQERCCRREVKSIESVEPNEDFQFDVYWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADB4</accession>
    <name>I7ADB4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chromosome condensation complex Condensin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_021150</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND1 (condensin subunit 1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264087.1">
        <property type="nucleotide sequence ID" value="XM_009265812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADB4"/>
    <dbReference type="GeneID" id="20520877"/>
    <dbReference type="KEGG" id="ero:EROM_021150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021150"/>
    <dbReference type="HOGENOM" id="CLU_319599_0_0_1"/>
    <dbReference type="OrthoDB" id="436262at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000779">
        <property type="term" value="C:condensed chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010032">
        <property type="term" value="P:meiotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026971">
        <property type="entry name" value="CND1/NCAPD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032682">
        <property type="entry name" value="Cnd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007673">
        <property type="entry name" value="Condensin_cplx_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222">
        <property type="entry name" value="CONDENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14222:SF2">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12717">
        <property type="entry name" value="Cnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017127">
        <property type="entry name" value="Condensin_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Condensin complex subunit 1 C-terminal" evidence="11">
        <location>
            <begin position="753"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="917" mass="105736" checksum="1E18480EE6E1F31D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADJ9</accession>
    <name>I7ADJ9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SKI-interacting protein SKIP SNW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264132.1">
        <property type="nucleotide sequence ID" value="XM_009265857.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADJ9"/>
    <dbReference type="GeneID" id="20520923"/>
    <dbReference type="KEGG" id="ero:EROM_030160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030160"/>
    <dbReference type="HOGENOM" id="CLU_1927572_0_0_1"/>
    <dbReference type="OrthoDB" id="6513151at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004015">
        <property type="entry name" value="SKI-int_prot_SKIP_SNW-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02731">
        <property type="entry name" value="SKIP_SNW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SKI-interacting protein SKIP SNW" evidence="2">
        <location>
            <begin position="20"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="135" mass="15612" checksum="F239FDF588566453" modified="2012-10-03" version="1">MGVKEIEEGRYGRVDIVSPDPLKITCKRGTKKHRKEGVGDKDKPRKLYKNKSKVKLVKCVSMWKNPTSQIIPLEMRVAHDRFRSIQPEINLGRFGELKSSLDSAEGLYSQETKKKAIKKERIEETKRTDKSQLQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADK4</accession>
    <name>I7ADK4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Vacuolar ATP synthase subunit F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_030205</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264137.1">
        <property type="nucleotide sequence ID" value="XM_009265862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADK4"/>
    <dbReference type="GeneID" id="20520928"/>
    <dbReference type="KEGG" id="ero:EROM_030205"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030205"/>
    <dbReference type="HOGENOM" id="CLU_135754_0_1_1"/>
    <dbReference type="OrthoDB" id="10261947at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861:SF2">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13861">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="95" mass="10477" checksum="E2F5D197CE5451E8" modified="2012-10-03" version="1">MDERTRIGIIGDEETLTGFLIAGVESVHDNPNLVQVTPNTAEDDLKRIFCSLTGRKDLAIILVCDFAAEKLKEEIDAYNEVVPAVLVIASKNKYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADX8</accession>
    <name>I7ADX8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264302.1">
        <property type="nucleotide sequence ID" value="XM_009266027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADX8"/>
    <dbReference type="GeneID" id="20521100"/>
    <dbReference type="KEGG" id="ero:EROM_040360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040360"/>
    <dbReference type="HOGENOM" id="CLU_363295_0_0_1"/>
    <dbReference type="OrthoDB" id="2193072at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="771" mass="89241" checksum="57F264C7B14D5273" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE67</accession>
    <name>I7AE67_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_041610</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SWD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264422.1">
        <property type="nucleotide sequence ID" value="XM_009266147.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE67"/>
    <dbReference type="GeneID" id="20521224"/>
    <dbReference type="KEGG" id="ero:EROM_041610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041610"/>
    <dbReference type="HOGENOM" id="CLU_943649_0_0_1"/>
    <dbReference type="OrthoDB" id="27537at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037867">
        <property type="entry name" value="Swd2/WDR82"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19861">
        <property type="entry name" value="WD40 REPEAT PROTEIN SWD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="32919" checksum="C2DC6259D3866D2F" modified="2012-10-03" version="1">MILTDEAFSEFKRSKPLGLSISPEEVEYSTDGLVLSSTQGNVLRLYSSISGNIQNIIHVPSIQKYKFMYPNAIIHSASNSLHLLSVFDNRYVSTFSGHKSQINALSVCPGEDTVLSSSYDSTNYWDTRKKNPVYRINAPNSISCLSLSNDYAILINGTLLKIYDKRNTKGPKSTSSLPEKRYKEIYYSPDGSFIIASEEKSHLFLSSDGTIRNSINLERGGSGCITPDSKFFLCCESSSILVYHLRTKRKIHAFKTPGFDNMKARFNPCYAQFVSSSNLLNIWAIKEHPEQSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AED1</accession>
    <name>I7AED1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Syntaxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264512.1">
        <property type="nucleotide sequence ID" value="XM_009266237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AED1"/>
    <dbReference type="GeneID" id="20521315"/>
    <dbReference type="KEGG" id="ero:EROM_050830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050830"/>
    <dbReference type="HOGENOM" id="CLU_090728_0_0_1"/>
    <dbReference type="OrthoDB" id="421009at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="148"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="240" mass="28166" checksum="813B9AEE95E6E271" modified="2012-10-03" version="1">MDRTDEFVKIIQTTLIPQQGKPLLSSPYIEAFEIDTMIERTLSEISRMLDKERVYESFTLQSKIDRASELVGKMRDVARLEMRCRNDQEAASYANLEGMIKNRVSRYSIKLKEFINKRNERRQAISERRKEFDSECSLEHQDTVLMEREVVTERIKERQKISMQISEIGQIMEEISMHVSLQEESFRRIDDLMATSDSLISGSLDLMKKTWANVSGTRPAIIKFIAFWVVLALAFWLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AET0</accession>
    <name>I7AET0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">histidine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060560</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264647.1">
        <property type="nucleotide sequence ID" value="XM_009266372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AET0"/>
    <dbReference type="GeneID" id="20521453"/>
    <dbReference type="KEGG" id="ero:EROM_060560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060560"/>
    <dbReference type="HOGENOM" id="CLU_025113_2_0_1"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476:SF7">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11476">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Class II Histidinyl-tRNA synthetase (HisRS)-like catalytic core" evidence="4">
        <location>
            <begin position="9"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="276"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="317" mass="36333" checksum="724CAC065053861E" modified="2012-10-03" version="1">MDSLKTPKGTTDESPRQCFLYEEIIEKIRGVFVLYGAVPISTPTFEMKSILTNKYGEDTKLIYDLKDQGGEICALRYDLTVPFARYLASNKIRKMKRYQIGRVYRRDQPSIVKGRLREFMQADFDIAGECIRMMADAEVISCVDRILKEFEIGEFKIKVNDRRILTSILEAVGVPCESYGTVCSTIDKIEKMSWKDISKEFIQKGLSEHQVNMIRKYMEMSGKENVLKLLRECPAYEIERCKEGVHDMEELFKFCRILGCEGSLVMDVSLARGLDYYTGMILEAEYIGKSVGSVIGGGRYDNLTENLGELQKTLHKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEV7</accession>
    <name>I7AEV7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060930</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264682.1">
        <property type="nucleotide sequence ID" value="XM_009266407.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEV7"/>
    <dbReference type="GeneID" id="20521489"/>
    <dbReference type="KEGG" id="ero:EROM_060930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060930"/>
    <dbReference type="HOGENOM" id="CLU_545152_0_0_1"/>
    <dbReference type="OrthoDB" id="2187767at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="500" mass="59226" checksum="E5DCDC7C4A03DCCB" modified="2012-10-03" version="1">MKGIKEYIKDRLGEYKIELDVDSVIEELTLSNKINEFMPPSSIYTVFLMHLGKNDEMYRSILNGEYLFDIEVGLNDEKSLYCDKELKDKIMKIYGERARYVYVKTSGSRHFIGIRLSNRGYDPAAGYSGPESTIPYFLLVKGLKEFRIDDFEWNEIIFGHRIMEDERSKYIEILEHIKRIRLPVQIIDSDAMHMATSITNVHECYLHCGSHANWPEDEDALDCAKTALYCLIYKKSKYRSAIGYSYVLLKYRCSYFKFKIMIRRDRRAEFRVNTRISEVIAQESDIFKKNIRFVKMFLDCHGYFPVYLDDRLVELICLMVGREISTFGRFFIEFLRYQVKLEGLTLNLETLKVTENKNKRFEVVYQHDIVVVRPPPLKIIQRLNGLKKAVVKQKIRLFDESFRLQTYKLLQPFFKDYDFVLSLSDKPGFIEVKDKVMQPFLFGVPLIDEFLLPNLKSKGYFFYSPRHSVLMVKVNEESNPEELLYVLLLKTGFRYFLRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF42</accession>
    <name>I7AF42_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA replication licensing factor Mcm7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_070440</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264792.1">
        <property type="nucleotide sequence ID" value="XM_009266517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF42"/>
    <dbReference type="GeneID" id="20521603"/>
    <dbReference type="KEGG" id="ero:EROM_070440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070440"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF48">
        <property type="entry name" value="DNA HELICASE MCM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="5">
        <location>
            <begin position="222"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="556" mass="61547" checksum="1673034AF44423D1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFI9</accession>
    <name>I7AFI9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264987.1">
        <property type="nucleotide sequence ID" value="XM_009266712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFI9"/>
    <dbReference type="GeneID" id="20521807"/>
    <dbReference type="KEGG" id="ero:EROM_080710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080710"/>
    <dbReference type="HOGENOM" id="CLU_1360387_0_0_1"/>
    <dbReference type="OrthoDB" id="2193787at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="202" mass="22936" checksum="9925C714FFBC4259" modified="2012-10-03" version="1">MDEIFIGSPSKREIEMYLLNKEYSLLNNIKLRDDILSKRGMGGSSENEAELKFEFLKGGKPWENGLVQVVDGSSEKRKASIGYQIKNGRKTSGEKDFNGKIEKKRIGTKKIIERNTIKNSGYRYVTLVFVGSNEFPKPVTKRHRKKVRFCDSACPGAHNTGKPSSILLPASKTRKLPRTRKKEKVFVESLALDTENESEEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG07</accession>
    <name>I7AG07_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_090380</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ESF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265152.1">
        <property type="nucleotide sequence ID" value="XM_009266877.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG07"/>
    <dbReference type="GeneID" id="20521976"/>
    <dbReference type="KEGG" id="ero:EROM_090380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090380"/>
    <dbReference type="HOGENOM" id="CLU_1005088_0_0_1"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012580">
        <property type="entry name" value="NUC153"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056750">
        <property type="entry name" value="RRM_ESF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08159">
        <property type="entry name" value="NUC153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25121">
        <property type="entry name" value="RRM_ESF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ESF1 RRM" evidence="7">
        <location>
            <begin position="94"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="NUC153" evidence="6">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="209"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="308" mass="35519" checksum="C45AA00ACC00858D" modified="2012-10-03" version="1">MTIFRIQKHRWMKEAKGSEEGNVAAIGGATKRLACLGMNWANLDIKDLFKIFDSALPYSSPLNVKLCKTKIGKEKLGDNNIFVKSCKKGSIRECYVAVAEFEDIEDSKHVYSTYDGAELGCSGMIFDLRFVPDSLDLQNVCDEVFSDDECVEKDFRTKERDEEESSGMESELQRLFKEKEINFDLVSKLIDISDDENIPSLQNELVVSSENERDDFLESDSDDFNNKPSKNKKVKEKKDKKEDIEPIPRIKPTDECDGFVFDPKDKRFEAIFEDDDFTIDPTHPEYKRKSGLKEILDEKRRRLKDNID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG25</accession>
    <name>I7AG25_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA repair protein Mms21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_090680</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NSE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265182.1">
        <property type="nucleotide sequence ID" value="XM_009266907.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG25"/>
    <dbReference type="GeneID" id="20564292"/>
    <dbReference type="KEGG" id="ero:EROM_090680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090680"/>
    <dbReference type="HOGENOM" id="CLU_1865721_0_0_1"/>
    <dbReference type="OrthoDB" id="2190619at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061665">
        <property type="term" value="F:SUMO ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026846">
        <property type="entry name" value="Nse2(Mms21)"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004181">
        <property type="entry name" value="Znf_MIZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21330:SF1">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE NSE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11789">
        <property type="entry name" value="zf-Nse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SP-RING-type" evidence="10">
        <location>
            <begin position="82"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="136" mass="16032" checksum="61D3E6CFF6B4E95C" modified="2012-10-03" version="1">MEEHILRREEVLRKAKSMIMETDDPSEELLRKILIHQELLSNRNIILNDDFYSILNSKISIHPEMIELTNKKEQVECVRMERKLICPISQKEVVDAYIGDCGHVLEKKEAMKYIRNNSRAICPQIGCNKILVKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGC3</accession>
    <name>I7AGC3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265307.1">
        <property type="nucleotide sequence ID" value="XM_009267032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGC3"/>
    <dbReference type="GeneID" id="20564420"/>
    <dbReference type="KEGG" id="ero:EROM_091960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091960"/>
    <dbReference type="HOGENOM" id="CLU_2291684_0_0_1"/>
    <dbReference type="OrthoDB" id="2191009at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="101" mass="11647" checksum="50E85F3C4C3961A3" modified="2012-10-03" version="1">MGANEEFISPLDLAKRLRSNTHNPSETLLSIQCLSKPLSINRGQVREIRRLSRFLFHLSVDKDVLVSLVFNSEAIDVFMDFSKITVHRVYDGIYYSVDIEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGE9</accession>
    <name>I7AGE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rho1-like RAS GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_100230</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265337.1">
        <property type="nucleotide sequence ID" value="XM_009267062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGE9"/>
    <dbReference type="GeneID" id="20564453"/>
    <dbReference type="KEGG" id="ero:EROM_100230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100230"/>
    <dbReference type="HOGENOM" id="CLU_041217_21_2_1"/>
    <dbReference type="OrthoDB" id="8830751at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00157">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="203" mass="23127" checksum="00CB61D508C84281" modified="2012-10-03" version="1">MNSKTHKSGKVVIIGDGACGKTCLLEVFRRNQFPETYIPTVVDNFVKEVKIDDDKYVSLALWDTAGQEDYDTIRPLSYKETDLVLLCYTIENKKKIPNISRKWLMEIKNYCPSSQFFLIGLKKDVRDMEDPTVDKSSMVSESEGRKIADNINAAKFFECSSRTRENVNLVFLEAAKHIWNTKGAASESGPCSFFSCMSCCHYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGN8</accession>
    <name>I7AGN8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Lysosomal dipeptide transporter MFSD1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Major facilitator superfamily domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="ordered locus">EROM_101300</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26 27">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="23">Lysosomal dipeptide uniporter that selectively exports lysine, arginine or histidine-containing dipeptides with a net positive charge from the lysosome lumen into the cytosol. Could play a role in a specific type of protein O-glycosylation indirectly regulating macrophages migration and tissue invasion. Also essential for liver homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-alanyl-L-lysine(out) = L-alanyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79415"/>
            <dbReference type="ChEBI" id="CHEBI:192470"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-alpha-aminoacyl-L-arginine(out) = L-alpha-aminoacyl-L-arginine(in)</text>
            <dbReference type="Rhea" id="RHEA:79367"/>
            <dbReference type="ChEBI" id="CHEBI:229968"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-alpha-aminoacyl-L-histidine(out) = L-alpha-aminoacyl-L-histidine(in)</text>
            <dbReference type="Rhea" id="RHEA:79375"/>
            <dbReference type="ChEBI" id="CHEBI:229967"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alpha-aminoacyl-L-lysine(out) = L-alpha-aminoacyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79383"/>
            <dbReference type="ChEBI" id="CHEBI:229966"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-arginyl-L-alpha-amino acid(out) = L-arginyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79371"/>
            <dbReference type="ChEBI" id="CHEBI:84315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-arginyl-glycine(out) = L-arginyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79391"/>
            <dbReference type="ChEBI" id="CHEBI:229955"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-aspartyl-L-lysine(out) = L-aspartyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79411"/>
            <dbReference type="ChEBI" id="CHEBI:229953"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-histidyl-L-alpha-amino acid(out) = L-histidyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79379"/>
            <dbReference type="ChEBI" id="CHEBI:229964"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-histidyl-glycine(out) = L-histidyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79395"/>
            <dbReference type="ChEBI" id="CHEBI:229957"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-L-alanine(out) = L-lysyl-L-alanine(in)</text>
            <dbReference type="Rhea" id="RHEA:79399"/>
            <dbReference type="ChEBI" id="CHEBI:229954"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-lysyl-L-alpha-amino acid(out) = L-lysyl-L-alpha-amino acid(in)</text>
            <dbReference type="Rhea" id="RHEA:79387"/>
            <dbReference type="ChEBI" id="CHEBI:229965"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl-L-lysine(out) = L-lysyl-L-lysine(in)</text>
            <dbReference type="Rhea" id="RHEA:79403"/>
            <dbReference type="ChEBI" id="CHEBI:229956"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl-glycine(out) = L-lysyl-glycine(in)</text>
            <dbReference type="Rhea" id="RHEA:79407"/>
            <dbReference type="ChEBI" id="CHEBI:191202"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Homodimer. Interacts with lysosomal protein GLMP (via lumenal domain); the interaction starts while both proteins are still in the endoplasmic reticulum and is required for stabilization of MFSD1 in lysosomes but has no direct effect on its targeting to lysosomes or transporter activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265442.1">
        <property type="nucleotide sequence ID" value="XM_009267167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGN8"/>
    <dbReference type="GeneID" id="20564560"/>
    <dbReference type="KEGG" id="ero:EROM_101300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101300"/>
    <dbReference type="HOGENOM" id="CLU_027449_0_0_1"/>
    <dbReference type="OrthoDB" id="424834at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052187">
        <property type="entry name" value="MFSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23512:SF3">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="445"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="479"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00044900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00044924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00044985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="EMBL" id="AFN83945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="515" mass="57696" checksum="DBDA64909CD70749" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGU0</accession>
    <name>I7AGU0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sel1 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sel-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265507.1">
        <property type="nucleotide sequence ID" value="XM_009267232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGU0"/>
    <dbReference type="GeneID" id="20564627"/>
    <dbReference type="KEGG" id="ero:EROM_110280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110280"/>
    <dbReference type="HOGENOM" id="CLU_000288_36_14_1"/>
    <dbReference type="OrthoDB" id="272077at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050767">
        <property type="entry name" value="Sel1_AlgK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102:SF160">
        <property type="entry name" value="ERAD-ASSOCIATED E3 UBIQUITIN-PROTEIN LIGASE COMPONENT HRD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11102">
        <property type="entry name" value="SEL-1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08238">
        <property type="entry name" value="Sel1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81901">
        <property type="entry name" value="HCP-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="588" mass="66279" checksum="B11510719EB7E1B4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALF0</accession>
    <name>I7ALF0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">P-ATPase-V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_020240</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263996.1">
        <property type="nucleotide sequence ID" value="XM_009265721.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALF0"/>
    <dbReference type="GeneID" id="20520782"/>
    <dbReference type="KEGG" id="ero:EROM_020240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020240"/>
    <dbReference type="HOGENOM" id="CLU_001828_4_1_1"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="798"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="836"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="876"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="910"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="948"/>
            <end position="971"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="12">
        <location>
            <begin position="226"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="973" mass="109808" checksum="57B91C72D81B0ECB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALH2</accession>
    <name>I7ALH2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020490</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264021.1">
        <property type="nucleotide sequence ID" value="XM_009265746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALH2"/>
    <dbReference type="GeneID" id="20520809"/>
    <dbReference type="KEGG" id="ero:EROM_020490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020490"/>
    <dbReference type="HOGENOM" id="CLU_523689_0_0_1"/>
    <dbReference type="OrthoDB" id="2194974at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031494">
        <property type="entry name" value="Beta_lactamase3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17030">
        <property type="entry name" value="Beta_lactamase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="470" mass="54332" checksum="E0BA44CB99E15028" modified="2012-10-03" version="1">MVLIKRISSGSKSCYILQASEQTFLLDYPSRIYGMHFSFPSFNKGPKPLKLILPKFVDDIDHILVSRSDSLGVLFLEKDIPIYITEPVFEQILIKYQNLLRLSIHYEDEEEHGISIRDANIERFKGNARFIKFNEKCQFDDVLITPKPAGTFIGWTNYLIELNNRRTIYYLSSLANKLRLSQESGSISSDYLIINTDRPPSSHNIEEFSDYIGDLRFDVAIIPLEITTILTEVLLHTLFVVQEKGVTPIYIVSSEFNRFHTIISIENEWLSKPFSSVKDPFPIKSYRSLHVVDTIDEISEVAGPAIVFCDPLEFSLYQHPIFDGQKTISINGVVPLADAHYNLKLELDFDEISSIHTGTIIYNPDPDEYLYIESASNHHYLIVNGSDVQVHNSEIYLNGKLKFSDKYGFRKKMTFNQKRCKLSDLFTNDTFLYNEGSYYFPRKAIKVTIENNRARMERIDRPDEVKEHPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALI1</accession>
    <name>I7ALI1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_020590</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264031.1">
        <property type="nucleotide sequence ID" value="XM_009265756.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALI1"/>
    <dbReference type="GeneID" id="20520819"/>
    <dbReference type="KEGG" id="ero:EROM_020590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020590"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_5_1"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12 16" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="128"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="303"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN82534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="507" mass="56827" checksum="13EF21F76D397E03" modified="2012-10-03" version="1">MKLELTVVEEGSRGGSIGLDRERASKRLLWASNDSGGQRSSESRKGKNGNALPKNLNKGSGSFEEKQKTTQKIPKRMEVPVVEDEDSKVKTSEKVKTLNSFKELCSQALVNALGKEYTVPTIIQKYCIPSMVEGRNLICRAPTGMGKTMCFLIPIIERYKQMKKPQACIISPTRELCEQIRTETSKLVYGSKIRVVSIYGKKQDLPSYAGVDIIVATPGRLIDLLHKGRVDLSEIKMFVLDEADKLLDMGFEVPIKEIHKYIPKNTQTCLFSATYSPKLTRMINYFLPEDKVSVEIPSETLRNIKQEIVEVRNKKKALLSILQNSDINLKGSWRMEVQPDKVLVFVERKSECGEVEKVLKKSGVLCVTLHGDKEQSDRNEALKGFRNGRFPVMVATSVAARGIDIKDIKLVINYDIPRDIKEYIHRIGRTGREGKSGKSISFYDGGVSADFKKALIEVLRESKNPVPSFLSDVAEDFDAKLQRLKISADKETSDDEEVGLWGDVSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALP0</accession>
    <name>I7ALP0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sec24-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_021140</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC23/SEC24 family. SEC24 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264086.1">
        <property type="nucleotide sequence ID" value="XM_009265811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALP0"/>
    <dbReference type="GeneID" id="20520876"/>
    <dbReference type="KEGG" id="ero:EROM_021140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021140"/>
    <dbReference type="HOGENOM" id="CLU_004589_2_1_1"/>
    <dbReference type="OrthoDB" id="49016at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050550">
        <property type="entry name" value="SEC23_SEC24_subfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13803">
        <property type="entry name" value="SEC24-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="4">
        <location>
            <begin position="72"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="5">
        <location>
            <begin position="160"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="7">
        <location>
            <begin position="385"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="6">
        <location>
            <begin position="493"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="705" mass="80378" checksum="4C4BE4B7F74B2CE8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALP6</accession>
    <name>I7ALP6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_021190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264091.1">
        <property type="nucleotide sequence ID" value="XM_009265816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALP6"/>
    <dbReference type="GeneID" id="20520881"/>
    <dbReference type="KEGG" id="ero:EROM_021190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021190"/>
    <dbReference type="HOGENOM" id="CLU_131614_0_0_1"/>
    <dbReference type="OrthoDB" id="2195889at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="175" mass="20072" checksum="DDE08123509F8735" modified="2012-10-03" version="1">MDERLKEVSRLMKEIKGKLEAIIQNSMHIKEFNYKAEALLAAFDAYTIYDPESNLGADRASLDIVSPRKNMQEEGTKEEVVLLPPSGIRKEESFEEFMERMESNTMCNKMFRANATKIAKFLYANKEGAALEEIIKTTGISRYRCVDILNSMLRTDPPLVSKRFDKGFIYSIVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALQ6</accession>
    <name>I7ALQ6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CAAX prenyl protease</fullName>
            <ecNumber evidence="15">3.4.24.84</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_021290</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Proteolytically removes the C-terminal three residues of farnesylated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>Hydrolyzes the peptide bond -P2-(S-farnesyl or geranylgeranyl)C-P1'-P2'-P3'-COOH where P1' and P2' are amino acids with aliphatic side chains and P3' is any C-terminal residue.</text>
            <dbReference type="EC" id="3.4.24.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14 15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Endoplasmic reticulum membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12 15">Belongs to the peptidase M48A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.84" evidence="15"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264101.1">
        <property type="nucleotide sequence ID" value="XM_009265826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALQ6"/>
    <dbReference type="GeneID" id="20520891"/>
    <dbReference type="KEGG" id="ero:EROM_021290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021290"/>
    <dbReference type="HOGENOM" id="CLU_025947_1_0_1"/>
    <dbReference type="OrthoDB" id="360839at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071586">
        <property type="term" value="P:CAAX-box protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07343">
        <property type="entry name" value="M48A_Zmpste24p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.10:FF:000002">
        <property type="entry name" value="CAAX prenyl protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027057">
        <property type="entry name" value="CAXX_Prtase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032456">
        <property type="entry name" value="Peptidase_M48_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10120">
        <property type="entry name" value="CAAX PRENYL PROTEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16491">
        <property type="entry name" value="Peptidase_M48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 15 18" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 14 15" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="169"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="CAAX prenyl protease 1 N-terminal" evidence="17">
        <location>
            <begin position="25"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="16">
        <location>
            <begin position="203"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR627057-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU366005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN82604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="411" mass="47751" checksum="6FB5462405FE3B00" modified="2012-10-03" version="1">MAGNFLLGISIMNYIFVVYLKTRELRQLSKPPSRTYLKLATPDQIKKTKEYNRDKLSMSIFELTLLLARDLYLINQGTLEKVYSSKYFMNNWYGDALFLMGYAHFQRLCDLPLEVISTFYIEAKHGFNKTTLSTFLMDFLKMSLVITVIFGPFSYVATKIIRIYHKTSFYLYLWVFMAIFQIVLVVVYPIVIQPLFNKFEEMEESDLKTKIEELAKKVGFCAKKILVMDASKRSGHSNAYFIGITKEKRVVIYDTLLKQTDEEEVLAILCHEFGHWKYNHVMKMASIVLLIQLFYLYVLNISLNSKSFERLVLAGDLPLLVRCIYFLMIIGALSVPIDTLRNFISRYFEGQADRFAVSLGYGKKLSSGLIKLFEKNSSNMEPDPLYSAVVHTHPTLIERMKLIENEMNKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM06</accession>
    <name>I7AM06_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin-related modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the URM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264186.1">
        <property type="nucleotide sequence ID" value="XM_009265911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM06"/>
    <dbReference type="GeneID" id="20520979"/>
    <dbReference type="KEGG" id="ero:EROM_030680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030680"/>
    <dbReference type="HOGENOM" id="CLU_148208_1_0_1"/>
    <dbReference type="OrthoDB" id="10248987at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015221">
        <property type="entry name" value="Urm1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09138">
        <property type="entry name" value="Urm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="MoaD/ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="96" mass="10938" checksum="01A8FCB1BD518B96" modified="2012-10-03" version="1">MIFKFNGASNVELNNKEIELTKEEMEGLPITTAEEALKYLFDRHMEVRDSYFDSHGELVYGTICIINKMDWEITGKEKSPVKYGDHAVLISTIHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM30</accession>
    <name>I7AM30_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_031070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264226.1">
        <property type="nucleotide sequence ID" value="XM_009265951.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM30"/>
    <dbReference type="GeneID" id="20521020"/>
    <dbReference type="KEGG" id="ero:EROM_031070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031070"/>
    <dbReference type="HOGENOM" id="CLU_042529_21_3_1"/>
    <dbReference type="OrthoDB" id="185659at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF128">
        <property type="entry name" value="THIOREDOXIN-DEPENDENT PEROXIDE REDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate; for peroxidase activity" evidence="4">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20284" checksum="12D7FB0E53D8BC45" modified="2012-10-03" version="1">MFSKVLSDSKYKAFVDGEIKEISLQDYKGKYVVVVFYPLDFTFVCPTEINRLSDLKEEFLKRNAVVLLVSCDSVYTHKAWASIPREKGGVLGVSWPMIWDAKRELCSQFSMFDEENGHPMRGTVLLSKDLSARHMSVNYPAIGRSVDEILRLIDALAFNEENGEICPVEWRKDVKNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM79</accession>
    <name>I7AM79_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264291.1">
        <property type="nucleotide sequence ID" value="XM_009266016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM79"/>
    <dbReference type="GeneID" id="20521089"/>
    <dbReference type="KEGG" id="ero:EROM_040250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040250"/>
    <dbReference type="HOGENOM" id="CLU_1586464_0_0_1"/>
    <dbReference type="OrthoDB" id="2190746at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="19802" checksum="DE497E6870B43B35" modified="2012-10-03" version="1">MSSLFMSMHERKFETNEYSMLIYTTGRGSILSCDSCVELITPVHINFPKEFILKENRIFCKIRIEGCYRRLKIVKIERMSGSCNAYTVLKRLIPSVFDVKFIRKANLQSYLHSNRDLIMRSSLRILGRETVDQINLWDRIKWQYGQDSEAPLDETKDVSSDILISDYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7AME9</accession>
    <name>I7AME9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable valine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Valyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_041120</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264376.1">
        <property type="nucleotide sequence ID" value="XM_009266101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AME9"/>
    <dbReference type="GeneID" id="20521176"/>
    <dbReference type="KEGG" id="ero:EROM_041120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041120"/>
    <dbReference type="HOGENOM" id="CLU_001493_0_0_1"/>
    <dbReference type="OrthoDB" id="629407at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000020">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000078">
        <property type="entry name" value="Valine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004349">
        <property type="entry name" value="PRK05729.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF109">
        <property type="entry name" value="VALINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="57"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="742"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN82879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="927" mass="106887" checksum="FF8FC939C05CCCC0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMM8</accession>
    <name>I7AMM8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264476.1">
        <property type="nucleotide sequence ID" value="XM_009266201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMM8"/>
    <dbReference type="GeneID" id="20521278"/>
    <dbReference type="KEGG" id="ero:EROM_050460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050460"/>
    <dbReference type="HOGENOM" id="CLU_124045_0_0_1"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="186" mass="21447" checksum="D9161E55CCC20950" modified="2012-10-03" version="1">MGTVTKKNKEKRQAEMEKIRNYAEKYPLVAVIENPGIPNVILKKIREDFKGTSKILFTRKKMASAVYNMPSMPADSFFLMFGDEGAIEKAKSYEYEDFLEENDICPSRVVIEKQVVRNKEMIEILPVTMKDEKMQLNEDYVVCEAGETVDERKAKILKFFGKRLKKKNLVVFDIASTSTLLKSKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN67</accession>
    <name>I7AN67_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Preprotein translocase subunit Sec63</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_060810</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264671.1">
        <property type="nucleotide sequence ID" value="XM_009266396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN67"/>
    <dbReference type="GeneID" id="20521477"/>
    <dbReference type="KEGG" id="ero:EROM_060810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060810"/>
    <dbReference type="HOGENOM" id="CLU_494335_0_0_1"/>
    <dbReference type="OrthoDB" id="1734229at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006620">
        <property type="term" value="P:post-translational protein targeting to endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075">
        <property type="entry name" value="SEC63 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24075:SF0">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC63 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="9">
        <location>
            <begin position="90"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="552" mass="63364" checksum="2C21CA31C8C293D7" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANE0</accession>
    <name>I7ANE0_ENCRO</name>
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        <recommendedName>
            <fullName evidence="2">separase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_070280</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.</text>
            <dbReference type="EC" id="3.4.22.49"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.22.49" evidence="2"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264776.1">
        <property type="nucleotide sequence ID" value="XM_009266501.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANE0"/>
    <dbReference type="GeneID" id="20521587"/>
    <dbReference type="KEGG" id="ero:EROM_070280"/>
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    <dbReference type="HOGENOM" id="CLU_355297_0_0_1"/>
    <dbReference type="OrthoDB" id="10255632at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051307">
        <property type="term" value="P:meiotic chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51700">
        <property type="entry name" value="SEPARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase C50" evidence="5">
        <location>
            <begin position="612"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="774" mass="90069" checksum="1E9716A3E02993C6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANE9</accession>
    <name>I7ANE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_070380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264786.1">
        <property type="nucleotide sequence ID" value="XM_009266511.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANE9"/>
    <dbReference type="GeneID" id="20521597"/>
    <dbReference type="KEGG" id="ero:EROM_070380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070380"/>
    <dbReference type="HOGENOM" id="CLU_561424_0_0_1"/>
    <dbReference type="OrthoDB" id="21095at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015187">
        <property type="entry name" value="BRCA2_OB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002093">
        <property type="entry name" value="BRCA2_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09103">
        <property type="entry name" value="BRCA-2_OB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00634">
        <property type="entry name" value="BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BRCA2 OB1" evidence="5">
        <location>
            <begin position="253"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="487" mass="56506" checksum="2B02F3B426120DD0" modified="2012-10-03" version="1">MDADSEVEFESFFQSSSNNRESEDSSSGFGSIQMSCDEMAFFSSDNALHEDNRAKEEESCESVDQGNQTFYDFNSDSSRIRENPEEEGCGDVNIAFPQGSNVLDSYENQITNSPRKHLSFDLDQLHVRHMDSGNAGSDTIFSGFTTGSRKKIQVRKESMDSAYRTFDGAAKKVDMQTLSRFYDISKDESEYMGPERVYEEIKRRLVKEDANRVFAQFTWSWIYFFFGRKKMEFDDLVDRIEEQVKVRLENEYSILRRIVEGDEVPWRYMILLVIGIDKERIEVFDGYYSLYALYDEPLRRKIEKKEIRVGLKLKIFGAELGGGEFMSIFDPESAFLRLHHNGVQVVHSRRRLGYRKRVGFKIRISDIRNDGGPVSCIEGRIAKIIETKYLVKVENYSCTTENLEPELDKIEELAKKAQRIFSSHDIRISMYTRLLVRDSSGECMVTWWNPSSDAKVGQMVRMVYLNPSRAKELHLNTSRRGYVKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANM4</accession>
    <name>I7ANM4_ENCRO</name>
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        <submittedName>
            <fullName evidence="11">23S rRNA methyl-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_071280</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264876.1">
        <property type="nucleotide sequence ID" value="XM_009266601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANM4"/>
    <dbReference type="GeneID" id="20521688"/>
    <dbReference type="KEGG" id="ero:EROM_071280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071280"/>
    <dbReference type="HOGENOM" id="CLU_009422_8_2_1"/>
    <dbReference type="OrthoDB" id="1287559at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000466">
        <property type="term" value="P:maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000004">
        <property type="entry name" value="AdoMet-dependent rRNA methyltransferase SPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050082">
        <property type="entry name" value="RNA_methyltr_RlmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024576">
        <property type="entry name" value="rRNA_MeTfrase_Spb1_DUF3381"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920:SF13">
        <property type="entry name" value="PRE-RRNA 2'-O-RIBOSE RNA METHYLTRANSFERASE FTSJ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10920">
        <property type="entry name" value="RIBOSOMAL RNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11861">
        <property type="entry name" value="DUF3381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA methyltransferase FtsJ" evidence="9">
        <location>
            <begin position="24"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3381" evidence="10">
        <location>
            <begin position="221"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="315"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="461"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF11861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="551" mass="64015" checksum="1A0B124582D0F9FE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANT8</accession>
    <name>I7ANT8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_080320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264951.1">
        <property type="nucleotide sequence ID" value="XM_009266676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANT8"/>
    <dbReference type="GeneID" id="20521768"/>
    <dbReference type="KEGG" id="ero:EROM_080320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080320"/>
    <dbReference type="HOGENOM" id="CLU_005316_4_3_1"/>
    <dbReference type="OrthoDB" id="6093671at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808">
        <property type="entry name" value="NCL1 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22808:SF1">
        <property type="entry name" value="RNA CYTOSINE-C(5)-METHYLTRANSFERASE NSUN2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="24"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="121"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="515" mass="58333" checksum="B24A838E4CDE6D63" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP17</accession>
    <name>I7AP17_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromatin assembly factor 1 subunit P60</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265041.1">
        <property type="nucleotide sequence ID" value="XM_009266766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP17"/>
    <dbReference type="GeneID" id="20521863"/>
    <dbReference type="KEGG" id="ero:EROM_081280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081280"/>
    <dbReference type="HOGENOM" id="CLU_760826_0_0_1"/>
    <dbReference type="OrthoDB" id="71227at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033186">
        <property type="term" value="C:CAF-1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045145">
        <property type="entry name" value="PTHR15271"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15271:SF4">
        <property type="entry name" value="CHROMATIN ASSEMBLY FACTOR 1 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="361" mass="40760" checksum="F90FBAE6C10B1BF6" modified="2012-10-03" version="1">MEGLKKWYPIEVTTHNLHFHEEASIFTMHGNEKFIATGGGDKDIRLWRVEKDTCKGIDFCYTTAIDSSIKIKYHAVLSGHHRSVNCVRFNGDILASCSDGGEVILWKGRKAYVVRRIDGDDAYELVWGCRHLFVGFSSGNILVYQVTSDNNFLDTADAINEGEFNEASDSECSPGKEKDCKQGISTKLVQKIKCHEDIIQGLAFNHHFNVLTSLSKDCTGKTFLFTDKLIEIEKMEYHDGNKLFSTGRGFFRRLSYSPDGKLLYLVCCRSNSVVVLHYPFRMEHVYATIGPFDSEPLKVVCGEDRVFITTKKSLYMVAHNKPALCVDNISFMTITDGHVFDGVCFLSSLDGFLSSIRMKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP58</accession>
    <name>I7AP58_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265091.1">
        <property type="nucleotide sequence ID" value="XM_009266816.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP58"/>
    <dbReference type="GeneID" id="20521913"/>
    <dbReference type="KEGG" id="ero:EROM_081780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081780"/>
    <dbReference type="HOGENOM" id="CLU_1245343_0_0_1"/>
    <dbReference type="OrthoDB" id="10655002at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="229" mass="24643" checksum="899482AF5E22AB2C" modified="2012-10-03" version="1">MKKLLFASGILIVKAFKRLHGTSHHRSGLMNLKKHHHLNLKRSNLSSVSNVKDKGDSNGGNGNNNKKEKKKDKEGGKESDKEKGDDKGDGKKEDKEEDKEEGKGSSKSSGENKNNESGKSGSSLTNHSSNLSSPDAQAGSTDGKKNSTKVSTGPTKEDMNNSQVEKKKAQEKRNSTRSSPQGTKKEAGDSVDTFNTNKKKGNLKKSGINESSNLLDSEIDSDASVVENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I7APG5</accession>
    <name>I7APG5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Small RNA-binding pol III transcript stabilizing-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_091080</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265221.1">
        <property type="nucleotide sequence ID" value="XM_009266946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APG5"/>
    <dbReference type="GeneID" id="20564332"/>
    <dbReference type="KEGG" id="ero:EROM_091080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091080"/>
    <dbReference type="HOGENOM" id="CLU_067903_0_0_1"/>
    <dbReference type="OrthoDB" id="439993at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07323">
        <property type="entry name" value="LAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12291">
        <property type="entry name" value="RRM1_La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045180">
        <property type="entry name" value="La_dom_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006630">
        <property type="entry name" value="La_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002344">
        <property type="entry name" value="Lupus_La"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792:SF140">
        <property type="entry name" value="ACHILLES, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05383">
        <property type="entry name" value="La"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00302">
        <property type="entry name" value="LUPUSLA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00715">
        <property type="entry name" value="LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="HTH La-type RNA-binding" evidence="7">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="97"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="34599" checksum="75EB582C52ADAD17" modified="2012-10-03" version="1">MASEKLEKIRSQVEFYFSDANFRVDKFLREQSLVNDGYIPIKTIVSFNRLRSLEATVEDVKEALKDSKVVEVKDEMVKKIETEEYLSYVMEKDISKRVIYIDGFPKDMMLEDVKDTLSPFITPVLIRMRRDKGKNFSGSIFAEMKSEEEAQKALSEKIPAKKQSDDEEKAKKQKSEEKYLVIMTKEEYLKEKEKMSAEKKEKEAREALEKEFVPKLFRYESDSDLDIKAVKSLVKNSAFVDTKEKVVRMKHAEDFKEKKFENEGRSLTLIKMEEGEALEYCKNIKVTPKKPKSAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7API1</accession>
    <name>I7API1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rad52-like DNA repair and recombination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RAD52 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265246.1">
        <property type="nucleotide sequence ID" value="XM_009266971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7API1"/>
    <dbReference type="GeneID" id="20564357"/>
    <dbReference type="KEGG" id="ero:EROM_091330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091330"/>
    <dbReference type="HOGENOM" id="CLU_100405_0_0_1"/>
    <dbReference type="OrthoDB" id="206565at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000730">
        <property type="term" value="P:DNA recombinase assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045002">
        <property type="term" value="P:double-strand break repair via single-strand annealing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006312">
        <property type="term" value="P:mitotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.80:FF:000001">
        <property type="entry name" value="DNA repair protein RAD52 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.80">
        <property type="entry name" value="DNA repair protein Rad52/59/22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004585">
        <property type="entry name" value="DNA_recomb/repair_Rad52"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041247">
        <property type="entry name" value="Rad52_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007232">
        <property type="entry name" value="Rad52_Rad59_Rad22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042525">
        <property type="entry name" value="Rad52_Rad59_Rad22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00607">
        <property type="entry name" value="rad52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RAD52, RAD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12132:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD52 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04098">
        <property type="entry name" value="Rad52_Rad22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="204" mass="23288" checksum="73B648147919D4D1" modified="2012-10-03" version="1">MREDELRGWRSGTFGRKRVRKISKLLNQKLGPEFISYRPGFNMSKIAYVEGWKVISLANRIFGFNGWSSEIRTMSVDYEEADEKKISLGVSCLVRVMLKDGTYREDIGFGSAENQRSRAAAYEKAKKEAATDALKRALRQFGNSLGNCCYDKNYIKEVQRIHKINEKALDSNDLFRNNGGVSEESDREGDASFDIDYHDLSDTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7APN6</accession>
    <name>I7APN6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">U1 snRNP-specific protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_100160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265331.1">
        <property type="nucleotide sequence ID" value="XM_009267056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APN6"/>
    <dbReference type="GeneID" id="20564446"/>
    <dbReference type="KEGG" id="ero:EROM_100160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100160"/>
    <dbReference type="HOGENOM" id="CLU_164885_0_0_1"/>
    <dbReference type="OrthoDB" id="2417221at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="124" mass="14639" checksum="7A93B3E526DA6364" modified="2012-10-03" version="1">MAKYFCEFCNKTLLNDKLRSRRIHFQGAKHKLMRKAYYMEIFEKPEVAVEMASILRDIKAEDKRIEQEDLEKKQGSCFSLPPGDFYLGLDLPEEPFGFKLPSGFDFQDRKNFPENITEAIKKYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7APQ6</accession>
    <name>I7APQ6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265356.1">
        <property type="nucleotide sequence ID" value="XM_009267081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APQ6"/>
    <dbReference type="GeneID" id="20564473"/>
    <dbReference type="KEGG" id="ero:EROM_100430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100430"/>
    <dbReference type="HOGENOM" id="CLU_082477_0_0_1"/>
    <dbReference type="OrthoDB" id="191139at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047560">
        <property type="term" value="F:3-dehydrosphinganine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006666">
        <property type="term" value="P:3-keto-sphinganine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43550:SF3">
        <property type="entry name" value="3-KETODIHYDROSPHINGOSINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="236" mass="27313" checksum="FF4D8834E476DDE0" modified="2012-10-03" version="1">MSKVNLAGKTVCLTGGSRGLGLEIAKELAKKGSYVHVIGKSPQSFGHENIKYYQIDLLKEKPDITTPFDIVISNLGTSVGNKRFDDMEYNEVMDMLKLNVELHLWLLKNVRYKKFVFVNSILSQQGLPEYSMYCASKSFIHTLNQSLRREGKDTMIIYPYKINTPLFREVRDVWTLDANYVAVRLLKDIENGVREDFVPWIFKPIILISRMIPMFIQDRLSSLVKKTFYNSEEKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7APT9</accession>
    <name>I7APT9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoribonuclease YSH1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Endoribonuclease ysh1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">mRNA 3'-end-processing protein YSH1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_100780</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. CPSF2/YSH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265391.1">
        <property type="nucleotide sequence ID" value="XM_009267116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APT9"/>
    <dbReference type="GeneID" id="20564508"/>
    <dbReference type="KEGG" id="ero:EROM_100780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100780"/>
    <dbReference type="HOGENOM" id="CLU_009673_2_3_1"/>
    <dbReference type="OrthoDB" id="10249535at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006398">
        <property type="term" value="P:mRNA 3'-end processing by stem-loop binding and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16292">
        <property type="entry name" value="CPSF3-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000001">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021718">
        <property type="entry name" value="CPSF73-100_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF11">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11718">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01098">
        <property type="entry name" value="CPSF73-100_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="11">
        <location>
            <begin position="19"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="12">
        <location>
            <begin position="241"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA 3'-end-processing endonuclease polyadenylation factor C-term" evidence="13">
        <location>
            <begin position="465"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="643" mass="72564" checksum="49937F96543724B1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7APY3</accession>
    <name>I7APY3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Beta-lactamase fold-containingexonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_101240</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. INTS11 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265436.1">
        <property type="nucleotide sequence ID" value="XM_009267161.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APY3"/>
    <dbReference type="GeneID" id="20564554"/>
    <dbReference type="KEGG" id="ero:EROM_101240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101240"/>
    <dbReference type="HOGENOM" id="CLU_009673_3_2_1"/>
    <dbReference type="OrthoDB" id="10249535at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016180">
        <property type="term" value="P:snRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16291">
        <property type="entry name" value="INTS11-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10890:FF:000002">
        <property type="entry name" value="Integrator complex subunit 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000028">
        <property type="entry name" value="Integrator complex subunit 11 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041897">
        <property type="entry name" value="INTS11-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF37">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="13"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="11">
        <location>
            <begin position="240"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="496" mass="56439" checksum="7998405ABF25363A" modified="2012-10-03" version="1">MNVVPLGAGQDVGRSCVLVTIGGRTIMFDCGMHMGFNDERRFPDFSYISKTKSFDKAIDCVVISHFHLDHCGALPYFTEVCGYNGPVYMTLPTKEVCPVLLDDFRKIVEGKGDSIFTYQDILNCMKKVTTINMNETYKHDEDFYITPYYAGHVLGAAMFHVVVGDQSVVYTGDYSTTPDKHLGPASIKCVRPDLLITESTYGSITRDCRRVKEREFLKAVSDCIARGGRVLIPIFALGRAQELCLLLDGYWERTGLKIPVYFSSGLTEKANEIYKKFISYTNETVKRKIFERNVFEYKHIKPFQKYYMENKGPMVLFASPGMLHSGMSLRMFKEWCEDEKNLVIIPGYCVRGTIGEKVLNGAKRLEVLGEEKDIRLEVRNLAFSAHADAQGILNVIEQCSPRNVMLVHGERSRMKQLKRNIEERFGIPTFLPPNGTLINIPSKNTINLRIRKESISKYFKPSLSKSKVSLRMKLSSEKKGNYIDVMSCEEYIEDNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ69</accession>
    <name>I7AQ69_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_110420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265521.1">
        <property type="nucleotide sequence ID" value="XM_009267246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ69"/>
    <dbReference type="GeneID" id="20564641"/>
    <dbReference type="KEGG" id="ero:EROM_110420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110420"/>
    <dbReference type="HOGENOM" id="CLU_1948793_0_0_1"/>
    <dbReference type="OrthoDB" id="2191834at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707922" evidence="2">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="129" mass="14880" checksum="F3A3DBFB62BBCC95" modified="2012-10-03" version="1">MRFIGVLASLLLSTILCIVELYKIEAVVDVESRRASSYNACDRILRLADVEFKLLVLLMVAQTLSLSYTSLLTVLVFVYELQKKGSETTISALNFFKQKRWIKIEIFCKTSFYVLLLYKYFSTLIVNKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ78</accession>
    <name>I7AQ78_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_011230</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263964.1">
        <property type="nucleotide sequence ID" value="XM_009265689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ78"/>
    <dbReference type="GeneID" id="20520750"/>
    <dbReference type="KEGG" id="ero:EROM_011230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011230"/>
    <dbReference type="HOGENOM" id="CLU_002572_0_1_1"/>
    <dbReference type="OrthoDB" id="2186317at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF16">
        <property type="entry name" value="CHITIN SYNTHASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="695"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="722"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="748"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="816" mass="93399" checksum="1ADBBDA9F7822487" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQA1</accession>
    <name>I7AQA1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">GATA zinc finger transcription factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_020170</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263989.1">
        <property type="nucleotide sequence ID" value="XM_009265714.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQA1"/>
    <dbReference type="GeneID" id="20520775"/>
    <dbReference type="KEGG" id="ero:EROM_020170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020170"/>
    <dbReference type="HOGENOM" id="CLU_813880_0_0_1"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="Transcription_factor_GATA"/>
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    </dbReference>
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        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071:SF281">
        <property type="entry name" value="BOX A-BINDING FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10071">
        <property type="entry name" value="TRANSCRIPTION FACTOR GATA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00320">
        <property type="entry name" value="GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00401">
        <property type="entry name" value="ZnF_GATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00344">
        <property type="entry name" value="GATA_ZN_FINGER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50114">
        <property type="entry name" value="GATA_ZN_FINGER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GATA-type" evidence="8">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="341" mass="38588" checksum="4A86ABAA954715B3" modified="2012-10-03" version="1">MTSVNKDDGGKRHIEDTQDKDLSDVEERRGFHKDDMNVEMEDIKGCEKKVFDGKEMEYGNRCHLKSFLQGVDGIKNYNRTVKICPGSDRLSSCKEDKDRGLISKYDAIEIPKITRELKRKAKQRICSNCSTTSTPSWRRGDQGKTLLCNACGLYQKLHGRTRPYMITSGGRTKALKGSHERIVCVSCNISFFTSDSKNAPSHLCESCLDYARSRRDQPDNSGEDERRNGFNGKVLGNRYGGVYDREVSEKYNPSCNYIGRDVMINRFRDYSSPSVAYPQPYGYYYLNEKYHDTPGFGGYGPSFPSRDLDTQFKDIYSGEMVSEGESGMDSCDANVNTPASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQB1</accession>
    <name>I7AQB1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263999.1">
        <property type="nucleotide sequence ID" value="XM_009265724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQB1"/>
    <dbReference type="GeneID" id="20520785"/>
    <dbReference type="KEGG" id="ero:EROM_020270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020270"/>
    <dbReference type="HOGENOM" id="CLU_1749621_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="149" mass="17685" checksum="F72E4F3D3577CE98" modified="2012-10-03" version="1">MVKEEGCKYSMCLNKMFFGYRKHLWFYGRLEFSGKFEKFTLEKERSPKTCVQQDRRSKAKRPGRSLKVRKKPLKPPILLNILMRALMGPAILRLTQMIHLMETRTPALMMRCLMMMETTIPMTWKEIVGLMKNPIRNKVLLKLDTLLLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQF2</accession>
    <name>I7AQF2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_020670</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264039.1">
        <property type="nucleotide sequence ID" value="XM_009265764.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQF2"/>
    <dbReference type="GeneID" id="20520827"/>
    <dbReference type="KEGG" id="ero:EROM_020670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020670"/>
    <dbReference type="HOGENOM" id="CLU_055193_1_1_1"/>
    <dbReference type="OrthoDB" id="29563at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001921">
        <property type="entry name" value="Ribosomal_eL8_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00882">
        <property type="entry name" value="RIBOSOMALL7A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="5">
        <location>
            <begin position="90"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="206" mass="23136" checksum="E82B974F5CF9162D" modified="2012-10-03" version="1">MVLGKVKKIPLIAPRLTKEQKMEKELLIKKKINKLANAIRIPPAINQFKTFLSEDDTKKFINLFMNYRPENKEEKKARLKSEDPKKGPKPILVKFGLKHVTNLIETKKAKLVLISASVDPIEVVIFLPTLCRKMGVSYAIVEDSTILGRLVNLKSTSCVCLCDVRPEDEAAFKEILGIANATFLDSYETHLSTWGGGPKKQTTGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQK1</accession>
    <name>I7AQK1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_021220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264094.1">
        <property type="nucleotide sequence ID" value="XM_009265819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQK1"/>
    <dbReference type="GeneID" id="20520884"/>
    <dbReference type="KEGG" id="ero:EROM_021220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021220"/>
    <dbReference type="HOGENOM" id="CLU_950032_0_0_1"/>
    <dbReference type="OrthoDB" id="2191075at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="293" mass="34363" checksum="19B83FE2CE555135" modified="2012-10-03" version="1">MILDYGIFFSRKVSVCLYEGHMLVGNRTAFTAIKMEFIDKHHAFLHPNAKRKRSYVEKIASDPRLLLRSKRNPKENLKLYVSLVTKGRYRPVIRGNKKQRLIEDAIRGKEVAWRSIATKDIYGYDKLMNVLNSDPNPSSEMTLKRKIMSMFKISTDEKDGMDEGKIICDVLKNEKEMPKEIGFSLRVHYDFCIKNKTVSLLDEIDREYDKMLGPVLWNKIDNEVKRCEIDHPFYSEVGLFNLFVKCKDNIPDFDRKVLEKDVFHTINERGIRDFIAERVERFYAAGVYLSNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQM4</accession>
    <name>I7AQM4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ethanolamine kinase</fullName>
            <ecNumber evidence="3">2.7.1.82</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the choline/ethanolamine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.82" evidence="3"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264119.1">
        <property type="nucleotide sequence ID" value="XM_009265844.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQM4"/>
    <dbReference type="GeneID" id="20520910"/>
    <dbReference type="KEGG" id="ero:EROM_030030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030030"/>
    <dbReference type="HOGENOM" id="CLU_888755_0_0_1"/>
    <dbReference type="OrthoDB" id="10267235at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004305">
        <property type="term" value="F:ethanolamine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603">
        <property type="entry name" value="CHOLINE/ETHANOALAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22603:SF66">
        <property type="entry name" value="ETHANOLAMINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01633">
        <property type="entry name" value="Choline_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="344" mass="39399" checksum="E265272D94137D1D" modified="2012-10-03" version="1">MVRRRDGKLCGDEELLSKEPGVDTTAIGGKQEPGVPQEVIKDIRMCIGGEPSGMSRIRTAYSNVVYMFEVNGSKYIYKEFREFDEQDEMINRIVGGERVVASNHRFRIEKRLEGRHGSIREDLRRIAPELRKFHDTHVEGIRSYEELVESMMVKCIREMVAGSGRGDMSLSSIEEKMKRMRASDAGWCSGDDCVFWILMKARDRVFEVPKGGFKAVMCHNDLQPGNILVSKDEVVLIDFEFAAMGSPVIEIANLFCEAGYDYSRYVFLEERFPSAEEQMEFVREYMGGDESWLEIVRLVPGAMAYSHFLWYLWAWCNKRCGSSEVLTTVGMETHDCHGYMMTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQR0</accession>
    <name>I7AQR0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L31</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264129.1">
        <property type="nucleotide sequence ID" value="XM_009265854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQR0"/>
    <dbReference type="GeneID" id="20520920"/>
    <dbReference type="KEGG" id="ero:EROM_030130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030130"/>
    <dbReference type="HOGENOM" id="CLU_112570_1_3_1"/>
    <dbReference type="OrthoDB" id="9739313at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="110" mass="12762" checksum="2F465C4921FC8F9F" modified="2012-10-03" version="1">MWSRIEENQTVEMTVNLRRICSRLPWTKKADKAVKMLKREIQKHFKEKIGVAITSELNNFIHSRGTKKIPSKVRVRVTKESSLKNAEENVLKTDLVIVGSFKNLKEVIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQT6</accession>
    <name>I7AQT6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar import and degradation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GID4/VID24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264164.1">
        <property type="nucleotide sequence ID" value="XM_009265889.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQT6"/>
    <dbReference type="GeneID" id="20520957"/>
    <dbReference type="KEGG" id="ero:EROM_030460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030460"/>
    <dbReference type="HOGENOM" id="CLU_028759_2_1_1"/>
    <dbReference type="OrthoDB" id="62at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045721">
        <property type="term" value="P:negative regulation of gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007039">
        <property type="term" value="P:protein catabolic process in the vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018618">
        <property type="entry name" value="GID4/10-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534:SF3">
        <property type="entry name" value="GID COMPLEX SUBUNIT 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14534">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09783">
        <property type="entry name" value="Vac_ImportDeg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="185" mass="21806" checksum="0C67FE2BDEF146B8" modified="2012-10-03" version="1">MHSKEALALENGAKYRGEQLTNGGSFKIEMNVDIIDTRQEFICGTFEIHNLTDRYELLTTYFEGEIVGNIYPFTTEEYSMANPDIIHWKLFPEWKGEYVYRKGSYDIRKSNFMYIKIKELFLLPDPRLKTIPGASIDGHYYCCYYKNLDCFAGHYYYKNTSNLISQQVLLDRVFPRKSRSKSFSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQV0</accession>
    <name>I7AQV0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264179.1">
        <property type="nucleotide sequence ID" value="XM_009265904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQV0"/>
    <dbReference type="GeneID" id="20520972"/>
    <dbReference type="KEGG" id="ero:EROM_030610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030610"/>
    <dbReference type="HOGENOM" id="CLU_118652_1_1_1"/>
    <dbReference type="OrthoDB" id="277449at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="114" mass="12941" checksum="3981653F67C30AA6" modified="2012-10-03" version="1">MKNVLIHKGSTYKTRSNRRVRVRTPSGKLVNRRTKKYSKKHRCHECNAVLGSIARMRPAEFSRQKVSARRVNRAYGATHCGRCVREKIIGAFLADEERIVKEKTGMNIGMAIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR15</accession>
    <name>I7AR15_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264279.1">
        <property type="nucleotide sequence ID" value="XM_009266004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR15"/>
    <dbReference type="GeneID" id="20521075"/>
    <dbReference type="KEGG" id="ero:EROM_040110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040110"/>
    <dbReference type="HOGENOM" id="CLU_1677871_0_0_1"/>
    <dbReference type="OrthoDB" id="2573941at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="155" mass="18146" checksum="6927C64149DA6D4D" modified="2012-10-03" version="1">MGGSKAVAISNIHPRIRLRDIMDIFGVFDKIVGFRPYKDGFVLKFESFPERSLAMDNFPLAHRRMKVNMIEWNEGYDTFEVGNIVVFESYFDVDEIRDECCMFGCVTEVIRKKDSIYVICSSSVDGECIFMNMYGRYYNKKRIRCRIGDEKILLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARM3</accession>
    <name>I7ARM3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylinositol transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264524.1">
        <property type="nucleotide sequence ID" value="XM_009266249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARM3"/>
    <dbReference type="GeneID" id="20521328"/>
    <dbReference type="KEGG" id="ero:EROM_050960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050960"/>
    <dbReference type="HOGENOM" id="CLU_046509_0_0_1"/>
    <dbReference type="OrthoDB" id="18453at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005548">
        <property type="term" value="F:phospholipid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001666">
        <property type="entry name" value="PI_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055261">
        <property type="entry name" value="PI_transfer_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10658:SF11">
        <property type="entry name" value="VIBRATOR, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02121">
        <property type="entry name" value="IP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00391">
        <property type="entry name" value="PITRANSFER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphatidylinositol transfer protein N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="252" mass="29491" checksum="0262C8BF681BCD82" modified="2012-10-03" version="1">MGKVEKIYVTVLPMTTDEYAVGHFHTVSSMTESENAGDLKVEMIKAETVVHETLGKANKTHKIMYLKSRFPQVLHSLIPEDACIVEEISYNAYTKCHTFYKNRYFSNETFNMAFNTINRDGAEILENPFEHDPEHIRNIEKINLDLHSSPINPMFDPSVYYHEESGRGRLDEHWMEGYKNKGIPMMVSYKHLTVEINNYMMGWISAEIEKVMRGVITSVQQRIFCTMDKWYGKTIDEIRKENENQKDKNFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARU9</accession>
    <name>I7ARU9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation initiation factor IF2 subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264604.1">
        <property type="nucleotide sequence ID" value="XM_009266329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARU9"/>
    <dbReference type="GeneID" id="20521409"/>
    <dbReference type="KEGG" id="ero:EROM_060120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060120"/>
    <dbReference type="HOGENOM" id="CLU_026663_3_2_1"/>
    <dbReference type="OrthoDB" id="10255414at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
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        <source>
            <dbReference type="EMBL" id="AFN83117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="527" mass="61800" checksum="9548C084271D4361" modified="2012-10-03" version="1"> </sequence>
</entry>
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    <name>I7AST6_ENCRO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="ordered locus">EROM_080890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Fungi incertae sedis</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2192253at2759"/>
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    </dbReference>
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        <location>
            <begin position="61"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="144" mass="16384" checksum="C9CF8555A0852939" modified="2012-10-03" version="1">MKFNNLMDILKEKYNVKRMSDGTCVISNGNNHVKIHEEELNSSFDINKLLEKLDLPTEGFEHVGRDDLFPNTNGVNMGKIGKRHPKGSFMDPSAFSSPSRQSPESEDSNPLVIIDPITPSRNKKDSFEPDPDNYNPDRNSRFPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASU9</accession>
    <name>I7ASU9_ENCRO</name>
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        </submittedName>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
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            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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    <dbReference type="HOGENOM" id="CLU_1199813_0_0_1"/>
    <dbReference type="OrthoDB" id="2193288at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="229" mass="25821" checksum="DD663FEAF1AEAB43" modified="2012-10-03" version="1">MYEELLNFLLARKKRLLSEEEADKLMQLIAQSMEIEDGQREKEDNTALIKKILDNVDVDKIIEEDSESMEDAIDDLFFNDYSNEDCSDDIIDLCESDYAGESLSERKFQRGVFRTKGGSDVASEAPKRKIGIKTRKSDTEGLNEEDVMIDKHGGAPKDEVNGGMKALETRCFKLPGSDTNGSHLGSNEERRKGSDLLESSEFQFDLGDLKRVDNTGPDVKFFDEDGKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT39</accession>
    <name>I7AT39_ENCRO</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">EROM_082030</name>
    </gene>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Encephalitozoon</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265114.1">
        <property type="nucleotide sequence ID" value="XM_009266839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT39"/>
    <dbReference type="GeneID" id="20521938"/>
    <dbReference type="KEGG" id="ero:EROM_082030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_082030"/>
    <dbReference type="HOGENOM" id="CLU_1042166_0_0_1"/>
    <dbReference type="OrthoDB" id="2195697at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
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        <property type="entry name" value="DUF1686"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07937">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="29901" checksum="948F942868B9FE83" modified="2012-10-03" version="1">MNENSSSSHQSGGLWQSAFRKLEVFKSQFNNLYSSLLGSKDNDPSSVPESAEADFIDINPFNRNFFFILYCLTTLGLLFFSCIVKGSVQSNKKMEMGIDVLLFLVSVAGPIMYGMWILMNEYNDNGIENVIKNNWNIVGSMIPIIIGILNGKVTRRYRIMMAGLGIIMIYVQKIVQEKIHKGQLLTLIIGNAVMISVYLIKLVNEGFEDEYEIVICIVPIVIAIGLIVIDMLEEKKEGSEVNSEMKNIVCVMSWIVGTIGWYLGPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATT4</accession>
    <name>I7ATT4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265394.1">
        <property type="nucleotide sequence ID" value="XM_009267119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATT4"/>
    <dbReference type="GeneID" id="20564511"/>
    <dbReference type="KEGG" id="ero:EROM_100810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100810"/>
    <dbReference type="HOGENOM" id="CLU_519737_0_0_1"/>
    <dbReference type="OrthoDB" id="2196371at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003228">
        <property type="entry name" value="TFIID_TAF12_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03847">
        <property type="entry name" value="TFIID_20kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 12" evidence="2">
        <location>
            <begin position="451"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="524" mass="59585" checksum="F7018C497EB08D2D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU64</accession>
    <name>I7AU64_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molecular chaperone DnaK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_110400</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265519.1">
        <property type="nucleotide sequence ID" value="XM_009267244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU64"/>
    <dbReference type="GeneID" id="20564639"/>
    <dbReference type="KEGG" id="ero:EROM_110400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110400"/>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_1"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10234">
        <property type="entry name" value="ASKHA_NBD_HSP70_DnaK-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000012">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000004">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000003">
        <property type="entry name" value="Molecular chaperone DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="593" mass="64674" checksum="1A97772B1030E3F8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUA3</accession>
    <name>I7AUA3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation initiation factor 2 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_110800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265559.1">
        <property type="nucleotide sequence ID" value="XM_009267284.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUA3"/>
    <dbReference type="GeneID" id="20564679"/>
    <dbReference type="KEGG" id="ero:EROM_110800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110800"/>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_1"/>
    <dbReference type="OrthoDB" id="1685042at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="4">
        <location>
            <begin position="17"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="283" mass="32151" checksum="F5F9AD38C1B07BB5" modified="2012-10-03" version="1">MMKYECRLHFRKYPKEGEIVMGRVVSIGGDGLTLNLLEYGDLEGLVLLGELSKRRVKNIQQVTKVGNIEICNVLKVDEEKGYIDLSMSKVTENEKVECKETSAKNKLAYHIMFKAAKRLKMEVSDLYKKMGYDKEEEFGSLYYFFARVKDNGDIMDDDDIGLTIKNLIKEQFQASTYKVRADIDVICSSKGGINSIKKVFAEALGLDPRLEFYLIKTPTYSVTRVSSNKDKAYEVVREACNVLISNIQKEGGTATVISQPKLYGEKSRYNLLNFEENEVSSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUF9</accession>
    <name>I7AUF9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Class 2 transcription repressor NC2 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265614.1">
        <property type="nucleotide sequence ID" value="XM_009267339.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUF9"/>
    <dbReference type="GeneID" id="20564735"/>
    <dbReference type="KEGG" id="ero:EROM_111360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111360"/>
    <dbReference type="HOGENOM" id="CLU_045277_9_0_1"/>
    <dbReference type="OrthoDB" id="653904at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001046">
        <property type="term" value="F:core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22906">
        <property type="entry name" value="HFD_DRAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF5">
        <property type="entry name" value="DR1-ASSOCIATED COREPRESSOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="3">
        <location>
            <begin position="13"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="95" mass="11004" checksum="EC8B541C20065194" modified="2012-10-03" version="1">MPRQESQKKKLTRFPISRLKRIMQLNEDIGKIGASVPVVASKAIEMFLTEIVELTLKEARKKSSSRMSPEFITRAIESSPKFEFLKNMEQFKSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUG3</accession>
    <name>I7AUG3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transfer RNA methyltransferase 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TRM8</name>
        <name evidence="10" type="ordered locus">EROM_111410</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with TRM82.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="9"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265619.1">
        <property type="nucleotide sequence ID" value="XM_009267344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUG3"/>
    <dbReference type="GeneID" id="20564740"/>
    <dbReference type="KEGG" id="ero:EROM_111410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111410"/>
    <dbReference type="HOGENOM" id="CLU_050910_3_0_1"/>
    <dbReference type="OrthoDB" id="47276at2759"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043527">
        <property type="term" value="C:tRNA methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine(46)-N7)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03055">
        <property type="entry name" value="tRNA_methyltr_TrmB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025763">
        <property type="entry name" value="Trm8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00091">
        <property type="entry name" value="tRNA (guanosine(46)-N7)-methyltransferase TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417:SF16">
        <property type="entry name" value="TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="200"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="223" mass="25776" checksum="55C61A3AD2A048F9" modified="2012-10-03" version="1">MQLKNIKKGLYRQRAHANPFKDSNIVIPPNPQSVDWASYFEINRRPDFVDIGCGYGKFLMKVAEKNPGRNVLGLEIRDKVCAYVRANIENLRIPNAGVMRTNALIFLPNIFSGGQLSKIFILFPDPHFKKRKQKGRIVCQQMMEVYEYLLRNEGRLYISTDVEDLFDYMVDVILAHKGFEPLSEEEMNKDPLFYMISKDTDEALRAGVKTGKVFSKVFEIKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUI9</accession>
    <name>I7AUI9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_111460</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265624.1">
        <property type="nucleotide sequence ID" value="XM_009267349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUI9"/>
    <dbReference type="GeneID" id="20564745"/>
    <dbReference type="KEGG" id="ero:EROM_111460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111460"/>
    <dbReference type="HOGENOM" id="CLU_078296_0_0_1"/>
    <dbReference type="OrthoDB" id="10253329at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008913">
        <property type="entry name" value="Znf_CHY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037274">
        <property type="entry name" value="Znf_CHY_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05495">
        <property type="entry name" value="zf-CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161219">
        <property type="entry name" value="CHY zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51266">
        <property type="entry name" value="ZF_CHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CHY-type" evidence="6">
        <location>
            <begin position="248"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="344" mass="39534" checksum="504B11AC1B1F1A49" modified="2012-10-03" version="1">MVDLNTNKVVRKGDNILVYLNQPKDFIYDIDNIAVEYSEVGKSVEVVNDQIPKFIKDNMKRFFRGDLKEYVGFLEENLEIFFKGEVPETERKEQTKRSFELPSDYKFPINKRVQMNVAVEVEKRYTSIVSCECLNLQAGCNRCGRILEMPGPTECPGCKCRVEINYIPSVDSEFLGFLGLHGCKLICFNPSRYQLSCDSCHMNYETSELGIGDTFRIKCYECLSNISLKISNIKLIQKKKETLKPGQPLPDKGVCRHYKKSYRWFRFPCCNSLYPCDICHDEESGHVHQMANKMVCGLCSKEQGVSKACDCGMNLKKSTSFWEGGKGSRNKATMSRKDRKKYTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHJ8</accession>
    <name>I6ZHJ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphatidylinositol-4-phosphate-5-kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009265865.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHJ8"/>
    <dbReference type="GeneID" id="20520932"/>
    <dbReference type="KEGG" id="ero:EROM_030230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030230"/>
    <dbReference type="HOGENOM" id="CLU_045584_0_0_1"/>
    <dbReference type="OrthoDB" id="20783at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016308">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00139">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023610">
        <property type="entry name" value="PInositol-4/5-P-5/4-kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086:SF8">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 5-PHOSPHATE 4-KINASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23086">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PIPK" evidence="2">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="35035" checksum="9C587FF8DBD69861" modified="2012-10-03" version="1">MENQKINLMLESLSQIEDMMISKPSTSDVEEMRKENEVLVKTYNYKEFREIRALNGTEGLHDLGRQYVLNEQIHGKSGSFLFFTEDLKFVVKTIRKSEFYCIRRMVEEYKIHILQNPMSLLCKILGCYSLCTRGDEEYFIVMESMLKRSGMQEIYDLKGASVKRKGYSVSSLKEIDWIGNSKRIDLGNRKEAIISQIKNDVEFLKRHRIMDYSFFVSFGWKKRESVDPFSLCKDHNHMPENEDVHFGIVDILTQWTFKKRMERLFHIFCCKSNSSCLNPDAYMDRFLIMIETDVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHN8</accession>
    <name>I6ZHN8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional G2/M transition repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_030820</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264200.1">
        <property type="nucleotide sequence ID" value="XM_009265925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHN8"/>
    <dbReference type="GeneID" id="20520993"/>
    <dbReference type="KEGG" id="ero:EROM_030820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030820"/>
    <dbReference type="HOGENOM" id="CLU_1390216_0_0_1"/>
    <dbReference type="OrthoDB" id="3269380at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051580">
        <property type="entry name" value="ZnF-Chromatin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23057:SF0">
        <property type="entry name" value="JUXTAPOSED WITH ANOTHER ZINC FINGER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="132"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="168"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="23615" checksum="0823E1208CEE2FDD" modified="2012-10-03" version="1">MMRTNDSSKLKKLYQIEEKRFEERVKRYEDIGYMMDMHQFYLEDVLLKREEMLYKYTLPEKDAPELSEEDNPGFDYEFIHKSVGDREWRNGIPNSGMIKVRGLPVSTKTTYREEKTTRSHSKAQRTKGGRVYACEIEGCTKKYTSSFGLKYHMKEGHSEEKMNIFKPYVCPFDGCDKKYKNNNGLKYHIKHYHDDQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHT1</accession>
    <name>I6ZHT1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264255.1">
        <property type="nucleotide sequence ID" value="XM_009265980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHT1"/>
    <dbReference type="GeneID" id="20521050"/>
    <dbReference type="KEGG" id="ero:EROM_031360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031360"/>
    <dbReference type="HOGENOM" id="CLU_176429_0_0_1"/>
    <dbReference type="OrthoDB" id="2191610at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="107" mass="12000" checksum="4790F87CB0E20C6F" modified="2012-10-03" version="1">MEDQCHILPFFIPETSKVKIEYVDLSGDVSTIRGIFCVKKKLDTDINVHICNKIVKDPIFYWVPANSESVLEKGLELVRKGLGFPLKGDQGPPSKTITKKNNKGVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHT8</accession>
    <name>I6ZHT8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ca2+-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_031460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264265.1">
        <property type="nucleotide sequence ID" value="XM_009265990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHT8"/>
    <dbReference type="GeneID" id="20521060"/>
    <dbReference type="KEGG" id="ero:EROM_031460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031460"/>
    <dbReference type="HOGENOM" id="CLU_061288_9_3_1"/>
    <dbReference type="OrthoDB" id="429467at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000003">
        <property type="entry name" value="Calmodulin A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="88"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="159" mass="18130" checksum="556A94BEADF4D3E9" modified="2012-10-03" version="1">MRRRQRRQSSNIFHMLTKNQIVELREAFNFMDVNSDTFVDRGDLESFLASIGSPFSSEEISEMMAESGDNMTYMLFLTMIGERLSCTDPEKDIFNALKEFDDNGDGTIDEKVLRAWLMEKGDQMAGEDVDLLLKGCVENGRVDCKSLTTKIKYGEIIAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI58</accession>
    <name>I6ZI58_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264405.1">
        <property type="nucleotide sequence ID" value="XM_009266130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI58"/>
    <dbReference type="GeneID" id="20521206"/>
    <dbReference type="KEGG" id="ero:EROM_041430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041430"/>
    <dbReference type="HOGENOM" id="CLU_031976_0_0_1"/>
    <dbReference type="OrthoDB" id="2192975at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="598" mass="66477" checksum="713059D0894D5CB8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIA0</accession>
    <name>I6ZIA0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD40 domain-containing periodic tryptophan protein 2-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264445.1">
        <property type="nucleotide sequence ID" value="XM_009266170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIA0"/>
    <dbReference type="GeneID" id="20521247"/>
    <dbReference type="KEGG" id="ero:EROM_050150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050150"/>
    <dbReference type="HOGENOM" id="CLU_022997_0_0_1"/>
    <dbReference type="OrthoDB" id="3142434at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027145">
        <property type="entry name" value="PWP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19858">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="405"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="727" mass="83387" checksum="B21B23793993CBB9" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIB5</accession>
    <name>I6ZIB5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase alpha/epsilon subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264460.1">
        <property type="nucleotide sequence ID" value="XM_009266185.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIB5"/>
    <dbReference type="GeneID" id="20521262"/>
    <dbReference type="KEGG" id="ero:EROM_050300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050300"/>
    <dbReference type="HOGENOM" id="CLU_687157_0_0_1"/>
    <dbReference type="OrthoDB" id="2196015at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016266">
        <property type="entry name" value="POLE2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12708">
        <property type="entry name" value="DNA POLYMERASE EPSILON SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="397" mass="45475" checksum="811F17745262F354" modified="2012-10-03" version="1">MRERIYKEFTCERGIMLSSDALVYLEDNVKSEAMLKAISSEYRRRNGQRLADINSIREVVESFDAKKDTYNILNQEFKEKNELLKYKHLLEKIDRIVTPIYLLGEECATIFGCYYKDREGREVLEDDGDVIPLDMSKCNEDVFLCDNIFIAIEGKKTDGKFVGLKVYLPSVEKKVGSLSKLIKKDLKILFFSDFQMNEVNGRTLRKILGKMPADIVVLMGRLYPGISSTIPCISFMDGLRPNNAQTETPDIILCPDADDTYPSFLPKEIEVPREYGKFIKAASNPFILETYGYSIGVIREDIFRYKEQGTFIGKNYVDSFAREILSQHSFNPFGISNLDIDRVPDMFIVGQDFYPFITSVEDTMFISCPSFKDEMAFVSYETSSGDPIILSTRHLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIE5</accession>
    <name>I6ZIE5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L10A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_050610</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264490.1">
        <property type="nucleotide sequence ID" value="XM_009266215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIE5"/>
    <dbReference type="GeneID" id="20521293"/>
    <dbReference type="KEGG" id="ero:EROM_050610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050610"/>
    <dbReference type="HOGENOM" id="CLU_062853_3_1_1"/>
    <dbReference type="OrthoDB" id="2449818at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="218" mass="24872" checksum="C9B2B89944EC9482" modified="2012-10-03" version="1">MNNQSEEQQLAFLDPKRITSIIDRLKENEDPEKTATIQIQVNLKGYDPRKDNKLSKDMSFPYKVRRLDKIIVIADEAHIKACVDAGLPHVSIEEIAGDDKKAKRDAVLKENKYFILCSGYNKVFQLKNILRFGKTPYILKDDDDIKAVFEAAKRSYKLRIKDFAVTSFPVGHTGMDSDHIYENIKTGMGLLISYLKKGTQNLKGVMIKTNQQSPVTLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIK5</accession>
    <name>I6ZIK5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein YOP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_051220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264550.1">
        <property type="nucleotide sequence ID" value="XM_009266275.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIK5"/>
    <dbReference type="GeneID" id="20521354"/>
    <dbReference type="KEGG" id="ero:EROM_051220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051220"/>
    <dbReference type="HOGENOM" id="CLU_115042_1_0_1"/>
    <dbReference type="OrthoDB" id="434647at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300">
        <property type="entry name" value="HVA22-LIKE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12300:SF161">
        <property type="entry name" value="RECEPTOR EXPRESSION-ENHANCING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="142"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="22555" checksum="3EA211729A1736D2" modified="2012-10-03" version="1">MIKLLINAFVAMLVLYNVLQAFKACREDKRIYRRYNKYFMVIAVFLVLDNLFSFVLDFIPFYQLFKLMVVAWISIPACTGAVFVYKFYIHGFMDKYQGALEEMVEKARSAVFRYFNECYDKAQKTYHGRKISEVEEEALGIKNEGEGSKISPEDSNMEDVSDSGFSTIDSTVINGEKQSSNIDVKMPGKPVDDTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIP7</accession>
    <name>I6ZIP7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264595.1">
        <property type="nucleotide sequence ID" value="XM_009266320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIP7"/>
    <dbReference type="GeneID" id="20521400"/>
    <dbReference type="KEGG" id="ero:EROM_060030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060030"/>
    <dbReference type="HOGENOM" id="CLU_637927_0_0_1"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16450">
        <property type="entry name" value="mRING-C3HGC3_RFWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037381">
        <property type="entry name" value="RFWD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16047:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16047">
        <property type="entry name" value="RFWD3 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="418" mass="47901" checksum="BC456FF709991666" modified="2012-10-03" version="1">MDCGTICPICFSEYTTKGNHRVVSLQCGHLFGSQCIQKWVGKKSKMQCPLCSVQSTKRQIRPVYASKIVAIDTENEQKLLERCLREEKEKNEYIEICTGLKAQIEALKMELLHAKEEKTENTFLIHKHKKFSINAGFNTANSIIWYDESNCTMIVTRKSGKNVGIQKFESYDFSKSEFIVLGEGPPIKNMSLSPFNEDLGLLPIGNVLNIVNIYNGNVVAKYIAHNQIESTCFDKDDRNTIYCGDDKGSVYFINISSCEPLKILKVSDISIHSICKKGLEVFASTIYQTYRIIFSGDCIPCNLEMEPYSICTNMSAYGNHLLLTFRSLDLRVKHFIYGKKEVCLSLGIKQAKKHRDRIHRDHIYIVDDERSSIRVLKLHSLEVIYTYTFKERVLDFFVCDTFLFVLAKFAIHIFSNNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ21</accession>
    <name>I6ZJ21_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264705.1">
        <property type="nucleotide sequence ID" value="XM_009266430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ21"/>
    <dbReference type="GeneID" id="20521512"/>
    <dbReference type="KEGG" id="ero:EROM_061160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061160"/>
    <dbReference type="HOGENOM" id="CLU_1758791_0_0_1"/>
    <dbReference type="OrthoDB" id="2193243at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="142" mass="16245" checksum="0792042CE067DBEC" modified="2012-10-03" version="1">MLLELEKIKEVSIPHLYVFPTGSLEFTCAMFKGSGYTTFKIVKYKIYEKWKVLLDEECNKEPSAIPSACDAYTTLQEIVKVLRSYSNAKIFEKPKGVFPFISKGPIDRKRNISALLLFIENKCLKKNSNGQKSAIQLKEEVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ25</accession>
    <name>I6ZJ25_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264710.1">
        <property type="nucleotide sequence ID" value="XM_009266435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ25"/>
    <dbReference type="GeneID" id="20521517"/>
    <dbReference type="KEGG" id="ero:EROM_061210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061210"/>
    <dbReference type="HOGENOM" id="CLU_672737_0_0_1"/>
    <dbReference type="OrthoDB" id="2191179at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="409" mass="49108" checksum="C9ADF3E6EB3B3803" modified="2012-10-03" version="1">MIFCETCGSPLEIIGNDYVCSEGHITKNTIEFSNEERPILLDSSTNDTKLKKLLFKKYGPDYMELLGFYILFNDIREHLCIKSLKYFNIFANSIKEGPKKTLPRFLLVSPTLRALTYYSKRIEMEAEGKTYLYMDYYKSMMTYPYEERRRIKLTHLGITNLNIPPVRFEGTGLIPVYRILRFLGERNVRLQKNRSHGRLVIFYGETGVLDEKAENNKACFREDLRRDYEMFFKYLQRICDIFVLEITEDLEEKFKAFFYMLDLTKTIHIPEVEISVFLYIYIMNYKKDFDVLRAIRNLNEILREPYSTLPEEIKYIKPEDLYGKEERLQSYLKTLISKATWFNSTEIEEKLGNFKRLFSSSRTQRLFNDYWGRIHDRHKPLFIRNAWYVKKLIILKRMKTMKESEEDHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ80</accession>
    <name>I6ZJ80_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Vesicular transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264785.1">
        <property type="nucleotide sequence ID" value="XM_009266510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ80"/>
    <dbReference type="GeneID" id="20521596"/>
    <dbReference type="KEGG" id="ero:EROM_070370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070370"/>
    <dbReference type="HOGENOM" id="CLU_079401_0_0_1"/>
    <dbReference type="OrthoDB" id="2196332at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="297" mass="33598" checksum="95BFE5FA6A4FA9C0" modified="2012-10-03" version="1">MESLERSSTGSEKFSGNILDTDSSITILDIPCSISNTIQPKTSSEVALQSSFPESIDPAFADYYFVFPQTLESFVGRIKDKRFVYRFSENKALEIVRCKDHETVYALDVFDLEKDDIGACNNSISLVVNRSKYRSSVKAKEDREYRKALSEGRRYIRGRSPYESKIEAIHLEQITNSRNFVFADDPQDLPGELKAIIKHLHSKRVHVPKTKAFSVEQKTDHLQNVLQNIPGIGKDAARSLSLHFKSISRLYSFLKGENNGQLSELKVWDSDGKHSRPLGEKQSDKIRNAFIGKSGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJB0</accession>
    <name>I6ZJB0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nuclear protein localization protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_070720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264820.1">
        <property type="nucleotide sequence ID" value="XM_009266545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJB0"/>
    <dbReference type="GeneID" id="20521631"/>
    <dbReference type="KEGG" id="ero:EROM_070720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070720"/>
    <dbReference type="HOGENOM" id="CLU_017172_0_0_1"/>
    <dbReference type="OrthoDB" id="10251089at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08061">
        <property type="entry name" value="MPN_NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016563">
        <property type="entry name" value="Npl4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007717">
        <property type="entry name" value="NPL4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007716">
        <property type="entry name" value="NPL4_Zn-bd_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036443">
        <property type="entry name" value="Znf_RanBP2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12710:SF0">
        <property type="entry name" value="NUCLEAR PROTEIN LOCALIZATION PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05021">
        <property type="entry name" value="NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05020">
        <property type="entry name" value="zf-NPL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010052">
        <property type="entry name" value="Polyub_prc_Npl4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90209">
        <property type="entry name" value="Ran binding protein zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01358">
        <property type="entry name" value="ZF_RANBP2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RanBP2-type" evidence="8">
        <location>
            <begin position="477"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="505" mass="58289" checksum="1CBC48F0BBE34E67" modified="2012-10-03" version="1">MIFIVRGPEGQRRVEVDEARSLGPQLLAFFEVDQISVFSTPDKQDEFDKSQTPVTLGLKNGQVLYVEYEKKNEMPKKRQDGDKTIKRGRDRMLCQHDSNAMCSNCAPLDPWDEKYYKDNMIKYLSFGSYHEMMKSKKKELYVDDYSVVVCSDHGSNTRCNRCQEKNIILTPQVFRMVDHVEFDGQHLVENFIRNWRESGRQRFGFLVGRYMDHDMIPLGMKAVVSGIWEPEQEDYPDGFVITGSIDEPFGGTGFGIVGMIYTDILMEKGGVTSDKLIRDYFLSSLEIEFMAKMQLMHPHTLKDGGHEVEFGSRFVTIIATVEKDGSIGLQEYQVSNQCMALVKGNWILPTENPKVFLARRDILYRTKAKESMIKADPYLPGEFFLVKLTHGCKANPLFKNTEFIPKKFGDRRMAEYFGGDFSLEKFSNFTLLTRMRGMFSNWKDLFICISSQNRSKFKAISKSKDFKSFVSSMERYRCAGWECRACTFVNERNTSACEMCNGKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJS9</accession>
    <name>I6ZJS9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">cAMP-dependent protein kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265005.1">
        <property type="nucleotide sequence ID" value="XM_009266730.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJS9"/>
    <dbReference type="GeneID" id="20521825"/>
    <dbReference type="KEGG" id="ero:EROM_080900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080900"/>
    <dbReference type="HOGENOM" id="CLU_075121_0_0_1"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF152">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE I REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="117"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="233" mass="26664" checksum="0B7A6DBB7B450881" modified="2012-10-03" version="1">MSRDNEAFKKIEDKLKELKFSVNEDVVQSIYEMASKEDGEGDPGKLIEMLVEDGTLMRLQLNSKESDKKRLNEEWRASRRSGVYSERVTPETFQLRYYPKNEETMKFLSDILVGDIPFGFLNPDQKIRLIESMELIEIKCGTFVMHEEDVGSQMYIVESGEFEVTRGGCFLRKLSRGSFFGEIALLHNIPRTATVKAISDGKVWVVEQTSFSGIRMNGQDPKQEDHSGGSERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJW5</accession>
    <name>I6ZJW5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265050.1">
        <property type="nucleotide sequence ID" value="XM_009266775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJW5"/>
    <dbReference type="GeneID" id="20521872"/>
    <dbReference type="KEGG" id="ero:EROM_081370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081370"/>
    <dbReference type="HOGENOM" id="CLU_097995_0_0_1"/>
    <dbReference type="OrthoDB" id="2195369at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031523">
        <property type="entry name" value="Acetyltransf_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17013">
        <property type="entry name" value="Acetyltransf_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="29104" checksum="7CD613069970548A" modified="2012-10-03" version="1">MIIALFIYPFVLGMETCRFLKYFSDEQGIKMAEQIVDDYNEAEGEFGTKAMTFDHIFHGGSRMDIFQYKIGEELVASVFLCRTTFYGMVDGNIVNRSAVFVYTMYVSKKHRGRGYSKRILRDSTDSLNSHYGMNGDFLLVLHLNPRDKSMELAFSIYYNLNFRNGGLTMIGPGEKQYFLEELLDYRNPCDVIDEYDEERDRGMYMVMFCEYSKLFICEKERYSKLMSYGSRLRSILQKGRLQESSSSML</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK88</accession>
    <name>I6ZK88_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">S1-like RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_090610</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265175.1">
        <property type="nucleotide sequence ID" value="XM_009266900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK88"/>
    <dbReference type="GeneID" id="20564285"/>
    <dbReference type="KEGG" id="ero:EROM_090610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090610"/>
    <dbReference type="HOGENOM" id="CLU_069847_2_0_1"/>
    <dbReference type="OrthoDB" id="340500at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21262">
        <property type="entry name" value="RRP40_S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0271">Exosome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="129"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="194" mass="21488" checksum="DEDA9815A8F24B22" modified="2012-10-03" version="1">MLTKIRNVLPGDEVSSNDSKSLGIHENKAMIVGTLCRASRHLFVISKTNRYHPCIDDIVIGKAILTSQDIHRMDLGGMVGVLPSLSFTNATKRNKPEISKGDYLLCKIARMGIEPLLTCLGEGFGKLNGVVLPLDPWKVRLLYLSTALSTIGKKYKFRIALGLNGFVWIEAEKDTDIRDIYHLLHEDPQASLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKM9</accession>
    <name>I6ZKM9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_100470</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265360.1">
        <property type="nucleotide sequence ID" value="XM_009267085.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKM9"/>
    <dbReference type="GeneID" id="20564477"/>
    <dbReference type="KEGG" id="ero:EROM_100470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100470"/>
    <dbReference type="HOGENOM" id="CLU_012393_2_0_1"/>
    <dbReference type="OrthoDB" id="1751331at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="11">
        <location>
            <begin position="177"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="14">
        <location>
            <begin position="292"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="13">
        <location>
            <begin position="421"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="117"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="573" mass="64887" checksum="FEFBB422E7E261F9" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKZ1</accession>
    <name>I6ZKZ1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disease resistance R13L4/SHOC-2-like LRR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265485.1">
        <property type="nucleotide sequence ID" value="XM_009267210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKZ1"/>
    <dbReference type="GeneID" id="20564603"/>
    <dbReference type="KEGG" id="ero:EROM_110040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110040"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_15_1"/>
    <dbReference type="OrthoDB" id="2192374at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="95"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="31001" checksum="05A75930DAF619D1" modified="2012-10-03" version="1">MIITKLIIGGFVRCSFDSSSSTLNDSNYWSKEVDYSNQGLTEFPEEIFEISSTSCVVEVNIQKNKICSIPSKIESLKKLLRLYMNDNQIQSLPPEIGSVERLRELDVSNNNISWLPVEITHLQWLTKLNVSNNSLRRLPVEMRVLPFLKELRADGNKLCALPSTFEEFYVLERISLGNNLFEEIPEVIGKMENLKKIDFRNNRIRRVNRKLVDILFDNRNLELIDLRGNDLMRESDSEWVGWEELGRMFRSDILLSQLSRPGIDDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL47</accession>
    <name>I6ZL47_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein transport protein SEC23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_110660</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Cytoplasmic vesicle</location>
            <location evidence="1 12">COPII-coated vesicle membrane</location>
            <topology evidence="1 12">Peripheral membrane protein</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 12">Endoplasmic reticulum membrane</location>
            <topology evidence="2 12">Peripheral membrane protein</topology>
            <orientation evidence="2 12">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus membrane</location>
            <topology evidence="12">Peripheral membrane protein</topology>
            <orientation evidence="12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the SEC23/SEC24 family. SEC23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265545.1">
        <property type="nucleotide sequence ID" value="XM_009267270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL47"/>
    <dbReference type="GeneID" id="20564665"/>
    <dbReference type="KEGG" id="ero:EROM_110660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110660"/>
    <dbReference type="HOGENOM" id="CLU_008658_3_0_1"/>
    <dbReference type="OrthoDB" id="10256289at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1670">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.730">
        <property type="entry name" value="Sec23/Sec24 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.380">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012990">
        <property type="entry name" value="Beta-sandwich_Sec23_24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036180">
        <property type="entry name" value="Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037364">
        <property type="entry name" value="Sec23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006900">
        <property type="entry name" value="Sec23/24_helical_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036175">
        <property type="entry name" value="Sec23/24_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006896">
        <property type="entry name" value="Sec23/24_trunk_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006895">
        <property type="entry name" value="Znf_Sec23_Sec24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036174">
        <property type="entry name" value="Znf_Sec23_Sec24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11141:SF0">
        <property type="entry name" value="PROTEIN TRANSPORT PROTEIN SEC23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08033">
        <property type="entry name" value="Sec23_BS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04815">
        <property type="entry name" value="Sec23_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04811">
        <property type="entry name" value="Sec23_trunk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04810">
        <property type="entry name" value="zf-Sec23_Sec24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81995">
        <property type="entry name" value="beta-sandwich domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82754">
        <property type="entry name" value="C-terminal, gelsolin-like domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81811">
        <property type="entry name" value="Helical domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82919">
        <property type="entry name" value="Zn-finger domain of Sec23/24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zinc finger Sec23/Sec24-type" evidence="13">
        <location>
            <begin position="50"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 trunk" evidence="14">
        <location>
            <begin position="118"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 beta-sandwich" evidence="16">
        <location>
            <begin position="379"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Sec23/Sec24 helical" evidence="15">
        <location>
            <begin position="473"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU365030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AFN84048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="707" mass="80470" checksum="B63D598604BA7C27" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLE9</accession>
    <name>I6ZLE9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_111520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265630.1">
        <property type="nucleotide sequence ID" value="XM_009267355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLE9"/>
    <dbReference type="GeneID" id="20564751"/>
    <dbReference type="KEGG" id="ero:EROM_111520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111520"/>
    <dbReference type="HOGENOM" id="CLU_108567_0_0_1"/>
    <dbReference type="OrthoDB" id="2194145at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="222" mass="25527" checksum="CEE02CF55D7E50CE" modified="2012-10-03" version="1">MQPLYFISTKFCVVPHVIDAQSVPSTDKNVKIYIDDILYKDSAYEGGLDLEIKEFCALSNIKTCLGFRNKPNTLVSKRGHYKLKRQKYEEILEVLKPDYYADFDTGKIFIDGNELIEPKEVEEFIELLNKGHLVFGTEFINNLVNQGMMMKFDGCRLDVCGIFDCKCCGDLSAGYLKYLWSIKEMNALTYLAIHNYNVLDEVFEAIGSNKISYREVEMGDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS51</accession>
    <name>I6ZS51_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263923.1">
        <property type="nucleotide sequence ID" value="XM_009265648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS51"/>
    <dbReference type="GeneID" id="20520709"/>
    <dbReference type="KEGG" id="ero:EROM_010820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010820"/>
    <dbReference type="HOGENOM" id="CLU_1069978_0_0_1"/>
    <dbReference type="OrthoDB" id="2189952at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="261" mass="29701" checksum="EF23CAA441CBE66D" modified="2012-10-03" version="1">MESPGCLRRQAMLYSIPLLLVASHFPGPYLFVLSIPSSLIAFLVPGTKRRMYISNSLFILSLLFPELYFLCYGFTMAAIYVSVSNLCMDRFRRENLVIAGMMESVGMLLSLYSLKNNVVSMFLAVFHFLIIHRLMSPPIESDDYGQAYKKIAWLLREASFTDRANEYREVVGCRGIVIPEFRIDLWTPLLMGRLSCLSKAIICCSSLIPIGKSHWMGFVVYSLSFLPVEWACAGLSFFCDFTPGHPFYRLVSAQVLYLITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSC1</accession>
    <name>I6ZSC1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">M2 dsRNA satellite propagation-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264003.1">
        <property type="nucleotide sequence ID" value="XM_009265728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSC1"/>
    <dbReference type="GeneID" id="20520789"/>
    <dbReference type="KEGG" id="ero:EROM_020310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020310"/>
    <dbReference type="HOGENOM" id="CLU_533267_0_0_1"/>
    <dbReference type="OrthoDB" id="17262at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG-I" evidence="1">
        <location>
            <begin position="139"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="526" mass="60226" checksum="870AEB29E795CBD8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSJ8</accession>
    <name>I6ZSJ8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="11">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_021060</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="11"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264078.1">
        <property type="nucleotide sequence ID" value="XM_009265803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSJ8"/>
    <dbReference type="GeneID" id="20520868"/>
    <dbReference type="KEGG" id="ero:EROM_021060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021060"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000501">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="12">
        <location>
            <begin position="319"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="709" mass="79538" checksum="3AFA385821C5E656" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSW0</accession>
    <name>I6ZSW0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264168.1">
        <property type="nucleotide sequence ID" value="XM_009265893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSW0"/>
    <dbReference type="GeneID" id="20520961"/>
    <dbReference type="KEGG" id="ero:EROM_030500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030500"/>
    <dbReference type="HOGENOM" id="CLU_128308_1_0_1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="5">
        <location>
            <begin position="19"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="3">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="107" mass="12493" checksum="50C582F334BEE2FD" modified="2012-10-03" version="1">MEAQAVLGMILLFHPERCLKRPGEKVRKSKFQKEVLRKIYQITPYPTWENKIDIGILISLSPRAVDIWFQNKRHINKGKNQGAEDTTDSRTIDLKEIVSIVENTPRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUA4</accession>
    <name>I6ZUA4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulation complex subunit NOT5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_061500</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264738.1">
        <property type="nucleotide sequence ID" value="XM_009266463.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUA4"/>
    <dbReference type="GeneID" id="20521547"/>
    <dbReference type="KEGG" id="ero:EROM_061500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061500"/>
    <dbReference type="HOGENOM" id="CLU_102322_2_0_1"/>
    <dbReference type="OrthoDB" id="293823at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="97"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="227" mass="27131" checksum="7B94C1BC52BD8D2D" modified="2012-10-03" version="1">MKKPQDSKKDESRRDKNVKSPLLKEKLTPEEVWKNAAILLGTKKKETVPKSTQGIKSFDFGDVCIEIEKVNRERISLHGSGHEDGVRRFYEIETSNSLVHIPNFRDLEKNYTPRTPVETPSFFPKVALYVFESQDIFKKLDIDTLFFIFYSQLGTIQQYYAAVQLKTYSWRFHTKYFTWFQRLDEPKLITADYERGDFLFFDYDVTWSFMKKSDFTFEYKYLECSEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUN4</accession>
    <name>I6ZUN4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication complex GINS protein SLD5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The GINS complex plays an essential role in the initiation of DNA replication.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GINS4/SLD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264918.1">
        <property type="nucleotide sequence ID" value="XM_009266643.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUN4"/>
    <dbReference type="GeneID" id="20521734"/>
    <dbReference type="KEGG" id="ero:EROM_071700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071700"/>
    <dbReference type="HOGENOM" id="CLU_127203_0_0_1"/>
    <dbReference type="OrthoDB" id="338231at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008591">
        <property type="entry name" value="GINS_Sld5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206:SF0">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21206">
        <property type="entry name" value="SLD5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007764">
        <property type="entry name" value="Sld5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="19800" checksum="98391EDD8F6415AA" modified="2012-10-03" version="1">MPLPTLNDLILAYQNEKSAKRLLPYASCPMKHFLDVVSRRAGDMKALGKTIVRNVYELELERVKFFIKEYIRVRLKKLSTNLYVDRSFLSERETIFYEKYIGLLKERDIYVPEQKFCKKNEFIGFYCCVDVDGIMIDGELLEVFKGDFFVAPLSDVMDLLKRNEIILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUW5</accession>
    <name>I6ZUW5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNase P subunit p30-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_081200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265033.1">
        <property type="nucleotide sequence ID" value="XM_009266758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUW5"/>
    <dbReference type="GeneID" id="20521855"/>
    <dbReference type="KEGG" id="ero:EROM_081200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081200"/>
    <dbReference type="HOGENOM" id="CLU_100281_0_0_1"/>
    <dbReference type="OrthoDB" id="2194280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13031">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="238" mass="27933" checksum="03BBC06E39C82FF0" modified="2012-10-03" version="1">MYYDVNISLDYLKQDMKELEENEYEGFCVNRIIKPRDLEKIRYEPVEFLGAKPCYSRVEVVFEEKEEVGYDAKKSMKYDLFVVRLNGVAGMDRLIRCQPDMITFNYGSQILPFKCGLVRTAIKENIFFEVPLRESLYGGTSSIMWMRNVRKLLFITNGKNVVFSSGARCSTEIKRPRDITKMLEMFGLKKKRASEVMLNSLRLLRRCAMKRYYYRGSITHSVDEGSLKRDFVLSLYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUW9</accession>
    <name>I6ZUW9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265038.1">
        <property type="nucleotide sequence ID" value="XM_009266763.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUW9"/>
    <dbReference type="GeneID" id="20521860"/>
    <dbReference type="KEGG" id="ero:EROM_081250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081250"/>
    <dbReference type="HOGENOM" id="CLU_652161_0_0_1"/>
    <dbReference type="OrthoDB" id="2192449at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="421" mass="48552" checksum="3BD38E39CB9FBB6C" modified="2012-10-03" version="1">MHLKTMTKSLEGYKEVKVIRREQRILISSAVDMTSGEKIILESVDLSGFSEDYRSLLMSFLLNHKINHSPYAMKVLKVLEGEDWICVVQKHMSLGPVDGLIRDQIFLSDDFIFYVFMQIVYAMKINGDEGWFWDKMSPRYIWLDEDGKVRICWADVVLGHMKALGEQIERWRKGTECGKDVRLQSKIDGESVSFRNLRKIFVELALNPSEPSRMIYTKDMEDLEDSPLAGHKEKLSPALNGFASLLFEEKGKVSDLEEYTRRMIQEKKIVSNPREIFKPLRMIIEERNRDEEFSTLEGMKEHSGGILKMEEGESASGSEEGWKSESGSKGDDERVGSFGTREYKRGRFHVCEAIPLGEREKGLKMNEQMKRMVRIVSVQNQQISLLTKVLKQSGLLTKEVDGELCELEELMSFLLFGMERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVB1</accession>
    <name>I6ZVB1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">DNA polymerase</fullName>
            <ecNumber evidence="20">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">EROM_090440</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="20"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265158.1">
        <property type="nucleotide sequence ID" value="XM_009266883.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVB1"/>
    <dbReference type="GeneID" id="20521983"/>
    <dbReference type="KEGG" id="ero:EROM_090440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090440"/>
    <dbReference type="HOGENOM" id="CLU_000203_2_1_1"/>
    <dbReference type="OrthoDB" id="2414538at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008296">
        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05777">
        <property type="entry name" value="DNA_polB_delta_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05533">
        <property type="entry name" value="POLBc_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000001">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000004">
        <property type="entry name" value="DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
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        <property type="entry name" value="Znf-C4pol"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14260">
        <property type="entry name" value="zf-C4pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 20" id="KW-0235">DNA replication</keyword>
    <keyword evidence="17 20" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14 20" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="13" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 20" id="KW-0408">Iron</keyword>
    <keyword evidence="16 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
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    <keyword evidence="18 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="22">
        <location>
            <begin position="117"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="21">
        <location>
            <begin position="408"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="domain" description="C4-type zinc-finger of DNA polymerase delta" evidence="23">
        <location>
            <begin position="870"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF14260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AFN83661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="959" mass="109379" checksum="2B053F775C92CB00" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVE7</accession>
    <name>I6ZVE7_ENCRO</name>
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        <submittedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090850</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266923.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVE7"/>
    <dbReference type="GeneID" id="20564309"/>
    <dbReference type="KEGG" id="ero:EROM_090850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090850"/>
    <dbReference type="HOGENOM" id="CLU_041217_10_1_1"/>
    <dbReference type="OrthoDB" id="9989112at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
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        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="203" mass="23331" checksum="75AF58B8452E82E2" modified="2012-10-03" version="1">MVAPRIDYRYKIIVLGESDVGKTCLLHKYKEDDFKQSLMTTIGVDTVSKSIVLNGKNIMLNIWDTAGQERFFSITKSYYRNADGILLIFDISDERTFSCVDRWFGRIREETGNVPLFLVGNKRDKVDSATYHAMEGNFKSKAEEMKARYYTTSAKTGENVNNIFEDMAMVLLKKQVLGQKWESGNLYNNPVNEVNRKTNSRCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVG3</accession>
    <name>I6ZVG3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265218.1">
        <property type="nucleotide sequence ID" value="XM_009266943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVG3"/>
    <dbReference type="GeneID" id="20564329"/>
    <dbReference type="KEGG" id="ero:EROM_091050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091050"/>
    <dbReference type="HOGENOM" id="CLU_1660740_0_0_1"/>
    <dbReference type="OrthoDB" id="2193650at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17194" checksum="2379D3F6FB0E678E" modified="2012-10-03" version="1">MKTVAVVMRSLDDEIPSLRVPAASALLGSAKHIVIIFIEKNKMFLFNEPHVMLESEKILSVEQKLHRKAIVTHLENTAKAVKKINFKAEVSKLVISGDEKYKIKKCLQCLEVQAAMFVGRHKRKKFYEYFVQSLEDYVFEIMKIPTIRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVH5</accession>
    <name>I6ZVH5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265233.1">
        <property type="nucleotide sequence ID" value="XM_009266958.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVH5"/>
    <dbReference type="GeneID" id="20564344"/>
    <dbReference type="KEGG" id="ero:EROM_091200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091200"/>
    <dbReference type="HOGENOM" id="CLU_121524_0_0_1"/>
    <dbReference type="OrthoDB" id="367221at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2300.10">
        <property type="entry name" value="THUMP superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040183">
        <property type="entry name" value="THUMPD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452">
        <property type="entry name" value="THUMP DOMAIN CONTAINING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13452:SF10">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="189" mass="22093" checksum="C7CBB54E695255E3" modified="2012-10-03" version="1">MRTGFFVSCRLNKEPKGLREVIGRLQGLVEPPTKDVRGRSIRQILEEEIEEYKSSTKFAKHDSYRCILFLENRSAETSLSLFYKLRASKTRFEYVYRVVPLDNFFQFDEEKILESVRGLDESKSYKIVYEGRLCHADMKERVFNLITKNISLRVDLESPHYIIVTQAFKNSLGISVVEGEKENFNFSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVZ4</accession>
    <name>I6ZVZ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dullard-like phosphatase domain-containingprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265428.1">
        <property type="nucleotide sequence ID" value="XM_009267153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVZ4"/>
    <dbReference type="GeneID" id="20564546"/>
    <dbReference type="KEGG" id="ero:EROM_101160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101160"/>
    <dbReference type="HOGENOM" id="CLU_020262_4_5_1"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000093">
        <property type="entry name" value="NLI interacting factor-like phosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="46"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="229" mass="26661" checksum="8498E56BE543AC67" modified="2012-10-03" version="1">MSMPWILKILRGLCGCYKKEVSMESRRKMKKVNCDEESPRSILPRKYDPKPTLVLDLDNTLVHSTFEMPEFYDFCVEVPRSRDMPIYVKVRPHATEFINGVGDIYEVIIFTAAKKEYARKVIDMIDMNKNIKYSLYRESCTLTNGRYVKDLCRLGRPLHKVILVDDSPHSYEFQPGNGIHIPPYTGALDDDSLLKVMKYLKGLPRDDLFRNTHQTPFRGGLMTELKHHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW72</accession>
    <name>I6ZW72_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265508.1">
        <property type="nucleotide sequence ID" value="XM_009267233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW72"/>
    <dbReference type="GeneID" id="20564628"/>
    <dbReference type="KEGG" id="ero:EROM_110290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110290"/>
    <dbReference type="HOGENOM" id="CLU_1896190_0_0_1"/>
    <dbReference type="OrthoDB" id="2191725at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15777" checksum="FCF103930836C271" modified="2012-10-03" version="1">MQAPFDKHISPTVPYVLQRGRRASPKIEGISKYIRHLRIPVRKNELFVAGGTTRQALEEAFHEVDLIEDIRVFKRYAFLSFKSNANIDHIPGKCHNIGGKPMYVDYVRRDDIKFIPIRLRNRASLWYNHKNKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW94</accession>
    <name>I6ZW94_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translation initiation factor beta propellor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_110540</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265533.1">
        <property type="nucleotide sequence ID" value="XM_009267258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW94"/>
    <dbReference type="GeneID" id="20564653"/>
    <dbReference type="KEGG" id="ero:EROM_110540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110540"/>
    <dbReference type="HOGENOM" id="CLU_035147_0_0_1"/>
    <dbReference type="OrthoDB" id="2194683at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="6">
        <location>
            <begin position="183"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="398"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="466" mass="53138" checksum="2B5FA2419D78995F" modified="2012-10-03" version="1">MPVVAHAEKGLTFNVFAGNTSWVECDYYALRSGRVAWIKGNLVRYYDLVDKKMILEIEMEKPREIEMSEDGRSVGILSGKGEMVVLGEDGIKLKAENVSRFRISNSLIGYVSSSSFYIHQIEEDKIEKSPFHASKVSVLEFFISGEFVFLATRKFEKDGMHKLLRIGRSSVDVLHSLKELQGFSLKTHKSGRYALLLLMTSYVNNSYFPESDVYFHDINRRSFKSLGYSPVHSYAFLSSGFAICHGSQPSSVSVHGLDGKLEYNFPKGIRNRIFFNQHENIAVFAGFDNLSGDIEVFHVPSRKLTAKLNVLGASLVDWKENGSYFYVSTTSYFQEDNKITLYDYYGRKIAERKFESLFSASVYGKTEEFIELERPERLNIETQKKYVPPSIHSFIPKTTFKSSTPKKKGLAGQKPNERTKDELLNDLKEIESLKNRMKSGEELSVKELNLILKESKLKSELKSFGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWE1</accession>
    <name>I6ZWE1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear scaffolding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111040</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC3/CDC27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265583.1">
        <property type="nucleotide sequence ID" value="XM_009267308.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWE1"/>
    <dbReference type="GeneID" id="20564703"/>
    <dbReference type="KEGG" id="ero:EROM_111040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111040"/>
    <dbReference type="HOGENOM" id="CLU_034743_0_0_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="343"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="475" mass="55780" checksum="C884845AEF21B47F" modified="2012-10-03" version="1">MDTKLIGRICKSIRYRDYETAIFLTACLTQQKSEYKTLMSIILYLNGEYSRALFHLHKLNTWTSKYYESLCYKKKKDYKKAIKSLELILDGKVERDPEVDARIQEMFIDPEDDEFFESLLGDLYTLSGYREEGIEHYIKSLAKSFLLPAVENLLLENKIPVKRDKENVRQMGKRGIEEEYVTDIIEFHESLGQSLIKKYLEYIPGVGSYFISNAARRYFNLGMNDKSKSCFELVRRKDPMFLHNVDYYSTILWHCKDVYELGMLCKNLIKHAPDSPNTWKALGNFYSHQGDYQRSVLCFKRSLCIEEDSYTYTLLGYESIQRNEYDVAMKYFNLSLKMLGDNYRAMFGCGLVYTKTERLDNAEFFLKKAIETNPGNLQIKVHAMKFYTRKGLTDQSIRLFKEAFCMKYTDIHKIVECILSRKGSFTNVEEFLVLEFVEILVLQNLSKLAADVLSCVEYRGESYSKKKELLQDRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWM3</accession>
    <name>I6ZWM3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_111900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265668.1">
        <property type="nucleotide sequence ID" value="XM_009267393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWM3"/>
    <dbReference type="GeneID" id="20564789"/>
    <dbReference type="KEGG" id="ero:EROM_111900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111900"/>
    <dbReference type="HOGENOM" id="CLU_1927570_0_0_1"/>
    <dbReference type="OrthoDB" id="2190929at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="132" mass="15241" checksum="B2B9973A5A3926F4" modified="2012-10-03" version="1">MREEYLQGLRRNIASELIEIKKAILEKMRHKLKETFSSVSMEAKNLPLLSLIEGLERNYTGKTIMIRKNKKIITKRVAKSTKKRPSVVIQVEDQSFVFDGDGLKVVGPWDGQQYKGMLDEINGILKKHITKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACW6</accession>
    <name>I7ACW6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="16">5.6.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">DNA repair helicase RAD25</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">RNA polymerase II transcription factor B subunit SSL2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Suppressor of stem-loop mutation 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ordered locus">EROM_010860</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25 26">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">ATP-dependent 3'-5' DNA helicase/translocase; binds dsDNA rather than ssDNA, unzipping it in a translocase rather than classical helicase activity. Component of the general transcription and DNA repair factor IIH (TFIIH) core complex. When complexed to CDK-activating kinase (CAK), involved in RNA transcription by RNA polymerase II. Also involved in transcription-coupled nucleotide excision repair (NER) of damaged DNA. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/SSL2, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/SSL2 is required for promoter opening and promoter escape.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. RAD25/XPB subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="16"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263927.1">
        <property type="nucleotide sequence ID" value="XM_009265652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACW6"/>
    <dbReference type="GeneID" id="20520713"/>
    <dbReference type="KEGG" id="ero:EROM_010860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010860"/>
    <dbReference type="HOGENOM" id="CLU_008213_0_0_1"/>
    <dbReference type="OrthoDB" id="10262986at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000112">
        <property type="term" value="C:nucleotide-excision repair factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18029">
        <property type="entry name" value="DEXHc_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18789">
        <property type="entry name" value="SF2_C_XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000077">
        <property type="entry name" value="Probable DNA repair helicase RAD25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000117">
        <property type="entry name" value="Putative DNA repair helicase rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050615">
        <property type="entry name" value="ATP-dep_DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032438">
        <property type="entry name" value="ERCC3_RAD25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001161">
        <property type="entry name" value="XPB/Ssl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032830">
        <property type="entry name" value="XPB/Ssl2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00603">
        <property type="entry name" value="rad25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION AND DNA REPAIR FACTOR IIH HELICASE SUBUNIT XPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11274">
        <property type="entry name" value="RAD25/XP-B DNA REPAIR HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16203">
        <property type="entry name" value="ERCC3_RAD25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13625">
        <property type="entry name" value="Helicase_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00851">
        <property type="entry name" value="XRODRMPGMNTB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 25" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="23">
        <location>
            <begin position="220"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="24">
        <location>
            <begin position="436"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="649"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="653"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00058901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="AFN82430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="672" mass="76864" checksum="B59C568EBBE771D6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACX7</accession>
    <name>I7ACX7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_011010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263942.1">
        <property type="nucleotide sequence ID" value="XM_009265667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACX7"/>
    <dbReference type="GeneID" id="20520728"/>
    <dbReference type="KEGG" id="ero:EROM_011010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011010"/>
    <dbReference type="HOGENOM" id="CLU_703156_0_0_1"/>
    <dbReference type="OrthoDB" id="2191528at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="206"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="374" mass="43522" checksum="267A51B91BA8DB98" modified="2012-10-03" version="1">MIDWLVENSLGLFPVVLERQLKKMEVGKKSPVLATIECSVSYILSIVPRNRKPEPGVLLVRGYQWSNGIRSRINFSVDLDAGSYYTIKDIQKTRMTNFSCHDRTVLYMFIINMDGKASMYYAQFSGCHSLRDPKVPLYRVELFHEDGFYRVDKDPSHDMILKSVNIRSKRKGLLHYCWSNQGILERQPRASFVLETEEKKYNVHGFCCIFCIREFECIDSLILHINYIHSGYKCIQNGRSLFLKRNFSSLQSHEALAYFSRKYKRKGLESRIAQDISQRHESPVNGIWSIENLSALINKGIRSNSGLSEDTLILMERWNVLRLHGGMLIDDIARFARQEKRNPSIVNFLLVLYHKSIVNPKELTELIYSLFEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACY5</accession>
    <name>I7ACY5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclear movement protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_011110</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263952.1">
        <property type="nucleotide sequence ID" value="XM_009265677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACY5"/>
    <dbReference type="GeneID" id="20520738"/>
    <dbReference type="KEGG" id="ero:EROM_011110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011110"/>
    <dbReference type="HOGENOM" id="CLU_124011_0_0_1"/>
    <dbReference type="OrthoDB" id="416217at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06467">
        <property type="entry name" value="p23_NUDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037898">
        <property type="entry name" value="NudC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356:SF3">
        <property type="entry name" value="NUCLEAR MIGRATION PROTEIN NUDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12356">
        <property type="entry name" value="NUCLEAR MOVEMENT PROTEIN NUDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="2"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="135" mass="15468" checksum="F01531F96D4941C9" modified="2012-10-03" version="1">MPLEAKYTWDQELNEINIRFPMAEGADSNSVKISVVGKKVLMKMQEEVIMDGEFLHEVDSSSLWWVIDGDSVDINITKKRNEWWDSLLVGSESVDVQKLAEDKHADISMLDPEAREVVEKMMHNTNKKDPSDLCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADA0</accession>
    <name>I7ADA0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 5'-3' helicase</fullName>
            <ecNumber evidence="9">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_021000</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="9"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264072.1">
        <property type="nucleotide sequence ID" value="XM_009265797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADA0"/>
    <dbReference type="GeneID" id="20520862"/>
    <dbReference type="KEGG" id="ero:EROM_021000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021000"/>
    <dbReference type="HOGENOM" id="CLU_006515_6_1_1"/>
    <dbReference type="OrthoDB" id="272481at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990918">
        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18788">
        <property type="entry name" value="SF2_C_XPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010614">
        <property type="entry name" value="RAD3-like_helicase_DEAD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF47">
        <property type="entry name" value="FANCONI ANEMIA GROUP J PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06733">
        <property type="entry name" value="DEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="7"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="678" mass="76239" checksum="278290A9894D95B8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADB1</accession>
    <name>I7ADB1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_021100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264082.1">
        <property type="nucleotide sequence ID" value="XM_009265807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADB1"/>
    <dbReference type="GeneID" id="20520872"/>
    <dbReference type="KEGG" id="ero:EROM_021100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021100"/>
    <dbReference type="HOGENOM" id="CLU_1517853_0_0_1"/>
    <dbReference type="OrthoDB" id="2191981at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20833" checksum="E273BF66A7B3E8E5" modified="2012-10-03" version="1">MNDITFSKRIEILDECIRKLEVDASKERESKLEDMFRICDRLVECGQQSPKLVGQYNELKNRYRCIARPYKELDDEISACKMHMEVLSRKDTINEVARSVQEIIAVSDYINYAINDAIFPIDNVMEHLEEGEQYGILSNEQLSISRRRKVWKVRIIRSMLLIVFTLAAIFMVVRFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADU3</accession>
    <name>I7ADU3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264257.1">
        <property type="nucleotide sequence ID" value="XM_009265982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADU3"/>
    <dbReference type="GeneID" id="20521052"/>
    <dbReference type="KEGG" id="ero:EROM_031380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031380"/>
    <dbReference type="HOGENOM" id="CLU_169138_0_0_1"/>
    <dbReference type="OrthoDB" id="442087at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="1">
        <location>
            <begin position="2"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="13475" checksum="E1C351EA1448C880" modified="2012-10-03" version="1">MPIFSLLRLRKGATKEEVRIRYLRRLIQIHPDRNKGNGAEYTKLRNAYEKYIRGEVFGGEPYLVCSRTDVESAICRCGGKYKISHEVDGRIECEFCSCFIEIEELLELSDTSRKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADV8</accession>
    <name>I7ADV8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_040090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264277.1">
        <property type="nucleotide sequence ID" value="XM_009266002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADV8"/>
    <dbReference type="GeneID" id="20521073"/>
    <dbReference type="KEGG" id="ero:EROM_040090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040090"/>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_1"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="4">
        <location>
            <begin position="100"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="241" mass="27044" checksum="77BB2BF0813958B8" modified="2012-10-03" version="1">MSRYFVNSGVIRKQKYGKLLSKCTSYWVGSFCCKLEVKGIAYKNHKKIYPQRMSEIKLFEKYSYEDVKVDNISLKPYINLSRHGIVPHAATTITKGTTGKARIPIAERFVCSLMRHGRNNGKKRLAINIFEDACFIIHSMTKKNPLQVLVDAIINSGPREDTARIGRAGSMRRTSVDVSPLKRISIAIANLSTGIRSASFRNRITLAEAIANELMAASTGSQNSYAVNKKKEIERIAQSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADZ5</accession>
    <name>I7ADZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuolar protein sorting-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264327.1">
        <property type="nucleotide sequence ID" value="XM_009266052.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521126"/>
    <dbReference type="KEGG" id="ero:EROM_040620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040620"/>
    <dbReference type="HOGENOM" id="CLU_229509_0_0_1"/>
    <dbReference type="OrthoDB" id="2191600at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF93">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="2386" mass="269604" checksum="DE278CA0218680A3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE27</accession>
    <name>I7AE27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Translation elongation factor EF-1 alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_041080</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264372.1">
        <property type="nucleotide sequence ID" value="XM_009266097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE27"/>
    <dbReference type="GeneID" id="20521172"/>
    <dbReference type="KEGG" id="ero:EROM_041080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041080"/>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_1"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01513">
        <property type="entry name" value="Translation_factor_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="4">
        <location>
            <begin position="10"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="470" mass="51747" checksum="2DB6DBC534E5D468" modified="2012-10-03" version="1">MATKVEDDNKPRLNVCFIGHVDSGKSTTVGMLSHQMGVIDKRELEKYEKEAAMNNKDTFYLAYLTDKTEAERKRGITITTTLVTLPTEKFNVNILDCPGHKDFVKNMVTGASQADVAVVIVPASGFESCIGVGGMLKTHIMISGILGCEKLIICVNKMDMISEDKRKEKFKEVSDEMLRIVTRSHKDKNPIILPISGLMGLNLTKKGDSFKWFEGWREKEGAQAIYTLEEALDYQDVPKRHNDKPLRMPITKVCSISGVGKIFTGRVEYGTITPNLKISIQPAGVVGEIRSVEIHNKPRSMIPCGENCGVALKGGVTGDLSKVDAGHVISANDENKAIAYPGAKVKIIVVEKPNGMEVGYTPQINFGICRSPARVAKILSKVVKKKGHENPEKIINGEAFIGIVVFQKPLVIDKMERFQNLSKFALMDSNGVVAIGNVMEPLTREQLLEDYEIDIAEDPKAAKKASKKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE41</accession>
    <name>I7AE41_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA-binding domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_041290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264392.1">
        <property type="nucleotide sequence ID" value="XM_009266117.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE41"/>
    <dbReference type="GeneID" id="20521192"/>
    <dbReference type="KEGG" id="ero:EROM_041290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041290"/>
    <dbReference type="HOGENOM" id="CLU_924453_0_0_1"/>
    <dbReference type="OrthoDB" id="2196320at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033">
        <property type="entry name" value="RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48033:SF10">
        <property type="entry name" value="RNA-BINDING PROTEIN SQUID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="141"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="218"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="304" mass="35636" checksum="6DD0CFB351643824" modified="2012-10-03" version="1">MRDRMKKKGGGKHKLKCKEKSKRHCRDISNISNISHTEERKKNARGRSMCKKISKEDDETDKKRRRYQKIEFDEATCSVTVKGNSTLGDIKKTFKNFKDMKSGEDGFVLEYPSKEHALVDIKANRRQGGEKKEDSSRYLSNKYILTNLSYKEDKESISKVFKKYGEIERITIKKNKDNISTGKAIITFKKRAVIKEEIVMSNKPIYIERIKKPLENKTRFFLGKLTKSLSIVEIRKIFAKVECKPKDIRILYGENKRNRGYGFIEYANEKDANVFIERFEKIKSLLGPECYYEYSNEKVSSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE62</accession>
    <name>I7AE62_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264417.1">
        <property type="nucleotide sequence ID" value="XM_009266142.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE62"/>
    <dbReference type="GeneID" id="20521219"/>
    <dbReference type="KEGG" id="ero:EROM_041560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041560"/>
    <dbReference type="HOGENOM" id="CLU_018687_5_0_1"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="27"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="330" mass="38840" checksum="75323241B7940EE5" modified="2012-10-03" version="1">MENSPDNKVFGENIIDEKLVRKLCFGGVRSEYRCIAWRIIFQVIGLRKQLHMNEIEARQRKYISMATKMGCSWVKDADRYSMKDPMGKNGLMKLNGIYHYGKIGLPEKIAHQIDLDIKRIDPRYKTYSGIDISYMYYHILWLIARRRPQLGYVQGMADILVPFVLVFSDESVETAESNAYFCYARLLDEIQHNIIDLQAGMIKGVDLVLQTVDPDFHKFLKDIGLEIHMFAFRWFNCFFAREFKIPILLKVLDTVFASDNINESLLYFGVALLMRLKPVLIENTFSHNILLLQSIYQREWEEAEIELILSSAKFYRKVTSRQLIWSRFWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEC7</accession>
    <name>I7AEC7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264507.1">
        <property type="nucleotide sequence ID" value="XM_009266232.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEC7"/>
    <dbReference type="GeneID" id="20521310"/>
    <dbReference type="KEGG" id="ero:EROM_050780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050780"/>
    <dbReference type="HOGENOM" id="CLU_2454733_0_0_1"/>
    <dbReference type="OrthoDB" id="10374174at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="96" mass="11471" checksum="D311513E7A6D09E7" modified="2012-10-03" version="1">MKKSPKRKEVEDKCEVIEEEREEERENNSFDENLRREMCEKVLRFIDKNAIDEIRELIRYGLYEDLQEFVNPWIASEVHGILNDVSAIQAAIDDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEJ1</accession>
    <name>I7AEJ1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Replication factor C subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_051600</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264587.1">
        <property type="nucleotide sequence ID" value="XM_009266312.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEJ1"/>
    <dbReference type="GeneID" id="20521392"/>
    <dbReference type="KEGG" id="ero:EROM_051600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051600"/>
    <dbReference type="HOGENOM" id="CLU_003574_8_0_1"/>
    <dbReference type="OrthoDB" id="446168at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17748">
        <property type="entry name" value="BRCT_DNA_ligase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000395">
        <property type="entry name" value="Replication factor C subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013725">
        <property type="entry name" value="DNA_replication_fac_RFC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012178">
        <property type="entry name" value="RFC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08519">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036578">
        <property type="entry name" value="RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="9">
        <location>
            <begin position="3"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="544"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="549"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="567" mass="63995" checksum="DAA0A32CB215073F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEP8</accession>
    <name>I7AEP8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosomal RNA-processing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_060300</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264622.1">
        <property type="nucleotide sequence ID" value="XM_009266347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEP8"/>
    <dbReference type="GeneID" id="20521427"/>
    <dbReference type="KEGG" id="ero:EROM_060300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060300"/>
    <dbReference type="HOGENOM" id="CLU_1240128_0_0_1"/>
    <dbReference type="OrthoDB" id="45882at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF8">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0271">Exosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="223" mass="25297" checksum="CE7AB2963C4D517C" modified="2012-10-03" version="1">MIAKNLAEVKERFDNRDIEEARGFEVERISNSCLVSMGTSRLLTVFQKTVSKPYIDRPHEGIISFSVSIGGKRHEKLLNFLHKVYTKQKSIDLESLCVKLNEEVTFIHIDLRILSSDGNIYSLAVKGVNSVLEVLGVKMNYMPQCFFYCSIGNVIVTDPSEDEQKEEDWSCIVVMKSFREIIFLEKVGKECGIEQVLEAVDRSTGNFAMRIPEIESEEECGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEQ8</accession>
    <name>I7AEQ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribonuclease Z</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264627.1">
        <property type="nucleotide sequence ID" value="XM_009266352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEQ8"/>
    <dbReference type="GeneID" id="20521433"/>
    <dbReference type="KEGG" id="ero:EROM_060360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060360"/>
    <dbReference type="HOGENOM" id="CLU_2386142_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707780" evidence="1">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="94" mass="10321" checksum="CA20394F2D92FBBF" modified="2012-10-03" version="1">MSLSFSFLLTSSASLFHCLSVLTGIPNILRARASISLSPISDLLFFIFKSFHINSSSFIAIFSRPVNATSMDRSISVLAMDSWPTNVATVIYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AES6</accession>
    <name>I7AES6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264642.1">
        <property type="nucleotide sequence ID" value="XM_009266367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AES6"/>
    <dbReference type="GeneID" id="20521448"/>
    <dbReference type="KEGG" id="ero:EROM_060510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060510"/>
    <dbReference type="HOGENOM" id="CLU_081169_0_0_1"/>
    <dbReference type="OrthoDB" id="2190145at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="266" mass="30516" checksum="EF5267425A41167F" modified="2012-10-03" version="1">MKPIPFVAIIPKIMSWYEPAIDVNKVPRDDMILRPHESESTNDREYTPGSSAKGAPESSPDQEISLSDIDEKINEYPSKEITPPQGKSIPIADDVKAKIDKVDELTKAFNERIGTTKVSGIHDKEAWNKLYEYLRLNFNDFLIDGHLSIELHIRYILCCYVVAFIPGQFAFLFPAFYKVFIPKRAMKVPILGDALKGIDRFLTFVSDRSTKTFIITFYVGRQNSLVEDIEFVKKVPDLIFTDPMAILREILKFEIRSEGYDNKKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AET3</accession>
    <name>I7AET3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264652.1">
        <property type="nucleotide sequence ID" value="XM_009266377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AET3"/>
    <dbReference type="GeneID" id="20521458"/>
    <dbReference type="KEGG" id="ero:EROM_060610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060610"/>
    <dbReference type="HOGENOM" id="CLU_1034492_0_0_1"/>
    <dbReference type="OrthoDB" id="73919at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="CDD" id="cd00821">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="2">
        <location>
            <begin position="167"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="272" mass="31631" checksum="D1628D1676F78288" modified="2012-10-03" version="1">MEDKDMRKKKKNIIIINDKGEMLSVTKKTLDGNKDGEDMASHEEVKEMNGEEDTRIDTEEKIESTGEAFETKADERSHEKECSESKECCGGPKNEEGQGRKETMAEENSKNDPEEEFIPLDSKEPGEKAESVKSPPKESGDVFVPPMLKTTTENANKEIFEERDKGEIVAEGWIWKKRRIFSCFWHQKYFVLTKDGTLKYYKADGRRHAKGNWNMKESTEIRHYNLSSEENTHPCRIMVFFSDHSFLLAFDERNTKDYWVEKLNRAIGKPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEV9</accession>
    <name>I7AEV9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA 3'terminal phosphate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264687.1">
        <property type="nucleotide sequence ID" value="XM_009266412.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEV9"/>
    <dbReference type="GeneID" id="20521494"/>
    <dbReference type="KEGG" id="ero:EROM_060980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060980"/>
    <dbReference type="HOGENOM" id="CLU_027882_1_0_1"/>
    <dbReference type="OrthoDB" id="2194813at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF1">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="2">
        <location>
            <begin position="170"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="341" mass="37714" checksum="839AC9EC157127EC" modified="2012-10-03" version="1">MNCVNEMIFDCEKSLELILVYSLLSKSKVKVLVKRRQKMAKSFAKLLQKISHGTAYSLSEGYMAFTPGSLKGGEVAIQCEEEISLYIEPILVLCPFVIEPLKIKFKGITNGKLCTDLIRIAHFDVLKRFGVKECEVVVKKRGFGPVGKGEVVFTVDSPGKISTVRLERLEKIVKIRGLVLSSRVSSIPTKEMTERIKELMGDVSNTKVFSNMSNRLDSGPSPGFQCAVFAESKNGIYYSVMSGEGLTPKETATIACKDLLRSIRHGGVFDKKLLYLALSLMSLSSPSIGSLWVGKVDSDVKIILDLLRQFFGFEYEIRKSGEGDVVYGAGCGLSRIPRQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEY3</accession>
    <name>I7AEY3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RING-CH-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_061300</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264717.1">
        <property type="nucleotide sequence ID" value="XM_009266442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEY3"/>
    <dbReference type="GeneID" id="20521526"/>
    <dbReference type="KEGG" id="ero:EROM_061300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061300"/>
    <dbReference type="HOGENOM" id="CLU_096550_0_0_1"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16495">
        <property type="entry name" value="RING_CH-C4HC3_MARCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF3">
        <property type="entry name" value="FI20425P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="11">
        <location>
            <begin position="28"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="250" mass="29262" checksum="671DFE33E883A194" modified="2012-10-03" version="1">MEGVECGADFQAGKKQDGTSLSVTVPRKITKDSRFCKICYSLTNPINMKDDLISPCNCKGSIGLVHGVCLKMWRYRGKRIKDIRKCEQCSSFYKLDNEIVPHRIVVSLATVIILLLIYLVSTILFKSLIDAFIIIIRDFFFADVHTLVDLEMGRGLEYVFLQKQSPLEYKFIETNGFVYLFIMVLLYQVFSKISFFSIFNYMFTFWRLNQFDFALDKVLFGFMSMYYMRKAYNDIYSRVDSGLIFFLNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF92</accession>
    <name>I7AF92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Splicing factor 3a subunit 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264857.1">
        <property type="nucleotide sequence ID" value="XM_009266582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF92"/>
    <dbReference type="GeneID" id="20521669"/>
    <dbReference type="KEGG" id="ero:EROM_071090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071090"/>
    <dbReference type="HOGENOM" id="CLU_727800_0_0_1"/>
    <dbReference type="OrthoDB" id="2189371at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024598">
        <property type="entry name" value="SF3a60/Prp9_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11931">
        <property type="entry name" value="SF3a60_Prp9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Splicing factor SF3a60 /Prp9 subunit C-terminal" evidence="1">
        <location>
            <begin position="274"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="375" mass="44546" checksum="7EFB0ACCF476B5B9" modified="2012-10-03" version="1">MTNFDVIKDMDERIVKRQIGILENMERISNWMVEIGKPTSKKDKMVKRYTRSILKRKYKRYRRLGVLNIEEKLEGKIKRLKDFGHYESRALKEVEIVFKDSRLYGAGLELDKIFKKYTDVHLCDDYKKFLKSICKLNVDKSRFRYLEYLNELKEYLSGVIKIKYFGMFRKFMASTPRIIERAEACSFSEVNGVEGVFPKVYCVKCSREISRSVFGYHLKGKRHCKKEREEVLFCGMPVLKAEGLIKKALEILDRERRYSISRLSRKKKRLRSDVPKWLYKREELDISFECDICGYSGYGRDGFDRHFGEDSHRKSVARYGIKYSPALKGITRVGILMKMKDRVEESESYTEEFEDGDGNVFDRRTYEDLKRNNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFD4</accession>
    <name>I7AFD4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_071640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264912.1">
        <property type="nucleotide sequence ID" value="XM_009266637.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFD4"/>
    <dbReference type="GeneID" id="20521728"/>
    <dbReference type="KEGG" id="ero:EROM_071640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071640"/>
    <dbReference type="HOGENOM" id="CLU_1619551_0_0_1"/>
    <dbReference type="OrthoDB" id="2189300at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052084">
        <property type="entry name" value="SF3B4_spliceosome_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48030:SF3">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="10"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="153" mass="17931" checksum="A6CD114A085C2F8C" modified="2012-10-03" version="1">MERGLTDEKHKLFITNIPTKTRKQLVYELLTQVSKISNLYYNQDKGYCFVSYETHEELDYTYKVLQGVKLYGKRLYFSKVEEQVRVVVRNIGQEIDEVFLWDVFSKFGPCHVEIDGDGLGIVVYRRKDSAIKAVDTVNRKIIGYSEVFVEIDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFJ6</accession>
    <name>I7AFJ6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">poly(A)-specific ribonuclease</fullName>
            <ecNumber evidence="5">3.1.13.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_080820</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
            <dbReference type="EC" id="3.1.13.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CAF1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.4" evidence="5"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264997.1">
        <property type="nucleotide sequence ID" value="XM_009266722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFJ6"/>
    <dbReference type="GeneID" id="20521817"/>
    <dbReference type="KEGG" id="ero:EROM_080820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080820"/>
    <dbReference type="HOGENOM" id="CLU_027974_0_1_1"/>
    <dbReference type="OrthoDB" id="1164111at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000048">
        <property type="entry name" value="CCR4-associated factor 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039637">
        <property type="entry name" value="CNOT7/CNOT8/Pop2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006941">
        <property type="entry name" value="RNase_CAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10797">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04857">
        <property type="entry name" value="CAF1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="261" mass="30009" checksum="4617F126DE3AD549" modified="2012-10-03" version="1">MSECQILNVWKDNLRSEMRRISQLVNTYNYISMDTEFPGVVAKPIGSFKSPSSFAYQQLRCNVDILNIIQLGISLSDSQGNRPCPISTWQFNFAFSLETDMYAQESIDLLIQARIDFKEHERRGIKVEEFGEVLMTSGLVMSPDVIWVSFHSAYDFGYLIKILTCNPLPEREEDFYRFLAALFPDFYDIKFLVQNSKYLKKGLQEISNDLGLVRDGIQHQAGSDALLTSHAFFKTREVLFNKNIGKELMCKLYGIEVKESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG27</accession>
    <name>I7AG27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S15A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090730</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265187.1">
        <property type="nucleotide sequence ID" value="XM_009266912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG27"/>
    <dbReference type="GeneID" id="20564297"/>
    <dbReference type="KEGG" id="ero:EROM_090730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090730"/>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_1"/>
    <dbReference type="OrthoDB" id="10250260at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="128" mass="14584" checksum="11343E1626D29AE0" modified="2012-10-03" version="1">MSAALANMCKAINNASRAGKRQLLLRFSTEEMRKFLFQMLKYGYISGFSHIHDKRNGKSIIDLNGRLNRCGAISPNYIVKRNGIENFRARLLPARQFGHVLFNTSKGILDHKECLEENVGGKILGYFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGB8</accession>
    <name>I7AGB8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Undecaprenyl pyrophosphatase synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265302.1">
        <property type="nucleotide sequence ID" value="XM_009267027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGB8"/>
    <dbReference type="GeneID" id="20564415"/>
    <dbReference type="KEGG" id="ero:EROM_091910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091910"/>
    <dbReference type="HOGENOM" id="CLU_086428_0_0_1"/>
    <dbReference type="OrthoDB" id="4173905at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="271" mass="32139" checksum="F42B7152909957CE" modified="2012-10-03" version="1">MLKGLKIRSLVCIVEIIEGIINNIHIIKIALFMMRILKGAYILIWKILCMWNGRPYNYSTESNRRSRLTIFKNMNVAFICDGNRRYMKKLGLEDRFTKDEGLQKVYEFINFGYFYGFEEISFFCFALNNLKRTPDEVNGMMEVVKKKVKEPKEVGIRPRFRIYGRLDLLEEDVRNRLMDIERESRNNTDIIVNIFFAYSSEDEISRGIQFNSRVDILIRTGDTKRLSNFMIRQVAKGTSVFFAKPLWPELTTAHLFLILLKHRLEDRYLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGK9</accession>
    <name>I7AGK9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoglycerate mutase (2,3-diphosphoglycerate-independent)</fullName>
            <ecNumber evidence="4">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_100940</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BPG-independent phosphoglycerate mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="4"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265407.1">
        <property type="nucleotide sequence ID" value="XM_009267132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGK9"/>
    <dbReference type="GeneID" id="20564524"/>
    <dbReference type="KEGG" id="ero:EROM_100940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100940"/>
    <dbReference type="HOGENOM" id="CLU_026099_2_0_1"/>
    <dbReference type="OrthoDB" id="1886626at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006007">
        <property type="term" value="P:glucose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16010">
        <property type="entry name" value="iPGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1450.10">
        <property type="entry name" value="BPG-independent phosphoglycerate mutase, domain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01038">
        <property type="entry name" value="GpmI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011258">
        <property type="entry name" value="BPG-indep_PGM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036646">
        <property type="entry name" value="PGAM_B_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005995">
        <property type="entry name" value="Pgm_bpd_ind"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01307">
        <property type="entry name" value="pgm_bpd_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637">
        <property type="entry name" value="2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31637:SF0">
        <property type="entry name" value="2,3-BISPHOSPHOGLYCERATE-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06415">
        <property type="entry name" value="iPGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001492">
        <property type="entry name" value="IPGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64158">
        <property type="entry name" value="2,3-Bisphosphoglycerate-independent phosphoglycerate mutase, substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="12">
        <location>
            <begin position="3"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="BPG-independent PGAM N-terminal" evidence="13">
        <location>
            <begin position="82"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="9">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="146"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="248"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001492-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="504" mass="55954" checksum="B0190ED324991B56" modified="2012-10-03" version="1">MSNVCLVVIDGWGYDEASTRGNAVNESRCQWMKTLSQSRHSLLLHAHGRYVGLPDGLMGNSEVGHLTIGSGRVIEQDILRIDKAIEEGRLRSMLRKEVQEVEGKIHLVGMVSDGGVHSHIRHLKGILESLRDLGEEVFIHCVSDGRDTEPRVFLKYLSEVEGFCQKIGIGKVASIAGRFYTMDRASNNKRTELSFQEMTSGEEVQGEVRDYILGMYEEGLSDETLRPFLVDKRGKIEPEDTIIFFNFRADRMRQITSRFAENGNNIITMTEYKKELKSKVLFEKVCVKNTLAEIMSSHGMIHSHIAEKEKEAHVTYFFNGGKEQAFLNQKTIIHPSPNVESFDAAPSMASKEVTSSVIAEIGSGVPLVIANLAPPDMVGHTGNLEATKMAIEFTDECIGSIYKACKSNGYTLIVTADHGNAEKMIDEEGNCCKTHTTSKVPLIICEGKPGRPSSSWGYIDSNYSLRDVAPTVLEIMGIPKPKEMTGRSVLKSARYPLSSDKEPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH56</accession>
    <name>I7AH56_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Apurinic-apyrimidinic endonuclease 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_111440</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AP endonuclease 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265622.1">
        <property type="nucleotide sequence ID" value="XM_009267347.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH56"/>
    <dbReference type="GeneID" id="20564743"/>
    <dbReference type="KEGG" id="ero:EROM_111440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111440"/>
    <dbReference type="HOGENOM" id="CLU_025885_0_1_1"/>
    <dbReference type="OrthoDB" id="7663182at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000001">
        <property type="entry name" value="Probable endonuclease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00152">
        <property type="entry name" value="Nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018246">
        <property type="entry name" value="AP_endonuc_F2_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00587">
        <property type="entry name" value="nfo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00729">
        <property type="entry name" value="AP_NUCLEASE_F2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00730">
        <property type="entry name" value="AP_NUCLEASE_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00731">
        <property type="entry name" value="AP_NUCLEASE_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51432">
        <property type="entry name" value="AP_NUCLEASE_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="9">
        <location>
            <begin position="22"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="275" mass="30898" checksum="470054CF5A633352" modified="2012-10-03" version="1">MAKLIGAHLSISKGIHTIQAQMESLGLDTCAIFLKSQRRYLAPPMREGVAEMFKARVLHPEMIVPHGSYLINFGNPSLVDKSMECLVDDLERCRSLGIKMYNMHPGSDKTRDKTGCLRSISHNINKALSRVQDITILIENMAGQGNVVGSTFEELRDIIEKVEERERIGICLDTCHLFGAGFDITTEERFAEVMERFDRIIGLKFLKAMHINDSKEALGSRKDRHESIGKGMIGGKAFRFIMNSGVFDNIPLILETPDPGKYKEEAGFLRNLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL25</accession>
    <name>I7AL25_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263846.1">
        <property type="nucleotide sequence ID" value="XM_009265571.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL25"/>
    <dbReference type="GeneID" id="20520630"/>
    <dbReference type="KEGG" id="ero:EROM_010040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010040"/>
    <dbReference type="HOGENOM" id="CLU_132184_0_0_1"/>
    <dbReference type="OrthoDB" id="2189688at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008584">
        <property type="entry name" value="CXXC_Zn-binding_euk"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05907">
        <property type="entry name" value="CXXC_Zn-b_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141678">
        <property type="entry name" value="MAL13P1.257-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="19241" checksum="477DBBC0A4EE4894" modified="2012-10-03" version="1">MHFNVFVEGEFNNIKGVFIDQSYPLRIRCTNCGAPHEKVVVLSDDSVAEGDFKEKVNLSVNCRSCKRMMTLKILKLKEAKKHMLPTKFEDEFKEVWFTDMEKSRFLVSRIETNGAEVMSIESCILNMVSNQDVLFTNADFEDKIVAKCNNLNTISSITRFSLVVELAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL28</accession>
    <name>I7AL28_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_010090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263851.1">
        <property type="nucleotide sequence ID" value="XM_009265576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL28"/>
    <dbReference type="GeneID" id="20520635"/>
    <dbReference type="KEGG" id="ero:EROM_010090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010090"/>
    <dbReference type="HOGENOM" id="CLU_036235_0_2_1"/>
    <dbReference type="OrthoDB" id="10267824at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="4">
        <location>
            <begin position="82"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="25882" checksum="207549585C7A98C7" modified="2012-10-03" version="1">MGKITRRTREAKKKERQPIVKVSLKYPIVPQVEEGEVIDIVHERSRGAPVAIISFKELDCMVPASEGIYTGKKVLIGDDAPIDIGNITKIKNVPEGMAVNSVEVVYGDGGCISMTNGSYSLVVNHRKETNETVIKIPSGKKITVNSECRCIVGVVAGGGIHDKPLLKASVAHYRAKARGHIFPRVRGVAMNPVEHIHGGGNHQHVGKPTTVSNKDISQEQKIGLVGARRTGYRVGSRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL47</accession>
    <name>I7AL47_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">FeS cluster assembly scaffold Isc</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_010290</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NifU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263871.1">
        <property type="nucleotide sequence ID" value="XM_009265596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL47"/>
    <dbReference type="GeneID" id="20520656"/>
    <dbReference type="KEGG" id="ero:EROM_010290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010290"/>
    <dbReference type="HOGENOM" id="CLU_079283_5_0_1"/>
    <dbReference type="OrthoDB" id="1925777at2759"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06664">
        <property type="entry name" value="IscU_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1010.10:FF:000008">
        <property type="entry name" value="Iron-sulfur cluster assembly enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011339">
        <property type="entry name" value="ISCU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002871">
        <property type="entry name" value="NIF_FeS_clus_asmbl_NifU_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01999">
        <property type="entry name" value="iscU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10093">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01592">
        <property type="entry name" value="NifU_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82649">
        <property type="entry name" value="SufE/NifU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIF system FeS cluster assembly NifU N-terminal" evidence="8">
        <location>
            <begin position="14"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="140" mass="15199" checksum="261B6078C22527E9" modified="2012-10-03" version="1">MPALQDISRITRKYDASVVDHFENPRNVGSLDKADPRVGTGIVGAPACGDVMKLQIKVGKDNVIEDAKFKTFGCGSAIASSSLATEWIKKKTLDESLKISNRDIAKKLSLPPIKLHCSMLAEDAIKSAIKDFLDKNRPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL87</accession>
    <name>I7AL87_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010750</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Transcriptional coactivator that stimulates GCN4-dependent transcriptional activity by bridging the DNA-binding region of GCN4 and TBP (SPT15), thereby recruiting TBP to GCN4-bound promoters. Involved in induction of the ribosome quality control (RQC) pathway; a pathway that degrades nascent peptide chains during problematic translation. Required to prevent stalled ribosomes from frameshifting.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MBF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263916.1">
        <property type="nucleotide sequence ID" value="XM_009265641.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL87"/>
    <dbReference type="GeneID" id="20520702"/>
    <dbReference type="KEGG" id="ero:EROM_010750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010750"/>
    <dbReference type="HOGENOM" id="CLU_176427_0_0_1"/>
    <dbReference type="OrthoDB" id="2188935at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245:SF15">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10245">
        <property type="entry name" value="ENDOTHELIAL DIFFERENTIATION-RELATED FACTOR 1 MULTIPROTEIN BRIDGING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="41"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00035107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="95" mass="10889" checksum="F279E7AB695298BD" modified="2012-10-03" version="1">MRDNKPIILTKKKPEGQNRPKGPNPEDVEVKMVSKEVGDAIANARTRKGMSRKDLAQKLKKNVSVIDSWERGEAPYNEKIASEIEKLLEFKINWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL93</accession>
    <name>I7AL93_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CDP-diacylglycerol</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_010800</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263921.1">
        <property type="nucleotide sequence ID" value="XM_009265646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL93"/>
    <dbReference type="GeneID" id="20520707"/>
    <dbReference type="KEGG" id="ero:EROM_010800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010800"/>
    <dbReference type="HOGENOM" id="CLU_067602_2_0_1"/>
    <dbReference type="OrthoDB" id="10251079at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003881">
        <property type="term" value="F:CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006661">
        <property type="term" value="P:phosphatidylinositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF4">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--INOSITOL 3-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="131"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="189" mass="21484" checksum="2535943DAD43A28A" modified="2012-10-03" version="1">MKLSVMFNVPNTIGYFRIGLLIASTFVSGSAFVVLYGLSSLLDFFDGHLARMLNQCSLLGSSLDMITDRVSTVVISLRIIQKRVEYLQILSLYIIFDLISHFIYFHMSTLLGKHHKGTSNRILRVYYDKRVLGPTCLLSEVFFMYIYCFDYKGVLLYTLGGVTMVKTLFHLAQLSEAVSGMSDIRCIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALB0</accession>
    <name>I7ALB0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Replication factor C large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_011000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263941.1">
        <property type="nucleotide sequence ID" value="XM_009265666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALB0"/>
    <dbReference type="GeneID" id="20520727"/>
    <dbReference type="KEGG" id="ero:EROM_011000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011000"/>
    <dbReference type="HOGENOM" id="CLU_717679_0_0_1"/>
    <dbReference type="OrthoDB" id="2195431at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="38"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="383" mass="44333" checksum="8FF0BA88BDBF0425" modified="2012-10-03" version="1">MRSLVWAEKYRPQKYTDLVFKGGVHHEALKWLRDYPNHGRILLINGPPGTGKTSLVHILSSVFGLNLIEFNASNGSGCVDKILNVYGTVNGKKNLILIDEIDSLPSVDVETLISSTKLIYPVVMISNEMHLEDVYTMEIKRPGIEEVRKCIERVCKEEMVNVDNSALSQLVEDSDGDFRAIINYLQIHKQGLTNSKDLRIGKSVPITRHRAVELILTKHVRWKTYEDVYSTDVLSLCHSSFPYNTSLMRLIADISESTSLADTLAEEFRYMTLSKYNGCNSRKADIHRVEHYREPIHDLAKERILPYFRKYDLNRTDRRSLSHLKEIMEMYSITDVKLKEEDRMEDSISKGSRKFKFRYKPGCSSAFRRDVTMDDVMDWFFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALB8</accession>
    <name>I7ALB8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="7">2.4.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Inosine-guanosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_011100</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Cleaves guanosine and inosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the PNP/MTAP phosphorylase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="7"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263951.1">
        <property type="nucleotide sequence ID" value="XM_009265676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALB8"/>
    <dbReference type="GeneID" id="20520737"/>
    <dbReference type="KEGG" id="ero:EROM_011100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011100"/>
    <dbReference type="HOGENOM" id="CLU_054456_1_0_1"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09009">
        <property type="entry name" value="PNP-EcPNPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000004">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011270">
        <property type="entry name" value="Pur_Nuc_Pase_Ino/Guo-sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011268">
        <property type="entry name" value="Purine_phosphorylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01700">
        <property type="entry name" value="PNPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01697">
        <property type="entry name" value="PNPH-PUNA-XAPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006054">
        <property type="entry name" value="PRK08202.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904">
        <property type="entry name" value="METHYLTHIOADENOSINE/PURINE NUCLEOSIDE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11904:SF9">
        <property type="entry name" value="PURINE NUCLEOSIDE PHOSPHORYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000477">
        <property type="entry name" value="PurNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="8">
        <location>
            <begin position="43"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="307" mass="33682" checksum="639279908DEEA4FF" modified="2012-10-03" version="1">MISETKSSNCFSNDSHDTGPKYTYELVKDISDFVMKRIHIKPKVGIICGSGMGAIPEELTEKIEIEYKDIPHFPISTVQGHDGKLVTGKMGRVGVICMQGRFHYYEGYPLSTCSMPVRMMKLCGITHIIISNAAGGINPEYNIGDIMIIKDHINFLGYGGVNPLVGAHDERWGPRFVPVNKIYSEEVREKAKIAAQRLGIESFLREGVLSITGGPCYETVAESRMLHKLGADAVGMSVIGEAITACQAGLTVFGFSLIAGKCPMEYSVDSEISHSEVVECAQKREILIRKWVLELVGILIPSLPSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALR6</accession>
    <name>I7ALR6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Kinetochore protein Spc24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_021390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPC24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264111.1">
        <property type="nucleotide sequence ID" value="XM_009265836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALR6"/>
    <dbReference type="GeneID" id="20520901"/>
    <dbReference type="KEGG" id="ero:EROM_021390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021390"/>
    <dbReference type="HOGENOM" id="CLU_126684_0_0_1"/>
    <dbReference type="OrthoDB" id="2195638at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013252">
        <property type="entry name" value="Ndc80_Spc24"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08286">
        <property type="entry name" value="Spc24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0137">Centromere</keyword>
    <keyword evidence="1" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="1" id="KW-0498">Mitosis</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="183" mass="21437" checksum="2F678B17D92C7BE3" modified="2012-10-03" version="1">MEETKTLLQDLCEKFKNPAEKNILMALDSQRKEERMKMETVTKALQDNVQLFKKKNIQLEGEVRKYSYTHSKKNDAFIEINNEKLKLAKKIVELEDENEKIKVGIIMADKSIQEKEERLRTLSRPSFNEIYLEIVKGFGIEFLEGDGRKYCRIKNKKMSDVFTIDVGSSASMFEITNSIWEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALX5</accession>
    <name>I7ALX5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">EPP transporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_030280</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264146.1">
        <property type="nucleotide sequence ID" value="XM_009265871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALX5"/>
    <dbReference type="GeneID" id="20520939"/>
    <dbReference type="KEGG" id="ero:EROM_030280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030280"/>
    <dbReference type="HOGENOM" id="CLU_000604_57_6_1"/>
    <dbReference type="OrthoDB" id="2196280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF139">
        <property type="entry name" value="PROTEIN SCARLET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="442"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="550"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="5"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="591" mass="66572" checksum="6FCF3E4D6823B9D1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM58</accession>
    <name>I7AM58_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleoporin Nup54 alpha-helical domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_031470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264266.1">
        <property type="nucleotide sequence ID" value="XM_009265991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM58"/>
    <dbReference type="GeneID" id="20521061"/>
    <dbReference type="KEGG" id="ero:EROM_031470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031470"/>
    <dbReference type="HOGENOM" id="CLU_1299695_0_0_1"/>
    <dbReference type="OrthoDB" id="6162375at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="25136" checksum="EBA1593338C1E65B" modified="2012-10-03" version="1">MEYKIQKDQELLTLIDNIEKSYDPSSPNYKFKYVFYNKVDGPFGRPLDFPETLWNISLTSDPTMMPVLLKGDEIEHRKSLQMDACKRINDSYDFLRKKIGGLRMRSEKLKSKIDGCLQIYRKIFGGVYNRFKKEEGKCTLLDRIYRAYLPLSKREKLCVTQNKNEVIDFLEDLRGTGEKILKEVDNALQERQKQTVILNELDKVQEPFSRSTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMJ5</accession>
    <name>I7AMJ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264431.1">
        <property type="nucleotide sequence ID" value="XM_009266156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMJ5"/>
    <dbReference type="GeneID" id="20521233"/>
    <dbReference type="KEGG" id="ero:EROM_041690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041690"/>
    <dbReference type="HOGENOM" id="CLU_2061461_0_0_1"/>
    <dbReference type="OrthoDB" id="10666276at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="13996" checksum="D4792DCE9308729A" modified="2012-10-03" version="1">MPHPFYFYYHRMNPDIPEACTTDDLLKNKDRVGIYWKTVLYCFSTPISIVLPIVVYLLAGKKTIKNNLLLRLITIFFPVHTQQRNTLTYFLILGNPNIGLYSTLFLMAFFLHSLLLLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMQ6</accession>
    <name>I7AMQ6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">6-phosphogluconate dehydrogenase, decarboxylating</fullName>
            <ecNumber evidence="8">1.1.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_050870</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10116"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="1.1.1.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.44" evidence="8"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264516.1">
        <property type="nucleotide sequence ID" value="XM_009266241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMQ6"/>
    <dbReference type="GeneID" id="20521319"/>
    <dbReference type="KEGG" id="ero:EROM_050870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050870"/>
    <dbReference type="HOGENOM" id="CLU_024540_4_1_1"/>
    <dbReference type="OrthoDB" id="434986at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00410"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004616">
        <property type="term" value="F:phosphogluconate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019521">
        <property type="term" value="P:D-gluconate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.320">
        <property type="entry name" value="6-Phosphogluconate Dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006114">
        <property type="entry name" value="6PGDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006113">
        <property type="entry name" value="6PGDH_Gnd/GntZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006183">
        <property type="entry name" value="Pgluconate_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00873">
        <property type="entry name" value="gnd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006765">
        <property type="entry name" value="PRK09287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11811">
        <property type="entry name" value="6-PHOSPHOGLUCONATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00393">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000109">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00076">
        <property type="entry name" value="6PGDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01350">
        <property type="entry name" value="6PGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="8" id="KW-0521">NADP</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 8" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase C-terminal" evidence="9">
        <location>
            <begin position="172"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="179"/>
            <end position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="452" mass="50340" checksum="A052728B571FCEF4" modified="2012-10-03" version="1">MEIGLIGLGVMGHNLALNIVSKGYKLHVFNRTSSKTDRLAEESNDVYPHYSVQDMVMGIKTSPKIIILMLTSGEVVDAFLEELGKYLGKDDIVIDGGNSSFRDTIRRNNCKFRFVGCGISGGEEGARHGPSIMVGCSKGAWEEVKGLLTDISAISASRNDRCCVWLGEDGAGHFVKMIHNGIEYGDMAVITETYLVLRSLGLDNLEISSLFDSWDRMESESYLLRISSNILRMKNEKGSVIDQIIDVSEQKGTGKETVISAMEIGEAIPAITEAVTSRMVSHMKDKRCWLSSRLKGSRGGKRIPEETIRKGFYLARIVSYIQGFNLLMKARKKYGWTYTIKDITRVWSNGCILRGKLLNVVEGLGEEREEDFELTSGFVSLYDRCSVDLKEMVLYSVENEVAIPTISSCLMYLNGMKEKEGGGNMIQAMRDYFGSHTVVMKGESQSVHIEWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMS6</accession>
    <name>I7AMS6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">E3 ubiquitin-protein ligase listerin</fullName>
            <ecNumber evidence="5 16">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase listerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_051130</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">E3 ubiquitin-protein ligase component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. Mediates ubiquitination of proteins derived from mRNAs lacking stop codons (non-stop proteins) and other translation arrest products induced by poly-lysine sequences and tandem rare codons. Ubiquitination leads to CDC48 recruitment for extraction and degradation of the incomplete translation product. May indirectly play a role in chromatin function and transcription.</text>
    </comment>
    <comment type="function">
        <text evidence="16">E3 ubiquitin-protein ligase. Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the ribosome quality control complex (RQC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the LTN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5 16"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264541.1">
        <property type="nucleotide sequence ID" value="XM_009266266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMS6"/>
    <dbReference type="GeneID" id="20521345"/>
    <dbReference type="KEGG" id="ero:EROM_051130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051130"/>
    <dbReference type="HOGENOM" id="CLU_291022_0_0_1"/>
    <dbReference type="OrthoDB" id="2191389at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990116">
        <property type="term" value="P:ribosome-associated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16491">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000038">
        <property type="entry name" value="E3 ubiquitin-protein ligase listerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039795">
        <property type="entry name" value="LTN1/Rkr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039804">
        <property type="entry name" value="RING-CH-C4HC3_LTN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE LISTERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12389">
        <property type="entry name" value="ZINC FINGER PROTEIN 294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01197">
        <property type="entry name" value="FANCL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13 16" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 15 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="998"/>
            <end position="1045"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU367090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN83044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1052" mass="121901" checksum="F151C29BDA7125DC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AND7</accession>
    <name>I7AND7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264771.1">
        <property type="nucleotide sequence ID" value="XM_009266496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AND7"/>
    <dbReference type="GeneID" id="20521582"/>
    <dbReference type="KEGG" id="ero:EROM_070230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070230"/>
    <dbReference type="HOGENOM" id="CLU_1102770_0_0_1"/>
    <dbReference type="OrthoDB" id="2192556at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="241" mass="27836" checksum="39863C11F1862740" modified="2012-10-03" version="1">MRERSHPYSLLRKKFVKTMSKEKPKIDVWTAEDRQKNRLPTRGVNIDVKDVVEERPVNSSNPPIIGEKVLIKPYKGFSMDEFLSSVLEKERGLRFNFSLINSVILQFLDHGSIRSILSIESIFRAAKELPLVLISGYIYLEKYVKKAGIVFYDCKKLIKFFFGCCLIGSKFIYDTRHPDDLFFVGISPAEIFRIEGSILSAIDYDLEISNDDIRKVILQNKASLSNKEHLNAQFNRAIRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANF5</accession>
    <name>I7ANF5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264796.1">
        <property type="nucleotide sequence ID" value="XM_009266521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANF5"/>
    <dbReference type="GeneID" id="20521607"/>
    <dbReference type="KEGG" id="ero:EROM_070480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070480"/>
    <dbReference type="HOGENOM" id="CLU_723675_0_0_1"/>
    <dbReference type="OrthoDB" id="2189704at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="385" mass="44728" checksum="1FED6151B0AA1A64" modified="2012-10-03" version="1">MRTETGGGSKKTFKTMKLGFLGLVGMVVAYYVLLFIFGIKYLDPQYYGSYFYAWRVNFADKLMTHGRYKLIKKNEHPDTEDRKRFYRISQDESGPYLIRFDRPEHFPRGHEHQFTLNFPGYEDFLKIRERFIVETEGLEEDMNLSHSDLMERMKKKKEGGGMFFADYRGKSISEMQKILFPNNNSEGNPWFTVSNILIKTISSIMSSDEEKRKGYLFDGEKIGDDLMKNIRDALEALDESAGDGDVLLSEKISDSDINKFFINNGKVSATPYETFAYSRAYYLFNFLTAGIEFKSQKLAELRSESSSDDNEFREARMDYTANLFARIFSSIYPNQHRKDVSSIGTLEKIRNYYSPKMVVDEKKEVDEADLELIQKSFSNPLRVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP21</accession>
    <name>I7AP21_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265046.1">
        <property type="nucleotide sequence ID" value="XM_009266771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP21"/>
    <dbReference type="GeneID" id="20521868"/>
    <dbReference type="KEGG" id="ero:EROM_081330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081330"/>
    <dbReference type="HOGENOM" id="CLU_190111_0_0_1"/>
    <dbReference type="OrthoDB" id="2192411at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="88" mass="10077" checksum="5A43B18AE55FE399" modified="2012-10-03" version="1">MQRNKAVPMTRSLLIIMVTFTCLIRMYGTVSFPLYAVLQCALAGLFVLSTSIYGAFRIRNGDKENKSYMERIARYDALDASRKMENQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP76</accession>
    <name>I7AP76_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_082000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265111.1">
        <property type="nucleotide sequence ID" value="XM_009266836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP76"/>
    <dbReference type="GeneID" id="20521935"/>
    <dbReference type="KEGG" id="ero:EROM_082000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_082000"/>
    <dbReference type="HOGENOM" id="CLU_1669377_0_0_1"/>
    <dbReference type="OrthoDB" id="2189647at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707873" evidence="1">
        <location>
            <begin position="17"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="156" mass="17778" checksum="87D7E7607AB1A475" modified="2012-10-03" version="1">MKLLIVLMVSCIFCKSDPINVRIVSKKDPRKYLALFNGEVRLATKEQIKSNKGCSKFIVDEVRTEILQGNRRICKKYGFSKVMSCINENKNSMWGVIIDSSKFVSFKMNEEEDCITANGPDSADILGDVLSLNKCDKNSDSQKFWIKLIVNRKARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7APC2</accession>
    <name>I7APC2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5'-3' DNA helicase ZGRF1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265166.1">
        <property type="nucleotide sequence ID" value="XM_009266891.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APC2"/>
    <dbReference type="GeneID" id="20521991"/>
    <dbReference type="KEGG" id="ero:EROM_090520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090520"/>
    <dbReference type="HOGENOM" id="CLU_140543_0_0_1"/>
    <dbReference type="OrthoDB" id="2192824at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018838">
        <property type="entry name" value="ZGRF1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10382">
        <property type="entry name" value="ZGRF1-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5'-3' DNA helicase ZGRF1-like N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="117" mass="13443" checksum="A8ACF0797FF4ED7C" modified="2012-10-03" version="1">MIRCVYTAQKSKKLKTWIDGFVEHKGKRLYLYNSDMMKIDSSLASALDNEMETYKYLVYIDSFENFDQDEVENQEQISDPSASSKGNLNKGVRGSHKEECSPSKGRSEEEIINLFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7APD7</accession>
    <name>I7APD7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Signal recognition particle receptor subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265186.1">
        <property type="nucleotide sequence ID" value="XM_009266911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APD7"/>
    <dbReference type="GeneID" id="20564296"/>
    <dbReference type="KEGG" id="ero:EROM_090720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090720"/>
    <dbReference type="HOGENOM" id="CLU_148791_0_0_1"/>
    <dbReference type="OrthoDB" id="2192681at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707910" description="Signal recognition particle receptor subunit beta" evidence="1">
        <location>
            <begin position="19"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16740" checksum="5E4FB60EEEE74CB7" modified="2012-10-03" version="1">MFFLFFVVILAILIRIAAHLFTRRSTKAIKFVGPRGTGKTRTLNALMGINGKTVPTLETYKVTYKNMVVYDVVQRDGDFFERYGIDDPLVTYFFFLGSVDDLAELPETKGFDIRFVCCKQDDCKKALGKNIIVLDKDPTQIEKYFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7API6</accession>
    <name>I7API6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CCCH-type zinc finger-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_091430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265256.1">
        <property type="nucleotide sequence ID" value="XM_009266981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7API6"/>
    <dbReference type="GeneID" id="20564367"/>
    <dbReference type="KEGG" id="ero:EROM_091430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091430"/>
    <dbReference type="HOGENOM" id="CLU_061846_0_0_1"/>
    <dbReference type="OrthoDB" id="278280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032378">
        <property type="entry name" value="ZC3H15/TMA46_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681:SF0">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12681">
        <property type="entry name" value="ZINC FINGER-CONTAINING PROTEIN P48ZNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16543">
        <property type="entry name" value="DFRP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="74"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="224"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="250" mass="29155" checksum="8195B3C548A64168" modified="2012-10-03" version="1">MAKKQQETKQKSTRDMKKELEEKAFGLKNKKQKAAILKQIESLNLKEQLEKKERMKREEKKNVPVKQVIPVGVDPKTIQCINFLNKMCTDGDACKFAHGEVKKVEKPKEENVSSGPRRICQFLIDALNSGEYSNDWKCPFPKCSDIHRLVDIKENTQVELSLEEYIELSRQSLPEKLTPLTEETFKQWKARKQKEEKEHARKVKALATGPKGVELFETRRELFKDDEEAEELDYTERCYSESEDEEEGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7APM6</accession>
    <name>I7APM6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_092050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0328 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265316.1">
        <property type="nucleotide sequence ID" value="XM_009267041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APM6"/>
    <dbReference type="GeneID" id="20564429"/>
    <dbReference type="KEGG" id="ero:EROM_092050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_092050"/>
    <dbReference type="HOGENOM" id="CLU_1948795_0_0_1"/>
    <dbReference type="OrthoDB" id="10662099at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019081">
        <property type="entry name" value="UPF0328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09591">
        <property type="entry name" value="DUF2463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="129" mass="14989" checksum="F24395AF061FB77D" modified="2012-10-03" version="1">MIFPSLFVSFSYMLSVSCNLSPGSISLVDSNINILIYILILLRTTTSLILRINRSMFYSYPIVISAFLILLRSIREKYFSSKKRTLSSISVVFILILRFLIYLITARTCLIILRNQYKIQRLRTFLDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7APY4</accession>
    <name>I7APY4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010270</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263869.1">
        <property type="nucleotide sequence ID" value="XM_009265594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APY4"/>
    <dbReference type="GeneID" id="20520654"/>
    <dbReference type="KEGG" id="ero:EROM_010270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010270"/>
    <dbReference type="HOGENOM" id="CLU_1343226_0_0_1"/>
    <dbReference type="OrthoDB" id="2188797at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707917" evidence="1">
        <location>
            <begin position="19"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="235" mass="26644" checksum="F38D1DB65A306136" modified="2012-10-03" version="1">MFLGILGSLILTLRAVRGSYQQPGTGSTSQMVVLKNDINPKPECYQIYQGPMQAPVCATFTQPVVTTGIVPQRPEKSRLSPDLVRLIQSIPGASEKLSSFLEYKGITQPWPEIFDFSSDKHYGLPAPEPMFSKIQSLLNVLEDIKELLSNELFKVETYISRGKGHLCESIKKLRRYDGILKKYLKKISSAKAAKSKHNYATKYTEFSEKAHEEAKSYYETKKWLQTIVTLFRSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ05</accession>
    <name>I7AQ05_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265456.1">
        <property type="nucleotide sequence ID" value="XM_009267181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ05"/>
    <dbReference type="GeneID" id="20564574"/>
    <dbReference type="KEGG" id="ero:EROM_101440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101440"/>
    <dbReference type="HOGENOM" id="CLU_030988_6_2_1"/>
    <dbReference type="OrthoDB" id="10249039at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBC core" evidence="1">
        <location>
            <begin position="15"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="159" mass="18258" checksum="651D3E30D7A08C9F" modified="2012-10-03" version="1">MGYKWPMCPLETSSFAAFRLKTEIKSIELLPGTSLSVDKSSEERYVMEVGYTMPITEGIYEGRVLRFKIKIPHAYPFKPPKLLCLDKVFHPNIDDDGNVCMEILRLGWRPTHGLESVFVNLYVIFVEITGEDALNTLAGDLLRMDYERFVRIAKGHEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ50</accession>
    <name>I7AQ50_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265501.1">
        <property type="nucleotide sequence ID" value="XM_009267226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ50"/>
    <dbReference type="GeneID" id="20564619"/>
    <dbReference type="KEGG" id="ero:EROM_110200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110200"/>
    <dbReference type="HOGENOM" id="CLU_010412_6_4_1"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="77" mass="8506" checksum="5C5E70F159992826" modified="2012-10-03" version="1">MQIKVKTIAGRDLELNVDPEETVLDLKLKIESIELIPAEQQRLVSNGRILMKEKDTLASLNIVSGNVVHMVLALRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQN3</accession>
    <name>I7AQN3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spore wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265671.1">
        <property type="nucleotide sequence ID" value="XM_009267396.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQN3"/>
    <dbReference type="GeneID" id="20564792"/>
    <dbReference type="KEGG" id="ero:EROM_111930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111930"/>
    <dbReference type="HOGENOM" id="CLU_1366239_0_0_1"/>
    <dbReference type="OrthoDB" id="10545169at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707883" description="Spore wall protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="22309" checksum="CCD583B352D873AF" modified="2012-10-03" version="1">MKYFKFKWAFLVQAILCSQSQDMGTFDFESPLESDPERFLGKREDELLEILKSQAESIKSAVCNIISGSWGGELPGGTSTGEGEEEEPSYYSDEHPGPYENCEWATNSLEPLSDTTTKTLPELTSGLVYITSALLSKNFEVSEFRERIDSFMRIILKKSIDEAKTEDDPNVKAIESSQAEALQAIGQIESMSSSISEIYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARY5</accession>
    <name>I7ARY5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dynactin complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_060330</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARY5"/>
    <dbReference type="GeneID" id="20521430"/>
    <dbReference type="KEGG" id="ero:EROM_060330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060330"/>
    <dbReference type="HOGENOM" id="CLU_1180209_0_0_1"/>
    <dbReference type="OrthoDB" id="2130750at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916">
        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18916:SF6">
        <property type="entry name" value="DYNACTIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0243">Dynein</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CAP-Gly" evidence="8">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="92"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="235" mass="27123" checksum="A140E9DAED3ADAC8" modified="2012-10-03" version="1">MSLLMVNDRLTLGDKFKGTVRYIGKIKSKDGKWIGLELDEPVGANNGSVNGIRYFHCKDKHGIFIRYEKIREGLVCESRGVSADGKISRDQTHLYELKIKKLEETIESLKSTEKEEIVELRKENEEIKRIVLSLREGINSKKIDRDGRIYLELKELVEGSRKHISDMVGLVSDIDEILNRNKLANRKSVQECERHRVMFLVKRIIDSVLDDNIEAAENFKGEFVNIMKKYGINVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS00</accession>
    <name>I7AS00_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_060430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS00"/>
    <dbReference type="GeneID" id="20521440"/>
    <dbReference type="KEGG" id="ero:EROM_060430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060430"/>
    <dbReference type="HOGENOM" id="CLU_173461_0_0_1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="7"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="110" mass="13011" checksum="FDE66D70BA0B8D82" modified="2012-10-03" version="1">MNRKPKREGKKPRTRMTGDQIKALMACFQDNPFPSTSTREELSRILSISPRTVQIWFQNQRQRIKNQMRMISEGEVFEENVHKECPGKLCILAHVATRRMEERHELDLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS60</accession>
    <name>I7AS60_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="2"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264739.1">
        <property type="nucleotide sequence ID" value="XM_009266464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS60"/>
    <dbReference type="GeneID" id="20521548"/>
    <dbReference type="KEGG" id="ero:EROM_061510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061510"/>
    <dbReference type="HOGENOM" id="CLU_013572_0_0_1"/>
    <dbReference type="OrthoDB" id="273917at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010605">
        <property type="term" value="P:negative regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043634">
        <property type="term" value="P:polyadenylation-dependent ncRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054708">
        <property type="entry name" value="MTPAP-like_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002058">
        <property type="entry name" value="PAP_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045862">
        <property type="entry name" value="Trf4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092:SF15">
        <property type="entry name" value="INACTIVE NON-CANONICAL POLY(A) RNA POLYMERASE PROTEIN TRF4-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23092">
        <property type="entry name" value="POLY(A) RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22600">
        <property type="entry name" value="MTPAP-like_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03828">
        <property type="entry name" value="PAP_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poly(A) RNA polymerase mitochondrial-like central palm" evidence="7">
        <location>
            <begin position="43"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="PAP-associated" evidence="6">
        <location>
            <begin position="238"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="355" mass="40786" checksum="FC0484BDCA75A058" modified="2012-10-03" version="1">MSIKPTRDSKARIGSKDHAPSSLGSLLDTSKSSVSLGNLEKLDLELLQLYQELAPTQIEINSRTYIFERIKKVIVREFPNADVMPFGSHTTGLIIPSSDIDINIQLGTNTDKEYSNKYLSKIRSLMMGVSFIKKETLFHIKKCKIPILKFRDRIFGFRIDISVNQPNGVEAAKFVRYTLKEYPYMRVFAILLKHFLTIRNQSDAATGGLNSYSQFLLLLSFFQLHPLVQEGQVSPLKNIGVLFMDFFQYYGCSFPYKIARISVNKVGYVKNNTKTLSIEDPTDPECDVAAVCRNSQLILEIFRYAYRSMNAALKMKIPAQKSLASLWFRKETESIRQREEIGRIYRKKILKHHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT27</accession>
    <name>I7AT27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_081930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265104.1">
        <property type="nucleotide sequence ID" value="XM_009266829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT27"/>
    <dbReference type="GeneID" id="20521928"/>
    <dbReference type="KEGG" id="ero:EROM_081930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081930"/>
    <dbReference type="HOGENOM" id="CLU_717680_0_0_1"/>
    <dbReference type="OrthoDB" id="2194875at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707920" evidence="2">
        <location>
            <begin position="17"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="378" mass="41358" checksum="FD129C8DC723B6EC" modified="2012-10-03" version="1">MRGAVVVLFIASRTVCDVIRLVPKCDSACMKGRLLASYYASLVPMPPVYLSATDSPVAKKPRIVRPAVVNLDGVKRSISKPRLAKVPEVVKTTVTATVLKAVPMEMPRTVQPPIVVMQPRYVMQPIPVALSPMSVSTVPKPKQSVRPSRPWIAVQQQGSGAEAINRIGKRVNLIYTMLTTLKQKYLQMIPPRPQRIVVVAPVTTMTILKTVSITPPQRTEIPVINQPITVVTLTKSASQPPQASKSVKVQPSTSTACVPSSQSTKGSSKVVKSNKNTESDKDTQRAQKRDSEDSSSSQESSSSSEKGGKPLYMLKSARLGRKRPKKRESFIDCYLRGNPSECTAENSEGRLDIGDAVIYRNDFDSDDEVIYLSDLLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT96</accession>
    <name>I7AT96_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265139.1">
        <property type="nucleotide sequence ID" value="XM_009266864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT96"/>
    <dbReference type="GeneID" id="20521963"/>
    <dbReference type="KEGG" id="ero:EROM_090250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090250"/>
    <dbReference type="HOGENOM" id="CLU_641242_0_0_1"/>
    <dbReference type="OrthoDB" id="2186859at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031543">
        <property type="entry name" value="DUF5087"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17006">
        <property type="entry name" value="DUF5087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="400" mass="46657" checksum="A5326207656CCAB6" modified="2012-10-03" version="1">MIKSFEEALLRISELEEKTENQQIIIDALVAHTKNLMKGDNGVALKKDCVEQEGSESGRTVAQDMARFELKRGEDRKSRRSSKSLEKEVEDLFRRDKTSKQFKDAFTIDSLLSYSKEAHSFSLNVRTEVRMKKSFHTNVKQQIDKIIRHMIVNLNTYDLNTVCSTLNLISGDIEYQHKLVITHDIVLFIDDFSRIPFIVSALFNNQGIYDDVLGSTIKEILFHQCMVDSDINRSHPHIVGYYNKIIDNFELHPKEKSLKDLCANLIGNFNVFEDGGFNPAIFPSMYSVRVLCHYMDWDYTYNEFICSVLYPKLRETRNPLYAIYMFTAVFNALRVFGYIESVELVFSRLKELMDDSSDLSIVSYLFIKQVDPERADRWYESQRGNIRHVDGEYLRNLLLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATE3</accession>
    <name>I7ATE3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265199.1">
        <property type="nucleotide sequence ID" value="XM_009266924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATE3"/>
    <dbReference type="GeneID" id="20564310"/>
    <dbReference type="KEGG" id="ero:EROM_090860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090860"/>
    <dbReference type="HOGENOM" id="CLU_969864_0_0_1"/>
    <dbReference type="OrthoDB" id="2190279at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="284" mass="32083" checksum="E285FFD1C8C6A0AE" modified="2012-10-03" version="1">MVSFDSTVLVETDAKRDFKLPHTRQMILIETVFAIQSSPFPKYLYVYDKGFTVYQLAPFKPIKTFGYSGEVIDVGIIKGKMAILSKDSIIVYNRDEDYVVTGKFDGRLSEVDGKLGLQERAKLTLYSLDSLNVESVYKANAHYSVRDVLVTSFDNTVSLYCKGKKLIEISMPERICRVCTDPLLTNIYCGSSDGTIFCCNTSYMEPVTMRYHKSKIVGLDISFCGRHLYSADMEGVVCIWDIKFNVVVGKVSMETAIRGIKVAYVSEWKGELDAMESELVEIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATR4</accession>
    <name>I7ATR4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_100560</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265369.1">
        <property type="nucleotide sequence ID" value="XM_009267094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATR4"/>
    <dbReference type="GeneID" id="20564486"/>
    <dbReference type="KEGG" id="ero:EROM_100560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100560"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_1"/>
    <dbReference type="OrthoDB" id="7396459at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17960">
        <property type="entry name" value="DEADc_DDX55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="35"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="226"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="460" mass="52042" checksum="609DB5206EF11896" modified="2012-10-03" version="1">MYVKGVEDVCMNSRLKKEVGANGFGEMTDVQRMCVPEMLKGKDVVVQSPTGTGKTMAFLIPILSCIYDGKGSGNLDVVAMIITPTRELAMQIKEVAELFDVRCECFIGGMNIEKDYERMKGEFSIVVGTPGRLLEIVSKDIKKFSRIRHVVLDEADKLLGFGFEEKLMQLIKKLPKNRVTGLFSATINDSVDKLSRASLRNPVSINVGNSAMPVALEYVILSPMEKLFALIDIVNGRRCIIFFATCNQVDFFFGLLSRIGLENIYKIHGKISQDERNKVYKEFCQGNSLLFCTDVAARGIDFRDVDLVVHFDVPKEYSNIVHRSGRTARNGCKGESILFVMPNEKAYTEFLKLKGIPVVESSYRMKPTISYDDVKSKIDSNLLSLSVKAFVSYIRSYKEHFASYILDYKGLDFDSLAELFFLEKIPGMAELRNVRFAKFAKPPEGGRKREKPKRKYKRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATU5</accession>
    <name>I7ATU5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CLASP N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265404.1">
        <property type="nucleotide sequence ID" value="XM_009267129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATU5"/>
    <dbReference type="GeneID" id="20564521"/>
    <dbReference type="KEGG" id="ero:EROM_100910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100910"/>
    <dbReference type="HOGENOM" id="CLU_446242_0_0_1"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="479"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="639" mass="71371" checksum="D448CA9E37711E05" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU13</accession>
    <name>I7AU13_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="8">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_101570</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="domain">
        <text evidence="8">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="8"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265469.1">
        <property type="nucleotide sequence ID" value="XM_009267194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU13"/>
    <dbReference type="GeneID" id="20564587"/>
    <dbReference type="KEGG" id="ero:EROM_101570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101570"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_1"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="32"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="224"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="450" mass="51792" checksum="91BECBFCB211A9A5" modified="2012-10-03" version="1">MKFSDLNIDQRIEKGLNENGFITMKEIQQRVIPVALEGNDIIGSSQTGTGKTLAFLIPILQRLTDLQWRREDGLGCLIVTPTRELALQIFDVLTRVGKHTTLNAGLIMGGVEAENESLKISTMSILICTPGRFLQHLQENPCLKTENIQILVLDEADKMIEMGFKEILEDILEYIPSKKQTLLFSATPKASTARILKLEDPKIISIYKEEGFPSRLHQYFYMMRIEDKISYLHTFIRSNPEVKGIVFFSTCKEVKFHHLLFERLKLRNRVFCLSGGISQRQRIDTFKRFVKEKNGILFCTDLGSRGLDFPKVDVVIQYDCPCNVETYVHRVGRTARNNEGGESYVYLVYGEERLLGDIQKKGWIRSQEAKDKKAEDESISEEIQEGPKIQIKCIDKMIKGLVKSNKELNEYCKKYLVTYEKFLGFSSKRYSESILRKIPSLYEYFGISKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUD9</accession>
    <name>I7AUD9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RuvB-like helicase</fullName>
            <ecNumber evidence="10">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_111160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="10"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265594.1">
        <property type="nucleotide sequence ID" value="XM_009267319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUD9"/>
    <dbReference type="GeneID" id="20564715"/>
    <dbReference type="KEGG" id="ero:EROM_111160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111160"/>
    <dbReference type="HOGENOM" id="CLU_028311_4_1_1"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 10 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="7"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN84097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="369" mass="41140" checksum="90EC6384C1B9BB8B" modified="2012-10-03" version="1">MVESNKGGKIVLIKGERGSGKTALAIGLSKSLGGAPFNSISGTEIYSLEMSKSEAITQALRKSVGLRIKESVKVIEGEVVSLSGRRIVLKTVDMESSFDIGEKMRNELDKEKVSTGDIVRIVRERGKVYKIGTSVVKKTEVVGTDTRFIPCPEGELIRIREEVQEISLHDIDVVNNKAEGYLALFSGETGEIRAEIRDEVNKKVWNWINEGKAEVVRGVLFIDEVHMLDIESFAFLNKAIEEDFCPVILISTNKKECIIRGTDEISPYGLPRDFIDRALIISMEKYCKRDLEGIISHRILEEDIHIDSKAMDKLVSISEVSGLRYSMNLLTISGLRASKRGGKVTLEDIERTFELFLDEARGVENLTAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUM6</accession>
    <name>I7AUM6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kinesin-like DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111910</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265669.1">
        <property type="nucleotide sequence ID" value="XM_009267394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUM6"/>
    <dbReference type="GeneID" id="20564790"/>
    <dbReference type="KEGG" id="ero:EROM_111910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111910"/>
    <dbReference type="HOGENOM" id="CLU_001485_27_3_1"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115:SF1000">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24115">
        <property type="entry name" value="KINESIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0505">Motor protein</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="269"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="286"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="475" mass="53556" checksum="A8CDE861E67517BC" modified="2012-10-03" version="1">MKGHNQTLFCYGSTGAGKTYTMVGHSGSYGLMHNLVKDVLAYGSFLVSYMEIYNEKIYDLLEPKELVLRECNGTIVIPNLFNKRVENIEEFEEVFRQGTKNRTTAETKLNKNSSRSHGILRISVGEHKLNLIDLAGSENNRKTGNEGIRLTESNSINRSLFVLGKVVNAILKGEKRIPYRDSKLTRLLQDSLGGSSLCYIIANIVGDSSGMGDSINTLSFASKSRNIVNILDVAQKPRGKDFKNGGCSLSELGKRKPLSIKTNMKFNIQRKEDMPLRSKEKENNPHQESFPTSTSAIGGKKKGKSSLIYRRCKEDGGKFSKNPKLTSNGSSLILKSLLEKPDIVLSPRTKEKSYRAFLKRAQEFESAKKYRLALEDYKTMQKFCDNDFIKQKIEKINMLIKKTRRKPKVTKENVLQIINQGNFFDIKKLPRVGDKRAQAIVDFVNGGNFFETLNDLKLLFSEKIVDSILFSIDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH39</accession>
    <name>I6ZH39_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_020330</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the CEF1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN82508.1"/>
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    </dbReference>
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    <dbReference type="GeneID" id="20520791"/>
    <dbReference type="KEGG" id="ero:EROM_020330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020330"/>
    <dbReference type="HOGENOM" id="CLU_090754_0_0_1"/>
    <dbReference type="OrthoDB" id="1410009at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11659">
        <property type="entry name" value="SANT_CDC5_II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CDC5L/Cef1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
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    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047240">
        <property type="entry name" value="SANT_CDC5L_II"/>
    </dbReference>
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        <property type="entry name" value="CELL DIVISION CYCLE 5-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="9">
        <location>
            <begin position="54"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="10">
        <location>
            <begin position="62"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010506"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="170" mass="19413" checksum="5F99B2E4BC8897A5" modified="2012-10-03" version="1">MKLEKVDVSWTKTEDEILRAGVMKYGINKWSKVSSLLPSKTPSQCRLRWQEYANPLANNSEWSAEEDEKLILTAKTFHPQWSLIGQALGRNGQQCYERYNELVFGKIDVFKYGELENASEGGDDEILKMANARMSGCKNRKDLKKKILLNKKGKVSSRNSSEEGPNHNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHK7</accession>
    <name>I6ZHK7_ENCRO</name>
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        <submittedName>
            <fullName evidence="6">Splicing factor 3A subunit 2</fullName>
        </submittedName>
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    <gene>
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    </gene>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="10250970at2759"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052092">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="96"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="180" mass="21088" checksum="6453EE70B1084E8A" modified="2012-10-03" version="1">MGLRMGNAHTASEIKKHKRERSRRLFLEAYGLTADQNLSKDSVGRYICIICKTKHLTEMSYVKHREGKKHREKLSAKEEVKSNIPTHNTRCLVKGDRKGYGIVIDYKLAEEMPQYRFVNSLEQAVEDYDECFGYLVFICKPYENVGFKFESKKVDKDSIYEDIDEETGTYTFHFYFLEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHM4</accession>
    <name>I6ZHM4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Splicing factor CC1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030620</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264180.1">
        <property type="nucleotide sequence ID" value="XM_009265905.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20520973"/>
    <dbReference type="KEGG" id="ero:EROM_030620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030620"/>
    <dbReference type="HOGENOM" id="CLU_1019525_0_0_1"/>
    <dbReference type="OrthoDB" id="275748at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF119">
        <property type="entry name" value="NUCLEAR RNA-BINDING PROTEIN SART-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="67"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="151"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="226"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="273" mass="31156" checksum="6B9D81B5D47EA62D" modified="2012-10-03" version="1">MKKTVEKVNKNSSESESGSGLSETEESSSNGVGQANCSESGSEYESEIEEETSSGTEEGTKKENPSLTVFIKGISYDLTEYDLKSEMEKIGKVARVGIPMTNDYKRNKGFGYVEFCKEEDVKKALKLDGTVFLGREVVVNMAHPRENKQRHTIYISNIPFGCDKRKLKKYFEEMGEVVGVSFPYDKENDRLKGYGFIDFGNKEDYEKVLKKKLVFEDSSIYQRPAYKNKEDRKDFNGKGPRRNDSRWNGKKYGGESQNRNNNKKVKFNSDSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHX4</accession>
    <name>I6ZHX4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_040450</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264310.1">
        <property type="nucleotide sequence ID" value="XM_009266035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHX4"/>
    <dbReference type="GeneID" id="20521109"/>
    <dbReference type="KEGG" id="ero:EROM_040450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040450"/>
    <dbReference type="HOGENOM" id="CLU_119261_1_1_1"/>
    <dbReference type="OrthoDB" id="201213at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042237">
        <property type="entry name" value="PTRHD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46194">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46194:SF1">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE PTRHD1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="114" mass="13299" checksum="EE0BA87B014ED9F6" modified="2012-10-03" version="1">MAIVQYILLRNDLNFSRGALIAQACHSSVYSIERYRDHPDTVEYLRNVRLMTKVVLKIKGKDVGEIIDHLVSKNIDHTVWVEEPESITTCISLRPYAKDKIADVVEYFKRFKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIG0</accession>
    <name>I6ZIG0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ca2+-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264505.1">
        <property type="nucleotide sequence ID" value="XM_009266230.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIG0"/>
    <dbReference type="GeneID" id="20521308"/>
    <dbReference type="KEGG" id="ero:EROM_050760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050760"/>
    <dbReference type="HOGENOM" id="CLU_149442_1_0_1"/>
    <dbReference type="OrthoDB" id="26525at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13710" checksum="FCEEB1EE5BC8868F" modified="2012-10-03" version="1">MERDSLQNDPRAFDIGKKGFLSYEEYKGYCLSILKQPLGRKKTGDRIEYNDIRFESCGAEIDGVFDFFSSGEDYISFQTLKKAISKLEMSISNEDIAMMLDMLDSNKQVSRELFSRLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIH3</accession>
    <name>I6ZIH3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264520.1">
        <property type="nucleotide sequence ID" value="XM_009266245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIH3"/>
    <dbReference type="GeneID" id="20521324"/>
    <dbReference type="KEGG" id="ero:EROM_050920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050920"/>
    <dbReference type="HOGENOM" id="CLU_091062_0_0_1"/>
    <dbReference type="OrthoDB" id="5961at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040202">
        <property type="entry name" value="Brl1/Brr6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018767">
        <property type="entry name" value="Brl1/Brr6_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136:SF1">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28136">
        <property type="entry name" value="NUCLEUS EXPORT PROTEIN BRR6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10104">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01042">
        <property type="entry name" value="Brr6_like_C_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Brl1/Brr6" evidence="2">
        <location>
            <begin position="79"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="209" mass="24246" checksum="7F399C5232795828" modified="2012-10-03" version="1">MHGEIPMDVEHELGWKRESKQRGGGCSHLSPLKKRKIGAETAKESCTQMVKCLNDRRKVATKKVKWIYAINISRLPMTIIGYLHLVLNFVVIGFVCYVIMYVLIGLERDIRYKIINRRTQIRHQIEEAKTFYKVNKCDPATRVPAIEDLCNKWECVIKNGYWSIGYTRIIAEVFGDVMDGFIRKFSIRSSLTIGFFFVLFLVFRRASSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIL0</accession>
    <name>I6ZIL0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">G10 cell cycle control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_051270</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BUD31 (G10) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264555.1">
        <property type="nucleotide sequence ID" value="XM_009266280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIL0"/>
    <dbReference type="GeneID" id="20521359"/>
    <dbReference type="KEGG" id="ero:EROM_051270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051270"/>
    <dbReference type="HOGENOM" id="CLU_087132_1_1_1"/>
    <dbReference type="OrthoDB" id="277109at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001748">
        <property type="entry name" value="BUD31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411:SF0">
        <property type="entry name" value="PROTEIN BUD31 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19411">
        <property type="entry name" value="PROTEIN BUD31-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01125">
        <property type="entry name" value="BUD31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00322">
        <property type="entry name" value="G10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="140" mass="16458" checksum="03CFFDE99C80825D" modified="2012-10-03" version="1">MPRIPVNPPKEFEKVRTFLENMEAEMRKAENAPTVPNPPENYWPIFQLHHQRSRHIYNLKKKGEISNELYRYLSLNQFVDHELVCYWEREGYEGLCCLRCVQPIDSKHGNVCICRVPQRNIDVNKAIKCDNCGCRGCSGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIN9</accession>
    <name>I6ZIN9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Casein kinase II subunit alpha</fullName>
            <shortName evidence="8">CK II alpha</shortName>
            <ecNumber evidence="8">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_051580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="8"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264585.1">
        <property type="nucleotide sequence ID" value="XM_009266310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIN9"/>
    <dbReference type="GeneID" id="20521390"/>
    <dbReference type="KEGG" id="ero:EROM_051580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051580"/>
    <dbReference type="HOGENOM" id="CLU_000288_70_4_1"/>
    <dbReference type="OrthoDB" id="10254671at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14132">
        <property type="entry name" value="STKc_CK2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000059">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000088">
        <property type="entry name" value="Casein kinase II subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045216">
        <property type="entry name" value="CK2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24054:SF0">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="37"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="320" mass="37798" checksum="7BE1A77C4352D01C" modified="2012-10-03" version="1">MVVVTIAKSNADANEKKDEKYWNYECYNIPTGCIEKYQIYQRMGRGKYSEVFEGRKDKEKIVIKTLKPVRKAKISREVLILKSLSHKNIIKLIDVVVDSESQIYSLIFEYIEHEDYAKIFEKLSYMDIIEYSKQILSALDYCHSMGIIHRDIKPQNMVINRERRELKIIDWGLAEFYHPKKEYSVRVASRYYKSPELLVDYPYYDYSLDIWSFGCVLAELIFKKRPFFHGESNSDQLVKIARVLGYTDLRKYIEKYRIAPLSPKHEGTGERVLLSSFVPLGKANLYIHAIDLLEKILVYDHQSRPTAEECLRHPLFKLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIQ2</accession>
    <name>I6ZIQ2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein kish</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_060080</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the KISH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264600.1">
        <property type="nucleotide sequence ID" value="XM_009266325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIQ2"/>
    <dbReference type="GeneID" id="20521405"/>
    <dbReference type="KEGG" id="ero:EROM_060080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060080"/>
    <dbReference type="HOGENOM" id="CLU_152663_1_0_1"/>
    <dbReference type="OrthoDB" id="10034655at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051523">
        <property type="entry name" value="KISH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13229">
        <property type="entry name" value="PROTEIN KISH-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU910717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="74" mass="8186" checksum="5B526B9E315687CA" modified="2012-10-03" version="1">MSALFSIDSLFRALILAICTATYLKQHFPSLVSKNKKGFYSVFHKLSVVGERLSPFVALSCIFLGIGKITSIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIY0</accession>
    <name>I6ZIY0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-directed RNA polymerase II subunit Rpb3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264645.1">
        <property type="nucleotide sequence ID" value="XM_009266370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIY0"/>
    <dbReference type="GeneID" id="20521451"/>
    <dbReference type="KEGG" id="ero:EROM_060540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060540"/>
    <dbReference type="HOGENOM" id="CLU_038421_1_0_1"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="4">
        <location>
            <begin position="13"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="240" mass="26664" checksum="452C66685F43FEFE" modified="2012-10-03" version="1">MIGLTVEELTPERIKFTLSNATVGYANALRRILISEVPTIAIDMVEIERNNTVLPDEVLAHRLGLVPIYSKRELKYKEECSCSGFCSECSVVFEIDIVHTEDALRVVSARDIVCDEGDVVIKSGPMITKLARDQGLKARCIGRKGIGKIHAKWSPVSAISFEYDKDNVRRETNYWFEESIEEEWPGVKQGEANPMQKVDKIHMCVEVIEGGAGPIAIVLEGLKILKEKIAALASATETMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIZ4</accession>
    <name>I6ZIZ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264665.1">
        <property type="nucleotide sequence ID" value="XM_009266390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIZ4"/>
    <dbReference type="GeneID" id="20521471"/>
    <dbReference type="KEGG" id="ero:EROM_060750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060750"/>
    <dbReference type="HOGENOM" id="CLU_2096859_0_0_1"/>
    <dbReference type="OrthoDB" id="114080at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="122" mass="14164" checksum="9182B257B81D9FFC" modified="2012-10-03" version="1">MGLETRTSEEENLKLMEELEILKLVVYKSKNGHRGSKLFRKLVHLKRLSQSFLLNKVKSKKDEIRRVSEELYILATSNIPEGHLISYTLIILGLCSRIHYLVGDIECIEDTNDIDEMFAEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ07</accession>
    <name>I6ZJ07_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Apoptosis-antagonizing transcription factor C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264685.1">
        <property type="nucleotide sequence ID" value="XM_009266410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ07"/>
    <dbReference type="GeneID" id="20521492"/>
    <dbReference type="KEGG" id="ero:EROM_060960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060960"/>
    <dbReference type="HOGENOM" id="CLU_1686552_0_0_1"/>
    <dbReference type="OrthoDB" id="2190036at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012617">
        <property type="entry name" value="AATF_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08164">
        <property type="entry name" value="TRAUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Apoptosis-antagonizing transcription factor C-terminal" evidence="1">
        <location>
            <begin position="79"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="156" mass="18181" checksum="F236D7568D872139" modified="2012-10-03" version="1">MESLKITVKELLRKTESQIDKIAFLHANPTQAREIKRTIDTLDNPQELLRRCIARMDKLNSLMNPMKHTISTIPSIEEQLKYLYERKKAFDENKVYTLLAKNKVNVMLSNKKAAFKSKGKTLSYEFKEKLTGFLTRRGCASWSDEKIDDFISSMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ15</accession>
    <name>I6ZJ15_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">30S ribosomal protein S13P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_061060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264695.1">
        <property type="nucleotide sequence ID" value="XM_009266420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ15"/>
    <dbReference type="GeneID" id="20521502"/>
    <dbReference type="KEGG" id="ero:EROM_061060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061060"/>
    <dbReference type="HOGENOM" id="CLU_103849_0_1_1"/>
    <dbReference type="OrthoDB" id="1702480at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000001">
        <property type="entry name" value="30S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="153" mass="17085" checksum="BFADBB4830EDE82F" modified="2012-10-03" version="1">MNYTYDPAEVQYIIRIHNTNIDGTKRIAFALTGIRGVGIRISTAICKRLGIDLKKRAGEMSEEDMKRIGDAILDPASVGIPESYMNHQKDVIDGTTSHLIGTKLDADLRMMIERGKKNKLIRACRLDVGLKVRGQRTKSNGRRGKSMGVSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ31</accession>
    <name>I6ZJ31_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_061330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264720.1">
        <property type="nucleotide sequence ID" value="XM_009266445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ31"/>
    <dbReference type="GeneID" id="20521529"/>
    <dbReference type="KEGG" id="ero:EROM_061330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061330"/>
    <dbReference type="HOGENOM" id="CLU_129408_0_0_1"/>
    <dbReference type="OrthoDB" id="2196113at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="36"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="175" mass="19811" checksum="4483929583DD5669" modified="2012-10-03" version="1">MKYLPIFVAFVVAIPEDLNSIIMAINRLHKNTKYSTYYEVFGVSENASIASIKKLYQKMMKHPSPIPGIEKREDAVALLTEAYNILKNKKSAYDFVLANSYIYIGGKNNFKNNLYVILMSISVGLLAIDLIYFGVKYLVFMANPKKASKKKGNKESVPSMMIVSILRLIKSLIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ39</accession>
    <name>I6ZJ39_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Translation elongation factor 1-alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_061420</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264730.1">
        <property type="nucleotide sequence ID" value="XM_009266455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ39"/>
    <dbReference type="GeneID" id="20521539"/>
    <dbReference type="KEGG" id="ero:EROM_061420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061420"/>
    <dbReference type="HOGENOM" id="CLU_007265_3_8_1"/>
    <dbReference type="OrthoDB" id="342024at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="11"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="424" mass="47847" checksum="5B0DD9E65E6861F4" modified="2012-10-03" version="1">MDLEEMRISRKKVINIVFVGHVDAGKSTICGQILVQMGLVDPRTLEKYKQMSREQNRESWYLSWCLDINPEERERGKTTEVGTASFELPHRRINILDAPGHNQFVFEMINGANRADVGVLVISARINEFEAGFEKGGQTREHIFLLKAGSVQRLIVLVNKMDDSSVEWSKKRFDEIKDKVGSFVKRMFPSPVFIPVSGFTGEYIKEKGTCSWYDGDSFLSELDKISIPRRFDAPLAITVTEKVKVMGSMVLFGKIECGKVVPGMPVKILPQNTRNVILSIMDEEEVEIEEGEPGDVVKLKLKEEADDVLVGSKILDTDSTDYKSTQEFTCGLNILDSDTIISSGYTCILHVGIVTVQCKIKEIRDINNKKIRFCRHGSRVLAKIVTPSPICVFYGSRDEEERQRFALRLESKTIAVGVIRAIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJF9</accession>
    <name>I6ZJF9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Eukaryotic translation initiation factor 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_071270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264875.1">
        <property type="nucleotide sequence ID" value="XM_009266600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJF9"/>
    <dbReference type="GeneID" id="20521687"/>
    <dbReference type="KEGG" id="ero:EROM_071270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071270"/>
    <dbReference type="HOGENOM" id="CLU_088630_0_0_1"/>
    <dbReference type="OrthoDB" id="10250831at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071074">
        <property type="term" value="F:eukaryotic initiation factor eIF2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF7">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 8" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="7">
        <location>
            <begin position="12"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="280" mass="32491" checksum="ECAF251835A9BFD2" modified="2012-10-03" version="1">MIDISRTNNDFYYRYKMPRAVVKLEGKAGNTRTVIVNLEEIGSSLKRPPLYILKFMSYELATRIDVVKGRYAVNGRYDSSRVQDLIYDFIDRFVMCPFCNNPETFYVNNDGLSMECLACGKRSGVKASKLSGMILKDVEKNSSGHDDTYFNPVGPEDDEYKDNMRRLMESDDDRSEDIVNLLKDHGLSDEKIAKEVLMFDGGIKKCKRINDFISPKAFLSSVEEVAENGKEKNIQEYLRMLEEEKMFKRSELFKYFTRPQGNKKRSPEFKKEISDYFSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJI5</accession>
    <name>I6ZJI5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Threonyl-tRNA synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_071520</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264900.1">
        <property type="nucleotide sequence ID" value="XM_009266625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJI5"/>
    <dbReference type="GeneID" id="20521716"/>
    <dbReference type="KEGG" id="ero:EROM_071520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071520"/>
    <dbReference type="HOGENOM" id="CLU_056396_0_0_1"/>
    <dbReference type="OrthoDB" id="2187573at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707021" description="Threonyl-tRNA synthetase" evidence="3">
        <location>
            <begin position="19"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="308"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="407" mass="46022" checksum="99DBE3F354F922DE" modified="2012-10-03" version="1">MRYTLLLLFRYAFSSVSTDTSSDPKKGVIEKANGEIAYHPGGSNEKVGVKGTHVESDGLSLRRHTDEGGLVVFLAPNPYEEWSFSYTFTNAKLRFPHIGGVYLWYTSDIQDQGVYRGGHDMFEGIMAGLEFRGSQPEIVMAINDGKTSLIGSEEMTLYRDTINPERLKGVKDITVKVISTEKNFKVELYDGDRLLYDSFRYYRKGTLAGIGSGKYFNITSFYDKAPSDSVYKLKEAQLFERTETSEYEVHRVHAPPVDKTPRSPDGILHEDEEIRHLISKIEYLNEYLHLVVGEPHKSSFDKVVYVLSEQLKTHMKNIEDAITSAKNKGGSEMKFMNLSEKMNGIDIKLQRIQKSFADLEHVVNSLKNDYSRSSNILVYIIVGIGAAGFGLVAMKEYQALKLRQKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJK2</accession>
    <name>I6ZJK2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_071720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264920.1">
        <property type="nucleotide sequence ID" value="XM_009266645.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJK2"/>
    <dbReference type="GeneID" id="20521736"/>
    <dbReference type="KEGG" id="ero:EROM_071720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071720"/>
    <dbReference type="HOGENOM" id="CLU_577498_0_0_1"/>
    <dbReference type="OrthoDB" id="2195860at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012468">
        <property type="entry name" value="DUF1686"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07937">
        <property type="entry name" value="DUF1686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="438"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="473" mass="53768" checksum="FF3BB5AEC19F2B27" modified="2012-10-03" version="1">MNTQKDITDYIQNIEKKLGEIVSSEASKKLLSAEEWFRERKIKTDQRNVDGQFSKVIKSIRNLMKEAKELLESIRNGEFSLYRKDIFNKYNKEIKKVKNALETYKDNICSYRDSKHYCQGKTKVGVSCVDQRKQIASTCLSVAVDLENTFVKPDPDTDISTTQTDVYEFLVQKYLLSNPKSTESQHNSSLSKTEPTYCISEGSIESPPLKRKKSASCISEKIGNPGILKVTESPGIVNGATNFKVFKKDYFLLLFCIISLGLMAFLGITKKVEASEKILGIAKKVAYTISILGPILHGRWILTNEDYNRGIVEVLSKNWNVIGSMFPMLLVLKKDSLRKTFEGKASVESYGAMVGMSVIMMCSQTFSKRDRKIHGRQMNVFVIGNILMGMAYVNNFIALSARSGHNLVLFGHIIFGLILCFMIIIEGGDRMEDTKSKGMEWTVIFQWIMSWIFVIFGFQNLESCYFHYTKNTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKY1</accession>
    <name>I6ZKY1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265475.1">
        <property type="nucleotide sequence ID" value="XM_009267200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKY1"/>
    <dbReference type="GeneID" id="20564593"/>
    <dbReference type="KEGG" id="ero:EROM_101630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101630"/>
    <dbReference type="HOGENOM" id="CLU_088310_0_0_1"/>
    <dbReference type="OrthoDB" id="267397at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015496">
        <property type="entry name" value="Ubiquilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677:SF3">
        <property type="entry name" value="FI07626P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10677">
        <property type="entry name" value="UBIQUILIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23195">
        <property type="entry name" value="UBQLN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="1">
        <location>
            <begin position="3"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="275" mass="31314" checksum="52F2A9094575C8A4" modified="2012-10-03" version="1">MSIRLTIRYRTDSIEAIAMMTDTLESLKLRIQEITGVPVDSQVLLFNDRILSDNNKELKELGIENDCTLHLKKKMEVKSEGKKPDFTASMMKNPLVKNFLKNPDAMRSIVEMFPGLKEEMNSNPELRMVMNSSNLQDELEMFSMNPEYMNTQLKNLDITMSKLENIPGGLNMISSMIKDVQDPLSSALKEGMGRGYRVKEGRKIDKPINEAIPGVCKEESQLVKYKDKLAELKQIGFSDTRRNFMALMACNGDLEGSIMYLQELDRKSSPTQRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL15</accession>
    <name>I6ZL15_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_110310</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265510.1">
        <property type="nucleotide sequence ID" value="XM_009267235.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL15"/>
    <dbReference type="GeneID" id="20564630"/>
    <dbReference type="KEGG" id="ero:EROM_110310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110310"/>
    <dbReference type="HOGENOM" id="CLU_019619_0_0_1"/>
    <dbReference type="OrthoDB" id="10264910at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010613">
        <property type="entry name" value="PES"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221">
        <property type="entry name" value="PESCADILLO - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12221:SF6">
        <property type="entry name" value="PESCADILLO HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16589">
        <property type="entry name" value="BRCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06732">
        <property type="entry name" value="Pescadillo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BRCT" evidence="5">
        <location>
            <begin position="272"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="399" mass="46369" checksum="89F8698C90CDEC78" modified="2012-10-03" version="1">MGSSYIKPPKKYISKDLVMRNLDLQEKEFDKLVTLCGVHPYIPKNNRKVDQGDGFYYRISDANKLLHSDVYRKLIINRDLENKKKRYTGTGFEYKIKNIHYKEYGYVDLIKSRCKGFGEAVDELNHTLSNLYLGRMLNLNDGISEVIEMFERFVAKKSLLEYSYMSKSGIYNQMTLGKVKVVWFVPYPGAELKDIVEEKKDMPKKFEWSEPNFLDFLSSSEESSSELEEREEAYNDPRKIDISLLSHSIPLLMTHCKLVLHKLEKVYESHEGGRKIFSDVKFYIESKAVEGALRLIVLSCGGSIVESPHEANIHISEKIDEMVENVAYLQPQYLFDCLNQGKRLYAESYSVGRKLPKHISPFASVDSIISKESLMTMSKTRRHKIEDLINRFEDVKYDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL24</accession>
    <name>I6ZL24_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Arf GTPase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_110410</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265520.1">
        <property type="nucleotide sequence ID" value="XM_009267245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL24"/>
    <dbReference type="GeneID" id="20564640"/>
    <dbReference type="KEGG" id="ero:EROM_110410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110410"/>
    <dbReference type="HOGENOM" id="CLU_070980_0_0_1"/>
    <dbReference type="OrthoDB" id="983479at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08831">
        <property type="entry name" value="ArfGap_ArfGap2_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF4">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="7">
        <location>
            <begin position="6"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="251" mass="28011" checksum="5228EC0BA75D269F" modified="2012-10-03" version="1">MVMNFNKEVKILRDIEENKRCADCSGPNPPWASVTYGIFICFDCASVHRSLGVKTSFVKSVNLDIWDEKEYLFMKHGSNEKFRKFLEQYGLVGREMNEIYNNNHIKRYAANVKSLVVKEMGEEAFNKAKISTIPKESKDSEGWMNRSSTNCVNRSRGPCSTTGLESTSTGSLHNSIASTLSIVGSAIFSSAKIITDKTVEYGGIVVSSTKSIIKDNSSSLASIFKKKELAPKDHIKVPEKQEISEKSGKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL67</accession>
    <name>I6ZL67_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265565.1">
        <property type="nucleotide sequence ID" value="XM_009267290.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL67"/>
    <dbReference type="GeneID" id="20564685"/>
    <dbReference type="KEGG" id="ero:EROM_110860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110860"/>
    <dbReference type="HOGENOM" id="CLU_1618991_0_0_1"/>
    <dbReference type="OrthoDB" id="2190550at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="164" mass="18791" checksum="F37A1AA8E4A5FF6F" modified="2012-10-03" version="1">MEAESTRAGEESISIENSNMESNAREKDDLCIRKVRSMDVSQVLVSQSCAARECAKDEIRRFSKVPVEVDTSGGRPLMFMMWASEEKAIHKKKHSTEASRDTFGCESCEEVFDTFKKLQIHRALHKDTSGEANPLFCPVCKKTFDERRKLMLHSRYHKIDKDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLG8</accession>
    <name>I6ZLG8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Nucleoside transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111770</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265655.1">
        <property type="nucleotide sequence ID" value="XM_009267380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLG8"/>
    <dbReference type="GeneID" id="20564776"/>
    <dbReference type="KEGG" id="ero:EROM_111770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111770"/>
    <dbReference type="HOGENOM" id="CLU_584071_0_0_1"/>
    <dbReference type="OrthoDB" id="515887at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF41">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="306"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="7">
        <location>
            <begin position="52"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="7">
        <location>
            <begin position="283"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="478" mass="54680" checksum="56529D691A767065" modified="2012-10-03" version="1">MDLLSKLNKKYLMVPKILYVAISMQYYTLHKFRSLFAKEMFGIGEKELSGVGVLLFITFFTNIFIATLNDRLGRPRILMVYLLSLSCIFFQLFYVKKYVKSIYLMFWINLFGYLGTNTPIMALLDKIILDYLAESPDVGVRAYGKQRIWGTIGYLLSIFIIERIIRGGMGTNAIDFTNLKYYSLAITIICVCLVMAFLKDSGSECKDLSLNTASEWKELMRNREYLFFIFIILLNGFTRSAMTIYLPIYTNYVLNVKPYALPTSWPTWIRNGLRILNDSPFSTITVFEISLEMGILFYFDAISRKIGLLLPLLLAQVSQVIRFSLYLVLPHTNPHVFAFCCLFEVLKGINFGLSHGSGVQLAEKMCPPHLKTTSQMIYNGTFMAVGSVTASLYFRYVFRNNGTSIPEEALGRKVESFKMFFISNILVSSIAIFLFLIKYGVGDGKLFNTLSSLKKINQKDVKKSKLFTENTKEPIIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS55</accession>
    <name>I6ZS55_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263928.1">
        <property type="nucleotide sequence ID" value="XM_009265653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS55"/>
    <dbReference type="GeneID" id="20520714"/>
    <dbReference type="KEGG" id="ero:EROM_010870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010870"/>
    <dbReference type="HOGENOM" id="CLU_690828_0_0_1"/>
    <dbReference type="OrthoDB" id="2195910at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707404" evidence="2">
        <location>
            <begin position="18"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="368"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="395" mass="44658" checksum="6D83D88D91D08F27" modified="2012-10-03" version="1">MIGQLSLVAVLWRCAEATDTGSSLYSVLPSNSPLFDQGFMGSIPQVQAISVPTSKPGQLLSFTPEGSGNGQQVAMTPMALQPPGNAQYANVCDMSEKKEQLKKCLADQLNKFKNILENCRKTLGENAKECCIDQKCLDLLNGGNTSCNEEDNGKDCKDKNCRDKIADEIVKNLNLDQLKKSLLDSLKGSLKKIGNKLGSLTSCSRGEGNCDEENNIMSPLGPGKGVPPGVLIYPIEQSKYEAITNILDRQQKFPQQRDIEQPDIQGNPNRSYDRGPREGWGREYPPRRESREGRRKRPRGTKRDSDDWEREEDTQDEWKSDQNIQSSRPYSPRKPEMPCTKEMCVDMDRYDSPSMKEECIKYCKDYRRKDTKKSKDEEKEEFSELTSDSCYDEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSH9</accession>
    <name>I6ZSH9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264058.1">
        <property type="nucleotide sequence ID" value="XM_009265783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSH9"/>
    <dbReference type="GeneID" id="20520847"/>
    <dbReference type="KEGG" id="ero:EROM_020860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020860"/>
    <dbReference type="HOGENOM" id="CLU_108611_0_0_1"/>
    <dbReference type="OrthoDB" id="2192459at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031547">
        <property type="entry name" value="DUF5089"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17002">
        <property type="entry name" value="DUF5089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="24447" checksum="50A852C6450798B1" modified="2012-10-03" version="1">MGGDVYSLDRAMKSKKKRGVSQDVIEEAERVEDQNYERAKGVRMQLDETALKQKATTEELMRQGETLENARKAAIGINTNARRGSDITEDIEREGRVFSCELPCVRAIKRWFRGDRGNIDDIVSKKQEEEGEREYVPEHVEFEEGEEYIKGQNKTDREMVGVLNTVRQIRAEADKQNKEAVRHKSIVNDISMINERSSKVIKETDQELKKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT68</accession>
    <name>I6ZT68_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">X-prolyl aminopeptidase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_040530</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264318.1">
        <property type="nucleotide sequence ID" value="XM_009266043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT68"/>
    <dbReference type="GeneID" id="20521117"/>
    <dbReference type="KEGG" id="ero:EROM_040530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040530"/>
    <dbReference type="HOGENOM" id="CLU_011781_2_4_1"/>
    <dbReference type="OrthoDB" id="9995434at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000007">
        <property type="entry name" value="Xaa-Pro aminopeptidase P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0464">Manganese</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="7">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="6">
        <location>
            <begin position="322"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="8">
        <location>
            <begin position="524"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF16188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="587" mass="67957" checksum="492430AE2BF2DF93" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTL8</accession>
    <name>I6ZTL8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA replication licensing factor MCM6</fullName>
            <ecNumber evidence="12">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_050790</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="12"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264508.1">
        <property type="nucleotide sequence ID" value="XM_009266233.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTL8"/>
    <dbReference type="GeneID" id="20521311"/>
    <dbReference type="KEGG" id="ero:EROM_050790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050790"/>
    <dbReference type="HOGENOM" id="CLU_000995_3_2_1"/>
    <dbReference type="OrthoDB" id="1744952at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902969">
        <property type="term" value="P:mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17757">
        <property type="entry name" value="MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000826">
        <property type="entry name" value="Replicative DNA helicase Mcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008049">
        <property type="entry name" value="MCM6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041024">
        <property type="entry name" value="Mcm6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF43">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18263">
        <property type="entry name" value="WHD_MCM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01662">
        <property type="entry name" value="MCMPROTEIN6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="13">
        <location>
            <begin position="302"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="715" mass="81164" checksum="99037CDFFFA33C4A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU53</accession>
    <name>I6ZU53_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264673.1">
        <property type="nucleotide sequence ID" value="XM_009266398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU53"/>
    <dbReference type="GeneID" id="20521479"/>
    <dbReference type="KEGG" id="ero:EROM_060830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060830"/>
    <dbReference type="HOGENOM" id="CLU_1408745_0_0_1"/>
    <dbReference type="OrthoDB" id="2190924at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="193" mass="22763" checksum="8B621A01E6FB6510" modified="2012-10-03" version="1">MDDSIYFRIKKLAEAGVFKNPEIDRLGYGTFQRRQAVESSPSIQKARDLRSRVDAVLKESTHKGIKMVMEVIMMYIKGYMEESSRYKTVDIIRGWKSLGKYIREMVGSLSEEEDIVTFLRLVLFNVKFHYLYLESSLIIKQGRRNESKEGVLAYFLNEYNDLYNIFILSKMRKFHVLRPDDLSDMIKEKINSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUD7</accession>
    <name>I6ZUD7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ubiquitin carboxyl terminal hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264783.1">
        <property type="nucleotide sequence ID" value="XM_009266508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUD7"/>
    <dbReference type="GeneID" id="20521594"/>
    <dbReference type="KEGG" id="ero:EROM_070350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070350"/>
    <dbReference type="HOGENOM" id="CLU_008279_1_0_1"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="1">
        <location>
            <begin position="13"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="297" mass="34238" checksum="522FB68F955585B4" modified="2012-10-03" version="1">MVVQEKKSNLPPKGLKNKGNTCFFNSAMQCILSISELNAFYRDTLLPKEKVISNALKQFISGYENSPQSYDPRNLISVLSTKMKLFNGMQQDSHEFLIQFIDLLYTEFDWNKDMFTSMDEFEEARRKNLIAKTLLSMDRQVLICERCKHSSTTIVINSTILIEPYTSVQEGIDRYYEEVILEKENGWKCDRCGKGMGAIKRMENLIHPEVLTVHISRFHPRGSKDTASVDVNTTLFFNNRKYNLFGVVCHTGTLSGGHYFAEAKRSGTWNVYNDEQVTKGFQTYSGSHPYLAFYTIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUJ4</accession>
    <name>I6ZUJ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264858.1">
        <property type="nucleotide sequence ID" value="XM_009266583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUJ4"/>
    <dbReference type="GeneID" id="20521670"/>
    <dbReference type="KEGG" id="ero:EROM_071100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071100"/>
    <dbReference type="HOGENOM" id="CLU_2134259_0_0_1"/>
    <dbReference type="OrthoDB" id="2189571at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="113" mass="12991" checksum="E7F1339AC3F0B793" modified="2012-10-03" version="1">MIRLGERATFGKIYQIRYKDRMLLKRLCGVILIQTYGMKIEGSITCTSEGDLLEILKSLALKGKDIAILSPSTLIVNREIYKMFRLLNAVGISLFLFILQDDPVWYMDEVMQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV32</accession>
    <name>I6ZV32_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Sar1 GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_081920</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265103.1">
        <property type="nucleotide sequence ID" value="XM_009266828.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV32"/>
    <dbReference type="GeneID" id="20521927"/>
    <dbReference type="KEGG" id="ero:EROM_081920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081920"/>
    <dbReference type="HOGENOM" id="CLU_040729_12_4_1"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="207" mass="23411" checksum="09AFB2D560D63252" modified="2012-10-03" version="1">MGTVISKVNNILYAKLRGIFSGKTERSITMIGLDGAGKTTLLLYLQTGEVHQTVPTLGFNCENVNLGNMKFQVWDIGGQNSFMRFWHQYINDGCGIIYMVDCADPQRFGKSSEELWRILNILNTPRPLLVLANKIDLLKEHERSEVIKSIRNEFNLEKYNGPSQIVPISVLQAGSMTSADDENGREIIDAFKWLNKELEKMPKTEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW48</accession>
    <name>I6ZW48_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-3-methyladenine glycosylase II</fullName>
            <ecNumber evidence="4">3.2.2.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">3-methyladenine DNA glycosidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_051650</name>
        <name evidence="10" type="ordered locus">EROM_110010</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.</text>
            <dbReference type="EC" id="3.2.2.21"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA glycosylase MPG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.21" evidence="4"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264592.1">
        <property type="nucleotide sequence ID" value="XM_009266317.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265483.1">
        <property type="nucleotide sequence ID" value="XM_009267208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW48"/>
    <dbReference type="GeneID" id="20521397"/>
    <dbReference type="GeneID" id="20564601"/>
    <dbReference type="KEGG" id="ero:EROM_051650"/>
    <dbReference type="KEGG" id="ero:EROM_110010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110010"/>
    <dbReference type="HOGENOM" id="CLU_060471_0_2_1"/>
    <dbReference type="OrthoDB" id="5568149at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003905">
        <property type="term" value="F:alkylbase DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00540">
        <property type="entry name" value="AAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.300.10:FF:000001">
        <property type="entry name" value="Putative 3-methyladenine DNA glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.300.10">
        <property type="entry name" value="Methylpurine-DNA glycosylase (MPG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00527">
        <property type="entry name" value="3MGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003180">
        <property type="entry name" value="MPG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036995">
        <property type="entry name" value="MPG_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00567">
        <property type="entry name" value="3mg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10429:SF0">
        <property type="entry name" value="DNA-3-METHYLADENINE GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02245">
        <property type="entry name" value="Pur_DNA_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="22437" checksum="C6731CC1692A2E90" modified="2012-10-03" version="1">MEIPCIELARELLGKILCRRMEGKTMKGMIVETEAYVGREDKACHGYGGRRTERNSAMYMRAGTCYVYKIYGMYECFNISSVEDGAGVLIRAVEPLCGISLMRRHRGGRVKDRDIANGPSKLCIAMGITRKEIDKEWIMSSSKIWIEEGMEVADTSVIVGKRIGIRNCGEWEEKELRFYIKDNEFVSCIRRKRGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW64</accession>
    <name>I6ZW64_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RAB protein geranylgeranyltransferase subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265498.1">
        <property type="nucleotide sequence ID" value="XM_009267223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW64"/>
    <dbReference type="GeneID" id="20564616"/>
    <dbReference type="KEGG" id="ero:EROM_110170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110170"/>
    <dbReference type="HOGENOM" id="CLU_765103_0_0_1"/>
    <dbReference type="OrthoDB" id="2195620at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005092">
        <property type="term" value="F:GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="362" mass="41500" checksum="B20D8C81E1CB3A99" modified="2012-10-03" version="1">MEKFELFDLGFEGTTIRDCLRFRKEKISNAIVFDDNGTYGSTYCGYSSLKNSKYVKDCTLNKGSGESKLLLEAFPAVFRPTDRHMAELEGAGMMGFLRLIKIQKHFYIDSSGKIYKIPYTKTEIADSDILSLREKYLVGSLLKGRLTFENVYKKLSEKTREILTNGIIGEAKDYSERMFRYARNFGNVPFLYPEYGLKEISEMFSRSNSMCGAGYVLDSALVITCRDGKSSDAFSLKQNGGEPIPSEYMYRIDTTYGTIFTKKVIRTEYKELVSYVRVVNTRRPILSKTFFAFAQRESIMKLIGLNSDTECCPEGTYLIYIIKSDGPVVEEDLKFLRIEEKDIVEDISYQTKFSFVFDSLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWF1</accession>
    <name>I6ZWF1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADPH adrenodoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_111200</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265598.1">
        <property type="nucleotide sequence ID" value="XM_009267323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWF1"/>
    <dbReference type="GeneID" id="20564719"/>
    <dbReference type="KEGG" id="ero:EROM_111200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111200"/>
    <dbReference type="HOGENOM" id="CLU_077212_0_0_1"/>
    <dbReference type="OrthoDB" id="333024at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051793">
        <property type="entry name" value="NADH:flavin_oxidoreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42917">
        <property type="entry name" value="2,4-DIENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42917:SF2">
        <property type="entry name" value="2,4-DIENOYL-COA REDUCTASE [(2E)-ENOYL-COA-PRODUCING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="258" mass="29042" checksum="A7E37131501D5D28" modified="2012-10-03" version="1">MKVCIIGGGPAGLYTAASLVPRNIDVTLHEKEPEVGGLYRYSLLPPSRMSPFSKLFKCKNFSLKLNSEVDIERLRGMEKEFDAFVIATGSPGSRRLSIPGREYCINGLDIARSWGGEQPGYSIGNKVLIIGMGDVSMDIVRFLFGWSGAQFKFPKNTLERVKKVEDVTITSRRDAYSSAFTNTGLRKVLEIPGLGFWWSEGKDMSQEERSRSENLKKVYNQGGEDETKDKHKGWWSRRLGLLGGIKEGVRKLRLMFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWG7</accession>
    <name>I6ZWG7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Co/Zn/Cd transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_111400</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265618.1">
        <property type="nucleotide sequence ID" value="XM_009267343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWG7"/>
    <dbReference type="GeneID" id="20564739"/>
    <dbReference type="KEGG" id="ero:EROM_111400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111400"/>
    <dbReference type="HOGENOM" id="CLU_013430_0_1_1"/>
    <dbReference type="OrthoDB" id="9944568at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098771">
        <property type="term" value="P:inorganic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030003">
        <property type="term" value="P:intracellular monoatomic cation homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050681">
        <property type="entry name" value="CDF/SLC30A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562">
        <property type="entry name" value="CATION EFFLUX PROTEIN/ ZINC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11562:SF17">
        <property type="entry name" value="RE54080P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0864">Zinc transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="28"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="226"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN84121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="334" mass="37448" checksum="970AFBF9CB1B98D8" modified="2012-10-03" version="1">MHSRKNSTQCSHVTCSNDADIKKISKVLFIILMFMFLELWGHWKTNSLSLLADSLHLLVDILGFIVSLLSLSWAKKPSSKRMTFGYHRIEIIGSLVSIGLIWAAVGYLAVESFHKYLHPSEIDGGMFFSIAVVGFFVNCVCVYVLHYDEYQHKLKHKNLNIRATYVHVVGDLIQSVGVIIAGMVTYFYPSKAIVDVICTMFFSVLVLVSTGFVLKDGVRILAEGAPRDLDIDGMRADVLEVENVYKIVDLYAWSISMNRTAVSIKILADDLLISDYENILLEVNHIIKTKYLVDIVIIQIDTPNTFYGDKGFVVDGVVIDLKALETPGMMIPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACP6</accession>
    <name>I7ACP6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dynein light chain 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263847.1">
        <property type="nucleotide sequence ID" value="XM_009265572.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACP6"/>
    <dbReference type="GeneID" id="20520631"/>
    <dbReference type="KEGG" id="ero:EROM_010050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010050"/>
    <dbReference type="HOGENOM" id="CLU_1948794_0_0_1"/>
    <dbReference type="OrthoDB" id="2190967at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030286">
        <property type="term" value="C:dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21450">
        <property type="entry name" value="DLC-like_DYNLL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.740.10">
        <property type="entry name" value="Protein Inhibitor Of Neuronal Nitric Oxide Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037177">
        <property type="entry name" value="DLC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001372">
        <property type="entry name" value="Dynein_light_chain_typ-1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01221">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01375">
        <property type="entry name" value="Dynein_light"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54648">
        <property type="entry name" value="DLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="127" mass="13959" checksum="74498009350DF147" modified="2012-10-03" version="1">MKSEDTSDSSSGESKKSTAPGSQRSQEAKSIVNGPKVIEPIFEQREPLDDQIKSQIILSLAMFTDETPPTRICEALKSMLDSKFGKGWCVFAGAHFAGSCTFEEKYFAQISFGTYSITVFRIFIPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD06</accession>
    <name>I7AD06_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ran GTPase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263977.1">
        <property type="nucleotide sequence ID" value="XM_009265702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD06"/>
    <dbReference type="GeneID" id="20520763"/>
    <dbReference type="KEGG" id="ero:EROM_020050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020050"/>
    <dbReference type="HOGENOM" id="CLU_104726_0_0_1"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00835">
        <property type="entry name" value="RanBD_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="87"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="219" mass="25422" checksum="9E092476289BC29B" modified="2012-10-03" version="1">MAEVEKKGEHEKIETIPKKQKEEVPGGMEKEGLADSVKKEVETKKAHQSPFLTNGVPRKDETKGGEEKDKIDVTEVVEKQRKHLEENQSDEVLFKARCKLYYFSEETKALEERAEGTMIIGMHSKSNLVKITVFRDQIGRLGCNHFINPRFKAQPHGKVSNGWMWMSTEDTVEVDAQRKKIQLFVVKFYSEEDYRRFGEEYDLGRSHNEKSLKTKVNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD35</accession>
    <name>I7AD35_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zn-ribbon-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_020400</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anamorsin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264012.1">
        <property type="nucleotide sequence ID" value="XM_009265737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD35"/>
    <dbReference type="GeneID" id="20520798"/>
    <dbReference type="KEGG" id="ero:EROM_020400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020400"/>
    <dbReference type="HOGENOM" id="CLU_121509_2_0_1"/>
    <dbReference type="OrthoDB" id="311633at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anamorsin C-terminal" evidence="10">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="112" mass="12582" checksum="F402E48E90147E3A" modified="2012-10-03" version="1">MEDKEELRKLLRSIIKKNTDPRARTQSEYLTDEDKVVKKHERGSVKTRACKDCTCGLKEKQEIKIRSACGNCHKGDAFRCSGCPSLGLPPYEAGEVVSFSADLNDGFQDQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADN7</accession>
    <name>I7ADN7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264177.1">
        <property type="nucleotide sequence ID" value="XM_009265902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADN7"/>
    <dbReference type="GeneID" id="20520970"/>
    <dbReference type="KEGG" id="ero:EROM_030590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030590"/>
    <dbReference type="HOGENOM" id="CLU_2049681_0_0_1"/>
    <dbReference type="OrthoDB" id="2189995at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="13657" checksum="87D6320204381AE2" modified="2012-10-03" version="1">MLYQEKKEDKATRSLIEDASLFLWFVNAVNSGGYCKVVKSEEDISLTPKKKFHQKIKFKAYSMSADNIMYEKRDEIAFEEDRNGFICAHCDARYVYKRCLVNHLIKSHHINSNTFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADP7</accession>
    <name>I7ADP7_ENCRO</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264192.1">
        <property type="nucleotide sequence ID" value="XM_009265917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADP7"/>
    <dbReference type="GeneID" id="20520985"/>
    <dbReference type="KEGG" id="ero:EROM_030740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030740"/>
    <dbReference type="HOGENOM" id="CLU_167709_0_0_1"/>
    <dbReference type="OrthoDB" id="2190000at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="118" mass="12872" checksum="75C5E3BE65D62CAF" modified="2012-10-03" version="1">MSLRDYTLAIFEATGNSFTIGCSMALASNMFRREGEHSYSRQPLRSGGELAKHTMIYSFLYYGLSGVGASRWIRLLGPSFVASLVCGMRNGRGFAIRSGIDGMMSSLVQEVISKIKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADU0</accession>
    <name>I7ADU0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264252.1">
        <property type="nucleotide sequence ID" value="XM_009265977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADU0"/>
    <dbReference type="GeneID" id="20521047"/>
    <dbReference type="KEGG" id="ero:EROM_031330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031330"/>
    <dbReference type="HOGENOM" id="CLU_921426_0_0_1"/>
    <dbReference type="OrthoDB" id="2190809at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="302" mass="34726" checksum="C8FA5FF0E9E59975" modified="2012-10-03" version="1">MKKSPCLGEILSIMGALQLYLVSGYLESHFMMIFCIENLLSTLVVLMASAPFAIKSTAFKEVFQAQSEYLPFGLFAFGEYSSWYRGAGMISLKTAMATRGLCYPLSVVLMFMLSRVARENMLRNFGLILIILMNMYKSVKYSDEGFFDVFFGTIYLVLYVIFSVSSDFITVCMTNRNGLPKFLFASSFYIWICSLVALIDSGIGHNDIYTFIERNIYRSILLVIISSLSRILSAYYIQRFGIVAYSTTKISNYIYFIILRSMMGLEWSWRFEDTLFHLSIYFLILFFIDADESYERSLGFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADX3</accession>
    <name>I7ADX3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16">DNA topoisomerase 2</fullName>
            <ecNumber evidence="5 16">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">EROM_040310</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 16">Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 16">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5 16"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264297.1">
        <property type="nucleotide sequence ID" value="XM_009266022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADX3"/>
    <dbReference type="GeneID" id="20521095"/>
    <dbReference type="KEGG" id="ero:EROM_040310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040310"/>
    <dbReference type="HOGENOM" id="CLU_001935_1_0_1"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16930">
        <property type="entry name" value="HATPase_TopII-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03481">
        <property type="entry name" value="TopoIIA_Trans_ScTopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03365">
        <property type="entry name" value="TOPRIM_TopoIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.30:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000008">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000004">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001241">
        <property type="entry name" value="Topo_IIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013506">
        <property type="entry name" value="Topo_IIA_bsu_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018522">
        <property type="entry name" value="TopoIIA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034157">
        <property type="entry name" value="TOPRIM_TopoII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00204">
        <property type="entry name" value="DNA_gyraseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00418">
        <property type="entry name" value="TPI2FAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00433">
        <property type="entry name" value="TOP2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00177">
        <property type="entry name" value="TOPOISOMERASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 15 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13 15 16" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 16" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Toprim" evidence="18">
        <location>
            <begin position="422"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="19">
        <location>
            <begin position="665"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1087"/>
            <end position="1144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1091"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1120"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="15">
        <location>
            <position position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU362094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AFN82800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1144" mass="130715" checksum="E34363B9996D823F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE57</accession>
    <name>I7AE57_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_041510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264412.1">
        <property type="nucleotide sequence ID" value="XM_009266137.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE57"/>
    <dbReference type="GeneID" id="20521214"/>
    <dbReference type="KEGG" id="ero:EROM_041510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041510"/>
    <dbReference type="HOGENOM" id="CLU_103055_0_0_1"/>
    <dbReference type="OrthoDB" id="2189364at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="5">
        <location>
            <begin position="20"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="216" mass="23913" checksum="6B7048F2CB75F120" modified="2012-10-03" version="1">MSDGEGYEEVLKNNISESSMKCASCGWSGKMKIVDLSDVTENVICAFVCKKCGDKSVNFLEKTCDPRGSVRIECFFDDKEDLSREVNLSQLASVEIISDDLSFKLSSTYPSIQNVESFLIQGKDQIKNLCGKEDITSGAGGGNVLADGDISKETCEKKLDDFQKLMANPKFKLIIEDDFGLSRVAPAGKNVLELRDADVKELNDEKVKHIFRKKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEB3</accession>
    <name>I7AEB3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_050570</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264487.1">
        <property type="nucleotide sequence ID" value="XM_009266212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEB3"/>
    <dbReference type="GeneID" id="20521289"/>
    <dbReference type="KEGG" id="ero:EROM_050570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050570"/>
    <dbReference type="HOGENOM" id="CLU_101433_0_0_1"/>
    <dbReference type="OrthoDB" id="18915at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22749">
        <property type="entry name" value="Otubain_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.60">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.20">
        <property type="entry name" value="Peptidase C65 Otubain, subdomain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019400">
        <property type="entry name" value="Peptidase_C65_otubain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042468">
        <property type="entry name" value="Peptidase_C65_otubain_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042467">
        <property type="entry name" value="Peptidase_C65_otubain_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931:SF15">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTUBAIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12931">
        <property type="entry name" value="UBIQUITIN THIOLESTERASE PROTEIN OTUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10275">
        <property type="entry name" value="Peptidase_C65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="8">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="27396" checksum="F95E9F933DFF32D6" modified="2012-10-03" version="1">MKSYTEFRECIRDGNCLYISYAVVLADLVTSEGEPLLKTLTSAFAKTNSMLHLYNIDELGYKGFYDSFADILADMVNGTKKIEDVPLYSLYDCVAYLRLVVSTEIKSNPEKYQPYIPEMDVDKYCTRFVDPFYQQAGCVEICALSNSIPIRIHIVDITKENEDVYGDHTQHVSIFYTHGHFEPIYQDRREDSRPYFPDKKLDSLRANGGEACNKNQEGCQHLGQKKSKGGEAEEYTEHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEK9</accession>
    <name>I7AEK9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polar tube protein Ptp2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264607.1">
        <property type="nucleotide sequence ID" value="XM_009266332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEK9"/>
    <dbReference type="GeneID" id="20521412"/>
    <dbReference type="KEGG" id="ero:EROM_060150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060150"/>
    <dbReference type="HOGENOM" id="CLU_1004828_0_0_1"/>
    <dbReference type="OrthoDB" id="2190601at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031507">
        <property type="entry name" value="PTP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17022">
        <property type="entry name" value="PTP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="274" mass="29566" checksum="666017CFCC5A9836" modified="2012-10-03" version="1">MLLLFTVVTLVSTAQVPQTPASPQTAPTQFLPGVQQKIGGVDNRCANKQVEGVQIFQGNMAECLKRNSEAANAMFQRAKQRALEIYNKEVSKGPTPKDSGQCIERAVQGTDRCILAKIIDKAVNMLKYRISKVGNATALFRGNKLISLILNVDYGLKPFFTVVKKKTKRVFPSGDELNFNGIGQLIGVKGTFPQDNNDECKPCDSPKKTVETVAEECNLGCQLKGTPGLISKAIQKKDTKESSKDGEKGSAQNGEGTTDDEDGQQSPADGNGPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEW4</accession>
    <name>I7AEW4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S ribosomal protein L19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_061030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264692.1">
        <property type="nucleotide sequence ID" value="XM_009266417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEW4"/>
    <dbReference type="GeneID" id="20521499"/>
    <dbReference type="KEGG" id="ero:EROM_061030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061030"/>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_1"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="4">
        <location>
            <begin position="3"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="20405" checksum="2ECDC94BD97B77B9" modified="2012-10-03" version="1">MSNEKRIRRLASEILNCGKNKLWLDNNEIERLNSATSREQIRQLIKDNVIIMKQDKHTSRGRFRERLRAKRKGRHMGPGKRFGTTNARMPKKELWMKKIRAMRKMLKEMRASGEITKEDFRVFYKQAKGHLFKHRFHMKDCIVKRKIEEQRARELAEQAEALNLSNRAVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF02</accession>
    <name>I7AF02_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_061540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264742.1">
        <property type="nucleotide sequence ID" value="XM_009266467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF02"/>
    <dbReference type="GeneID" id="20521551"/>
    <dbReference type="KEGG" id="ero:EROM_061540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061540"/>
    <dbReference type="HOGENOM" id="CLU_038526_1_0_1"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="97"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="318" mass="34967" checksum="413DADCA8816148B" modified="2012-10-03" version="1">MAQYSAKISDIPNPPSDTVSEIAFSQMHSLMAASSWDGTIRTYDLENLYSSNTSVVNLNKPLLTCCFSKETPSLAFAGAADGSLQVVDLQTSQVSSFQAHNAGVKSVRCFSNMLATGSWDKTVKFWDTRSSKLVFSLDLPGKVYAMDLEKELLAISLSGNEVVTYNLNDINQKKTHASKLNWMIRSIACAQDNETFALGGIEGKAEIFNINSPVKKMIFRCHRVDNKVYAVNSVSFLPTNHNILVTAGGDGTIVFFDAQARMKIFTQTESQPITCGRFNTNGNYYVYATGNDWSTGYVTTYRPTQLKAIQVSTTGVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF57</accession>
    <name>I7AF57_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proline hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_070640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264812.1">
        <property type="nucleotide sequence ID" value="XM_009266537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF57"/>
    <dbReference type="GeneID" id="20521623"/>
    <dbReference type="KEGG" id="ero:EROM_070640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070640"/>
    <dbReference type="HOGENOM" id="CLU_816288_0_0_1"/>
    <dbReference type="OrthoDB" id="430522at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051842">
        <property type="entry name" value="uS12_prolyl_hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12117:SF0">
        <property type="entry name" value="PROLYL 3-HYDROXYLASE OGFOD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="76"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="349" mass="40596" checksum="114A339A28BE8C84" modified="2012-10-03" version="1">MKRYERPFLHFVKDDFLSPSDVEAIRDVHGRCAFQELHTDLFRFLQTGELKEREDLAFFKSSLMKVFEEIGDERGGWIDLFGSYYRKGDYLLCHDDKTGSRRFAFSYYLEDYDSGELILYENDCMTVSKRVEVKANRLVVFEVSDISFHEVGYCEKDGRRAFTGWLNFAGIKHDGGSRSMDVLGITGYETFPLEVDFRESSFVFFPGVEYDFECVSRSVEGPFYLRRVERIEISNPLVPTMEGWKLKDGGFYHFRVGDYILLNDRCNTVEGNVCDLFFIEGEEAACCDNSEVFKDPINPVKYLDEEGRFVCGLPITKGMFAIKRDGFSLFVERCNKEFFLAHFIYVQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF61</accession>
    <name>I7AF61_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_070690</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Water channel required to facilitate the transport of water across membranes. Involved in osmotolerance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264817.1">
        <property type="nucleotide sequence ID" value="XM_009266542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF61"/>
    <dbReference type="GeneID" id="20521628"/>
    <dbReference type="KEGG" id="ero:EROM_070690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070690"/>
    <dbReference type="HOGENOM" id="CLU_020019_3_4_1"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="251" mass="26915" checksum="3AD586212D37770F" modified="2012-10-03" version="1">MARETLKTLQSMFGEMVASFVFGFAVYSAVLGSTVSQQSAARVIVGLTVGFSAIGIIYSFGDVTIAHFNPAITLTAILTGKIGIFHGLGYILAQYVGFMLAVCALIPCSPIGYKETLNIIRPAPSSFGGDNLNVFFTEFFLTAIFVHIVFAVAVNPYKPKVDTDGKFVDPDEKEPVDRRITAPLSIGLTLGFLAFLGLASSGGAFNPGLTFAPVIMSNTWTHFWLYFGGQYLGGFVGGLLQVFVLYKLSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFJ9</accession>
    <name>I7AFJ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 4E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_080870</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265002.1">
        <property type="nucleotide sequence ID" value="XM_009266727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFJ9"/>
    <dbReference type="GeneID" id="20521822"/>
    <dbReference type="KEGG" id="ero:EROM_080870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080870"/>
    <dbReference type="HOGENOM" id="CLU_043552_2_1_1"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF8">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="173" mass="19586" checksum="30288FCAFF587435" modified="2012-10-03" version="1">MNLASQWVLWQNNNDESNAKSWGDDLVAVGEVSTIPEFLYLCDEISNAGIGKLCTMNLFKKGVKPMWEDEANIDGGRIIMDVPVVGRDNIGELWKKTMAFCISNVVDNVCGCVLSEKQSFYKLAIWFGKDYNQDTIKEMWQETLESGSLSVYSFLHKKSLDSNKGKKKWGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFN3</accession>
    <name>I7AFN3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase I subunit RPA12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_081340</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265047.1">
        <property type="nucleotide sequence ID" value="XM_009266772.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFN3"/>
    <dbReference type="GeneID" id="20521869"/>
    <dbReference type="KEGG" id="ero:EROM_081340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081340"/>
    <dbReference type="HOGENOM" id="CLU_093932_1_2_1"/>
    <dbReference type="OrthoDB" id="10056816at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10507">
        <property type="entry name" value="Zn-ribbon_RPA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034004">
        <property type="entry name" value="Zn_ribbon_RPA12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
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        <location>
            <begin position="57"/>
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        <location>
            <position position="3"/>
        </location>
        <ligand>
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        <location>
            <position position="5"/>
        </location>
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        </ligand>
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        <location>
            <position position="20"/>
        </location>
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="23"/>
        </location>
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        </ligand>
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        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        <location>
            <position position="64"/>
        </location>
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        <location>
            <position position="89"/>
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            <name>Zn(2+)</name>
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            <position position="91"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00018784"/>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83550.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="99" mass="11114" checksum="03F90995A8C675C7" modified="2012-10-03" version="1">MFCICGTLIYVKSNSTKVECLRCKKENSVDMIRPIYTEVEIKGDSCIEAIDVSGAKIKHRCPACGAEEMMYNTVQVRSTDEGQTVFYSCECGYKFTVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFS7</accession>
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        <recommendedName>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
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            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
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    </comment>
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    </dbReference>
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    </evidence>
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        <source>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="259" mass="30031" checksum="03CBF85A9FB5780B" modified="2012-10-03" version="1">MDKDELSVDGDEVRDGYLDLLQKMRCLKEDLFEDKCRLEELINTSNKLFKKIKTSSELKLDAKMTALSTKIACKRMEKDIELDDITSSTFVELFRKNLLNDFYRYAMRCNAGIRFLGCFSLGNHPEASNRKKLPVQRSKQHLPETRIPALITREKASTEEFDILMHIKEVVLERGRVEYFRLVINPQSFSKTIENIFYLSLSLRSGLIYIEWDDDEILYVTSKNSGRKGDLGHLAIEMTYDEYLAIVEKAGISETLIEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
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    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
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    <dbReference type="OrthoDB" id="10017054at2759"/>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="11"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Calponin-homology (CH)" evidence="3">
        <location>
            <begin position="126"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="565" mass="64886" checksum="1FA22AA54EF3F0AB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG03</accession>
    <name>I7AG03_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RING zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_090330</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003526">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG03"/>
    <dbReference type="GeneID" id="20521971"/>
    <dbReference type="KEGG" id="ero:EROM_090330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090330"/>
    <dbReference type="HOGENOM" id="CLU_1160858_0_0_1"/>
    <dbReference type="OrthoDB" id="5588846at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014891">
        <property type="entry name" value="DWNN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033489">
        <property type="entry name" value="RBBP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025829">
        <property type="entry name" value="Zn_knuckle_CX2CX3GHX4C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439:SF0">
        <property type="entry name" value="CELL DIVISION CYCLE AND APOPTOSIS REGULATOR PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15439">
        <property type="entry name" value="RETINOBLASTOMA-BINDING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08783">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13696">
        <property type="entry name" value="zf-CCHC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01180">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51282">
        <property type="entry name" value="DWNN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DWNN" evidence="9">
        <location>
            <begin position="5"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="202"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="260" mass="30279" checksum="3C7B673DE158BEED" modified="2012-10-03" version="1">MTSVINYRFRSNKNFSRIQFQGTGLPLWELKYEIINQRKMISKDFDLLFFDGETNEEINDEYQLISMNSHVIVNRIPLWMSKGGYNVKEKRLESQGHHNTTQKYNKAVPESYVCFRCGQKGHYIQNCHTNQDKAFDILRIRKPSGIPKDFLVPVSGENVPSNAAMLVTPEGGYVKAQPQVQEWKKYKQRSKMTVEIPDRLKCPSCHCLLVNPIKTSCGHTLCDQCVRMDRKCYVCSKVIENFTQDINMKIEVERAIEQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG31</accession>
    <name>I7AG31_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RuvB-like helicase</fullName>
            <ecNumber evidence="10">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_090780</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">DNA helicase participates in several chromatin remodeling complexes, including the SWR1 and the INO80 complexes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="10"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265192.1">
        <property type="nucleotide sequence ID" value="XM_009266917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG31"/>
    <dbReference type="GeneID" id="20564302"/>
    <dbReference type="KEGG" id="ero:EROM_090780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090780"/>
    <dbReference type="HOGENOM" id="CLU_028311_1_1_1"/>
    <dbReference type="OrthoDB" id="10060499at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.360:FF:000001">
        <property type="entry name" value="RuvB-like helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.360">
        <property type="entry name" value="RuvB-like helicase, domain II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027238">
        <property type="entry name" value="RuvB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041048">
        <property type="entry name" value="RuvB-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042487">
        <property type="entry name" value="RuvBL1/2_DNA/RNA_bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010339">
        <property type="entry name" value="TIP49_P-loop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11093">
        <property type="entry name" value="RUVB-RELATED REPTIN AND PONTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06068">
        <property type="entry name" value="TIP49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17856">
        <property type="entry name" value="TIP49_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 10 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="54"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="426" mass="47469" checksum="33AFF0E2089487FA" modified="2012-10-03" version="1">MVKSSNIAIHSHVKSLGLDIFGRPIEKPNAVIGQEDAREAAGLIVEMVKTKRMSGRAVLISGPAGCGKTALAVGISEELGPGTPFTSMSGSEVYSNEVKKTEVLEEALRRSILVRMRELKDIYEGEIVELRILDEENPLSSYSKKVKEIFITLKTSKESKKLKLASSLYEQIDKQRIVNGDVVYIEVNSGVIKKLGRSEAHMNDFDLEADTYVPIPKGEVLKRKEVIQSVTLHDLDMANAKPTGQDMLSLVFRILSPKKTEITERLRNDVNRMVNEYLEKGNAEIVPGILFIDEVHLLDVECFTFLHKIIESPLSPTVIFASNRGMAPIKGSNGLLGPFGITKDLLDRIMIITVKKNTDESNMKIIEMRLKEEELQMDGDALTFFVNLSISKGLRYCISLIPLLKTYGGCVTIQNVEEVNELFHDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG35</accession>
    <name>I7AG35_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ordered locus">EROM_090840</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="24" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_009266922.1"/>
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    <dbReference type="KEGG" id="ero:EROM_090840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090840"/>
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    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
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        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="term" value="C:trans-Golgi network"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="term" value="P:endocytosis"/>
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        <property type="term" value="P:phospholipid translocation"/>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
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        <property type="entry name" value="ATPase_P-type_domA"/>
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        <property type="entry name" value="HAD_sf"/>
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        <property type="entry name" value="P-type_ATPase_IV"/>
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        <property type="entry name" value="P_typ_ATPase"/>
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        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
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        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cation_ATPase"/>
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        <property type="entry name" value="E1-E2_ATPase"/>
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        <property type="entry name" value="PhoLip_ATPase_C"/>
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        <property type="entry name" value="PhoLip_ATPase_N"/>
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    </dbReference>
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        <property type="entry name" value="CATATPASE"/>
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        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="p-type_atpase"/>
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    </dbReference>
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        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
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        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
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        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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        <property type="entry name" value="ATPASE_E1_E2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 17 19" id="KW-0547">Nucleotide-binding</keyword>
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    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="63"/>
            <end position="84"/>
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    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="373"/>
            <end position="393"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="405"/>
            <end position="424"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="901"/>
            <end position="921"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="927"/>
            <end position="953"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="974"/>
            <end position="994"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1000"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1027"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1058"/>
            <end position="1077"/>
        </location>
    </feature>
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        <location>
            <begin position="13"/>
            <end position="59"/>
        </location>
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            <begin position="109"/>
            <end position="162"/>
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            <begin position="862"/>
            <end position="1064"/>
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            <begin position="259"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="301"/>
            <end position="317"/>
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    </feature>
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            <position position="464"/>
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        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="464"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <position position="465"/>
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        <ligand>
            <name>ATP</name>
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            <position position="466"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="554"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="596"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="618"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
    </feature>
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        <location>
            <position position="647"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="727"/>
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        <ligand>
            <name>ATP</name>
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            <position position="728"/>
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            <name>ATP</name>
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            <position position="729"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="809"/>
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        <ligand>
            <name>ATP</name>
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            <position position="815"/>
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            <name>ATP</name>
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            <name>ATP</name>
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            <dbReference type="ARBA" id="ARBA00004337"/>
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            <dbReference type="ARBA" id="ARBA00008109"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00049128"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
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    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="AFN83700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1097" mass="124705" checksum="C36F0B4E334B2E4F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG42</accession>
    <name>I7AG42_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">GPI mannosyltransferase 2</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_090940</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Endoplasmic reticulum membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the PIGV family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266932.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20564318"/>
    <dbReference type="KEGG" id="ero:EROM_090940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090940"/>
    <dbReference type="HOGENOM" id="CLU_029048_3_1_1"/>
    <dbReference type="OrthoDB" id="10252502at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031501">
        <property type="term" value="C:mannosyltransferase complex"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:alpha-1,6-mannosyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004376">
        <property type="term" value="F:GPI mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007315">
        <property type="entry name" value="PIG-V/Gpi18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12468:SF2">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04188">
        <property type="entry name" value="Mannosyl_trans2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 12" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="255"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="370" mass="43000" checksum="2A63848BFC3492CB" modified="2012-10-03" version="1">MILGNNTQKILCLAVASKVFYVGISYFAYLLLPRFDKSTELISTNSCLKFLLSWDAIHFLEIMEKGYGKAHVVAFFPLLPYMSRVVARLLSLEPYTTGVLVSCTASVLSSVLLYRISQRRYGNKIAIMSCILFIFNPAAIVYTAMYSESLFMLLFLLGIYFLENNSIVHGTLFLSLCGLCRSNAILFAPFIIYPLGKNALLRMIVLLLPLGIFQYYSLLMINKATNTYRIFIPYFYIQKTLWDQGFLRFFTLKNIPNVLVGLPFILISLYILREYWNKRSTVNPKMYMDTQRFNTDMCAIVLLVQTLITIFLIHWNMYFRFISFNPLIYWMLAEKYYTIEKGFGKRLLFRFYFGFGVAYAVLFGCFYPPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGG2</accession>
    <name>I7AGG2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ADP,ATP carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_100390</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ADP/ATP translocase tlc family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265352.1">
        <property type="nucleotide sequence ID" value="XM_009267077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGG2"/>
    <dbReference type="GeneID" id="20564469"/>
    <dbReference type="KEGG" id="ero:EROM_100390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100390"/>
    <dbReference type="HOGENOM" id="CLU_023964_1_0_1"/>
    <dbReference type="OrthoDB" id="2190844at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005471">
        <property type="term" value="F:ATP:ADP antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004667">
        <property type="entry name" value="ADP_ATP_car_bac_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31187:SF1">
        <property type="entry name" value="ADP,ATP CARRIER PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03219">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="543" mass="61776" checksum="F062BCF400074280" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGM2</accession>
    <name>I7AGM2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">EROM_101100</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265422.1">
        <property type="nucleotide sequence ID" value="XM_009267147.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20564540"/>
    <dbReference type="KEGG" id="ero:EROM_101100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101100"/>
    <dbReference type="HOGENOM" id="CLU_000556_1_1_1"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="13 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="11 15" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="16">
        <location>
            <begin position="199"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase epsilon ,catalytic subunit A thumb" evidence="18">
        <location>
            <begin position="874"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="17">
        <location>
            <begin position="1454"/>
            <end position="1584"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase-epsilon zinc finger" evidence="19">
        <location>
            <begin position="1720"/>
            <end position="1756"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF22634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF22912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="AFN83925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1779" mass="207930" checksum="61721C6E0B79329E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH66</accession>
    <name>I7AH66_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Folate transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265627.1">
        <property type="nucleotide sequence ID" value="XM_009267352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH66"/>
    <dbReference type="GeneID" id="20564748"/>
    <dbReference type="KEGG" id="ero:EROM_111490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111490"/>
    <dbReference type="HOGENOM" id="CLU_051363_0_0_1"/>
    <dbReference type="OrthoDB" id="2191737at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090482">
        <property type="term" value="F:vitamin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002666">
        <property type="entry name" value="Folate_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686">
        <property type="entry name" value="FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686:SF18">
        <property type="entry name" value="IP11787P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="213"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="321"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="48983" checksum="9478B000991FFE50" modified="2012-10-03" version="1">MSWTVRLYQYFGLKSFTPFLSFMVPYLVEQKGFRNGELHNNFSPLFFTSSIIVSLTSFLIIELLGNRMSLFVDTFIEVVVYLIFMFMPFRNSFLTATVYVLHGATTSFGVVMKSILNSTAERKEDRKVILSTAINIKTSVSVVSSWIGQDIIMCGGCHRVNILISLLGLFLALLTTFLLPATVPNKEEERLIDYMWNLSEAWKKIKSTYKPSVVLASLLSSSASILYICLSFYSAGIFLEKKKNGMESSIKINRMLFLIAKPVRFVSYAVIKIFSNFDSSVSYHSNPSKPVIIHGYIEGCSKILSSFAGLQISKVLSESRCFPLAFLTSMATMAFIYCLGASNSMSYSYLFYILASLSSSLCKTISNIGLQSVCEESGYKFVLGFNLFVSSIIHISITHFSRAYSLSVKSKLMLYFYTNAFFIMVAFGIYVLGQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALD7</accession>
    <name>I7ALD7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263981.1">
        <property type="nucleotide sequence ID" value="XM_009265706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALD7"/>
    <dbReference type="GeneID" id="20520767"/>
    <dbReference type="KEGG" id="ero:EROM_020090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020090"/>
    <dbReference type="HOGENOM" id="CLU_2015251_0_0_1"/>
    <dbReference type="OrthoDB" id="10587078at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="123" mass="13805" checksum="4EF7546C7A61B77B" modified="2012-10-03" version="1">MRCLPYIILFTFSKMHGISLFHQNVLRKEDVKNMASRLNSTIDNKRSNLFGDRKKFLSFLKEEKESNDSQESGKNEESGKNEESGEGEESGDNTVKRLSKASPYLKLGNNLGKTVFGRGGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALG7</accession>
    <name>I7ALG7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">T complex protein 1 delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_020440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264016.1">
        <property type="nucleotide sequence ID" value="XM_009265741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALG7"/>
    <dbReference type="GeneID" id="20520802"/>
    <dbReference type="KEGG" id="ero:EROM_020440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020440"/>
    <dbReference type="HOGENOM" id="CLU_008891_2_2_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="121" mass="13145" checksum="B7E522221BEE11CA" modified="2012-10-03" version="1">MAERLNQVRASIFQASQSLLQTLSTSLGPRGLDKMIVRDKKTVVTNDGATILKYLNQHPIHGILSNMSAAQDEECGDGTTSVVVLAGCLLESISNLLERNVHPSVICDNLEIAKRIGHQVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALJ3</accession>
    <name>I7ALJ3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_020690</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264041.1">
        <property type="nucleotide sequence ID" value="XM_009265766.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALJ3"/>
    <dbReference type="GeneID" id="20520829"/>
    <dbReference type="KEGG" id="ero:EROM_020690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020690"/>
    <dbReference type="HOGENOM" id="CLU_112958_2_2_1"/>
    <dbReference type="OrthoDB" id="1727351at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="120" mass="14058" checksum="8E90F11045CBD90B" modified="2012-10-03" version="1">MGSVKNKIVKRAARTIAEKYFQRLDNTFDHNLLVVQDVAVVESKKLKNEIAGYLTSLYKKILKGTYNRVYIKSHEEERERKENVIPKESMLDVDCVEVDKVTMDMIKRYGYEGNFKIYGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALQ0</accession>
    <name>I7ALQ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_021240</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase G subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264096.1">
        <property type="nucleotide sequence ID" value="XM_009265821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALQ0"/>
    <dbReference type="GeneID" id="20520886"/>
    <dbReference type="KEGG" id="ero:EROM_021240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021240"/>
    <dbReference type="HOGENOM" id="CLU_177151_0_0_1"/>
    <dbReference type="OrthoDB" id="2193583at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005124">
        <property type="entry name" value="V-ATPase_G"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03179">
        <property type="entry name" value="V-ATPase_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="6"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="105" mass="12406" checksum="2A2E79FFF7EB92DF" modified="2012-10-03" version="1">MSVESIKRLVQCENEAKERLEIARREFEEMRVQARNDAKLMVDGLKKENESKLLLLEKEVEEYIKIVDEKLKSELEMKIKELRNIRNRKDVVLALVKHVSGVNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALU7</accession>
    <name>I7ALU7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GDP-mannose transporter</fullName>
            <shortName evidence="5">GMT</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030050</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homooligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Golgi apparatus membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TPT transporter family. SLC35D subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264121.1">
        <property type="nucleotide sequence ID" value="XM_009265846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALU7"/>
    <dbReference type="GeneID" id="20520912"/>
    <dbReference type="KEGG" id="ero:EROM_030050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030050"/>
    <dbReference type="HOGENOM" id="CLU_025360_1_0_1"/>
    <dbReference type="OrthoDB" id="417037at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="292" mass="32647" checksum="DFB476F45654A719" modified="2012-10-03" version="1">MKKPIEELMASLTYLFSSIITTLSNKYILSNLGFKMQYLLVAAQSFTIVMGLLVLRGMRLIEFKLTNFRKWMIPSLLLTVMIFSGSKSLYYLPISLYTLFKNSSIVVVALLEQYLFEKRIGALSYLSFILMIISSYTGNSSDVILSIGYAWILINILSTTAYVLSLKVVVDMNSKAKTESVYYSNLISLPILTFLSIFFDEKDASVFGFKVFIWILISSLCAFFTSFSTAWTLNVLSSTALSMIGALNKSLGSFAGIFVLGEIINAQKILSLLLGSLASAIYSYDISFRKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM26</accession>
    <name>I7AM26_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_031020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264221.1">
        <property type="nucleotide sequence ID" value="XM_009265946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM26"/>
    <dbReference type="GeneID" id="20521015"/>
    <dbReference type="KEGG" id="ero:EROM_031020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031020"/>
    <dbReference type="HOGENOM" id="CLU_2015252_0_0_1"/>
    <dbReference type="OrthoDB" id="2190174at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="123" mass="13820" checksum="C6D6F088061646C5" modified="2012-10-03" version="1">MASRTGLPLKILSTSLKKLRYISGAMFCGRKEVSHVEIAGTCIYKGKTSSRILDFCGEVPVEMGDQEIDLGSSYLLVCKIYIRNGVIGLYCKSARKMGIFEEMFFWAEVVSLGRTLQCIRNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM54</accession>
    <name>I7AM54_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Mot1 helicase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_031420</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264261.1">
        <property type="nucleotide sequence ID" value="XM_009265986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM54"/>
    <dbReference type="GeneID" id="20521056"/>
    <dbReference type="KEGG" id="ero:EROM_031420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031420"/>
    <dbReference type="HOGENOM" id="CLU_005404_0_0_1"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003398">
        <property type="entry name" value="Mot1 helicase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000090">
        <property type="entry name" value="Similarity to HELICASE MOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044972">
        <property type="entry name" value="Mot1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36498:SF1">
        <property type="entry name" value="TATA-BINDING PROTEIN-ASSOCIATED FACTOR 172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="809"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="1087"/>
            <end position="1246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1256" mass="144519" checksum="8617C054DC6F2755" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM66</accession>
    <name>I7AM66_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_040080</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264276.1">
        <property type="nucleotide sequence ID" value="XM_009266001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM66"/>
    <dbReference type="GeneID" id="20521072"/>
    <dbReference type="KEGG" id="ero:EROM_040080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040080"/>
    <dbReference type="HOGENOM" id="CLU_1199812_0_0_1"/>
    <dbReference type="OrthoDB" id="2190869at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="227" mass="25622" checksum="1639196FCC585137" modified="2012-10-03" version="1">MPSKALSQNFEDLQPVVIAGCVIQSSYALVENGIDATLSDLILPGFMNFFGPGLCANIVYKQAELSRMSLISYFIGIIIFSIFSQMRILWISTAIFPVIGRGFMLITLKNNNKPFHLVIAWLMALEMSGVLMQKLLFNKRMKVSGDKAGEIFILILGLGLGRLYRLPDYTMMLVVFLVALGPFVQYMFSLPLFSQKKQKKERGRYPRIKLPKRKAAQKAQDTLKQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMP1</accession>
    <name>I7AMP1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264496.1">
        <property type="nucleotide sequence ID" value="XM_009266221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMP1"/>
    <dbReference type="GeneID" id="20521299"/>
    <dbReference type="KEGG" id="ero:EROM_050670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050670"/>
    <dbReference type="HOGENOM" id="CLU_097614_0_0_1"/>
    <dbReference type="OrthoDB" id="2196114at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051190">
        <property type="entry name" value="Baculoviral_IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46771:SF5">
        <property type="entry name" value="DETERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="231" mass="26766" checksum="968242116C67C52D" modified="2012-10-03" version="1">MFSFKERMETFAKWPEGYGVATPERLSVAGFVCLSTEEGNLTVECVYCHKTLECWERTDLPVREHYLHMSKCPLFNVNKIESRVNMFNGWDSKEAKALARIGFVKYNLGDADFIFCYKCGSIDKGHSCRRKRGSVYNLERRIDIFFYNLIEGSYNEEIMRYVENAMYIPRQSKKFLGMVANAFGGSSLKRIGDVIDEYINDRLRDMEIAMDGDIERVLDEISTEIQKRGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMT1</accession>
    <name>I7AMT1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RSE1/DDB1/CPSF1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264546.1">
        <property type="nucleotide sequence ID" value="XM_009266271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMT1"/>
    <dbReference type="GeneID" id="20521350"/>
    <dbReference type="KEGG" id="ero:EROM_051180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051180"/>
    <dbReference type="HOGENOM" id="CLU_305879_0_0_1"/>
    <dbReference type="OrthoDB" id="2190953at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="1">
        <location>
            <begin position="727"/>
            <end position="914"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="969" mass="109486" checksum="938FE9834D60CD0B" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMZ0</accession>
    <name>I7AMZ0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="5">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_060240</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264616.1">
        <property type="nucleotide sequence ID" value="XM_009266341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMZ0"/>
    <dbReference type="GeneID" id="20521421"/>
    <dbReference type="KEGG" id="ero:EROM_060240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060240"/>
    <dbReference type="HOGENOM" id="CLU_1288890_0_0_1"/>
    <dbReference type="OrthoDB" id="2530521at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051370">
        <property type="entry name" value="PPIase_Pin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10657:SF4">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00639">
        <property type="entry name" value="Rotamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="6">
        <location>
            <begin position="105"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="214" mass="24739" checksum="BE6A45DECF72F249" modified="2012-10-03" version="1">MSTCPKNILRQHILLIPSSSSASASFLSVLRSSRYGSHLLEISFPQVKHLIGIIILERARILTLNGYNSYIRLYPEMWIKLKDKETGQPYFYNTETREKTEKKPMEGFRLYHILIKHEKSRKPVDVSIDEALSRIKTIYEDLKAKVNDKNFKDLFKEAAFKHSQCSSAKRGGDLGFVCGNEMMKEFERPAFSLRKGEMSEPVSTPSGFHIIYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN44</accession>
    <name>I7AN44_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264641.1">
        <property type="nucleotide sequence ID" value="XM_009266366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN44"/>
    <dbReference type="GeneID" id="20521447"/>
    <dbReference type="KEGG" id="ero:EROM_060500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060500"/>
    <dbReference type="HOGENOM" id="CLU_1124525_0_0_1"/>
    <dbReference type="OrthoDB" id="2193825at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="246" mass="27920" checksum="E9E192428B55B19B" modified="2012-10-03" version="1">MDSSMKKRFIELLSIYGSDFDAIASTNELDGMTSEMLYSKYPEILDTYFLKNTQLNQDEINWLQIKEIYYMNERFYFDVLIGELCGSHVIKNGRDLEIGKELVLLLKDYEFKLLSKLSILDKLYKSLHKDGMLSEDLSRRISTSSSKSMEKKNKSKASPSVEDSESKKKLSNIAVVVKSSQEISSAKDGAEDGSSAKDTKDSIEKQAEEIEEVAEQCPSDRKPANKGNTMGLDYSAVLRLSREQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN59</accession>
    <name>I7AN59_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Symplekin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264661.1">
        <property type="nucleotide sequence ID" value="XM_009266386.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN59"/>
    <dbReference type="GeneID" id="20521467"/>
    <dbReference type="KEGG" id="ero:EROM_060710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060710"/>
    <dbReference type="HOGENOM" id="CLU_471800_0_0_1"/>
    <dbReference type="OrthoDB" id="331600at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021850">
        <property type="entry name" value="Symplekin/Pta1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022075">
        <property type="entry name" value="Symplekin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245:SF20">
        <property type="entry name" value="SYMPLEKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15245">
        <property type="entry name" value="SYMPLEKIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12295">
        <property type="entry name" value="Symplekin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Symplekin C-terminal" evidence="1">
        <location>
            <begin position="404"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="592" mass="69211" checksum="4032C06D061E1CC1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANI0</accession>
    <name>I7ANI0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ski2-like ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_070780</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264826.1">
        <property type="nucleotide sequence ID" value="XM_009266551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANI0"/>
    <dbReference type="GeneID" id="20521637"/>
    <dbReference type="KEGG" id="ero:EROM_070780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070780"/>
    <dbReference type="HOGENOM" id="CLU_004167_0_0_1"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003287">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="115"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="316"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1059" mass="122203" checksum="30657763339601FC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANP9</accession>
    <name>I7ANP9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Probable phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="5">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_071580</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="5"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264906.1">
        <property type="nucleotide sequence ID" value="XM_009266631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANP9"/>
    <dbReference type="GeneID" id="20521722"/>
    <dbReference type="KEGG" id="ero:EROM_071580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071580"/>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_1"/>
    <dbReference type="OrthoDB" id="238316at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000178">
        <property type="entry name" value="Phenylalanyl-tRNA synthetase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040725">
        <property type="entry name" value="PheRS_DBD3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18553">
        <property type="entry name" value="PheRS_DBD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="17">
        <location>
            <begin position="206"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN83409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="472" mass="54272" checksum="60A94213D4D6D86A" modified="2012-10-03" version="1">MVQKIVEVLKEQDEVRSSDIDAPYSEIHGALISLESKGICDVRKEESYQEVLTPEGEEVEKNGSPEYLFFKSIGKDGIDSDELSKHEVGKMNAFKNKWVKRKDGKIFRSVEKVEDMIRNMVINMKRGKATDEEIEVLRKRKLVIRKKNIVFVATKGPMFFDNTSYATELTSGMVLDGSYKSLTFKHYNFDAKGNIPQCGSIHPLMKIREDFKRIFIELGFSEMPTDQYVESSFWNFDVLFQPQNHPSRDAHDTFFLLNPELSLNFPKDYLNEVKETHCGGKYGSLGYMNNWDIKEAQKNILRTHTTSVSARNLYKLAKEGFKPVKLFSIDKVFRNESVDATHLAEFHQVEGLIAGKGLNITHLMGILQEFFNRLGMCDIRFKPAFNPYTEPSMEVFGYHKGMNRWMEVGNSGIFRPEMLGPMGFDSDVSVIAWGLSLERPAMIKYGLKNIRELVGHKVDIEFSRKSEICFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7APJ2</accession>
    <name>I7APJ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265266.1">
        <property type="nucleotide sequence ID" value="XM_009266991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APJ2"/>
    <dbReference type="GeneID" id="20564377"/>
    <dbReference type="KEGG" id="ero:EROM_091530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091530"/>
    <dbReference type="HOGENOM" id="CLU_704041_0_0_1"/>
    <dbReference type="OrthoDB" id="2200771at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012468">
        <property type="entry name" value="DUF1686"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07937">
        <property type="entry name" value="DUF1686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="392" mass="44678" checksum="9FC553B253ADC38B" modified="2012-10-03" version="1">MSLQPKVQMKANQASEDKKEEVEELLCNIREGLVTLVQNLSNFKDIYGKGLDDIISNERIFETLICTGETLGKVSPFLASLEKNIKSFEQKASLPKGAIYEEMYLSLAKRGNIRIGVPIFPTYTLYNLLMAISTLEKMNEVLGDIVKPEKDRGVKRPIADLYLVFVFVLCIQTLLYYITQKHPKVWKVCNTTLYSLTVGGSILYLVWVVIKSYRSEGRMKEILRQRLSGVISMIPMVVGIHHTEMIECGTYTLTMMGISAIMVYAQDLVENKMTWRQICVVGVRNAILAIAYCCEILRMFMRNEYRLRCCVCGGVVIFVLLVGILCYSSSMKEGSKTRLPGNVMFVLTVVIGTFVSWRSRNEYYHLRSRDGYIEGMEEARYDSIKGRVSCDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7APP1</accession>
    <name>I7APP1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">inorganic diphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_100220</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265336.1">
        <property type="nucleotide sequence ID" value="XM_009267061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APP1"/>
    <dbReference type="GeneID" id="20564452"/>
    <dbReference type="KEGG" id="ero:EROM_100220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100220"/>
    <dbReference type="HOGENOM" id="CLU_040684_0_2_1"/>
    <dbReference type="OrthoDB" id="1608002at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="277" mass="31900" checksum="330762FA6BDFD50D" modified="2012-10-03" version="1">MVNYSTVRVGKKYSPSFKVYVAQGGKIVSPFHDVPLYMSENRDVVSVVNEIPRFENGKFEINKEERFNPIKQDSKNGWPRFVKNVFPMKGYLWNYGALPQTWENPHEIDKHVGAKGDNDPLDVIEIGARRKEIGEVYQAKVLGSIALVDEDECDWKVVVIDVNDEKAKEINDIEDVRKAYRGLLEQTIFWFENYKVPDGKSKNSFALDGKYMNKEFTVNIVKSAHENWCGMVNSKGNDSICKENSTLMDKIDPPMISHEELSDEEAPEYINQFEFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7APW4</accession>
    <name>I7APW4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263849.1">
        <property type="nucleotide sequence ID" value="XM_009265574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APW4"/>
    <dbReference type="GeneID" id="20520633"/>
    <dbReference type="KEGG" id="ero:EROM_010070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010070"/>
    <dbReference type="HOGENOM" id="CLU_1758788_0_0_1"/>
    <dbReference type="OrthoDB" id="2191125at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="3"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="151" mass="17886" checksum="A26D510C58FC05EF" modified="2012-10-03" version="1">MFCIPCQKKFSTKNAYKCHILTSSHAKAEDEYKINRKVAIERNTVNFITDFHSYISQIAEYKEIGQAYREYLTKNRFRIEGTRFKSVEEAVDKIAKRISVMKEEGRIMIKRLSKFESVKEPPAFDLEKLSSTFRIRVVKKNDRYVEESLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ36</accession>
    <name>I7AQ36_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ser/Thr protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_110050</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265486.1">
        <property type="nucleotide sequence ID" value="XM_009267211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ36"/>
    <dbReference type="GeneID" id="20564604"/>
    <dbReference type="KEGG" id="ero:EROM_110050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110050"/>
    <dbReference type="HOGENOM" id="CLU_000288_5_15_1"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14133">
        <property type="entry name" value="PKc_DYRK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF17">
        <property type="entry name" value="HOMEODOMAIN INTERACTING PROTEIN KINASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="8">
        <location>
            <begin position="40"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="405" mass="45883" checksum="028118668F43E977" modified="2012-10-03" version="1">MNRKVLTFPSERIGLSPDNAEFDLVMARGDYLSKNKNQKYMVIGMLGTGTFGQVVRCVGPDGEEVAIKVVKNQPKYYNYEMNEVRILHKLLYNNLNDRFVAIKDVFMYKQHLCIVEELLGRNLYTFLKMTRFKGLDHSTVRTILKQVLEGMIQLSVLGIIHCDLKPENILIADYDTFKVKIIDFGSAVTSPQVSHFYVQSRYYRAPEVILGIPYGSSCDIWSLGCIGYELYVGNPLFPGKDNMDQMGRIYGLLGSLPMFMIEHGKNSNVFFEKENGYRFVGPTSGFTLEDMKKVIRSKGGLEGDDDMFIEFLLKTLQPSHLIRPDARSLSGHSYLKLKDEGGDLCKAPSGRKMVQQSVPPTSKGIRHMSTTGVILPSKKQKSGDERRKISVYGISYENNINRDSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ40</accession>
    <name>I7AQ40_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Triosephosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_110100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265491.1">
        <property type="nucleotide sequence ID" value="XM_009267216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ40"/>
    <dbReference type="GeneID" id="20564609"/>
    <dbReference type="KEGG" id="ero:EROM_110100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110100"/>
    <dbReference type="HOGENOM" id="CLU_024251_2_0_1"/>
    <dbReference type="OrthoDB" id="6715177at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF2">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="3 4 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="242" mass="26982" checksum="0BF9B15D7D99D2C5" modified="2012-10-03" version="1">MKKNKLLGGNWKMNSSLQTFDLVKNFRKNFFDGNDAFIAVPFPYIHIAKEIFPEYIKVGAQDCSQFINGPYTGEVSASMIKEMGAEYVIVGHSERRKWFGETSTVISKKIRNARNQGLNVILCIGEDEKCREDGGYLDALKDQLLSLKEDISGEDIDVAYEPVWAIGTGKVPTNRQISEVISSIKNWGKDLGFYGRVIYGGSVSILNCQTILDVEEVDGFLIGGTSLKMDFCMISSEMGTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ65</accession>
    <name>I7AQ65_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chitooligosaccharide deacetylase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265516.1">
        <property type="nucleotide sequence ID" value="XM_009267241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ65"/>
    <dbReference type="SMR" id="I7AQ65"/>
    <dbReference type="GeneID" id="20564636"/>
    <dbReference type="KEGG" id="ero:EROM_110370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110370"/>
    <dbReference type="HOGENOM" id="CLU_1082481_0_0_1"/>
    <dbReference type="OrthoDB" id="407355at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004099">
        <property type="term" value="F:chitin deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009272">
        <property type="term" value="P:fungal-type cell wall biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10917">
        <property type="entry name" value="CE4_NodB_like_6s_7s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050248">
        <property type="entry name" value="Polysacc_deacetylase_ArnD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587">
        <property type="entry name" value="GLYCOSYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10587:SF125">
        <property type="entry name" value="POLYSACCHARIDE DEACETYLASE YHEN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="23"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="249" mass="27841" checksum="5B9B0AF0C91622C1" modified="2012-10-03" version="1">MLWILYFASCWCEDVPDVCTTSGMIAINFVDGPVRGVTDRVLNTLDELGVKATFSFTVNQKAVGNVGQLYRRAVNEGHTVGLRIDPRMDDGCQNLSRDALEENVDREIDTIDGLSGTEVRYASVPINNGEVNSEMYSILTERGVLPLGYTFCPYDYDSPIEEFECMIEGSDPKHHSFIILMHDGQEGDTTRLERMVKIGEDKGYRFVNMDECLKGYKGAPGEPEIALRGKSAQNIATFLPFFMLLARLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ67</accession>
    <name>I7AQ67_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_011130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263954.1">
        <property type="nucleotide sequence ID" value="XM_009265679.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ67"/>
    <dbReference type="GeneID" id="20520740"/>
    <dbReference type="KEGG" id="ero:EROM_011130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011130"/>
    <dbReference type="HOGENOM" id="CLU_000288_103_6_1"/>
    <dbReference type="OrthoDB" id="190105at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057780">
        <property type="entry name" value="Beta-prop_Vps41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23411">
        <property type="entry name" value="Beta-prop_Vps41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="175"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="214"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Vps41 beta-propeller" evidence="5">
        <location>
            <begin position="220"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="293"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="408" mass="46401" checksum="A13F42F075CA0A52" modified="2012-10-03" version="1">MNNKEKAKPDKNRYGVLNLPQELIPRLIAHLSVKHIKRLMGCNSLLYIKLRNDFSLWRFFTSGRHSSIAGYIEEIKNKYTLMRNISRSKNERSIDFETNQSDLTFMQIDRDRIICSSDDQTIKIFGMNGRLIRTFIGHKGGVWTFMFNSKYLVSGSTDKTARIWDLWTGCTLCVLVGHKSVVRSLKIYEDYIATGSRDSEIRVWNFRGTCLNVLKGHTMSVRCMDMDESYLVSGSYDGSVALWDYRKGKLLRYFKSHSLRVYSVSLSPSYVASGSLDSSVHISALDGRLLCSYKIHRSLVIWLKFVGNGRYLLSSGADGILCKWDVLENALVYKIEENGHITAQAVVEDLLIVGTKREVKIYNLSSGQFIRTLFSASSLISKVEVSGRCISIGYYSDSGACRLVVFNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQE6</accession>
    <name>I7AQE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleic-acid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020620</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264034.1">
        <property type="nucleotide sequence ID" value="XM_009265759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQE6"/>
    <dbReference type="GeneID" id="20520822"/>
    <dbReference type="KEGG" id="ero:EROM_020620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020620"/>
    <dbReference type="HOGENOM" id="CLU_034868_0_0_1"/>
    <dbReference type="OrthoDB" id="2189352at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="419" mass="48187" checksum="A4AE684BBF5E2479" modified="2012-10-03" version="1">MAKPKDGGEKKASRNSSKHGGSKSRGETKKKIVLKRKPCPIQGKVFPDASKMEKDLLVHGTIKDKIDVLTLLTERNPSAENYKGLLAFCENQRNDVHYYALKNIKDLLISKREVGNPYIKQRIVKSFGVNLRNQYIKKKVIRLVEKLLEKGVLFVDLIHLFINKLGDKKDTTSNVIDKLHLLFPGNEETILDGVEDFYFKNDLFRSRHVVLKFLSKLDCENKRKAFEVFNNILRDFNENIPEEHRNILLEDLIIGLGKNMTDEKVCRMDIIRKAVHTEKMIFYGSKVLSNCKDSEMILFFKNAIRSYKMRNSKHLPEFLNMVHKAVSADEKIEFHKFLLDTSLLYTPEYIISILIICSEARKAGISGFDNIHSLRILALHHNDAVRGLALQLISDEAIHPFDPFDKMSLLNAEMMCGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQK9</accession>
    <name>I7AQK9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_021320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264104.1">
        <property type="nucleotide sequence ID" value="XM_009265829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQK9"/>
    <dbReference type="GeneID" id="20520894"/>
    <dbReference type="KEGG" id="ero:EROM_021320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021320"/>
    <dbReference type="HOGENOM" id="CLU_021695_0_2_1"/>
    <dbReference type="OrthoDB" id="9446342at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF8">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="429" mass="49245" checksum="C493DD1993FA5CDF" modified="2012-10-03" version="1">MITDHEYDFVILGTGLVECAVGCILAGKNKKVILIDRNPMYGSDFATLRYTELETYFRNLNTIPELKTYDSEFSIDLTPKLFLADSKMLKMLVRYGIDEYLEFCRIPGSFLWKKKLYSVPTNEAQSMTTGLIGIWQKPKVMRFFWNVRDYARAAMKGKPYKFKNTMREEFKEYGLTEESMELIGHGIALNLDDSYLDRDPRETFDKIVTYVRSIICYENSMESPYLYPRYGLSEIAQGFARSCCTKGGEIMINAEILEINEENMEIHVQEPVNKKVLKIKAGKIISDQSYFQKSVVLYEIIRGICVIRGDAACVTRGASSAQIIFLKGELGRKNDIFVVILGPEEKSTPEGYKVAIISTVKETDDPEGEIRVVLEKLGDIVRYFVDVKPVYRAEDMENIIFTKGVDESPHFEDVYEEIERICKKLNVCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQT2</accession>
    <name>I7AQT2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Hsp70</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030410</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264159.1">
        <property type="nucleotide sequence ID" value="XM_009265884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQT2"/>
    <dbReference type="GeneID" id="20520952"/>
    <dbReference type="KEGG" id="ero:EROM_030410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030410"/>
    <dbReference type="HOGENOM" id="CLU_005965_0_1_1"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd24028">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000012">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000010">
        <property type="entry name" value="heat shock 70 kDa protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="682" mass="74634" checksum="8BB4D3A1702939A1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQX9</accession>
    <name>I7AQX9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homeodomain-containing transcription factor-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_031050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264224.1">
        <property type="nucleotide sequence ID" value="XM_009265949.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQX9"/>
    <dbReference type="GeneID" id="20521018"/>
    <dbReference type="KEGG" id="ero:EROM_031050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031050"/>
    <dbReference type="HOGENOM" id="CLU_106359_0_0_1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF9">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3 4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="32"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="3">
        <location>
            <begin position="34"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="227" mass="26809" checksum="230DD20D77F74B85" modified="2012-10-03" version="1">MDNFGSSFFNSYTNNSNREAEGGQYFDPYFVKHRKRTTKAQLKVLEETFETNIRPDANMRKKLGEQLGMTPRSVQVWFQNRRAKIKKLTQKKMMQQENTDNTKGPEASRSSSPSKDRKYSNYHPYVPLQAPLEEFNVYPRDNSVYKHSIPQGMSSPIMYEGFGYRGHEEYGYPYVQRYGNGEYYQMQYPYGMAQAQWHQPPGFRDEDYYHSTRKQQYRGGAPNPEKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQZ7</accession>
    <name>I7AQZ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_031350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264254.1">
        <property type="nucleotide sequence ID" value="XM_009265979.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQZ7"/>
    <dbReference type="GeneID" id="20521049"/>
    <dbReference type="KEGG" id="ero:EROM_031350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031350"/>
    <dbReference type="HOGENOM" id="CLU_1643690_0_0_1"/>
    <dbReference type="OrthoDB" id="2191908at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="161" mass="19156" checksum="9FF20BE480D6A625" modified="2012-10-03" version="1">MYVRIGRINKIVNRHDLHKIFTFTRPIYKSIECSSGIQLYFYSTLHEIVMAQQLNNENLIERLEVCFIEYTLEEELCLSISSSRPSCCLYAICDGDVCIDMIQRFFGSPIKCYLRTGRSKERYLVEFKEQVEVRKPDFMRCTLSFEDYIDYIVRAGIYDYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARC6</accession>
    <name>I7ARC6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">mRNA-capping enzyme subunit beta</fullName>
            <ecNumber evidence="8">3.6.1.74</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">mRNA 5'-triphosphate monophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_041580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">First step of mRNA capping. Converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate end.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end diphospho-ribonucleoside in mRNA + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67004"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
            <dbReference type="EC" id="3.6.1.74"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67005"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer. The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an mRNA 5'-triphosphate monophosphatase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the fungal TPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.74" evidence="8"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264419.1">
        <property type="nucleotide sequence ID" value="XM_009266144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARC6"/>
    <dbReference type="GeneID" id="20521221"/>
    <dbReference type="KEGG" id="ero:EROM_041580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041580"/>
    <dbReference type="HOGENOM" id="CLU_1245346_0_0_1"/>
    <dbReference type="OrthoDB" id="272147at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031533">
        <property type="term" value="C:mRNA capping enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140818">
        <property type="term" value="F:mRNA 5'-triphosphate monophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004651">
        <property type="term" value="F:polynucleotide 5'-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.10">
        <property type="entry name" value="mRNA triphosphatase Cet1-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040343">
        <property type="entry name" value="Cet1/Ctl1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004206">
        <property type="entry name" value="mRNA_triPase_Cet1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037009">
        <property type="entry name" value="mRNA_triPase_Cet1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118:SF1">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE CTL1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28118">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-TRIPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02940">
        <property type="entry name" value="mRNA_triPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="4 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="mRNA triphosphatase Cet1-like" evidence="9">
        <location>
            <begin position="92"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="222" mass="26042" checksum="361628C309F7F62D" modified="2012-10-03" version="1">MEEDLTKILKGPVKDHSEYLRKFILAFKNERKELEIRLGKIISKEDGSRMRVDTVAPVVFRSIPMDYRFESAVDLSDFNILKKQFSTLKSEAKNDVVIINEDGRETYEDGVLKKVEKKTRSQKLDIFIPGKKYDVRLVLSEENEGFKRASKKKPIVKRVRRRETYFIEPYGFDFTEAILSGDKDEKEKRKFEIEVEVFSSEKLVSNDFVSLIYNFNVDLCTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARL0</accession>
    <name>I7ARL0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264509.1">
        <property type="nucleotide sequence ID" value="XM_009266234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARL0"/>
    <dbReference type="GeneID" id="20521312"/>
    <dbReference type="KEGG" id="ero:EROM_050800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050800"/>
    <dbReference type="HOGENOM" id="CLU_1085977_0_0_1"/>
    <dbReference type="OrthoDB" id="2190866at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="256" mass="30380" checksum="866FCF277E4A4EBF" modified="2012-10-03" version="1">MLRLYEGMIFVPYSPPECTFVQIIHRKTIRILPGEKYNIPVCLNCRPVFDNIYVHTGESSLMTVNYIQTYTSEEGRGILSYREFQDIKVTRQKILRSEIYEVYPPFSIREYFDGTFLFLKLEELFEEGAIKKYEVYSERNERLKGNGSIYKIYPDRRYDQEYVILGEEFVFRFQSRCVFACPFYFNFWAAEETVYFVAKARNRVLEVECPGLASSDTSKGMISLPLDQFNKQGRFLYIGDYSLRFSPEFYNHCDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARL5</accession>
    <name>I7ARL5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Grd19 sorting nexin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264514.1">
        <property type="nucleotide sequence ID" value="XM_009266239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARL5"/>
    <dbReference type="GeneID" id="20521317"/>
    <dbReference type="KEGG" id="ero:EROM_050850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050850"/>
    <dbReference type="HOGENOM" id="CLU_163495_0_0_1"/>
    <dbReference type="OrthoDB" id="2189967at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06093">
        <property type="entry name" value="PX_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775">
        <property type="entry name" value="SORTING NEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22775:SF3">
        <property type="entry name" value="SORTING NEXIN-13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="1">
        <location>
            <begin position="2"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="120" mass="14519" checksum="3CF320ABDB75550B" modified="2012-10-03" version="1">MEEERLLEITIPGSRNVQDRYTEYEVVCITNNKSFGKCYTKVFRRYRDFHMLHSRLKCFLKVLPEFPRKRWNKMDKDVIEERMRMLTVYLKFVCDEVLKGRKDMEKVEADIVAFIQGKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ART0</accession>
    <name>I7ART0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">U three protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_051570</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264584.1">
        <property type="nucleotide sequence ID" value="XM_009266309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ART0"/>
    <dbReference type="GeneID" id="20521389"/>
    <dbReference type="KEGG" id="ero:EROM_051570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051570"/>
    <dbReference type="HOGENOM" id="CLU_022996_1_1_1"/>
    <dbReference type="OrthoDB" id="10251154at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000378">
        <property type="entry name" value="U3 small nucleolar RNA-associated protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012952">
        <property type="entry name" value="BING4_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040315">
        <property type="entry name" value="WDR46/Utp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085:SF3">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14085">
        <property type="entry name" value="WD-REPEAT PROTEIN BING4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08149">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01033">
        <property type="entry name" value="BING4CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="232"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="BING4 C-terminal" evidence="9">
        <location>
            <begin position="315"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00076453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="433" mass="49697" checksum="92BB33FB46F5C77D" modified="2012-10-03" version="1">MSKKIIEEKIKSRRKIVEKAHRDVSSYDILRTESCGYIEVDSDEKTYDATQSKIRRHVSIKACEQAYDLRLDNGPIHAKYSRNGMHMLLRNDRGYLASFNTKSMNLHFEIDVNDKMYDAIYLHNERFIAVAQKNNVFIYDGCGVETHCVRDNSDVFKMEYLPYHYLLVSVSRRGFLRYQDISTGNFVSQILMKNQEVISIKQNPMNAIIHTGSTKGVVSLWSPSSKEYLMKILCHKSAISSIEIERDGRSMVTMGMDNRINVWDLRNTYRQLNTLRAKSGLIATSLSQKSMLALSYGGNVHIWKDFINSDSGEVLYIKHKVGSLVSSVDFCNHEDILCIGHANGISSIIVPGCGDPVYDSYEDSPFTSKKARREKEVRRLMEKIPYELISMESKVGSIYKEPKVDKPKEEFSRYFDAEPVRKGALSRFYNKDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS68</accession>
    <name>I7AS68_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Anti-silencing function protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_070060</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ASF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264754.1">
        <property type="nucleotide sequence ID" value="XM_009266479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS68"/>
    <dbReference type="GeneID" id="20521564"/>
    <dbReference type="KEGG" id="ero:EROM_070060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070060"/>
    <dbReference type="HOGENOM" id="CLU_083062_0_0_1"/>
    <dbReference type="OrthoDB" id="29755at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1490">
        <property type="entry name" value="Histone chaperone ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006818">
        <property type="entry name" value="ASF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036747">
        <property type="entry name" value="ASF1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040">
        <property type="entry name" value="ANTI-SILENCING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12040:SF0">
        <property type="entry name" value="HISTONE CHAPERONE ASF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04729">
        <property type="entry name" value="ASF1_hist_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101546">
        <property type="entry name" value="ASF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="124"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="266"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="292" mass="33236" checksum="5CB34AF7771615F4" modified="2012-10-03" version="1">MGYLKLKSVKVDSTTKKLVDPLKFNFRFACSNEIKSGVEFTVLYNMDVHSDENDQVLAEVEIAPIPKGKIEFSIDADAPDVNKIPLDDIFGLTSILIIGRYKGQQFIRIGYIVDVGYPGIPSSKLIKNDIEEPSEEIEDKEEDEEDVEENSESIEGSDESSYVVKDEDENAEEAKPRTFMEAVEDVVDEGKERIFESEGREEEENEKVGSDSYSEINRELNKNIGEEEVKASNGDEDVFDYCGYKINKKEIEMKLMDPPVINLFEIEWSDETPDKERLDTNDESPAKKQKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASE0</accession>
    <name>I7ASE0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L37A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070960</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264844.1">
        <property type="nucleotide sequence ID" value="XM_009266569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASE0"/>
    <dbReference type="GeneID" id="20521656"/>
    <dbReference type="KEGG" id="ero:EROM_070960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070960"/>
    <dbReference type="HOGENOM" id="CLU_141199_1_0_1"/>
    <dbReference type="OrthoDB" id="2185952at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="89" mass="9830" checksum="41D70F6E1EB24D23" modified="2012-10-03" version="1">MGKGTKKVGIAGSFGARYGSTLRKRMKAVLETQRAKYSCEACGKKRVRREAVGIWKCRSCRRIFAGGSYVTSTPAGKIFINMVKQHNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASJ5</accession>
    <name>I7ASJ5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">26S proteasome regulatory subunit 4 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_071560</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264904.1">
        <property type="nucleotide sequence ID" value="XM_009266629.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASJ5"/>
    <dbReference type="GeneID" id="20521720"/>
    <dbReference type="KEGG" id="ero:EROM_071560"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071560"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_3_1"/>
    <dbReference type="OrthoDB" id="10255768at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000030">
        <property type="entry name" value="26S protease regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000039">
        <property type="entry name" value="26S proteasome regulatory subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7 13" id="KW-0647">Proteasome</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="12">
        <location>
            <begin position="202"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="424" mass="47105" checksum="75C19737243D88B1" modified="2012-10-03" version="1">MSGDKPEEDNLQDTTTINLKRRKRVKESKTASKPPQVYPQMKCKLRCLKLGKLAHLLSLEDNILSLCEPDKSSEGSNSQKHVVEQLRGSPLSVGTLEEFIDDHHGIITTGVGLEYYVNIMSFVDKDLLEPGCTVLLNYKDNSVVGVLEGEMDPMVNVMKLEKAPSETYADIGGLEDQIQEIKESVELPLTNPELYQEMGIKPPKGVILYGLPGTGKTLLAKAVANQTSATFLRVVGTELIQEYLGEGPKLVRELFRVADMHAPSIIFIDEIDAIGGRRYNTSSGGRREVQRTMLELLNQLDGFDTRNDIKVIMATNKIEALDPALIRPGRIDRKIEFGMPDAATKKKIFDIHTSKMTLDESVNIESLITSKEDLSGADIKAICTEAGMIALRERRKTVTMKDFISAREKVFFSKQKVISAGLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASL6</accession>
    <name>I7ASL6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Vesicular transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264929.1">
        <property type="nucleotide sequence ID" value="XM_009266654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASL6"/>
    <dbReference type="GeneID" id="20521745"/>
    <dbReference type="KEGG" id="ero:EROM_080090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080090"/>
    <dbReference type="HOGENOM" id="CLU_540895_0_0_1"/>
    <dbReference type="OrthoDB" id="2189189at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="466" mass="54848" checksum="44C019EB54C44D81" modified="2012-10-03" version="1">MFFKKHVEPKPLRVEEELGLLSNRLSGSIYYEDRADALSKILEMSRTYPVEVGVYTLQDVIHSMEKMEDISIHLDILSNVLRCTHRLEFIDIVVKNPETLRILCDCIKCGKKEKEVYDLLCILSTSELFPRRAVGIPGMAYHCVQMAKEKKMRLIPRLVEQDQNFKRELTFMGIFENLLKVLQDGFFKDAMSTLVLLLRDCPFNQNYFDELKWDFILNFIDKHPGEVFDVLSCLMDPKNTEFKKIQTSIYGKVDLRLVLKFKRWSLLYLIVKDNKSYTEKLLENFVFDKIEEELSKEAFVRKRNEIYLLVDYLLFWNDFDASKLDSYKIYTMKSLREQHISTNDLIERAFETICQFDNKEEGASFDALIFIIFNFEKTKAEKMIPTLSEIFGDYTRPKLHRSLCLIILLMLEINVDRIGINHYTADHMLREARLLLCSIDLESPLYLTNEMVDILVSSIGDLIRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASV8</accession>
    <name>I7ASV8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_081160</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265029.1">
        <property type="nucleotide sequence ID" value="XM_009266754.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASV8"/>
    <dbReference type="GeneID" id="20521851"/>
    <dbReference type="KEGG" id="ero:EROM_081160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081160"/>
    <dbReference type="HOGENOM" id="CLU_010194_38_0_1"/>
    <dbReference type="OrthoDB" id="5545019at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051019">
        <property type="entry name" value="VLCFA-Steroid_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43899:SF13">
        <property type="entry name" value="RH59310P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="33375" checksum="FE57E1981DD93F90" modified="2012-10-03" version="1">MVIQLIYLGVSYVLLKVVPIVYQYIYSKCRNHQVWEGIRKKYVLINGATGKVGRGLAKKFAEKGMKILLVGRSESRLTRLHAKIERMTECHMHIVDYKRSPNISFVDKYDIGILVNCASAMKDSPAYFVEQSVEEIMNANLVGVMSLTRYVLVNMMDCGYGYVINVGSLMGEASTPLYSAFGCSKQALKALSDSLYYELEGYNVNVEYMGVGSVSSGEDGEKPSLLRPSSDTLAESILNTFGSAKMSVPYFPHFILYLIMLCIPSPLLSRIILRRNKKEMIEVRNRELNLENQKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASY9</accession>
    <name>I7ASY9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">V-type proton ATPase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the V0 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments. This subunit is a non-integral membrane component of the membrane pore domain and is required for proper assembly of the V0 sector. Might be involved in the regulated assembly of V1 subunits onto the membrane sector or alternatively may prevent the passage of protons through V0 pores.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265064.1">
        <property type="nucleotide sequence ID" value="XM_009266789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASY9"/>
    <dbReference type="GeneID" id="20521886"/>
    <dbReference type="KEGG" id="ero:EROM_081510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081510"/>
    <dbReference type="HOGENOM" id="CLU_846078_0_0_1"/>
    <dbReference type="OrthoDB" id="10250083at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016727">
        <property type="entry name" value="ATPase_V0-cplx_dsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11028">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018497">
        <property type="entry name" value="V-ATP_synth_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR018497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="330" mass="39477" checksum="229943D574220D5B" modified="2012-10-03" version="1">MEKQFNHVERYGYIISEINGKREEMLKEEDYNALKRCESLEEVAIKLSKTYRGLSEGIAYTKPELKKKLLESLKADFDYYHDAEDKGIRTILDYYMDFHKIQNFFYLLQCKLQDPNLEKSFEKIEIGDFSALRTIKFSNDMDDVQRYCMENSFLKKFEKRVRFKKEFSANNFQVLQTLFFKFHIEETYRNLNDDMEYMKEILRLEGDRQIIEITVNTLNSKDLVGKKRMSLFPDVHSMSLNARELLSHVENLEDMKSILSEAYNFDTDISNVLIKAELQKYQESFSMYGDLSCVYSYFKMKEQEIKNILWVAECIVQNRRDAMDHLFVVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT33</accession>
    <name>I7AT33_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">60S ribosomal protein L12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081980</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN83612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266834.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20521933"/>
    <dbReference type="KEGG" id="ero:EROM_081980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081980"/>
    <dbReference type="HOGENOM" id="CLU_074237_5_0_1"/>
    <dbReference type="OrthoDB" id="1478556at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_L11"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
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    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="6">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="5">
        <location>
            <begin position="74"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="166" mass="18037" checksum="C37902B5110725B8" modified="2012-10-03" version="1">MAGQKVIDPDTKYIKLQVVGGELPGATLAQRVGPLGLSSKVVGEDIRKATTDYKSLKVHVELAIKDRKATVEVQPSIATLIIKALKEPPRDRKKEKNILHNGSLKMTEIVEIARIARSSRSYSKTLAGAVKEVLGTCKSIGCKVDGKCPKEVTHEINSGEIRLPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATK4</accession>
    <name>I7ATK4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_091820</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009267019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATK4"/>
    <dbReference type="GeneID" id="20564406"/>
    <dbReference type="KEGG" id="ero:EROM_091820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091820"/>
    <dbReference type="HOGENOM" id="CLU_327609_0_0_1"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027351">
        <property type="entry name" value="(+)RNA_virus_helicase_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014808">
        <property type="entry name" value="DNA_replication_fac_Dna2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08696">
        <property type="entry name" value="Dna2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01443">
        <property type="entry name" value="Viral_helicase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication factor Dna2 N-terminal" evidence="10">
        <location>
            <begin position="43"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="DUF83" evidence="9">
        <location>
            <begin position="246"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="(+)RNA virus helicase C-terminal" evidence="8">
        <location>
            <begin position="586"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="510"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="878" mass="100821" checksum="28CBBD43EF0B0463" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUE8</accession>
    <name>I7AUE8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cell division control negative transcription regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265604.1">
        <property type="nucleotide sequence ID" value="XM_009267329.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20564725"/>
    <dbReference type="KEGG" id="ero:EROM_111260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111260"/>
    <dbReference type="HOGENOM" id="CLU_004563_0_0_1"/>
    <dbReference type="OrthoDB" id="1933107at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
        <property type="term" value="C:CCR4-NOT core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20710">
        <property type="entry name" value="NOT1_connector"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.840">
        <property type="entry name" value="CCR4-NOT transcription complex subunit 1 TTP binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055454">
        <property type="entry name" value="CNOT1-like_NOT1_connector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032191">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024557">
        <property type="entry name" value="CNOT1_dom_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032194">
        <property type="entry name" value="CNOT1_HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032193">
        <property type="entry name" value="CNOT1_TTP_bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038535">
        <property type="entry name" value="CNOT1_TTP_bind_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040398">
        <property type="entry name" value="Not1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13162:SF8">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25097">
        <property type="entry name" value="ARM_Cnot1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16415">
        <property type="entry name" value="CNOT1_CAF1_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16418">
        <property type="entry name" value="CNOT1_HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16417">
        <property type="entry name" value="CNOT1_TTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12842">
        <property type="entry name" value="DUF3819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 HEAT repeat" evidence="4">
        <location>
            <begin position="219"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 TTP binding" evidence="3">
        <location>
            <begin position="388"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1 CAF1-binding" evidence="2">
        <location>
            <begin position="615"/>
            <end position="740"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1" evidence="1">
        <location>
            <begin position="769"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="domain" description="CCR4-NOT transcription complex subunit 1-like NOT1 connector" evidence="5">
        <location>
            <begin position="1018"/>
            <end position="1103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1460" mass="169160" checksum="350BB743FADB6B31" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGS5</accession>
    <name>I6ZGS5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010430</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263885.1">
        <property type="nucleotide sequence ID" value="XM_009265610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGS5"/>
    <dbReference type="GeneID" id="20520670"/>
    <dbReference type="KEGG" id="ero:EROM_010430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010430"/>
    <dbReference type="HOGENOM" id="CLU_047996_0_0_1"/>
    <dbReference type="OrthoDB" id="2193759at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031505">
        <property type="entry name" value="DUF5098"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17023">
        <property type="entry name" value="DUF5098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="457" mass="52194" checksum="31C356EB2ADEC1CC" modified="2012-10-03" version="1">MEDVENIKASYKMLKCKCVYLKSYLEHIIIVSKRSSDLLFRALQIPQDTAWNDPLLMHCIEMSERQRAVFDEAGLLYGETLFKELDGLIATIVSDIRMIDEEVKRVSEKPAEGLKELRIARNKHMEAWTYGKHDPWLTELGLKRAVKKVLLMDDEANHVMYDKIGGFAESLQGAGERFKEALLSLVRIQRQKYQKLRDSTDVEIKGYDEIDRFFDTVSESPKEMKAEVNKEEKLKEKVDSSFETFVEGISKELIGKGITVRKNLEVVRSSICKVKKGYTGDWVTAYLTITSTGYGHFFDISGLLNETEDLRNIVERLNGGVGKSLISIFDAGKHKKEPGEDRMVFEVNSKVGEMIETLSSPVISIYLNDNTYELDRERRQISASNRHQNGFASFFGVSEVKIKLFTLSNAIEIFHAFKDRNQPEESIDDFSESPEKVLDEGIGLKVLLQEENPWVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGY3</accession>
    <name>I6ZGY3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Guanylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_011040</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263945.1">
        <property type="nucleotide sequence ID" value="XM_009265670.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGY3"/>
    <dbReference type="GeneID" id="20520731"/>
    <dbReference type="KEGG" id="ero:EROM_011040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011040"/>
    <dbReference type="HOGENOM" id="CLU_001715_1_2_1"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="4">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="184" mass="21304" checksum="6313F1AFF505070E" modified="2012-10-03" version="1">MIDTVHLVFTGPSGSGKSTIISHILSKFPFKFSVSHTTRAPRKGEVNGKDYHFVSTKEFEEMVRNQEFLEYVQYNGNYYGTGVSQLKNSQKTVLLDLEYEGVLYCKKNYPNFVILYIHCDKNVARERLEKRMGNKSEKDIEARMSLYEAFNSIKDKCDYVIDNTHSLEKSKGEVEEIISKTVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGZ6</accession>
    <name>I6ZGZ6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein-S-isoprenylcysteine O-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.100</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_011190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12125"/>
            <dbReference type="Rhea" id="RHEA-COMP:12126"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90510"/>
            <dbReference type="ChEBI" id="CHEBI:90511"/>
            <dbReference type="EC" id="2.1.1.100"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="5"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263960.1">
        <property type="nucleotide sequence ID" value="XM_009265685.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGZ6"/>
    <dbReference type="GeneID" id="20520746"/>
    <dbReference type="KEGG" id="ero:EROM_011190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011190"/>
    <dbReference type="HOGENOM" id="CLU_120081_0_0_1"/>
    <dbReference type="OrthoDB" id="422086at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714">
        <property type="entry name" value="PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12714:SF9">
        <property type="entry name" value="PROTEIN-S-ISOPRENYLCYSTEINE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="22609" checksum="49398DC465ACC38B" modified="2012-10-03" version="1">MECPSFYFALGSLFMLGISHSSCYLALHIVSSTSLLIFLHSRERVRNIDFGMRIIRHDDFVILFGCLLLESLFVPKSAGYFGGRVLKAVAACGLVFSITFFFVTLRHMPDNKTGGRRIQRSGPYRFVRHPLYSSLILYWASCTIYLSCFVSLAIFLWFVSSRIIPRIKECEREMVSTSSEYMEYRKAVWSGIPMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHA4</accession>
    <name>I6ZHA4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIF4G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_021030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264075.1">
        <property type="nucleotide sequence ID" value="XM_009265800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHA4"/>
    <dbReference type="GeneID" id="20520865"/>
    <dbReference type="KEGG" id="ero:EROM_021030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021030"/>
    <dbReference type="HOGENOM" id="CLU_453420_0_0_1"/>
    <dbReference type="OrthoDB" id="2190870at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035145">
        <property type="term" value="C:exon-exon junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039762">
        <property type="entry name" value="Nmd2/UPF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 2 UP-FRAMESHIFT SUPPRESSOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12839:SF7">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="1">
        <location>
            <begin position="256"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="603" mass="69321" checksum="3A3AA8311C5FF037" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHL1</accession>
    <name>I6ZHL1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Synaptobrevin/VAMP-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030420</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264160.1">
        <property type="nucleotide sequence ID" value="XM_009265885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHL1"/>
    <dbReference type="GeneID" id="20520953"/>
    <dbReference type="KEGG" id="ero:EROM_030420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030420"/>
    <dbReference type="HOGENOM" id="CLU_074848_1_1_1"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="119"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="176" mass="20245" checksum="97F17C3563DDD542" modified="2012-10-03" version="1">MAIISLVVINVHTKKIKAKAFSLGQFSFFTRGKVKETLTFISQELAEKLETYEFQEYSHEFNDEKTYKLFSLVHNDYAYIACADDNYPGIVALKLLQEARHGNIEKLIDEYKDPASKHTLLQIQNEVEETKGALSKTLMAVLERNDKLEDLVAKSEHLCYETKLLFKSAKKKNKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJD2</accession>
    <name>I6ZJD2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FACT complex subunit POB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_070970</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SSRP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264845.1">
        <property type="nucleotide sequence ID" value="XM_009266570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJD2"/>
    <dbReference type="GeneID" id="20521657"/>
    <dbReference type="KEGG" id="ero:EROM_070970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070970"/>
    <dbReference type="HOGENOM" id="CLU_017374_0_1_1"/>
    <dbReference type="OrthoDB" id="2193092at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="11">
        <location>
            <begin position="303"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="394"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="402"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="413"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="424" mass="48593" checksum="DCCDB4DAF518E451" modified="2012-10-03" version="1">MEILTLSDCFLKSKTDKKATVKISRDGIAIKAEGGEVYTMEKNEVKDAELFHGSRKMAIRIFGNAVYEINNVDQNYIDELKRMFSEYFKISLYVKELEIADVLCGELGINGQKALEFRSTKTIFEVPVEDIESVVDIRNELSISLKDVEIRFVSNKKAIEEIKEGCSSSVDDEIFKMEGLSLAYPRGKFNLIFFRDYLRLVGSSYDHKVYYKSVRQLYILEKGYIRDEERYVVACVDPPIRQGQTRYDYIVASFDDAEGEMNANDERLKKEYSGLHSEIFTEVMETLCVIKAVRSMFESRDGMRCLRCAIKAYEGQLYPLEDCVLFLPKAIRLGLNEISLVEFSRINLSSMQAKTFDMTLFCEGSYTFNGLSKDEFGMLEQYFHSKGVKARSEIIDDGASSDSEEDDYESENDSMVVDSDSYDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJJ7</accession>
    <name>I6ZJJ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_071670</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264915.1">
        <property type="nucleotide sequence ID" value="XM_009266640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJJ7"/>
    <dbReference type="GeneID" id="20521731"/>
    <dbReference type="KEGG" id="ero:EROM_071670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071670"/>
    <dbReference type="HOGENOM" id="CLU_085878_2_0_1"/>
    <dbReference type="OrthoDB" id="1162399at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="RNA polymerase Rpb7-like N-terminal" evidence="6">
        <location>
            <begin position="10"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="76"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="17181" checksum="38FA12472D1F6B66" modified="2012-10-03" version="1">MFFVRDFVQNIHLSPSYLGPNIQTLIEEYLLSKVEGSCSSSGYVVMVLSIDEVSESRIILTGETIFTVKYKALTLKPFKGEIIDANVVETNKMGVFASVGPLTVFISNHQIPNFLLENEITKDIMIRLKIIGTKIDSTKIYAVGTLNDDSLGIIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJP5</accession>
    <name>I6ZJP5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264965.1">
        <property type="nucleotide sequence ID" value="XM_009266690.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJP5"/>
    <dbReference type="GeneID" id="20521782"/>
    <dbReference type="KEGG" id="ero:EROM_080460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080460"/>
    <dbReference type="HOGENOM" id="CLU_871627_0_0_1"/>
    <dbReference type="OrthoDB" id="2195567at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="228"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="319" mass="34504" checksum="EC3C6374A895F072" modified="2012-10-03" version="1">MLKVNSESIKRTLNIHVASAVLAFIGGILKLSSTFTEKQQVKDISKLGLRGILVLSCLGFLWNSVDTMLRRVTAERFKRDNGFLNSSVIVSLLEIIMMLVCIKMMFFYPLVANGEGQDAAAPRGLPEESKFGLFGAIFLPYIFSECLRLLLSYRKNMKGDKIILAIIITGCLVMGGLGIVDYKNKGNYIGGGIFAIGLLMVLARLGFMHEEDVPVLGEEDSDEEFDSTTKYVIIGATFLLVLAILLCSHKILFKDCTLIDYLKSFNLFGNAETNGAQNNTLANATKMSESQGDLDAPPDGGISARSGTSPGSGAEGTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJQ4</accession>
    <name>I6ZJQ4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA primase small subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_080580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264975.1">
        <property type="nucleotide sequence ID" value="XM_009266700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJQ4"/>
    <dbReference type="GeneID" id="20521794"/>
    <dbReference type="KEGG" id="ero:EROM_080580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080580"/>
    <dbReference type="HOGENOM" id="CLU_028288_3_2_1"/>
    <dbReference type="OrthoDB" id="19606at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0639">Primosome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="279" mass="32611" checksum="2523A907A5BF3DBA" modified="2012-10-03" version="1">MGLDQQLARTYYRTLFPIDLMFKCLRIDENREVSFGLASGGYIRFLTFRTADEFRKKLLGIVPERIDVGAIYRDRPTKHNAPKIVGKELVFDVDLTDYPRECCSKKLICNKCLVLIKVAVKILDYSLKQELGFKNVGFVFSGRRGVHCWVFDDTALELSDIERGEIVRYYDCVAKRKMYSEEYSKILREYSPFFESEGLEESVLFERMYIRIDKEVTQKSKHLIKMPFSVHPSTEIISVPIDPSDLDSVKLEDFPRLKDVLESPERLDPYLDVARSWRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJV7</accession>
    <name>I6ZJV7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coatomer subunit beta prime</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_081270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265040.1">
        <property type="nucleotide sequence ID" value="XM_009266765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJV7"/>
    <dbReference type="GeneID" id="20521862"/>
    <dbReference type="KEGG" id="ero:EROM_081270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081270"/>
    <dbReference type="HOGENOM" id="CLU_005507_1_0_1"/>
    <dbReference type="OrthoDB" id="10261470at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030126">
        <property type="term" value="C:COPI vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050844">
        <property type="entry name" value="Coatomer_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056176">
        <property type="entry name" value="TPR_COPA_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876">
        <property type="entry name" value="COATOMER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19876:SF2">
        <property type="entry name" value="COATOMER SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23953">
        <property type="entry name" value="TPR_COPA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="93"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="179"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="220"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="COPA/B TPR" evidence="4">
        <location>
            <begin position="583"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="721" mass="81364" checksum="0B40AE785E5EBD77" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK77</accession>
    <name>I6ZK77_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA repair and recombination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265155.1">
        <property type="nucleotide sequence ID" value="XM_009266880.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK77"/>
    <dbReference type="GeneID" id="20521980"/>
    <dbReference type="KEGG" id="ero:EROM_090410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090410"/>
    <dbReference type="HOGENOM" id="CLU_933925_0_0_1"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="72"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="298" mass="34341" checksum="791D854C5EBC2CFF" modified="2012-10-03" version="1">MKVLTGKANLLSGISMLRKVCNHPRLFFSRRPDVSEDYSSEVSNEKNNEGSRTSLDGEERYGLVSSSCKVKILMDLLKKWKKEGSKVLIFSQTIRMLDIIERCINKYTYLRMDGRTPTSIRSSLVDRFNRSEDVFIFLLTTKVGGLGLNLTGASRIVIYDPDWNPSTDTQAKERAWRYGQRKGVEIYRFICKDTIEEKVYQKQIFKDLLGKKVLSNPRLNRFFNKSCINELFSFTVTGGIDEVRVDEAHKCDLEGGALKEIRDRDSEVFSRIKSLNEKKVLSGSEVLEYIRLRERGCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKS6</accession>
    <name>I6ZKS6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ARF guanine nucleotide exchange factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_101030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265415.1">
        <property type="nucleotide sequence ID" value="XM_009267140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKS6"/>
    <dbReference type="GeneID" id="20564533"/>
    <dbReference type="KEGG" id="ero:EROM_101030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101030"/>
    <dbReference type="HOGENOM" id="CLU_287713_0_0_1"/>
    <dbReference type="OrthoDB" id="430364at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF388">
        <property type="entry name" value="GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="302"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1035"/>
            <end position="1063"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1063" mass="123921" checksum="70CBDED2896CB691" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL42</accession>
    <name>I6ZL42_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265540.1">
        <property type="nucleotide sequence ID" value="XM_009267265.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL42"/>
    <dbReference type="GeneID" id="20564660"/>
    <dbReference type="KEGG" id="ero:EROM_110610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110610"/>
    <dbReference type="HOGENOM" id="CLU_1906720_0_0_1"/>
    <dbReference type="OrthoDB" id="2194617at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="141" mass="15892" checksum="EC2D25A55FD88682" modified="2012-10-03" version="1">MLHMDAEFISGVVVSYSKLLKEMSGMRKEIKEVLKTVRDVLSHINTTCNPSLPSSSTSKSECLSNIQKPCLDKKTALMLPKEALENCVALVGRKENRYRIPISCKGRAKRSSLKACDSHPSFFEEIGREVYYAQLEDDLSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL61</accession>
    <name>I6ZL61_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal protein L7Ae-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265560.1">
        <property type="nucleotide sequence ID" value="XM_009267285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL61"/>
    <dbReference type="GeneID" id="20564680"/>
    <dbReference type="KEGG" id="ero:EROM_110810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110810"/>
    <dbReference type="HOGENOM" id="CLU_084513_3_0_1"/>
    <dbReference type="OrthoDB" id="5364946at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="109" mass="11984" checksum="D8AC5106DA240B74" modified="2012-10-03" version="1">MIPIASPLASDSVEEMIARLVTEQAERGTLSSGIKKCQKCVLEGSKGLLVLTADTTPMDLITHLPALCEDRGIKYIFVRKKSSIPNRFTCVFLEMDGSDTLNRILETLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL75</accession>
    <name>I6ZL75_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Superoxide dismutase</fullName>
            <ecNumber evidence="6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_110960</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 superoxide + 2 H(+) = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="6"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265575.1">
        <property type="nucleotide sequence ID" value="XM_009267300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL75"/>
    <dbReference type="SMR" id="I6ZL75"/>
    <dbReference type="GeneID" id="20564695"/>
    <dbReference type="KEGG" id="ero:EROM_110960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110960"/>
    <dbReference type="HOGENOM" id="CLU_031625_0_1_1"/>
    <dbReference type="OrthoDB" id="239262at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.55.40.20:FF:000004">
        <property type="entry name" value="Superoxide dismutase [Fe]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="entry name" value="Iron/manganese superoxide dismutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595">
        <property type="entry name" value="37S RIBOSOMAL PROTEIN S26, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43595:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="Fe,Mn superoxide dismutase (SOD), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Manganese/iron superoxide dismutase N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Manganese/iron superoxide dismutase C-terminal" evidence="8">
        <location>
            <begin position="103"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="226" mass="26335" checksum="1D46DAA7B931A16F" modified="2012-10-03" version="1">MNFALPNLDYPYDALEPFVDEETMRTHHTKHHQGYINSLERTIRNNGLEDQDLNFYVTKSRRIRGLKDNVRRDLINFSGGHYNHSLFWKMMCPPGTSEPISPKLLECIDKSFGSQEKMVEKFNGIAASLFGSGWVWLCYRRNEGVLVIRKTYNQDSICMKTSSTIPILGLDVWEHAYYLKYKNARAEYVANWWKVVNWGMVNKIFEEIALANKKLHVVSDGSIKFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL98</accession>
    <name>I6ZL98_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Forkhead/HNF3 transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111220</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265600.1">
        <property type="nucleotide sequence ID" value="XM_009267325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL98"/>
    <dbReference type="GeneID" id="20564721"/>
    <dbReference type="KEGG" id="ero:EROM_111220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111220"/>
    <dbReference type="HOGENOM" id="CLU_1085975_0_0_1"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000135">
        <property type="entry name" value="forkhead box protein G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045912">
        <property type="entry name" value="FOXJ2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46078:SF2">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46078">
        <property type="entry name" value="FORKHEAD BOX PROTEIN J2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="7">
        <location>
            <begin position="112"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="5">
        <location>
            <begin position="112"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="204"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="261" mass="30230" checksum="B468A0935AE2CA3A" modified="2012-10-03" version="1">MSCNMTSVELFDIEPSEESIDQDMRGLYKSIEEDPMASDVLCEFSGVGFDGDGAIHRFYTKWISSGMRPTSPDTCIDPRRLYDNCFEMKEKNGKMRIYEEKEYACRDTSKSKPSLSYAQIITRAIRTSKVGKLTLSEIYKWIEDSFEYYRYANPVWKNSIRHNLSLNKCFKKVPRDPGTRGKGGRWMLDYDFLSKEDFKRRRGAKHYDSGVMERSESSGDCSHDEREDGFRDVEMSSKGLIGQMKKFMASEINPSKNVNKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLD2</accession>
    <name>I6ZLD2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265620.1">
        <property type="nucleotide sequence ID" value="XM_009267345.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLD2"/>
    <dbReference type="GeneID" id="20564741"/>
    <dbReference type="KEGG" id="ero:EROM_111420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111420"/>
    <dbReference type="HOGENOM" id="CLU_897232_0_0_1"/>
    <dbReference type="OrthoDB" id="2192172at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="310" mass="35809" checksum="BFF9CF10036EA27F" modified="2012-10-03" version="1">MENDKTVVELLFILSLFSLSLTFFPGLIKDSVEEVEYFKGNRASRCFNVLGVPKDNSIEFYAMSGSIGNIDTKDIPNGSPITRAIHDLYKSKKKFTIYINPRMEIIHIYSQCVIPRYIFKIKEVPSMNQPSIEIIKRLDSLYLPDGPMDQKLIQTLVYLGLKGRLENKLFILYENILVIINEKKQMILFNMDLEHLFGMMLKETSSRKDEKENGLNRLLIAFKIESNILFFCDPRNDRREFGKKMTGNTLAKGTVKLFPSSKDIQIKSALRRDDYGKLCELVSDEERKEGMCVLEKVIIDPIKPDESSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS64</accession>
    <name>I6ZS64_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cdk activating kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263938.1">
        <property type="nucleotide sequence ID" value="XM_009265663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS64"/>
    <dbReference type="GeneID" id="20520724"/>
    <dbReference type="KEGG" id="ero:EROM_010970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010970"/>
    <dbReference type="HOGENOM" id="CLU_120586_0_0_1"/>
    <dbReference type="OrthoDB" id="4951845at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="195" mass="22178" checksum="28A0F2B1681A09FE" modified="2012-10-03" version="1">MESVLRNDIVEGNKTILHLSRFLCMPYKTRLVSQIIFQQAYPKLFKKESYNIVALVSVYLASKINETLIKLDTILEAMAKVTHMGSVPPRERIIAIECKVIELIEFQFNINPVQFFLLRIGKTLNVDVLKKLAVLDEVHGDGRVNLIKYFNGVYGPEMVALSVLDDGELKLFECFFGVSVSRALVEEIKSVILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS80</accession>
    <name>I6ZS80_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit TAF9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_011170</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263958.1">
        <property type="nucleotide sequence ID" value="XM_009265683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS80"/>
    <dbReference type="GeneID" id="20520744"/>
    <dbReference type="KEGG" id="ero:EROM_011170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011170"/>
    <dbReference type="HOGENOM" id="CLU_068315_3_1_1"/>
    <dbReference type="OrthoDB" id="341924at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="137" mass="15366" checksum="2455A35820161C83" modified="2012-10-03" version="1">MASGENLAPRDAKVISVILRSLGIEECEPKVIIQLLEFAYKYTTDVLEDALLFAKHADRVHIITNDVKLALQTKVGRHFVPPPPRQYLSDIAAMVNSKPLSIPDGENLIKVPPSSSALLNLDYEVLRKDSDKKRRIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSN9</accession>
    <name>I6ZSN9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264118.1">
        <property type="nucleotide sequence ID" value="XM_009265843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSN9"/>
    <dbReference type="GeneID" id="20520909"/>
    <dbReference type="KEGG" id="ero:EROM_030020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030020"/>
    <dbReference type="HOGENOM" id="CLU_706022_0_0_1"/>
    <dbReference type="OrthoDB" id="10388351at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="391" mass="44878" checksum="CCA7E501DC16F0D2" modified="2012-10-03" version="1">MIYVFTLLQILRASNELDGDFNMGLVSKSDIEEIKGSISGLLWSHPSTEFDETSITLTRPGFGVIIEVDPKGWVQELVNTLGEERALLKTREESFHMTMFQVCVEFFKDGYDPNTLPWNIFSVFDDYIKKKLDKMTSSVDVPSCFEIRSCGEVMILGRDYPSHVVARVELKTIKEEMDKIKEKLNVLGYVWIKHFAKALEEKGYGGRITKDMRGIGVEGKGWKFNIGYTASKDYRTHITLGILRCGDPEYEKLTLWANGCVGRKINDTFSDGMGREEINEKTEELNKANENICRKLLEKFELEDLSNYPKLEEQVRNAKEGKKLDVNREVIDEWRRYLISSKLSDIQEKAEKTKGEEVKIIKLKYSTINMSRIQNFEPYSNSFNPAGGSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSS6</accession>
    <name>I6ZSS6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">T complex protein 1 subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_030120</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264128.1">
        <property type="nucleotide sequence ID" value="XM_009265853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSS6"/>
    <dbReference type="GeneID" id="20520919"/>
    <dbReference type="KEGG" id="ero:EROM_030120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030120"/>
    <dbReference type="HOGENOM" id="CLU_008891_5_1_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="700" mass="76885" checksum="94BD3C97E38CF23E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT37</accession>
    <name>I6ZT37_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264278.1">
        <property type="nucleotide sequence ID" value="XM_009266003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT37"/>
    <dbReference type="GeneID" id="20521074"/>
    <dbReference type="KEGG" id="ero:EROM_040100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040100"/>
    <dbReference type="HOGENOM" id="CLU_164173_0_0_1"/>
    <dbReference type="OrthoDB" id="2195425at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="13804" checksum="FBD2E8D27913DD52" modified="2012-10-03" version="1">MIFIGDTFDFEFTTKTSNPIRCAFGKQFSILLFSDNSGIYKVSAHHLCFVVPVHYPSFVRSVLKPKDLPLRESVDRLFEFKSSKNRDRFALYVDSISNDHFQIIKELGPPKNIRENKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTA5</accession>
    <name>I6ZTA5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">T complex protein 1 theta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_040990</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264363.1">
        <property type="nucleotide sequence ID" value="XM_009266088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTA5"/>
    <dbReference type="GeneID" id="20521163"/>
    <dbReference type="KEGG" id="ero:EROM_040990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040990"/>
    <dbReference type="HOGENOM" id="CLU_980146_0_0_1"/>
    <dbReference type="OrthoDB" id="1748577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="284" mass="31795" checksum="08C2711536E28FE3" modified="2012-10-03" version="1">MDIGQTHLGGLISNAQQDEKVRYHIVGSKVRMTCNLVKSLYGRSHRTKLIVNGYGQVLLSSQPGVIYDNIKMNHPLVKLLQDYVKKMDVVGDGATFFVVLVSELIQETIDIIGKGMKPTYLSHMLREVHKEVEDLTKGLRVEHIIRFEDRESISKVLRGILKDSNLEKMVSEGISLTRSFNSENIRVCKVACGSVEDSYVVQGMVFNRSPEGEVKHVLKGKTSIYNCPLDISRTELKGTVLMRTASELLSFSKDENKRTKKAVESMDGDVVICSGKGIRYILTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTG9</accession>
    <name>I6ZTG9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glycerol-3-phosphate dehydrogenase [NAD(+)]</fullName>
            <ecNumber evidence="9">1.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_050230</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4 9">
            <text>sn-glycerol 3-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.8" evidence="9"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264453.1">
        <property type="nucleotide sequence ID" value="XM_009266178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTG9"/>
    <dbReference type="GeneID" id="20521255"/>
    <dbReference type="KEGG" id="ero:EROM_050230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050230"/>
    <dbReference type="HOGENOM" id="CLU_033449_2_5_1"/>
    <dbReference type="OrthoDB" id="10263760at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141152">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000004">
        <property type="entry name" value="Glycerol-3-phosphate dehydrogenase [NAD(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017751">
        <property type="entry name" value="G3P_DH_NAD-dep_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03376">
        <property type="entry name" value="glycerol3P_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11728:SF8">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE DEHYDROGENASE [NAD(+)]-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="2 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent N-terminal" evidence="10">
        <location>
            <begin position="4"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Glycerol-3-phosphate dehydrogenase NAD-dependent C-terminal" evidence="11">
        <location>
            <begin position="191"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="9"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="266"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000114-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="345" mass="38613" checksum="1892066F5BF61188" modified="2012-10-03" version="1">MSKKVSIIGNGNWGTAIGRLLANNTIESSIFEKDVRMWGCREEFEGRALNDIINSDNINPKYLPGIHLPENLKAVDDIRTLADSDVLVFALPHQYIEAIEPLKGLVKNNCIGVSLTKGFIDAEDGDIDLVSRSIHRILDINVSVMMGANIADQVARDMISEGTLGYTDEDAADIVYKLFNSYTYRVTKIKDVYGVEISGALKNVVSMAYGFAEGLGYCTNTKVAIFRNGFAEMRKFFKFFYPMATTESLFQSSGVGDLLVSCMSGRNFGCAKMMAEKRMSLKEAEGTMRFTKLQGPTTALIVYNYLKRQKRIDEFPLMSTVYRICYENEAYDAILECISFESIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTK9</accession>
    <name>I6ZTK9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pseudouridylate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264498.1">
        <property type="nucleotide sequence ID" value="XM_009266223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTK9"/>
    <dbReference type="GeneID" id="20521301"/>
    <dbReference type="KEGG" id="ero:EROM_050690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050690"/>
    <dbReference type="HOGENOM" id="CLU_016902_12_1_1"/>
    <dbReference type="OrthoDB" id="424794at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050188">
        <property type="entry name" value="RluA_PseudoU_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600">
        <property type="entry name" value="MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21600:SF40">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE RPUSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pseudouridine synthase RsuA/RluA-like" evidence="1">
        <location>
            <begin position="91"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="324" mass="37156" checksum="02011D258DFED8AB" modified="2012-10-03" version="1">MPKGEAEYFFTYRTNVKGRWCARPVIDVLTSEFRTRTRDYFLSALECGVITVNEEVVEPTRMLRVGDIIKHTVHIHEPSHPEIDVIKIEEDYIVVNKPPGIPCHPTGGYREYSITRALFDDKKVACINRLDMPVSGVLIIATKNYSRCLERIKDAVKIYVAKVCGVFPERVDVDKSIKCTEGKNRCVNDEGKACLTLFRRLEYKDGYSLVECRPVTGRTHQIRIHLQSIGFPVINDIMYGDGVPPPLPENGVCNADINGFEDNKKYEHVVKHCKGRGNRSFITKDHYICLHAWKYIYNGTEYVANLPEWCRGWDLASHETQKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTQ4</accession>
    <name>I6ZTQ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_051200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264548.1">
        <property type="nucleotide sequence ID" value="XM_009266273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTQ4"/>
    <dbReference type="GeneID" id="20521352"/>
    <dbReference type="KEGG" id="ero:EROM_051200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051200"/>
    <dbReference type="HOGENOM" id="CLU_1185003_0_0_1"/>
    <dbReference type="OrthoDB" id="10442416at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="234" mass="27343" checksum="F2CC9E38B1061805" modified="2012-10-03" version="1">MEFTSIPDFQELNNVGSQIVIHNHSALLMTYSLLVAIRSLLECLSYQDVAQFSLEDDIKSTFTNPALFDRKIMETIEKTETNIDELLKKTKKWVNSIGLTVYNNVELNEFQEFPDFRSKVYPYFIFSIHNPQCSDARMVWEKLYEIVIESRRSLNIYDNTGIWKELIKVKNEMLESKEIEYLTKRVFLSAPVLTPLRLMDGVEDILNGKYNEWIFPYLELCGLLSLLSTITSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU92</accession>
    <name>I6ZU92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264723.1">
        <property type="nucleotide sequence ID" value="XM_009266448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU92"/>
    <dbReference type="GeneID" id="20521532"/>
    <dbReference type="KEGG" id="ero:EROM_061350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061350"/>
    <dbReference type="HOGENOM" id="CLU_124533_0_0_1"/>
    <dbReference type="OrthoDB" id="2191211at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="188" mass="20594" checksum="FCD2D96B93453643" modified="2012-10-03" version="1">MDELISELRSTQAKEERQIGSTVNTNDLPISNATSKGLSNEVPPDPFVQENLEETPDSEEDTKDDCHELTSSQVPISFHIMKLDFDALAQVKKENQVEIGIDKIKCCKKYKIENVFAGIIKKLSVFGADTACLELVDESGSICGSCPLAVVDEFGLKVGTIVLLSKCSLWKINGNHLNIVGANIRKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUA7</accession>
    <name>I6ZUA7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264743.1">
        <property type="nucleotide sequence ID" value="XM_009266468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUA7"/>
    <dbReference type="GeneID" id="20521552"/>
    <dbReference type="KEGG" id="ero:EROM_061550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061550"/>
    <dbReference type="HOGENOM" id="CLU_025143_0_0_1"/>
    <dbReference type="OrthoDB" id="2189733at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="692" mass="79998" checksum="B0197DFAB08A131C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUB0</accession>
    <name>I6ZUB0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tetrahydrofolate synthase</fullName>
            <ecNumber evidence="3">6.3.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Folylpoly-gamma-glutamate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Tetrahydrofolylpolyglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_061610</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + L-glutamate + ATP = (6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10580"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="Rhea" id="RHEA-COMP:14740"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolylpolyglutamate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.17" evidence="3"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264748.1">
        <property type="nucleotide sequence ID" value="XM_009266473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUB0"/>
    <dbReference type="GeneID" id="20521558"/>
    <dbReference type="KEGG" id="ero:EROM_061610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061610"/>
    <dbReference type="HOGENOM" id="CLU_015869_0_0_1"/>
    <dbReference type="OrthoDB" id="5212574at2759"/>
    <dbReference type="UniPathway" id="UPA00850"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="entry name" value="Mur ligase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136:SF5">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="FOLYLPOLYGLUTAMATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="MurD-like peptide ligases, peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01012">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="419" mass="46842" checksum="B951D835E9CB7D7E" modified="2012-10-03" version="1">MDVNQSKDYEDTIEKLKGLQSSICSLKAQIKEGKGGGKEYARRRMDRLRKLLGLLGITNRHIGDLNVVHVAGTKGKGSTCAMVDSILRNYGIRTGLYTSPHLIDPRERIRVGGRLIPHSKFAQYLNEVYYKIQEAESANGMPFFFEFMTILAFYVFVHEKVNGAIIEVGLGGLYDCTNIIRNPVVVGITSLGFDHIEVLGNTIEEIAKAGIFKPGVQAFTTHDNPISAQNVLKSYAKQIGCVLSICPPISSYTGGEKKICLGLDGEVQGCNASLALQLSHFYLTNRRKQIPDITKYPPLLTLCSETKKGPLPFLVSDKGLESLCKTYWPGRFQMIFHNGVHLYLDGAHTAESIFLCANWFKSRSKLKKVDKHKCFRALLYYCKGPRDCRDHVKILSKLDFDVVMLSPGAVHISDIWEPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUN0</accession>
    <name>I6ZUN0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator complex subunit 15 KIX domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_071650</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AFN83416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266638.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUN0"/>
    <dbReference type="GeneID" id="20521729"/>
    <dbReference type="KEGG" id="ero:EROM_071650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071650"/>
    <dbReference type="HOGENOM" id="CLU_356392_0_0_1"/>
    <dbReference type="OrthoDB" id="2189774at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.20">
        <property type="entry name" value="Coactivator CBP, KIX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036529">
        <property type="entry name" value="KIX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036546">
        <property type="entry name" value="MED15_KIX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16987">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="510"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="552"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="787" mass="89812" checksum="5CBA069472A71DA3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUZ5</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
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            <taxon>Microsporidia</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="20521890"/>
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    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081550"/>
    <dbReference type="HOGENOM" id="CLU_2277459_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="110" mass="11200" checksum="CB75A6B41E7910E6" modified="2012-10-03" version="1">MAESVNENNTSGDSNGNNRTKRNTIITIVVVVLVVTLLLAVASKKGWFGGNKGGSSTKVGGDPASKPPSLKSSEESGKGGNKGNSMKGSPSSNKEEENEVGGSNTSNLYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV26</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265098.1">
        <property type="nucleotide sequence ID" value="XM_009266823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV26"/>
    <dbReference type="GeneID" id="20521921"/>
    <dbReference type="KEGG" id="ero:EROM_081860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081860"/>
    <dbReference type="HOGENOM" id="CLU_312889_0_0_1"/>
    <dbReference type="OrthoDB" id="2190283at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031541">
        <property type="entry name" value="HTH_micro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17007">
        <property type="entry name" value="HTH_micro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="781" mass="91659" checksum="EE806E8B80FDB1FB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV69</accession>
    <name>I6ZV69_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="14">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">EROM_090080</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="14"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265123.1">
        <property type="nucleotide sequence ID" value="XM_009266848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV69"/>
    <dbReference type="GeneID" id="20521947"/>
    <dbReference type="KEGG" id="ero:EROM_090080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090080"/>
    <dbReference type="HOGENOM" id="CLU_518765_0_0_1"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16499">
        <property type="entry name" value="RING-HC_Bre1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 14 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 14" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="474"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="41"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="151"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="292"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="377"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="AFN83626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="526" mass="61116" checksum="651252907316CDFE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVN2</accession>
    <name>I6ZVN2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nucleotide excision repair factor TFIIH/TFIIKsubunit cyclin H-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265298.1">
        <property type="nucleotide sequence ID" value="XM_009267023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVN2"/>
    <dbReference type="GeneID" id="20564410"/>
    <dbReference type="KEGG" id="ero:EROM_091860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091860"/>
    <dbReference type="HOGENOM" id="CLU_109008_0_0_1"/>
    <dbReference type="OrthoDB" id="2196255at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="180" mass="20532" checksum="95BD6F25DE12AE55" modified="2012-10-03" version="1">MLRDEKEMKDVVYAIVILASKCEEIHGNIDTLIKKLPESNKESILNYESDMFEALDYNFHFPSLYLRMYGILAILQEKGIIRVAAETEGAVKVAEGSDDEGAFIYDGEECIVPCINKLWASSVRRMDKVLLLDKESYKEIELVYAGLHLPYEIFNSLDFPFNQDNVTKLRNACGAFQFLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVN6</accession>
    <name>I6ZVN6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_091920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265303.1">
        <property type="nucleotide sequence ID" value="XM_009267028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVN6"/>
    <dbReference type="GeneID" id="20564416"/>
    <dbReference type="KEGG" id="ero:EROM_091920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091920"/>
    <dbReference type="HOGENOM" id="CLU_663973_0_0_1"/>
    <dbReference type="OrthoDB" id="2190888at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707438" evidence="3">
        <location>
            <begin position="18"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="61"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="248"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="413" mass="47413" checksum="7553BF691CFAD5AD" modified="2012-10-03" version="1">MMIMVLLQLCVSKIVVVDDLGVTDRQNHDPDMARKVMNVLGGAIKNSKDTVLLSNSKGTKRIVEDDEKRPSKYSGSNHDELKSFSKEKNDESKKDENGGHSMSGPSKPEYTGRPGQNLKDENVIPEIGRSYQEDSLLNPLHIRSLFNGNNLEALGKNMYEGSNMQNYEPKDILMGYRSDQNKYTPFSFGGGDSFADVPRYGGTSLEDGMGFGMVGNNGPSDEIRARRSFIILKMAQSKQKESMLQSKIDKLLQDIDQIAEKIQDTQKMKDGMTSRLHTKKNHLRNLQANIKEVEVQRGRTLALIRLSRNELLKLSKMMDDQRSKLALLEDEEKIFSDRIKKYDEEYDIGNRELQLDETRSQEIKRNIEELERLYNVLKIRNNELLNERNKESAIRNRLELETERLDRNTIDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVR3</accession>
    <name>I6ZVR3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="15">1-acyl-SN-glycerol-3-phosphate acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_100190</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265333.1">
        <property type="nucleotide sequence ID" value="XM_009267058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVR3"/>
    <dbReference type="GeneID" id="20564449"/>
    <dbReference type="KEGG" id="ero:EROM_100190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100190"/>
    <dbReference type="HOGENOM" id="CLU_031891_0_0_1"/>
    <dbReference type="OrthoDB" id="202234at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF2">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE 4, ISOFORM D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="14">
        <location>
            <begin position="124"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="451" mass="52642" checksum="415B159A162E17EC" modified="2012-10-03" version="1">MKETILRRRTFLHDAIDMVSMGSHTLENDDFTRCFQPTSAMEMDSSGIFYTFSFVIRYFVLFPIRMAFLAICMLIFLLMILRAVLTKKSEHLENALMFGAKSLMLAMNARVNHIGEKKRHDGPHVYVSNHTSFVDFFLLSSHEFPHACVSERHGGLFGLLFKSILIRNGSIAFKRSEKVDRQLVVEKVKEHVRSGGAPMVIFPEGTCVNNKFSVLFQKGAFELGVTIYPVAIRFRRRLFDPYWNRRSHGFAMHVFYLMTRWRLEAEVVWMKPVSIMKDESPTQFSHRVKTMISKEAGLKNTLWNGFLKSSPAIKDREILRESYLITYERVVSNTLDRINSLDIEQNRFYLYDSSIDFSSLRSHSYFESMIPYKKFLNEVLKEYLRLKELPQKELKFLLANHKREEGSLRDSKMETFCSCKPRKNQKINSLRRRGNPLMSCKYESCTSHGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACY9</accession>
    <name>I7ACY9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_011160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263957.1">
        <property type="nucleotide sequence ID" value="XM_009265682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACY9"/>
    <dbReference type="GeneID" id="20520743"/>
    <dbReference type="KEGG" id="ero:EROM_011160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011160"/>
    <dbReference type="HOGENOM" id="CLU_066256_0_0_1"/>
    <dbReference type="OrthoDB" id="446723at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="76"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="272" mass="30990" checksum="2DAE3C48FFD2488B" modified="2012-10-03" version="1">MIPSLKRMVLNISDSKRNFGDHQKRLDHNIYIATKDSKKIGAYLLEPEGDRIPEGFIVALHGKGCSREDYVSSSKVDILVSKGYCVLIPDYRGFADSEGEFDVSMVNLDVMACFEYLISRFNAEEVHMVGHSLGTGIMAEYCGYIEKNSLGTRYRPQKMFMISAFTSITDVAMESVACRIISFLFPWIKEKLSKEMGYDTLKNMEHVNGEDIFLYHGMKDDIIPYHHSIMLSKKYGAKVRLSDHGHGNILRDEELWNDIHLNISGSSYKSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD02</accession>
    <name>I7AD02_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_011310</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0329 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263972.1">
        <property type="nucleotide sequence ID" value="XM_009265697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD02"/>
    <dbReference type="GeneID" id="20520758"/>
    <dbReference type="KEGG" id="ero:EROM_011310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011310"/>
    <dbReference type="HOGENOM" id="CLU_653872_0_0_1"/>
    <dbReference type="OrthoDB" id="2200041at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022115">
        <property type="entry name" value="DUF3654"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12376">
        <property type="entry name" value="DUF3654"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707386" evidence="3">
        <location>
            <begin position="19"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="345"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="368"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="420" mass="49545" checksum="45D2957FCCC48420" modified="2012-10-03" version="1">MIFIMRMWGIFLYSYVLCSSSSEERGAIGGVDHSSSVLYRRERIKKALEKAAAFDTKILIPMIFHNDKVVVTPVSEYHSIEKSEIEYAEDVINRIPCSAWNTMVCVFPPNNSNWIMNLVEQFFVEKRGRLSGVEMYKREKTKRKPKFTDLMMKIYQDNCDLLEGFGREVVKNVCSKIKSLKEDRNMNEKEKKEEMMVLEDIRKYGESLQTRWKKDEILKAQEIVCEVFKYFWEREEDKRIITTIMYLRVLSYGGGKIKKEVFPLIHYINHSVLVKTYNRYGIRAAGMLIKQVLIKDNDCNEEYAEKIGKEVQKMESEERRMEEEKKRMESKESGCEFEVLEGLEGGSSKRKGGERRRKGRRGVRKKEKRSERRMMEEACSGREGATSSKEPCDKQGSVHFYNIHKRVLRWKKDPKKIRKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD17</accession>
    <name>I7AD17_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA replication factor C small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_020200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263992.1">
        <property type="nucleotide sequence ID" value="XM_009265717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD17"/>
    <dbReference type="GeneID" id="20520778"/>
    <dbReference type="KEGG" id="ero:EROM_020200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020200"/>
    <dbReference type="HOGENOM" id="CLU_042324_2_0_1"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000952">
        <property type="entry name" value="Replication factor C subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="35"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="308" mass="34695" checksum="CA198023741942A4" modified="2012-10-03" version="1">MHQPWTEKYRPGTLLEMVGNKEVIASLQSILEAGKIPNMLFYGPPGTGKTTSIRAIARSLPRSCVLELNASDERGIATVRETIKDFASTYSKMMKLVILDEADMMSRDAQNALRRIIEDFSANTRFCLIANHSRKIIPPILSRCTKFRFGPIESTESRIKEICRKEGIKYTEDGIAAVSKVSEGDMRKAVNDVQGISLSLGLIDERNVHKFNGIAPAQIYEEVFMDLGNMSMVELRARIEDLRYEYGIDCDALIDNLGGIARKSKMRNKTKILKELSDIERRRSIGCNEKLQLDAVISVFILNRDNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD22</accession>
    <name>I7AD22_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">20S proteasome subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_020250</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263997.1">
        <property type="nucleotide sequence ID" value="XM_009265722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD22"/>
    <dbReference type="GeneID" id="20520783"/>
    <dbReference type="KEGG" id="ero:EROM_020250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020250"/>
    <dbReference type="HOGENOM" id="CLU_035750_10_0_1"/>
    <dbReference type="OrthoDB" id="204949at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03759">
        <property type="entry name" value="proteasome_beta_type_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033811">
        <property type="entry name" value="Proteasome_beta_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF10">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="206" mass="22774" checksum="B54781FB1D01C557" modified="2012-10-03" version="1">MSADIETFYGGSILAMASENAVAIMSDKRLGMGPITVSKDFVRIHRMGPRLYVGLSTFVPDTQVLLRRIRKNYNLFKLEEQREMEPEELSNMISFLLYSKRTSPYITAPIVAGLSSDGRPYISGMDCIGSITSPKDFVATGTAAKNLMGICEALYRPNMDEENLFVTCAQAFLNAVDRDALSGWGADCYVISEGKVIKRSIKGRCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADC0</accession>
    <name>I7ADC0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_021200</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264092.1">
        <property type="nucleotide sequence ID" value="XM_009265817.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADC0"/>
    <dbReference type="GeneID" id="20520882"/>
    <dbReference type="KEGG" id="ero:EROM_021200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021200"/>
    <dbReference type="HOGENOM" id="CLU_615425_0_0_1"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="446" mass="50445" checksum="1521631A66AFB813" modified="2012-10-03" version="1">MSADDLRTWLKAAAENKITTKNTWRSTLIDHFTNIDEFREKQGINFQKASCTLDGCAKVYSTRVDDVSENAVRLLEGFGREESKKKQSRKQTKTTIEKNIFNLNIKVNASRKSVDPKFLYLTSLPENVLMIDAVGISSDGVLRMSSSKDGKVIIIDDHLKLPISFPKGLLISPSISEDKEIEKMIIEPMADNDQEDSREFAGLDTDRNFDLSEIPLINDNQNFGYKEASVSHFKGWAGPNHWKIQAKKGRNGSQKPKETSFLDFTEVIDHESITEPGNTLFDPSFIVERRENRHILPQDFRLDVEDLYKYLIRDGSFYGKDQRCEDEAIDYSPSSCVMEEPEDEFIADIPLQGIQKNPIPFRKTSKKVNIKKLKDNVFDSVMMGNTTLLSIFESVPKIYGDDESKDISVHLCFISLLHLANDNNIQLKGVGNDVEVCILSKPGASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADC5</accession>
    <name>I7ADC5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SUMO ubiquitin activating enzyme E1 subunit UBA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_021250</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="4">Protein modification.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264097.1">
        <property type="nucleotide sequence ID" value="XM_009265822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADC5"/>
    <dbReference type="GeneID" id="20520887"/>
    <dbReference type="KEGG" id="ero:EROM_021250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021250"/>
    <dbReference type="HOGENOM" id="CLU_013325_7_0_1"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.520">
        <property type="entry name" value="Ubiquitin activating enzymes (Uba3). Chain: B, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023318">
        <property type="entry name" value="Ub_act_enz_dom_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF5">
        <property type="entry name" value="SUMO-ACTIVATING ENZYME SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="5">
        <location>
            <begin position="4"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="420" mass="48827" checksum="5AF61A7B5540A8E1" modified="2012-10-03" version="1">MINGRVLVIGCGGIGCELLKLLAKQNLRSITLIDCDTIDLSNLNRQFLFNRDDIGKNKAVVAARTFKKLNKKCRVLPICADITEFDAMFFARYKVVYSCLDNAEARSYVNQRCLISNTPLVDGGCGGFKGQAYYFDYNSECFDCIPRKFSKEYLMCTIRSRPTRFEHCIIWAKYVLLEMRLKVDENSQDFYQRFLKDVIENCEDMSTADKLEKFRNSEDYKERTKKIVEILGKLDSILFNKDNRDILEYIYNAAYIRGRCAGIEPISFDEAVTVAGNIIPSLSTINSIVASLMMLSVRNKCNYYSVDNGNVIRRLETCERNPECSTCSHHWYVISYDGPLIFRRLIRCFQKHSLELVAYSDKRLFLTCDMTEYLDKDLEFENNSIGEVICMENNKRVRIYLYFLRRGGNLSFRRIYKAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADL2</accession>
    <name>I7ADL2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264147.1">
        <property type="nucleotide sequence ID" value="XM_009265872.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADL2"/>
    <dbReference type="GeneID" id="20520940"/>
    <dbReference type="KEGG" id="ero:EROM_030290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030290"/>
    <dbReference type="HOGENOM" id="CLU_771675_0_0_1"/>
    <dbReference type="OrthoDB" id="2194086at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="359" mass="42117" checksum="C07B2A90790C5700" modified="2012-10-03" version="1">MPMAAEGIKASFLGQEVFVTVDDTRVRIKRDPSTVAIQNSENDLVIRDIPLIEIRSLHVKNNEGFFLKLKLSEETIVLRFKTLHSRDIIKSIILSFMKTDQDITKKILESDPAMRVLFNNLKQWVSPSKFWSINRDKMKQMVAIVRQQPSKEMEIDEKSFISSLDPVLLNIFEQMNCSINQFYNLLRQSYFCDPKNEKNSLDRMVSSAVRSHEVKQDFASRINTHSVLALKPIEDVKIDPSTKEGKKVEFLPIYPFEEEKTERPRRRFVFEKKPLRCEVELEITPMVEKKVFEKKDLAWIRDLSKIVYKSMKEKDGEFLKEVAGITKRFLDGMEKKYGKGCDVYLRRILPTYFIEKKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADL9</accession>
    <name>I7ADL9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_030390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the V-ATPase C subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264157.1">
        <property type="nucleotide sequence ID" value="XM_009265882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADL9"/>
    <dbReference type="GeneID" id="20520950"/>
    <dbReference type="KEGG" id="ero:EROM_030390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030390"/>
    <dbReference type="HOGENOM" id="CLU_785333_0_0_1"/>
    <dbReference type="OrthoDB" id="6605928at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.10">
        <property type="entry name" value="subunit c (vma5p) of the yeast v-atpase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1180">
        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036132">
        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10137:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03223">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118203">
        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="353" mass="41149" checksum="81AD0449F22A4D17" modified="2012-10-03" version="1">MFILVGLPVEEDTPHPTEGQIQEEYGVHSSRILLPSFTGVSLEGIVNQVERLNSLEKSCEGLLKTFVGYSGGDNMGKGNVCNSAERMKEFLKWDSQSFMTNSIDKVILLLEEEYKRINKAYEEKAEEFNEAKKECDRLQKLTRGNLCDINLNIIVEKQEKYEFLKVMYVVVQKSKSSEFNRIVYESPHISSDAVEKINSDEDHDLFKLYILHHSEEEIRNMMHEEGFLIKDLDENMISSKEMITERRKAEERFSAIERILMTFVHIHLVEALKILVHVKLLRLFVESVYRYGLPTEYMFFVTNGEKSKILSQWITIAKDWPSDRIVYEEEGDNNEEGEIFFAFSEIEVHGEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADZ2</accession>
    <name>I7ADZ2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">40S ribosomal protein S11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_040570</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264322.1">
        <property type="nucleotide sequence ID" value="XM_009266047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADZ2"/>
    <dbReference type="GeneID" id="20521121"/>
    <dbReference type="KEGG" id="ero:EROM_040570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040570"/>
    <dbReference type="HOGENOM" id="CLU_073626_0_2_1"/>
    <dbReference type="OrthoDB" id="10254436at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000008">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0164">Citrullination</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 16" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="15">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN82825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="156" mass="17841" checksum="7DCC3430E89FFF07" modified="2012-10-03" version="1">MEETKKLKVYPRQTGVYAKPYASEDEKPARKVREEVCGFKAPETAYTRQYIDKKCPFTGDVNVRGRIFKGVVKKMKAEKTIVVVVNYVHYSKKYKRYSRRHTNFSVHMSPCFEGMINVGDVVMCGETRPLSKTKSSVVLGYVKKALPGSFKLLESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE97</accession>
    <name>I7AE97_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Actin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264467.1">
        <property type="nucleotide sequence ID" value="XM_009266192.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE97"/>
    <dbReference type="GeneID" id="20521269"/>
    <dbReference type="KEGG" id="ero:EROM_050370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050370"/>
    <dbReference type="HOGENOM" id="CLU_036505_0_0_1"/>
    <dbReference type="OrthoDB" id="7340501at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="554" mass="64006" checksum="5DEAF3A5427B3B88" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEG2</accession>
    <name>I7AEG2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_051240</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264552.1">
        <property type="nucleotide sequence ID" value="XM_009266277.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEG2"/>
    <dbReference type="GeneID" id="20521356"/>
    <dbReference type="KEGG" id="ero:EROM_051240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051240"/>
    <dbReference type="HOGENOM" id="CLU_1194879_0_0_1"/>
    <dbReference type="OrthoDB" id="2190817at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="232" mass="27394" checksum="7BD13CACD2331AB8" modified="2012-10-03" version="1">MTEESRLKVVLKCISYIQEPKFLILLHLLEMAWGAAGLAYLDDLSFFIINKDLMNSIKGEDTPEFSQQIVSLLDSARDRLIDIEEEVGMWYIDGHKLAEVELGTDAMMEFYQYVGREFKEIFEESDHAEMPPRGGNEKALWERLFQIIDACKAEEMNQNQNISEWIERFKKEFFDSECVKFITTRSIVYSSHTFEKQKRAVRRVLLEGRDKDQLFDFHELPRCFRLISRLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFM8</accession>
    <name>I7AFM8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Developmentally regulated GTP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_081290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265042.1">
        <property type="nucleotide sequence ID" value="XM_009266767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFM8"/>
    <dbReference type="GeneID" id="20521864"/>
    <dbReference type="KEGG" id="ero:EROM_081290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081290"/>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_1"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000003">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="62"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="286"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="362" mass="40707" checksum="8288AE7FC6D4AE68" modified="2012-10-03" version="1">MGIQDKINEIEAEMARTQKNKKTEHHLGILKAKLARYRHELDAPKAKASRSEGFEVSKSGDARVALIGFPSVGKSTLLSKITNTYSRAAEHEFTTLECISGKMYLNGTWIQVLDLPGIVSGASQNRGRGRQVISIARTADLILMVLDPRRHEDKRTLENELHEMGIRLNKKKPDISLTCTTSGGINIGVTCQLTKTTEETIKAILREYKINNCQMFVREDVCDDDIIDLVSGSSVYVDCLYVYNKIDELSLEDFNKVAEYPDSTLISCQKEWNIDMLLEDIWNKLGLTRIYTKKKGVFPTLDDPVVIREGGTVKDLCAHIHKDFLLSFKHALVWGTSAKHSPQKVGLGHTLEDEDVVQIFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFR1</accession>
    <name>I7AFR1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bromodomain-containing transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_081640</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265077.1">
        <property type="nucleotide sequence ID" value="XM_009266802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFR1"/>
    <dbReference type="GeneID" id="20521899"/>
    <dbReference type="KEGG" id="ero:EROM_081640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081640"/>
    <dbReference type="HOGENOM" id="CLU_001499_5_1_1"/>
    <dbReference type="OrthoDB" id="784962at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050935">
        <property type="entry name" value="Bromo_chromatin_reader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880:SF225">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN BET-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22880">
        <property type="entry name" value="FALZ-RELATED BROMODOMAIN-CONTAINING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51525">
        <property type="entry name" value="NET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="4">
        <location>
            <begin position="168"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="5">
        <location>
            <begin position="288"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="120"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="370" mass="41939" checksum="9CEC5651028702CC" modified="2012-10-03" version="1">MESDEASAGSILAEHQLKYCSQVLTRIKRNSNAPPFLEPVDPVKLGIPDYPEKIKNPMDLSTIRKKLDAKEYSGPEGFDDDMKLMFNNCYTYNPPGTIVHDMGKALESAYNDMMAGMPQEVSKKRKKSEIPVTGRSKQVKRNAKPVEGMKAEDYEFCSEVLSDLVKPKHKSYNWPFLEPVDGDLVPGYYSVIKEPMDIQTMKNKLDQRKYHSVEEFGRDLELIVENCKKFNAPGTEVYACGQEFEKAVKAHMEKTSPVDIKNKISELKRKVMSYTREIRMLESKLVEQTGEAPSSRAYSLSERVSIGNAILNMTKEQTENVAKIVLKNGAGEFVENDEIEVDMRTIPDHVVEEIDMYIKSINIGGDVQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFY5</accession>
    <name>I7AFY5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(6)-L-threonylcarbamoyladenine synthase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N6-L-threonylcarbamoyladenine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_090120</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>L-threonylcarbamoyladenylate + adenosine(37) in tRNA = N(6)-L-threonylcarbamoyladenosine(37) in tRNA + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="9">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265127.1">
        <property type="nucleotide sequence ID" value="XM_009266852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFY5"/>
    <dbReference type="GeneID" id="20521951"/>
    <dbReference type="KEGG" id="ero:EROM_090120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090120"/>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_1"/>
    <dbReference type="OrthoDB" id="10254073at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:tRNA N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd24132">
        <property type="entry name" value="ASKHA_NBD_OSGEP_like_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000038">
        <property type="entry name" value="Probable tRNA N6-adenosine threonylcarbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034680">
        <property type="entry name" value="Kae1_archaea_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03722">
        <property type="entry name" value="arch_KAE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF14">
        <property type="entry name" value="TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Gcp-like" evidence="10">
        <location>
            <begin position="20"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="126"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00030439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="328" mass="36176" checksum="E3ACEA8890F0C7F9" modified="2012-10-03" version="1">MGLEGSANKLGIGIMKDNTILANERFTYAPPPGEGFIPAKTAEHHRSKILDLIAISLEKAAICLSDVDVFCYTKGPGMGLPLAVVATVARTLSLYCNKPLIPVNHCIAHIEMGRFMTKAENPVVLYASGGNTQIIAYHNKRYRIFGETLDIAVGNCIDRFARALRLPNFPAPGLSVERYAKLGKNYIELPYVVKGMDVSFSGILSNIKSKIVENDQMKYDLCYSLQETIFSALVEVTERAMAFSNSKEVLIVGGVGCNLRLQEMMSIMAKERGGISYGMDERFCIDNGLMIAYAGMLMAKSGSSFNLDECFVTQRYRTDSVEVAWRDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG96</accession>
    <name>I7AG96_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Nucleolar GTP-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091640</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Nucleus</location>
            <location evidence="2 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class YlqF/YawG GTPase family. NOG2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265277.1">
        <property type="nucleotide sequence ID" value="XM_009267002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG96"/>
    <dbReference type="GeneID" id="20564388"/>
    <dbReference type="KEGG" id="ero:EROM_091640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091640"/>
    <dbReference type="HOGENOM" id="CLU_011106_4_0_1"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01858">
        <property type="entry name" value="NGP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000559">
        <property type="entry name" value="Nuclear/nucleolar GTPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024929">
        <property type="entry name" value="GNL2_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012971">
        <property type="entry name" value="NOG2_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF9">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08153">
        <property type="entry name" value="NGP1NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="8">
        <location>
            <begin position="153"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="410" mass="46855" checksum="AF7E1EB4C8564E51" modified="2012-10-03" version="1">MRENYYNKKKLKYLEMLRSGRPKKNSRGDIIKDAPFQSSKADVGRIEPSRKWFSGSKTISPTELDAFRSETKIESPYNVLLSTGKVPYSLLEDGTKKRKAKDFGAVFGKKSVRKKPNLQYNSLEELSQQVKEVKERKKEIKKEHVKGQSHRIWLELYKVLDSSDVIIHVLDARDPLGTMCDKIANYIKEEAPHKHLMYVLNKVDLIPTGVTAKWLRYFSKSHPTIAYHSNSITKNYGKANLIGLLKQLSKLYKKRHLSVGFVGYPNTGKSSIINTLRNKEVCKVAPVPGETKVWQYITLTGGIYLIDCPGIVPIPNYDQAVLRGAVRIENIEDPEDYIDMIIEKARNSIAKAYRIQFVDSADLLERLAVRFGKLRKGGEPNTNAVSKMILHDWVRGKIPYFVPPKEDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGM6</accession>
    <name>I7AGM6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">1-acyl-SN-glycerol-3-phosphate</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_101150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265427.1">
        <property type="nucleotide sequence ID" value="XM_009267152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGM6"/>
    <dbReference type="GeneID" id="20564545"/>
    <dbReference type="KEGG" id="ero:EROM_101150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101150"/>
    <dbReference type="HOGENOM" id="CLU_041302_0_0_1"/>
    <dbReference type="OrthoDB" id="189226at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07990">
        <property type="entry name" value="LPLAT_LCLAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983">
        <property type="entry name" value="1-ACYLGLYCEROL-3-PHOSPHATE ACYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10983:SF16">
        <property type="entry name" value="LYSOCARDIOLIPIN ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="2">
        <location>
            <begin position="86"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="357" mass="41935" checksum="411DE310BF41851E" modified="2012-10-03" version="1">MAEVMRRVWNGMQFIIAMIVYFSYIIVGLIVVSVAKLVCCGEKSQRVLRKSAKVGWLHLTRAMFSRYFPQRVFIRYNPIILKRNRNVVISNHLTEYDWLFVCCVLHYLKRFEDLCIILKMSLRNIPLLGYGMKFFQFIFLNRKVSKDREIIMSGASRLKKEGKYDLLLFPEGTYIDKCSHPKSHKWSVEANVEVEGKGFDPEEVLIPRTTGFKILRENIRDDMEGVIDLTMIGNPYVKYPNDVFSYWDVIINKSQKVNFMFFLDYIPNNDGMDGDDFLLKLFEEKEKMISRFRKENGDRCIRSIDEFKNVADSLSRPETEHIDMEIELSSHWGPFFYVMFILVSGLLLGSAIRFCSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGZ8</accession>
    <name>I7AGZ8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Probable isoleucine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="3">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_110980</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265577.1">
        <property type="nucleotide sequence ID" value="XM_009267302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGZ8"/>
    <dbReference type="GeneID" id="20564697"/>
    <dbReference type="KEGG" id="ero:EROM_110980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110980"/>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_1"/>
    <dbReference type="OrthoDB" id="1706657at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000023">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="14">
        <location>
            <begin position="15"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="15">
        <location>
            <begin position="688"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN84080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1018" mass="118418" checksum="9EA67384ACC13487" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH22</accession>
    <name>I7AH22_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Eukaryotic translation initiation factor 4E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265602.1">
        <property type="nucleotide sequence ID" value="XM_009267327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH22"/>
    <dbReference type="GeneID" id="20564723"/>
    <dbReference type="KEGG" id="ero:EROM_111240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111240"/>
    <dbReference type="HOGENOM" id="CLU_043552_7_0_1"/>
    <dbReference type="OrthoDB" id="590761at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF18">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E HOMOLOGOUS PROTEIN, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20620" checksum="D3D0789C0E124E53" modified="2012-10-03" version="1">MNKLPSRFHVTLKVLEGSENGVSKDTYEKDLKSICTLESQEHLAYLLHHLKEFDKIDKQFYINVFKEGISPVWEDKNNMNGCDWSLMLKKEVCQRYFERLLISLCSGYFKTFEPTGIVGIHKDNRFKLSIWSRGLPPTTDCGEIISELKAALQIDFVITFHYKNHSKILAYHKSLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH31</accession>
    <name>I7AH31_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein transport Sec13-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_111340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265612.1">
        <property type="nucleotide sequence ID" value="XM_009267337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH31"/>
    <dbReference type="GeneID" id="20564733"/>
    <dbReference type="KEGG" id="ero:EROM_111340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111340"/>
    <dbReference type="HOGENOM" id="CLU_032441_0_2_1"/>
    <dbReference type="OrthoDB" id="364224at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090114">
        <property type="term" value="P:COPII-coated vesicle budding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037363">
        <property type="entry name" value="Sec13/Seh1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SEC13 / SEH1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11024:SF2">
        <property type="entry name" value="PROTEIN SEC13 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="9" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN84115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="288" mass="32002" checksum="B8BB299600FE07E2" modified="2012-10-03" version="1">MLPKYLLFVFRNMEAPMDIHKDAIHSIETDFSQKRILTSCSDGMVRIFSKGTAESPHDLALELELNGNSGPATKAIFLNQGEFIASSYFNGKVIIWKYEGGRFNKKYEKQLLNGSVNDIAGRWDGDSFTLFCGCSDGNVRILTVDSSFSATESEVFCHRFGVSSVSAMENGFVSGGMDYSVAVWEGNEEVARFRDHKGFVRNVAVCPTNSFKLLCIATCSEDGSVIIYTRKGSEYKSQKIILEEPCYSLSWSFSGFSLSVGYGSSKFKCFVPDSNGEFKEVKLKKVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7AHA5</accession>
    <name>I7AHA5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Structural maintenance of chromosomes protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111890</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. SMC5 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265667.1">
        <property type="nucleotide sequence ID" value="XM_009267392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AHA5"/>
    <dbReference type="GeneID" id="20564788"/>
    <dbReference type="KEGG" id="ero:EROM_111890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111890"/>
    <dbReference type="HOGENOM" id="CLU_004969_2_1_1"/>
    <dbReference type="OrthoDB" id="10254973at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030915">
        <property type="term" value="C:Smc5-Smc6 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45916:SF1">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecF/RecN/SMC N-terminal" evidence="5">
        <location>
            <begin position="12"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="156"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="244"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="319"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="725"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1025" mass="120026" checksum="099AD42DEEC1CDDF" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL33</accession>
    <name>I7AL33_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">BRX1 RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010140</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRX1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263856.1">
        <property type="nucleotide sequence ID" value="XM_009265581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL33"/>
    <dbReference type="GeneID" id="20520640"/>
    <dbReference type="KEGG" id="ero:EROM_010140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010140"/>
    <dbReference type="HOGENOM" id="CLU_048373_2_0_1"/>
    <dbReference type="OrthoDB" id="1638493at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026532">
        <property type="entry name" value="BRX1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13634:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BRX1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="5">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="23203" checksum="77EAF068A3581205" modified="2012-10-03" version="1">MTSVILSTRGATAKIRHLMKDISKLVKVEEEQKWDMGKDYRELKNLIEMNECDSMLFFRSTKRSDDLWIGILNGMSVLFRMHNVFTMKDCNFPVNSFRDCGYVLMFSKEFDEIEHLRCAKNVIDHVFQSNEIKDKALCFFYLDGFIWVRCYKIGKKLEEIGPRFVLEVLKVFEKCFEGKVLYKVEKRDCPVEKDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL38</accession>
    <name>I7AL38_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263861.1">
        <property type="nucleotide sequence ID" value="XM_009265586.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL38"/>
    <dbReference type="GeneID" id="20520646"/>
    <dbReference type="KEGG" id="ero:EROM_010190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010190"/>
    <dbReference type="HOGENOM" id="CLU_407697_0_0_1"/>
    <dbReference type="OrthoDB" id="2196090at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="556"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="343"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="608"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="556"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="582"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="668" mass="77703" checksum="D2D910918690172B" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL57</accession>
    <name>I7AL57_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_010390</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263881.1">
        <property type="nucleotide sequence ID" value="XM_009265606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL57"/>
    <dbReference type="GeneID" id="20520666"/>
    <dbReference type="KEGG" id="ero:EROM_010390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010390"/>
    <dbReference type="HOGENOM" id="CLU_038421_0_1_1"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07032">
        <property type="entry name" value="RNAP_I_II_AC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033901">
        <property type="entry name" value="RNAPI/III_AC40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF13">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="7">
        <location>
            <begin position="41"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="305" mass="35324" checksum="68835B7BAE257FA7" modified="2012-10-03" version="1">MSKIYKLERETMVDLHCSEVKSFEQTLDDIEIKITEYSGERLEFDLVNVDCSFANALRRILCNDVPTMAVKDVFLKENESVFPDEFIAHRLGLIPLEADPEMFEFVEKELNERNSLRFVLNVTNESKDVMNVYSDDIKWIPFGGQREALRNAKFCSGVLMFKLARGQKINAELVCTKNIGRVHAKWSPVCPATYRLMPLIEIGDVFDEDAYKLQKCFSEGVIDVRLSDGRMKAYVVNPRLDTMSREALRHEEFKDKVFIGRRSDYFLFTINSVFLDPLYLLKKAVSILIEKSMALRNEVEDLLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALK9</accession>
    <name>I7ALK9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pre-mRNA 3'-end processing cleavage and polyadenylation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_020840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264056.1">
        <property type="nucleotide sequence ID" value="XM_009265781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALK9"/>
    <dbReference type="GeneID" id="20520845"/>
    <dbReference type="KEGG" id="ero:EROM_020840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020840"/>
    <dbReference type="HOGENOM" id="CLU_043168_0_0_1"/>
    <dbReference type="OrthoDB" id="26282at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045243">
        <property type="entry name" value="Rna14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008847">
        <property type="entry name" value="Suf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980:SF0">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19980">
        <property type="entry name" value="RNA CLEAVAGE STIMULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05843">
        <property type="entry name" value="Suf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Suppressor of forked" evidence="4">
        <location>
            <begin position="27"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="493" mass="57872" checksum="6BDB8320E2E1179B" modified="2012-10-03" version="1">MDVEENAGIELESPSAVMEHARDLYMSKDYRNLEVLFGKCLKKSYNLDFWMLYIEYVKKVSQKKFKLYEVYEFTLGQFENYWDSYVLYKEYIEELGKIEDEQTRIEKIRNGYMRALQTPMNSLSELWKDFENFELELNKITGKKIVSDTLPIFQSSFQRYQQIQPLIRSWSIKNAAKLINLEMENEAKLGARAHESRMHFIHSYILDSFYYADEAYFFYSEYLIGIGQKERAKKVIQRGIGMNDGMFLSLYYGFVMDEESVYADLKGKYCAKEGDGTEKTLSREGDLIRINHLNYVLKKRGLESFRKLFIELGGEGIGPHVFIYCAFVEYYATGSRATPYNIFSSGLLKHPTSTLLKEEFFLFLLKIGDEENARALFKRLEKTSRMWDSMIEYEFMVGSMELFRELVDQKMGSIRDNTILPSLPVKDQSIQMEGILGKYHCFLSSFNFLDLKIKDNSRLLDEFVENLPKISQQNNVLSNLKPEKIIDLLKCIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALW0</accession>
    <name>I7ALW0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose/Na cotransporter-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264131.1">
        <property type="nucleotide sequence ID" value="XM_009265856.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALW0"/>
    <dbReference type="GeneID" id="20520922"/>
    <dbReference type="KEGG" id="ero:EROM_030150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030150"/>
    <dbReference type="HOGENOM" id="CLU_090086_0_0_1"/>
    <dbReference type="OrthoDB" id="2189463at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022057">
        <property type="entry name" value="Chs7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35329:SF1">
        <property type="entry name" value="CHITIN SYNTHASE EXPORT CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12271">
        <property type="entry name" value="Chs7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="270" mass="30496" checksum="148A2723DDBFD31D" modified="2012-10-03" version="1">MHPIDKVCSVSNFPMCEHLSDEICKDVSRGSLLFKTKVSSPTTVILLVMVMLSAVRMIRHVKALCSSVGRKEMLVFFNLYFGSAALEAVLVGFKESISKQTHLFLTSIQLSLYTSGFFSLFVGSITVDMFTGILGFRAATLLKISTAIYSVGIGTVIFTGLTAPAKEIASIPFFVLNPLFFFLYMVFQIKRLRKTDGEVWAYGTLVISTTCFLIANTVLFVGSRMIGLMTDRYFDNLFFYHFFILCTFIMIHKYWLSVYNYEVESLQLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMB1</accession>
    <name>I7AMB1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264331.1">
        <property type="nucleotide sequence ID" value="XM_009266056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMB1"/>
    <dbReference type="GeneID" id="20521130"/>
    <dbReference type="KEGG" id="ero:EROM_040660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040660"/>
    <dbReference type="HOGENOM" id="CLU_1602699_0_0_1"/>
    <dbReference type="OrthoDB" id="2193363at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="167" mass="19672" checksum="EFD4AE0A10C7BD8B" modified="2012-10-03" version="1">MYIPCITVMAFFVYQVLEIGMSDMIEHIFVNPAVHKIHNFPGILKMEYNPNDPWVNFYAFKSGVMCTPILLLPLMVKLILLALTFKRSDKKDNNAFLWVHMILMLFLTFADMIVLYTYDQDKTKNLSPNLNIYIYRNHTWFYLTHCIAEFISLGWTVGMCYGLLFCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMQ2</accession>
    <name>I7AMQ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ser/Thr protein phosphatase PP2-A regulatory subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264511.1">
        <property type="nucleotide sequence ID" value="XM_009266236.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMQ2"/>
    <dbReference type="GeneID" id="20521314"/>
    <dbReference type="KEGG" id="ero:EROM_050820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050820"/>
    <dbReference type="HOGENOM" id="CLU_060773_0_0_1"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000009">
        <property type="entry name" value="PP2A_PR55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11871">
        <property type="entry name" value="PROTEIN PHOSPHATASE PP2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037309">
        <property type="entry name" value="PP2A_PR55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="367" mass="41598" checksum="BAC5B0EABB5377FE" modified="2012-10-03" version="1">MGNNRIPMWKQCRAFTLEPNSLDNKEEATSVLYGENLYVGCGDGRVNCYGDKKLYSFQGFELEFDYLESCDVAEKVLAIERFDDGGLNELLVVGNERNLKIWKVRNKGPTKEVLEGRHSEGYECVCIKECRNVHPCILNSLSLNNDRQYLLSSDYLKINLWKPEKIDGCFTIVDVKPHKYSDLMFVINSTKFSKEMNMVFGYSTSSGEIHVNDLRLSSKSLEALTIDGVDADGIEGAVKSISDFQFVDSNLILARSLNSVTLYDMRNPKDNIFTTVLCDDVDEISSVYESDAVYARFRVCCSDNYGFTGGFRNTVHIINLLDGSKEDLPIQCKPKNTEKKDRLKLVSSRGNDFIIACEGKILEYRHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANH1</accession>
    <name>I7ANH1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264816.1">
        <property type="nucleotide sequence ID" value="XM_009266541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANH1"/>
    <dbReference type="GeneID" id="20521627"/>
    <dbReference type="KEGG" id="ero:EROM_070680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070680"/>
    <dbReference type="HOGENOM" id="CLU_842058_0_0_1"/>
    <dbReference type="OrthoDB" id="2191570at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="330" mass="37606" checksum="E694ED8C74F86788" modified="2012-10-03" version="1">MEIEYKSMACSIIVTLGLMVGLSMESFVCKTANCAFFLSSTLFISIVLTLFNLAHMMRAKTRITPISAYTGSISALIDIMIIFVYTKILHMNSLFFCLAYLMYQVTASILSCFIPFGSSAFMICICVIFLSPLFDLWFVEEHGFSRSQVPDFMRYYITNRSMNIIFFIPIVGIRVLFGEFFGRIGDRESSNFFSFLAMAVAGTMLAFVDTSTFKEELHKMDSVFIPFIVSNSILAGSLIIAKLNRSINLPSVYSPMSVLFIIISSLFCSSAMPWVWKKTILEHSLNSPYLFYLFTFSLFILYIVIVRKDSIVSRPTRDGFAPRMTWWLWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANR3</accession>
    <name>I7ANR3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264921.1">
        <property type="nucleotide sequence ID" value="XM_009266646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANR3"/>
    <dbReference type="GeneID" id="20521737"/>
    <dbReference type="KEGG" id="ero:EROM_080010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080010"/>
    <dbReference type="HOGENOM" id="CLU_1408746_0_0_1"/>
    <dbReference type="OrthoDB" id="2192163at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="199" mass="23287" checksum="20753173598A64BC" modified="2012-10-03" version="1">MGKLFKKFRIEKANERKKAKEKPKIKKTQEIRSHTKERSETEKIFCCLDVSYRRKTGYRKILVLDEVELERNIFLILEKVLEVVVTFDIVDSLFYSVLKRIVYLKNSVSIHTLLIDYFLGSILCGVNYEKFGCFLLKHLPQLLSSSKGRILPIIPNGELKESISKLKPGSKFLTFKYQQKFMVLKDGVLTTTNKYQFGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP12</accession>
    <name>I7AP12_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265036.1">
        <property type="nucleotide sequence ID" value="XM_009266761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP12"/>
    <dbReference type="GeneID" id="20521858"/>
    <dbReference type="KEGG" id="ero:EROM_081230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081230"/>
    <dbReference type="HOGENOM" id="CLU_177947_0_0_1"/>
    <dbReference type="OrthoDB" id="2186985at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031492">
        <property type="entry name" value="DUF5101"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17031">
        <property type="entry name" value="DUF5101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="103" mass="11948" checksum="A19C76C0D5584266" modified="2012-10-03" version="1">MFMRPVLNETTNATKRSDSKGKEIESDKERVLCFLKNTYMKTRDHSLRYDLKKCLEIIEGKENQEITDLRNALEEALVENEALFAEKCELAVTLECMKAEKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP45</accession>
    <name>I7AP45_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265076.1">
        <property type="nucleotide sequence ID" value="XM_009266801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP45"/>
    <dbReference type="GeneID" id="20521898"/>
    <dbReference type="KEGG" id="ero:EROM_081630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081630"/>
    <dbReference type="HOGENOM" id="CLU_891440_0_0_1"/>
    <dbReference type="OrthoDB" id="20729at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="235"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="200"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="312" mass="35168" checksum="841F9EF918EB4FEB" modified="2012-10-03" version="1">MKNVCRFFIEGKCKFGSKCKYDHVRYTSLVDPPQWIYSSYRGLDVMEISPEEVRFALMCGDMKEVLDNFWIRNYVALCAEMDLLESDARIASVSNRYVDPRENPDVFMAPFDVDRVLSTIKQMREQGARTLQKGHGDGQRGDGNWAGGFSGPPHQYPVNNRGGYKDKGNNWNRQETNPGNSWSQGNNKSAGTGYGGHKNGYNEKEPRGFSKESPYSKGKDNEYIQRNTWGYSRSTGRKYSKPDGEFDIQDVPSSFSGSYGGVAPKGTGGTWMHNGETLGDRQGIGRGSGDEEFIEEDFEYGKVPYSYRKPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7APK6</accession>
    <name>I7APK6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Mevalonate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_091780</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="9">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265291.1">
        <property type="nucleotide sequence ID" value="XM_009267016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APK6"/>
    <dbReference type="GeneID" id="20564402"/>
    <dbReference type="KEGG" id="ero:EROM_091780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091780"/>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_1"/>
    <dbReference type="OrthoDB" id="1652964at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="10">
        <location>
            <begin position="22"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="11">
        <location>
            <begin position="158"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="240" mass="26600" checksum="385B0CF1D2D74959" modified="2012-10-03" version="1">MKSSKKSETIVIDAEGNHINLIEKYKHVVPGFSKILSLEAPLGCGLGTSAAISLLLSYGKTRNTDILKEALEIENVFHGKASGVDVMACYTGGLISFKKGTVQKLPAHHLSRFKILIFNAKIPKNTEIAIRLGKRKKELYDEIGKVSEEAYKLLQREFTLQELYKLIRRNQDLLDELGVCPEEMKDVVDKMREIGVEAKITGAGCGGHLVTVVKKEQEIPGWRSVSIDLEGFHAFGTGFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7APY0</accession>
    <name>I7APY0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_101190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265431.1">
        <property type="nucleotide sequence ID" value="XM_009267156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APY0"/>
    <dbReference type="GeneID" id="20564549"/>
    <dbReference type="KEGG" id="ero:EROM_101190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101190"/>
    <dbReference type="HOGENOM" id="CLU_114645_2_1_1"/>
    <dbReference type="OrthoDB" id="2967263at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="12058" checksum="3EF35A9FA8597742" modified="2012-10-03" version="1">MVNIPKTRNTFCKKCSNYSTHKVSQAKKSKDNPRAQGNRRYARKQRGYGGQTKPILRRKAKVTKKLVLKLECTKCKFAHQKPLKRAKHVIFGGEKKRRGEALIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7APZ5</accession>
    <name>I7APZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263879.1">
        <property type="nucleotide sequence ID" value="XM_009265604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APZ5"/>
    <dbReference type="GeneID" id="20520664"/>
    <dbReference type="KEGG" id="ero:EROM_010370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010370"/>
    <dbReference type="HOGENOM" id="CLU_116395_0_0_1"/>
    <dbReference type="OrthoDB" id="2196169at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="202" mass="23751" checksum="C2EC9B777A3C5147" modified="2012-10-03" version="1">MIIRMVSTPNILGAEVISDVQRAYTIKLEKRYVLKFTNGEVTLKAFKELFLLDKEILFYVVNFEFFLYKMSLFKHYRIEFVTYPDGVKDERIERYKSVEKGISGEWSSRGVDLSFTPDVFASISSSLLSPELYLSELNLSGVIYKTLVKKLEYKNLKRQAREVIETHMNAEDEFDEEFDKHLKSLVFTGVVKMKDGLFYRWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ08</accession>
    <name>I7AQ08_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leptin receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263894.1">
        <property type="nucleotide sequence ID" value="XM_009265619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ08"/>
    <dbReference type="GeneID" id="20520679"/>
    <dbReference type="KEGG" id="ero:EROM_010530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010530"/>
    <dbReference type="HOGENOM" id="CLU_178976_0_0_1"/>
    <dbReference type="OrthoDB" id="2192716at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0675">Receptor</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="102" mass="12063" checksum="7CE0231D5972BD54" modified="2012-10-03" version="1">MQAEDYNDNHQRGIHWGFTKVLMVAVLYGLSLVCIILGLKPLFDMEFEIKSFANLAFVAFHGFYMFSFMAVHKKSHFIFWSISYLILSGISLFFYFYEDLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ53</accession>
    <name>I7AQ53_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chromosome segregation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_010980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263939.1">
        <property type="nucleotide sequence ID" value="XM_009265664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ53"/>
    <dbReference type="GeneID" id="20520725"/>
    <dbReference type="KEGG" id="ero:EROM_010980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010980"/>
    <dbReference type="HOGENOM" id="CLU_001042_9_0_1"/>
    <dbReference type="OrthoDB" id="10255539at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43977">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="3">
        <location>
            <begin position="419"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="254"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="571"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="793"/>
            <end position="853"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN82442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1001" mass="116376" checksum="0ACFA1630698E7F3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ73</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_011180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263959.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ73"/>
    <dbReference type="GeneID" id="20520745"/>
    <dbReference type="KEGG" id="ero:EROM_011180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011180"/>
    <dbReference type="HOGENOM" id="CLU_758696_0_0_1"/>
    <dbReference type="OrthoDB" id="2194817at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707779" evidence="1">
        <location>
            <begin position="18"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="365" mass="42089" checksum="A020646B8E9F982B" modified="2012-10-03" version="1">MGLLRLFFNALVAHVSRDKMSECEPADAPSGDMAIPTNLNREILYESFISINSLVFICEMNQFTDELMEEFVKKTVFVAKELLVENPSAFNQFNNYLTKVDGVGRDLKDALELRNKYFNNDRSMIGRELSAQKYKELRSRANGRLPRLYYEDKDEEYLQMMKRLMSAKHGDDGIHKAINREMAEFYGEKTEISKTLVLDSRISLSIGGSIFAPGIFANLIRSLSIPNISEIINRAVPRLIAPCEDLISKMHLKSSTERRSLVLDFLYGCLKRKNGPFKEKIDKAFEERTKILDIYLKGDPKAQKKLQILDKFQEDEINKLLFVFKNIKDDDLALRIDRAVVEAYIDFLENNQVSAKENLYGLFMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQA9</accession>
    <name>I7AQA9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265561.1">
        <property type="nucleotide sequence ID" value="XM_009267286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQA9"/>
    <dbReference type="GeneID" id="20564681"/>
    <dbReference type="KEGG" id="ero:EROM_110820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110820"/>
    <dbReference type="HOGENOM" id="CLU_921425_0_0_1"/>
    <dbReference type="OrthoDB" id="2190237at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="302" mass="35477" checksum="009FF8F64CF1CD87" modified="2012-10-03" version="1">MVVKNFMTTKEKDYVAESLRRNMRYFRSDIDYAGLFRQGQEKIEVKYDDEEPKRSINLVFGKENKKSSRRPRPYCGMDAQSTMAQVRSMLLLEELIDLYTVRRYCKLSEDDLKENIRILRSSLLSKFDIFISITKFLRFLGEYFSYIEEDERKEIIYLLQKTFHLVKDVPEFYNSMGLLLNSIPLVSESDLEDGFVSTFLFFPSGLILATSLLIRNEALCDVFYEAIINEPSWPFTKNKHGWQFLSVMISMVSEEKQRSIVKKARSYLLEIVKGPNNDEMDGAILFLETIGVEVDEFRCWSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQC2</accession>
    <name>I7AQC2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Nucleoporin autopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_020370</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264009.1">
        <property type="nucleotide sequence ID" value="XM_009265734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQC2"/>
    <dbReference type="GeneID" id="20520795"/>
    <dbReference type="KEGG" id="ero:EROM_020370"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020370"/>
    <dbReference type="HOGENOM" id="CLU_417429_0_0_1"/>
    <dbReference type="OrthoDB" id="3797628at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="7" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase S59" evidence="10">
        <location>
            <begin position="471"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="177"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="262"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="299"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="299"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="615" mass="65957" checksum="8AA84F2BE698D049" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQF4</accession>
    <name>I7AQF4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polyadenylation factor I complex subunit Fip1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_111280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FIP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265606.1">
        <property type="nucleotide sequence ID" value="XM_009267331.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQF4"/>
    <dbReference type="GeneID" id="20564727"/>
    <dbReference type="KEGG" id="ero:EROM_111280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111280"/>
    <dbReference type="HOGENOM" id="CLU_1468148_0_0_1"/>
    <dbReference type="OrthoDB" id="1917198at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007854">
        <property type="entry name" value="Fip1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051187">
        <property type="entry name" value="Pre-mRNA_3'-end_processing_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484">
        <property type="entry name" value="FIP1-LIKE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13484:SF0">
        <property type="entry name" value="PRE-MRNA 3'-END-PROCESSING FACTOR FIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05182">
        <property type="entry name" value="Fip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pre-mRNA polyadenylation factor Fip1" evidence="6">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="88"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="147"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="185" mass="21649" checksum="12F5E887EAF48100" modified="2012-10-03" version="1">MQNDEEFFIGDRDDESTSSDLELIVDKIPGKEEQKEEAEGQNILDYDIESFTDKPWNKPGADITDYFNYGFNETTWREYCNMQRRKSEFARGGAEWTDEKYGGVRRGFNEIKREGEGDGSRSRRGNRAEREGGRRGSAEGQRWEGWNGRKDGGRRRGDREERGQGRYEERGEGRRYGYRDGSKRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQW5</accession>
    <name>I7AQW5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ubiquitin-activating enzyme E1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_030810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ubiquitin-activating E1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264199.1">
        <property type="nucleotide sequence ID" value="XM_009265924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQW5"/>
    <dbReference type="GeneID" id="20520992"/>
    <dbReference type="KEGG" id="ero:EROM_030810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030810"/>
    <dbReference type="HOGENOM" id="CLU_002556_0_0_1"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031510">
        <property type="term" value="C:SUMO activating enzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019948">
        <property type="term" value="F:SUMO activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.80:FF:000001">
        <property type="entry name" value="ubiquitin-like modifier-activating enzyme 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.180">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, FCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.80">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, inactive adenylation domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2660">
        <property type="entry name" value="Ubiquitin-activating enzyme E1, SCCH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042302">
        <property type="entry name" value="E1_FCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018965">
        <property type="entry name" value="Ub-activating_enz_E1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042449">
        <property type="entry name" value="Ub-E1_IAD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019572">
        <property type="entry name" value="UBA_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042063">
        <property type="entry name" value="Ubi_acti_E1_SCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033127">
        <property type="entry name" value="UBQ-activ_enz_E1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000011">
        <property type="entry name" value="UBQ/SUMO-activ_enz_E1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF4">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1 C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10585">
        <property type="entry name" value="UBA_E1_SCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01849">
        <property type="entry name" value="UBIQUITINACT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00985">
        <property type="entry name" value="UBA_e1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00865">
        <property type="entry name" value="UBIQUITIN_ACTIVAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-activating enzyme E1 C-terminal" evidence="8">
        <location>
            <begin position="867"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="7">
        <location>
            <position position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="989" mass="112044" checksum="9712A30651EAB1F0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR08</accession>
    <name>I7AR08_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264269.1">
        <property type="nucleotide sequence ID" value="XM_009265994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR08"/>
    <dbReference type="GeneID" id="20521064"/>
    <dbReference type="KEGG" id="ero:EROM_031500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031500"/>
    <dbReference type="HOGENOM" id="CLU_1602698_0_0_1"/>
    <dbReference type="OrthoDB" id="2193576at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.20">
        <property type="entry name" value="Apolipoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372">
        <property type="entry name" value="DAUER UP-REGULATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47372:SF5">
        <property type="entry name" value="LATE EMBRYOGENESIS ABUNDANT PROTEIN (LEA) FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="169" mass="18723" checksum="77A24EEE213D626F" modified="2012-10-03" version="1">MGISKESCDHSSSSQCRECSNDKSYERKGSEMKDKTKNTTSNAMGKTKEKTNETMGRTGDAANETKEQTKNKASELGDKTKDTANDTMGKTKEKTNETKEQAKNKASSMGDKTKEKANNVKSSIDNKDKNEVYENIKEGGNKAWNKTKEVASSMKDGLKDLTNNNEQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR59</accession>
    <name>I7AR59_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Seryl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_040690</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR59"/>
    <dbReference type="GeneID" id="20521133"/>
    <dbReference type="KEGG" id="ero:EROM_040690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040690"/>
    <dbReference type="HOGENOM" id="CLU_023797_0_1_1"/>
    <dbReference type="OrthoDB" id="10264585at2759"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
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        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Serine-tRNA synthetase, tRNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
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        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11778">
        <property type="entry name" value="SERYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="tRNA-binding arm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="11">
        <location>
            <begin position="177"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="47"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="265"/>
            <end position="267"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
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        <location>
            <position position="265"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="281"/>
            <end position="284"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="352"/>
            <end position="355"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="387"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for serine binding" evidence="8">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="PIRSR" id="PIRSR001529-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="429" mass="49292" checksum="68EFE0B4BA8090A3" modified="2012-10-03" version="1">MIDINLIRDPKTREEVVESEKKRFRDGTAVGKAYELDRKRIETAFKLDQINARVNQLNREVKSGYKQGKKKEDEELAKKIDEIKNLYDEAKNLKEDSGRIEEELNKVMKGIGNIISEEVVVSNDEKDNLIVRSYRSGRSIQRNPQPFSMLMKDFTHSTAGAKVIGHRGYYLSGKMARLAQALTRYAIDFLENKGYTYIQTPVMLRRDVMAKTSQLSDFDDQLYKVEDDLYLIATSEQALAALYMDERMIPQEVPKKLCGQSLCFRKEAGAHGKDNAGLFRVHQFEKIEQFVICEPSESRKHHEEMIRVCEEFYQSLDISYNVVSIVSGELNDAAAIKYDLEAYFPNADKYRELVSCSNCTDYQSRELEIRYGVVKENNRKVYVHLLNGTMCAIQRALCCIVENYQKENGVGIPDVLQGYFGADFIELSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR73</accession>
    <name>I7AR73_ENCRO</name>
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        <submittedName>
            <fullName evidence="1">Endoplasmic reticulum translocation complex subunit Sec66</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266079.1"/>
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    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040900"/>
    <dbReference type="HOGENOM" id="CLU_133988_0_0_1"/>
    <dbReference type="OrthoDB" id="2189913at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031207">
        <property type="term" value="C:Sec62/Sec63 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018624">
        <property type="entry name" value="Sec66"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28229:SF1">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09802">
        <property type="entry name" value="Sec66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="19445" checksum="1A14B6697AA7B426" modified="2012-10-03" version="1">MILLLAMILSTMILMFSFIKRINRRKTPIEENPNTELEMYFLQKSEGAPSSVLLKQLVKAAACFKERVMKLEKDMEILGTLYEDRMISEEHWERINEESKNCEVERICIESEANIIKPGYGEEIFKDASKISITNTKKGKSSGDNNALYQKKKEALEFELMRKLGVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR84</accession>
    <name>I7AR84_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">DNA topoisomerase</fullName>
            <ecNumber evidence="3 11">5.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_041050</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="3 11"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264369.1">
        <property type="nucleotide sequence ID" value="XM_009266094.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR84"/>
    <dbReference type="GeneID" id="20521169"/>
    <dbReference type="KEGG" id="ero:EROM_041050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041050"/>
    <dbReference type="HOGENOM" id="CLU_002929_1_1_1"/>
    <dbReference type="OrthoDB" id="430051at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031422">
        <property type="term" value="C:RecQ family helicase-topoisomerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.290.10:FF:000001">
        <property type="entry name" value="DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="entry name" value="Topoisomerase I, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390:SF21">
        <property type="entry name" value="DNA TOPOISOMERASE 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PROKARYOTIC DNA TOPOISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Toprim" evidence="13">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="15">
        <location>
            <begin position="55"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="14">
        <location>
            <begin position="534"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="domain" description="GRF-type" evidence="14">
        <location>
            <begin position="584"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="492"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="492"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN82872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="622" mass="71566" checksum="16CFE3AB054FD74A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARI6</accession>
    <name>I7ARI6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PI3/PI4 protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264484.1">
        <property type="nucleotide sequence ID" value="XM_009266209.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521286"/>
    <dbReference type="KEGG" id="ero:EROM_050540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050540"/>
    <dbReference type="HOGENOM" id="CLU_224908_0_0_1"/>
    <dbReference type="OrthoDB" id="5570127at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF1">
        <property type="entry name" value="TRANSFORMATION_TRANSCRIPTION DOMAIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="1">
        <location>
            <begin position="2258"/>
            <end position="2807"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="3441" mass="396766" checksum="56E7F5B3C043D96B" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARK5</accession>
    <name>I7ARK5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264504.1">
        <property type="nucleotide sequence ID" value="XM_009266229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARK5"/>
    <dbReference type="GeneID" id="20521307"/>
    <dbReference type="KEGG" id="ero:EROM_050750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050750"/>
    <dbReference type="HOGENOM" id="CLU_061615_0_0_1"/>
    <dbReference type="OrthoDB" id="10261640at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031544">
        <property type="entry name" value="WD40-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17005">
        <property type="entry name" value="WD40_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="34226" checksum="1940BE14C7123534" modified="2012-10-03" version="1">MDSEVYGIDLNARRNILIHGGGDEKCTVTDIETGDVISIVEDFSDSVIFCKFIDDERFIVSSLDGTISLMTLEGEINNVCVNEDISQIKINGDRLLVGTVGGSVHLFTLDLCIQNVCIGQCNEIQDVCYENGKVYTLSKTNFVIFDALSHQKLMDVHVRGGSAMDKIPLSDVVCLGLEGCIVVRKNSHLLSKISIEGSPECILYMNNYFIVGGDFEDILLINIPMGMRTFKIPTGTERILSIKGDGENKIVFSTCSGVVGYGDIRDERSFKLIEGGVGCIFDLCFKDKMVYVGGEHGFNALDLGRVEELTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARN2</accession>
    <name>I7ARN2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA sliding clamp PCNA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_051060</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processivity during elongation of the leading strand.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264534.1">
        <property type="nucleotide sequence ID" value="XM_009266259.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARN2"/>
    <dbReference type="GeneID" id="20521338"/>
    <dbReference type="KEGG" id="ero:EROM_051060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051060"/>
    <dbReference type="HOGENOM" id="CLU_043978_3_0_1"/>
    <dbReference type="OrthoDB" id="534348at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043626">
        <property type="term" value="C:PCNA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019985">
        <property type="term" value="P:translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="5">
        <location>
            <begin position="16"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="6">
        <location>
            <begin position="140"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="29800" checksum="F338C452F569CF9F" modified="2012-10-03" version="1">MFELELTAREKDKDGAQIDSLSLMKRVLESLNEIVETADIKVTEKGLSIQVMDVTHVAFADIFLSSAMFTKYRCDRELVIGVQLKTFTKIIKGMSVEGGGVFRLECDDATTDLNVKNIREGNVLSFKLKLFSSDSEAYSVPEFEFDASATIPAGEFIYVPKLVGSFGDVVGVQVENKTLIFFQTGEHADSLMSFEESPARKELKIEAKEPITQEIAMKYVNLISKVVPLCKDVKISLGSGKPVFFGLHMDDFSHMKLYVAPKFESDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS11</accession>
    <name>I7AS11_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_060580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264649.1">
        <property type="nucleotide sequence ID" value="XM_009266374.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS11"/>
    <dbReference type="GeneID" id="20521455"/>
    <dbReference type="KEGG" id="ero:EROM_060580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060580"/>
    <dbReference type="HOGENOM" id="CLU_1835155_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707809" evidence="2">
        <location>
            <begin position="23"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="141" mass="14239" checksum="63D7582E8253CAF1" modified="2012-10-03" version="1">MNPVSMKFSTVMFVALSSLVSANLFWNSESTTGSSGSGSGFLGLGGSEDSKNAPSSNSSSLGEGTTEENVKDKVSGSSSSEFMGKESSNSSGLFGGSSSNSTTSKSSLAEESSCSGCDEEDKTNPGSGNTSGFWGKSKDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS15</accession>
    <name>I7AS15_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264654.1">
        <property type="nucleotide sequence ID" value="XM_009266379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS15"/>
    <dbReference type="GeneID" id="20521460"/>
    <dbReference type="KEGG" id="ero:EROM_060630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060630"/>
    <dbReference type="HOGENOM" id="CLU_624084_0_0_1"/>
    <dbReference type="OrthoDB" id="2196187at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="401"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="419"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="439" mass="50790" checksum="84AE08DFF638AE34" modified="2012-10-03" version="1">MDIYQVLVGLEKCPENYAEEYGRQLELFNSLVKLPKQPVKIIRQTLSFLLAFPKVDKRLPETLIASIGTIKCHKTRRIVVESLFRLRRKGLIDTGQLFKNLLEHHPDPKSVLRRARALVSKEAVPWILEYYGKGTDKQKGFCYYLIVYLFTKGCSGLEKEVCGGLFVDGKIGKICRMYFLDQIDFEEEGARAMELLSPEGARFGRMLFKSLQEDRMEREEKITKMRIYVLFRNRYNLKASVVRMAMGMLNPEKDDIKDLMEIIVEEVGREDVLRAIDIVGDALCSEFKDDDFVVYGLNMLREIYCKFDPEALKLKKKEDMEESTSTLSSLSGSDEGREDKENDGDSIVDKMKERIAKYIECFKGSKSKSIFYAYTSVINVMNHKKYCGRNLEFIRRKASKKERVESMKNGRADKEKYCGGKHSKGRRISRRKMNGNKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS16</accession>
    <name>I7AS16_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264659.1">
        <property type="nucleotide sequence ID" value="XM_009266384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS16"/>
    <dbReference type="GeneID" id="20521465"/>
    <dbReference type="KEGG" id="ero:EROM_060690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060690"/>
    <dbReference type="HOGENOM" id="CLU_625691_0_0_1"/>
    <dbReference type="OrthoDB" id="2191201at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="440" mass="50907" checksum="17C3E8EE0AE29843" modified="2012-10-03" version="1">MEGTKEIRRHKMLGYALTGIEGMVLVVVIGQAVRNPLGILLNPPQGFFRNLCSVRTVMLWIGICLYLIGKIGLDAGRTRITIFLYYFVSQTLFLGYFATQSSNGLTGGSMVSILPGACSIYNTILSISLSSLQAYGEDILWTRPRYTRVRGLFNYMGKSIGVVMQDMLYLYTSSTGMAIGGLGMYLTVSRTLFPWIRFGAIFQPLNETPWKTSKSIFLANMSIVAYLFLVSVVDRLLAYNMSLMNLSLANYWYKEEEDIRTLRLRFFQISELSMRYPEVLRRIARSNRSMTSLGLYIRREESEILKVMEMMRKEKEALESKMWFSVPQISQPLDKPKALVMQRRIQFVKRIRSYNFIEILGSRIGYFCKIWILINRYRSTRRSFLLVKSFMKFLEKYKGDYLLVKDLEEAFESVMKHLHNEVSDTEEVIGRGLESDLFCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS27</accession>
    <name>I7AS27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dolichyl-phosphate-mannose protein O-mannosyltransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264679.1">
        <property type="nucleotide sequence ID" value="XM_009266404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS27"/>
    <dbReference type="GeneID" id="20521486"/>
    <dbReference type="KEGG" id="ero:EROM_060900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060900"/>
    <dbReference type="HOGENOM" id="CLU_454167_0_0_1"/>
    <dbReference type="OrthoDB" id="2196365at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF53">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_67, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
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        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
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            <end position="138"/>
        </location>
    </feature>
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            <end position="194"/>
        </location>
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        <location>
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            <end position="236"/>
        </location>
    </feature>
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            <end position="529"/>
        </location>
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        <location>
            <begin position="541"/>
            <end position="559"/>
        </location>
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        <location>
            <begin position="571"/>
            <end position="594"/>
        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83182.1"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="602" mass="68726" checksum="E8F77D3D886FDA44" modified="2012-10-03" version="1"> </sequence>
</entry>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2 3">SJ-2008</strain>
        </source>
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            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="115" mass="13444" checksum="71AD6500F77055B5" modified="2012-10-03" version="1">MDYDLPFLCPILSPRYCLASTLCPCYLSSIIYARIFKNKRFSMFGFFLVPFSVYGIRRYVQDKLQYKESFETSAIKSLCCCNSLAQDLHEMKIRRIGVYKFLSEPIPCEDSDPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="131"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50800"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="537" mass="61699" checksum="1496764CB091D36C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASB7</accession>
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            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Whitman D.W."/>
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            </authorList>
            <dbReference type="PubMed" id="22802648"/>
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            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
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    <dbReference type="OrthoDB" id="550424at2759"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="J" evidence="6">
        <location>
            <begin position="7"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="CR-type" evidence="7">
        <location>
            <begin position="139"/>
            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="139"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="398" mass="44103" checksum="E93CC11A774B83C9" modified="2012-10-03" version="1">MSKDPKGYYKILELSPGASISEVRRAYSRQQAKYHLDSPFMKDKLKNAANDEEREKIKKECGEMSAKLNSAKSVLFDEKKKKEYDSGVGEFGAHFSGGGYSDIFDIFSQFTGGRGHQRTNKVNSTKYVITISLRESFVGKVSKFNVRTEKICPTCDGKGGKDVEACKKCNGSGVCTTRRNLGGFVTLAETRCDSCGGSGNKIKGKACSACNGGEYIQDKTMFEVNIKPGVRKGEKIVFEGMGDQRRNHVPGDVIFIIDVQEDSRFERRGNDLIGKIDIPLCTAIGGGVAYFTHIDGRLLEINVNPFKTFDTVLRIRNEGFKGNKVGNLVLRPNIIIGSESDRAKIMQVLPAPPRKPYGTFTKVNSEFGNMPEAEREQEDASDDGMHNAKSFFNNFSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASM7</accession>
    <name>I7ASM7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080200</name>
    </gene>
    <organism evidence="6 7">
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        <lineage>
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    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the proteasome complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264939.1">
        <property type="nucleotide sequence ID" value="XM_009266664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASM7"/>
    <dbReference type="GeneID" id="20521756"/>
    <dbReference type="KEGG" id="ero:EROM_080200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080200"/>
    <dbReference type="HOGENOM" id="CLU_035750_12_1_1"/>
    <dbReference type="OrthoDB" id="268428at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03758">
        <property type="entry name" value="proteasome_beta_type_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035206">
        <property type="entry name" value="Proteasome_beta2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 5 6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00026071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="197" mass="21953" checksum="80AD603645BE0075" modified="2012-10-03" version="1">MDSSIGIKGSNFAMVAADTRVSRSFLLVKEDYEKFQVIKDKVVMSVVGDQGDAFRTMLNVSESARYEEIQNGIEISPSVLAHMIQNKVHESLRKRQLDASTIIAGKGPEGYDLWSVDRYGAISSVPFCASGYAAYFVYGILDREYSENITIDDALKIIQKCINLLKERLIINLEGFMIKIVTDDGVLTKTLAPEIKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASN1</accession>
    <name>I7ASN1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_080250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264944.1">
        <property type="nucleotide sequence ID" value="XM_009266669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASN1"/>
    <dbReference type="GeneID" id="20521761"/>
    <dbReference type="KEGG" id="ero:EROM_080250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080250"/>
    <dbReference type="HOGENOM" id="CLU_891658_0_0_1"/>
    <dbReference type="OrthoDB" id="444945at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="313" mass="36695" checksum="6B9F74CDA8644A1C" modified="2012-10-03" version="1">MGIKKKQKSKRLTTRKRESMLKRARINEKKKRRLNRKMVLKMEKVPPFVLRTDEENMQYAEIKRMARLRKIEHDEALKNSEKKEDYLEGIMNLVSKSDVVIEVIDARDPDSSRNGEVEKVVFDYGKKLIMVLNYTQYVPRDTINEWKDYLRKDGNTCIESMEEGMEWIGKEMRIGIFGNSKSGKNFVLRSITKALGEKPRFMIASVPPSRITLTSVLRGCHKLIGMPFSSYIDAIVERIDKDEVSLRHRIPKFGNSEELLESICDRNQINDGSKCMRHMKASERFLEDFFNHRILFWRGVNDDENDISFAFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATC5</accession>
    <name>I7ATC5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265174.1">
        <property type="nucleotide sequence ID" value="XM_009266899.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATC5"/>
    <dbReference type="GeneID" id="20564284"/>
    <dbReference type="KEGG" id="ero:EROM_090600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090600"/>
    <dbReference type="HOGENOM" id="CLU_1875410_0_0_1"/>
    <dbReference type="OrthoDB" id="2190358at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="134" mass="15000" checksum="C93FDCD51CA6A20C" modified="2012-10-03" version="1">MNVRPKKSASKYSFEPKIPQSQELFEVEEPVTKKEFVFENQGEYAPKSIGQDTLEHSFILGEQEGILLTLPFDISTSKVVVYEDGSCGLKIGDKVHPMSTRFIGESLVVEHKEMAYGIGSTKFQLTAQLEGLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATH4</accession>
    <name>I7ATH4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Bacterial surface antigen (D15) domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_091360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265249.1">
        <property type="nucleotide sequence ID" value="XM_009266974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATH4"/>
    <dbReference type="GeneID" id="20564360"/>
    <dbReference type="KEGG" id="ero:EROM_091360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091360"/>
    <dbReference type="HOGENOM" id="CLU_068283_0_0_1"/>
    <dbReference type="OrthoDB" id="2189193at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial surface antigen (D15)" evidence="3">
        <location>
            <begin position="183"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="349" mass="38896" checksum="55934FABC1145DBB" modified="2012-10-03" version="1">MFKITGIKRTDKGFLDDVQKIPGSGDVDEVISRLRKLKIFSAIAKTSDGLSISEEKRSVGFSGDTTGRVPKGYLKIKLPNLFGKAENLEVSMSTSKDMSFRFTKPILWKSLSFLSISGSKDVMKSPHEEYPHTRVSLEVERENQKICAGKERIGYLDQAFIEVKQRLAAINIRSKIGVFDSRSSKLFSKTQAEMILGKSLTRSLFTDIHFSTGLILGEPHIAERYYLGENIRGYRGMSISPSNCGLKIGGNSFFEASHRMGVCKWQAKGFLFWSLGYVSENRNIVSSLKPVMRSRSYKDDECFGASVGLGASVPVMKSKDGPVVSVSFAIPLTNNRQVQKLQFGFDMDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATJ0</accession>
    <name>I7ATJ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rad2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091610</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265274.1">
        <property type="nucleotide sequence ID" value="XM_009266999.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATJ0"/>
    <dbReference type="GeneID" id="20564385"/>
    <dbReference type="KEGG" id="ero:EROM_091610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091610"/>
    <dbReference type="HOGENOM" id="CLU_027593_0_0_1"/>
    <dbReference type="OrthoDB" id="31113at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd09900">
        <property type="entry name" value="H3TH_XPG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF59">
        <property type="entry name" value="FI23547P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="8">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="7">
        <location>
            <begin position="394"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="559" mass="64056" checksum="588E8EB0A5EE4C11" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATN1</accession>
    <name>I7ATN1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Exosome RNAse PH-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_100140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265329.1">
        <property type="nucleotide sequence ID" value="XM_009267054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATN1"/>
    <dbReference type="GeneID" id="20564444"/>
    <dbReference type="KEGG" id="ero:EROM_100140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100140"/>
    <dbReference type="HOGENOM" id="CLU_123986_0_0_1"/>
    <dbReference type="OrthoDB" id="2191718at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="6">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="188" mass="21229" checksum="D96B265D14FBF0DE" modified="2012-10-03" version="1">MSRIKSMIGVIPHCTGSSRFSYNNTTVFCIVHGPSDAISRHEDPEKAFLDVRWKDMVLINGRIYDKYFSRVVERVISKNIILELDAWKTIQIIFNVVGEARNALFCAVNAALLALADGGIPLKSMFYASSSFTNEEEVVVFDNDENVIDSHSFEAISRKNIESAKGFLKYVKEVQTHTLRSKFPFSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATZ7</accession>
    <name>I7ATZ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">White ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_101420</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265454.1">
        <property type="nucleotide sequence ID" value="XM_009267179.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATZ7"/>
    <dbReference type="GeneID" id="20564572"/>
    <dbReference type="KEGG" id="ero:EROM_101420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101420"/>
    <dbReference type="HOGENOM" id="CLU_020421_1_0_1"/>
    <dbReference type="OrthoDB" id="2141921at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF91">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19055">
        <property type="entry name" value="ABC2_membrane_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="492"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="522"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="559"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="641"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="27"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="677" mass="77398" checksum="C094178C6BCD1D88" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU44</accession>
    <name>I7AU44_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_110180</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265499.1">
        <property type="nucleotide sequence ID" value="XM_009267224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU44"/>
    <dbReference type="GeneID" id="20564617"/>
    <dbReference type="KEGG" id="ero:EROM_110180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110180"/>
    <dbReference type="HOGENOM" id="CLU_071681_5_2_1"/>
    <dbReference type="OrthoDB" id="10257739at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1340">
        <property type="entry name" value="Mediator of RNA polymerase II, submodule Med31 (Soh1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038089">
        <property type="entry name" value="Med31_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008831">
        <property type="entry name" value="Mediator_Med31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13186">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05669">
        <property type="entry name" value="Med31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0010">Activator</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="12916" checksum="8ECB3E4C9066B87F" modified="2012-10-03" version="1">MSGSRFERELEFVQLLCNPEYLRWLAHEGYFESEEFRSYLRYLEYWRRPEYSRFLSYPQCLAILERLNNGNVNDIMNNESLFSALGEQQYFIWLNKHREDWNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUL5</accession>
    <name>I7AUL5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sugar permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111760</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265654.1">
        <property type="nucleotide sequence ID" value="XM_009267379.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUL5"/>
    <dbReference type="GeneID" id="20564775"/>
    <dbReference type="KEGG" id="ero:EROM_111760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111760"/>
    <dbReference type="HOGENOM" id="CLU_584071_0_0_1"/>
    <dbReference type="OrthoDB" id="515887at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF41">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="417"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="7">
        <location>
            <begin position="58"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="466" mass="53260" checksum="BEA54D226BA9BE8C" modified="2012-10-03" version="1">MESIKKINRKFLITPKILYFFVNLQYYTLHQFRGVFAKKKFGATDGDLAKYMGPMLGTIFFTNIFIGTMNDKFGRSHLFIVGALLLTCALLQLFYVEPYVGIFPGMFWINLLLYMAFNNGIPPLLDKAVLDYLNGIPEAGARAYGKQKLWGTAGYGLSCKVIEKCIKFGDEFKFGNLRYYSLVTTALSSILVFSLLKSPATEGRTARRDIMANCKELMRNGSYLFFILMILLNGITRQALSIYHTVYLTDVLQIKGYDLPSSWPYWLQYLVEFFNESPVATTTFCGMVFEVVMLYNFPAISKRIGLVWPFFIAQLFQIFRVGCYFLLDYNDPNVFLYCCLIELTRGMYFGLLQPSAVQLAMNLCPPHLKSTSQMIYHGTFTALGSLATTLVFGNLFSEDKMKGKDIPIEDRAANYKMFFFVNICFASVTTGLFVYKYFILDRISRHGTDQEKKAIIESQEEAITNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH54</accession>
    <name>I6ZH54_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264020.1">
        <property type="nucleotide sequence ID" value="XM_009265745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH54"/>
    <dbReference type="GeneID" id="20520808"/>
    <dbReference type="KEGG" id="ero:EROM_020480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020480"/>
    <dbReference type="HOGENOM" id="CLU_454939_0_0_1"/>
    <dbReference type="OrthoDB" id="2190284at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="600" mass="70133" checksum="24798F4D4E557F64" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH68</accession>
    <name>I6ZH68_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD40 domain-containing putative histone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264035.1">
        <property type="nucleotide sequence ID" value="XM_009265760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH68"/>
    <dbReference type="GeneID" id="20520823"/>
    <dbReference type="KEGG" id="ero:EROM_020630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020630"/>
    <dbReference type="HOGENOM" id="CLU_010474_0_0_1"/>
    <dbReference type="OrthoDB" id="2192933at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000417">
        <property type="term" value="C:HIR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031120">
        <property type="entry name" value="HIR1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831">
        <property type="entry name" value="MEMBER OF THE HIR1 FAMILY OF WD-REPEAT PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13831:SF1">
        <property type="entry name" value="PROTEIN HIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="50"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="658" mass="74579" checksum="BE759F7621F38FC2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH74</accession>
    <name>I6ZH74_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264045.1">
        <property type="nucleotide sequence ID" value="XM_009265770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH74"/>
    <dbReference type="GeneID" id="20520833"/>
    <dbReference type="KEGG" id="ero:EROM_020730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020730"/>
    <dbReference type="HOGENOM" id="CLU_619673_0_0_1"/>
    <dbReference type="OrthoDB" id="2194671at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="442" mass="50889" checksum="AACA82AE1FC8DA9C" modified="2012-10-03" version="1">MLDIGNPSGIIDKFKSYRSVRIFMPDMPASFCAAKIITEILHKELIRFEVDFHPEKPRFHSDSDLHLFIDLDPGECKNALLICGQKNSSIQGSLFTCTCPSKWLHSCILAYSLAKAMNYITNDILWPIITCFGFYKMFTKNTMPIDDLMSEPRFPDDVCGGCKELHEDIVLEIQKAGVLTDIDGLYHIERPCISFLNFTSLFSALQNDIPFVVDRKIHYKKDKKAECQRIHEYLARKGISKEVSCELYANLGYNTKMAIESYFRRSKCFVKKIGYDVELSSTESFFLVCYNLLKGSRVDAFLCLSGKVVPGIKKSIKIHHELIWIFRDCIANLRRLGKTLLFRINVSGVLKDMAVGVLLHLYEHYFEIYIRRRHNNSYSQMIILQGCLENKLVLATNDSFIFSELSHVKERIGEYIVMDRDELFTIIAKLKTKTRLEPSIGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH84</accession>
    <name>I6ZH84_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Spindle assembly checkpoint component MAD1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_020830</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264055.1">
        <property type="nucleotide sequence ID" value="XM_009265780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH84"/>
    <dbReference type="GeneID" id="20520844"/>
    <dbReference type="KEGG" id="ero:EROM_020830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020830"/>
    <dbReference type="HOGENOM" id="CLU_1332303_0_0_1"/>
    <dbReference type="OrthoDB" id="331602at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008672">
        <property type="entry name" value="Mad1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168:SF0">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23168">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD1 MITOTIC ARREST DEFICIENT-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05557">
        <property type="entry name" value="MAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="67"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22769" checksum="B2D459402D7F7965" modified="2012-10-03" version="1">MEELYRKFAETLKENTALRHQIVDLQSALASKEQELSMARESSGITHHLKKIESLEQENKRLRDAADKDGHRIYEGENMNPNTPASKERTESIAQASEEKDPNNELRQYLRTIREYVTGLTGYKMEFRDGEIIFHSLYAFDSEDVFIFRYKSGSMELVNNEFAASWASEIQNYLIAGKSIPAFLAAVTLELFNKKTFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHJ0</accession>
    <name>I6ZHJ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MdlB ABC transporter ATPase/permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_030140</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264130.1">
        <property type="nucleotide sequence ID" value="XM_009265855.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHJ0"/>
    <dbReference type="GeneID" id="20520921"/>
    <dbReference type="KEGG" id="ero:EROM_030140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030140"/>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_1"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034040">
        <property type="term" value="F:ATPase-coupled lipid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF654">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="30"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="341"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="584" mass="67313" checksum="F739F95933E0BC36" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHW6</accession>
    <name>I6ZHW6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">SLC41A/MgtE integral membrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_040340</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC41A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264300.1">
        <property type="nucleotide sequence ID" value="XM_009266025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHW6"/>
    <dbReference type="GeneID" id="20521098"/>
    <dbReference type="KEGG" id="ero:EROM_040340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040340"/>
    <dbReference type="HOGENOM" id="CLU_059050_0_0_1"/>
    <dbReference type="OrthoDB" id="666972at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.20">
        <property type="entry name" value="SLC41 divalent cation transporters, integral membrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006667">
        <property type="entry name" value="SLC41_membr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036739">
        <property type="entry name" value="SLC41_membr_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045349">
        <property type="entry name" value="SLC41A1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228">
        <property type="entry name" value="DIVALENT CATION TRANSPORTER SOLUTE CARRIER FAMILY 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16228:SF7">
        <property type="entry name" value="SLC41A_MGTE INTEGRAL MEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01769">
        <property type="entry name" value="MgtE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161093">
        <property type="entry name" value="MgtE membrane domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="294"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="SLC41A/MgtE integral membrane" evidence="10">
        <location>
            <begin position="57"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="392" mass="42999" checksum="8AF1A91E7B8A1268" modified="2012-10-03" version="1">MLGDLVNLSLYNTSLLLQSIPGLFISLSGLILAGSALIDATNDARLADVPIILETNCILSFKGNIEVIFAMYLSSMSHSPLFIYKNYFIYVFDNSNLVLAQSAIIGLTIGLVGIIGTTLSGELHTGLGLAIVTGSLVSCILTTILFIILLVISIEVSKILGINPDNIILPTISSFGDYMNVRSLIYFTKKFKDASIPSSLICIFLSISIIPLCLYFGLASKKRIPVQPVEVLTMTYLISTATGYVLEDFSARFPILAPSFPVFSGMSVSIAFIYLHKIFTSINNQTIHNSKESYITLVFISVLMSLTYLVIAILMGLQYSHEFCILFMIFFVFHVTILLRGISILVEYLDKKEEDTGVMALPLIASISDVLSAGFLLVIVVFLDIFGIKHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJA1</accession>
    <name>I6ZJA1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264810.1">
        <property type="nucleotide sequence ID" value="XM_009266535.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJA1"/>
    <dbReference type="GeneID" id="20521621"/>
    <dbReference type="KEGG" id="ero:EROM_070620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070620"/>
    <dbReference type="HOGENOM" id="CLU_1180208_0_0_1"/>
    <dbReference type="OrthoDB" id="2191432at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="277" mass="32510" checksum="3CE36C03EA35F565" modified="2012-10-03" version="1">MNPEEGCKELEESICWANSPPAPVPGMCYIEPQIKRDFTSFDGSLEKAIPRQLYTDLLDAMDIDFMGMVINHIEGSVQNTKNLDHPSEVLKDSERRAEVLERERIKESVNRIFDYQKYIQESFEDVDSTIIHPVDKDIQVEETYNLFPGFDEEHVIIQSDNIDFQRVSFSILEEESSQLFRRACMSDGRALTCLPQRVDDFLVFHIKNGNAYYNEVSTLYRLQEPRKKKKQTGKKGSWWSRRSKISVIQTPWDRRILETSPRPMKVLYRPRLPRKCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJC7</accession>
    <name>I6ZJC7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070920</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264840.1">
        <property type="nucleotide sequence ID" value="XM_009266565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJC7"/>
    <dbReference type="GeneID" id="20521652"/>
    <dbReference type="KEGG" id="ero:EROM_070920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070920"/>
    <dbReference type="HOGENOM" id="CLU_201331_0_0_1"/>
    <dbReference type="OrthoDB" id="2190755at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="73" mass="8402" checksum="6D4F01FA9BFAD52E" modified="2012-10-03" version="1">MGKARKAYELEITKILAKTMQSSYRKKQVEELVSRILQKIQCIIGPAKTHEEIEALKAEFMALKQEAYSLMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJU0</accession>
    <name>I6ZJU0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_081070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265020.1">
        <property type="nucleotide sequence ID" value="XM_009266745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJU0"/>
    <dbReference type="GeneID" id="20521842"/>
    <dbReference type="KEGG" id="ero:EROM_081070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081070"/>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_1"/>
    <dbReference type="OrthoDB" id="623277at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="148" mass="16870" checksum="825A1595CF8E5AE1" modified="2012-10-03" version="1">MAKMHSSGRGRSGSVKPYDTAFPTWLTKSVDEIKADVVQMGNKGIPAPDIGKRLRDEYGVGNAKDVLGCDITKFLERNGVVPKIPFDLESLVRRANTLRSHLNIYRKDNSAKYRLILVSSRMYRVARYYKRTMKIPGNWKPKLVELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK93</accession>
    <name>I6ZK93_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265180.1">
        <property type="nucleotide sequence ID" value="XM_009266905.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK93"/>
    <dbReference type="GeneID" id="20564290"/>
    <dbReference type="KEGG" id="ero:EROM_090660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090660"/>
    <dbReference type="HOGENOM" id="CLU_130561_0_0_1"/>
    <dbReference type="OrthoDB" id="2188464at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="19860" checksum="1BCED3DECD3E39D4" modified="2012-10-03" version="1">MSLPDKIIRTLKQMDRPSEFQIYRDILAEKPKLPPVEWHDLCKLVKTSKVYNILRMDLSRKEAEVLGGALKKVSLNHVDDMVDILVKKNDKNTPILLRYLLEKKKKISIDAVQRYFCEEIKRPITSKHLKLLLVMCRNYPSSISPAILDFCRSNGHPICREVLESAMDVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKB7</accession>
    <name>I6ZKB7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Autophagy-related protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">EROM_090970</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Phospholipid scramblase involved in autophagy. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(in) = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate)(out)</text>
            <dbReference type="Rhea" id="RHEA:67920"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out)</text>
            <dbReference type="Rhea" id="RHEA:38663"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine(in) = a 1,2-diacyl-sn-glycero-3-phosphocholine(out)</text>
            <dbReference type="Rhea" id="RHEA:38571"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out)</text>
            <dbReference type="Rhea" id="RHEA:38895"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 18">Preautophagosomal structure membrane</location>
            <topology evidence="3 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the ATG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265210.1">
        <property type="nucleotide sequence ID" value="XM_009266935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKB7"/>
    <dbReference type="GeneID" id="20564321"/>
    <dbReference type="KEGG" id="ero:EROM_090970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090970"/>
    <dbReference type="HOGENOM" id="CLU_670901_0_0_1"/>
    <dbReference type="OrthoDB" id="2191421at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034497">
        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007241">
        <property type="entry name" value="Autophagy-rel_prot_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038">
        <property type="entry name" value="APG9 AUTOPHAGY 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13038:SF10">
        <property type="entry name" value="AUTOPHAGY-RELATED PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04109">
        <property type="entry name" value="ATG9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0072">Autophagy</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12 18" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="13 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="274"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="325"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="384"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00024479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU364027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AFN83713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="443" mass="52062" checksum="5621BF992EC5993A" modified="2012-10-03" version="1">MVRNLKRKYLINKITLLDIYNYYGTPLSIYTSQKIRNVFVTASLCLTVYFASNMDAIRNNRIPRLGVNFSSATLVVGGVFVVKEAISYVLNLKKMASVFYIYRYILQEGIDNIRFKDVVEGVIKLEKEVHNRDLTGKDFRDIVNRNNLLLLLMMKKFPSIFRPTCSRFLLYILHSYIGKIENGPIEEINDEMRVISAAIFLLSPIISIFFILYYIVRIIEKSQNNVFYVFKKAHRPSFKYFMAKRNEYPHETRRKIQKGADYLNAFFLAKKRDCIARICSAVSFFLSCLVFLAVYLILKTVLVSNPNLNGILYQDIVLFGKTTNILYLSYFIGGMSCCLRCLSLDYASTSRRRMFIKWCVLMEQRELLKYSENRRRLATLMAKFFVPRIYLFLMEISSPFIVPFQLEKLRRRLERIHTTFNSTDIWEEYVGEIQSNRISILDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKH8</accession>
    <name>I6ZKH8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">U6 snRNA-associated ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_091830</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265295.1">
        <property type="nucleotide sequence ID" value="XM_009267020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKH8"/>
    <dbReference type="GeneID" id="20564407"/>
    <dbReference type="KEGG" id="ero:EROM_091830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091830"/>
    <dbReference type="HOGENOM" id="CLU_130474_4_0_1"/>
    <dbReference type="OrthoDB" id="10256176at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="94" mass="10530" checksum="4C4D218D25DBD681" modified="2012-10-03" version="1">MLFYEFFRSNIGKRIFVMLKAGVYVSGVLNSIDPYLNLNLLKVKILSNHPGLDSISLCSIRGSSIKYILADKDSNLLQGVNAGSRLRMVLDKCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKJ9</accession>
    <name>I6ZKJ9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_100040</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265320.1">
        <property type="nucleotide sequence ID" value="XM_009267045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKJ9"/>
    <dbReference type="GeneID" id="20564434"/>
    <dbReference type="KEGG" id="ero:EROM_100040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100040"/>
    <dbReference type="HOGENOM" id="CLU_056590_1_1_1"/>
    <dbReference type="OrthoDB" id="1555531at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003706838" description="Ribose-5-phosphate isomerase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="216" mass="23887" checksum="14ADF7C283F8F2C9" modified="2012-10-03" version="1">MLLETSPFLWFIILFWQSHMGQGCTKNKIVVGVGTGTTIERYIGALDKDVVYVPSSIQTALKLSEKGFMVSNPLAHEALDLYIDGADYFDKNGNMIKGGGGALTTEKLLHSMAKETIIIVQSCKYKERFDGCFVPIEIIPMSLMKFVSVLKEYGLNHLVRKATGKVGPLITDLGNCIVDVEYNAGFISECKHICGVVEHGLFMSNGRTIIVEEDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKT6</accession>
    <name>I6ZKT6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Karyopherin beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_101130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265425.1">
        <property type="nucleotide sequence ID" value="XM_009267150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKT6"/>
    <dbReference type="GeneID" id="20564543"/>
    <dbReference type="KEGG" id="ero:EROM_101130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101130"/>
    <dbReference type="HOGENOM" id="CLU_017822_0_0_1"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="6">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="828" mass="92825" checksum="4A159F6557A031FE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKV6</accession>
    <name>I6ZKV6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane associated phosphatidic acid</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_101330</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PA-phosphatase related phosphoesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265445.1">
        <property type="nucleotide sequence ID" value="XM_009267170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKV6"/>
    <dbReference type="GeneID" id="20564563"/>
    <dbReference type="KEGG" id="ero:EROM_101330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101330"/>
    <dbReference type="HOGENOM" id="CLU_021458_6_1_1"/>
    <dbReference type="OrthoDB" id="8907274at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008195">
        <property type="term" value="F:phosphatidate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046839">
        <property type="term" value="P:phospholipid dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043216">
        <property type="entry name" value="PAP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165">
        <property type="entry name" value="LIPID PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10165:SF35">
        <property type="entry name" value="RE23632P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="188"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="7">
        <location>
            <begin position="100"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="261" mass="29312" checksum="7678462DB5709BA4" modified="2012-10-03" version="1">MYFLYLELLIPNKCRVSDNMAKEMVIVMIVSMGLLFLSSAIEPYERPFSISDRSISKPYLRHETITFAEITAVSVVIPLVLMFVILRINTIERVYEVYFYLSFLLACLITSSIVENMKNIIGRLRPDFLSRCSPAGGKCTGNPRVILEGRRSFPSGHTSIAACGFIFLMLFASKEFGLPRLRAKMNQVFVFLLYLAFLMVPIAVGASRVMDSKHFISDVVGGGVIGVLVGVARFKRLEMDIVKERYKNVIVNDINGAENQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKX3</accession>
    <name>I6ZKX3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_101530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265465.1">
        <property type="nucleotide sequence ID" value="XM_009267190.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKX3"/>
    <dbReference type="GeneID" id="20564583"/>
    <dbReference type="KEGG" id="ero:EROM_101530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101530"/>
    <dbReference type="HOGENOM" id="CLU_709856_0_0_1"/>
    <dbReference type="OrthoDB" id="2192009at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="389" mass="45807" checksum="C3B19E90B258CCEE" modified="2012-10-03" version="1">MINFPEERLPKRLRSMFTLKMLGPPRYDGKFDFSRKKIDIPLIFPLRRSAVKSRSPKHVIFMEKIDNYNVIYSRDGYQFDIVGKYRYRFEIKDLCVRLVTGHGKAFILTGNHIYVYKQKRITMFNIQAIDLCTSPFGEIFILTTKEILIFGETGVKEVFPYTGNPMFICFYLFRELLVVTRNQVFHLDLNNLRLSTIYLTNLKIQSVVFQDRLYIQEGTKKHVRLTSLNIEDKAAESIYIENLMNISIGETIVVLYKTGGEFIFCDRFDLINAKGVVYEVENLLGFFFSENKSCFFFEERFVLWDNGSVKVIDIDLKKKEDYLMKFPENLEMLEEMYRKRDVSKGVFRPGKVISTLNEIIEEFTQKENQTVKPAPKKKIKYKEKNLGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLE6</accession>
    <name>I6ZLE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase/RNase L inhibitor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_111470</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265625.1">
        <property type="nucleotide sequence ID" value="XM_009267350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLE6"/>
    <dbReference type="GeneID" id="20564746"/>
    <dbReference type="KEGG" id="ero:EROM_111470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111470"/>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_1"/>
    <dbReference type="OrthoDB" id="6593433at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001546">
        <property type="entry name" value="RNase L inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="48"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="72"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="364"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="624" mass="70271" checksum="51E96FCE30B847C9" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS95</accession>
    <name>I6ZS95_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DnaK-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_020010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263973.1">
        <property type="nucleotide sequence ID" value="XM_009265698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS95"/>
    <dbReference type="GeneID" id="20520759"/>
    <dbReference type="KEGG" id="ero:EROM_020010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020010"/>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_1"/>
    <dbReference type="OrthoDB" id="434160at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707405" evidence="4">
        <location>
            <begin position="22"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="687" mass="76565" checksum="38D366E89A52533E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSU9</accession>
    <name>I6ZSU9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SIR2-like protein involved in telomeric silencing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264153.1">
        <property type="nucleotide sequence ID" value="XM_009265878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSU9"/>
    <dbReference type="GeneID" id="20520946"/>
    <dbReference type="KEGG" id="ero:EROM_030350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030350"/>
    <dbReference type="HOGENOM" id="CLU_021544_1_0_1"/>
    <dbReference type="OrthoDB" id="2919105at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017136">
        <property type="term" value="F:histone deacetylase activity, NAD-dependent"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF8">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="356" mass="40769" checksum="80DCFD4AB50B9568" modified="2012-10-03" version="1">MMVIDSKWIRSNIKMFSRRKVAIVVGAGISVSSGIPDFRSRSGIFNDIKKNLGISGSDLFTYSTSMSKEMRKDYLKYMSKLKDMIDRAAPSPTHEFLSLYSDVSKKFRMYTQNIDGLEEKAGLVATKDRSATVVYLHGNMKKLGCLYCGYKVDFGDTERDTYGKGEEIVCSACIRRNEKRGKDTRKRPVGVFHTTIIHYNQSHPDSSFISKMTQYDCDCDLFIVMGTSLKVFGVKKLVKYFCRLNTTRGRRILVNLDKPSREFGDLFDFFWNGDCDEFCKALNEELGLKSMAERVRRLSITKKEDCQQSPEKKTPVEGDVKIERKMIEKEVQKHIDLLIKSSKKKDSHSRVDGSQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT27</accession>
    <name>I6ZT27_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Leucine zipper domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264263.1">
        <property type="nucleotide sequence ID" value="XM_009265988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT27"/>
    <dbReference type="GeneID" id="20521058"/>
    <dbReference type="KEGG" id="ero:EROM_031440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031440"/>
    <dbReference type="HOGENOM" id="CLU_1245345_0_0_1"/>
    <dbReference type="OrthoDB" id="2187714at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="1">
        <location>
            <begin position="91"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="216" mass="25820" checksum="AE188ED2D81F3AA4" modified="2012-10-03" version="1">MEDREENIDYENEQMQATYAGYSRRPVHSGFSPTIHPYYLFRPGFLEDRMQIHQGKYGYPGEQENQFYQQIETHGYSPLQVDQERIKSIWKKERNRIAAKRCREKRIARLKELERKEHAMIYEISELKEAIHDYDNILEILLRYIQGYLDLEIGKHESFVSLFDRLCNLKKVGTPNPTYLRDVSHFLSKKLNVTNEGIDKITEAIRGWLNKLFEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT63</accession>
    <name>I6ZT63_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ribosomal RNA assembly protein KRR1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_040480</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Essential for vegetative growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Interacts with snoRNA U3. Interacts with MPP10, KRI1 and with ribosomal proteins RPS1A, RPS4A, RPS4B, RPS8A, RPS8B, RPS11A, RPS11B, RPS13, RPS24, RPS25, RPL4A, RPL7B, RPL8, RPL23, RPL25 and RPL28.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264313.1">
        <property type="nucleotide sequence ID" value="XM_009266038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT63"/>
    <dbReference type="GeneID" id="20521112"/>
    <dbReference type="KEGG" id="ero:EROM_040480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040480"/>
    <dbReference type="HOGENOM" id="CLU_040185_1_0_1"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="K Homology" evidence="14">
        <location>
            <begin position="101"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="212"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="246"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="281" mass="32899" checksum="6B620AC09962F729" modified="2012-10-03" version="1">MNQDVKFSEKDFKHEFVEMSSEAVMFPKYREKYIEQTQKYIIKALEGKKISCKIDLEKRVIDVETNKSTRDPFIFIKAVNFVKLVSRGVGVEEAMKVLEDEYFCEVIDIKKLASSDKVFEKRRDRLIGPKEMTLKAIQILTKCYVLVHGKTVSIIGSFRGIEEVKKIVIDCMNNIHPMYQIKKLVEKRKLEGDITKEGEDWSRFLPEIKKSNKKSKKKIIGRHSGNMPSDIPKRKEDIEMETGEYFTDRELSGRKESGGERKKRKEEKKEKEIEKYAVPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT84</accession>
    <name>I6ZT84_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sm protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_040730</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the snRNP Sm proteins family. SmF/LSm6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264338.1">
        <property type="nucleotide sequence ID" value="XM_009266063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT84"/>
    <dbReference type="GeneID" id="20521137"/>
    <dbReference type="KEGG" id="ero:EROM_040730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040730"/>
    <dbReference type="HOGENOM" id="CLU_076902_12_2_1"/>
    <dbReference type="OrthoDB" id="429711at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034715">
        <property type="term" value="C:pICln-Sm protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01722">
        <property type="entry name" value="Sm_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016487">
        <property type="entry name" value="Lsm6/sSmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034100">
        <property type="entry name" value="Sm_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021:SF0">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11021">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN F SNRNP-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006609">
        <property type="entry name" value="snRNP_SmF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="11">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="81" mass="9197" checksum="85D4C94635349125" modified="2012-10-03" version="1">MNEACDKKPPKENPKQFLQRMKNRNVSVCLKWGQVYEGVLVTSDNYFNVLLDECVEKEGETSTKVGEVSIRCNNIKSISEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTB2</accession>
    <name>I6ZTB2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_041090</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264373.1">
        <property type="nucleotide sequence ID" value="XM_009266098.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTB2"/>
    <dbReference type="GeneID" id="20521173"/>
    <dbReference type="KEGG" id="ero:EROM_041090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041090"/>
    <dbReference type="HOGENOM" id="CLU_037229_4_0_1"/>
    <dbReference type="OrthoDB" id="5377392at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000706">
        <property type="term" value="P:meiotic DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="11">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="12">
        <location>
            <begin position="138"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="10">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="301" mass="34753" checksum="8AE624C87D9E566E" modified="2012-10-03" version="1">MTSTSTKISKSLRYSTLKLLTDLKSRTLVVRIRLHEIIIEMQESGISRNEREIFYMDVNVFRTQTTVKRMISSIASELRVSKYELGVRNTLKGTFIGRLTFIKCGSLSAMEICSPENTPQLIPDMSKVKEVLCDYKKVLVVEKDTILQRIASEIGRERCLEGVLLVCGRGYPCKNTMLLLKMIENKTTISGFFDFDPFGIHIFCIYKHGSRMDPDTRVETITRIGVCVEDALERNKWEGGFLELSDHDLKMIDKLMKFKDLASDLLFLKKTNKKIEMEAFFSKTSEELRHFLCKALRRMKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTN2</accession>
    <name>I6ZTN2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">40S ribosomal protein S9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050950</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264523.1">
        <property type="nucleotide sequence ID" value="XM_009266248.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTN2"/>
    <dbReference type="GeneID" id="20521327"/>
    <dbReference type="KEGG" id="ero:EROM_050950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050950"/>
    <dbReference type="HOGENOM" id="CLU_089738_1_0_1"/>
    <dbReference type="OrthoDB" id="1697570at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="7">
        <location>
            <begin position="112"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="185" mass="21308" checksum="3870738716F1DCAC" modified="2012-10-03" version="1">MANKKGSKRANTPRDPFEKERLIREIQLVGVYGLKNKHELWVMEKMFSKDKERARMLLTSTNPEDIPIDGRSLLKRLMKYGILNGIDLCDKQKVIEGLNKVLDLTINHYLERRLQFRVFAAGLAKSVHHARVLIKGRCISIKDQVVDVPGFMVRADKEPLIEYNPYSRYGDKGKKKKAGAKVDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTP0</accession>
    <name>I6ZTP0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit CBF5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">H/ACA ribonucleoprotein complex subunit cbf5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_051050</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a uridine in mRNA = a pseudouridine in mRNA</text>
            <dbReference type="Rhea" id="RHEA:56644"/>
            <dbReference type="Rhea" id="RHEA-COMP:14658"/>
            <dbReference type="Rhea" id="RHEA-COMP:14659"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine in snRNA = pseudouridine in snRNA</text>
            <dbReference type="Rhea" id="RHEA:51124"/>
            <dbReference type="Rhea" id="RHEA-COMP:12891"/>
            <dbReference type="Rhea" id="RHEA-COMP:12892"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264533.1">
        <property type="nucleotide sequence ID" value="XM_009266258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTP0"/>
    <dbReference type="GeneID" id="20521337"/>
    <dbReference type="KEGG" id="ero:EROM_051050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051050"/>
    <dbReference type="HOGENOM" id="CLU_032087_3_2_1"/>
    <dbReference type="OrthoDB" id="10250002at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="9">
        <location>
            <begin position="16"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="263"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="357" mass="40445" checksum="B93A84553C1897D5" modified="2012-10-03" version="1">MTENLKYFMEDKGLSTSETWDLLLADMDKMVVRTNHYTPLGCGSMPLNRKIGEYIKYGIVNLDKSSNPSSHEVVTWVKEILKCEKTGHSGTLDPQVSGVLTICIDRATRLTKSQQSLGKEYVCVIEFLEEPNEAEFHKISKRLIGSLLQRPPLMCAVKRELRVRNIYNIDILEFSKEKRVGLFKVSCEAGTYVRTLCTHMGILMGCGAKMKELRRTRSGRTSEKDIFTLHDLLDAVHIFNSEKDETVIRRVIRPLESLLVGYPRIMIKDSCVNAICYGAKLSVTGVLRFDRNIDVGKEIVIITTKGEAVALGIALVSSPEMSIIDHGLVCRTKRVIMEKDLYPRSWGFKNEYEILSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUF2</accession>
    <name>I6ZUF2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Adrenodoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_070550</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264803.1">
        <property type="nucleotide sequence ID" value="XM_009266528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUF2"/>
    <dbReference type="GeneID" id="20521614"/>
    <dbReference type="KEGG" id="ero:EROM_070550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070550"/>
    <dbReference type="HOGENOM" id="CLU_082632_5_0_1"/>
    <dbReference type="OrthoDB" id="268593at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426:SF65">
        <property type="entry name" value="FERREDOXIN-2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="FERREDOXIN/ADRENODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="41"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="157" mass="17129" checksum="F13D4F5878359647" modified="2012-10-03" version="1">MYEASQGTGLYSLFPPLKFLSVIFALDLPMAMSYAPDRASGKIKLFFKAMGQMVPVKAVYGDTVLETAHKNGVNLEGACEGNLACSTCHVILEESLYKRLGEPSDKEYDLIDQAFGGTSTSRLGCQLKVDKNFENAVLTIPRATKNMAVDGFKPEPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUP8</accession>
    <name>I6ZUP8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_080130</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat SEC31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264933.1">
        <property type="nucleotide sequence ID" value="XM_009266658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUP8"/>
    <dbReference type="GeneID" id="20521749"/>
    <dbReference type="KEGG" id="ero:EROM_080130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080130"/>
    <dbReference type="HOGENOM" id="CLU_320311_0_0_1"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="634"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="649"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="675"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="734"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="799"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="952" mass="105010" checksum="BD6F3688C9CD12AA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUQ6</accession>
    <name>I6ZUQ6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit K</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_080240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264943.1">
        <property type="nucleotide sequence ID" value="XM_009266668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUQ6"/>
    <dbReference type="GeneID" id="20521760"/>
    <dbReference type="KEGG" id="ero:EROM_080240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080240"/>
    <dbReference type="HOGENOM" id="CLU_112527_2_0_1"/>
    <dbReference type="OrthoDB" id="259769at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="13042" checksum="6A242D689968B336" modified="2012-10-03" version="1">MDSEDVQSASEYSVEEKSMEVLGTSNTDVQIRGSERKTLPRMTKYEKAHVIGVRASQLSAGAPPFVDIGNESNPLNIANMELNEKKIPFIIRRRLPDNSFEDWAIDELMIPGVDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUS8</accession>
    <name>I6ZUS8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_080500</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264968.1">
        <property type="nucleotide sequence ID" value="XM_009266693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUS8"/>
    <dbReference type="GeneID" id="20521786"/>
    <dbReference type="KEGG" id="ero:EROM_080500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080500"/>
    <dbReference type="HOGENOM" id="CLU_084834_0_0_1"/>
    <dbReference type="OrthoDB" id="654211at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000125">
        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="249"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="185"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="295" mass="33865" checksum="1958268927C815F4" modified="2012-10-03" version="1">MKQGTGQDLRLWVESVVSRVSSGTDINEVLLPNTRIKIYVFFVNGRVLFNLSDLASVFKCFGVCASPSQIREYMRSAGHVEKLERFKDFFGEQGLEVVCSDAINTKVLESMESFMYDGKRPHQLEGVDKELSSPGLSCGEYKLETQKKGFEGFHNVNELYPFNCQWTDSIMDGILKNIYTSDGKRAESNSISKERSDSTKPGSRGRHMKYSGSLKDRPFVCTYNECKRAFKRYEHLKRHNLMHTGERPHKCKFPGCSKAFSRSDNLSQHYKVHTTTTEMHAKSYGSYRYLNKEFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUY5</accession>
    <name>I6ZUY5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 9">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7 9">2.1.1.216</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_081450</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7 9"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265058.1">
        <property type="nucleotide sequence ID" value="XM_009266783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUY5"/>
    <dbReference type="GeneID" id="20521880"/>
    <dbReference type="KEGG" id="ero:EROM_081450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081450"/>
    <dbReference type="HOGENOM" id="CLU_010862_5_0_1"/>
    <dbReference type="OrthoDB" id="6349953at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 9" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="432" mass="49034" checksum="08AAA2A778D96A19" modified="2012-10-03" version="1">MEAKIDAKEVILEESTVITKNEWTFFNPAQKLNRDISVELIKEYFKDKATIRILDAMSATGLRGIRYLKEISNSTVYLNDISQSSIETIKSNVLLNGIEDMEYFESNLQEIRTSGNTRVNIIKSDCNVLMGSLPCFFDVIDIDPFGSCSEYIDNALRAIKHRGIICLTATDKGVLCSNERKCLIKYSTAIMRGVGMNETPLRTIVSLVSRQASKFGCSVEPVMSLSIDFYVRIFLRIVRRNPRSVIERNSFVLMCRCHNITEISNVQKEIDNRCSNCGGRMKLCGPFWNRALNDTSVIKNVLNNTSRDNKRLIGILRYLEQELPNMFYYEIPRLCSILGISTIKQSKLMHALANMGYLVSYTHSELNAIKTNAPIDSINKVLLLSNSNDQERIKEAGIRFDENESVKALEAMEFFKGLLKSGMGPLSLPRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVS4</accession>
    <name>I6ZVS4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">26S proteasome regulatory subunit S5A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100290</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265343.1">
        <property type="nucleotide sequence ID" value="XM_009267068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVS4"/>
    <dbReference type="GeneID" id="20564459"/>
    <dbReference type="KEGG" id="ero:EROM_100290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100290"/>
    <dbReference type="HOGENOM" id="CLU_098733_0_0_1"/>
    <dbReference type="OrthoDB" id="1731724at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0647">Proteasome</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="208" mass="23875" checksum="8FC1CF86B7220CFE" modified="2012-10-03" version="1">MPETIVVLFDNGMASQNQDYLPSRLIVQKETVESLISRKFEDNQENTIGIIPLVQTQSNDIITPTKQRPYIKTFLNEIKLSRNVDIMGCLSQSLHIFNQKDSPGCMLVIFLGSEPPETEKDELFARLYQLLTFGIMIKMVFFGEAIEMAEAYKKIDFTNFSCIEVKPSEEFADRVLSFINGDEHLEEDDPELAEAIRLSLEEQKKQQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVX2</accession>
    <name>I6ZVX2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265403.1">
        <property type="nucleotide sequence ID" value="XM_009267128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVX2"/>
    <dbReference type="GeneID" id="20564520"/>
    <dbReference type="KEGG" id="ero:EROM_100900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100900"/>
    <dbReference type="HOGENOM" id="CLU_1272293_0_0_1"/>
    <dbReference type="OrthoDB" id="2190045at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51543">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51542">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="25560" checksum="E8895FD031268FFB" modified="2012-10-03" version="1">MNGEESGKECSMQQKRLMGALRRRDFLKEEMEKIGQDLQKLEAHRRVLVDILRICDGRMEIDVNRYRPVYKIDLSHSKGLIHIPQEKHHLVIYNLGKIPSPSMKPFYSQKNVYPVDYMCKRAYYGKRDELLAGKREVLYTCTIRNVCSKPLFEITEGDLCIRGKAGEVFNAFRSLFPEGIEFTSIIEFFGLNNLHVRKLISEQEGFADLGIGDRWERRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW54</accession>
    <name>I6ZW54_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265488.1">
        <property type="nucleotide sequence ID" value="XM_009267213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW54"/>
    <dbReference type="GeneID" id="20564606"/>
    <dbReference type="KEGG" id="ero:EROM_110070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110070"/>
    <dbReference type="HOGENOM" id="CLU_1012039_0_0_1"/>
    <dbReference type="OrthoDB" id="2191424at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="276" mass="32057" checksum="A2FFC32A80C1020E" modified="2012-10-03" version="1">MEKNYIDRVRRALEVFPESKDEERKRRRCGKARSLIIAERTYSISWDHTFSTNYKILYPKEFVREIDKESIIKSSSVIGECNGTPIETDAANNVYYEAECLLRGEVVARLTISGDTHLSDLGGFLIGSNSVSSKVFFAIEKTIYLQTIDDNSIKFATMVFQLKIFNIKKWDIRIDKCIKDISKEIYFHDGRFLKSVQIMKAFFSYRKISPYVSVKHLYGRAQLCKCCLKNNAVLKIANDPILPEKKKFICKKCFELLFLDKEGDSRYDGIEVEFLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW82</accession>
    <name>I6ZW82_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_110390</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265518.1">
        <property type="nucleotide sequence ID" value="XM_009267243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW82"/>
    <dbReference type="GeneID" id="20564638"/>
    <dbReference type="KEGG" id="ero:EROM_110390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110390"/>
    <dbReference type="HOGENOM" id="CLU_032621_0_1_1"/>
    <dbReference type="OrthoDB" id="10261385at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.240.10:FF:000007">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF1">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN84021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="385" mass="44225" checksum="0F1182E8BB6C6CB7" modified="2012-10-03" version="1">MAEQKITPWEVEVVSTDETPVAIDYDKIINQFGCEKFDQALVDRLEGLTNSSPHYFFRRGIVFAHRDFNHLLNEMANNKSFYLYTGRGPSTKAMHIGHVVPFLLCKYVQDTFRIPLVIQMTDDEKFLWKNIKLEDAIKNGKENVKDIVALGFDPKLTYIFSNVESSHRFEKNVLKISKTINLNEASKVFGFNMSSNIGQIAFPSKEIAPCFSSSFKFIEKEAICLVPAAVDQDPYFRLARDKAKILGEKKPVSVYVSLLPDLKGVNRKMSASDPNSSIYLDDSQETIRRKITVHAYSGGRKTLEEHREKGGNIDVDVPFEYLRYFLDDDEELEKYRSGYTKGEITSKEMKEKCIAVIQDFVSKYQEARKKVTEEDVKAFMDINKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADE1</accession>
    <name>I7ADE1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="12">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="12">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ALA1</name>
        <name evidence="14" type="ordered locus">EROM_021400</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="12">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Alax-L subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="12"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264112.1">
        <property type="nucleotide sequence ID" value="XM_009265837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADE1"/>
    <dbReference type="GeneID" id="20520902"/>
    <dbReference type="KEGG" id="ero:EROM_021400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021400"/>
    <dbReference type="HOGENOM" id="CLU_004485_5_0_1"/>
    <dbReference type="OrthoDB" id="2423964at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070143">
        <property type="term" value="P:mitochondrial alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000011">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 12" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="13">
        <location>
            <begin position="3"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="553"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="557"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="656"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="660"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="864" mass="98695" checksum="B4DAC27C0A25CD02" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADE8</accession>
    <name>I7ADE8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0328 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264117.1">
        <property type="nucleotide sequence ID" value="XM_009265842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADE8"/>
    <dbReference type="GeneID" id="20520908"/>
    <dbReference type="KEGG" id="ero:EROM_030010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030010"/>
    <dbReference type="HOGENOM" id="CLU_2512628_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019081">
        <property type="entry name" value="UPF0328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09591">
        <property type="entry name" value="DUF2463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="85" mass="9778" checksum="D2BB87D3AE4D016F" modified="2012-10-03" version="1">MVIALKGQEGILSYFAITSIALVLIRSLKQKYSQSPKHEGLYTWWRLTILTIILIIIVYGFLISVCQNALYTYFNLYIKCNLTNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADR2</accession>
    <name>I7ADR2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAR-17a/AIG1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264212.1">
        <property type="nucleotide sequence ID" value="XM_009265937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADR2"/>
    <dbReference type="GeneID" id="20521005"/>
    <dbReference type="KEGG" id="ero:EROM_030940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030940"/>
    <dbReference type="HOGENOM" id="CLU_103036_0_0_1"/>
    <dbReference type="OrthoDB" id="1898221at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="26370" checksum="178B9FCB22FEFFBA" modified="2012-10-03" version="1">MEKRKTRSVLKEALKLVFLGFCVYATTDFSLPPEIVEMSNATSGGKFVYLTIITLYLTIISTVLGYISKTRVGKRLEEIYKDMLAVTFSLEGIVTTLFWSLYFINPTLLKNKSLYKRGIRESFLTELSAHLFPIILLLICQADVRLQKRKRCIYFIFGFGALYFLEICYFSFVNNGKWAYPIFRKMGWVQRILFVLIACFIGTLFYMGLLATNSLIYQEYEQPNEND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADW2</accession>
    <name>I7ADW2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_040150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264282.1">
        <property type="nucleotide sequence ID" value="XM_009266007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADW2"/>
    <dbReference type="GeneID" id="20521079"/>
    <dbReference type="KEGG" id="ero:EROM_040150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040150"/>
    <dbReference type="HOGENOM" id="CLU_1348918_0_0_1"/>
    <dbReference type="OrthoDB" id="1937934at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="2">
        <location>
            <begin position="130"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="204" mass="23532" checksum="4E2E6233DC35440B" modified="2012-10-03" version="1">MDKEIPEPKDIALTSNEFLEYLKGKYKKEVRREHNKIIFAREPLKYRKMGLNEYLEKDRESQEEIVIPKMNMSELMILGQEANVSISIDKFNKDPEIMVLLVRGTITGIESFREKMEYIHNKLPGNIQTDGEVKVLDIPPESEDIVIGKNGVNIYKLQKDFKIIIRVIDGNRSGIKALVISGDDKSQVQEACNHVINLIYRNVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADY2</accession>
    <name>I7ADY2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_040420</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 18S rRNA in the 40S particle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine(1779)/adenosine(1780) in 18S rRNA + 4 S-adenosyl-L-methionine = N(6)-dimethyladenosine(1779)/N(6)-dimethyladenosine(1780) in 18S rRNA + 4 S-adenosyl-L-homocysteine + 4 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10234"/>
            <dbReference type="Rhea" id="RHEA-COMP:10236"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74493"/>
            <dbReference type="EC" id="2.1.1.183"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="9"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264307.1">
        <property type="nucleotide sequence ID" value="XM_009266032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADY2"/>
    <dbReference type="GeneID" id="20521106"/>
    <dbReference type="KEGG" id="ero:EROM_040420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040420"/>
    <dbReference type="HOGENOM" id="CLU_041220_2_3_1"/>
    <dbReference type="OrthoDB" id="74991at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052909">
        <property type="term" value="F:18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000081">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="10">
        <location>
            <begin position="20"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="277" mass="31697" checksum="9D770AA2EE6CD755" modified="2012-10-03" version="1">MVNPKFNKQHGQHILKNLGVIDTIIERARIKPTDVVLEVGGGTGNLTMKLLQKAKKVICYEIDPRLAAELAKRVNGVPGMAQKFQLFVGDALKHDFPHFDLCITNLPYQISSPFMFKLLKYNFKCAYIMFQREFSDRLVARPGNPEYCRLSVGAQMLAQIDHVLKVSKNSFVPPPKVESSFVRIEPRIPRPPIDFEEFDRFLKICFLRKNKTLRANFKNSSLVNKIKADPVFENTTPEDVFERVLSKADLDGARAAKMDLDDFLALLLEFKKINIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE82</accession>
    <name>I7AE82_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264447.1">
        <property type="nucleotide sequence ID" value="XM_009266172.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE82"/>
    <dbReference type="GeneID" id="20521249"/>
    <dbReference type="KEGG" id="ero:EROM_050170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050170"/>
    <dbReference type="HOGENOM" id="CLU_663972_0_0_1"/>
    <dbReference type="OrthoDB" id="2190084at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="425" mass="47695" checksum="05038D8C3FCA420A" modified="2012-10-03" version="1">MFFLLLFSSNKHMENTRSRVCSTLGQTYKIVGTSPLVPVFLSLTYFTKGIKTGYYTYGIIASGMLLGLSLYIIEYVGKKRSEESVKSLGDMSKGVSRILSEAVDVFVVASKYTTLLITVDVFFNVLFIYFPFLDEIRKSMAIYKILFGLGSALVGLTLYVFSTFTRKMFLIASASASILFVTTVLVGYFLGMNSALPKPSYSQGSMLPSFFWVTTIAFYTMEFISGGVKIIGESQHNENGKYVAISAMLAVPGMYLILLHSAGWIRSSYNSLDILNLIFSKSSKLNVYLRKYTIDKYCITTKAMCIFVPIICISLFVIQFDGFVESFKNLVSAESDNKLWVLSLFMLSVVFIQVLFNEIIGSFHFYMVLIVIGITPLLVYHPFICYIFASRKVRFFTAVCILAMIFTSATLVGTVRTLIDILQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEX9</accession>
    <name>I7AEX9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_061230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264712.1">
        <property type="nucleotide sequence ID" value="XM_009266437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEX9"/>
    <dbReference type="GeneID" id="20521519"/>
    <dbReference type="KEGG" id="ero:EROM_061230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061230"/>
    <dbReference type="HOGENOM" id="CLU_1481975_0_0_1"/>
    <dbReference type="OrthoDB" id="2193810at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="3">
        <location>
            <begin position="14"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="1">
        <location>
            <begin position="14"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="161"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="201" mass="23354" checksum="1FD032783A4938F3" modified="2012-10-03" version="1">MSMEREAKTLYEMPTKQRSPYSVFAREFFRNNKHEYTSHVDLAKALVKTWNEMQDEEKSTYVEKCNKSLEDASELAVASEESPIPYDKDNLKKLKKYIKAGPTYRWEYSGYLSFVNEIFDICGDMTVSPEENVRMFAEMWESLEDEVQNKYRNKALGSLAAKKKPRKRNMGNALRKSDKIYTTSEGLEPSTFGSVGRHSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEZ0</accession>
    <name>I7AEZ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_061390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GINS2/PSF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264727.1">
        <property type="nucleotide sequence ID" value="XM_009266452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEZ0"/>
    <dbReference type="GeneID" id="20521536"/>
    <dbReference type="KEGG" id="ero:EROM_061390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061390"/>
    <dbReference type="HOGENOM" id="CLU_078274_3_1_1"/>
    <dbReference type="OrthoDB" id="1938138at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11712">
        <property type="entry name" value="GINS_A_psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21694">
        <property type="entry name" value="GINS_B_Psf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021151">
        <property type="entry name" value="GINS_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
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    </dbReference>
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    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
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            <begin position="2"/>
            <end position="60"/>
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    <feature type="domain" description="GINS subunit" evidence="5">
        <location>
            <begin position="65"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010565"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25005"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="173" mass="19867" checksum="92CBC7FBD332DE87" modified="2012-10-03" version="1">MSPEEILHIAYEELVEVEPMTSIPELCLMDKTYPPMMPLDVVRIPLYAALLLKKSNMCKIRLPSYLQLESLKAIMDTEVEKIDEYSHIHPYFFPLAGELLECCYNTENIEESKVMIEKIREIRLAKTLKGIKCLDGRALNMNNITLFEFNEVKELILGSAEVGRKIEGLSKSQ</sequence>
</entry>
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    <accession>I7AEZ9</accession>
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        </submittedName>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
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                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855:SF3">
        <property type="entry name" value="LD03007P"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="176"/>
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    </feature>
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            <end position="212"/>
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    </feature>
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        <location>
            <position position="58"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
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        <location>
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        </location>
        <ligand>
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        </ligand>
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        </location>
        <ligand>
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        </ligand>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="218" mass="24938" checksum="2A2BB97DFB50ADBF" modified="2012-10-03" version="1">MSEQKPILRGRIHQVAFYLTIAKTFMYAICCIHRKGNFGIMIYLMSQLVLFGVSSTYHVTSWKDEKTKSLFQRLDHISIFLLISGTQTSVILNLVPTGMYTKYILMMTWTISLAGILKIILMKKLHMHFDLMVYILHGVSIVPFFRIIMNSIPISDLSLFILGGAIYIIGGLIYGMERPNPYPQVFGYHEIFHVMTVLANWCFLVPLLKGYISTLSGK</sequence>
</entry>
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    <accession>I7AF26</accession>
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        <submittedName>
            <fullName evidence="3">Membrane protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">EROM_070240</name>
    </gene>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
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    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="272139at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
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    </dbReference>
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        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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    </feature>
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        <location>
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        <location>
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        <location>
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        <location>
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        <location>
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            <end position="721"/>
        </location>
    </feature>
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        <location>
            <begin position="166"/>
            <end position="241"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="725" mass="83347" checksum="7B64F27D1259FE95" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF54</accession>
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        <recommendedName>
            <fullName evidence="2">Cdc37 N-terminal domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="3" type="ordered locus">EROM_070590</name>
    </gene>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83310.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266532.1"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="440202at2759"/>
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        <property type="component" value="Chromosome VII"/>
    </dbReference>
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        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013855">
        <property type="entry name" value="Cdc37_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03234">
        <property type="entry name" value="CDC37_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc37 N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="213" mass="25294" checksum="331A2ECF8CF0BF01" modified="2012-10-03" version="1">MSSKYSGINLDSDEEEEIHPNIDEKSYKEWKRRQREQKRRELEQRLQEINSINDPGEEMIEEKNEIERILKPSYVCLDTESFRIPSEDSEKDYIQELEYLIVHNELDNFIELMNQSKINMEKFEVVVLHNLAEQIKEGNDAGGLILSKISLYAKYALSHGKDFLLKLRAQLTNKEKKKQFEEDCRKDFEETKRMFLEEFGEHVSAKQPSCPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF87</accession>
    <name>I7AF87_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ricin B lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264852.1">
        <property type="nucleotide sequence ID" value="XM_009266577.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF87"/>
    <dbReference type="GeneID" id="20521664"/>
    <dbReference type="KEGG" id="ero:EROM_071040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071040"/>
    <dbReference type="HOGENOM" id="CLU_1107120_0_0_1"/>
    <dbReference type="OrthoDB" id="2192475at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="29074" checksum="8446D8223921848F" modified="2012-10-03" version="1">MIMALLAGVALSYPLYLDSFTNKPIRIVAMEDKNLLASEFVKDFGTNPFRVIDILNPANGYNTRSQINRNGPVFKVRVGGSWLCRNRRDLSKCADNTDFWEITRSGDGFTISQEGYCLSTEGKGRLILERCNGRSRSHLFLFEDMEIEDCMDSVDLGGQPRTEAEKVKQLKLKKKLDDLKKKDRAKAEKIKKKLEEKNDFEKYAKKNLPDLTGKDDSKEVLSKLWDFGWKRPKFSFPSFPWFKFPFCKKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFH9</accession>
    <name>I7AFH9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080550</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264972.1">
        <property type="nucleotide sequence ID" value="XM_009266697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFH9"/>
    <dbReference type="GeneID" id="20521791"/>
    <dbReference type="KEGG" id="ero:EROM_080550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080550"/>
    <dbReference type="HOGENOM" id="CLU_080773_2_0_1"/>
    <dbReference type="OrthoDB" id="1294322at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002190">
        <property type="entry name" value="Ribosomal_L18a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="5">
        <location>
            <begin position="7"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="173" mass="19837" checksum="082671EA302238B2" modified="2012-10-03" version="1">MTTPITKEYRIHGSAMPTEGDPRPQIFVATIFTKNHVFAKAKFFKILEKKYKIKASKGLIINCERIPEPSFKEVQNYGIRFIYRSRSGVHNAYKECRAISRCGAVDHVLRELAGRHKLKRSAVDIISVEAIPFESLRRSKTMEFADENVEFPVFTKILNTKSLLIPSDYNPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFL7</accession>
    <name>I7AFL7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265027.1">
        <property type="nucleotide sequence ID" value="XM_009266752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFL7"/>
    <dbReference type="GeneID" id="20521849"/>
    <dbReference type="KEGG" id="ero:EROM_081140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081140"/>
    <dbReference type="HOGENOM" id="CLU_109938_0_0_1"/>
    <dbReference type="OrthoDB" id="264354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="1">
        <location>
            <begin position="4"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20667" checksum="B0B729D0437A0B89" modified="2012-10-03" version="1">MGFLNKLVITVKKPTKRSKQMCMHIRRMLEPNVTSKLRDKNTTVRSYLDVADILELSHFILVDARDIKIGVRPKGPTYVFNVIDYNPKYVMVGSEYYKDDPCITFTGESDTKELFMSLSSQPKTFKRNLHFYFDGDLIHVRHYAIVTKEEEDIKVGFNEIGPRITMKLIKKMDGFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFT8</accession>
    <name>I7AFT8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyclin-dependent kinase 1</fullName>
            <ecNumber evidence="2">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_081910</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265102.1">
        <property type="nucleotide sequence ID" value="XM_009266827.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFT8"/>
    <dbReference type="GeneID" id="20521926"/>
    <dbReference type="KEGG" id="ero:EROM_081910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081910"/>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="OrthoDB" id="1732493at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030332">
        <property type="term" value="F:cyclin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010389">
        <property type="term" value="P:regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07829">
        <property type="entry name" value="STKc_CDK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000574">
        <property type="entry name" value="Cell division related protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000375">
        <property type="entry name" value="Cell division related protein kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF254">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="5"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="34385" checksum="DC1DDCEDEA65BE6B" modified="2012-10-03" version="1">MTESFQKLEKIGEGTYGVVYKARERNTDRIVALKKIRLENENEGIPATTIREILLLKNLKHSTIVELSDVIYNNNKMYLVFEYVELDLRRYLDKMNDEGRLADEGFVKKMSHQLLTAMEYCHSRNIFHRDLKPQNILIDPKENIKLADFGLGRAAGVPLRTYTTEVVTLWYRPPELLLGCKYYDASVDVWSAACIMAEVVLMKPFFPGDSEIDQLFRIFKVLGTPSNSRWSNVENFPNYKVEFPIWDPVDLKTILKADSDFIDLISKMLEYDPKLRMTAKSGLSHRYFKNIPPILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG80</accession>
    <name>I7AG80_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265257.1">
        <property type="nucleotide sequence ID" value="XM_009266982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG80"/>
    <dbReference type="GeneID" id="20564368"/>
    <dbReference type="KEGG" id="ero:EROM_091440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091440"/>
    <dbReference type="HOGENOM" id="CLU_2704788_0_0_1"/>
    <dbReference type="OrthoDB" id="2195410at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031509">
        <property type="entry name" value="Mei5-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17021">
        <property type="entry name" value="Mei5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="73" mass="8756" checksum="FEB77F79C6045426" modified="2012-10-03" version="1">MMRQFRETHGNLESVIERWKECISECISILCNQYNIPPLEIFKAFPLRKWGFDIEEYGGCEEDLLQNNEDQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGD2</accession>
    <name>I7AGD2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_092060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0328 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265317.1">
        <property type="nucleotide sequence ID" value="XM_009267042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGD2"/>
    <dbReference type="GeneID" id="20564430"/>
    <dbReference type="KEGG" id="ero:EROM_092060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_092060"/>
    <dbReference type="HOGENOM" id="CLU_2015250_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019081">
        <property type="entry name" value="UPF0328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09591">
        <property type="entry name" value="DUF2463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="123" mass="14888" checksum="67C3BDF1C255BF8B" modified="2012-10-03" version="1">MKPGNVLEVCVTDESHDNKGKIHLYWKMIIQYFIGPISVLFPIFIYYMASEDLFNRSIPLRLTTVILPFSYSAERHFFLFHKNWMSAYRFELKLDKILYSILNLFFLAFFYYLYTIDYFVLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGF4</accession>
    <name>I7AGF4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S23</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_100280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265342.1">
        <property type="nucleotide sequence ID" value="XM_009267067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGF4"/>
    <dbReference type="GeneID" id="20564458"/>
    <dbReference type="KEGG" id="ero:EROM_100280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100280"/>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_1"/>
    <dbReference type="OrthoDB" id="1713912at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="140" mass="15435" checksum="1BC8260DAC8B1378" modified="2012-10-03" version="1">MSGLFSANNLIKNRKAKRLADTVYRKRALGTKYKHSVLGRAPQAKAIVLEKIGVEAKQPNSAIRKAVRCQLIATGKRITAFVPYDGSVTYIESNDEVTVEGFGKKGRSFGDIPGIRFKVCKVQNVSLHAIFTGKKEKPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGG6</accession>
    <name>I7AGG6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribosomal protein S6 kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_100440</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265357.1">
        <property type="nucleotide sequence ID" value="XM_009267082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGG6"/>
    <dbReference type="GeneID" id="20564474"/>
    <dbReference type="KEGG" id="ero:EROM_100440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100440"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_5_1"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="336" mass="39273" checksum="16B3F73B9BD34AAF" modified="2012-10-03" version="1">MIRKEDIMCIEKTGSGPFWEEFLVFIEVRKRVGGLYHLRVMKKKKIIDYGECDMIQNEYYIRSRIRHPFLINTICGFQDYEHLFLVSEQSQNYLANLISSEGRFSAKVSRFYMAEILLAIQYLHSKGFTYGFLSPKNMMIGDDGHIKLKYDFLNGIDCVVGDFEENIEYASIDYANSGEFGPVSDYWSMGIILYHMLGGITPFAAPGMDSVITRIKTNRIRFPEFFDEHARNLISKLLVTSPRDRLGYMEEDAEMIRKHPFFEGISWVDVLEKKIKPPFLFNVTKKECTSRSANLRILYTTDYLPDQKDGYGQTFRGYGSIYSPDIHRKIWRYGYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGH6</accession>
    <name>I7AGH6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">60S ribosome subunit biogenesis protein NIP7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_100540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with pre-ribosome complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NIP7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265367.1">
        <property type="nucleotide sequence ID" value="XM_009267092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGH6"/>
    <dbReference type="GeneID" id="20564484"/>
    <dbReference type="KEGG" id="ero:EROM_100540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100540"/>
    <dbReference type="HOGENOM" id="CLU_097217_0_0_1"/>
    <dbReference type="OrthoDB" id="27490at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042255">
        <property type="term" value="P:ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21146">
        <property type="entry name" value="Nip7_N_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055359">
        <property type="entry name" value="Nip7_N_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016686">
        <property type="entry name" value="Ribosomal_synth_fac_NIP7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017190">
        <property type="entry name" value="Rbsml_synth_fac_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="60S ribosome subunit biogenesis protein NIP7 pre-PUA" evidence="4">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0113" evidence="3">
        <location>
            <begin position="93"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR017190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="176" mass="19927" checksum="29E4894F84CE39D8" modified="2012-10-03" version="1">MRDLKQEEEKEVLKKLKFFIGDNVHRILSGEDKLFLHNQRVMLLSEKMRAATSMISRKNLIMAGTVIGRFTKGGSFRLGISSLNFLSQWAIHKIWIKNSAEMNYVYGNNSLKSHIFRSSEGIPINAGVFVFNQHDVPLGFGITALTPQGYSNAKGHALVLLRQSDTGEYLRNEEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGI8</accession>
    <name>I7AGI8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinetochore protein NDC80</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_100690</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
            <location evidence="10">Centromere</location>
            <location evidence="10">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the NDC80/HEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265382.1">
        <property type="nucleotide sequence ID" value="XM_009267107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGI8"/>
    <dbReference type="GeneID" id="20564499"/>
    <dbReference type="KEGG" id="ero:EROM_100690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100690"/>
    <dbReference type="HOGENOM" id="CLU_012583_1_0_1"/>
    <dbReference type="OrthoDB" id="7459479at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051315">
        <property type="term" value="P:attachment of mitotic spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.30">
        <property type="entry name" value="Ncd80 complex, Ncd80 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005550">
        <property type="entry name" value="Kinetochore_Ndc80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055260">
        <property type="entry name" value="Ndc80_CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038273">
        <property type="entry name" value="Ndc80_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10643:SF2">
        <property type="entry name" value="KINETOCHORE PROTEIN NDC80 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03801">
        <property type="entry name" value="Ndc80_HEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="9 10" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="7 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinetochore protein Ndc80 CH" evidence="12">
        <location>
            <begin position="37"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="209"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="453"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="544" mass="63103" checksum="03816945F4050771" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGJ8</accession>
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    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
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            <taxon>Encephalitozoon</taxon>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGJ8"/>
    <dbReference type="GeneID" id="20564509"/>
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    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100790"/>
    <dbReference type="HOGENOM" id="CLU_000022_45_4_1"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="108"/>
            <end position="511"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="673" mass="75785" checksum="61C3C6375286AE27" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGL8</accession>
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    <protein>
        <recommendedName>
            <fullName evidence="3">Anaphase-promoting complex subunit 1</fullName>
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        <name evidence="1" type="ordered locus">EROM_101050</name>
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    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Encephalitozoon</taxon>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265417.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGL8"/>
    <dbReference type="GeneID" id="20564535"/>
    <dbReference type="KEGG" id="ero:EROM_101050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101050"/>
    <dbReference type="HOGENOM" id="CLU_292731_0_0_1"/>
    <dbReference type="OrthoDB" id="26401at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="119894" checksum="D1A969931647F6BC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGZ5</accession>
    <name>I7AGZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Encephalitozoon</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009267297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGZ5"/>
    <dbReference type="GeneID" id="20564692"/>
    <dbReference type="KEGG" id="ero:EROM_110930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110930"/>
    <dbReference type="HOGENOM" id="CLU_2003898_0_0_1"/>
    <dbReference type="OrthoDB" id="10318957at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="124" mass="14582" checksum="569AB696BBEB5621" modified="2012-10-03" version="1">MLLLGNAGLAIFTLVIVQNLNDVFLHRARSPRGVEMKMKIQWIIFSIVTFFYGIFHLPLITSNMDKLFKMDMMALITVVIFTLQIIMLFIIYSMHPDLFGTKYNREVRSYIERLEAEHHEQSEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH12</accession>
    <name>I7AH12_ENCRO</name>
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        <recommendedName>
            <fullName evidence="1">Transcription factor TFIID subunit 8 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009267317.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH12"/>
    <dbReference type="GeneID" id="20564712"/>
    <dbReference type="KEGG" id="ero:EROM_111130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111130"/>
    <dbReference type="HOGENOM" id="CLU_130208_0_0_1"/>
    <dbReference type="OrthoDB" id="2193813at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08049">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019473">
        <property type="entry name" value="TFIID_su8_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10406">
        <property type="entry name" value="TAF8_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor TFIID subunit 8 C-terminal" evidence="1">
        <location>
            <begin position="104"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="170" mass="19789" checksum="693118323110D198" modified="2012-10-03" version="1">MRRILEDRLMRYLKEIGYEAVERKALEVFMASLEVYMLEYLKSVASVSRHGLKSHSTLLDILCFVEGKKFELPPFERVTYEKEEVEKEEKFVSPLSSGIEKYIHIYDFMPSFPPTHAFRQTIVKGGSRSSKSMNVKNRLEQSLRTEGNLLRLIKASGSLPPFVNFLYKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL83</accession>
    <name>I7AL83_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010700</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263911.1">
        <property type="nucleotide sequence ID" value="XM_009265636.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL83"/>
    <dbReference type="GeneID" id="20520697"/>
    <dbReference type="KEGG" id="ero:EROM_010700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010700"/>
    <dbReference type="HOGENOM" id="CLU_1652135_0_0_1"/>
    <dbReference type="OrthoDB" id="2192782at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="163" mass="18026" checksum="32BB669C2DF93DD7" modified="2012-10-03" version="1">MDSPDGRSGSSISLRKLGMIVAIFFFAMATGLMFIYRHLNNKDNRMSRPGPQGKFGVSLKRGGSESSPSGNERISLLSMDADHVKKLFNMFFEDIKNGREAYSDILRFLERHEKDPGLSSGTRGFIKTAISFLEGTSESKTGSSDIEVLMALVDVIAKRLPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALB4</accession>
    <name>I7ALB4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cdc48-like AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_011050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263946.1">
        <property type="nucleotide sequence ID" value="XM_009265671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALB4"/>
    <dbReference type="GeneID" id="20520732"/>
    <dbReference type="KEGG" id="ero:EROM_011050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011050"/>
    <dbReference type="HOGENOM" id="CLU_000688_12_0_1"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="6">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="241"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="514"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="780" mass="87539" checksum="D60D32F0ED8660A1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALC7</accession>
    <name>I7ALC7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATM1 mitochondrial ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_011250</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263966.1">
        <property type="nucleotide sequence ID" value="XM_009265691.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALC7"/>
    <dbReference type="GeneID" id="20520752"/>
    <dbReference type="KEGG" id="ero:EROM_011250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011250"/>
    <dbReference type="HOGENOM" id="CLU_000604_84_1_1"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF503">
        <property type="entry name" value="MITOCHONDRIAL POTASSIUM CHANNEL ATP-BINDING SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="9">
        <location>
            <begin position="169"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="379"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="606" mass="67505" checksum="5BB82D6F32484942" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALR1</accession>
    <name>I7ALR1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase</fullName>
            <ecNumber evidence="5">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_021340</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="5"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264106.1">
        <property type="nucleotide sequence ID" value="XM_009265831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALR1"/>
    <dbReference type="GeneID" id="20520896"/>
    <dbReference type="KEGG" id="ero:EROM_021340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021340"/>
    <dbReference type="HOGENOM" id="CLU_037460_3_1_1"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="268" mass="30320" checksum="F3B24D149A32BD27" modified="2012-10-03" version="1">MSPKESEKKEEVGAKRKIFVVVGMAGSGKTTFCQRLYSWISQDNCRVNPDTGLNSYIYSINLDPAVVNAKMPLNLDIRDVVDYHETMEKYNLGPNGGITTCLNLFLLNIGEYIDKIKEEYVIIDTPGQIEAFTWSSPGYMLIETLKTIGNVILVYVVDSVSSHKHAVFISNMMYAASLMCRYEVEALCLFNKKDLSRSEVLEEWISDYESFRDSLNEDNIFSPILGSMALYFEEFYNSVKSVSISSHTGSGRSDFFDAVNQLLEEKDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALZ4</accession>
    <name>I7ALZ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Calmodulin-dependent protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264171.1">
        <property type="nucleotide sequence ID" value="XM_009265896.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALZ4"/>
    <dbReference type="GeneID" id="20520964"/>
    <dbReference type="KEGG" id="ero:EROM_030530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030530"/>
    <dbReference type="HOGENOM" id="CLU_065009_0_0_1"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053235">
        <property type="entry name" value="Ser_Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24361">
        <property type="entry name" value="MITOGEN-ACTIVATED KINASE KINASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="390" mass="45725" checksum="9E9EF3B5F52CD1CB" modified="2012-10-03" version="1">MNKMKKNNWKVLGLGALSVMQGYHRRLSRKIQNIFFTTLVLLSFIFLYKKYFFVCRSKEDRRMEKMCLDFINKRVDKCLQHKSRYEQNKEFAAIKTHLPLIPLKMTRSNAVFLYTYRKPYIVIKRCIAPGTPGLEEDEIMKSLDHDHVVKFLKSFREKYVNLREEEETIIWIFMEHMDSKVSQDVVKGNENKIREIAKGILHGLKYLHGMGIAHLDLKISNVMGKETKDGIVYKIIDFGFARKLEHPSDNAEIQIPSKSFGTYPYKPPETSQLNMHGVKGDIWCLGAIVWFLSRRQTPFYFDDGRKNLEEWRMFIRGRLPISFKPNSSPALRDFVKKCMNFDRFARPNASDLLEHPFITGMPMKMASEDLNEMSEEFSSSSEYGSMESED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM88</accession>
    <name>I7AM88_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Heat shock transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_040350</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264301.1">
        <property type="nucleotide sequence ID" value="XM_009266026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM88"/>
    <dbReference type="GeneID" id="20521099"/>
    <dbReference type="KEGG" id="ero:EROM_040350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040350"/>
    <dbReference type="HOGENOM" id="CLU_030308_8_1_1"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="8">
        <location>
            <begin position="12"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="204"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="226"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="244" mass="28422" checksum="A247BC4D693B19A4" modified="2012-10-03" version="1">MEKPSTSFNFDKIPKFIMKLYKATSDEKYKGICWTSDGLKIHIYDRDVFVKETLPLISKTREFGTFIRMLNSYGFVKSKDIEEEDIYYNKNFRKGREDLLGIDDSLRMIKRKKSNDIRMRIGDGSLKEVVEYLYVQNQELYTELSACKERIERQERALNGLIEILSRVFRTNSQDFGARFKPNNLNLHNEMDLFLGELSGPLRDGYGPSASKPQEKSIPELSFKSGENHHTDSDSKNDGYDAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMF3</accession>
    <name>I7AMF3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_041180</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264381.1">
        <property type="nucleotide sequence ID" value="XM_009266106.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMF3"/>
    <dbReference type="GeneID" id="20521181"/>
    <dbReference type="KEGG" id="ero:EROM_041180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041180"/>
    <dbReference type="HOGENOM" id="CLU_049198_0_0_1"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="5"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="403" mass="44612" checksum="F628214BAB4A4C48" modified="2012-10-03" version="1">MKGGLSVKSAIVTMVTSMMGTGINYMPYAFKSVGYARGILLINGVGILTFFSLYAISIAANMSKDPSPTYSSLSTRISKYLKILVDLSIFSSCFGVNIVFYRYLTGLITVMFPMNRYVDPEVWRKIIVGVLAVPFLLLSLKKDLSSLKVTSYITVASVTYLAMLMGGYYLFIGERCVEEPARKFESQFSSGISCFMLALSCQANMVKTYTELEHKSPGNIIKVAMGASFLGTLIYGSVGLCGYFVFGNTIESSIIDILINPKAGINVYLMNSTIDKYALSSKIACIGAMLVLFGAFPVQMNPLAGIPLSYFIQEGRNTDLIRKKVVFVLMLMFLFLGMMEKLNIDTVLQMVGATAINLVSFTYPSIYYLYFRKRMDLMSILSIAVIIGSILSMIYMSYNIIYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMK8</accession>
    <name>I7AMK8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc-ribbon 15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264446.1">
        <property type="nucleotide sequence ID" value="XM_009266171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMK8"/>
    <dbReference type="GeneID" id="20521248"/>
    <dbReference type="KEGG" id="ero:EROM_050160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050160"/>
    <dbReference type="HOGENOM" id="CLU_164198_0_0_1"/>
    <dbReference type="OrthoDB" id="2186471at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031493">
        <property type="entry name" value="Zinc_ribbon_15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17032">
        <property type="entry name" value="Zn_ribbon_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon 15" evidence="1">
        <location>
            <begin position="34"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="123" mass="13703" checksum="765E4AC74FAF6822" modified="2012-10-03" version="1">MCCGTDLCCFFGCRSSAEKVDKPEGYPEPPKKFLCPYCGYLTDSEYRHDKYSFCICFIPCIPWGNSYPYLSCSHCKRNLGSIGDYRCQKCGVATTFESSNCPNCGEEKGHSGGSGGYRRLDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMS2</accession>
    <name>I7AMS2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Telomeric single stranded DNA binding POT1/Cdc13 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264536.1">
        <property type="nucleotide sequence ID" value="XM_009266261.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMS2"/>
    <dbReference type="GeneID" id="20521340"/>
    <dbReference type="KEGG" id="ero:EROM_051080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051080"/>
    <dbReference type="HOGENOM" id="CLU_057562_0_0_1"/>
    <dbReference type="OrthoDB" id="2186770at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011564">
        <property type="entry name" value="Telomer_end-bd_POT1/Cdc13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00976">
        <property type="entry name" value="Telo_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Telomeric single stranded DNA binding POT1/Cdc13" evidence="1">
        <location>
            <begin position="10"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="404" mass="46858" checksum="9B2A88975106B451" modified="2012-10-03" version="1">MQKEDNGEESTMISDMERNRYHTIYGVIVDWLEWKRCKGRDFMMTLDVIDESMKKLSVKVFSPKKIFSEGFCVGEVVRIGNIKLYDGFRAVTDRNNDVEVVFTPYKHLNGTVSPRAQQLMEFFQKNRKNLVKEREISEIEEGKYFDFNGELIDKQRERTNLVLLRLVDFSRNKLVQGYGGSSKYPKEMVLIVKAWGRFANEAEKCEIGGWYRIRNLKADEVGHNLYASLSESSKGSIIEMEKETTLGRYLGSKKDGYTRGFVCKEMNIETPKRMEKYSLGSIKSIDRPGVYRVRGWIKRYGPPGGMEVFLCRVCGSEDARIDGRCRCNGLETKGIKVLRLLLWDGEEEMVVICRNRLAEYVLDEESREQLGNRMFDCIILSVISKLGMIHHLIDSDFFEVKMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMV7</accession>
    <name>I7AMV7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264576.1">
        <property type="nucleotide sequence ID" value="XM_009266301.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMV7"/>
    <dbReference type="GeneID" id="20521381"/>
    <dbReference type="KEGG" id="ero:EROM_051490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051490"/>
    <dbReference type="HOGENOM" id="CLU_075601_0_0_1"/>
    <dbReference type="OrthoDB" id="2189698at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="318" mass="36628" checksum="67C14D565532B961" modified="2012-10-03" version="1">MDTRIKIDLDKEIYESGSEIKGAIDIKMRCPLCITKINLILSKAMKMKIQEIDRKEGSGEKFEKIYDGYRYEFEVYSNDDPLGEISSGHHKFPFRFLLRKEDEGSSEIKGVYFDVLCHIKSIYDLYAEIYVLGIHNPVYTVRKEIQVVDSITEPRDFHTTIDVISPICLLRKRYNIHFEVNKQFYYSGDYLTIEATIPSKNPKIKSMECFAYEILNVSVEGREILRTKYIGGGQAEHHAGGRVLRATLKIPSTTPSTVTGSEFSIRVVLFVNLELYRGTPVRVKKYIPVIKKNIVYPEIDCMDVLEGEVYPERILALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN14</accession>
    <name>I7AN14_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Disintegrin and metalloproteinase domain-containing protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_060290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable zinc protease.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264621.1">
        <property type="nucleotide sequence ID" value="XM_009266346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN14"/>
    <dbReference type="GeneID" id="20521426"/>
    <dbReference type="KEGG" id="ero:EROM_060290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060290"/>
    <dbReference type="HOGENOM" id="CLU_036314_0_0_1"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000003">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF159">
        <property type="entry name" value="ADAM METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13582">
        <property type="entry name" value="Reprolysin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="532"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="5">
        <location>
            <begin position="362"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00056552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00074021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="553" mass="62051" checksum="C138D2C6E1534C0D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN89</accession>
    <name>I7AN89_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264701.1">
        <property type="nucleotide sequence ID" value="XM_009266426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN89"/>
    <dbReference type="GeneID" id="20521508"/>
    <dbReference type="KEGG" id="ero:EROM_061120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061120"/>
    <dbReference type="HOGENOM" id="CLU_1209820_0_0_1"/>
    <dbReference type="OrthoDB" id="2191458at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="215" mass="25037" checksum="83BB27A6D3AC9C7A" modified="2012-10-03" version="1">MQKAIELVTSEIRSTHDSEAIERWYLSYLERKACPESMFGDVLSITMPDIESSDILKSTAMLFEASQKATDEMSLRSISHSYENSMEIISIFTIYNSIFPIIERHIKDKILGSKVVDVYNDASMHLNGPREGTQEILEGRRRFFFDFYIPVKIYLVCTGKSHEIKDKEKKFVMMYLAKKRGDGWCEAHTNQFYEEMSNGEEKVRKALEFLFSKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN93</accession>
    <name>I7AN93_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal protein L14E/L6E/L27E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264706.1">
        <property type="nucleotide sequence ID" value="XM_009266431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN93"/>
    <dbReference type="GeneID" id="20521513"/>
    <dbReference type="KEGG" id="ero:EROM_061170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061170"/>
    <dbReference type="HOGENOM" id="CLU_176430_0_0_1"/>
    <dbReference type="OrthoDB" id="2188806at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="CDD" id="cd23702">
        <property type="entry name" value="eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="106" mass="12151" checksum="652CEABF1142154E" modified="2012-10-03" version="1">MRTIKLGRIVTPALKEKRHTYGIIVGVIDATFVLIQRKDGEREMCSISNLHLEEESFDIKGLSAEEIGDLIPESTFTEDTTNDFDRFKLRLRKRVEESLLKEKGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANG7</accession>
    <name>I7ANG7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chromosome segregation ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_070630</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264811.1">
        <property type="nucleotide sequence ID" value="XM_009266536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANG7"/>
    <dbReference type="GeneID" id="20521622"/>
    <dbReference type="KEGG" id="ero:EROM_070630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070630"/>
    <dbReference type="HOGENOM" id="CLU_001042_4_1_1"/>
    <dbReference type="OrthoDB" id="5575062at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1060.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="Smc hinge domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="4">
        <location>
            <begin position="491"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="158"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="232"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="439"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="690"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="882"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1104" mass="128256" checksum="5E99754956687017" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANI4</accession>
    <name>I7ANI4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">General transcription and DNA repair factor IIH subunit TFB4</fullName>
            <shortName evidence="13">TFIIH subunit TFB4</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">RNA polymerase II transcription factor B subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_070830</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the 7-subunit TFIIH core complex composed of XPB/SSL2, XPD/RAD3, SSL1, TFB1, TFB2, TFB4 and TFB5, which is active in NER. The core complex associates with the 3-subunit CTD-kinase module TFIIK composed of CCL1, KIN28 and TFB3 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TFB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264831.1">
        <property type="nucleotide sequence ID" value="XM_009266556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANI4"/>
    <dbReference type="GeneID" id="20521642"/>
    <dbReference type="KEGG" id="ero:EROM_070830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070830"/>
    <dbReference type="HOGENOM" id="CLU_1294389_0_0_1"/>
    <dbReference type="OrthoDB" id="17307at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004600">
        <property type="entry name" value="TFIIH_Tfb4/GTF2H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12831">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIH TFIIH , POLYPEPTIDE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03850">
        <property type="entry name" value="Tfb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="8 13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="213" mass="24345" checksum="26F8A232E0654DD9" modified="2012-10-03" version="1">MILFLLIDLKNENWELTTRDLFNDIIVFLNAYHKSSRSNRFIVVSGKNILFDSSENDISEIYKITEFNHGYDVGDLGYALCLHKDEPTQIVIFTLGRERKDEYLRYLKCMFAAQRLGVRISAFSLFENKTILQCCVSTGGDYSTSEDRCLRFLLSLLGTGGSQKPLGFPATCYCHNRQILLGLVCPICLSVFCKFIPVCKKCKSKFSFAKYES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANK6</accession>
    <name>I7ANK6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_071080</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264856.1">
        <property type="nucleotide sequence ID" value="XM_009266581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANK6"/>
    <dbReference type="GeneID" id="20521668"/>
    <dbReference type="KEGG" id="ero:EROM_071080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071080"/>
    <dbReference type="HOGENOM" id="CLU_001103_15_0_1"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001975">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000296">
        <property type="entry name" value="ATP-dependent DNA helicase RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018982">
        <property type="entry name" value="RQC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09382">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00956">
        <property type="entry name" value="RQC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 11 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="245"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="449"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="731"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="737"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="765" mass="87708" checksum="A866EFD80F9BD1E0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANN3</accession>
    <name>I7ANN3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264886.1">
        <property type="nucleotide sequence ID" value="XM_009266611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANN3"/>
    <dbReference type="GeneID" id="20521698"/>
    <dbReference type="KEGG" id="ero:EROM_071380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071380"/>
    <dbReference type="HOGENOM" id="CLU_153332_0_0_1"/>
    <dbReference type="OrthoDB" id="2191937at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="139" mass="15960" checksum="0FBC4FCD9A019372" modified="2012-10-03" version="1">MCLFVHMHCFIFGNPRNNTYKVVGPYIGGMRDAMAIFRKGKKVYSIGPFDPLILDAVKGLEEMARNAYGDELLYIEVNGWMISSLSGIDDVFIILISKRIEMEKLKHAYESYRVCVAYENIKLMDVLLSMYSTKNADRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7APD3</accession>
    <name>I7APD3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">U3 small nucleolar RNA-associated protein 11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265181.1">
        <property type="nucleotide sequence ID" value="XM_009266906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APD3"/>
    <dbReference type="GeneID" id="20564291"/>
    <dbReference type="KEGG" id="ero:EROM_090670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090670"/>
    <dbReference type="HOGENOM" id="CLU_1635364_0_0_1"/>
    <dbReference type="OrthoDB" id="2192991at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007144">
        <property type="entry name" value="SSU_processome_Utp11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03998">
        <property type="entry name" value="Utp11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="24"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="19711" checksum="C0459438196C6DB3" modified="2012-10-03" version="1">MREKIYKERIQPKSRRKYGKLEKKKELVKRLSKINKQRDEIKKAREEIRNKSGQEYFFGYHSVRKDGRSVYRVDKPTLEELGKIKAYIDNEIQRSKMKLEKYIPIPSGTHIRFEEDGSEKDTEIVFGKNEEVRKEFERYMEELFMKRKEVLEKIDKLKDQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7APE5</accession>
    <name>I7APE5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-rich repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265196.1">
        <property type="nucleotide sequence ID" value="XM_009266921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APE5"/>
    <dbReference type="GeneID" id="20564307"/>
    <dbReference type="KEGG" id="ero:EROM_090830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090830"/>
    <dbReference type="HOGENOM" id="CLU_1517852_0_0_1"/>
    <dbReference type="OrthoDB" id="2191907at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF1">
        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="161" mass="18321" checksum="7FC3A7F9671A34D8" modified="2012-10-03" version="1">MDNLREAASIVRSVDIEGFCDLCNMDLELISPDVMEALKTNKLIKKLDLSSNKLKELPSDIGALNWLVELDLSNNEIGKIPEEMNDMKSLEVLNLSNNKLTSFPWKVLRLGRTGTLKSLDLRGNPLYRIYHTAFGRFFLRLFYGDIVKTGYDVEETTKCSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7APF2</accession>
    <name>I7APF2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L-type lectin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_090930</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265206.1">
        <property type="nucleotide sequence ID" value="XM_009266931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APF2"/>
    <dbReference type="GeneID" id="20564317"/>
    <dbReference type="KEGG" id="ero:EROM_090930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090930"/>
    <dbReference type="HOGENOM" id="CLU_1026828_0_0_1"/>
    <dbReference type="OrthoDB" id="270293at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051136">
        <property type="entry name" value="Intracellular_Lectin-GPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005052">
        <property type="entry name" value="Lectin_leg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223:SF28">
        <property type="entry name" value="LECTIN, MANNOSE BINDING 1 LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12223">
        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="7">
        <location>
            <begin position="38"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="292" mass="32546" checksum="BA96197A5D8EA2EE" modified="2012-10-03" version="1">MGRKLRVCLFLIGKFYKIKYPMFALLHILGCLCSERVFVQEVSLLSPYVDEIGRFLNWKTSGEQVIKAHGRDSFVQLGYSSPNSSGAILASHPIGSERFGIDIIIEIEEGEKGEGDEGMAIWISDEDVFSEGNCFGRSCNFKGLLVIIKPSGKSYIGVKAGDISINTSNIARNLDRVEYRNFPSGEKFVLRIEQKSYELSVYLGEPDNLSLVHSFRSNVVKEGDLLGVSAMTDKSSTSFRIVGIESYHLKSTGGVYTKEDVHSGGKLIWVLFAGVVFVTAYYLYNIQMKRDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7APL0</accession>
    <name>I7APL0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cullin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265296.1">
        <property type="nucleotide sequence ID" value="XM_009267021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APL0"/>
    <dbReference type="GeneID" id="20564408"/>
    <dbReference type="KEGG" id="ero:EROM_091840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091840"/>
    <dbReference type="HOGENOM" id="CLU_387335_0_0_1"/>
    <dbReference type="OrthoDB" id="2191622at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cullin N-terminal" evidence="1">
        <location>
            <begin position="19"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="713" mass="83246" checksum="7AD3D9FE831C7908" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7APR0</accession>
    <name>I7APR0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pre-mRNA-splicing factor 38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_100480</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PRP38 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265361.1">
        <property type="nucleotide sequence ID" value="XM_009267086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APR0"/>
    <dbReference type="GeneID" id="20564478"/>
    <dbReference type="KEGG" id="ero:EROM_100480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100480"/>
    <dbReference type="HOGENOM" id="CLU_1618990_0_0_1"/>
    <dbReference type="OrthoDB" id="190958at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005037">
        <property type="entry name" value="PRP38"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23142">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03371">
        <property type="entry name" value="PRP38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5 7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0747">Spliceosome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="164" mass="18917" checksum="D43668051D58946E" modified="2012-10-03" version="1">MQYKGINRMTREKILSSEEFKNMRSFTEPDVVGSICSLNSIGGLIKGIPHKFLCLIQKMGAISMKEEVVATSLEDLRPSEPGPLDSSMKVFRGNVYFIAASLMYLRLSKRFHEYKPLVRSFLMDFRKIPIIDGQNNKEFIYLDVFADDLLNKNRIFNVHLNRTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7APU3</accession>
    <name>I7APU3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265396.1">
        <property type="nucleotide sequence ID" value="XM_009267121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APU3"/>
    <dbReference type="GeneID" id="20564513"/>
    <dbReference type="KEGG" id="ero:EROM_100830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100830"/>
    <dbReference type="HOGENOM" id="CLU_105494_0_0_1"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="229" mass="26463" checksum="C2E83193423E45C4" modified="2012-10-03" version="1">MLLSTSLRSRHPNRTSSSVILNCFGYFCVSSCDEIWGREKSLSENIPMIFGEAGSCLLEDLKSSALKPYRHTEVKAIQGENEYLDAKMEGVRRLAGEGISEELSVNYVMMKYFKERNERILRSYKFHRSLSLFDDFFSAKKTSDMLSCEEEEYMRRCHDILGEYLEPFSHFDFTISTPPIQFFVQIITLEDCGVVMDEGDLIELKKDRIYFIKRSAVSHLIGNGLVRII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7APV8</accession>
    <name>I7APV8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DnaJ-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265411.1">
        <property type="nucleotide sequence ID" value="XM_009267136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APV8"/>
    <dbReference type="GeneID" id="20564528"/>
    <dbReference type="KEGG" id="ero:EROM_100980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100980"/>
    <dbReference type="HOGENOM" id="CLU_107282_0_0_1"/>
    <dbReference type="OrthoDB" id="2190572at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051948">
        <property type="entry name" value="Hsp70_co-chaperone_J-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44360:SF1">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="62"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="209" mass="24282" checksum="4B3C9ECA81F9F69D" modified="2012-10-03" version="1">MNYSEAARSCLSRKDYAGYLENAQRQYELTGSTEDAEEMEKARKAFSLHKEIEKFLKKSPSDYYDILGVPKDATQPQIKRAFNMLIMKFHPDRTGMHESSAVSGMIQNAYTTLGNPEKRRRYDMAREAPAFNMPTTGFSYSGDAMQNVFFNVFRTQGYQEPFMYSGDFYDHLYSQMYRRFNHRRPNPNDSDAQKVAAFLIVIILIFLAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7APW9</accession>
    <name>I7APW9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263854.1">
        <property type="nucleotide sequence ID" value="XM_009265579.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APW9"/>
    <dbReference type="GeneID" id="20520638"/>
    <dbReference type="KEGG" id="ero:EROM_010120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010120"/>
    <dbReference type="HOGENOM" id="CLU_122489_0_0_1"/>
    <dbReference type="OrthoDB" id="1711508at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="160" mass="18559" checksum="FA666261B5CBC1A3" modified="2012-10-03" version="1">MGIEPDMKFKSYLVYNRPNLDLLIKFLKTHDTSYVLWTTGMRRNATNLINHLSHVGFNEYLGWYSQLDCKAGKVKLDSECNLWVKDLNVVARNHNVDVERCILVDDSIDKSVHTQNFICCPEYIPGTEDDGIKYICKHLDDFFECKEECIAKKLVYGEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7APZ0</accession>
    <name>I7APZ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265441.1">
        <property type="nucleotide sequence ID" value="XM_009267166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APZ0"/>
    <dbReference type="GeneID" id="20564559"/>
    <dbReference type="KEGG" id="ero:EROM_101290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101290"/>
    <dbReference type="HOGENOM" id="CLU_034494_0_0_1"/>
    <dbReference type="OrthoDB" id="10022113at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="561" mass="62983" checksum="4AC1E576DBF35753" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ45</accession>
    <name>I7AQ45_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_110150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265496.1">
        <property type="nucleotide sequence ID" value="XM_009267221.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ45"/>
    <dbReference type="GeneID" id="20564614"/>
    <dbReference type="KEGG" id="ero:EROM_110150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110150"/>
    <dbReference type="HOGENOM" id="CLU_1057793_0_0_1"/>
    <dbReference type="OrthoDB" id="2017974at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="4"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="263" mass="30574" checksum="27B4B00FCE31C178" modified="2012-10-03" version="1">MLRISIVPDTNVFISHLDIVKKLYEDEFPVDVIMSISRVVLRELDYNKGKMMEAREAIRFLEKVYNAPITELEGRLKDSSMDVVDCAKLIPNIKNNDDEILNFASKQIHPVILTGDKAFYLKSKCYNVESVLVHNLPYEDLKLKILKIYTGVEPMDIDEDGSKPGEDERIKEQIRDRLFPVVLAIMERGIGKPYVLFFPNDLKMITLDFLLNLIIKENYLFRPYLPRRSKEIVQNLKEKIKDAHGKELHALGSELLMLFRVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ57</accession>
    <name>I7AQ57_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dynamin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_011030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263944.1">
        <property type="nucleotide sequence ID" value="XM_009265669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ57"/>
    <dbReference type="GeneID" id="20520730"/>
    <dbReference type="KEGG" id="ero:EROM_011030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011030"/>
    <dbReference type="HOGENOM" id="CLU_008964_5_0_1"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003130">
        <property type="entry name" value="GED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020850">
        <property type="entry name" value="GED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02212">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00410">
        <property type="entry name" value="G_DYNAMIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51388">
        <property type="entry name" value="GED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="5">
        <location>
            <begin position="25"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="GED" evidence="4">
        <location>
            <begin position="511"/>
            <end position="593"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="593" mass="66797" checksum="BDA5DE35AC472B0C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQL5</accession>
    <name>I7AQL5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111630</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265641.1">
        <property type="nucleotide sequence ID" value="XM_009267366.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQL5"/>
    <dbReference type="GeneID" id="20564762"/>
    <dbReference type="KEGG" id="ero:EROM_111630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111630"/>
    <dbReference type="HOGENOM" id="CLU_049198_0_0_1"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="7">
        <location>
            <begin position="5"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="402" mass="44350" checksum="6E52B23AD9CD2D22" modified="2012-10-03" version="1">MIKGLSNKDAVVAMVLSMVGTGITFMPYAFKSAGYLNAIFLMIFFAMGTAVSCYCISYAAKYSGHENPTYSSIASSISTTLGYIVNVSIFFNGYLAAVNFYRYLSDLIVGNSYYIQNLTSDYEKSRKVVVLILAVPFLYLSSKKTLSGLALTTYLSIASVLYLAFLMAYLFFTVGSTFAIGEVKPFNESLGESVPFFISSMVCQANMVKIYAEMENKSNRNIMIVSLGAATGGMLVNCLIGLSGYFVFGDGVSGEIITELSDMNNPVNKHLRSGWDKKNIMSGMAVYGMMLTLFGGYPIQVNPVVDMIFKNLPVEKRTEVNRKGIVILLFLVAIFLNLMQELKTKVIKKFLGATFSNSVAFVYPFVYYLSIKKEKSLLSTSVCIFMIGMSVMVSLYTIFKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR23</accession>
    <name>I7AR23_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AN1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_040230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264289.1">
        <property type="nucleotide sequence ID" value="XM_009266014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR23"/>
    <dbReference type="GeneID" id="20521087"/>
    <dbReference type="KEGG" id="ero:EROM_040230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040230"/>
    <dbReference type="HOGENOM" id="CLU_057016_6_1_1"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1110.10">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035896">
        <property type="entry name" value="AN1-like_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050652">
        <property type="entry name" value="AN1_A20_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000058">
        <property type="entry name" value="Znf_AN1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10634">
        <property type="entry name" value="AN1-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01428">
        <property type="entry name" value="zf-AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00154">
        <property type="entry name" value="ZnF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118310">
        <property type="entry name" value="AN1-like Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51039">
        <property type="entry name" value="ZF_AN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="AN1-type" evidence="6">
        <location>
            <begin position="69"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="19"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="137" mass="15855" checksum="236E8A310765A45E" modified="2012-10-03" version="1">MDYLGPMKMEDFVRRMEEEIRSEDDVKKEDPGRREHISEPQIHKKRKIAIPPCSLQKKKHIADNNTVEKKESGVMSCGKCGKRLRLTNNYSCRCGNTYCIMHRFHDQHGCTFDYKTMAIAKLSAQNPKIVGKKIGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARA0</accession>
    <name>I7ARA0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264389.1">
        <property type="nucleotide sequence ID" value="XM_009266114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARA0"/>
    <dbReference type="GeneID" id="20521189"/>
    <dbReference type="KEGG" id="ero:EROM_041260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041260"/>
    <dbReference type="HOGENOM" id="CLU_103891_0_0_1"/>
    <dbReference type="OrthoDB" id="2191343at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="226" mass="26006" checksum="A45607700EB5D3B8" modified="2012-10-03" version="1">MKSISGCPMRFESLKVFFKIVRNESYFKIQIITLESLDIFEAALREEKIDYSKHFSSMIKKSESLSLILRDSDICLPGPGKYVLFSNTRHREFVQIVFIPTLEEKLSIVGDKYIVQAYKYKNINELLKIASKEESRIESHFGSGLDYFESVIMLVVSSKSKFKEILSSSREMDSKLGNSFFLQMKLNGLVDKLLVVRNGDRYRVNTSKSVLRHIGKRIGYDADSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARD6</accession>
    <name>I7ARD6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="ero:EROM_041670"/>
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    <dbReference type="HOGENOM" id="CLU_1461298_0_0_1"/>
    <dbReference type="OrthoDB" id="2193761at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="185" mass="22094" checksum="39AD4DA463586B56" modified="2012-10-03" version="1">MVDHIHRKFNKSFILLYVGNYCIKIDSFSYLFLKGFVPFYIEIYKFYCFFLAIYIENRWYALMIFDATLESFEEASLRVQKTLTSLKELIYKADVCKEKIPKDISFPKMDFDINKYLTGICSVEDEIERELKELVVFLDIQNEEVEKDVAYYKEMVSRAGIRTKLENISYLVNKKMYLDEGKGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <name evidence="10" type="ordered locus">EROM_070510</name>
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    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASA2"/>
    <dbReference type="GeneID" id="20521610"/>
    <dbReference type="KEGG" id="ero:EROM_070510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070510"/>
    <dbReference type="HOGENOM" id="CLU_000604_57_6_1"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
        <property type="entry name" value="ABC TRANSPORTER G FAMILY MEMBER 28"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041:SF139">
        <property type="entry name" value="PROTEIN SCARLET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="384"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="405"/>
            <end position="431"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="437"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="473"/>
            <end position="490"/>
        </location>
    </feature>
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        <location>
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            <end position="571"/>
        </location>
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            <begin position="6"/>
            <end position="247"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="583" mass="65436" checksum="38580490F39F80A8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASB0</accession>
    <name>I7ASB0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070610</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="GeneID" id="20521620"/>
    <dbReference type="KEGG" id="ero:EROM_070610"/>
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    <dbReference type="HOGENOM" id="CLU_148322_4_3_1"/>
    <dbReference type="OrthoDB" id="442921at2759"/>
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        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="CDD" id="cd01763">
        <property type="entry name" value="Ubl_SUMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="25"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="103" mass="11165" checksum="1E01C8898224432F" modified="2012-10-03" version="1">MGSSDSGNESKTNEGTLQATASKKMPLKLVDQDGTMLVFNVKVNTTFKKILDAFSRNVGKNSSEFRLLFNGKNIDPGKTPNDFGFEGNEELEVVTSQVGGSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASI6</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">EROM_071460</name>
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    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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            <taxon>Eukaryota</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
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                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264894.1">
        <property type="nucleotide sequence ID" value="XM_009266619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASI6"/>
    <dbReference type="GeneID" id="20521710"/>
    <dbReference type="KEGG" id="ero:EROM_071460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071460"/>
    <dbReference type="HOGENOM" id="CLU_526783_0_0_1"/>
    <dbReference type="OrthoDB" id="2196336at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="237"/>
            <end position="471"/>
        </location>
    </feature>
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        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="316"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="350"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="525" mass="56035" checksum="B3E57A2B0C7D0179" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATE0</accession>
    <name>I7ATE0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265194.1">
        <property type="nucleotide sequence ID" value="XM_009266919.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATE0"/>
    <dbReference type="GeneID" id="20564305"/>
    <dbReference type="KEGG" id="ero:EROM_090810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090810"/>
    <dbReference type="HOGENOM" id="CLU_2589741_0_0_1"/>
    <dbReference type="OrthoDB" id="2195340at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="80" mass="9698" checksum="1D2B0AB0B32ED0F3" modified="2012-10-03" version="1">MRFRIENIKEIGISRAFNGVGMEEAHLILEFLGHRIFDYENKRLDERRTWRFNEDSTEHGKDRDEAVERILNIHKSLRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATE9</accession>
    <name>I7ATE9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5097 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090960</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265209.1">
        <property type="nucleotide sequence ID" value="XM_009266934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATE9"/>
    <dbReference type="GeneID" id="20564320"/>
    <dbReference type="KEGG" id="ero:EROM_090960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090960"/>
    <dbReference type="HOGENOM" id="CLU_553237_0_0_1"/>
    <dbReference type="OrthoDB" id="2196404at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031511">
        <property type="entry name" value="DUF5097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17020">
        <property type="entry name" value="DUF5097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5097" evidence="2">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="507" mass="58391" checksum="83A386AE92E9A93B" modified="2012-10-03" version="1">MIESFAIKKFIINYFIEHSQEDIDKMVLNIHRHLLRGFLMNEVVYNMKSGEYGRISRVGGARYGVVIQSKDGGHEKDCHFSDLARRDEVSASGVKGYLLGITMETPFGRVLKENAFGSIKDSSGVMKCKVPQNQCYEGTRPLRDSQKSLDREKTQGMWPSAENAGDRGAMERQDDKANAVEVEKNGLTEDIDIDSILKLRDDQILRIPGLERPDIESTMKILGVLVGFRSFFDIERIDVKELAGVIIDPLYESDIVCKTHERLIEMLKAEIDNIGMDVFIENSKPCIALVFECVCSSKDKKQDDLESIEEPRQVLEKKQWKADLEKFIKETSSQAGIDISHIVPCIFAKDRPLSKEEIRSRLVLLELLLNMFFTTEPFREVVSEKMETSKELERRKRELQARLRKPILALDSEASLDTDKRSEVKKELIDTMEKIFQLPTNIRIGEIDGIRFLNLNRKIYAAKERDYYLMSRDALLRLCKKYNDRSKSKRNVLVSIEQYMEILQNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATM7</accession>
    <name>I7ATM7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Serine-threonine kinase receptor-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_100090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the WD repeat STRAP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265324.1">
        <property type="nucleotide sequence ID" value="XM_009267049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATM7"/>
    <dbReference type="GeneID" id="20564439"/>
    <dbReference type="KEGG" id="ero:EROM_100090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100090"/>
    <dbReference type="HOGENOM" id="CLU_043845_0_1_1"/>
    <dbReference type="OrthoDB" id="24966at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027525">
        <property type="entry name" value="eIF3i"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19877:SF1">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24805">
        <property type="entry name" value="EIF3I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="328" mass="35809" checksum="5AEB04AA6C96EF68" modified="2012-10-03" version="1">MDDRLPVEGEGYFLNIKCHERPITEVKFNHDGDLIFTAGKDSIATLLRDDGSVIGEYKGHRGSIFSLAIDNKSDALMTGSADQSVILWDVPTGKMRAQIDIGAVVKGLDFYRDGTLCLACSDETMGKTPLIGILDTRSGNFKKVFMPTSSPVKILLDISENFLVYCDSKGGIISVDLRNSQVIKSVNAHTSKINNIRPSRCRTFFITGSTDSQSKIIDFLDLSVQKTFVCEEPVNCASIFNTNDKVICVGGINARDVTTTKGKSSFDANFFDVVTCEKIGSYSTHFGTINSVDVHPSGRLYCSGGEEGTVSILAFGQDFFKANFTTFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATN9</accession>
    <name>I7ATN9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265339.1">
        <property type="nucleotide sequence ID" value="XM_009267064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATN9"/>
    <dbReference type="GeneID" id="20564455"/>
    <dbReference type="KEGG" id="ero:EROM_100250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100250"/>
    <dbReference type="HOGENOM" id="CLU_152058_0_0_1"/>
    <dbReference type="OrthoDB" id="2194943at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="142" mass="16360" checksum="625467768AC21C65" modified="2012-10-03" version="1">MGPKTNTRSIKSLRRLTRSRTQVIFFDGTQIMKSDEGMRPHLPGCKDDTEEEVPSSRSIPKTCNILNGEERMFSFKDLLETFNSLEGGMTQKTSRLESNTGPIESLNRDTMMKRKEMFDHPSNDVFKRKGNMDSLSKKYTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU34</accession>
    <name>I7AU34_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265489.1">
        <property type="nucleotide sequence ID" value="XM_009267214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU34"/>
    <dbReference type="GeneID" id="20564607"/>
    <dbReference type="KEGG" id="ero:EROM_110080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110080"/>
    <dbReference type="HOGENOM" id="CLU_1602702_0_0_1"/>
    <dbReference type="OrthoDB" id="2192056at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18197" checksum="EA3E5EEB6C13F053" modified="2012-10-03" version="1">MIVRAINYEDLYIDSDFKDIGDLKTKIALKYGIPTDSQCIVFNGKFYVLGIFDSPLETFSDTYGNPLGPFGCPDAVFNYLESNGMSIDKIRAHVFPHKEDPKEKSPTLSRIIGVKPAVKKVIEGRYEDISKISEGLKEEEKAAVIENPGEYIDLILTQVGESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUM1</accession>
    <name>I7AUM1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265664.1">
        <property type="nucleotide sequence ID" value="XM_009267389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUM1"/>
    <dbReference type="GeneID" id="20564785"/>
    <dbReference type="KEGG" id="ero:EROM_111860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111860"/>
    <dbReference type="HOGENOM" id="CLU_994078_0_0_1"/>
    <dbReference type="OrthoDB" id="10256122at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042627">
        <property type="entry name" value="FBXW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436">
        <property type="entry name" value="F-BOX/WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44436:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="9"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="92"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="132"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="280" mass="31709" checksum="2FAB4C6391FD4F17" modified="2012-10-03" version="1">MKYRRTAILKIHNSWIKSLDIDPYDAFLASSSMDGTTGIVDIETKQLLITIPLKGVCEDAKFSKTQPYIFCALLDGLIRSYDLVDREFIREYYGHTSSVLCLDTYDKRIFSGSSDCTVRAWDVRSRNSVSVMKGHRLPVTHIMFRNGLIYSSSMDGNVFLWDERSTKTPIVGFASSVTSMCMCDETLFVSVGKKVFECKDIPYEVGLKSNALSLESYNNNYYLIGSEKYVFMKSKEINDPDYQFGIEGPGNIVKVTASKERIIVAGTSKNIEFLEKDEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGP6</accession>
    <name>I6ZGP6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Helicase DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_010130</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263855.1">
        <property type="nucleotide sequence ID" value="XM_009265580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGP6"/>
    <dbReference type="GeneID" id="20520639"/>
    <dbReference type="KEGG" id="ero:EROM_010130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010130"/>
    <dbReference type="HOGENOM" id="CLU_000315_8_5_1"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016197">
        <property type="entry name" value="Chromo-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000953">
        <property type="entry name" value="Chromo/chromo_shadow_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023780">
        <property type="entry name" value="Chromo_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF11">
        <property type="entry name" value="KISMET, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00385">
        <property type="entry name" value="Chromo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00298">
        <property type="entry name" value="CHROMO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54160">
        <property type="entry name" value="Chromo domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50013">
        <property type="entry name" value="CHROMO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Chromo" evidence="8">
        <location>
            <begin position="212"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="310"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="648"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1245" mass="144847" checksum="C2F5E79A8B683E42" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHH1</accession>
    <name>I6ZHH1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264120.1">
        <property type="nucleotide sequence ID" value="XM_009265845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHH1"/>
    <dbReference type="GeneID" id="20520911"/>
    <dbReference type="KEGG" id="ero:EROM_030040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030040"/>
    <dbReference type="HOGENOM" id="CLU_507157_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012468">
        <property type="entry name" value="DUF1686"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07937">
        <property type="entry name" value="DUF1686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="474"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="508"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="537" mass="61422" checksum="6CD79355B691F3E8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHL3</accession>
    <name>I6ZHL3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="1">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="1"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264165.1">
        <property type="nucleotide sequence ID" value="XM_009265890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHL3"/>
    <dbReference type="GeneID" id="20520958"/>
    <dbReference type="KEGG" id="ero:EROM_030470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030470"/>
    <dbReference type="HOGENOM" id="CLU_324173_0_0_1"/>
    <dbReference type="OrthoDB" id="289038at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF644">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="2">
        <location>
            <begin position="111"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="934" mass="108135" checksum="6CEACD72917A88B7" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHU9</accession>
    <name>I6ZHU9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Endoplasmic reticulum membrane-associated oxidoreductin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_040130</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264280.1">
        <property type="nucleotide sequence ID" value="XM_009266005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHU9"/>
    <dbReference type="GeneID" id="20521077"/>
    <dbReference type="KEGG" id="ero:EROM_040130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040130"/>
    <dbReference type="HOGENOM" id="CLU_065181_0_0_1"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 17" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707083" evidence="17">
        <location>
            <begin position="18"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="284"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN82783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="325" mass="37706" checksum="86253D83B055B318" modified="2012-10-03" version="1">MIWTSAMLLATIMASISDRVDLSISKINSTTYRNLEKLVSKDSYSLVRTKDLGEFHSKSKCTLLSCLITKKSIFNEEYINLLEIREAYTGFKTGDGSAEIWRRIWEISNEDPLLPILVSGLQFSILTHLSAFHKKFFGTYLPNPTLFQKRFQDKHRLNFYLTYLLVRNCVGNITIDEQEMDEGLSAVIQTIKFQGSTNWVTQSVDLEKTIQRVEEMARLLKHISCEKCQLWGTIQLKGLRAALRVFSGSTNLERLERFFLINLFMRLSVSVKENIRLRRYRAPFLATVALYWMEILSFATSLLMIFLVSKIRNKFKSRITLKSCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHV2</accession>
    <name>I6ZHV2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Histone acetyltransferase complex SAGA/ADA subunit ADA2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_040190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264285.1">
        <property type="nucleotide sequence ID" value="XM_009266010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHV2"/>
    <dbReference type="GeneID" id="20521083"/>
    <dbReference type="KEGG" id="ero:EROM_040190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040190"/>
    <dbReference type="HOGENOM" id="CLU_018273_0_0_1"/>
    <dbReference type="OrthoDB" id="270417at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02335">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041983">
        <property type="entry name" value="ADA2-like_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055141">
        <property type="entry name" value="TADA2A_B-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374:SF20">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTER 2-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12374">
        <property type="entry name" value="TRANSCRIPTIONAL ADAPTOR 2 ADA2 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22941">
        <property type="entry name" value="TADA2A-like_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25299">
        <property type="entry name" value="ZZ_ADA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Transcriptional adapter 2-alpha/beta-like" evidence="4">
        <location>
            <begin position="139"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="343" mass="40456" checksum="9CEFE85F943B92AB" modified="2012-10-03" version="1">MAFSCSNELKEVSILCDNCFVEMTTLTYIKCSECEIDLCLSCFVNQVETSLHSKYHKYRVISKMDTRIHEEKWTLMEEILFIEGLSVSGIGNWSGVSKYVGGERDVEDHFYKVFGFQKEVGGLPRITEKSSNPYRGTISSYMPYRKDFDIEYMNGYEALIKDLSLHEDEGKLEKRMIEAVLDSYIKIIRYRNRRKHAILGRNLIDMEGLMKKNEQFKVVDNIKWIVPYLTKPDFNTLLYGIYIEKKLHELLNKRKQKNSGMTDPDGILSSKYFVGERERELCETHGLSPDVYLGLKREMAYYLAKKKRFTKEDFNRLFGFLKETDTLYNLFLEYGWIHKEKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHY3</accession>
    <name>I6ZHY3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA (guanine(37)-N1)-methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">tRNA methyltransferase 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TRM5</name>
        <name evidence="12" type="ordered locus">EROM_040550</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">Predominantly in the mitochondria and in the nucleus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRM5 / TYW2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="10"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264320.1">
        <property type="nucleotide sequence ID" value="XM_009266045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHY3"/>
    <dbReference type="GeneID" id="20521119"/>
    <dbReference type="KEGG" id="ero:EROM_040550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040550"/>
    <dbReference type="HOGENOM" id="CLU_022610_2_0_1"/>
    <dbReference type="OrthoDB" id="408788at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070901">
        <property type="term" value="P:mitochondrial tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03152">
        <property type="entry name" value="TRM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025792">
        <property type="entry name" value="tRNA_Gua_MeTrfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 10 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="11">
        <location>
            <begin position="110"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="235"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="360" mass="41521" checksum="9B08F772B3F65086" modified="2012-10-03" version="1">MNFKNEMKSVICLKALGTSDKEASECIQRVKESIRKVPRIPSVIKVKKRYTTFRILTDTGYDLLILVNEELSNEESELIDVEIELLYEYFTYNEVLRKVLPKEVQTPSSFEIVGSIVHLNLDEEQIKYKDIIGQVVYDKTGKTVITKTGQISNEYRSFDLEVIGGEGILETIHKEGDILFYIDYKNVYWCSKLQNERLDLVQKLRDGDVLCDPFCGVGPVSLAALKKGCKVYSNDLNPHAISCLRKSMEINKLDPRNIEVFNLPASEFLEKIAGRKIDHFFLNLPDHSLDYLRKISAWDDDSLVHCYFFCRNNEDVGQYIFSRVGLRVDPSMVKIVRKVSPSKCMYKLEIPCIFLRSGFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIW2</accession>
    <name>I6ZIW2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="ero:EROM_060340"/>
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    <dbReference type="OrthoDB" id="10260897at2759"/>
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    </dbReference>
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        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:GTP binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="184"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome biogenesis protein BMS1/TSR1 C-terminal" evidence="4">
        <location>
            <begin position="330"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="657"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="743"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
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            <begin position="278"/>
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        </location>
    </feature>
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        <location>
            <begin position="326"/>
            <end position="342"/>
        </location>
    </feature>
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        <location>
            <begin position="665"/>
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        </location>
    </feature>
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            <end position="767"/>
        </location>
    </feature>
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        <location>
            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="777" mass="89892" checksum="8EE13D09E258D86E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJB3</accession>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_070770</name>
    </gene>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
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        <lineage>
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            <taxon>Microsporidia</taxon>
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            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN83328.1"/>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:ribosomal large subunit assembly"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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            <end position="189"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN83328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="277" mass="31068" checksum="FB877781B691811F" modified="2012-10-03" version="1">MAKREKFFHPRMTKMDGKERKELTYEKAKKLFGTYSRFALVGIENVESTQLKDIKRQWGNNVEFLMGKNSAIKRAIADLGKPELSRVLDLIKGDVCFVFFKGDARDIKKTIDENVREACAKVGNIAQKDVWVDSCITGMTPDKTSYFQVLGIATKITKGKVEIISPYKVLSKGDKVGPSQANLLGMLNIKPFSYKMTMHHVYEDGVVYDSSLIDIEKEDILSTLRSAISSIAAISLGADIITQASMPYNIRNSFKDILQISLGIDFMIKEQSMITAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJQ0</accession>
    <name>I6ZJQ0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264970.1">
        <property type="nucleotide sequence ID" value="XM_009266695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJQ0"/>
    <dbReference type="GeneID" id="20521789"/>
    <dbReference type="KEGG" id="ero:EROM_080530"/>
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    <dbReference type="HOGENOM" id="CLU_1503424_0_0_1"/>
    <dbReference type="OrthoDB" id="2190586at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="20578" checksum="435EDB1ABE468876" modified="2012-10-03" version="1">MIKNGRFGYNSLTPKLMVITGVLIAVVLCMIMFPSPREIAVEAEVSNDEFQTKTYLQASRKMKEELMIKKEDFEGFYFTLRLINPARDRVVIIVNGTKIKTIVSSKYIIRFIETIENRDKSPFYFIFLSYLMGSLYFPVGTINFVSNSEGVWYNNVCYRGHPAIFRRICLLLVRDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJY9</accession>
    <name>I6ZJY9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ser/Thr protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_081620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265075.1">
        <property type="nucleotide sequence ID" value="XM_009266800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJY9"/>
    <dbReference type="GeneID" id="20521897"/>
    <dbReference type="KEGG" id="ero:EROM_081620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081620"/>
    <dbReference type="HOGENOM" id="CLU_000288_25_2_1"/>
    <dbReference type="OrthoDB" id="5337378at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050339">
        <property type="entry name" value="CC_SR_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11042:SF190">
        <property type="entry name" value="MITOSIS INHIBITOR PROTEIN KINASE MIK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="77"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="362" mass="42146" checksum="F51476D5659FA2DE" modified="2012-10-03" version="1">MSHLKKIYMLKREREEAIFSSPKTPSKKIPYKDIFISSVPFAADTRDSSLINEGSTLDDVFTCTDNVASGPDKESMCNFVCKTGEGAFYEVFVNRKDGRREAGHILEEPVMLYKSRKLCVYDADYTVVKKSKKRISGELDRKSRMREIEMLKRVGSDHVVRYYDFWESRGYLFIELEYCNIGTLRDYIHEVYFLRKSKFSPEITRKMMYELAKGLEAIHSCNIVHLDLKPENILMKSIVSKEKCSGRCQCMFPTDPGSFVFKISDFNISRYEGEDIDEDGDKRYMAPEVLRDVCTKASDIYSLGLIYLEIIAEIILPKSGDNWVRLRRNDFRGVKLDRVCKLMLDMNYKKRLNAKEIIDIFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK79</accession>
    <name>I6ZK79_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Histone H3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_090460</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265160.1">
        <property type="nucleotide sequence ID" value="XM_009266885.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK79"/>
    <dbReference type="GeneID" id="20521985"/>
    <dbReference type="KEGG" id="ero:EROM_090460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090460"/>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000085">
        <property type="entry name" value="Histone H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45810">
        <property type="entry name" value="HISTONE H3.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="9">
        <location>
            <begin position="44"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="140" mass="15663" checksum="5B62ADC3A81B2EF2" modified="2012-10-03" version="1">MSRTKQTASKALGGKAPRKGVSIKSIPSSGSSSVMAKRTRFKAGALALKEIRKYQKSTDLLIRKRPFQRMVRELCKGGEGMRFQASAIVAFQEAVENFLTNLMEDAYRCVLHAKRVTLMPKDICLVYKIKYANILYAALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK97</accession>
    <name>I6ZK97_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Replication factor C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_090710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265185.1">
        <property type="nucleotide sequence ID" value="XM_009266910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK97"/>
    <dbReference type="GeneID" id="20564295"/>
    <dbReference type="KEGG" id="ero:EROM_090710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090710"/>
    <dbReference type="HOGENOM" id="CLU_042324_0_1_1"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF5">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25361">
        <property type="entry name" value="AAA_lid_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="34"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="309" mass="35045" checksum="EA10697515D77E3F" modified="2012-10-03" version="1">MDLLVNKYRPNGVQDIVGNHTTIELIALIIESRDMPHLLLSGPPGTGKTTCAKILARKLLPNKEGLLELNASDERGIDTVRTTIKSFAQRRIKDCGFKIIILDEADSMTATAQQAMRRVMEIHSSECRFILICNTLTKIFEPIQSRCAILMFERIDHSTILGRLREISNDEGIKINREALDLIVELSDGDMRQSLNILQACINSSETIDQNRIVKIIGLPSPKRIEKVLEKLMENNVEEALEMFDKIWDEKFDPLDLIGSFFKAAKNMENYDLLKVIGLANLRISEGVNSRLQFYGMFHDILNMGSKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZRZ2</accession>
    <name>I6ZRZ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">U3 small nucleolar ribonucleoprotein component Mpp10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_010110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MPP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263853.1">
        <property type="nucleotide sequence ID" value="XM_009265578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZRZ2"/>
    <dbReference type="GeneID" id="20520637"/>
    <dbReference type="KEGG" id="ero:EROM_010110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010110"/>
    <dbReference type="HOGENOM" id="CLU_1643691_0_0_1"/>
    <dbReference type="OrthoDB" id="445326at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005732">
        <property type="term" value="C:sno(s)RNA-containing ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012173">
        <property type="entry name" value="Mpp10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17039:SF0">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN MPP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04006">
        <property type="entry name" value="Mpp10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="161" mass="18573" checksum="9F234CB2920D8FC8" modified="2012-10-03" version="1">MNESIEEIEKLLVKPKEWKYAGEVDIKKRPKNSLLEREEIDFKQGPPLIPFSSKQDSEVESITLQRIREGTFDNHEYNIKEVVEIVDEVPDTENPECKDIISLYNEIEGDLMQIVDFGNNGFVPDCEIRIVQGKEDGSGPRKKLDVKSLDRIRNVTVLKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS20</accession>
    <name>I6ZS20_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_010470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263888.1">
        <property type="nucleotide sequence ID" value="XM_009265613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS20"/>
    <dbReference type="GeneID" id="20520673"/>
    <dbReference type="KEGG" id="ero:EROM_010470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010470"/>
    <dbReference type="HOGENOM" id="CLU_130531_0_0_1"/>
    <dbReference type="OrthoDB" id="2190163at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097237">
        <property type="term" value="P:cellular response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="157" mass="17334" checksum="C4E5366B1BE50295" modified="2012-10-03" version="1">MRNVVILGQGPGAYMCGIYAHTANLSPIIIKACKKSMTDFSGSEKVVGVKGIQSPEELVGLVERQSKNMGIEVREEEILNISKKQNHFEIETNTGTHETRSLVIDSKDLEKKYRPLLGGRGVFYVSDKAPYREAIILASAGCKVSFDVKEFIDAINQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSG9</accession>
    <name>I6ZSG9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Protein disulfide isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_020760</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probable disulfide isomerase, which participates in the folding of proteins containing disulfide bonds. May act as a dithiol oxidase. Acts as a regulator of endoplasmic reticulum-mitochondria contact sites via its ability to regulate redox signals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264048.1">
        <property type="nucleotide sequence ID" value="XM_009265773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSG9"/>
    <dbReference type="GeneID" id="20520836"/>
    <dbReference type="KEGG" id="ero:EROM_020760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020760"/>
    <dbReference type="HOGENOM" id="CLU_042417_0_0_1"/>
    <dbReference type="OrthoDB" id="427280at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052250">
        <property type="entry name" value="PDI_TMX3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46426:SF1">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE TMX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="499" mass="56961" checksum="51FB43A3EE2E4FD4" modified="2012-10-03" version="1">MMLLLYLLGSFAEIMEQCKPIDEGYALTKYFTQWCPSCQSIAPVIEEISNKIDRHGANLKIRSADCDKCSCPNIKSYPTLELSKDGEVLGRLEGGHGYDAIVEFIMRHTGIEKSVFDGHSAQKEATVRKLTKSDFLSGFDGPYVVLFYSREDDKYREIFKELAKIYDGKLNIGEIDSAESAEFVNRYDIRSYPSISGIFNGLVVPFIEKEREPSVLSLIEFCDKLIEPSFSNLTFEKFNDLASNLEPGEPIYVVFHKNVALANAYFQRMAHMYKFRARIYRSDDQALFEKASIFPKALGTGSDASTSPNHEDVVVLSVYKNGIFYRYTDTFGDEVKITDWIFHTHYKYLTKVDNRNFYSIFHGLKPVMILLTRGEEFVGKMNEFSTDRHLGVPYTDMIFATMEIDEYPLFIPTLLPKLSTPALIVFEPWANLFRHKKVKLTEDNFISAAMDTYALYQKNSLPLYPPNSRSLLKYSVFGVLALGIGLYCSSKTSFAKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSH4</accession>
    <name>I6ZSH4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L35A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_020810</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264053.1">
        <property type="nucleotide sequence ID" value="XM_009265778.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSH4"/>
    <dbReference type="GeneID" id="20520841"/>
    <dbReference type="KEGG" id="ero:EROM_020810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020810"/>
    <dbReference type="HOGENOM" id="CLU_100745_3_0_1"/>
    <dbReference type="OrthoDB" id="1166329at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="113" mass="12716" checksum="03021AF0FCD69028" modified="2012-10-03" version="1">MPEAIMSEEVLSRTTIPATFVSFKRGQRRLHPRYALLKINNVASKDEASKYIGNGVICYKTSNKGKVYPVHGVVTRIHGNSGAVRARFARNLDPKMLGTHVFIKLYKVELDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSU1</accession>
    <name>I6ZSU1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">LSM complex subunit LSM4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LSM4</name>
        <name evidence="10" type="ordered locus">EROM_030260</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Binds specifically to the 3'-terminal U-tract of U6 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">LSm subunits form a heteromer with a doughnut shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264143.1">
        <property type="nucleotide sequence ID" value="XM_009265868.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSU1"/>
    <dbReference type="GeneID" id="20520936"/>
    <dbReference type="KEGG" id="ero:EROM_030260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030260"/>
    <dbReference type="HOGENOM" id="CLU_099537_3_3_1"/>
    <dbReference type="OrthoDB" id="747253at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097525">
        <property type="term" value="C:spliceosomal snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01723">
        <property type="entry name" value="LSm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034101">
        <property type="entry name" value="Lsm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="Sm" evidence="9">
        <location>
            <begin position="5"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="87" mass="10007" checksum="2A45FEDCF88099AC" modified="2012-10-03" version="1">MYPLTLLRISRNKIVELELKNNDHLRGLLYKCDMAMNLHLKSVTIQKEDGSSIFINECYLKGTSIKLVKLESKILLEQKEEAVSNKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSY7</accession>
    <name>I6ZSY7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264203.1">
        <property type="nucleotide sequence ID" value="XM_009265928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSY7"/>
    <dbReference type="GeneID" id="20520996"/>
    <dbReference type="KEGG" id="ero:EROM_030850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030850"/>
    <dbReference type="HOGENOM" id="CLU_569898_0_0_1"/>
    <dbReference type="OrthoDB" id="2194089at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="452"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="487" mass="55763" checksum="0BF9E5B94D6F3203" modified="2012-10-03" version="1">MREAGNGDLPSENQDKEKRRRLATQLTEEMRLIRREFESTKDMGNGARFLYDFEKRLPFRHREIRYASIPNKKAEKRAVNESDRIRMAAEYAAEAGVLMVMDVLRQSRKVVFSKDWEVGYRENNVINILQKIEKKRRKKGLLKCCEGGRIDKRDVENTWKMMMDAEKKRMDVLNLISHEISLTKRSRLICINIPFWFSSETEGQHPESDCVFGKEGSEGGLTYCRTKISKMYPNESVSYENVIFPLVRDDSPPTIFNTRMDDSPEKTERTPPKAQMIERYKKYLKSFARINAKQSSRSNFNIAQPSAASHPTSQHVFKKIPIEYLRCKNAKDIINHKYKHVVVDRVAHMARRAETKYSLGDLEDGLSGDLCSSGSDDTSIRYTFLIEAALGKTSDSGKKDIPNGDIKKVSGCWKDETQGYIPEMPLQVPRREGGEVASDANGQASLGNSSNKKVEDETPEEPHRNSIGRATLGFKYDMGNSRDEENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT21</accession>
    <name>I6ZT21_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Histone H3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_031340</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264253.1">
        <property type="nucleotide sequence ID" value="XM_009265978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT21"/>
    <dbReference type="GeneID" id="20521048"/>
    <dbReference type="KEGG" id="ero:EROM_031340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031340"/>
    <dbReference type="HOGENOM" id="CLU_078295_4_1_1"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000096">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="13">
        <location>
            <begin position="45"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="144" mass="15937" checksum="6AF1FA2F76917E63" modified="2012-10-03" version="1">MARTKQSARKTTGGKAPRKQLSAKSARKAVSPASSGGAKKSRYRPGSVALKEIRRYQKSTDFLIRRLPFQRACRSVVKECSNATDIRFQGPALASIQEALEVYLVGLFEDAMLCACHAKRVTVFPKDISLVLKLRSRHVKSISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT79</accession>
    <name>I6ZT79_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL22</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">60S ribosomal protein L22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_040680</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264333.1">
        <property type="nucleotide sequence ID" value="XM_009266058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT79"/>
    <dbReference type="GeneID" id="20521132"/>
    <dbReference type="KEGG" id="ero:EROM_040680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040680"/>
    <dbReference type="HOGENOM" id="CLU_2145832_0_0_1"/>
    <dbReference type="OrthoDB" id="10259820at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002671">
        <property type="entry name" value="Ribosomal_eL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038526">
        <property type="entry name" value="Ribosomal_eL22_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10064:SF0">
        <property type="entry name" value="FI24544P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01776">
        <property type="entry name" value="Ribosomal_L22e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00041214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="112" mass="12425" checksum="3C55B8AEAD27A209" modified="2012-10-03" version="1">MATASEKTTRKFTVDCTKPASDSLISPSDLGAFLQQKIKCNMGKKEKLLQINVNGNIVEISVTGGFISKQGLKWQTGRFLHMKKLRAFIKIFAQGPDGFELGYINVEEGKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU88</accession>
    <name>I6ZU88_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polyprenol reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264718.1">
        <property type="nucleotide sequence ID" value="XM_009266443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU88"/>
    <dbReference type="GeneID" id="20521527"/>
    <dbReference type="KEGG" id="ero:EROM_061310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061310"/>
    <dbReference type="HOGENOM" id="CLU_116809_0_0_1"/>
    <dbReference type="OrthoDB" id="2189450at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22168" checksum="855788A90946B559" modified="2012-10-03" version="1">MFVLIETLYFALLPVVTVASHIFINDLTRHGHIPEGMTKNNYQYFYAYGVILSLLLPVKNIYPFHLGRRFIETKVLKYSDRSKMNLLQFIHGLVYYTFVCMHLRDKAISNKGVFMLLNALQSVSHYFVFIRKTAGYSHYVVEVVIYAFIYCEVGTIQMLFNLLYVLSFVLSTIRNRRILREKPRENIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUG1</accession>
    <name>I6ZUG1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translocation protein SEC62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_070650</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264813.1">
        <property type="nucleotide sequence ID" value="XM_009266538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUG1"/>
    <dbReference type="GeneID" id="20521624"/>
    <dbReference type="KEGG" id="ero:EROM_070650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070650"/>
    <dbReference type="HOGENOM" id="CLU_040936_1_0_1"/>
    <dbReference type="OrthoDB" id="200187at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031204">
        <property type="term" value="P:post-translational protein targeting to membrane, translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004728">
        <property type="entry name" value="Sec62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12443:SF9">
        <property type="entry name" value="TRANSLOCATION PROTEIN SEC62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03839">
        <property type="entry name" value="Sec62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="23230" checksum="3243643FA2B7C7C0" modified="2012-10-03" version="1">MGVELKKYEDILRWVNAEEKTLRYRRRVGVFKGSDALVLLTKKGLDSSQARSVMQELLNESILFKVSINKRNIRECDVVLDQRFQEDHHYVFANEKTSNISLVLCGVFVLVTFTIVLFRMWPRNIQQRLFSYMVYPLGGFITFIIVLAIIRLILFSITYILYSPGIWLFPNLFEDVGFFESFVPLWEYHGSKKVKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUG3</accession>
    <name>I6ZUG3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Histone acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_070700</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264818.1">
        <property type="nucleotide sequence ID" value="XM_009266543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUG3"/>
    <dbReference type="GeneID" id="20521629"/>
    <dbReference type="KEGG" id="ero:EROM_070700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070700"/>
    <dbReference type="HOGENOM" id="CLU_020445_3_1_1"/>
    <dbReference type="OrthoDB" id="427795at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022052">
        <property type="entry name" value="Histone-bd_RBBP4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050459">
        <property type="entry name" value="WD_repeat_RBAP46/RBAP48/MSI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22850">
        <property type="entry name" value="WD40 REPEAT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12265">
        <property type="entry name" value="CAF1C_H4-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Histone-binding protein RBBP4-like N-terminal" evidence="7">
        <location>
            <begin position="13"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="201"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="245"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="289"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="346"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="384" mass="43409" checksum="3B83136B30FAD66B" modified="2012-10-03" version="1">MNSQVLEQKVINEEYKVWKKNVPYLYDLMFSHTLQWPSLSVQWFPDVRRDEDIGRTTQRLLLSTHTSGSEDEYIMIVNVEFPDEFDESLNEEVNGDMRLKIVQRISVMDEANRVRYNPSACNILAVRSDISDVHIYDYTKHLSHEKIPRPDMVLRGHESGGFGLSWNNLSPGEVASCGEDGNVCVFDITQESSLVSPMVTLSRHKAAVNDCSFGFFDKELLSSVGDDGILMFWDTRTGDCIHLVEEAHSSDVLSVSFSSLDGNVVATSSEDKSVKIWDRRNLSQPFQVFLGHSKDVLNVEWSPHDSGVLASGSADRRVIVWDMNRVGEPVSEEYKAEGPSEMRFLHGGHTSTVCDISWNPAEPFEIASVSEDNILQIWQMPQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUN9</accession>
    <name>I6ZUN9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264923.1">
        <property type="nucleotide sequence ID" value="XM_009266648.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUN9"/>
    <dbReference type="GeneID" id="20521739"/>
    <dbReference type="KEGG" id="ero:EROM_080030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080030"/>
    <dbReference type="HOGENOM" id="CLU_037459_0_0_1"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19821">
        <property type="entry name" value="Bbox1_BBX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19756">
        <property type="entry name" value="Bbox2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049808">
        <property type="entry name" value="CONSTANS-like_Bbox1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00643">
        <property type="entry name" value="zf-B_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="B box-type" evidence="5">
        <location>
            <begin position="154"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="5">
        <location>
            <begin position="206"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="257"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="430" mass="49955" checksum="6920CB0965CE38AF" modified="2012-10-03" version="1">MHDIEYLLKYLLDSTTLKIERLQDITNLQQAAAFEKKFGDTKRIKAFCTEIGSTTYFPVFGVPLSQDSITHKVKLCSVAVGESIYVSKEYVKQMNPPGHYNSFITSDLGYQVGFLAESQTDVRNYWYAIKDPSKILPLFEITFEYDAELEMRSKGSFICDRCRISQSTSFCPAERASFCKECDEEVHNDHFLKRHVRYYFNEVGKKKFIYCTSHMDTMVDYFCEECFIPVCTKCKISGNHSELPNGSHNLLNYVEACDKLQDLLKAGELRLVEEEKRLKENIGDFKKNVEVFQNNISDVRGKIESEYRNSMNELHAFEKREFQVINTHYISYLIRLAEIERMREYPSGLEPSQLLRTFKSVTIQRESLSEDTEEDVFKPAELSLKGHLTLKDEENYTTRKGYSPRMEKSTQLYVETRGINVNSHGKVNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUR0</accession>
    <name>I6ZUR0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L26</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264948.1">
        <property type="nucleotide sequence ID" value="XM_009266673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUR0"/>
    <dbReference type="GeneID" id="20521765"/>
    <dbReference type="KEGG" id="ero:EROM_080290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080290"/>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_1"/>
    <dbReference type="OrthoDB" id="1688503at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="142" mass="16495" checksum="AEDAE0C1C3AE1016" modified="2012-10-03" version="1">MKNLRKQKTSSRRKQRKAHFACNDGEKRIRSSARLSRELRKKYGFRTFPLRTGDTVVVKGGKFDGKEGTISKINYRDYKVYVEGCFVTKNNGTNALVPIYPSRLTITNFFLENGREQQLERKKAAREKNMEILRSKQEATAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV21</accession>
    <name>I6ZV21_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C-CAP/cofactor C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_081800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TBCC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265093.1">
        <property type="nucleotide sequence ID" value="XM_009266818.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV21"/>
    <dbReference type="GeneID" id="20521915"/>
    <dbReference type="KEGG" id="ero:EROM_081800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081800"/>
    <dbReference type="HOGENOM" id="CLU_1384153_0_0_1"/>
    <dbReference type="OrthoDB" id="194775at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="2">
        <location>
            <begin position="71"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="199" mass="23046" checksum="33C0ED555004AEE1" modified="2012-10-03" version="1">MDEDAIRKIKEGDFEGARQTILNIEGCIREEPSLYNRKVLLEKVTRLKDIYNTYNVVETPIIPFKNHTLFKKQKNETDGIKNKRYVKDVNNSIVTIEDCTEVVIENCHNTVFPHFSCEKSVVLNNIKDCRVSCSGHQIRMNGCQNVELEVYTLTGVFLQSSKGIVVKKYGTKENNKFEDVYDFSSPFESRNYTIFPNQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVA6</accession>
    <name>I6ZVA6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265153.1">
        <property type="nucleotide sequence ID" value="XM_009266878.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVA6"/>
    <dbReference type="GeneID" id="20521977"/>
    <dbReference type="KEGG" id="ero:EROM_090390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090390"/>
    <dbReference type="HOGENOM" id="CLU_181948_2_0_1"/>
    <dbReference type="OrthoDB" id="6332053at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="52" mass="6407" checksum="8501D0CFA11A8EB6" modified="2012-10-03" version="1">MGSRKTALVKTRLMKALKKNKEIPAWKRMMKEHKGEFNRARRYWRAKKLKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVC1</accession>
    <name>I6ZVC1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Post-GPI attachment to proteins factor 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_090540</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the lipid remodeling steps of GPI-anchor maturation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PGAP3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265168.1">
        <property type="nucleotide sequence ID" value="XM_009266893.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVC1"/>
    <dbReference type="GeneID" id="20564278"/>
    <dbReference type="KEGG" id="ero:EROM_090540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090540"/>
    <dbReference type="HOGENOM" id="CLU_032917_2_1_1"/>
    <dbReference type="OrthoDB" id="419770at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007217">
        <property type="entry name" value="Per1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148">
        <property type="entry name" value="PER1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13148:SF0">
        <property type="entry name" value="POST-GPI ATTACHMENT TO PROTEINS FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04080">
        <property type="entry name" value="Per1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="2 7" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="274" mass="32398" checksum="4C6A7A0060B6DC27" modified="2012-10-03" version="1">MKYCFCKCFEESKDFVKTGLIDWIFNRDVDEKTSSMCHLLCLKLTNNGNIKRNGRWGFQPVLGMTEFFSALFSFINLITNILCFHKMLKKHLRVTRLGKLYYIQYYICNLAFIASTLFHIHENSFTRNCDYFLAFLTILFGFYMAFVRVILIGSPSLERAIRRPLQCIFILFYAYHIHRMSNIEFDYVYNKVSCAIIITLTLLSHLVTFLKYRKMGHSKNILLFTFFFFLAGAIEIQDVPPYAYLIDSHAIWHLISCISTPFYLLFWSGDVYMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVT7</accession>
    <name>I6ZVT7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265358.1">
        <property type="nucleotide sequence ID" value="XM_009267083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVT7"/>
    <dbReference type="GeneID" id="20564475"/>
    <dbReference type="KEGG" id="ero:EROM_100450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100450"/>
    <dbReference type="HOGENOM" id="CLU_078273_0_0_1"/>
    <dbReference type="OrthoDB" id="287041at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="1">
        <location>
            <begin position="75"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="311" mass="37055" checksum="7CD0589AB619424F" modified="2012-10-03" version="1">MSLEKFLQAFKSRMEERRFPYITSSILSFGVGVAILGRPNGFKDQSIIGYIRRAKEKICRFAKERDMSIPTLPPQEQARPTIVFDCEDFLVKKKFSFFSFDFLVTKRSFTDLFLFHSAHIYELVHVSESSVSFSRYLLHRIDPYGCISYKVYCKDKKMFSSKHLNRPLEKFVVISTREGEYHRDFNENILRLEKWQGEPEWGLLGLLNFLHNLHFIGVKDFRDTLKTYFGKNFYNAYERVQKSIFVQRNLFTWDVEKKYRDRVNEINKKRQKDFEKAKRVMDMDLQDRGVKEEEEGSILGYVFSLCIKAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVV2</accession>
    <name>I6ZVV2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA replication factor C small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265378.1">
        <property type="nucleotide sequence ID" value="XM_009267103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVV2"/>
    <dbReference type="GeneID" id="20564495"/>
    <dbReference type="KEGG" id="ero:EROM_100650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100650"/>
    <dbReference type="HOGENOM" id="CLU_042324_5_0_1"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="354" mass="40550" checksum="D9603EAB05665498" modified="2012-10-03" version="1">MIWIEKYRPKSFDSIVGREKISSVLKSYTLETIPHLILHGGPGHGKKTTLLCLVTHLYGGSPEMKIRTVEVLSGAKKIEVSYMESNEYVEISPSQYGHHDKAVIQSIIKEMGQTKPILSMLKSAKKAPIRLVAITSAEELTLEAQAALRRTIEMYSSVLRVVLVCNELSRLIEPVRSRCFFLRIPGFSNEDIISNMCRISEKENYAIPKEKLEEICKESEGNMRRALCILELFCFNMIDKETKRPKINVKDLKLDWELMVMGISSIVKSNQTPEGLVEIRKSLYMLLNSCISPRTILIELLRSLILGEDFKTFLLLSHQALKYEERIRFGMKSIYHLEGFITSSMCVFNEKRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVV5</accession>
    <name>I6ZVV5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA 2'-O-methyltransferase fibrillarin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Histone-glutamine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_100700</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-glutaminyl-[histone H2A] + S-adenosyl-L-methionine = N(5)-methyl-L-glutaminyl-[histone H2A] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50904"/>
            <dbReference type="Rhea" id="RHEA-COMP:12837"/>
            <dbReference type="Rhea" id="RHEA-COMP:12839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265383.1">
        <property type="nucleotide sequence ID" value="XM_009267108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVV5"/>
    <dbReference type="GeneID" id="20564500"/>
    <dbReference type="KEGG" id="ero:EROM_100700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100700"/>
    <dbReference type="HOGENOM" id="CLU_059055_1_0_1"/>
    <dbReference type="OrthoDB" id="1859733at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000386">
        <property type="entry name" value="Fibrillarin, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="284" mass="31392" checksum="F63AF1525D193B68" modified="2012-10-03" version="1">MKNVNKKPSRGKIQKGGRPSKGDRNEGRGRMNNKKKGSVKAGLDKKILIEPHKRFPGVYVGRGKEDLLLTRSLVPGTSVYGERRISVEVEGEKVEYRVWNVYRSKLAAGIVCGVENIHIGPGSKVLYLGASSGTTVSHVSDIVGKDGMVYAVEFSERSGRDLINMSMKRANIVPIIEDARHPSRYRMLVPIVDCIFSDVSQPDQTRIVALNAQYFLKEGGGVDVSIKANCVNSSIPAETVFADEVNILRKSNIKPREQVTLEPFEKDHAMIVGKFVLAKSKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWB2</accession>
    <name>I6ZWB2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ethanolamine-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CTP:phosphoethanolamine cytidylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_110740</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.14" evidence="10"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265553.1">
        <property type="nucleotide sequence ID" value="XM_009267278.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWB2"/>
    <dbReference type="GeneID" id="20564673"/>
    <dbReference type="KEGG" id="ero:EROM_110740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110740"/>
    <dbReference type="HOGENOM" id="CLU_031246_1_0_1"/>
    <dbReference type="OrthoDB" id="40021at2759"/>
    <dbReference type="UniPathway" id="UPA00558">
        <property type="reaction ID" value="UER00742"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004306">
        <property type="term" value="F:ethanolamine-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006646">
        <property type="term" value="P:phosphatidylethanolamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044608">
        <property type="entry name" value="Ect1/PCYT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45780:SF2">
        <property type="entry name" value="ETHANOLAMINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="10"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Cytidyltransferase-like" evidence="12">
        <location>
            <begin position="182"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN84056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="320" mass="36844" checksum="493E023AD6CF2BCC" modified="2012-10-03" version="1">MASYEIRKVWADGCFDMFHYGHANALRQSKGLGDYLIAGVHSSLSINQEKGLPVMEDDERYEVVQGCRYVDEIVRDAPFVTQMDMIREYGVSVVAHGNDIILDSNGQDSYFQVRKTGMFKEVERTIGISTTETVGRMMLKNRGSCPDGEHGEYNKNSRYHDELFDLFKSSMKREKKGKVAFVDGNFDLFHAGHVVSLRIAREMGDYLIVGIHDDETTKEYTRNYPVLSTKERILTLMSCRYVDEVVVSPYLIGNEFIEKHGISIVGPSFDTKDLSRYDKIKEMVKHSYAENKFNYLSAEHIVNRIISNYQDYANRQKKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWD3</accession>
    <name>I6ZWD3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110940</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265573.1">
        <property type="nucleotide sequence ID" value="XM_009267298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWD3"/>
    <dbReference type="GeneID" id="20564693"/>
    <dbReference type="KEGG" id="ero:EROM_110940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110940"/>
    <dbReference type="HOGENOM" id="CLU_3406374_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="30" mass="3386" checksum="CC1A68E1F851B5BB" modified="2012-10-03" version="1">MFILYFSSLMPKSSARVLIKAIWASNAVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADD6</accession>
    <name>I7ADD6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome biogenesis protein BMS1/TSR1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_021350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264107.1">
        <property type="nucleotide sequence ID" value="XM_009265832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADD6"/>
    <dbReference type="GeneID" id="20520897"/>
    <dbReference type="KEGG" id="ero:EROM_021350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021350"/>
    <dbReference type="HOGENOM" id="CLU_031890_0_0_1"/>
    <dbReference type="OrthoDB" id="119302at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome biogenesis protein BMS1/TSR1 C-terminal" evidence="2">
        <location>
            <begin position="268"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="544" mass="62658" checksum="26AEC9FAD858CD46" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADN2</accession>
    <name>I7ADN2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264172.1">
        <property type="nucleotide sequence ID" value="XM_009265897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADN2"/>
    <dbReference type="GeneID" id="20520965"/>
    <dbReference type="KEGG" id="ero:EROM_030540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030540"/>
    <dbReference type="HOGENOM" id="CLU_169142_0_0_1"/>
    <dbReference type="OrthoDB" id="2190306at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="12355" checksum="BEB47E2A4C256B98" modified="2012-10-03" version="1">MEESEELRYLMRLVPEYMRIGGEGYILHRMNRAVNEDSWGKAMFCGRCLHLYIPVVNSRIRYDGVLTIECLGCGGKYVFNRTSGEFWKDSYEDKEAKTLEYYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADP3</accession>
    <name>I7ADP3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ER-Golgi tethering complex TRAPP subunit protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030690</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264187.1">
        <property type="nucleotide sequence ID" value="XM_009265912.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADP3"/>
    <dbReference type="GeneID" id="20520980"/>
    <dbReference type="KEGG" id="ero:EROM_030690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030690"/>
    <dbReference type="HOGENOM" id="CLU_1960235_0_0_1"/>
    <dbReference type="OrthoDB" id="2191898at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="129" mass="14725" checksum="15252BF76AE7DE02" modified="2012-10-03" version="1">MGELLVIISEKNDIVYQRMYSRASNEEYCRLIILIYGSIDILIWKMASTSSNYFDCLEKHGEMRISAYIMPSGYKLLFIHTRKDVRPFLEGIHRMFAPVLVSSSLSEDILGAQALDSGVDEAYRACFSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE74</accession>
    <name>I7AE74_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0328 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264432.1">
        <property type="nucleotide sequence ID" value="XM_009266157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE74"/>
    <dbReference type="GeneID" id="20521234"/>
    <dbReference type="KEGG" id="ero:EROM_050010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050010"/>
    <dbReference type="HOGENOM" id="CLU_059413_0_0_1"/>
    <dbReference type="OrthoDB" id="2198933at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019081">
        <property type="entry name" value="UPF0328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09591">
        <property type="entry name" value="DUF2463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="231" mass="25951" checksum="710FF28B54011766" modified="2012-10-03" version="1">MNPTSHLAHTQIRHFAGIVSIVFPAIMHFTLGPSIKDSLLLKFITVLLPFSYSAAQHFLLFRINRSLDYKSSSLLHSILYFLLNLFLFVFAVIDGISTVTFTLDKWDDDVSFSILLPSFVVPSTYLLSTACILTPGSIQFPDTGFNFLIDILILLRIVVGIMFPLHNSSYIYIAVPSFILILLRSLNLGYSSSSVTEYAVWRQLIVFLIFGISLFIHTIIACMSISICINI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEE5</accession>
    <name>I7AEE5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_051040</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264532.1">
        <property type="nucleotide sequence ID" value="XM_009266257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEE5"/>
    <dbReference type="GeneID" id="20521336"/>
    <dbReference type="KEGG" id="ero:EROM_051040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051040"/>
    <dbReference type="HOGENOM" id="CLU_787757_0_0_1"/>
    <dbReference type="OrthoDB" id="2161379at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051972">
        <property type="entry name" value="Glutamate-rich_WD_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45903:SF1">
        <property type="entry name" value="GLUTAMATE-RICH WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="255"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="340" mass="38323" checksum="11E77C94684329D9" modified="2012-10-03" version="1">MAVHQSTEDSEEEISGLSSEECEVYEPQADDEELEVSEDAYNVMEYISLDWPAQSIDIFRGFTVVLGTNPPEGITPGMISIDLLPTNGMKMPGKMKISKRNVKESYNKVRVNGETIYCLSDNKFVVHNSKFDEVYVREINQGLGYGLCFGSTGCVFGTRSGEVSMNDWNFNEISMISVHEDSIEGLCYQDNMIFSGSCDHSIGITDIRAKKSVFQKKFEYDINAVDFNGDNMLAFGDDNGVIRLMDIRNYSVEEISRHKTPISCLKWRDGDILASGSDEQVCIWDISLDPGEEDGYLLFVHQGQKFYKDITFCRGDNNYVITTSIDGLCVFYPISFDPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEX2</accession>
    <name>I7AEX2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264702.1">
        <property type="nucleotide sequence ID" value="XM_009266427.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEX2"/>
    <dbReference type="GeneID" id="20521509"/>
    <dbReference type="KEGG" id="ero:EROM_061130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061130"/>
    <dbReference type="HOGENOM" id="CLU_891441_0_0_1"/>
    <dbReference type="OrthoDB" id="2193321at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="322" mass="37040" checksum="273188D0D46A0367" modified="2012-10-03" version="1">MIFCPMTMEVNSKDEESLTSFKRNLRKMILLEEEGRRRRESQSTRKLVSIRVGKIEGIMKEPGRKIVKEKRRVFEGPKMEYEQEGKVKERIKGFDSRGMAMEEKALEEEGTKDFVCLEEKGSNIQEEILEERVTENKSIKDERSRIVEECKSSVEKEFCEVGPKYKDECVDEECFKEISVEDIKKSLLAELDEDKESDMDIINTVVKSENPMQPAQEVIYVDNESTPQEVEKSPYGVGCNIYSYDTHYKISDLSKGNLGILSPSDEKGPCTHTSSTSDHAKELRDGHSEAEIVTYQDPEEERNERGWFSSIFGSIFSICCIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF49</accession>
    <name>I7AF49_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">26S proteasome regulatory complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264802.1">
        <property type="nucleotide sequence ID" value="XM_009266527.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF49"/>
    <dbReference type="GeneID" id="20521613"/>
    <dbReference type="KEGG" id="ero:EROM_070540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070540"/>
    <dbReference type="HOGENOM" id="CLU_029573_1_0_1"/>
    <dbReference type="OrthoDB" id="1418352at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050871">
        <property type="entry name" value="26S_Proteasome/COP9_Components"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040773">
        <property type="entry name" value="Rpn6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10678">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 11/COP9 SIGNALOSOME COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18055">
        <property type="entry name" value="RPN6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="181"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="389" mass="44837" checksum="2955D06BFA316415" modified="2012-10-03" version="1">MIDELKNIVGSKEATLEEKEEAVLEIFRVLISKGDYHEIGCVIMSLKGVWEDITTPRITKIIRRLFEMIPYSQDNFEHVLKLLTVLVEWADKENKKMLRLDLECKRIYALLKTEKYSEALAQIGPVAHELKKYDDKINLITLYVYESKAFYEIKSISKSKSSLTSARVLAVSAYCPPQLQAQIDLLSGIYICDERNYGVASSYFIEALEGFTLGKMPSEACVSLRYLILSKIMAGKREEINTAMKNKNTFKHLDDEVIKVLLRVSDSFGNRDLRSYSDVLQRYAEQLRCDSFVWSHLQYLYDVLLDRNIIKIIEPYSVVRINFIADVLGFEADIIEKKLRKMILDKVVDGTLDHIDECLILHGERPDNFFADECMKQAKALDCIVSSRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF69</accession>
    <name>I7AF69_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase subunit 8-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264827.1">
        <property type="nucleotide sequence ID" value="XM_009266552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF69"/>
    <dbReference type="GeneID" id="20521638"/>
    <dbReference type="KEGG" id="ero:EROM_070790"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070790"/>
    <dbReference type="HOGENOM" id="CLU_129987_0_0_1"/>
    <dbReference type="OrthoDB" id="20018at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="121" mass="13608" checksum="75E5293EDD4844C3" modified="2012-10-03" version="1">MQILADNFKITDIDKEGKLYTKVSRIYMQSPTISIALDYNTTLCKLQMGHAVEVRIFRGIHENIDCFYLAYGRIYSVEPRDNRVSITASFGGLLLIMDAPQEAVKGVGDKDDISLALTFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFK2</accession>
    <name>I7AFK2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265007.1">
        <property type="nucleotide sequence ID" value="XM_009266732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFK2"/>
    <dbReference type="GeneID" id="20521829"/>
    <dbReference type="KEGG" id="ero:EROM_080940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080940"/>
    <dbReference type="HOGENOM" id="CLU_165613_0_0_1"/>
    <dbReference type="OrthoDB" id="2192175at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="121" mass="14196" checksum="596049242F3FFEEB" modified="2012-10-03" version="1">MKPSTSSKTMQSTFQYITKTSLQNGIIRQREESLNMRCERPDETGFDRPGNKENYNPHIKEFTPTKTKQKKRYTALKEITERSKRPAQEASRASQEDETEMDLLFSSFPRSPFKSKNIREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFM1</accession>
    <name>I7AFM1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_081190</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265032.1">
        <property type="nucleotide sequence ID" value="XM_009266757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFM1"/>
    <dbReference type="GeneID" id="20521854"/>
    <dbReference type="KEGG" id="ero:EROM_081190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081190"/>
    <dbReference type="HOGENOM" id="CLU_314968_0_0_1"/>
    <dbReference type="OrthoDB" id="538223at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050505">
        <property type="entry name" value="WDR55/POC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019:SF8">
        <property type="entry name" value="POC1 CENTRIOLAR PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44019">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="88"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="130"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="526"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="928" mass="105234" checksum="A72FFA4F370872EC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFP2</accession>
    <name>I7AFP2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">EXS family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_081440</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SYG1 (TC 2.A.94) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265057.1">
        <property type="nucleotide sequence ID" value="XM_009266782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFP2"/>
    <dbReference type="GeneID" id="20521879"/>
    <dbReference type="KEGG" id="ero:EROM_081440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081440"/>
    <dbReference type="HOGENOM" id="CLU_006116_3_1_1"/>
    <dbReference type="OrthoDB" id="9970435at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000822">
        <property type="term" value="F:inositol hexakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016036">
        <property type="term" value="P:cellular response to phosphate starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783:SF103">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 53 MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10783">
        <property type="entry name" value="XENOTROPIC AND POLYTROPIC RETROVIRUS RECEPTOR 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03124">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51380">
        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="474"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="540"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="9">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="8">
        <location>
            <begin position="439"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="633" mass="74780" checksum="3DD7CBB51F75DC53" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFX8</accession>
    <name>I7AFX8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_090070</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265122.1">
        <property type="nucleotide sequence ID" value="XM_009266847.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFX8"/>
    <dbReference type="GeneID" id="20521946"/>
    <dbReference type="KEGG" id="ero:EROM_090070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090070"/>
    <dbReference type="HOGENOM" id="CLU_005180_0_0_1"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 Ig-like" evidence="8">
        <location>
            <begin position="451"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="9">
        <location>
            <begin position="562"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 2 TPR repeats" evidence="9">
        <location>
            <begin position="835"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="764"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="814"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF25577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1131" mass="131052" checksum="02901105736F30E4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG50</accession>
    <name>I7AG50_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Pyruvate kinase</fullName>
            <ecNumber evidence="4 14">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_091040</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 14"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265217.1">
        <property type="nucleotide sequence ID" value="XM_009266942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG50"/>
    <dbReference type="GeneID" id="20564328"/>
    <dbReference type="KEGG" id="ero:EROM_091040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091040"/>
    <dbReference type="HOGENOM" id="CLU_015439_0_1_1"/>
    <dbReference type="OrthoDB" id="108365at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 14 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="15">
        <location>
            <begin position="88"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="504" mass="55520" checksum="D5D589A7A50C7A9D" modified="2012-10-03" version="1">MNIITICFADPSNALAAGTLRDGISSSKDLITTATVFIHPRDGPVDYIHGSWKSMVPICGGPIICLKINLINLESIYPIYPLMKMALTKIVCTIGPSTSSKQKIRDLINAGMSIARLNFSHGTREAHLETIRNIMDSRCNVDRYVAIALDTKGPEVRIRTPEDKDIKVKSGDILHFSSSGRGISIPRIDFKSLSKDDKIFIDDGRIDLRVASVEEDGFRCEVMSNGTIKNDKSMNFPNIDVGIEFLSDEDKDDIIFGLENGIDIIFASFVSSRKDVGKIRKIVGSQVPIVSKIESCLGMKNIREIASSSDGVMIARGDLGVEIGVDKVFSAQKKILLETKKEGKPVICATQMMESMILKNTPNRSETSDVGNAVADGCDCVMLSGESAIGMFPIETVKFMKSICIDAEEYVLGSRPHERYPYVNGVVMCFREALEIEKMYLSSPMIPIVVVSGDKWILRRFSIYRGIVPVDGKGPIDVDRVLQGLKLKGRFLVICKESTKVTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGC7</accession>
    <name>I7AGC7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Myosin heavy chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_092010</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265312.1">
        <property type="nucleotide sequence ID" value="XM_009267037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGC7"/>
    <dbReference type="GeneID" id="20564425"/>
    <dbReference type="KEGG" id="ero:EROM_092010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_092010"/>
    <dbReference type="HOGENOM" id="CLU_000192_7_5_1"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14898">
        <property type="entry name" value="MYSc_Myo37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002710">
        <property type="entry name" value="Dilute_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF706">
        <property type="entry name" value="DILUTE CLASS UNCONVENTIONAL MYOSIN, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01843">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM01132">
        <property type="entry name" value="DIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51126">
        <property type="entry name" value="DILUTE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0505">Motor protein</keyword>
    <keyword evidence="4 7" id="KW-0518">Myosin</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myosin motor" evidence="10">
        <location>
            <begin position="1"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="Dilute" evidence="9">
        <location>
            <begin position="824"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="713"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="90"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1084" mass="125255" checksum="740BB2CE8118FFDF" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGP6</accession>
    <name>I7AGP6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_101400</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265452.1">
        <property type="nucleotide sequence ID" value="XM_009267177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGP6"/>
    <dbReference type="GeneID" id="20564570"/>
    <dbReference type="KEGG" id="ero:EROM_101400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101400"/>
    <dbReference type="HOGENOM" id="CLU_2073128_0_0_1"/>
    <dbReference type="OrthoDB" id="411372at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045124">
        <property type="entry name" value="Su(sable)-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13119:SF12">
        <property type="entry name" value="PROTEIN SUPPRESSOR OF SABLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13119">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25542">
        <property type="entry name" value="zf-CCCH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="13925" checksum="BBC6D3D46D63C505" modified="2012-10-03" version="1">MLPKLNVPTRLNKSYLLEYRTHKATLRPVQKPQEYERPTPNNYRTILCKFFLMNSCKHGDNCTYSHDTSKFPCKAFHLRKSCSRKDCPFSHAPLSDQAMEEIINEKQETPTSLFKSTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGU4</accession>
    <name>I7AGU4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CTP synthase</fullName>
            <ecNumber evidence="9">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_110330</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="9"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265512.1">
        <property type="nucleotide sequence ID" value="XM_009267237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGU4"/>
    <dbReference type="GeneID" id="20564632"/>
    <dbReference type="KEGG" id="ero:EROM_110330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110330"/>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_1"/>
    <dbReference type="OrthoDB" id="1739076at2759"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003792">
        <property type="entry name" value="PRK05380.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="CTP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550:SF0">
        <property type="entry name" value="CTP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTP synthase N-terminal" evidence="11">
        <location>
            <begin position="17"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase" evidence="10">
        <location>
            <begin position="327"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU810713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN84015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="550" mass="61519" checksum="7CE86B0B7F661CBF" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL70</accession>
    <name>I7AL70_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_010550</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HRD1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263896.1">
        <property type="nucleotide sequence ID" value="XM_009265621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL70"/>
    <dbReference type="GeneID" id="20520681"/>
    <dbReference type="KEGG" id="ero:EROM_010550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010550"/>
    <dbReference type="HOGENOM" id="CLU_009169_0_0_1"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
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    </dbReference>
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        <property type="entry name" value="RING ZINC FINGER PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="TPR_SYVN1_N"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="18" id="KW-0436">Ligase</keyword>
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    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="269"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN82399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="335" mass="37893" checksum="096F5DC51395B436" modified="2012-10-03" version="1">MKKIVAYGVTHFILLAIYTTKASHGSLTMYNILTTTTRNSTVHILHATFLIFLMCTLTGRIIDHVFKSLRSEEISNFNESLLYFLTDFLLVVSTFDNDISFKNGLFFAMLLCVKSLSWLLGTRIKRDVYSSLYSLAYGISLFSGLMAFLFTLSCISSIDGQILFLFEYTLLVIASIKNICIMNLILSDDDDKRSLHNFYIDIAYMSITLLVYIVFIGITSFSYRLPLNLFRSALTILDALVAKIKVFLSYLKLCKDLEKCVEGSGDGFCAICRDDMEIGKKLACGHCFHIECLKMWCERQQTCPICKSTLAFDVRKESFVVGSEYISGIPVTIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALA2</accession>
    <name>I7ALA2_ENCRO</name>
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        <submittedName>
            <fullName evidence="12">SCF ubiquitin ligase and anaphase-promoting</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_010900</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="8962942at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
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        <property type="entry name" value="RING BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
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    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="71" mass="8277" checksum="615EAE137E804387" modified="2012-10-03" version="1">MKIETCAICRNHIMDTCVECQNGVISNDECKVSWGICNHAFHTHCITRWLSSKNVCPLDTKKWVYYNPEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM36</accession>
    <name>I7AM36_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_031170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264236.1">
        <property type="nucleotide sequence ID" value="XM_009265961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM36"/>
    <dbReference type="GeneID" id="20521030"/>
    <dbReference type="KEGG" id="ero:EROM_031170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031170"/>
    <dbReference type="HOGENOM" id="CLU_1669897_0_0_1"/>
    <dbReference type="OrthoDB" id="9995306at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="165" mass="18887" checksum="A2EC35E7BF2D2585" modified="2012-10-03" version="1">MGMAEKSLEGVLDLGIFTNKRVCIDEVLPQNADFLVIGLSSILGLSIFTFRETVDHYRKIANIQDKQLVVKSIYDGGYTESDIIDDVWSATNIYGYRTDGRFQVYRSSTCKKSDWYEYDIIVRVLPLSSGVSADVDGTILIFDRERKYAELRYKILRGKIVYSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM75</accession>
    <name>I7AM75_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040200</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264286.1">
        <property type="nucleotide sequence ID" value="XM_009266011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM75"/>
    <dbReference type="GeneID" id="20521084"/>
    <dbReference type="KEGG" id="ero:EROM_040200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040200"/>
    <dbReference type="HOGENOM" id="CLU_677976_0_0_1"/>
    <dbReference type="OrthoDB" id="2192347at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031522">
        <property type="entry name" value="Mad3_Bub1_I_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17014">
        <property type="entry name" value="Mad3_BUB1_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="406" mass="47078" checksum="9F502CC1D59232AA" modified="2012-10-03" version="1">MMTFDGMDEDELENALLNYIDIPYFRNNPSYISLWQRYYYKTNDPSVLFLMKYKKISQYYHWIYVELSRFFMRNGRYGVCKAVLNVGIACNAYEREVLEDELQRIPMVTNPSPEHEINTLLNPKGFTALGKVWNSYQEVLFYNRKLFLLDQEEVSFEEYRAKTYSGKSRLSLKKYGIKLGIGHMGENDPVDGMSNTLSVGKEIVIDGYVYYVKDVMDGNRYRMICISDGNFEELMYKGDVILHEVPKSSIDLIQRLDSDHVPRFTVKELGSRTFLLYDFYVFGTLESCLDISNSMKSGIVLYFVSQLIEIFGGLEERGCRFTAFSLESLCVSEDCRLKIVDFDLSEDGSKMNYGEVIKGVLLQYSSFPFDEQGVVQGIRGILQKTDLKGLVTKLRIHLYERICKCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMD5</accession>
    <name>I7AMD5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264361.1">
        <property type="nucleotide sequence ID" value="XM_009266086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMD5"/>
    <dbReference type="GeneID" id="20521161"/>
    <dbReference type="KEGG" id="ero:EROM_040970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040970"/>
    <dbReference type="HOGENOM" id="CLU_138075_0_0_1"/>
    <dbReference type="OrthoDB" id="2190490at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="137" mass="16098" checksum="1D202F30554B3063" modified="2012-10-03" version="1">MALVIDTFEVPSLLISRKLFRSLKVMDIEEVSLETIEDVLTVEGIYSIHKECFSYDDIRWMKYYFNEVVEVFANVQYINKSKKVVKYYSIIRDLDGTAIDLVEIKENENEEIHDKTAFLPYIKAQGEEIVIFPDDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMF8</accession>
    <name>I7AMF8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome production factor 2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Ribosome biogenesis protein RPF2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_041230</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
            <location evidence="1 4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RPF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264386.1">
        <property type="nucleotide sequence ID" value="XM_009266111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMF8"/>
    <dbReference type="GeneID" id="20521186"/>
    <dbReference type="KEGG" id="ero:EROM_041230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041230"/>
    <dbReference type="HOGENOM" id="CLU_100680_0_0_1"/>
    <dbReference type="OrthoDB" id="407658at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039770">
        <property type="entry name" value="Rpf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728">
        <property type="entry name" value="BRIX DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12728:SF0">
        <property type="entry name" value="RIBOSOME PRODUCTION FACTOR 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Brix" evidence="5">
        <location>
            <begin position="40"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="241" mass="28168" checksum="BA088E46FFA03559" modified="2012-10-03" version="1">MRVKHPSERKTLLSVICSDKGEKIQREIGVIRGDTVHIHEKVNPFDCAEQIEKLMKKKKSGMFISITKDSLLVIGRTFNDEIIDMVEFKINRYLSVTDFECVGPELHMKYFVVLQNINNKRLENLIVDFFNKKSSKVCLEGIRYSWVFTKTEDGYVLKYVRVLKDLNVEDCGPLLEMELVRSYHCSDELYKKALDEPKKPKKNISKNLFNDKIGTLHIDKQDLRDIRLKKSKGYKRTSSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMG7</accession>
    <name>I7AMG7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264396.1">
        <property type="nucleotide sequence ID" value="XM_009266121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMG7"/>
    <dbReference type="GeneID" id="20521196"/>
    <dbReference type="KEGG" id="ero:EROM_041330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041330"/>
    <dbReference type="HOGENOM" id="CLU_1042165_0_0_1"/>
    <dbReference type="OrthoDB" id="2190670at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="2">
        <location>
            <begin position="115"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="267" mass="30939" checksum="58061EC0A0E8EF66" modified="2012-10-03" version="1">MEQAVANLGLILESRAENKSKMVMEALEGLVKSILGSSYEEIANYELDEMLTESFDKTVCEDHKRFVEMLPDLVSCMRDPRNIFPAIERYFNPECDFSIDVAKVVFVMKRDFGFEFDGFHSTLFDCITSRNIEEDIEKKLLFILMTLEDGSTPLAVAKAFIKKLCNISLQVKSSHCHKILWTILWIMRFHPMTYVMARKESFRKDLEWAVSIEMDSFQPYLFELDILGESLEGIKKIVNLIKREAVHAKSRPKLLSLTDISFPRLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AML1</accession>
    <name>I7AML1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RPS1</name>
        <name evidence="6" type="ordered locus">EROM_050210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (25S, 5.8S and 5S).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264451.1">
        <property type="nucleotide sequence ID" value="XM_009266176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AML1"/>
    <dbReference type="GeneID" id="20521253"/>
    <dbReference type="KEGG" id="ero:EROM_050210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050210"/>
    <dbReference type="HOGENOM" id="CLU_062507_0_2_1"/>
    <dbReference type="OrthoDB" id="9834376at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03122">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_eS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027500">
        <property type="entry name" value="Ribosomal_eS1_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11830">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="initiator methionine" description="Removed" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylalanine; partial" evidence="4">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="239" mass="27040" checksum="F7FB273BB8EE1D89" modified="2012-10-03" version="1">MAIQPPGSYPQGNRKGKAKKKTGQDSFARKEWYNLRSPSIFPSNINGKTLVSKATGKNAHMRAIGRRYDVNHADLTKNNDVAHRKFIFKIGDVKGADCIGFFDGMELTSDKHKAMIKKWHSLIEAQKDIVAKDQSVFRVFVMAVTKRPHGYTKKTCYVKHSDEKRIRKIMFDVIEEELGGRSVDDIMSKLSNETVGKRIEELGSEIFPLQNCCVRKVKTIKSYQTATITQQDEVKAAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AML8</accession>
    <name>I7AML8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BCAP29/BCAP31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264461.1">
        <property type="nucleotide sequence ID" value="XM_009266186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AML8"/>
    <dbReference type="GeneID" id="20521263"/>
    <dbReference type="KEGG" id="ero:EROM_050310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050310"/>
    <dbReference type="HOGENOM" id="CLU_120104_0_0_1"/>
    <dbReference type="OrthoDB" id="435607at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070973">
        <property type="term" value="P:protein localization to endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008417">
        <property type="entry name" value="BAP29/BAP31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040463">
        <property type="entry name" value="BAP29/BAP31_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701">
        <property type="entry name" value="BCR-ASSOCIATED PROTEIN, BAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12701:SF20">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05529">
        <property type="entry name" value="Bap31"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="BAP29/BAP31 transmembrane" evidence="7">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU367026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="199" mass="23706" checksum="90F4041925003C6C" modified="2012-10-03" version="1">MGITTQLVQSIVFGEMCMFTFMLLPISKNLKKKVIKLFQTSKFYRGFLHIIYVLFAMILIMFVDSAYKIYTGEDQINPFVLYQAERNMYLTGFTLFLGVIFRMFIRTMCLLFREEESAQLLRKQSMNQKKYVEEMLEDNTKKAKRIEELEVEIIDLKKKILSGDILIKQLKNNQNEYFSLLDKYNGLREQMQMESRKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMY5</accession>
    <name>I7AMY5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">leucine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_060190</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264611.1">
        <property type="nucleotide sequence ID" value="XM_009266336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMY5"/>
    <dbReference type="GeneID" id="20521416"/>
    <dbReference type="KEGG" id="ero:EROM_060190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060190"/>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_1"/>
    <dbReference type="OrthoDB" id="10249672at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794:SF1">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="11">
        <location>
            <begin position="24"/>
            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="873" mass="101913" checksum="9BF349AF74A1C7D8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN56</accession>
    <name>I7AN56_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_060650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264656.1">
        <property type="nucleotide sequence ID" value="XM_009266381.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN56"/>
    <dbReference type="GeneID" id="20521462"/>
    <dbReference type="KEGG" id="ero:EROM_060650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060650"/>
    <dbReference type="HOGENOM" id="CLU_030733_4_0_1"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0697">Rotamase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="241" mass="28209" checksum="E069ED6F65CBA999" modified="2012-10-03" version="1">MKDGIDFTETEAYARIYNFILMVDDSIKSSKQNQSRQHRDLLASIAEIIKETEKDQTPQRYANAAAKIVFKRICDEHDDEYLRNSFGNKIRLDYGTGHELNFLCYLYNQYCEGAITIDCVFTTLVEYFEVVRLFVTKFNLEPAGSHGIWGLDDYQFLPFLFGSSELCNTRLRFDELDDTKCYFVAVKRKLGGSSQILKSIMDKDWATINRGMIRMYDDYVLKKDVVTQHFIYGRYLRKEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN85</accession>
    <name>I7AN85_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_061070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264696.1">
        <property type="nucleotide sequence ID" value="XM_009266421.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN85"/>
    <dbReference type="GeneID" id="20521503"/>
    <dbReference type="KEGG" id="ero:EROM_061070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061070"/>
    <dbReference type="HOGENOM" id="CLU_140672_1_1_1"/>
    <dbReference type="OrthoDB" id="9616667at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="94" mass="10809" checksum="909FF1B47E0CFC7C" modified="2012-10-03" version="1">MKNAYKKVRVRYPVKRPDVKRKPKKLSEEFRKARMLAASVAEEIGGLSPLEKKVVSLIEAKNNNKAQKILKVRLGSHKRAGIRIEKLTKMILEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANB6</accession>
    <name>I7ANB6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264741.1">
        <property type="nucleotide sequence ID" value="XM_009266466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANB6"/>
    <dbReference type="GeneID" id="20521550"/>
    <dbReference type="KEGG" id="ero:EROM_061530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061530"/>
    <dbReference type="HOGENOM" id="CLU_559007_0_0_1"/>
    <dbReference type="OrthoDB" id="2192420at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="360"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="495" mass="56328" checksum="4AA0B21823C70684" modified="2012-10-03" version="1">MLLKLLFIPVIICNRLLRLKHKDGQYVTNSKGVLKLELPMLPSLSDQRVVVHNRVLYFFKDGIYYTLAMVHGELEIKLIVYNAMSEKGIGFSSNPTFRPRKRKIDPFGYLSHAGHRDNQSDGEKEKDSLLSNKRKPGLEKPPFRNERLSPQKRIERVSKSRPRDHEEYRGRQETLDGKEAHQAKKDKPSEVDTAERNNKVLKDGFDETEALDGSDKVSGYNAPPLRLEVETSSTLRSIRFPKKTDSPRRFYVVESDSKMDEREFGYIIKNVVVSEVDNQGHFTLIGGNDACVTYYKDSFVFMPCSRAPEQIFKLESSEEIVKKGKFLGLGKDKVVENQDAVILDSTDSEDERKEELYKNPQALPYSLKSTGHDSSSFGTPNIKVGSKDRARYLDGSLDKENPHVDYLKRNGRDDLSRGALQFENSHKSLNIDTKPTKTATGVTPISNPSTQARSSRKGSDSNSKEANRHQKLNLDDDFIDKFRELLTNSDLNDFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANC6</accession>
    <name>I7ANC6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264756.1">
        <property type="nucleotide sequence ID" value="XM_009266481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANC6"/>
    <dbReference type="GeneID" id="20521566"/>
    <dbReference type="KEGG" id="ero:EROM_070080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070080"/>
    <dbReference type="HOGENOM" id="CLU_946747_0_0_1"/>
    <dbReference type="OrthoDB" id="189968at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="299" mass="33090" checksum="60CA93D141668933" modified="2012-10-03" version="1">MIPAIAMILTKVVERKLSGAPTSLYLTESKIYVSLKKKYVIETGYNALKLRRIPVKENIRVMVGCEENIFLFSDKGGFYIFLHEGNIRNGSTFGKKIINTGLYNQSSGHIVIGTNKGKILVLTQNFEVWKTFYETPSGVIDLSISGLGKIACIYEIDPTVRIFDLKHSEVKSIKIPSGFPQAVAFVNGTHLAIGNDAGEIHLLDTTVMKIQHSSTIPQAITTLTWRDGLLFIGGEDSLYLSSIAENGIDIMNSYKCNGFVNAVCLNDNHIVVGVGKEPRLGRWKVKKDGEHKLIILRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANZ5</accession>
    <name>I7ANZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265016.1">
        <property type="nucleotide sequence ID" value="XM_009266741.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANZ5"/>
    <dbReference type="GeneID" id="20521838"/>
    <dbReference type="KEGG" id="ero:EROM_081040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081040"/>
    <dbReference type="HOGENOM" id="CLU_2145834_0_0_1"/>
    <dbReference type="OrthoDB" id="2190037at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="112" mass="12983" checksum="E44D47D831E53436" modified="2012-10-03" version="1">MEKNDYLKLEKAIRDGECLESLSVLRMVLDRVVNDISASCPRDDLELLSKSIQHQRLVYEKAVSLYEGMQAESSERDGLIKCLEDFNQTLEEYYAFSGKLKNQNTEEEKDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7APB4</accession>
    <name>I7APB4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA repair and recombination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_090420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265156.1">
        <property type="nucleotide sequence ID" value="XM_009266881.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APB4"/>
    <dbReference type="GeneID" id="20521981"/>
    <dbReference type="KEGG" id="ero:EROM_090420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090420"/>
    <dbReference type="HOGENOM" id="CLU_707938_0_0_1"/>
    <dbReference type="OrthoDB" id="413460at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008094">
        <property type="term" value="F:ATP-dependent activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050496">
        <property type="entry name" value="SNF2_RAD54_helicase_repair"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629:SF7">
        <property type="entry name" value="DNA EXCISION REPAIR PROTEIN ERCC-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45629">
        <property type="entry name" value="SNF2/RAD54 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="155"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="390" mass="45336" checksum="F7D57EF4300BFFD2" modified="2012-10-03" version="1">MDCRAEDYEKSFVEKYLERTAAEKIDELLRKIEMHKRKGDVEKVKELEKEIEEVKKGLVDDKSIVLRVEDRKEDAGSNEAIDFEWSGSDDDENIEVFNSRCEKAIGKYCRHMGIGRHEFESHLKKESHTRQGFLVANFLWDTLFEYQQVGVEWMLRLYKDGKGGVLADDMGLGKTIQMIAFLAILFQSKSISKVLILCPATIVSQWTLEWKRFYPFVRVFFGFSGRNCEGVYLMSYERFKVRAKDLLWDVLVLDEGHKIKNRNAQITLSVKKVRAKSKFVLSGTPIQNNLGELWSMFDFVNPGLLGSHTSFHEEFEEVIRRGGYKSATNLQVEKAYKHSLMLRSLIEPYILRRTKSQISHKLPSKEDKIVFCTLTPVQIELYNRVLSLSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7APH8</accession>
    <name>I7APH8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265241.1">
        <property type="nucleotide sequence ID" value="XM_009266966.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APH8"/>
    <dbReference type="GeneID" id="20564352"/>
    <dbReference type="KEGG" id="ero:EROM_091280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091280"/>
    <dbReference type="HOGENOM" id="CLU_410540_0_0_1"/>
    <dbReference type="OrthoDB" id="2191694at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="597" mass="69505" checksum="BADC1DEC8FF59AC7" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7API9</accession>
    <name>I7API9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265261.1">
        <property type="nucleotide sequence ID" value="XM_009266986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7API9"/>
    <dbReference type="GeneID" id="20564372"/>
    <dbReference type="KEGG" id="ero:EROM_091480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091480"/>
    <dbReference type="HOGENOM" id="CLU_098502_0_0_1"/>
    <dbReference type="OrthoDB" id="2192887at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="223" mass="25471" checksum="488CAD42A64B17A5" modified="2012-10-03" version="1">MTTTAFKNVRSLSQLVSVLLIVINIPLPFFLIPHLMFVRMEAEHLRMGIRGITTIFRTTSMTNITLGLFASCGINSKTRLYMRLYFITGIFLFLMLITMLLYIRISYQSDVSRQLGRLYNNASIRNIILYFMECTNQMTCTRIIMKNISMIKYYYVTIAIPSLCLNLLNLILIRIAVSINIETPPPKLPNFVEKTRVGMNTSSLRNKRVVINGPGQEGQGTVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7APJ4</accession>
    <name>I7APJ4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_091580</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265271.1">
        <property type="nucleotide sequence ID" value="XM_009266996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APJ4"/>
    <dbReference type="GeneID" id="20564382"/>
    <dbReference type="KEGG" id="ero:EROM_091580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091580"/>
    <dbReference type="HOGENOM" id="CLU_047877_2_2_1"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="206" mass="24071" checksum="58A463579EE1A30C" modified="2012-10-03" version="1">MEIEIGKEAIRRSTEYANKGLGAVSLKSFRTYFDIDNTFVLKKLVLILFPFHNKEWVSEDEGMARPELYVPIMSFISYVLIRALRLGFEGMFSPEKLGIVFTRLFFLEAMCVLFTRVSGYFFDVTLSTLDVAAYSGYKYVIAVLLQINRMQYVRIIGGMYLYVSFFIFLSRSLKRRVMDRGAERMRRAYYLFGIVIVQEFIVFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7APJ7</accession>
    <name>I7APJ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_091630</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265276.1">
        <property type="nucleotide sequence ID" value="XM_009267001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APJ7"/>
    <dbReference type="GeneID" id="20564387"/>
    <dbReference type="KEGG" id="ero:EROM_091630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091630"/>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17940">
        <property type="entry name" value="DEADc_DDX6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47960">
        <property type="entry name" value="DEAD-BOX ATP-DEPENDENT RNA HELICASE 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 14" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="13">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="141"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="321"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="8">
        <location>
            <begin position="110"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="491" mass="55525" checksum="976FB875FFD185DA" modified="2012-10-03" version="1">MPMKSMVEDVKKDRKRNGRNSHERTWKKGALQERCEEKKSSSLNDIEEFGNEVRSSKRKGDGNHLCIKDNEKEEKRTESITSGETSSSNRSLEESIKRLLTKDVRPTDNIRWEMFGLRPLLLKRIYTIGYEFPSPVQAASIPRALKGRNLLVRSKNGTGKTASYVIPMLNAINPDELSIQGIILVPIRELALQISRNVKRLSEGMGVASVPVVGGTNMQDDIIRVSNGVHVMVGTPGRIVDLVEKKIGVISKKVILVFDEADKLLDVTFGETVTRLLELLPRERQILLYSATFPYFVTGFIKRYMRDPLCINLMKELAPIGIKQFYTQVKPSEKLICLRSLLLKLKINQCVIFCNNIRTVELLAMRITGMGMPSYFIHSKMTQEDRNIVFHNFLKGKCKILVATDLITRGVDVPNTNYVINFDIPKSPESYLHRIGRAGRFGTRGVAISLVTIEEREMLADIEAKLGKEISPLSDGGLNRLYESNIDKDQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7APN2</accession>
    <name>I7APN2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">T-complex protein 1 subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_100110</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265326.1">
        <property type="nucleotide sequence ID" value="XM_009267051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APN2"/>
    <dbReference type="GeneID" id="20564441"/>
    <dbReference type="KEGG" id="ero:EROM_100110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100110"/>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000005">
        <property type="entry name" value="T-complex protein 1 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012719">
        <property type="entry name" value="Chap_CCT_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02344">
        <property type="entry name" value="chap_CCT_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="519" mass="57567" checksum="4EB2D7D432C902CE" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ20</accession>
    <name>I7AQ20_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNase P subunit Rpr2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_101590</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265471.1">
        <property type="nucleotide sequence ID" value="XM_009267196.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ20"/>
    <dbReference type="GeneID" id="20564589"/>
    <dbReference type="KEGG" id="ero:EROM_101590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101590"/>
    <dbReference type="HOGENOM" id="CLU_079140_4_2_1"/>
    <dbReference type="OrthoDB" id="128536at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="96" mass="11081" checksum="9745228775F2A6FA" modified="2012-10-03" version="1">MKKDFAKHLMYLYCTAFRTVGIPSVHRRKMKKIKMISNKYQIRLSREIKRTVCPGCYTILIPGFNCVSRVTREENGLCLKTSCSCGNEKVFVAQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ25</accession>
    <name>I7AQ25_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263909.1">
        <property type="nucleotide sequence ID" value="XM_009265634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ25"/>
    <dbReference type="GeneID" id="20520695"/>
    <dbReference type="KEGG" id="ero:EROM_010680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010680"/>
    <dbReference type="HOGENOM" id="CLU_1454395_0_0_1"/>
    <dbReference type="OrthoDB" id="2190134at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21171" checksum="E84FE7411AF6C79A" modified="2012-10-03" version="1">MSIEEIKGSTEENGGGGEDKKDREQCMDNCKDNEEVSNIFKPGDLSDLRSKKTIFEDLGGEKKQPKESEFLKTPVSKSSTPVQNEIPGVVFKAGANLHKFVGEWENIGPGFVYVTRNKEKRCFFIRDGVMMCAFDFLVTYDTRPTKKKLGVCIGVREMIENKCVEQPYCVVFKNEKDASEFVQTMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ35</accession>
    <name>I7AQ35_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">WD40 repeat-containing anti telomeric silencing-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_010780</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263919.1">
        <property type="nucleotide sequence ID" value="XM_009265644.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ35"/>
    <dbReference type="GeneID" id="20520705"/>
    <dbReference type="KEGG" id="ero:EROM_010780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010780"/>
    <dbReference type="HOGENOM" id="CLU_663974_0_0_1"/>
    <dbReference type="OrthoDB" id="1367865at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045183">
        <property type="entry name" value="Ebi-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846:SF2">
        <property type="entry name" value="F-BOX-LIKE_WD REPEAT-CONTAINING PROTEIN EBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22846">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="262"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="301"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="414" mass="45946" checksum="E84FBCCE6C2DC559" modified="2012-10-03" version="1">MIEMSRDELNQLVYKYLEDEGYIYTLFTFKNEAAPSDKPLGHSLVSLVGKGLQYIYGTEHLNGNEVVECDAKFCLSEEHVCKFKKKEDASADALYGLKKEGTMVVDVEEVRLGNVGSELLSCWNKEWLGIYTTSGEVLGLGSEGLLWKREMKKVTALSWSDDNLVVGNEGGEVITVNISSGRTRSHTCHSKAVTKVKQRGMGILSSGRDGRIVMMNNGVTEMSVSECGVEDIAWVGENEVGCSLSDFSVSFVNSNELKVSKVGTHEDKISNIEHNEEILSTSSHDGTLGLWDIVSMNGDRMNVHDGRVNCHKWMNGRIVTCGSDRFVKVWDMEKAMPIHELNHEDEVISVDCSSRLVASGSLDGRVILSDYRCNEAYRYKADGSISKVLFSESGSYLCICISGHPPKLINLRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ43</accession>
    <name>I7AQ43_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NOL1/NOL2/sun tRNA/rRNA cytosine-C5-methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_010880</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263929.1">
        <property type="nucleotide sequence ID" value="XM_009265654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ43"/>
    <dbReference type="GeneID" id="20520715"/>
    <dbReference type="KEGG" id="ero:EROM_010880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010880"/>
    <dbReference type="HOGENOM" id="CLU_005316_7_0_1"/>
    <dbReference type="OrthoDB" id="427002at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009383">
        <property type="term" value="F:rRNA (cytosine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031341">
        <property type="entry name" value="Methyltr_RsmF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF30">
        <property type="entry name" value="28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17125">
        <property type="entry name" value="Methyltr_RsmF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="8">
        <location>
            <begin position="24"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="115"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="306" mass="34180" checksum="882F51822BDD3003" modified="2012-10-03" version="1">MDTYNEYLLEKICELFPRKEAEVFIEESEKQRPTTIRINTLLKHRKDVSKLLRGRGVDLDDLSWTDSGCVVFKSSVPIGATPEYLAGYYCLQGAASMLPVLNMELKEGLTVVDLCAAPGGKTTHIAALMENTGIIYANDVNEDRMAGLKSNIQRMGVRNCIVMNMDGRKVNVGKVDRVLLDAPCSGTGVISKDPSVKTTRTRSEIDRMATLQKELILHGFEMLRPGGILMYSTCSVLVKENEEVVNYLLTKCPSAKMAQCEVDIGKDGFMSFKGRNYNGSLKMARRIYPHVHNMDGFFYAKIVKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ90</accession>
    <name>I7AQ90_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pre-rRNA-processing protein PNO1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_110620</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PNO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265541.1">
        <property type="nucleotide sequence ID" value="XM_009267266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ90"/>
    <dbReference type="GeneID" id="20564661"/>
    <dbReference type="KEGG" id="ero:EROM_110620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110620"/>
    <dbReference type="HOGENOM" id="CLU_064992_1_0_1"/>
    <dbReference type="OrthoDB" id="1932641at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22391">
        <property type="entry name" value="KH-I_PNO1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055212">
        <property type="entry name" value="KH-I_PNO1_first"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="8">
        <location>
            <begin position="88"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="22922" checksum="B639EC23C2BD1A1D" modified="2012-10-03" version="1">MTEIQTRSIPIHPRKMRGMKEEWMRIYTPIVEICKIQIRMNIKGRCVEMRTCEHTEDPSYLERSAQYIEAVHIGFPIEDAIAILKFSDVFLDRFEISEVKTLRGLHIERAIGRIIGREGKTKNAIEEFSRSKIIVQDQMIHLLGTIENTRIARDAVCRLIMGSQPGSIFNRLRIINSRLKEKYGGIQTIYNEFEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ94</accession>
    <name>I7AQ94_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Minichromosome maintenance 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_110670</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265546.1">
        <property type="nucleotide sequence ID" value="XM_009267271.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ94"/>
    <dbReference type="GeneID" id="20564666"/>
    <dbReference type="KEGG" id="ero:EROM_110670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110670"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="OrthoDB" id="7462577at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd22247">
        <property type="entry name" value="MCM8_WHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056875">
        <property type="entry name" value="MCM8/REC_WHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF47">
        <property type="entry name" value="DNA HELICASE MCM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25051">
        <property type="entry name" value="WHD_MCM8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="9">
        <location>
            <begin position="286"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="682" mass="76243" checksum="9522FA817AFB1C59" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ99</accession>
    <name>I7AQ99_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase</fullName>
            <ecNumber evidence="10">2.7.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_110720</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Functions in the biosynthesis of the anionic phospholipids phosphatidylglycerol and cardiolipin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>a CDP-1,2-diacyl-sn-glycerol + sn-glycerol 3-phosphate = a 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12593"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CDP-alcohol phosphatidyltransferase class-II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="10"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265551.1">
        <property type="nucleotide sequence ID" value="XM_009267276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ99"/>
    <dbReference type="GeneID" id="20564671"/>
    <dbReference type="KEGG" id="ero:EROM_110720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110720"/>
    <dbReference type="HOGENOM" id="CLU_746023_0_0_1"/>
    <dbReference type="OrthoDB" id="10250191at2759"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00503"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09137">
        <property type="entry name" value="PLDc_PGS1_euk_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016270">
        <property type="entry name" value="PGS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586:SF1">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12586">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000850">
        <property type="entry name" value="Phospholipase_D_PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="11">
        <location>
            <begin position="117"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN84054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="371" mass="43392" checksum="8E411E3D292E773F" modified="2012-10-03" version="1">MNHLNGKSKKLDSVHVSDFRLLGDCTEFYQRIKFLLQTSESVYMASLFFGQDGEMEDILDILEEKKRRGLITVIFIDKNRGAAGRRLEFIQERGLESTFHLIDLSTSCLFPAKLNELFRVFHTKALVFDDITILSGANMDASYMVNRIDRYLEIQSKELTDMIKTKVFGVELSPDVESTPIQNLPIYVRMFKQQEEQSIVRSFLRSFGEIHISTGYLNFPDNYLKMFKGRRISLYASCPDRNRFNSFGIMDRFVGPIYSYSFHRTLECIPTLSIYELNKKGYSFHLKGFWGFKDKTAATIIGSSNFNRRSTERDDETNYLIVTSDPGHIQKLREEVEYLRANSNPRSLKQLRKRTYRLIVIILFLIFNRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQA6</accession>
    <name>I7AQA6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">U1-like Zn-finger-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_020220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ZNF593/BUD20 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263994.1">
        <property type="nucleotide sequence ID" value="XM_009265719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQA6"/>
    <dbReference type="GeneID" id="20520780"/>
    <dbReference type="KEGG" id="ero:EROM_020220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020220"/>
    <dbReference type="HOGENOM" id="CLU_117291_4_1_1"/>
    <dbReference type="OrthoDB" id="24683at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051879">
        <property type="entry name" value="C2H2-ZF_Maturation_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46095:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="104" mass="12176" checksum="3E0F4E526ED47FE3" modified="2012-10-03" version="1">MPKSDTKQKKGRKNRNRLRIKRTKVFGPKGIDQVKSQVESRKRVEYDPELPGGGQFYCYECDRHFISEDVLIGHRKSSLHRKRIKEVREPAHSQKDAEWAVGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQB4</accession>
    <name>I7AQB4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_110870</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="2"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265566.1">
        <property type="nucleotide sequence ID" value="XM_009267291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQB4"/>
    <dbReference type="GeneID" id="20564686"/>
    <dbReference type="KEGG" id="ero:EROM_110870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110870"/>
    <dbReference type="HOGENOM" id="CLU_2386140_0_0_1"/>
    <dbReference type="OrthoDB" id="7759664at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045191">
        <property type="entry name" value="MBR1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937:SF65">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE ARK2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22937">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF165"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="9">
        <location>
            <begin position="47"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN84069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="90" mass="10327" checksum="98715690DC2B95C0" modified="2012-10-03" version="1">MLDCDSGEEAEVIAKKLLNIGEYRDAEFLSVVLDEMAEADEVEKEKCCICLEYIKESKALLGCNHSFHLNCIYLWFNMKRTCPSCRKAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQH6</accession>
    <name>I7AQH6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264064.1">
        <property type="nucleotide sequence ID" value="XM_009265789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQH6"/>
    <dbReference type="GeneID" id="20520853"/>
    <dbReference type="KEGG" id="ero:EROM_020920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020920"/>
    <dbReference type="HOGENOM" id="CLU_073203_1_1_1"/>
    <dbReference type="OrthoDB" id="2415936at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08513">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="1">
        <location>
            <begin position="62"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="226" mass="26289" checksum="EFECE02B12ED79FE" modified="2012-10-03" version="1">MSFDKMKSKSYEKKMEEWLTMVSMKVVDLNRLVLDYLVHEGLGDVATEFAKDVGIPFTVSSFLNHRTRIRNAIEEGNIDMAISRINDLNSEIIDSNIELYYFIMEQKACEQAQAIRNDSESIGEQKVFVLLEEVLEFVRSELSSIVEENPSLGLHFEDFLEFVVFNSRKEAVVERRRRLAGYVNRCILEKYEVTENELKRVLSGIVEGEKLLTEKYKFPTFNESFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQI7</accession>
    <name>I7AQI7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Splicing branch point binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_021070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264079.1">
        <property type="nucleotide sequence ID" value="XM_009265804.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQI7"/>
    <dbReference type="GeneID" id="20520869"/>
    <dbReference type="KEGG" id="ero:EROM_021070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021070"/>
    <dbReference type="HOGENOM" id="CLU_1348917_0_0_1"/>
    <dbReference type="OrthoDB" id="6777263at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045071">
        <property type="entry name" value="BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055256">
        <property type="entry name" value="KH_1_KHDC4/BBP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11208">
        <property type="entry name" value="RNA-BINDING PROTEIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22675">
        <property type="entry name" value="KH-I_KHDC4-BBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="74"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="209" mass="24324" checksum="31EB82C041E73CCA" modified="2012-10-03" version="1">MESFRYPEKVALPEGVKDKSGLKDLINTIRTEEIDRRLQAAVASNDQGLVKWLKARKQHILGHQVQGNVTISGSMYTNKIYIPIQEFPESNFVGLIIGPRGSTQKQLERITRTRIYIRGSYKDKYAEPLHCYITADTQESLLNATAVIENLIEDSILFGDTRLKTSQLRAMREHKMQKGFSDWEKYYYWWYFHNKIQRKAECSNSLSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQY8</accession>
    <name>I7AQY8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome regulatory subunit 7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_031200</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264239.1">
        <property type="nucleotide sequence ID" value="XM_009265964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQY8"/>
    <dbReference type="GeneID" id="20521034"/>
    <dbReference type="KEGG" id="ero:EROM_031200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031200"/>
    <dbReference type="HOGENOM" id="CLU_000688_6_1_1"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0647">Proteasome</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="192"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="415" mass="46761" checksum="BE437431337FBAD1" modified="2012-10-03" version="1">MSGQKQENIIEEKDVGFILSYGRNYYAEEIMEREAEIKRIFEQISLKLGTKEVETGLAPPMCWDLLGDRNRIQSEPNLQVARVCKVLDGYQEPRYMIGIKMIAKFIVGKGKRVDGSLIQEGMRVGVDRNKYQIILPLPRKIDASVTLMQVEERPDVTYNDIGGCKEEIEKIREVVEAPLLNPERFVALGIDPPKGVLLYGPPGTGKTLLARAVANRTNACFIRVIGSELVQKYVGEGARMVREIFAMAKGKKACIIFFDEVDAFGGTRFDDEDDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALLRPGRLDRKVEFGLPDLEGRTSILRIHAKTMSVDKNIRFDLIARLCNNATGAELRSVCTEAGMFAIRERRKIATEADFLKAVDKVIKGYAKFSSTPRYLTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQZ1</accession>
    <name>I7AQZ1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pelota-like RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_031250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264244.1">
        <property type="nucleotide sequence ID" value="XM_009265969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQZ1"/>
    <dbReference type="GeneID" id="20521039"/>
    <dbReference type="KEGG" id="ero:EROM_031250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031250"/>
    <dbReference type="HOGENOM" id="CLU_023334_1_0_1"/>
    <dbReference type="OrthoDB" id="10249111at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="347" mass="39635" checksum="3BD7C7B598B97A74" modified="2012-10-03" version="1">MKLEKNRIDQRCMSGSLRMTAEHQDDIYILSSVIDVGDMVTSTTTRKIQIDGKTQYRMSLVLKIKIESINVDLESSTIYLKGKTVLINEYVRLGSYHTIDVTLGQSFELEKEEWQEMSIKLLKEAAKPQPEVVFIIFYEKECVVSMVTRSKISVILKQETKSKKFGNVIKVLEKYIGKVSLFVIASIFEIRNEFYKAAMGSRELKKACGSFCVIKVPPECRGCSNSKVINMVLTDKELSKTFQGVQYIDDLKEIDNFFVKFAKGSDLICIGMESIREAMDYGALDKLMITDEKIKPNDVEERREMEVFCKEIQAMNCKISIIPVAHFSGERLKEMGGICGLLKFNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR55</accession>
    <name>I7AR55_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_040640</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264329.1">
        <property type="nucleotide sequence ID" value="XM_009266054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR55"/>
    <dbReference type="GeneID" id="20521128"/>
    <dbReference type="KEGG" id="ero:EROM_040640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040640"/>
    <dbReference type="HOGENOM" id="CLU_014464_2_0_1"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000021">
        <property type="entry name" value="M-phase inducer phosphatase cdc-25.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="10">
        <location>
            <begin position="144"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="265" mass="30680" checksum="F3E93CE4B8F6F237" modified="2012-10-03" version="1">MTNSSAFTSSDNDQYPTAVDQMHIKPFYEMLWSSSDNIELEAPANDPFVDIDAFDSIEMGRPGLFVKKGKFREKGMWTSLHATQNQNRVSSAPIVRRHLIDFWKDFSNGEVFSLPTLGPGKSDSIQRISVDVTKQVIEGMYNIDYLVIDCRFPYEYQGGHIMNAVNISSTRELGLLFRKPKALIFHCEFSSIRAPRLAQHLRNMDRMKNPYPLLTIPEVYVMEGGYRKFYSKYPGLCDPKGYVAMDSLDYRDLYKRNKFRSNKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR97</accession>
    <name>I7AR97_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dTMP kinase</fullName>
            <ecNumber evidence="3">2.7.4.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_041210</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="3"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264384.1">
        <property type="nucleotide sequence ID" value="XM_009266109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR97"/>
    <dbReference type="GeneID" id="20521184"/>
    <dbReference type="KEGG" id="ero:EROM_041210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041210"/>
    <dbReference type="HOGENOM" id="CLU_049131_3_2_1"/>
    <dbReference type="OrthoDB" id="425602at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF1">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="9">
        <location>
            <begin position="9"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="191" mass="21901" checksum="D1DAA8162B10BAD4" modified="2012-10-03" version="1">MSKGKFVVFEGLDRSGKTTIVNTLYESLLPTVSVNKIRFPNRNGCVGKVIDEYLQKKISLKNETIHLLFSADRYEHKAMIEELRKDSIVLCDRYSMSGVAYSVAKGLSLDWCAMSESLLPKPDLTVFIDVPMDEICRRKEFGKEIHDSITFLKKVFLAYQILLKEEKNVFIVDGTLPIDEIIEIVKKKIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARD1</accession>
    <name>I7ARD1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264424.1">
        <property type="nucleotide sequence ID" value="XM_009266149.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARD1"/>
    <dbReference type="GeneID" id="20521226"/>
    <dbReference type="KEGG" id="ero:EROM_041630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041630"/>
    <dbReference type="HOGENOM" id="CLU_983617_0_0_1"/>
    <dbReference type="OrthoDB" id="2747330at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="283" mass="33002" checksum="30DBF9869710276A" modified="2012-10-03" version="1">MFYLQFNIIIIPKMGYMIRTALFSSDILHIEFIECDLFMCVGSTLYILNMKGLIREDVSLPSNEVREIFKFKDVVFLQIEKHGYYYFKIDSPMLTKARWGIEKMHVNSNAIFSHGCVIFFLDSSTLQPNHIVTMPSKIIDFAEVGSDIYVLVETHLCMIQNWKLNRENVLIKSIEFLSHLKFVSVVSNGLDLLVLENSYEMVVYGKEVLRPIKKKSNYQGFKVFGTLFILLLSDSVELYCSVTHKMLTKLCFTPKAIAYDSYRRRLWLHSDFLYEIDVSKITI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARG7</accession>
    <name>I7ARG7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Histone-binding protein N1/N2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_050340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NASP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264464.1">
        <property type="nucleotide sequence ID" value="XM_009266189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARG7"/>
    <dbReference type="GeneID" id="20521266"/>
    <dbReference type="KEGG" id="ero:EROM_050340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050340"/>
    <dbReference type="HOGENOM" id="CLU_069888_0_0_1"/>
    <dbReference type="OrthoDB" id="5587616at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="154"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="247" mass="28952" checksum="4CF08933A0EF7FF1" modified="2012-10-03" version="1">MEDLAEAFPMLSEARASIAQGEFEHGIEIYSEILDDMNPDDEKIPYLYIEYADALIKSSDMFFIEEISKISGRKGLNLEERKGAEDDLEIAWNLLEICKNAFSILKDYSMLARTRFLQGEICLLNNNFRDGLHEFIECVAVMDKIYEKDCFRYADVYQSLANCYEFLEDFEKSREYYEKAIGIYKAEQERKESEEEKNEICDVITELLQKTTELKYKKEKLENTDLPDDPESDDVFIDINACKRNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARH7</accession>
    <name>I7ARH7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CCAAT box binding factor subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NFYC/HAP5 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264474.1">
        <property type="nucleotide sequence ID" value="XM_009266199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARH7"/>
    <dbReference type="GeneID" id="20521276"/>
    <dbReference type="KEGG" id="ero:EROM_050440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050440"/>
    <dbReference type="HOGENOM" id="CLU_105950_0_0_1"/>
    <dbReference type="OrthoDB" id="1272441at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22908">
        <property type="entry name" value="HFD_NFYC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000062">
        <property type="entry name" value="Nuclear transcription factor Y subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050568">
        <property type="entry name" value="Transcr_DNA_Rep_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252:SF54">
        <property type="entry name" value="CHROMATIN ACCESSIBILITY COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10252">
        <property type="entry name" value="HISTONE-LIKE TRANSCRIPTION FACTOR CCAAT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="7">
        <location>
            <begin position="38"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="218" mass="25602" checksum="852305CC60E3A08D" modified="2012-10-03" version="1">MIGRMRNEYGLLDERISKFWHQTFKAAVEERILLKDLNLPLARIKRLMKIEEGVRMVASEVPVLFSMITEKFIEELTLRAWINTEENKRRILQKSDLTAAVKTSEMFDFLVYIVPRNDLLHPFNHLVPNKVHHGEEEFAPRVGDGYTKHRLSERQMMENMAEKMGTTHHQGFYSQDHRIGNEIPESITNPHVDDYRRGFDISFNRDMPTDMTPGGSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ART4</accession>
    <name>I7ART4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264589.1">
        <property type="nucleotide sequence ID" value="XM_009266314.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ART4"/>
    <dbReference type="GeneID" id="20521394"/>
    <dbReference type="KEGG" id="ero:EROM_051620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051620"/>
    <dbReference type="HOGENOM" id="CLU_1415146_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707606" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22724" checksum="AEF430B9E301FC32" modified="2012-10-03" version="1">MNVNLILSNILGSILLGLAKARSNENESNPSISANSDESNGDGVNPLIPGSETKYPGIVSEVKRIQALCSDTLANLQLYITASEEGRISLDGDELDTIKYFCNQLTELVKMWGNLSGLLEQKEISLKEIKSIVINIISLETEVIKHAGTFNVKRYLTEEFLQHIDEYITNHRGNLDYLISKIVNSSIREPLFIISPQDTGNDAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS36</accession>
    <name>I7AS36_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">General transcription factor TFIIB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_061050</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264694.1">
        <property type="nucleotide sequence ID" value="XM_009266419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS36"/>
    <dbReference type="GeneID" id="20521501"/>
    <dbReference type="KEGG" id="ero:EROM_061050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061050"/>
    <dbReference type="HOGENOM" id="CLU_043736_1_1_1"/>
    <dbReference type="OrthoDB" id="25790at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20551">
        <property type="entry name" value="CYCLIN_TFIIB_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20552">
        <property type="entry name" value="CYCLIN_TFIIB_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000019">
        <property type="entry name" value="transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="15">
        <location>
            <begin position="10"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00056616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="312" mass="35124" checksum="E584BDB502919E4B" modified="2012-10-03" version="1">MKTLPKFKPFVQACSDCGETQNIIEDYKNGYHVCGRCGCIVGNRIIDEGSEWRSFGDSNKVDPCRIGSASNPYLESEQLDTMISTGGGVNSYMLSKIQMKNSMRGPERALKHGMNLITAFCERSNLSRTIIDRAHYIFKNIEERKLLKGKNVEGIVAACMYIACRQEECPRTFKEISVMTAVQKREIGRCFKLISPHLERMATMSTENIIARFCSDLNLSIKIQKIATEIAKAAHELGCLAGKSPDSIAAAVIYMVTNLFPEEKKIQKDIQFVTNVTEVTIKNTYKELVAFKYDIIPENMVNRESIDKLPGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASR5</accession>
    <name>I7ASR5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264984.1">
        <property type="nucleotide sequence ID" value="XM_009266709.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASR5"/>
    <dbReference type="GeneID" id="20521804"/>
    <dbReference type="KEGG" id="ero:EROM_080680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080680"/>
    <dbReference type="HOGENOM" id="CLU_073630_0_0_1"/>
    <dbReference type="OrthoDB" id="2189509at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031537">
        <property type="entry name" value="DUF5092"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17010">
        <property type="entry name" value="DUF5092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40705" checksum="6F394345AB4FF6DE" modified="2012-10-03" version="1">MAKETPDLYERSKGIPSKIRIVGGPNNLAYGEGDELDVLDANTVETFTLEEFKAIVLDCRSKNKDFILARVTTPDPDDQSIIYNFYYAASELNRILFRFESDRRLLHRMKVRNPLNNMYILGQVYYYKIPPQEVDRALIEYFFIETKVEGKVTKKAFSQVFRHKIEEASEDGKRCHKSRDSLDANDRSLKINSVEENPREIIEKIQKGIISIPKAIPPERSVLYSANYFASDDDFLMQPEVREYFRRNTLDADDDFLFEIDRTQNDFFALLETASEDENEEVLGWKRVLTAHISMLVTLLTVCLVLGVGPQMVLVALPLAVVLIFSFIGSIFYILFCRRNTFDTLAVNSIEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATB0</accession>
    <name>I7ATB0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mRNA guanylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_090400</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:67012"/>
            <dbReference type="Rhea" id="RHEA-COMP:17165"/>
            <dbReference type="Rhea" id="RHEA-COMP:17166"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:167616"/>
            <dbReference type="ChEBI" id="CHEBI:167617"/>
            <dbReference type="EC" id="2.7.7.50"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:67013"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.7.50" evidence="2"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265154.1">
        <property type="nucleotide sequence ID" value="XM_009266879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATB0"/>
    <dbReference type="GeneID" id="20521978"/>
    <dbReference type="KEGG" id="ero:EROM_090400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090400"/>
    <dbReference type="HOGENOM" id="CLU_021710_0_1_1"/>
    <dbReference type="OrthoDB" id="200924at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004484">
        <property type="term" value="F:mRNA guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001339">
        <property type="entry name" value="mRNA_cap_enzyme_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013846">
        <property type="entry name" value="mRNA_cap_enzyme_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051029">
        <property type="entry name" value="mRNA_Capping_Enz/RNA_Phosphat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10367:SF17">
        <property type="entry name" value="MRNA-CAPPING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03919">
        <property type="entry name" value="mRNA_cap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01331">
        <property type="entry name" value="mRNA_cap_enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="mRNA capping enzyme adenylation" evidence="11">
        <location>
            <begin position="5"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="mRNA capping enzyme C-terminal" evidence="12">
        <location>
            <begin position="198"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="288" mass="33879" checksum="1D25926BA1863832" modified="2012-10-03" version="1">MPRGYFYDRKNDFYELGINFPFSSQVLVDGEVFLEDGTTPTYAIFDCLIYEGTSQVSKNLYKRLGYAQMFVEKMNENMEKIKVLRKEGEDGFGEKRVPIEAGTQGLDRTHIHFYTKEMMKSYGFWEIYKKIPELKHGNDGLIFTPANEPYSVGRRGTVLKWKPSSLNTIDFKAVKNERLNYVYDLVCSGKKGKDVVFDHFFCEDEEIDGKIGEFTYDSDGYYWDFDELVLKRGGWKLYRIRTDKDTPNNIKIVCNILESLKDNLTIEKLCTFYNIMRENSKNRERMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATF2</accession>
    <name>I7ATF2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265214.1">
        <property type="nucleotide sequence ID" value="XM_009266939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATF2"/>
    <dbReference type="GeneID" id="20564325"/>
    <dbReference type="KEGG" id="ero:EROM_091010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091010"/>
    <dbReference type="HOGENOM" id="CLU_420345_0_0_1"/>
    <dbReference type="OrthoDB" id="2193202at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="498"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="596"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="410"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="498"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="651" mass="72898" checksum="26C5C4F8CB8167AB" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATF4</accession>
    <name>I7ATF4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein ARV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_091060</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Mediator of sterol homeostasis involved in sterol uptake, trafficking and distribution into membranes.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Regulates also the sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ARV1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265219.1">
        <property type="nucleotide sequence ID" value="XM_009266944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATF4"/>
    <dbReference type="GeneID" id="20564330"/>
    <dbReference type="KEGG" id="ero:EROM_091060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091060"/>
    <dbReference type="HOGENOM" id="CLU_1250668_0_0_1"/>
    <dbReference type="OrthoDB" id="2192830at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097036">
        <property type="term" value="P:regulation of plasma membrane sterol distribution"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007290">
        <property type="entry name" value="Arv1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467">
        <property type="entry name" value="ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14467:SF0">
        <property type="entry name" value="PROTEIN ARV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04161">
        <property type="entry name" value="Arv1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 10" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0746">Sphingolipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="221" mass="25459" checksum="CF622C71FDEEEDAD" modified="2012-10-03" version="1">MHVCIECGSRAKQLFLKNSSAKQISKCLVCCKKMDRYFELNGMMKLIDLLLLKRRIFRHYLFNNKKDFTRSVLLMLTVKILTAPILQHHKTLRLFFSGEGLDNVSIAAIPIICRDVIESLMETLLFLVLVYAVFHRCIGFIKLSSALLLSSFYYLFIFIMIVWKYQWEEYLLVIEFLCVASNSIVVSEICQVRNETAVGYLYGCKIATGLLCKGILRSIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATM3</accession>
    <name>I7ATM3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Signal recognition particle receptor subunit alpha homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Docking protein alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_100030</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Peripheral membrane protein</topology>
            <orientation evidence="7">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265319.1">
        <property type="nucleotide sequence ID" value="XM_009267044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATM3"/>
    <dbReference type="GeneID" id="20564433"/>
    <dbReference type="KEGG" id="ero:EROM_100030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100030"/>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_1"/>
    <dbReference type="OrthoDB" id="1727884at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17876">
        <property type="entry name" value="SRalpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000566">
        <property type="entry name" value="Signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="10">
        <location>
            <begin position="396"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="424" mass="47134" checksum="C271D6FC2CBD6401" modified="2012-10-03" version="1">MGYFCVFNKRGLVIHEEGKAPDHLNQIIASLDTSKLIGLRKIRDQVMEYEIRGPIVYLSLSRTPNLKAIIDDYLGGNKPKIVEGKKKTRNSIKGRKWEDAGNEEELNYSTSSSGISNLVEVIRKATVQKAFGLFKDEIQVSELKDKMTTHLISKNVSSQITKTLVDNITMELAEEGLESISEKAFKQKMSNVLSRLIPSVDHERMLDKIKRHKGVFSICFVGVNGVGKSTSLAKICYWLLQNKFRVYIAACDTFRAGAIEQLRTHVERFKLGGHLVGFYEKGYGKDDASVARCAIQEAQHEGYDVILIDTAGRMHNKKNLMASLTKLIKINNPDHIIYVGEALVGSDSLDHIREFNKAIGDAGQSRKIDSILLTKVDTVDNKIGQILNMAFSSHAPILFLGVGQSNSDLSNIGSEDIVNSLMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATT9</accession>
    <name>I7ATT9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_100860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265399.1">
        <property type="nucleotide sequence ID" value="XM_009267124.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATT9"/>
    <dbReference type="GeneID" id="20564516"/>
    <dbReference type="KEGG" id="ero:EROM_100860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100860"/>
    <dbReference type="HOGENOM" id="CLU_847578_0_0_1"/>
    <dbReference type="OrthoDB" id="2190224at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="304" mass="35863" checksum="EEEF59D0B8FDAAB0" modified="2012-10-03" version="1">MMSLISPINSLFEMEEIERDREAVRRMKKFDKETMEAAHSESLKSKRISYIPNLVSMSTSKHAKKSTKPGVLSLKIRSMSTRNILFAVSESFRNIDKKIIRKLERIKEELIKRDDLFECIVDHIESMDVIEDELFSWYPGLKTSDILSFFLDLMPNLLERYKKYFVKSLVLHQDPKKKILNVLRDRLHKNLQCFDIIERDLELFSKFSKNLSPEGRIITSSYWCEDDDKCEDALRLFPQLEDRVCLSPDVCVELFHPLSHAEVQINGRDLVVSFVQLNDLLTRNSRSLDFWMREGIVDKDWVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU69</accession>
    <name>I7AU69_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-directed RNA polymerase I and III subunit AC19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_110450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265524.1">
        <property type="nucleotide sequence ID" value="XM_009267249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU69"/>
    <dbReference type="GeneID" id="20564644"/>
    <dbReference type="KEGG" id="ero:EROM_110450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110450"/>
    <dbReference type="HOGENOM" id="CLU_090381_4_2_1"/>
    <dbReference type="OrthoDB" id="510325at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07029">
        <property type="entry name" value="RNAP_I_III_AC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033898">
        <property type="entry name" value="RNAP_AC19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="4">
        <location>
            <begin position="14"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="100" mass="11385" checksum="C83353CDD33DAAE0" modified="2012-10-03" version="1">MNKEDCIKILNDQTIQVNKEDHTIMNPLRWCISKNFVGEKVELVGYTIPHPSDDVSNITIQFSSESSQTPPNLLKKMIEGLECMEAIGNKMLSEIEKFKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU83</accession>
    <name>I7AU83_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Class 2 transcription repressor NC2 subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_110600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265539.1">
        <property type="nucleotide sequence ID" value="XM_009267264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU83"/>
    <dbReference type="GeneID" id="20564659"/>
    <dbReference type="KEGG" id="ero:EROM_110600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110600"/>
    <dbReference type="HOGENOM" id="CLU_066247_11_3_1"/>
    <dbReference type="OrthoDB" id="601405at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017054">
        <property type="term" value="C:negative cofactor 2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22905">
        <property type="entry name" value="HFD_Dr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042225">
        <property type="entry name" value="Ncb2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46138:SF1">
        <property type="entry name" value="PROTEIN DR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="3">
        <location>
            <begin position="9"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="145" mass="16832" checksum="A09AA1F8E7E1453F" modified="2012-10-03" version="1">MEKCDDENTLPKATVDKMVSSMLPKNSVVPKESKEIFQSACVYFLNMLTLEANKACEEEKKKTISYEHVYKALKNLGFENYIDDCTKEHGNYENYIKQKPSKIDKFKDSGLSMEELHNQQIKLFQNAKLQFERSFEDDHCGDNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUB4</accession>
    <name>I7AUB4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AAA ATPase proteasome regulatory subunit YTA6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_110900</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265569.1">
        <property type="nucleotide sequence ID" value="XM_009267294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUB4"/>
    <dbReference type="GeneID" id="20564689"/>
    <dbReference type="KEGG" id="ero:EROM_110900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110900"/>
    <dbReference type="HOGENOM" id="CLU_000688_21_0_1"/>
    <dbReference type="OrthoDB" id="10251136at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000022">
        <property type="entry name" value="Fidgetin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000093">
        <property type="entry name" value="Fidgetin-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050304">
        <property type="entry name" value="MT-severing_AAA_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23074:SF17">
        <property type="entry name" value="FIDGETIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="186"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="425" mass="48223" checksum="75A2D4A0FE0A8EF4" modified="2012-10-03" version="1">MENSLYELQKILEKRGVPGFPSLLPHELRDLETAHCLEKLCEEMLCEVSRNNNLMPILSFITGLPVEVDIEEIERLLAGKKPVKGDFFKIGHCPRSLPLEKDEYPGFVTGGGNKIIKKDQKDKNEVDGECNVESYIVDRIRNEILEKTVDVKWDDIIGLKDVKKAINEIVLWPMLRPDLFTGLRGPPKGLLLFGPPGTGKTMIGKCIASQCKATFFSISASSLTSKWVGEGEKMVRALFYLARSMQPSVVFIDEIDSLLSQRSDNENEGSRRIKTEFLVQFDGAGTSDGDRILVIGATNRPHEIDEAARRRLVKRIYVPLPENLGRRQMVEHLIKEYRNTLEHADLNEVAKMTEGYSGSDIFNLCREASLEPLREIDDIEDFKSEDTRPISLEDFRKATRQIKKSVSERDLEIYSDWNTKFGSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUC3</accession>
    <name>I7AUC3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Translation elongation factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265579.1">
        <property type="nucleotide sequence ID" value="XM_009267304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUC3"/>
    <dbReference type="GeneID" id="20564699"/>
    <dbReference type="KEGG" id="ero:EROM_111000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111000"/>
    <dbReference type="HOGENOM" id="CLU_011666_0_0_1"/>
    <dbReference type="OrthoDB" id="364892at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="4"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="678" mass="76799" checksum="298CEEADF1978D27" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGT0</accession>
    <name>I6ZGT0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_010490</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263890.1">
        <property type="nucleotide sequence ID" value="XM_009265615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGT0"/>
    <dbReference type="GeneID" id="20520675"/>
    <dbReference type="KEGG" id="ero:EROM_010490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010490"/>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_1"/>
    <dbReference type="OrthoDB" id="1045173at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="10">
        <location>
            <begin position="11"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="429" mass="46722" checksum="73BEFBF10237A5AE" modified="2012-10-03" version="1">MKDVSEIMKKQATLNIGTIGHVAHGKSTIVKAISGISTIKFKAELERNITIKLGYANAKIYKCDSKCIRPNCYQSFGSSNPDQLPCKKCGGTLKLVRHVSFVDCPGHDVLMATMLNGTAIMDSVLLLIAANEPCPQPQTTEHLFAVEIMDLKKVLVVQNKIDLVSREQALEQYDQIQKFLRTSNVSGPVIPTAAQVGVNIPALLDFIVNYIPQPARDSTAHPKMVVIRSFDVNRPGTKVGDMSGGVIGGSLVTGMLRVGDRIEIRPGLVMKRNNRFVCRPFVSEIVSLKAESTDLEEAYPGGLIGVGTTMDPSFCKADKLVGQVMGKLGFLPSIFHKITVEYSLFPKTTVQGSFKLKEGEHVLLNIGSTTTGSVIGGIGETRGEFDLVKPACCEIGERIAISRKINNHWRLIGHGEIKDGVCVEPEYTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGV0</accession>
    <name>I6ZGV0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263910.1">
        <property type="nucleotide sequence ID" value="XM_009265635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGV0"/>
    <dbReference type="GeneID" id="20520696"/>
    <dbReference type="KEGG" id="ero:EROM_010690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010690"/>
    <dbReference type="HOGENOM" id="CLU_1855258_0_0_1"/>
    <dbReference type="OrthoDB" id="2194766at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707273" evidence="1">
        <location>
            <begin position="26"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="141" mass="15547" checksum="C3756DE2D5DF36E2" modified="2012-10-03" version="1">MDILRKCASLQLACMLMGMIGMGTATKPGESSSSSIYGVSNERKIINNYFSDDQIEKFLEWISKIPNIIKENSSEIKVASGLIKGSRENLSGPVRAIGDVFIRILGTNVNDVKFFKDFFILVLVKLINIPDIIEEVSSALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH70</accession>
    <name>I6ZH70_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4 14">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_020680</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the signal peptidase complex (SPC) composed of a catalytic subunit SEC11 and three accessory subunits SPC1, SPC2 and SPC3. The complex induces a local thinning of the ER membrane which is used to measure the length of the signal peptide (SP) h-region of protein substrates. This ensures the selectivity of the complex towards h-regions shorter than 18-20 amino acids. SPC associates with the translocon complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the peptidase S26B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 14"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264040.1">
        <property type="nucleotide sequence ID" value="XM_009265765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH70"/>
    <dbReference type="MEROPS" id="S26.010"/>
    <dbReference type="GeneID" id="20520828"/>
    <dbReference type="KEGG" id="ero:EROM_020680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020680"/>
    <dbReference type="HOGENOM" id="CLU_089996_0_0_1"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019758">
        <property type="entry name" value="Pept_S26A_signal_pept_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00761">
        <property type="entry name" value="SPASE_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="175" mass="20081" checksum="8520AD31F66FAD53" modified="2012-10-03" version="1">MLEYIISEKDIRMLKRMSIRQKLVQFVNAAYSVMGTYMIWKMFSILLNNDSPIVVVLSESMSPGFERGDILWLANKDFSVGDMTVFKFGKEIPCVHRCIKQFGGRYLTKGDNNLDDDVSLYPKGRNYLTRNEVKSIVVGYIPYFGWINLWINTIPGVKFAILAAVGLSVLFTREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHA9</accession>
    <name>I6ZHA9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NTF2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_021080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264080.1">
        <property type="nucleotide sequence ID" value="XM_009265805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHA9"/>
    <dbReference type="GeneID" id="20520870"/>
    <dbReference type="KEGG" id="ero:EROM_021080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021080"/>
    <dbReference type="HOGENOM" id="CLU_1315757_0_0_1"/>
    <dbReference type="OrthoDB" id="339151at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NTF2" evidence="1">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="219" mass="24894" checksum="D5EE9AC843FAD259" modified="2012-10-03" version="1">MVCQKDANFCSPMTSIPKSNEVVFIRNYYSMLCNRPSLIHQYYMKDSQLTISKEMDKPEICTEDFGKYIKSKISNPISKVLISHLSCQKIDDLRSIINVIGQFVYNDISQVRITHQFIVSKSDSTLYIKNEILTFLDEEVIYETESDAKRVIVVSCGKNGMPQAISAISEFGKVRSMKSENDGKVVMTLNSMEDVENIKRNIAKIVSQGYKLEFGGLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHC5</accession>
    <name>I6ZHC5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trehalase</fullName>
            <ecNumber evidence="4">3.2.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Alpha-trehalose glucohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_021280</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:32675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15903"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:17925"/>
            <dbReference type="EC" id="3.2.1.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 37 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.28" evidence="4"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264100.1">
        <property type="nucleotide sequence ID" value="XM_009265825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHC5"/>
    <dbReference type="GeneID" id="20520890"/>
    <dbReference type="KEGG" id="ero:EROM_021280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021280"/>
    <dbReference type="HOGENOM" id="CLU_006451_4_0_1"/>
    <dbReference type="OrthoDB" id="3542292at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004555">
        <property type="term" value="F:alpha,alpha-trehalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005993">
        <property type="term" value="P:trehalose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001661">
        <property type="entry name" value="Glyco_hydro_37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018232">
        <property type="entry name" value="Glyco_hydro_37_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23403:SF1">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01204">
        <property type="entry name" value="Trehalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00744">
        <property type="entry name" value="GLHYDRLASE37"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00928">
        <property type="entry name" value="TREHALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="646" mass="74611" checksum="E4C9D9801AAB69D2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHS0</accession>
    <name>I6ZHS0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_031210</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264240.1">
        <property type="nucleotide sequence ID" value="XM_009265965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHS0"/>
    <dbReference type="GeneID" id="20521035"/>
    <dbReference type="KEGG" id="ero:EROM_031210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031210"/>
    <dbReference type="HOGENOM" id="CLU_335237_0_0_1"/>
    <dbReference type="OrthoDB" id="2192736at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="850" mass="97013" checksum="12E0BFE67C8930B1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHS3</accession>
    <name>I6ZHS3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-dependent NAD(P)HX dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_031260</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="7"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264245.1">
        <property type="nucleotide sequence ID" value="XM_009265970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHS3"/>
    <dbReference type="GeneID" id="20521040"/>
    <dbReference type="KEGG" id="ero:EROM_031260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031260"/>
    <dbReference type="HOGENOM" id="CLU_1086541_0_0_1"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="8">
        <location>
            <begin position="7"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="152"/>
            <end position="158"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="171"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="278" mass="30749" checksum="279E6590F71626C8" modified="2012-10-03" version="1">MNREFSETISSIKSEIRKSTSDKKGSSGTILIIGGSKYYTGAPYFASLAAFHSGSELVYIFSEPEATVSLKTLLPESIVCGIEYQEWILKKVSCCIMGPGLGRPSEETCKEITRILSYLGRRDIPIVIDGDGIRLASKLDIGDLKTVIITPNTNEQKYIGEFEKRIFYVLKGSNDVILWKDKEVKINNEGCPKRIGGQGDILAGTIASLVSKCKPPSSDKEVFGSIILGCVMVRKAGRLAYEKYWKSLITRDILEMLKESFRAGVEDSPELLALQSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHW9</accession>
    <name>I6ZHW9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Exocyst complex component Sec10-like alpha-helical bundle domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_040390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264305.1">
        <property type="nucleotide sequence ID" value="XM_009266030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHW9"/>
    <dbReference type="GeneID" id="20521103"/>
    <dbReference type="KEGG" id="ero:EROM_040390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040390"/>
    <dbReference type="HOGENOM" id="CLU_028905_0_0_1"/>
    <dbReference type="OrthoDB" id="2196302at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="1">
        <location>
            <begin position="157"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="631" mass="73424" checksum="A22E3B91E82FD217" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJW7</accession>
    <name>I6ZJW7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoinositide polyphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265055.1">
        <property type="nucleotide sequence ID" value="XM_009266780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJW7"/>
    <dbReference type="GeneID" id="20521877"/>
    <dbReference type="KEGG" id="ero:EROM_081420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081420"/>
    <dbReference type="HOGENOM" id="CLU_003016_7_4_1"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAC" evidence="1">
        <location>
            <begin position="120"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="453" mass="52321" checksum="3861CD92DCCC1A09" modified="2012-10-03" version="1">MARLVYSESSKRISWETESGVATLSYTGTVSKKPDLKQIEFQTILGILRVERNKHHEEFLACVTKSTLVGMYDAVEVYKIDKIRFLNIKGPGRADEVMSQVKGFVQSHDFYYFFGSLFIEDEFVWNKHMKANLLKYFGPSSYMWVLSHRPGREPLNLNISTLFCGFFAAEAFKASGDFYHMKLLSLISSNRVGTRYLCRGVDSEGNASLFVKTHFQTKRNNNTIFDFTTIRGSVPIFWSQTEHGISTKINIYGDDESVREAFTKHFKKLRREYGKVHVINLLGEKKYEKQLTLHFNRLLNLENIPYTTFDLDAHVKNYDDLKFLLYFKLQGIEEEGVTFRVNCLDCLDRTNVAQSLICMFYLEKAVSNGDVLKKMQECWANNGNSLSNLYTGSDAMKKELALKGRRSFIGYMDDFVISATRLINGRFTDKQKHSIINTLLEKKEDVEEVRASY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJX3</accession>
    <name>I6ZJX3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine/threonine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_081470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265060.1">
        <property type="nucleotide sequence ID" value="XM_009266785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJX3"/>
    <dbReference type="GeneID" id="20521882"/>
    <dbReference type="KEGG" id="ero:EROM_081470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081470"/>
    <dbReference type="HOGENOM" id="CLU_581437_0_0_1"/>
    <dbReference type="OrthoDB" id="193931at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="7">
        <location>
            <begin position="10"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="470" mass="54571" checksum="7318282F89BF07B1" modified="2012-10-03" version="1">MYLQKKFLNFNITGLLGSGSYGDVVSATDNITGMRVAMKFIRKDVDRWKHSLELKIQRLLNHRSVCKLYGHVNTSRNLVLVLELARGIDLHKYIRRNGRLEEGYARTLFVQMLHGVDYLHTHSIVHRDLKLENIIVDGETVKICDFGLSTFYDRSSVLRDYCGTPQCAPPEIMRGIPYVGPEIDIWCLGIILYAMVHGRLPFEEEEIGLSSRHAIISRMSVDESLSNELKDLIRRIIEPDKSIRIEMDDLMRHPWVNVTREDYRKSIVFIDDNVVESLVGMGFKKEDVLRNIPNKNSKEYSAYCLVSKDPLSLSRHQVVPGNIRQCHPYNIVDLNFLIDEETISNHQRRMWKHEILRRMSPVRNGCLLSLLWGKRKMYLVKRDINLNLKMSKSLIEHSLATFPKITFRKKSRYVVHHTSGLVVSIELTEKFPFTVCTFILTGGNKIEFVNFVVDFIRFSNQENKEACIWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJY4</accession>
    <name>I6ZJY4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_081570</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265070.1">
        <property type="nucleotide sequence ID" value="XM_009266795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJY4"/>
    <dbReference type="GeneID" id="20521892"/>
    <dbReference type="KEGG" id="ero:EROM_081570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081570"/>
    <dbReference type="HOGENOM" id="CLU_084051_0_0_1"/>
    <dbReference type="OrthoDB" id="10258869at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="219" mass="24984" checksum="5559001D9E75B520" modified="2012-10-03" version="1">MGRRPGRCYRYLSKKAYPKSRFNRGVPDSKIQIFDLGRRKAGVLEFPLLVNCISNERENLSAEALEAARICANKYMVKHAGKDNFHLRVRVYPFHVLRINKMLSCAGADRLQTGMRGSFGKPYGRAARVVFNQPILSIRTKESFKDVAVEALRRAKNKFPGHQEIQVSSKFGFTNMLNDEFNNLRENGRIILRGGSFSVIREKGSIDNFKRNLEQAANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKB4</accession>
    <name>I6ZKB4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">18S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="6">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TSR3</name>
        <name evidence="9" type="ordered locus">EROM_090920</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 1191 (Psi1191) in 18S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>N(1)-methylpseudouridine(1191) in yeast 18S rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine(1191) in yeast 18S rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63300"/>
            <dbReference type="Rhea" id="RHEA-COMP:13852"/>
            <dbReference type="Rhea" id="RHEA-COMP:16309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="6"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265205.1">
        <property type="nucleotide sequence ID" value="XM_009266930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKB4"/>
    <dbReference type="GeneID" id="20564316"/>
    <dbReference type="KEGG" id="ero:EROM_090920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090920"/>
    <dbReference type="HOGENOM" id="CLU_035060_4_2_1"/>
    <dbReference type="OrthoDB" id="10262062at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 6" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="7">
        <location>
            <begin position="39"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="172" mass="19626" checksum="2997A5280D485BA0" modified="2012-10-03" version="1">MRKIIYEFEQCDPKKCSGKRLIKQNKVQPIKKNKSFGGVVLSPDADQSISPADRDIIERFGIGLIDCSWAQLDSVDFKRLPRKHNRLLPFMVAANPVNYGKPFKLNCAEALSASLYICGFKEDAYEILEGFNYGNEFYKLNGDILDEYAKCTSSIEVVEAQNRFLKSQARRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKJ2</accession>
    <name>I6ZKJ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane traffic regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265310.1">
        <property type="nucleotide sequence ID" value="XM_009267035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKJ2"/>
    <dbReference type="GeneID" id="20564423"/>
    <dbReference type="KEGG" id="ero:EROM_091990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091990"/>
    <dbReference type="HOGENOM" id="CLU_133395_0_0_1"/>
    <dbReference type="OrthoDB" id="10009287at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004345">
        <property type="entry name" value="TB2_DP1_HVA22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03134">
        <property type="entry name" value="TB2_DP1_HVA22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="179" mass="20579" checksum="5FADCC89F1763561" modified="2012-10-03" version="1">MDCLRKQAERIPILDAIEKRMNIRKEYALLGISFLCLVIIMATSLGPLVTSTIGIIVPLQETFVILRQVNPKKEETKHMLVFWMIFGVLTSLDAYYNTIIGFIPLWYTIKFFFLLWAGPLKFRGGIIIYDNILTRIPESWYREEGGIENAVKKATDAVKSVAENEFNKKDVIENSKKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKZ6</accession>
    <name>I6ZKZ6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA polymerase II transcription</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_110090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265490.1">
        <property type="nucleotide sequence ID" value="XM_009267215.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKZ6"/>
    <dbReference type="GeneID" id="20564608"/>
    <dbReference type="KEGG" id="ero:EROM_110090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110090"/>
    <dbReference type="HOGENOM" id="CLU_048466_2_0_1"/>
    <dbReference type="OrthoDB" id="5963at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015877">
        <property type="entry name" value="MAT1_centre"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12683:SF13">
        <property type="entry name" value="CDK-ACTIVATING KINASE ASSEMBLY FACTOR MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06391">
        <property type="entry name" value="MAT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17121">
        <property type="entry name" value="zf-C3HC4_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="223" mass="25798" checksum="CEAF4A4B2A358145" modified="2012-10-03" version="1">MEERRCPVCKSDIYLNPDIVIYISPCFHKMCESCMLRIFNRGQAPCPECGTMLRKINYIKPTFEDITVEKECKVRKVLMKVFGREEEEFADVAKYNDYLEKFEEFVFEVLNIKGESLVKERIKEIQDMGEGSILNPIPKTIPGKSVLQTRKRQSHDLKEERSKIQKIAITTDYFDPEELPRIISNPAREIYLPKGFLNPSLPGGLSYDIIISFAISSLYNPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL90</accession>
    <name>I6ZL90_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Kinetochore protein SPC25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_111110</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the NDC80 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Chromosome</location>
            <location evidence="9">Centromere</location>
            <location evidence="9">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SPC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265590.1">
        <property type="nucleotide sequence ID" value="XM_009267315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL90"/>
    <dbReference type="GeneID" id="20564710"/>
    <dbReference type="KEGG" id="ero:EROM_111110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111110"/>
    <dbReference type="HOGENOM" id="CLU_1180210_0_0_1"/>
    <dbReference type="OrthoDB" id="2188707at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031262">
        <property type="term" value="C:Ndc80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23784">
        <property type="entry name" value="RWD_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.457.50">
        <property type="entry name" value="Chromosome segregation protein Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013255">
        <property type="entry name" value="Spc25_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08234">
        <property type="entry name" value="Spindle_Spc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 9" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 9" id="KW-0137">Centromere</keyword>
    <keyword evidence="2 9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="4 9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromosome segregation protein Spc25 C-terminal" evidence="11">
        <location>
            <begin position="163"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="53"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN84093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="235" mass="27835" checksum="9862FA2067FF9CE2" modified="2012-10-03" version="1">MGMEHTAANAEKIRGVMDGIINDLEGYLEMFRNHARFLRGRISRDKQEGEVLLQTVTSTLNEMRERNERLVDERNALKSRIEQDTKDLKASEDEKHELMNQIRELEMNNLDLKEVFQEKKGIENAFVKKMELIFERNKQREALMEAKAEWFKRYLGMDIIPVRENVIKIVFSRICPEDSVECFVTLDLSMEDAVIDLFPRMYDLEKANGVFRECRSFHDFLAVIRKEFRREYSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS59</accession>
    <name>I6ZS59_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263933.1">
        <property type="nucleotide sequence ID" value="XM_009265658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS59"/>
    <dbReference type="GeneID" id="20520719"/>
    <dbReference type="KEGG" id="ero:EROM_010920"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010920"/>
    <dbReference type="HOGENOM" id="CLU_1343625_0_0_1"/>
    <dbReference type="OrthoDB" id="2192581at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22437" checksum="56B08482987B7FDB" modified="2012-10-03" version="1">MLVFVKNGEVIHTEDLQNVDPLTLETFEEILASTGSVVFCILVCESTRHVYLASSIVSMRYTVSGAIRIYKLKDPSTRNEVKDILYFEVVRNPLYLRIFKDIQKSNPLRRKGNTMDSLGSSAGDFSQVGPVVKAVFIGNELDLVYNTDLQKKVFRNGEKRGSFASTVIGGVVFFLSFILLFFFMVCFFYILLKFVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSB6</accession>
    <name>I6ZSB6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Microsporidial 8TM transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_020260</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263998.1">
        <property type="nucleotide sequence ID" value="XM_009265723.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSB6"/>
    <dbReference type="GeneID" id="20520784"/>
    <dbReference type="KEGG" id="ero:EROM_020260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020260"/>
    <dbReference type="HOGENOM" id="CLU_994079_0_0_1"/>
    <dbReference type="OrthoDB" id="2190933at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031497">
        <property type="entry name" value="8TM_micro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17028">
        <property type="entry name" value="8TM_micro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707407" description="Microsporidial 8TM transmembrane domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Microsporidial 8TM transmembrane" evidence="3">
        <location>
            <begin position="17"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="280" mass="33299" checksum="524BEC06E382F697" modified="2012-10-03" version="1">MSRITKTLMYALILVLRFYDVPSFCYNYHSPETHYQQEFLKPSTIPTVYQIIPRKYFIYVSAICDFTVSRLMNSEKYFIYSSFLPADSISIENLLVILGKAHKDATSFLLTHLSVFYAPLLTRSSSYVLSYIYPAYRKDYENSYRPSLSIAWFFHLNIFPQYSRYFYDTFYALMIYIFSLVPEDQRMEIILLFKPSNYKNYLLYIVDSPLFHFYLLIFVVENYIQYLFKIHGIGNPNFLNWSYLAFTCLFIFERRGGALISQKECTKKKQEKASDTLPQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSC6</accession>
    <name>I6ZSC6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIA subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">General transcription factor IIA subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Transcription initiation factor IIA small chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_020360</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIA subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264008.1">
        <property type="nucleotide sequence ID" value="XM_009265733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSC6"/>
    <dbReference type="GeneID" id="20520794"/>
    <dbReference type="KEGG" id="ero:EROM_020360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020360"/>
    <dbReference type="HOGENOM" id="CLU_112964_4_0_1"/>
    <dbReference type="OrthoDB" id="586585at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005672">
        <property type="term" value="C:transcription factor TFIIA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10014">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.18.10">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.190">
        <property type="entry name" value="Transcription factor IIA gamma subunit, alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009083">
        <property type="entry name" value="TFIIA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009088">
        <property type="entry name" value="TFIIA_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003194">
        <property type="entry name" value="TFIIA_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015871">
        <property type="entry name" value="TFIIA_gsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015872">
        <property type="entry name" value="TFIIA_gsu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10966">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02751">
        <property type="entry name" value="TFIIA_gamma_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02268">
        <property type="entry name" value="TFIIA_gamma_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47396">
        <property type="entry name" value="Transcription factor IIA (TFIIA), alpha-helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50784">
        <property type="entry name" value="Transcription factor IIA (TFIIA), beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIA gamma subunit C-terminal" evidence="11">
        <location>
            <begin position="58"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="113" mass="13125" checksum="21D4FD39069B7660" modified="2012-10-03" version="1">MYEFYRQLMVGKALEKIIDEKVNSNVLTPNQGKYILEAFDLSVPIVFNKSVTNSMSFKGKVDFYNHVDGVWRFQTKDFVMSLNNEYFRAGDVNIVACDADINMDAGRRRRRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSJ3</accession>
    <name>I6ZSJ3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hsp90-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_021010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264073.1">
        <property type="nucleotide sequence ID" value="XM_009265798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSJ3"/>
    <dbReference type="GeneID" id="20520863"/>
    <dbReference type="KEGG" id="ero:EROM_021010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021010"/>
    <dbReference type="HOGENOM" id="CLU_006684_1_3_1"/>
    <dbReference type="OrthoDB" id="28737at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16927">
        <property type="entry name" value="HATPase_Hsp90-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000005">
        <property type="entry name" value="Heat shock protein 90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="entry name" value="Heat shock protein 90, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003555">
        <property type="entry name" value="PRK05218.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="HEAT SHOCK PROTEIN 90 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13589">
        <property type="entry name" value="HATPase_c_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="HSP90 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="6">
        <location>
            <begin position="41"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="141"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002583-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="686" mass="78722" checksum="2F4BF37595EC592C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSR7</accession>
    <name>I6ZSR7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nucleotide-sugar transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_030070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264123.1">
        <property type="nucleotide sequence ID" value="XM_009265848.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSR7"/>
    <dbReference type="GeneID" id="20520914"/>
    <dbReference type="KEGG" id="ero:EROM_030070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030070"/>
    <dbReference type="HOGENOM" id="CLU_585379_0_0_1"/>
    <dbReference type="OrthoDB" id="165382at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF9">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="358"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="467" mass="53069" checksum="E1BE3A84D90C3561" modified="2012-10-03" version="1">MVISCMLGLVAAVQTCLKDSDCLAKSRPDVHSMFCVNNVCQRILSPGKHCTRPTECSSYSFFGPLACTGKCKIESECEYGRDDNLDSMYCCRGIPEGKECNPNRPGSLSGCDMTQSCLMGKKGFYECVPDKSRSWIFGVFLSISGNIGINVGINLQKKSYKQSHIRLFNMNLQTFYAGCFIYGLGKIFGYCSYIFGNQSLMAVLSATGLVSNSIFAPMINEEIFTWKDFCAIFFVFAGTTLIVMNTTTSHKVYTLCELMKMYRRVETLLWFGFIILIIVVLFVFIKYIEINSNWELPDENMTFLRREDVWFDEEGVIMKYTMVLAYVCLSSFIASFTTLSVKSLGEMIDKTIAGDNQFIFLTTYFFIIVLVICTFFQIYWLNRALRHYDALLVIPMFHVTWTLLSILTAGIYFREFEQYTSYQLGVFVGGIGLIFIGSIFLGSRITNKACIKTKNIRTVPKKILKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZST8</accession>
    <name>I6ZST8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_030210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264138.1">
        <property type="nucleotide sequence ID" value="XM_009265863.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZST8"/>
    <dbReference type="GeneID" id="20520929"/>
    <dbReference type="KEGG" id="ero:EROM_030210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030210"/>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_1"/>
    <dbReference type="OrthoDB" id="426865at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="145" mass="16649" checksum="F40D21CED18C984F" modified="2012-10-03" version="1">MSRSVKSVTTQGRKKRAVATCVCTYSGEFSIRINKVPFKLITNTLMIAKLKEIICIVEHANIKDLSFDITCKVGGDVSRVYAVRMAFSKAILAFYGTYYDEWKKQEIQKRLLDFDRFSLVADSRKREPKKFGGPGARARYQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSU4</accession>
    <name>I6ZSU4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264148.1">
        <property type="nucleotide sequence ID" value="XM_009265873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSU4"/>
    <dbReference type="GeneID" id="20520941"/>
    <dbReference type="KEGG" id="ero:EROM_030300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030300"/>
    <dbReference type="HOGENOM" id="CLU_1214748_0_0_1"/>
    <dbReference type="OrthoDB" id="10264655at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="26742" checksum="D9134F1D819C4417" modified="2012-10-03" version="1">MGWRDEITFPTKEVKQFLGVKFITESCVLLSLSYQSRYKALILFYNFNEEIDFAGLCMASVLLASKLEEEVCTLKKVIYVFNYLYTRYESKPTPLTNRLSIRLKEGCILAETQILKSLGFDVSFEDVYGEFIDFLETTDFSPDFIGKAIQVFNTIIQWPEAVSLDPCSLIIATIESLFSRNKRFEDYTRRYRLFQEKRVNLQTYEEVVTTKNISENLITGFFKRQKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSW9</accession>
    <name>I6ZSW9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal RNA-processing protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_030600</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the RNR ribonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264178.1">
        <property type="nucleotide sequence ID" value="XM_009265903.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSW9"/>
    <dbReference type="GeneID" id="20520971"/>
    <dbReference type="KEGG" id="ero:EROM_030600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030600"/>
    <dbReference type="HOGENOM" id="CLU_002333_5_0_1"/>
    <dbReference type="OrthoDB" id="372421at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.700:FF:000001">
        <property type="entry name" value="Exosome complex exonuclease exoribonuclease (Rrp44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041505">
        <property type="entry name" value="Dis3_CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050180">
        <property type="entry name" value="RNR_Ribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033771">
        <property type="entry name" value="Rrp44_CSD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355:SF35">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RRP44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23355">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17849">
        <property type="entry name" value="OB_Dis3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17216">
        <property type="entry name" value="Rrp44_CSD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="RNB" evidence="12">
        <location>
            <begin position="390"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN82681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="835" mass="95547" checksum="DBE8738BDEF44BC8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSX9</accession>
    <name>I6ZSX9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_030750</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264193.1">
        <property type="nucleotide sequence ID" value="XM_009265918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSX9"/>
    <dbReference type="GeneID" id="20520986"/>
    <dbReference type="KEGG" id="ero:EROM_030750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030750"/>
    <dbReference type="HOGENOM" id="CLU_101897_0_0_1"/>
    <dbReference type="OrthoDB" id="2189922at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15959:SF0">
        <property type="entry name" value="SYNTAXIN-18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="27063" checksum="8F049198A85DD116" modified="2012-10-03" version="1">MNRTTEYLKYIDRKTQIPYTSGSESFYDTINSKIEKIEARLRKSLSYRETLKVEEEFATLSLETQQTLDIISVEGSNDLVLHFEGIKRIILMKLQGIERRLRALKDSKIGTSIDLEPERPQVFRNVEEVQVMEEENKKLVEGYNMEGYRLTRRRLLEVEALQDTIFQHLILQDERIDNIIDLTSKAGTYVSGGNEALGRITNSGRFFRRFLFILLLCLSFVLLFLHYYYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT42</accession>
    <name>I6ZT42_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ser/Thr protein phosphatase 2A regulatory subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264283.1">
        <property type="nucleotide sequence ID" value="XM_009266008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT42"/>
    <dbReference type="GeneID" id="20521081"/>
    <dbReference type="KEGG" id="ero:EROM_040170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040170"/>
    <dbReference type="HOGENOM" id="CLU_1111390_0_0_1"/>
    <dbReference type="OrthoDB" id="2193601at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002554">
        <property type="entry name" value="PP2A_B56"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01603">
        <property type="entry name" value="B56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="254" mass="29880" checksum="A7D3BB3DBB194A98" modified="2012-10-03" version="1">MTRTEDVEERKGISELLTCISDYPGCLNTIFFGLANEFISFINNNRTHIGLEEILKVVLVVIRKRSIAYEDMVVFHYQVILGMIRTRFCGESKEISNVIKAICRNYPELIPLTIKHFKKVFGEVWSNTKVVIVHAMGETFSVMGDEMYLCLENEICELIGMSFDSNHHLVIEDMAEILLLNYRSILRHKETFVPKVFEPIYRASQKFWRIEGVNKILRILHAILEMDCRLFERCLKAYNVKRWRNRWKRESSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT72</accession>
    <name>I6ZT72_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Swi5-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_040580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SWI5/SAE3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264323.1">
        <property type="nucleotide sequence ID" value="XM_009266048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT72"/>
    <dbReference type="GeneID" id="20521122"/>
    <dbReference type="KEGG" id="ero:EROM_040580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040580"/>
    <dbReference type="HOGENOM" id="CLU_166973_0_0_1"/>
    <dbReference type="OrthoDB" id="255837at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032798">
        <property type="term" value="C:Swi5-Sfr1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034974">
        <property type="term" value="C:Swi5-Swi2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010772">
        <property type="term" value="P:meiotic DNA recombinase assembly involved in reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000709">
        <property type="term" value="P:meiotic joint molecule formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010760">
        <property type="entry name" value="DNA-repair_Swi5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28529:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN SWI5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07061">
        <property type="entry name" value="Swi5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="13837" checksum="711CF84C5070F679" modified="2012-10-03" version="1">MKLLNDWEKEEVIHKSKIVNFDFLVERNFIDEVKDGFYYLSKDGKTVETELWKKVNHELAEYLDIKDIDKEIKRFIFLLNSYNEIKDIGQELIGKIANLRQTTAKDVHEELGMEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT92</accession>
    <name>I6ZT92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pseudouridine synthase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_040830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264348.1">
        <property type="nucleotide sequence ID" value="XM_009266073.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT92"/>
    <dbReference type="GeneID" id="20521147"/>
    <dbReference type="KEGG" id="ero:EROM_040830"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040830"/>
    <dbReference type="HOGENOM" id="CLU_021971_4_0_1"/>
    <dbReference type="OrthoDB" id="10256309at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02568">
        <property type="entry name" value="PseudoU_synth_PUS1_PUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.660:FF:000002">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041708">
        <property type="entry name" value="PUS1/PUS2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF4">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="6">
        <location>
            <begin position="182"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR641708-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="345" mass="39504" checksum="78C56F96ACFDA623" modified="2012-10-03" version="1">MKIRAGLLVGYDGSRYHGLQFNKELDTIEKRVVECLLQVEAISERNADDPKKIHIKSSSRTDKGVHAALNLISVKIEICITSELVSRLRTLLAKEGIHLYNIVRLTKSTVPSRQAISRTYEYIVPTFFLTKGDLTEEAEALKQKDGEHRIRDTKRDYPENIVESVVGHKSSEDDIKAFGSILQKYIGTKDFHNFTKLNNEKGSKRYIKSITVTDPYVSEGVEYVRISITGQSFLLHQIRKMVLFAVLLCKYSRSSVDPKFNMVFSKQNVHVPKGPSGYLLLDKPVFHKLRRGEGSTEDIGVSDSHREEYKRNVIYPRIHQRKNLIGFFACLDSVRFHKENIGFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTL3</accession>
    <name>I6ZTL3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">serine C-palmitoyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.50</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_050740</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-serine + hexadecanoyl-CoA + H(+) = 3-oxosphinganine + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:14761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:58299"/>
            <dbReference type="EC" id="2.3.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.50" evidence="3"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264503.1">
        <property type="nucleotide sequence ID" value="XM_009266228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTL3"/>
    <dbReference type="GeneID" id="20521306"/>
    <dbReference type="KEGG" id="ero:EROM_050740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050740"/>
    <dbReference type="HOGENOM" id="CLU_015846_7_2_1"/>
    <dbReference type="OrthoDB" id="65434at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017059">
        <property type="term" value="C:serine palmitoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004758">
        <property type="term" value="F:serine C-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046512">
        <property type="term" value="P:sphingosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF3">
        <property type="entry name" value="LD36009P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="9">
        <location>
            <begin position="107"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="475" mass="53656" checksum="589925EA6A5303F5" modified="2012-10-03" version="1">MGENKIPLHLMLVTYLFYLIVVLLGHARDTFGKLLFPKIYKKYKLDNGKVPLYGSFESFFSRRIFRRARDCCERPITGVPGRHVTVLERISEDYNETFRFTGRKLKLLNLGSYNYLGFSNNEGPIVEKVVEVVRHYPLVMTAPTREVGCYDIVRTLEEEMASFLYQEDCMVFSIGYGTNSSNIPVICEAETLILSDELNHASLITGAKISKGVIRVFEHNNMEDLENKLVFNISQGQPLTHRSWKKIIVIVEGIYSMEGTIVNLKKLVELKKKYKFYIFVDEAHSIGALGATGRGVCEYAGVDFKEIDIFMGTFTKSFGGMGGYIAGSKKLITWLRFYSDLSLYGDQIPPFVCSQILESLRCIKFTELGRLKIEKLRSNTVLMRQSLVNAGFFVLGDEFSPVIPILIVCPGKLAEFSRLCMAEGIGVVVVGYPATPILTSRVRLCISASHTTEDVMRAFEVIDRIGERLGMKICK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTM7</accession>
    <name>I6ZTM7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264518.1">
        <property type="nucleotide sequence ID" value="XM_009266243.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTM7"/>
    <dbReference type="GeneID" id="20521321"/>
    <dbReference type="KEGG" id="ero:EROM_050890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050890"/>
    <dbReference type="HOGENOM" id="CLU_1023175_0_0_1"/>
    <dbReference type="OrthoDB" id="2192205at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="279" mass="32675" checksum="223D04A8D33A0F3C" modified="2012-10-03" version="1">MLENLDGFVFRRNVRQEPKARKFFDEGSKEQSIARGNTDGIFSFKFVRSNKRRGDSEEENSGKRAKEKEPDMGVINENEENALNDVSEEISEEDGICSNILIKDEAKKRISISDLHTKLGEDGTLGELVRLCVEYALQKKKIEYGINLERKLLQNEGSIKRRDVEREIEEADRKILWVSQEEEKWNSLKTEVLNSSKVDVREVQRSVRDFGYIENMEEIRQEFVRKFERLEFLKESAKSCISRIREQSEDILGRVLRTICREKEVDTLFLLRTLSKTNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTU6</accession>
    <name>I6ZTU6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264593.1">
        <property type="nucleotide sequence ID" value="XM_009266318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTU6"/>
    <dbReference type="GeneID" id="20521398"/>
    <dbReference type="KEGG" id="ero:EROM_060010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060010"/>
    <dbReference type="HOGENOM" id="CLU_1825259_0_0_1"/>
    <dbReference type="OrthoDB" id="2192540at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="144" mass="17488" checksum="EF859E15C74DB7ED" modified="2012-10-03" version="1">MSLHHQKRIREIRKILSNLESRIDIIESLKRFKDIVLVNEDNDLIVLVIQKVHSILYSSYNIEYIEILLDIIHSEEAFDELFRIIFSFENRPCLPRIINKLRNKNELIIFKYLKELKNNRLFNNLSKEMDKRYNEILDTVDFDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU26</accession>
    <name>I6ZU26_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tubby-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_060470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264638.1">
        <property type="nucleotide sequence ID" value="XM_009266363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU26"/>
    <dbReference type="GeneID" id="20521444"/>
    <dbReference type="KEGG" id="ero:EROM_060470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060470"/>
    <dbReference type="HOGENOM" id="CLU_028236_0_1_1"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF7">
        <property type="entry name" value="PROTEIN KING TUBBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubby C-terminal" evidence="3">
        <location>
            <begin position="183"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="271" mass="30722" checksum="2EBD792422AA046D" modified="2012-10-03" version="1">MERENGYMATSTDHTHNTGDGEQCTLSPLVYEFSYSPSENRVIEYLDNMSDSGVEELHIAVDREHGAGEGISAVLDPYPIGRTSRGRVDVSHGFFNEYLYESHEHGANKLLKAYRVMCGFEICLLEQNNEEKVGYLKSNLFGTKYIFYQGEKAVLEIRYATSIFKRRGPRSFSVMFMGILGSSLNEPVVMKNKEPYYNAETNSYSLNFNGRVTTPSVKNFQLIHPLEPTYVTLTFGKVSRFKYILDYTYPWSGVQAFAIALAALDYKVGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU34</accession>
    <name>I6ZU34_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264648.1">
        <property type="nucleotide sequence ID" value="XM_009266373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU34"/>
    <dbReference type="GeneID" id="20521454"/>
    <dbReference type="KEGG" id="ero:EROM_060570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060570"/>
    <dbReference type="HOGENOM" id="CLU_330650_0_0_1"/>
    <dbReference type="OrthoDB" id="2189784at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="874" mass="103449" checksum="1AC4CA8413342B70" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUB5</accession>
    <name>I6ZUB5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264753.1">
        <property type="nucleotide sequence ID" value="XM_009266478.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUB5"/>
    <dbReference type="GeneID" id="20521563"/>
    <dbReference type="KEGG" id="ero:EROM_070050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070050"/>
    <dbReference type="HOGENOM" id="CLU_1272292_0_0_1"/>
    <dbReference type="OrthoDB" id="2191176at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031502">
        <property type="entry name" value="Zf_ribonucleo"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17026">
        <property type="entry name" value="zf-RRPl_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="219" mass="26285" checksum="EBA6084CB0EB19C0" modified="2012-10-03" version="1">MSECEYCKSTNMLPLSGKYYCEECEIYYIYKDKKQEYSNLPIEDVNQMCVGDHLCGRCKSKYYEKKTIVCTTFREYFRKLPLCKKCKKSNEKSIKNAFFRNFILYKMHGRIFSIPFIALHTIWFYLMGNTVLYKLIVINLIAYKKKELRPYKCLLSSIFITIFGFWNWSDIPFYLYCLFSMVSSKRWYYKVPVNLDGAPPNILEYLEQLNIGRKKSRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUF6</accession>
    <name>I6ZUF6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264808.1">
        <property type="nucleotide sequence ID" value="XM_009266533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUF6"/>
    <dbReference type="GeneID" id="20521619"/>
    <dbReference type="KEGG" id="ero:EROM_070600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070600"/>
    <dbReference type="HOGENOM" id="CLU_148816_0_0_1"/>
    <dbReference type="OrthoDB" id="10012212at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008506">
        <property type="entry name" value="SND2/TMEM208"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05620">
        <property type="entry name" value="TMEM208_SND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="142" mass="16290" checksum="6497A625BF7BD740" modified="2012-10-03" version="1">MRGSKAQMRVFEENRVTRRLLLRSMILYNVCTLAMYLVNKERSISTYLVRSIPEALAIYYLYRISAPTITSEGNTTTLVNPGAALSGKGHVSVAFDLLFISMLVKLLLLYSRKSWLLYLFFVISCCYELLYKPFSALKPKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUH6</accession>
    <name>I6ZUH6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alpha/beta hydrolase fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_070850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264833.1">
        <property type="nucleotide sequence ID" value="XM_009266558.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUH6"/>
    <dbReference type="GeneID" id="20521645"/>
    <dbReference type="KEGG" id="ero:EROM_070850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070850"/>
    <dbReference type="HOGENOM" id="CLU_032487_8_0_1"/>
    <dbReference type="OrthoDB" id="5954035at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047372">
        <property type="term" value="F:monoacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034338">
        <property type="term" value="F:short-chain carboxylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050960">
        <property type="entry name" value="AB_hydrolase_4_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012020">
        <property type="entry name" value="ABHD4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794">
        <property type="entry name" value="ABHYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10794:SF63">
        <property type="entry name" value="ALPHA_BETA HYDROLASE 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005211">
        <property type="entry name" value="Ab_hydro_YheT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="4">
        <location>
            <begin position="114"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="2">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005211-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="369" mass="42453" checksum="2B6A65D449299A04" modified="2012-10-03" version="1">MEEMMQCMLGAVIRSNVIQMILLYSVFYFTMCARRPDVFYSGSKRDSVLKMLSLHKNYYPLFFAPFGTIQTVLQLFRRSIVPPGRISLRVEVPHNGHILLDLVETDRMAGKNALLVHGFNGSGDSTYMRGLAGHLSREGYRVFCFNARGIKSKLDSPVFFHIGWTVDLKATAEFILRNYPGTLEIFGFSMGASWVTKFFGEEKLDPRIVKGGAVCLPFDFFKIGIHFLKNPYTRFFNKLLAINFVKYMNRNKEVFKSAGYDTDIIKKCSSVQQIDMMVTTKIFDIKDVNEYYKNQSCVNYIEKINVPFIIINTYDDPIIPAFSIDKEMCMRNKNILLVIAHKGGHLGFLSNRLDRTWAEDVLIDFVNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUK0</accession>
    <name>I6ZUK0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Golgi nucleoside diphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_071200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264868.1">
        <property type="nucleotide sequence ID" value="XM_009266593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUK0"/>
    <dbReference type="GeneID" id="20521680"/>
    <dbReference type="KEGG" id="ero:EROM_071200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071200"/>
    <dbReference type="HOGENOM" id="CLU_760693_0_0_1"/>
    <dbReference type="OrthoDB" id="6372431at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd24003">
        <property type="entry name" value="ASKHA_NBD_GDA1_CD39_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.150">
        <property type="entry name" value="Exopolyphosphatase. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000407">
        <property type="entry name" value="GDA1_CD39_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11782">
        <property type="entry name" value="ADENOSINE/GUANOSINE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01150">
        <property type="entry name" value="GDA1_CD39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012565143" evidence="5">
        <location>
            <begin position="16"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="171"/>
            <end position="175"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR600407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="326" mass="35992" checksum="46A47DB6310A5E33" modified="2012-10-03" version="1">MDTAALLFLAGIASAFPSSLDPNTPSSIKMTYSGVIDAGTTGTRLNIYGFLDGEIRHQGLFTNASGLANAENDEEIRNSIKELFTHAKPFYSNIKKIPIGFYGTAGFRTLDKPRSDHILEIIKNELKDYNLKEAEVISGSDEGKLALMALVLSNKGADSGHKSMGIIDMGGGSTQISFLEKNGKIVSESIDLGITALGLSEPEECKARENNEQELCARGIMSKLVNMAAMPSLNNIDELYLLSYFHDEFGKVIRNEKTTIREIKDKFYGKCSLLESNECRRLFYLISFIRNLGIDDSKPIRQIDTNNGINVSWASGKGYELNKKYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUV2</accession>
    <name>I6ZUV2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265008.1">
        <property type="nucleotide sequence ID" value="XM_009266733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUV2"/>
    <dbReference type="GeneID" id="20521830"/>
    <dbReference type="KEGG" id="ero:EROM_080950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080950"/>
    <dbReference type="HOGENOM" id="CLU_921423_0_0_1"/>
    <dbReference type="OrthoDB" id="2192067at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="312" mass="36777" checksum="4AC1030C15733F36" modified="2012-10-03" version="1">MDLFRKDDLLGKGEKSSFLYLILRLIVIGIGFYRKSFIGKKINIIRPMCLSLSLTNIYMFIYLDNAISRKNLEQCKAYWVIIVMASLEASVVVETIFKFGDNDMEVIFFSLVYLGLCLCEFIRVHFICKEFGDMFEWIYFKHGGANTNMKIAYKTRARYEAARSLMILAAIQGIYLQIEGIKHCNGDNSMGAIYIIIDHLLEVITFFLMFVRQNEENEEQRMLLIMIVLLNILYSVHLVDKYRSDKKLCQDLKRFFIIRNNFEAAINIIFLYYSILDYRYFGYGLKEMFFPANRRREPLRVEITEGSQKIQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV86</accession>
    <name>I6ZV86_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Leucine-rich repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090180</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265133.1">
        <property type="nucleotide sequence ID" value="XM_009266858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV86"/>
    <dbReference type="GeneID" id="20521957"/>
    <dbReference type="KEGG" id="ero:EROM_090180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090180"/>
    <dbReference type="HOGENOM" id="CLU_000288_18_23_1"/>
    <dbReference type="OrthoDB" id="660555at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055414">
        <property type="entry name" value="LRR_R13L4/SHOC2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF54">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23598">
        <property type="entry name" value="LRR_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00364">
        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="3">
        <location>
            <begin position="111"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF23598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="399" mass="45616" checksum="3A2210136F2DA764" modified="2012-10-03" version="1">MTEVTSADYSKQGYHEIPKMAYTTPINWLILSDNKIREVPESIKELKTLSRLALNDNRIEEIHPGIGSLVGLTWIDLTRNRLRALPDEMENLKRVSGLGLSENNFSEIPKCIFKMTNLRKFGFFSNKIRAIPKDIKFLINLIKIDLSNNEISSLPDEFCELRNLNWLNLSNNKLQKLPDGINNLVRLEELGLGANNLTELPDMSNLKKLRILPVFKNQLTQVNQSIACLNSIEKLDFSDNNITEFPGHVIGIQTLRYLNLKSNKISKIDPSTFKNRVSSVSIIDVSDNLLSCIPLKFFKTFSNVTVIRVGENPFVYHESRPAPSPGLTELCYNKCLNMQYKCEGWIRKRYPDVKNCDACNRLFTTQPHLGYSLSSVGDGSTFVIEKMLCSYDCYRNEYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVD3</accession>
    <name>I6ZVD3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">Histone acetyltransferase</fullName>
            <ecNumber evidence="2 8">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_090690</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="2 8"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265183.1">
        <property type="nucleotide sequence ID" value="XM_009266908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVD3"/>
    <dbReference type="GeneID" id="20564293"/>
    <dbReference type="KEGG" id="ero:EROM_090690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090690"/>
    <dbReference type="HOGENOM" id="CLU_011815_0_4_1"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="9">
        <location>
            <begin position="29"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="292" mass="33388" checksum="1E8EA4E7DDC715CB" modified="2012-10-03" version="1">MNLDNEQIDMLKDDPEFKEYYETMERKNGTTEAIPSMKFLRRNVEVTQIMLGDELLSLPNRIWLFSDKNVVYICDQCLLQYWDSVSFDNHRKDCSSGAPGKYVYIDEKEGISILEIDGKNESVLCRRICTIGKGFIRRKTLYLDVDGYLFYVLFCGGKLGGFFSKEKESTEHNLSCLLVLPPYRSKGYGSLMIDLSYILGTGTPEKPLSKEGRTVYKKYWRNKVLNTLKKATEQEMSIKDISAESGLSIDDTIHGLELLGISPESHIYDVTDKVSVLSKECKKDCLVLQVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVJ2</accession>
    <name>I6ZVJ2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CMP/dCMP-type deaminase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265253.1">
        <property type="nucleotide sequence ID" value="XM_009266978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVJ2"/>
    <dbReference type="GeneID" id="20564364"/>
    <dbReference type="KEGG" id="ero:EROM_091400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091400"/>
    <dbReference type="HOGENOM" id="CLU_085162_0_0_1"/>
    <dbReference type="OrthoDB" id="3180714at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="262" mass="30047" checksum="0DE9FE2420E4243D" modified="2012-10-03" version="1">MFTRLKTVEEEREVCILKGYAVKTSRLDTKRFLRLMEYRRLPKHIKRIKPWNGDLLVLVDIVEDGDPINDVVNLVARMVERGFGSELSFDDPVSLIETVSVPKYQPLTDAQYIESTALWPCIRSSRTTEELNIEYIKTMIEEVKTRKNAPICCGTCIIADGKRALSIHEDTDNILGHSILKGIEEISKAQISYLCTGLDAFIFREPCLSCSMALVHGRIKRVFCINRVSKGAFSSLKINYNRSLNHRYPVYFMNEDYGDKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVX7</accession>
    <name>I6ZVX7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcription initiation factor TFIID subunit TAF6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_101010</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265413.1">
        <property type="nucleotide sequence ID" value="XM_009267138.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVX7"/>
    <dbReference type="GeneID" id="20564531"/>
    <dbReference type="KEGG" id="ero:EROM_101010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101010"/>
    <dbReference type="HOGENOM" id="CLU_021711_3_1_1"/>
    <dbReference type="OrthoDB" id="361039at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046695">
        <property type="term" value="C:SLIK (SAGA-like) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22931">
        <property type="entry name" value="HFD_TAF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08050">
        <property type="entry name" value="TAF6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.770">
        <property type="entry name" value="TAF6, C-terminal HEAT repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037796">
        <property type="entry name" value="TAF6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011442">
        <property type="entry name" value="TAF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046344">
        <property type="entry name" value="TAF6_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10221:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02969">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07571">
        <property type="entry name" value="TAF6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="356" mass="40993" checksum="CE207894F5846CD8" modified="2012-10-03" version="1">MLFSKETLKSFAQSKGISNIDDDALRVLSQDLEYRIKEVCQEGSKFMVGSKRTKLSIDDINYALISRNVDPLFGYDPQESLVFRGLPSNVYYVPDEEIDLEEYLDRPLPKIPLRPSIQSHWLAIEGVQPQIPYNPILLEKPVAKKDTLGTYQEEAELKSQNRHMLTKELGMYFDKVIQAMETDEQIAMECLHNESGIQQLVPYFVHHFNEQIMKNIKNKERLMTVIMVYNSLLRNKYIFIDPYLHQVLPSLITCVIGKSVDDEVRRVAADVVKYVFSNFSGSYKTLAPRIINTLSKAWLDKEKTESTQYGALLCLSSLSKHVVETVIKPKTDYYVKEINNPKVTELLKEVLKADEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVY2</accession>
    <name>I6ZVY2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA excision repair protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_101060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265418.1">
        <property type="nucleotide sequence ID" value="XM_009267143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVY2"/>
    <dbReference type="GeneID" id="20564536"/>
    <dbReference type="KEGG" id="ero:EROM_101060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101060"/>
    <dbReference type="HOGENOM" id="CLU_053731_2_0_1"/>
    <dbReference type="OrthoDB" id="5368863at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000715">
        <property type="term" value="P:nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.530.10">
        <property type="entry name" value="XPA C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000465">
        <property type="entry name" value="XPA/RAD14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022656">
        <property type="entry name" value="XPA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037129">
        <property type="entry name" value="XPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10142:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-A CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05181">
        <property type="entry name" value="XPA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="XPA C-terminal" evidence="4">
        <location>
            <begin position="72"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="23139" checksum="E419C0DE93721A09" modified="2012-10-03" version="1">MPYADCMKNDLQDGGFFAEKEGGEREATEDESILLPISLDNRCKYCLQIPLDSEMKKTFGISVCRTCRCNALKFVTKTSCISDYLLTNDELKELRFLERPNPRKGTWSNMYLYLQSEVEEIAIRKWGSLDKVEETKRGRKRRADDRKIRRLKKRIKELRRRTRIDVRNDEKHIHTFKVEGDICRCECGMSVEQEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZW03</accession>
    <name>I6ZW03_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Inositol polyphosphate-5-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_101260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265438.1">
        <property type="nucleotide sequence ID" value="XM_009267163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZW03"/>
    <dbReference type="GeneID" id="20564556"/>
    <dbReference type="KEGG" id="ero:EROM_101260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101260"/>
    <dbReference type="HOGENOM" id="CLU_011711_5_5_1"/>
    <dbReference type="OrthoDB" id="2190565at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF300">
        <property type="entry name" value="TYPE II INOSITOL 1,4,5-TRISPHOSPHATE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inositol polyphosphate-related phosphatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="322" mass="36582" checksum="F776CF56EEEDCACA" modified="2012-10-03" version="1">MLVTVLSWNVNNKEGVGDAIPKILGHTNSDMILISLQEHFDVYSSVIKPVLKMYPSYRVISVDRVFGLWSIVLSKERYTASTIRMGFGPLGLINKGVCVTKISNGVIFISCHLSAHQENNKKRLKEIRKIFECICDEESLKSTDTVILAGDMNFRISGGLKNLDYSKAKENDQCNEFKRAYPSFSEGIIDFKPTYKYIVATNELCSERQPSWCDRVFVSTSYTSTFNAYRSAYNVKISDHKPIVCVFEVGGKRIDRTAIPMVGYNGHQVIRGFTRFYCGIYEYRNTAFVCLLVLVLYLMLIRSKTIPGLSSLFRRFGPGAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWE8</accession>
    <name>I6ZWE8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA mismatch repair protein MutL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_111150</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265593.1">
        <property type="nucleotide sequence ID" value="XM_009267318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWE8"/>
    <dbReference type="GeneID" id="20564714"/>
    <dbReference type="KEGG" id="ero:EROM_111150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111150"/>
    <dbReference type="HOGENOM" id="CLU_004131_0_2_1"/>
    <dbReference type="OrthoDB" id="10263226at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032389">
        <property type="term" value="C:MutLalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.100:FF:000001">
        <property type="entry name" value="Mismatch repair endonuclease pms1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="entry name" value="MutL, C-terminal domain, dimerisation subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="entry name" value="MutL, C-terminal domain, regulatory subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MLH, PMS, MUTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073:SF52">
        <property type="entry name" value="MISMATCH REPAIR ENDONUCLEASE PMS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="DNA mismatch repair protein MutL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MutL C-terminal dimerisation" evidence="4">
        <location>
            <begin position="461"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="366"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="633" mass="72033" checksum="8BE808F152149ACC" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWF9</accession>
    <name>I6ZWF9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Shelterin complex subunit TPP1/Est3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265608.1">
        <property type="nucleotide sequence ID" value="XM_009267333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWF9"/>
    <dbReference type="GeneID" id="20564729"/>
    <dbReference type="KEGG" id="ero:EROM_111300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111300"/>
    <dbReference type="HOGENOM" id="CLU_1026829_0_0_1"/>
    <dbReference type="OrthoDB" id="2190819at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019437">
        <property type="entry name" value="TPP1/Est3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10341">
        <property type="entry name" value="TPP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Shelterin complex subunit TPP1/Est3" evidence="7">
        <location>
            <begin position="29"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="194"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="263" mass="30523" checksum="BF2DC77DB773162E" modified="2012-10-03" version="1">MLLFLRDFWIEKTIYEQMYDGHWRRRQQASFNAQVIDIGKKVKDGGFEVCVSDSRHFIDSILTKESIEAFGKEIACGIEDIRGCYITVDKFHYVYHFESKRFLMCIERFTYCGGECDTYGEPSDINEMCFLKSLIDSPLYMRFKPEELVMHRSAIEGMNDGERSYLMTILEQNNDLCGNECRLCKGYKGDGEKPGDLGPREGKIKRYNPFSDRPLDGNDTKADGEKIYIFDGIYIEKEGQGELRSKWLDLCSDSSEDEHNNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWM0</accession>
    <name>I6ZWM0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_111850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265663.1">
        <property type="nucleotide sequence ID" value="XM_009267388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWM0"/>
    <dbReference type="GeneID" id="20564784"/>
    <dbReference type="KEGG" id="ero:EROM_111850"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111850"/>
    <dbReference type="HOGENOM" id="CLU_073111_0_0_1"/>
    <dbReference type="OrthoDB" id="1093at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035298">
        <property type="entry name" value="PSMD13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10539:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="330" mass="38436" checksum="214C87A7B9E55BE6" modified="2012-10-03" version="1">MEFDKIKKLQGERKWMQFCCMIIDSMNTHPIDKLLDVFLESAGQIHPRSSYEVFLAFSERLAPEEGIRVLAIGEDIIGGSVLYSELFEVEKMILEIKKCLLLIEINELKGIEKRLFEWKNLKMPRNVRIMYNLLGFKFYQKIQNVEAAFSHLLKYVNLSETEELMDTLVEYALLSKEFFNFTLIASLPGFDKMKNKNLKEIFMMFREGNVKGLEEKHNKLNEIFGSSADIIKEKAYMIALINICFSEVGKKVSIKKIEELLKIPSKMVIYIVLKSFGLGLINGWIDGEEGILYFDSLIPRALQKEEVVKLKEKYDIWEKKVQEVIVMLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACR0</accession>
    <name>I7ACR0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Forkhead/HNF3 transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_010250</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263867.1">
        <property type="nucleotide sequence ID" value="XM_009265592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACR0"/>
    <dbReference type="GeneID" id="20520652"/>
    <dbReference type="KEGG" id="ero:EROM_010250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010250"/>
    <dbReference type="HOGENOM" id="CLU_103809_0_0_1"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000135">
        <property type="entry name" value="forkhead box protein G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881">
        <property type="entry name" value="CHECKPOINT SUPPRESSOR 1-LIKE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45881:SF1">
        <property type="entry name" value="FORK HEAD PROTEIN HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="8">
        <location>
            <begin position="41"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="6">
        <location>
            <begin position="41"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="216" mass="24785" checksum="EE4E6A190EED5CE6" modified="2012-10-03" version="1">MFGKEKCGDEDGLPRCSANERKGFSHSSDPDSHDQGKKGRRPGISYADLITEAIESSSEGMLTLKEIYSYISSKHPYFSLKKTGWQNSIRHNLSLNKSFYKVPRTSVNPGKGSFWKINYEFQNNKGSQKTYRSRNKYSFNSHRDAEKNVNSISELLGPQQGFFDNIGVTEVPFERHTTIFDGNLTSLSDCLEHNYQKEYFDHNDDVSNANYIFSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACW8</accession>
    <name>I7ACW8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010910</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263932.1">
        <property type="nucleotide sequence ID" value="XM_009265657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACW8"/>
    <dbReference type="GeneID" id="20520718"/>
    <dbReference type="KEGG" id="ero:EROM_010910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010910"/>
    <dbReference type="HOGENOM" id="CLU_1320880_0_0_1"/>
    <dbReference type="OrthoDB" id="2193465at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="80"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="123"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="208" mass="24349" checksum="CBEACC674DC08F8E" modified="2012-10-03" version="1">MDILSEDGEGVFDERVRVRKRNVLSNITNKSGDISMAYKDGNGKDVKIDREKDIEDQDGFLSGTGPGNVIRGKVVFKDFTSEDGRNQDTGERKAESKENGSDSVVSRIQKEFEELKEMHRKALGARRKLIKTMEKEMSELKQRMEAKQKVEMENLKRQYDEKLREKQEAYKRICKEKILEYKRKLDEAYRVKVMELKERYGALRKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADM4</accession>
    <name>I7ADM4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rho/Rac/Cdc42-like GTPases guanine nucleotide exchange factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_030440</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264162.1">
        <property type="nucleotide sequence ID" value="XM_009265887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADM4"/>
    <dbReference type="GeneID" id="20520955"/>
    <dbReference type="KEGG" id="ero:EROM_030440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030440"/>
    <dbReference type="HOGENOM" id="CLU_018585_0_0_1"/>
    <dbReference type="OrthoDB" id="2272012at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052233">
        <property type="entry name" value="Rho-type_GEFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572">
        <property type="entry name" value="RHO1 GDP-GTP EXCHANGE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46572:SF1">
        <property type="entry name" value="RHO1 GUANINE NUCLEOTIDE EXCHANGE FACTOR TUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="6"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="4">
        <location>
            <begin position="492"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="188"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="795" mass="93262" checksum="22C77EAFEDBCAD60" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE02</accession>
    <name>I7AE02_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Eukaryotic translation initiation factor 6</fullName>
            <shortName evidence="3">eIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">TIF6</name>
        <name evidence="4" type="ordered locus">EROM_040720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer. Associates with the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <text evidence="3">Shuttles between cytoplasm and nucleus/nucleolus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eIF-6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264337.1">
        <property type="nucleotide sequence ID" value="XM_009266062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE02"/>
    <dbReference type="GeneID" id="20521136"/>
    <dbReference type="KEGG" id="ero:EROM_040720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040720"/>
    <dbReference type="HOGENOM" id="CLU_071894_0_0_1"/>
    <dbReference type="OrthoDB" id="4155914at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00527">
        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.75.10.10">
        <property type="entry name" value="L-arginine/glycine Amidinotransferase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00032">
        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002769">
        <property type="entry name" value="eIF6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00323">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10784">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01912">
        <property type="entry name" value="eIF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006413">
        <property type="entry name" value="IF-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00654">
        <property type="entry name" value="eIF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55909">
        <property type="entry name" value="Pentein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="248" mass="27394" checksum="E41178EB2BEC8974" modified="2012-10-03" version="1">MKNSHRPMSYRIDFEGSSEIGAYMTLTNTYCVVGRSQSNNVLKFLQENVSIPIVETTINSIRSVGSQCRGNRHGLLVPHTITDQEIMHIRNTLPENVIVRRIEERLNALGNVILCNDHVAIIHADLDEESENLIKDVLQVPVYRHNIGHEPLVGTFGALNNQGMLVHPLTNQECQKELSELLEVNVIAGTVNGGSPCIGGGVVTNDWMCVAGAKTTNVEMTVIESVFDLSGDQDLEARRRAIVDTIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE16</accession>
    <name>I7AE16_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_040930</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2 3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264357.1">
        <property type="nucleotide sequence ID" value="XM_009266082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE16"/>
    <dbReference type="GeneID" id="20521157"/>
    <dbReference type="KEGG" id="ero:EROM_040930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040930"/>
    <dbReference type="HOGENOM" id="CLU_166290_0_0_1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051775">
        <property type="entry name" value="Homeobox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323">
        <property type="entry name" value="CEH-10 HOMEODOMAIN-CONTAINING HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323:SF7">
        <property type="entry name" value="HOMEOBOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="4">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="2">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="119" mass="14592" checksum="3691C9B75FBAF0C4" modified="2012-10-03" version="1">MNKKIVEYKLVDSEKDIARSYEPRRKPKRSRILLHEWQSMMLEHSFRINPYPDRIEKYNLFLKTKVPIKNIKIWFQNRRAREKSSYEEMEGMASEKEVGYRGLDVNALAFTEEFQYYKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE54</accession>
    <name>I7AE54_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RING zinc finger-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Unikaryonidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE54"/>
    <dbReference type="GeneID" id="20521209"/>
    <dbReference type="KEGG" id="ero:EROM_041460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041460"/>
    <dbReference type="HOGENOM" id="CLU_826470_0_0_1"/>
    <dbReference type="OrthoDB" id="1933281at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF3">
        <property type="entry name" value="GH10162P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="336" mass="38477" checksum="2E0332B9436CC87A" modified="2012-10-03" version="1">MINDSHDIDYQHLKDETVRMINEIQTALPLDGGGEPNTLSNAEASLIGLWKNILLEDNKPVESDIEIGGQIYPNSAQTVTCLIAYYLLENNCADVVEKLICEMKDGKEEVIKIRDDHARFKEIVFEISEDSTRLLENFLESYPSKMLELYLVSHEFLLLIHNGRYDEALKLCLKKLKSFVPTYIRDVKPLLKFLVNPVNVEEALEKNRERLIENFKSEYLEVTGIPNRCYLEELFETGTSAFLQLSSSGNLFFDKDDQTLPVEIKLEKGRNYHSLFICPVLKTLCVGENIPVMLECGHVISLEAANVLSQEGVLNSFKCPYCPEMSRYENILRLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE81</accession>
    <name>I7AE81_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264442.1">
        <property type="nucleotide sequence ID" value="XM_009266167.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE81"/>
    <dbReference type="GeneID" id="20521244"/>
    <dbReference type="KEGG" id="ero:EROM_050120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050120"/>
    <dbReference type="HOGENOM" id="CLU_1137994_0_0_1"/>
    <dbReference type="OrthoDB" id="2191482at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="244" mass="28125" checksum="71C9689321BB4521" modified="2012-10-03" version="1">MKSRKFIIINASLLVVGLTIWYVLHNFYWVFMSDKDADLIFDKSKLRGKFEDNDKVIEGPAFFNFVYKFLSTLDEYVIGDSCVEGVYDKNNVSGKEAYLRDGLEMATKTLFKNKNVKKDLEEILGSNISDIVAAMKKEEKAREAEDVAEGLKDNSHIVTMVSKAFIYILLKERINLSEYNIYLDKVRSNFNEITLEHLKRIGTYEKISLFQGESDECTEIYDYLRIVMFSTSKLIPEDSASTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AED6</accession>
    <name>I7AED6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small nuclear ribonucleoprotein G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264522.1">
        <property type="nucleotide sequence ID" value="XM_009266247.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AED6"/>
    <dbReference type="GeneID" id="20521326"/>
    <dbReference type="KEGG" id="ero:EROM_050940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050940"/>
    <dbReference type="HOGENOM" id="CLU_076902_10_2_1"/>
    <dbReference type="OrthoDB" id="2146at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="4"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="69" mass="7385" checksum="CCE33FF28846BBD1" modified="2012-10-03" version="1">MGKSAVPNLASLLHKNVIVRVDGGHVLKGLLMGYDAFMNIVLSKITEPSEVGGSAIIRGEFVEDITFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEH8</accession>
    <name>I7AEH8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutaredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264572.1">
        <property type="nucleotide sequence ID" value="XM_009266297.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEH8"/>
    <dbReference type="GeneID" id="20521377"/>
    <dbReference type="KEGG" id="ero:EROM_051450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051450"/>
    <dbReference type="HOGENOM" id="CLU_026126_12_1_1"/>
    <dbReference type="OrthoDB" id="415696at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="106"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="22685" checksum="468CEBB1896169DA" modified="2012-10-03" version="1">MDDVERSNLFEELGEYEVIVAYEDDDNKLYSTLKDIDIDYVEVDLGVSEKLRMEFMKHFDVAKLPALVIRGTPVLGDPDKGIREYIKEREARALKKIQDVIDPSKVVLFIKGSPEHPKCGFTRILMDVLLREGVTRDKIAYFDVLSDEDVRKGLKKINNWPTFPQVYIGNRFIGGLDVIRKMSEREMLRDELSDII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF09</accession>
    <name>I7AF09_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">60S acidic ribosomal protein P2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_070040</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264752.1">
        <property type="nucleotide sequence ID" value="XM_009266477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF09"/>
    <dbReference type="GeneID" id="20521562"/>
    <dbReference type="KEGG" id="ero:EROM_070040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070040"/>
    <dbReference type="HOGENOM" id="CLU_114656_0_2_1"/>
    <dbReference type="OrthoDB" id="1227494at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002182">
        <property type="term" value="P:cytoplasmic translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044076">
        <property type="entry name" value="Ribosomal_P2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21141:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="93"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="105" mass="11681" checksum="27C1E3C5E120699A" modified="2012-10-03" version="1">MEYVAAYAMFDKVGKELNEKSITELFNAIGAEIEPETMRLFLSKVSNKSMEEIMLKGKELMASLAISSARESAPTQTADASAPSQTTENKEEKEEEEDFDIFAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF64</accession>
    <name>I7AF64_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exocyst complex component Sec3-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264822.1">
        <property type="nucleotide sequence ID" value="XM_009266547.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF64"/>
    <dbReference type="GeneID" id="20521633"/>
    <dbReference type="KEGG" id="ero:EROM_070740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070740"/>
    <dbReference type="HOGENOM" id="CLU_041566_0_0_1"/>
    <dbReference type="OrthoDB" id="27109at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019160">
        <property type="entry name" value="Sec3_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16092">
        <property type="entry name" value="SEC3/SYNTAXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09763">
        <property type="entry name" value="Sec3_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 coiled-coil" evidence="2">
        <location>
            <begin position="21"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="487" mass="56883" checksum="38E9D80CA166CE97" modified="2012-10-03" version="1">MDSPRMEKEIDDLFLNEGGGKVDQDKINMLERCMGEIPKIYPNLEQVRTKCKEIDLSLSLYTIKLESLAKEMKEIERENTKLENEIRYQTSIYEHLKELLINVEIKEDHFIALESESFDSIDGVCRIEKALEVLDSFCVDEYDIRIVREKKERINDALRGFYKRFITYLGGFMVGSESSGELKVHKGLYGAIKRFKKIIQHSKRYRDYYIVICSIYMARSKKLYEREFGFHLKTVLRLLKEGVTQDKVDSIFETLFESYRSIVRCEDRFLKSMEIEGTCAEIFRNTGLLITEFVEDVYDIADAETLNGLGKSWKEIRPDEDVYGSFQNELRNVYEKLESGYLKKKMGRKENGVGKLEEVLRKSSNEEVKRKAVVLGVQRVMEEEDRGDLKRTIGRWRLLNHMKNVCSERISEVEDAERKVESEFEKSCIDTILGNGKVVENVRGVLPLIGGEKSFRDKVIKKMREMISNNVEEKEVEEVDKLLREAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFC0</accession>
    <name>I7AFC0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ser/Thr protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264892.1">
        <property type="nucleotide sequence ID" value="XM_009266617.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFC0"/>
    <dbReference type="GeneID" id="20521707"/>
    <dbReference type="KEGG" id="ero:EROM_071440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071440"/>
    <dbReference type="HOGENOM" id="CLU_2589742_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="80" mass="9150" checksum="BA4A7F4B160A8507" modified="2012-10-03" version="1">MPIEAAKIQTIGIMHILTVNAIKRHRLQNSTSFIKILFRKPAIAEYIPFCPGFTPKSLVCTFRSTAIMHIPIMIDKHKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFV1</accession>
    <name>I7AFV1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265117.1">
        <property type="nucleotide sequence ID" value="XM_009266842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFV1"/>
    <dbReference type="GeneID" id="20521941"/>
    <dbReference type="KEGG" id="ero:EROM_090020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090020"/>
    <dbReference type="HOGENOM" id="CLU_1224762_0_0_1"/>
    <dbReference type="OrthoDB" id="2192673at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="226" mass="25259" checksum="A3D64524655FFBF3" modified="2012-10-03" version="1">MNAGKNDISVFANSLGLASIVIPTAIYLFDRSAGENHVLRFVSAVSSSLFTIFMLSYWIIRNWRMNVSGGYSKESVLYLVFIVLCLTGLFTCIGETFLYGLGNDRLDILKIFATFSSINYIRFKCLGLLPRPVYRSSGIGNLASIAAIILITCAITINAKTNNMFVVLWSFVLSGAVMASISLAEEIALPKKKNIWDVSKNHLGFFDLIRLILFSLAYYWPERSKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG15</accession>
    <name>I7AG15_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090530</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOC2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265167.1">
        <property type="nucleotide sequence ID" value="XM_009266892.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG15"/>
    <dbReference type="GeneID" id="20521992"/>
    <dbReference type="KEGG" id="ero:EROM_090530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090530"/>
    <dbReference type="HOGENOM" id="CLU_1115748_0_0_1"/>
    <dbReference type="OrthoDB" id="10266662at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030690">
        <property type="term" value="C:Noc1p-Noc2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030691">
        <property type="term" value="C:Noc2p-Noc3p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005343">
        <property type="entry name" value="Noc2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687">
        <property type="entry name" value="NUCLEOLAR COMPLEX 2 AND RAD4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12687:SF4">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03715">
        <property type="entry name" value="Noc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="249" mass="29087" checksum="20B6176D8951DA9B" modified="2012-10-03" version="1">MPQADNKMELEESYLSRLYTSQPSTLPEDIKSYVLNPKNADNEILYLEKYVNVKNPDLSKIIFITEVLGKCLRRHSEFRDYMKLLVELMERYNDYPHSIFCLRIIKSISGSKFYTPLSFYILRILRNAISVKNLTASGRSIDYDMLNPDMERCKSEEHQMFVIEEAGSLLLKHMSMFSKNIGFPELASVVINELKKLRTGIYKEVIGKMIFDINEQREYVLEKRNKLKLNGIDGKAISLFESSIERTIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG22</accession>
    <name>I7AG22_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_090630</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265177.1">
        <property type="nucleotide sequence ID" value="XM_009266902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG22"/>
    <dbReference type="GeneID" id="20564287"/>
    <dbReference type="KEGG" id="ero:EROM_090630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090630"/>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_1"/>
    <dbReference type="OrthoDB" id="10258631at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="13">
        <location>
            <begin position="79"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="340" mass="39894" checksum="F249BCBAAE47A0F4" modified="2012-10-03" version="1">MECKFLAGSNFDTPMKLITDGVWSLSKEHLKILKTAEKCMKSHTIIPFEVLKYRSQIRGNFMEHAIDLCKLKFLSYVENGYKLTYSGHDCLAINTLRLRGLEMMGEKIGIGKESDIYLGVYCGKDCVLKFHRLGRSSFRNVRKNREYASERIDWFGLSRASCKREVMYLEKFKDMNVPAVLDYDRHVIVQELLDYLPLYKTRVSDVKTIFHLMIEFIKDLWRRGYVHGDFNEFNVLVKDDIKVIDFPQCIRSNDERAIHHLRRDFECVLTYFKKKYKYEPEDGWVAFTNELGIDVDPYEFHEMSSEDLPAYSTNESGIQEGVKLLESKIENVYISEHKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG46</accession>
    <name>I7AG46_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265212.1">
        <property type="nucleotide sequence ID" value="XM_009266937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG46"/>
    <dbReference type="GeneID" id="20564323"/>
    <dbReference type="KEGG" id="ero:EROM_090990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090990"/>
    <dbReference type="HOGENOM" id="CLU_915554_0_0_1"/>
    <dbReference type="OrthoDB" id="2193238at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="305" mass="36161" checksum="1526181C5D0B04EE" modified="2012-10-03" version="1">MRGFDGSSEAKEDAIHEPENKELQRLKQENMRLTGEIVILRQNMIALEKQNFAMKEQKSREALDGLRKTEKLKKEVDVLRIESRIRENQSRVFKKQKGNAEIDIKWALSKSNCGIGFTLFPFEFNRLKFLKDFFYSDFCQLDSSSVIKEMSKKISRFKEFLDFYILFSCKADVFRKFFCMVLMNPLFPEEKVKLFNTLPLDWILNFNDEEVISLVKEYIDRNYKQMALFLGRVVRERPFLLNILVSREMFTELAKIDSKAEKRLVSEICKKGGLSLIDYTNIHYIPQEDLKALYKDLYFEVYLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG84</accession>
    <name>I7AG84_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycosylphosphatidylinositol transamidasesubunit Gpi8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_091490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265262.1">
        <property type="nucleotide sequence ID" value="XM_009266987.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG84"/>
    <dbReference type="GeneID" id="20564373"/>
    <dbReference type="KEGG" id="ero:EROM_091490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091490"/>
    <dbReference type="HOGENOM" id="CLU_089387_0_0_1"/>
    <dbReference type="OrthoDB" id="192611at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042765">
        <property type="term" value="C:GPI-anchor transamidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003923">
        <property type="term" value="F:GPI-anchor transamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016255">
        <property type="term" value="P:attachment of GPI anchor to protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028361">
        <property type="entry name" value="GPI_transamidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001096">
        <property type="entry name" value="Peptidase_C13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48067:SF1">
        <property type="entry name" value="GPI-ANCHOR TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01650">
        <property type="entry name" value="Peptidase_C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00776">
        <property type="entry name" value="HEMOGLOBNASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5028124364" evidence="5">
        <location>
            <begin position="18"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="268" mass="30476" checksum="F930145349F5F6E5" modified="2012-10-03" version="1">MSLILLYVLGALAKNYAILLNSSRGFHNYRHMANVYIFYNVLKENGFEDDQILIISYENQIQDVRNVDREGVYIDEDRRIPYSEFRPSASALEDLLNAISGNCPKLKDADESSNVFVYLNGHGNETFLKFGNIRFMTKDDLMSRIPKLAGRVGKVLLVLDTCQADALVDRDALPDNVFIVATSKLGQPAISSFSSSLICTNVVDNFPYFFFKRFGEGINRKAKLTDLFGELNKEPLGSELVFDEEKDFRFEDFFVQNSHSELLPFKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGA9</accession>
    <name>I7AGA9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">V-type proton ATPase subunit a</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential component of the vacuolar proton pump (V-ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265292.1">
        <property type="nucleotide sequence ID" value="XM_009267017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGA9"/>
    <dbReference type="GeneID" id="20564404"/>
    <dbReference type="KEGG" id="ero:EROM_091800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091800"/>
    <dbReference type="HOGENOM" id="CLU_005230_2_3_1"/>
    <dbReference type="OrthoDB" id="10264220at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF61">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="141" mass="16118" checksum="FFEEA6D77E24982B" modified="2012-10-03" version="1">MLLCIPWMLLGKPIYMIFKNMVKREEISSLWINQFIHVVEFGLGLISNTSSYLRLWAVSLAHAQLTRVLHEFTIGKEGFITPVVLSSVYILGTLVLLIGMEGLGSCLHAMRLNWVEFHSKFFRGKGYLFEPLGFNIPLDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGE5</accession>
    <name>I7AGE5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diacylglycerol O-acyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_100180</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.20" evidence="4"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265332.1">
        <property type="nucleotide sequence ID" value="XM_009267057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGE5"/>
    <dbReference type="GeneID" id="20564448"/>
    <dbReference type="KEGG" id="ero:EROM_100180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100180"/>
    <dbReference type="HOGENOM" id="CLU_018190_0_2_1"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004144">
        <property type="term" value="F:diacylglycerol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019432">
        <property type="term" value="P:triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF7">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="371"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="400" mass="46463" checksum="CE732BAE6739D1EA" modified="2012-10-03" version="1">MRNNICEPPRNASSHSLSVHTTEKVVGRGEMHGLKNLAVIIAFLSVGRFIIDNNREFGFLMSLPLQTLSIMECSCFLLAVLMSFVRSLLGFYIEVRKLGVPYIYLNLGAFEAFVAYLNLNYINRIYSSGWSMILNVVYTAKLISFHLVVRELRKDKKVNVAKRLGIKHFLYFLVVPTLCFQLDFPMRTKRNIKGILLKSIQLLIGFLLFVLVMDQYAIPSIYRIIDFEDITTVIENIINFSISTIILFLIFFYAVFYCFLDAVANLTLFYDSCFYQEWWNASSAREFWALWNRPVYLWFKRHIYAPLVSRGMSKEKAGLVVFTVSGLIHEYVASISIKKITGWFFITMLLQVPLIHVTESFRKRFPSLGNFFFWGAFCVIGQPSVLLLYYRSLYLKECHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGK6</accession>
    <name>I7AGK6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265402.1">
        <property type="nucleotide sequence ID" value="XM_009267127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGK6"/>
    <dbReference type="GeneID" id="20564519"/>
    <dbReference type="KEGG" id="ero:EROM_100890"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100890"/>
    <dbReference type="HOGENOM" id="CLU_1906719_0_0_1"/>
    <dbReference type="OrthoDB" id="2192274at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="142" mass="16687" checksum="E8F31024C9D2B020" modified="2012-10-03" version="1">MIWDIFLPMVMVGPKIKTLLFMNAMVLILVFHFLPTIIEERRLSKKVEGMKRRFNLMLREGNESEEKLRMLKALINNARHYKYGDIPLEGSVEEVRKAYDELRICNGPVFLRLSSIYNISIFFPVFTPVLIQSIKILKHKTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGN0</accession>
    <name>I7AGN0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin conjugating enzyme E2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_101200</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265432.1">
        <property type="nucleotide sequence ID" value="XM_009267157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGN0"/>
    <dbReference type="GeneID" id="20564550"/>
    <dbReference type="KEGG" id="ero:EROM_101200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101200"/>
    <dbReference type="HOGENOM" id="CLU_030988_10_2_1"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000090">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-17 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="162" mass="18289" checksum="447A635AC8845E79" modified="2012-10-03" version="1">MSTPAKRRLMKDLKRVRKNSFKTIFAEPLEDDLMTWVAVIFGPESTPFEGGTFSLVLAFPETYPQDPPNVRFVSEMFHPNIYPNGELCLDILNNRWSPSYDVMGVLISIQSLLNDPNTASPANTDAATLFLSNPILYAEKVKNSVVKSWVDVENLRDKYKGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGR4</accession>
    <name>I7AGR4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Dynamin-like vacuolar protein sorting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_101600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265472.1">
        <property type="nucleotide sequence ID" value="XM_009267197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGR4"/>
    <dbReference type="GeneID" id="20564590"/>
    <dbReference type="KEGG" id="ero:EROM_101600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101600"/>
    <dbReference type="HOGENOM" id="CLU_008964_5_0_1"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019762">
        <property type="entry name" value="Dynamin_GTPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <end position="293"/>
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    </feature>
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        <location>
            <begin position="545"/>
            <end position="631"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="631" mass="71572" checksum="D407A18114064DB9" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH17</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111190</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH17"/>
    <dbReference type="GeneID" id="20564718"/>
    <dbReference type="KEGG" id="ero:EROM_111190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111190"/>
    <dbReference type="HOGENOM" id="CLU_640976_0_0_1"/>
    <dbReference type="OrthoDB" id="2194610at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="428" mass="46381" checksum="CA7DBF11DA6DD926" modified="2012-10-03" version="1">MKNQRTVFVLGCITTGIVMVLFGIDFMMPKRSSVRSVSLVERGRMSLFGKKTSTLGKFAERKDNDRIDVLREKIDLINKKLDRVIKIVEELKSSNKPSSATIGKEANGSTYRESRYSNLHPERSSLSSMSLINQPYYLGERGQSMNIDTAESSRKTRPFGIGLDAFHPKYSDSFALHVSSSGSHSIPTIDKTGASKETERKLEPEATLSKSGEVVSSGLPEGIAIGGTISGGERIEKLIGEFLSEGFANAIKPSPVKPEEDIKESQDKSRISKESKRPKNDSNGQVGLGGSGRPVSQKPLLTNEKGSAYTKKNTQENKEVPTSSLDKKDGEVITKETTSVPVGSGLQKPSGLETKPLPSSGTKGMSEIGRSTSHSNIRYADGSRSSVLYQPENEISYEDASSSGTEEEGIMPGLRSGIPTPRISVIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH74</accession>
    <name>I7AH74_ENCRO</name>
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        <recommendedName>
            <fullName evidence="2">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH74"/>
    <dbReference type="GeneID" id="20564753"/>
    <dbReference type="KEGG" id="ero:EROM_111540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111540"/>
    <dbReference type="HOGENOM" id="CLU_170489_0_0_1"/>
    <dbReference type="OrthoDB" id="2193673at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00474">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="2">
        <location>
            <begin position="37"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="12977" checksum="4AD3E90E5C2DD5A4" modified="2012-10-03" version="1">MGDEITQEKSEEASTAETTQTSLYGESDIIQEERNIIRVKVQQKKGTKKVTIIENIPGELRESLLSSLKKEMGCGGILLDDKSSIQLQGDKTNMGIVGYLKRYIKDCRVELNGKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH92</accession>
    <name>I7AH92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265652.1">
        <property type="nucleotide sequence ID" value="XM_009267377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH92"/>
    <dbReference type="GeneID" id="20564773"/>
    <dbReference type="KEGG" id="ero:EROM_111740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111740"/>
    <dbReference type="HOGENOM" id="CLU_2250138_0_0_1"/>
    <dbReference type="OrthoDB" id="2191601at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707800" evidence="2">
        <location>
            <begin position="20"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="105" mass="11069" checksum="D909DF07A17DDD30" modified="2012-10-03" version="1">MMGFIINLLTLVVSTMTLGVSLMNNQNKPIDIPHIKGMGGSDVAVPSESGSDSNNMGDGVDQSGSSLVSRSGLNPFKISANHFLKGRHSDLLPMKGPMSGLKNRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM18</accession>
    <name>I7AM18_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_030880</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264206.1">
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="364"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="285"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="549" mass="62470" checksum="5F9C4AF544CF1755" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMA0</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">EROM_040510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Fungi incertae sedis</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82819.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMA0"/>
    <dbReference type="GeneID" id="20521115"/>
    <dbReference type="KEGG" id="ero:EROM_040510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040510"/>
    <dbReference type="HOGENOM" id="CLU_131052_0_0_1"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="175" mass="20361" checksum="EB4482B683BF1216" modified="2012-10-03" version="1">MNRKVYKKNKKVVKLLSKIGYRIPYQVIVECDFVAAMNRYHLGLRHINDLFKSQPKLFITKCVYKKLKEIGRDHKEGISRYLEILHCDHKEVKEPLSCLRRVMRKSNKNHYILASNCTEITDLIGTQRKIPVVSCRGGTLVISADLQEIPMYSGFKTEADEEELNRLEALFSTET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="1">CTLH/CRA C-terminal to LisH motif domain-containing protein</fullName>
        </recommendedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264426.1">
        <property type="nucleotide sequence ID" value="XM_009266151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMJ2"/>
    <dbReference type="GeneID" id="20521228"/>
    <dbReference type="KEGG" id="ero:EROM_041650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041650"/>
    <dbReference type="HOGENOM" id="CLU_1384152_0_0_1"/>
    <dbReference type="OrthoDB" id="2415936at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH/CRA C-terminal to LisH motif" evidence="1">
        <location>
            <begin position="58"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="196" mass="23041" checksum="CAFDAAFB3DFDEB28" modified="2012-10-03" version="1">MAEQGLDRSREDIESLARRIIADHMRFVCADKALILWNRRYRKDNDDPENDKELYSSISTRKRILSLIEKKCTNDAFKICEDLKLFDLGIENEASVKETLSKLVFVDFLRARKHIEAIEFARTFINDENENDKLFTLIGYEDINDARFLEIADSIKREKVVEILNKHLFGKEVGRQLSLLSLALNHYNSILKYQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7AND4</accession>
    <name>I7AND4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gluconate transport-inducing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264766.1">
        <property type="nucleotide sequence ID" value="XM_009266491.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AND4"/>
    <dbReference type="GeneID" id="20521577"/>
    <dbReference type="KEGG" id="ero:EROM_070180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070180"/>
    <dbReference type="HOGENOM" id="CLU_107279_0_0_1"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="240" mass="28242" checksum="84E3B2FCFD0BE61C" modified="2012-10-03" version="1">MNKLFGYIKTYEEACLIVHSVRLGYLNAIQGRLTPEERENIRSGDIFCFVESPNGIRRWTDGKTWSPSKIHGQFLLYQEVPKHMNKSAIGKRNRQKSLLGLNERGRSREKIINWDDKLSLNKKTISILLQNKTYHVIAYFRPMFFRVSLIKIPFFNQMDLALKRYPELLSDEFLATMIGKKDFYSQFSLPLPSNKTIFPEVKRHSLEEIASSVLAEWMRQKMKGNDVGKKYICFYDTNKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANK1</accession>
    <name>I7ANK1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264851.1">
        <property type="nucleotide sequence ID" value="XM_009266576.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANK1"/>
    <dbReference type="GeneID" id="20521663"/>
    <dbReference type="KEGG" id="ero:EROM_071030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071030"/>
    <dbReference type="HOGENOM" id="CLU_1602701_0_0_1"/>
    <dbReference type="OrthoDB" id="2191357at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="170" mass="19150" checksum="3E4DB4E4B3BABBC0" modified="2012-10-03" version="1">MRILFLILLSDACGHMKDPIEIRLSLKPDLRLSVKDVPLANTTIEERGGRYAITIGNSYLSGNGTVNIGKDEFYWDICDRDLGAVICFEGRCLTYTEKGLEMLPCMDPSDFRSASQLFQIEAVARMPKGSNAIYRRMDLVGKVDRLIEPLNSRIADMVDRMDSFDTSSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANS1</accession>
    <name>I7ANS1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080110</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264931.1">
        <property type="nucleotide sequence ID" value="XM_009266656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANS1"/>
    <dbReference type="GeneID" id="20521747"/>
    <dbReference type="KEGG" id="ero:EROM_080110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080110"/>
    <dbReference type="HOGENOM" id="CLU_036209_0_0_1"/>
    <dbReference type="OrthoDB" id="442863at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005655">
        <property type="term" value="C:nucleolar ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039182">
        <property type="entry name" value="Pop1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009723">
        <property type="entry name" value="Pop1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012590">
        <property type="entry name" value="POPLD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731">
        <property type="entry name" value="RIBONUCLEASES P/MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22731:SF3">
        <property type="entry name" value="RIBONUCLEASES P_MRP PROTEIN SUBUNIT POP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06978">
        <property type="entry name" value="POP1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08170">
        <property type="entry name" value="POPLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pop1 N-terminal" evidence="4">
        <location>
            <begin position="55"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="POPLD" evidence="5">
        <location>
            <begin position="239"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="418" mass="49310" checksum="5D1FB9DB66CBE2F1" modified="2012-10-03" version="1">MAEREIIPTNFVEERAKEIQFIETAIESNRKKSMMFQRLPFYLRRRLRSHEKRIKRKKHMRKKDRHGLRTHVWYAKRFEMLKVWNTSIPLRRRMKSSKFIYKSQNSRGFVFDESYKDVVVYSRPDEEVFGIDFDLKSVVQNIRHGSIVFEAIVTEKFLIIITSDLEEMESIASLEEHSRIECCLSLIQVDGLFLDEIDGARRFAKALSHKKKGQIDEAVINECDDAIFVKSLSESESGKILLKRNMVMNLWQDIINAGIVPVCVEELQRLALENDYMVYPFDYPTSRLYRDFERSHVEPIKRKYERTPKSKKMELNINLMYIYTDASVEFVSFELEKGFADRCAFIFNENEDVVGRVIRGGFCFTKGLCRGLCYILCDVEEGDEFYSRNLNSSSFSQIKVTKVFRWPYSAIFKNNGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANT3</accession>
    <name>I7ANT3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosomal protein S15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_080270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264946.1">
        <property type="nucleotide sequence ID" value="XM_009266671.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANT3"/>
    <dbReference type="GeneID" id="20521763"/>
    <dbReference type="KEGG" id="ero:EROM_080270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080270"/>
    <dbReference type="HOGENOM" id="CLU_097347_1_0_1"/>
    <dbReference type="OrthoDB" id="10258210at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="158" mass="17862" checksum="B2E3BCC6790FA01F" modified="2012-10-03" version="1">MSELADIEHVEKEDNESLKKKRTFRRYTYRGVPMEELEKMSFSEVAKLFPSRLRRKVRRGLSSREVQLIKDCIATKAACTFNTEKPEMVRTQARSAIIWPVMIGALVGVHVGNGYLPIEIKPDMVGFKLSEFAPTRVIPKHGRPGIGASSSSKFVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANY6</accession>
    <name>I7ANY6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">cAMP-dependent protein kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265006.1">
        <property type="nucleotide sequence ID" value="XM_009266731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANY6"/>
    <dbReference type="GeneID" id="20521826"/>
    <dbReference type="KEGG" id="ero:EROM_080910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080910"/>
    <dbReference type="HOGENOM" id="CLU_2196939_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="108" mass="12393" checksum="38AB7DA003C28ACF" modified="2012-10-03" version="1">MDRILNKKIILEGLKENKIFPFFKDEDYERILNSLSFVYYKKGSKVWVKDSEFLMSLFEGEIDDGEARRIKAKEVIRKGFTALSAIQGAHIPRECQGYGSHDSGESKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP29</accession>
    <name>I7AP29_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Palmitoyltransferase</fullName>
            <ecNumber evidence="10">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_081430</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="10"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265056.1">
        <property type="nucleotide sequence ID" value="XM_009266781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP29"/>
    <dbReference type="GeneID" id="20521878"/>
    <dbReference type="KEGG" id="ero:EROM_081430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081430"/>
    <dbReference type="HOGENOM" id="CLU_1053846_0_0_1"/>
    <dbReference type="OrthoDB" id="9909019at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006612">
        <property type="term" value="P:protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039859">
        <property type="entry name" value="PFA4/ZDH16/20/ERF2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22883">
        <property type="entry name" value="ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0564">Palmitate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="11">
        <location>
            <begin position="109"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="264" mass="30396" checksum="C812D6C0DAA1D2D5" modified="2012-10-03" version="1">MSSKPRGSFEDQFRIKFVMFIHIKMYTGVLLYRLLFTRSLYGSSLLGLLVVASSLSFLKACLTDPGKVNRKVYHKYIPDMIVFEEGVSTQIRTNRNGDWIRAIRINGTEYQEKYCLECNLFRINGMSHCRECNRCVLDMDHHCIWFGNCIGRNNIRYFHIYLYTLSIVTFINAAFMYGLVIICSRNTLLMLALRILAAAFGALYTLFFCIIFLFTMFNIYVALNSSTSRDFIKNGTGNKKLDIGKACKNLSRLKPSLSFDLPSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7APE2</accession>
    <name>I7APE2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">60S ribosomal export protein NMD3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_090770</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as an adapter for the XPO1/CRM1-mediated export of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NMD3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265191.1">
        <property type="nucleotide sequence ID" value="XM_009266916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APE2"/>
    <dbReference type="GeneID" id="20564301"/>
    <dbReference type="KEGG" id="ero:EROM_090770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090770"/>
    <dbReference type="HOGENOM" id="CLU_027444_4_0_1"/>
    <dbReference type="OrthoDB" id="203821at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048899">
        <property type="entry name" value="NMD_SH3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21193">
        <property type="entry name" value="NMD_SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="60S ribosomal export protein NMD3 SH3" evidence="3">
        <location>
            <begin position="230"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="421" mass="49014" checksum="33FC846E75F9D860" modified="2012-10-03" version="1">MILCCKCGILIEPRLMNMCDRCLVTEVTISTKVKRSLSVERCRGCERYLQPPKSWMEFSWGSKEFLLFLIKKNKTLNGLCIVDSNFIYTEPNSKRIMIGLTIRECGIEQPLEMKYVIRNMQCPDCAKVEAKQFWNSLVQVRHKGRHKRMMIYLEHLVLKNSAYEDTSNIKQRKEGLDFYYVDRNAAIRMVNFLQNVLPVRTKMSERLISKDVHTSKCNYKFSYSVEIAPLCKDDLVVVGEKVKQSLGIGSLAVIQKIATSIVLFDVNAMKSVKIAGSFYWSNHDEFRILMSSKDFTRYTVVMKNSRRREGIGYDLTVTSNQNEFSEARFHLGNVEEDDEMLGYDLRHLNLTVECADKPEVVLVRKMPRNDVVWKIDIANENKMEYRLFVEDVEKDKEMIEGLCTIREEEGIIKEIKTFKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7APL4</accession>
    <name>I7APL4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IkappaB kinase complex protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_091900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265301.1">
        <property type="nucleotide sequence ID" value="XM_009267026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APL4"/>
    <dbReference type="GeneID" id="20564414"/>
    <dbReference type="KEGG" id="ero:EROM_091900"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091900"/>
    <dbReference type="HOGENOM" id="CLU_283142_0_0_1"/>
    <dbReference type="OrthoDB" id="2190800at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056165">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006849">
        <property type="entry name" value="Elp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12747:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23797">
        <property type="entry name" value="Beta-prop_ELP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017233">
        <property type="entry name" value="IKAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ELP1 N-terminal second beta-propeller" evidence="1">
        <location>
            <begin position="281"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1102" mass="127417" checksum="00774E1B04A27A24" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7APM8</accession>
    <name>I7APM8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">26S proteasome regulatory complex protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265321.1">
        <property type="nucleotide sequence ID" value="XM_009267046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APM8"/>
    <dbReference type="GeneID" id="20564435"/>
    <dbReference type="KEGG" id="ero:EROM_100050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100050"/>
    <dbReference type="HOGENOM" id="CLU_031814_1_1_1"/>
    <dbReference type="OrthoDB" id="1452at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019585">
        <property type="entry name" value="Rpn7/CSN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045135">
        <property type="entry name" value="Rpn7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14145">
        <property type="entry name" value="26S PROTESOME SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10602">
        <property type="entry name" value="RPN7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="2">
        <location>
            <begin position="180"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="368" mass="43205" checksum="BBC6CF1D83E75E3F" modified="2012-10-03" version="1">MDFDFKEPTLFVHDLIHETKLGKPEAKKKLEKLIVDKCMYDLYKFLVEKGVLRPDHDRMESLLNCKNKKIEELEELRKEETDNETYRSEVDRRIAELYSQVMDTKNGFEWMRKLIRDDMSLSLKMDIFLCKARMGLILGNKKIVEESIKVADDTYERGCDWDRRNRYKVYKGLFKMMAREFKDAGVLFSEVLPSFESSEVMNYSRAVRYMVFCGLLGFERKEIETRILKCSDVMGSSEKLGVQLATSLFECNYGNFMNDLYLFCKSLQDDVFVGRFVNLFCREMRLRVYGQVLESYRLMLLENMAQTFGVSVEYIEQDLGEFIVEGRLWGKIDRISGVIEVTSREGQDASSIVGYGCDVVRRIKKYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ62</accession>
    <name>I7AQ62_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_011080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263949.1">
        <property type="nucleotide sequence ID" value="XM_009265674.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ62"/>
    <dbReference type="GeneID" id="20520735"/>
    <dbReference type="KEGG" id="ero:EROM_011080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011080"/>
    <dbReference type="HOGENOM" id="CLU_154750_0_0_1"/>
    <dbReference type="OrthoDB" id="186625at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="1">
        <location>
            <begin position="66"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="131" mass="14981" checksum="3426A189417FBEBB" modified="2012-10-03" version="1">MIEKIESDTIYDLITNGGEMTLEALRQFIDIVFLGVNPQQVSQLTQSLGNTVTRDDFNAVLDALVLKKAGCLDIFNSWDKGQKGYIDKSDVETILKSYGLSFKESYVDTMVGIFKDKRMKFDDFNRLYKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ79</accession>
    <name>I7AQ79_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Arginine methyltransferase-interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_110520</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265531.1">
        <property type="nucleotide sequence ID" value="XM_009267256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ79"/>
    <dbReference type="GeneID" id="20564651"/>
    <dbReference type="KEGG" id="ero:EROM_110520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110520"/>
    <dbReference type="HOGENOM" id="CLU_109009_0_0_1"/>
    <dbReference type="OrthoDB" id="3863715at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031499">
        <property type="term" value="C:TRAMP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071031">
        <property type="term" value="P:nuclear mRNA surveillance of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071039">
        <property type="term" value="P:nuclear polyadenylation-dependent CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071036">
        <property type="term" value="P:nuclear polyadenylation-dependent snoRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071037">
        <property type="term" value="P:nuclear polyadenylation-dependent snRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051644">
        <property type="entry name" value="TRAMP_AT-DNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46543:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="8">
        <location>
            <begin position="83"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="221" mass="25609" checksum="29C7110B36051C09" modified="2012-10-03" version="1">MKKPLTLLDYAKDVKGIKTVELEDLDICLLDPHISLEKIDTNTLYNILGLVYHFEDVKTENKTRASTKSTNSKPRYFCDTTTCFRCGEMSHEFRECTKTFKKDICVLCSRNGHVREQCPCRYCTRCRGFGHSLDVCPSPRDLDMQEMCKACPAGKHSIEDCPRVWKRYKLNGNTSRYTIYKACPKCFSRNHFIDDCSSSDRAQSSIFNSSYFELANFYNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQC7</accession>
    <name>I7AQC7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Mps1-like Thr/Tyr dual specificity protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_020420</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264014.1">
        <property type="nucleotide sequence ID" value="XM_009265739.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQC7"/>
    <dbReference type="GeneID" id="20520800"/>
    <dbReference type="KEGG" id="ero:EROM_020420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020420"/>
    <dbReference type="HOGENOM" id="CLU_466934_0_0_1"/>
    <dbReference type="OrthoDB" id="20524at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033316">
        <property type="term" value="P:meiotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14131">
        <property type="entry name" value="PKc_Mps1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000131">
        <property type="entry name" value="Dual specificity protein kinase TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE TTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22974">
        <property type="entry name" value="MIXED LINEAGE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="288"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="177"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="585" mass="67446" checksum="6100CEDB20569D64" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQD9</accession>
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            <fullName evidence="9">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
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        <name evidence="7" type="ordered locus">EROM_111120</name>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQD9"/>
    <dbReference type="GeneID" id="20564711"/>
    <dbReference type="KEGG" id="ero:EROM_111120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111120"/>
    <dbReference type="HOGENOM" id="CLU_091940_0_0_1"/>
    <dbReference type="OrthoDB" id="76676at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="131"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="281" mass="33035" checksum="23AF53EBBCDDA7BA" modified="2012-10-03" version="1">MKSYKLVYEPEHGEKRFLFTNVDVKELSEPVTISRERPLREEVVFNTKKKSEQVEYEDEEYRKLKEKEGCLLILEDAENRVYSGKMQDLGASSSCYFVFINTGTYLKVVPIRKWYRFSQKSQLDIIQEYNMEGVGEENGGGEGDKTSSEEREEIDFDDDFDDDDGEETNVFVVREKRLNSAGRRMRNIMENYEEKEESDISEEMEEKDMKDVGKVEKVLTKDVLRGMFRGGRIALRDLLRGVKTRFGLNDEEKELVKEFIGENCTFDTDERNKEKYLVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQM0</accession>
    <name>I7AQM0_ENCRO</name>
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        <recommendedName>
            <fullName evidence="8">Man1/Src1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_111680</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265646.1">
        <property type="nucleotide sequence ID" value="XM_009267371.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQM0"/>
    <dbReference type="GeneID" id="20564767"/>
    <dbReference type="KEGG" id="ero:EROM_111680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111680"/>
    <dbReference type="HOGENOM" id="CLU_524802_0_0_1"/>
    <dbReference type="OrthoDB" id="5376590at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044780">
        <property type="entry name" value="Heh2/Src1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018996">
        <property type="entry name" value="Man1/Src1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN HEH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808:SF2">
        <property type="entry name" value="LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09402">
        <property type="entry name" value="MSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Man1/Src1-like C-terminal" evidence="8">
        <location>
            <begin position="383"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="222"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF09402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="520" mass="59726" checksum="507E2336C1CAF82A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARE0</accession>
    <name>I7ARE0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysophospholipase NTE1</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Intracellular phospholipase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Lysophospholipase nte1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Neuropathy target esterase homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_050030</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NTE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264434.1">
        <property type="nucleotide sequence ID" value="XM_009266159.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="ero:EROM_050030"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Jelly Rolls"/>
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        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
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        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
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        <property type="entry name" value="NEUROPATHY TARGET ESTERASE SWS"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NTE_Ploop"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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        <location>
            <begin position="19"/>
            <end position="904"/>
        </location>
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        <location>
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            <end position="291"/>
        </location>
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    <feature type="domain" description="Cyclic nucleotide-binding" evidence="16">
        <location>
            <begin position="323"/>
            <end position="378"/>
        </location>
    </feature>
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        <location>
            <begin position="610"/>
            <end position="774"/>
        </location>
    </feature>
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        <location>
            <begin position="614"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="14">
        <location>
            <begin position="641"/>
            <end position="645"/>
        </location>
    </feature>
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        <location>
            <begin position="761"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="643"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="761"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN82937.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="904" mass="102993" checksum="FFBA2F474328ABA0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
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        </submittedName>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </reference>
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            <dbReference type="EMBL" id="AFN82952.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
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</entry>
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        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">EROM_051320</name>
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    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Fungi incertae sedis</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
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    <dbReference type="HOGENOM" id="CLU_071633_0_0_1"/>
    <dbReference type="OrthoDB" id="10255118at2759"/>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="341" mass="39832" checksum="0C7D9806844319E6" modified="2012-10-03" version="1">METFGMIEAMRCKSRFSSGDYVGYKNYLRAQMRRPGQKGEERMLCKLESNLSKFFIFNSVGFLKSNLRILRKNESEFGTMYSNLVKGIMGKGVEVNTLLELRKKLMPCRTFVNQVDALLESPPYNFDVSSLKVRHMWNDIPIGFNSSFEKDQFLEGKAPQGVGYDADISRAILKVENKKMRLISLIKTKPGKIICINKKVEELLRALYGLKTVLNENLIESSHTEKLIKDTEELRMYCFNIMEFMKCLKWDDSIDTFRVPSSFKTVDLQILRMREDLSYIPRKCSRNVITKYLEELLRPKKPIIKVPFIPVLFDIARDYISYPAEDRKMSELFKKLHIQND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARS6</accession>
    <name>I7ARS6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_051520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264579.1">
        <property type="nucleotide sequence ID" value="XM_009266304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARS6"/>
    <dbReference type="GeneID" id="20521384"/>
    <dbReference type="KEGG" id="ero:EROM_051520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051520"/>
    <dbReference type="HOGENOM" id="CLU_2133498_0_0_1"/>
    <dbReference type="OrthoDB" id="2191811at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="113" mass="12827" checksum="F9774F010D4464B0" modified="2012-10-03" version="1">MTMDTISLDKGRYKVIEVLYISKSLLSLKLGVEDTKGMHKALIQGESGYKVVYFIDPIDFGAGSRILLEKKTNSLLLRNIDYVITCRKSYRTNTALIEKLLLRNAENMRGVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASR0</accession>
    <name>I7ASR0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_080630</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN83482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266704.1"/>
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    <dbReference type="KEGG" id="ero:EROM_080630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080630"/>
    <dbReference type="HOGENOM" id="CLU_015718_1_0_1"/>
    <dbReference type="OrthoDB" id="10249382at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
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        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="46"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="434" mass="48686" checksum="82DDD033C082AEBF" modified="2012-10-03" version="1">MREVITLQIGQCGNQMGGEFWKVLCKEHGISNCGTLQDDRDLGDRKDVFFYQADDNVFVPRAILVDLEPRVISQASSFFNQENIFLSNEGGGAGNNWAHGYYMGKTMGNDVVDMIQREAEGCDSLETFFLLHSIAGGTGSGFGSLLLERIREEFPKKIIQTYSIFPNNDESSDVVVQPYNSILALQRLIENSDCIIAMDNSSLGRYTLDSLRIGTPTFDHINLLISTVMAASTSTVRFPGYMYCTHQSINSCLVPLDPLKFIVPSYTPFLCDEMSRIVRKTTCSDIMRRLLLPKTRLASYDQSKAQSVVSMLNIFHGVEDSGEVSRTVMRFLDKGMVNFVPWMPPSFNIALGRRIINNTSPNRISGLSLTNSTGISSILSKISGQFDKLKKQRAFLDIYKRFGVELEMFDQGKEVVQKTLEEYHKAEMSTYPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT92</accession>
    <name>I7AT92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090190</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265134.1">
        <property type="nucleotide sequence ID" value="XM_009266859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT92"/>
    <dbReference type="GeneID" id="20521958"/>
    <dbReference type="KEGG" id="ero:EROM_090190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090190"/>
    <dbReference type="HOGENOM" id="CLU_385882_0_0_1"/>
    <dbReference type="OrthoDB" id="2195646at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="720" mass="83789" checksum="1D52BF76F3FB359C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATD2</accession>
    <name>I7ATD2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Endochitinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_090700</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265184.1">
        <property type="nucleotide sequence ID" value="XM_009266909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATD2"/>
    <dbReference type="GeneID" id="20564294"/>
    <dbReference type="KEGG" id="ero:EROM_090700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090700"/>
    <dbReference type="HOGENOM" id="CLU_032939_0_0_1"/>
    <dbReference type="OrthoDB" id="5985073at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016998">
        <property type="term" value="P:cell wall macromolecule catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00325">
        <property type="entry name" value="chitinase_GH19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000726">
        <property type="entry name" value="Glyco_hydro_19_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595:SF197">
        <property type="entry name" value="CHITINASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22595">
        <property type="entry name" value="CHITINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00182">
        <property type="entry name" value="Glyco_hydro_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="Lysozyme-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707629" evidence="6">
        <location>
            <begin position="19"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 19 catalytic" evidence="7">
        <location>
            <begin position="443"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="62"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="180"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="253"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="272"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="333"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="363"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="586" mass="61057" checksum="60ED12BF8812A3AA" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATD7</accession>
    <name>I7ATD7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Translation initiation factor 5A-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090750</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265189.1">
        <property type="nucleotide sequence ID" value="XM_009266914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATD7"/>
    <dbReference type="GeneID" id="20564299"/>
    <dbReference type="KEGG" id="ero:EROM_090750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090750"/>
    <dbReference type="HOGENOM" id="CLU_1687145_0_0_1"/>
    <dbReference type="OrthoDB" id="2189521at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="149" mass="16241" checksum="E2F837D1C59143BE" modified="2012-10-03" version="1">MEKLRFEETPTSPKDIRPGNYIKVDVNGQLRSAQVIDTSSVKNGKHGAAKTTISSKILCTGSNHKGIYTGNDSIIVCRPEKVQLKLIDISGTSITFSDSNGSFDSIDVAGKMSPEDISKIVQTVEGSSNESFDLSLRVLPDFYKLESIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATH0</accession>
    <name>I7ATH0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">deoxyhypusine synthase</fullName>
            <ecNumber evidence="5">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_091310</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="5"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265244.1">
        <property type="nucleotide sequence ID" value="XM_009266969.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATH0"/>
    <dbReference type="GeneID" id="20564355"/>
    <dbReference type="KEGG" id="ero:EROM_091310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091310"/>
    <dbReference type="HOGENOM" id="CLU_039781_0_0_1"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000010">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="332" mass="37151" checksum="C48A9217E4E283D5" modified="2012-10-03" version="1">MDRPQRVKDLSGKVKDDLSFNMEVKGIDFEREWDFTEMIKSFETTGFQGSNLYKAIQEVERMKDSKIFFGCTSNIISSGLRDIIATLVKRKHIHVLVITGGGIEEDIIKTFKPTFCGDFNLCGKDLRENGLNRIGNLVIPSENYEDLEKWLNQVINDITEGYTEERPRILTPSSFIRILGERINDESSILYWAAKNDIPIYSPAVVDGSMGDILSFHPRRKMVKLDVIEDVYNINCETIFCGDTGAIILGCGIVKHHILNANLFKNGLEYCVLINNAQEFDGSDAGANLDEAVSWGKVKPGANGVKIFGDATILFPLIVGATFMKENKKSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATS2</accession>
    <name>I7ATS2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265379.1">
        <property type="nucleotide sequence ID" value="XM_009267104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATS2"/>
    <dbReference type="GeneID" id="20564496"/>
    <dbReference type="KEGG" id="ero:EROM_100660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100660"/>
    <dbReference type="HOGENOM" id="CLU_1251022_0_0_1"/>
    <dbReference type="OrthoDB" id="2194450at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="260" mass="30470" checksum="0DB9365BEA5AD18F" modified="2012-10-03" version="1">MRGFENNDRRHEGREVSDGLLEHNGGEALNGGREAMGHMASQIPYNEFETFVEDYFPEVDEERFIKATSKTIIDEEWLEDIVEHEKEYTRESIIRGLVGCLIDDGDVLGGCLEDSDEDGDVEFNAEDYLEVEEALRSFSSRNVEDLIRLYRKQVTINNKRKEVLARKLRERLAYQGYWMVMRAIDSDMEGLLTKKKGKKKKKEGGEEKMKEILERRSEFIELFKDISMLEDDYKDYEDLFDPKENVDAHEYGIIPYDYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU88</accession>
    <name>I7AU88_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">40S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265544.1">
        <property type="nucleotide sequence ID" value="XM_009267269.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU88"/>
    <dbReference type="GeneID" id="20564664"/>
    <dbReference type="KEGG" id="ero:EROM_110650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110650"/>
    <dbReference type="HOGENOM" id="CLU_1570636_0_0_1"/>
    <dbReference type="OrthoDB" id="2188349at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="169" mass="19056" checksum="A6C9D7F114ADC445" modified="2012-10-03" version="1">MSTQTEAEVNISEIIRKHVKDISVPSEMFSKIAIQIVICDNGEKVMAVKVPRSILPGVQMNYSNIIKAAKQQFHDYYIIFIRNFEIEAREDGNAVTKSMVEKTWLANACFPFLLTGIRTDVRGINDVVINVLLERRTSFSRTEMDVIGAGLNGLLGKNYVVDVNHHTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUF3</accession>
    <name>I7AUF3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-dependent chaperone ClpB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111310</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265609.1">
        <property type="nucleotide sequence ID" value="XM_009267334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUF3"/>
    <dbReference type="GeneID" id="20564730"/>
    <dbReference type="KEGG" id="ero:EROM_111310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111310"/>
    <dbReference type="HOGENOM" id="CLU_005070_4_1_1"/>
    <dbReference type="OrthoDB" id="47330at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000025">
        <property type="entry name" value="ATP-dependent Clp protease subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="entry name" value="Clp, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF18">
        <property type="entry name" value="HEAT SHOCK PROTEIN 104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="Double Clp-N motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="7">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="405"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="851" mass="95348" checksum="0F3193F57274D41C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHA0</accession>
    <name>I6ZHA0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264070.1">
        <property type="nucleotide sequence ID" value="XM_009265795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHA0"/>
    <dbReference type="GeneID" id="20520859"/>
    <dbReference type="KEGG" id="ero:EROM_020980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020980"/>
    <dbReference type="HOGENOM" id="CLU_049639_0_0_1"/>
    <dbReference type="OrthoDB" id="5571054at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070545">
        <property type="term" value="C:PeBoW complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028598">
        <property type="entry name" value="BOP1/Erb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605:SF0">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17605">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BOP1 BLOCK OF PROLIFERATION 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="493" mass="57652" checksum="C3186DB6B073B259" modified="2012-10-03" version="1">MDRIERFLGRRPTKKCRKVKSGGKEFLVTKKDVKLIRSYRKNVCMDPDVDLYRPLNLSFSRKTMDEGIETRRKTPAPQSRRWPKRKIRNGRRKVIEERNKNEIVDIWEEEGLEELNDYEQDMFVKAGEAYAGPVEHLEYRREDLEGDLRRIYLRLYEPREGKSYKLRDLIKDLPRAETLKPFPEEIGLSFRFEKLGAVGISQDFKKFGVGEENKLCVYEFKGFVKVKHVIFDSEIRKIMFTKGETICILLANNTICIVNDTFAEGFSDGQRSFLRKEFVMWSRKETEEGVKSSSCMCTVIKTDGKINDVEAHCDGRYISVVYGKKVMVYDLKEKKELEVLRVRGTVPLKARFHRSLDTMVISTASSLIVYDLVSRKIAREAKEFSFVLDFFSISDNIVVLVNNLKKIILYDWANNVVKRTMLQEDVGMEVIQHRRFNLMCVAYPMEMIVFYNDVGNDLVVPVKRIPGRYRNILFHPHLPWVYGANGNKVCVFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHK5</accession>
    <name>I6ZHK5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_030320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264150.1">
        <property type="nucleotide sequence ID" value="XM_009265875.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHK5"/>
    <dbReference type="GeneID" id="20520943"/>
    <dbReference type="KEGG" id="ero:EROM_030320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030320"/>
    <dbReference type="HOGENOM" id="CLU_700570_0_0_1"/>
    <dbReference type="OrthoDB" id="544788at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056828">
        <property type="entry name" value="Beta-prop_TEP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25048">
        <property type="entry name" value="Beta-prop_TEP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="90"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="129"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="170"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="283"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="TEP-1 C-terminal beta-propeller" evidence="6">
        <location>
            <begin position="327"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="394" mass="43603" checksum="468938926F011A64" modified="2012-10-03" version="1">MSDLVLVEFEDQEGRKVSDRLQIPTKILRSQLQLLANTTLSLYINGQPVLETLEETLKIQNISSAEEVVKIRMSEEEPATQAAFHCSSSFSGHEGPVLCVKNGDVIVTGGGDSTVRFWDPRTKTQSKVVKRHGHWTLSLDISSDGKYVASGALDGSVNLYSISGEYIRSFPGHKKGVVSLVFHKGNLVTGSRDNSVIMWSLSGEVLVSYAHTMAVTCICSGEDYIASGSKDGRIKVYKNLKFFDELRGHSSGINTIDCNGNYMVSGCDGGDVIIWKGFSLYKRMKHNAEVISVSISPNRLYAASGSFDKTVRLWSMDSGQQLLIFRHVDLVYKVRMMNDLIVSCSKDKTIKMFRVSKKKIVSDLVCDDEVYCFDIKGDGGLVCGTKSSRVYFFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHN0</accession>
    <name>I6ZHN0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Small nuclear ribonucleoprotein Sm D3</fullName>
            <shortName evidence="9">Sm-D3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">snRNP core protein D3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_030720</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the snRNP core protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264190.1">
        <property type="nucleotide sequence ID" value="XM_009265915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHN0"/>
    <dbReference type="GeneID" id="20520983"/>
    <dbReference type="KEGG" id="ero:EROM_030720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030720"/>
    <dbReference type="HOGENOM" id="CLU_099537_1_2_1"/>
    <dbReference type="OrthoDB" id="6425924at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01721">
        <property type="entry name" value="Sm_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.100:FF:000002">
        <property type="entry name" value="Small nuclear ribonucleoprotein Sm D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027141">
        <property type="entry name" value="LSm4/Sm_D1/D3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034099">
        <property type="entry name" value="SmD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23338">
        <property type="entry name" value="SMALL NUCLEAR RIBONUCLEOPROTEIN SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00651">
        <property type="entry name" value="Sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="10">
        <location>
            <begin position="5"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="87" mass="9847" checksum="AB83628E2A3D490E" modified="2012-10-03" version="1">MPITVPLKLLHEAQGFIVTIETSNGDMYRGRMKEIDDYMNVVLDEVTVTSFGSCSPRKEAIIRGSSIRFFVLPPALKFAPFFGRYET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHN4</accession>
    <name>I6ZHN4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_030770</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264195.1">
        <property type="nucleotide sequence ID" value="XM_009265920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHN4"/>
    <dbReference type="GeneID" id="20520988"/>
    <dbReference type="KEGG" id="ero:EROM_030770"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030770"/>
    <dbReference type="HOGENOM" id="CLU_037346_0_0_1"/>
    <dbReference type="OrthoDB" id="5327538at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072354">
        <property type="term" value="F:histone H3T3 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024604">
        <property type="entry name" value="GSG2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24419:SF18">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE HASPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12330">
        <property type="entry name" value="Haspin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01331">
        <property type="entry name" value="DUF3635"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="180"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="442" mass="51082" checksum="A927021DE1FC963E" modified="2012-10-03" version="1">MKRFGRKRESIHNTAYEEVRKAFEDIRRKIADTGRLSSSGLSTSTSPGNASWKKDYSCDLAFSSSGDEEDCGLSSIYNSRFKARSKDEAPGFGDEREDDLVINHDVLSSSETYDESLGFESFLDEEIKRESKMQNITFRRKKGIDHHLHGQDQRTEGSVDSDNGGDEMGVLKRIPFDKMPKNIRKIGEATFSEVFAHGTLVYKIIPLGNTSDETSLPSFLKESTIFKAISEEDGVCKLKDVFLVKGRYPREYLKAWDDYGEEENERPSKYEDSQEYGVIVMEDGGESLESIRFQGIEEADRFIRTVIRTLARLEEKYEFEHRDLHWGNILIKQGHINLIDFSLSRLKNGNTVIFNDLNDRQWLFEGDEEVDVQFKVYRDMRELCSGHWERFTPQSNVLWIRYLVEKTFGKNKFKGKKALISQYMSIIDRSTSAKDIDKKLRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHX8</accession>
    <name>I6ZHX8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_040500</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264315.1">
        <property type="nucleotide sequence ID" value="XM_009266040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHX8"/>
    <dbReference type="GeneID" id="20521114"/>
    <dbReference type="KEGG" id="ero:EROM_040500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040500"/>
    <dbReference type="HOGENOM" id="CLU_036019_0_0_1"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="77"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="157"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="199"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="254"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="315" mass="35084" checksum="8D7B5E15FE12789D" modified="2012-10-03" version="1">MGTLNKHVSLALHAMGIYAFFLVFGVYGEKLTTTTYNGRKYESALFPLVLQSLGGIAVGRTMMSYSKEAEGAPGKRVLCHYACLALLSLISAQLGFISLKYLSYPTLIIAKSCKLLPIALMNFLIYRRTLSHRKCLSLSLISISVLSFSLFDKRSTSTSGFSFIGILILITSLLADGIINSAQDHLFRTFKISSFHMMYYTNLFRFLISFTAILLTDNLKYSIAFIKSTPEIVPDLFLYSTFNIFGQIVIYSMVQSHGSLTLTTVNLTRKMLSIFLSLIVFGHRIKKVQALSILGVLGSIALEMTDFKGQKEKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHZ5</accession>
    <name>I6ZHZ5_ENCRO</name>
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        <submittedName>
            <fullName evidence="8">U5 snRNP pre-mRNA splicing factor Prp8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_040700</name>
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    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82838.1"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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        <property type="entry name" value="PRE-MRNA SPLICING FACTOR PRP8"/>
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        <property type="entry name" value="PRE-MRNA-PROCESSING-SPLICING FACTOR 8"/>
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    <dbReference type="Pfam" id="PF08082">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <location>
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            <end position="139"/>
        </location>
    </feature>
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        <location>
            <begin position="229"/>
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        </location>
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        <location>
            <begin position="834"/>
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    </feature>
    <feature type="domain" description="Pre-mRNA-processing-splicing factor 8 U5-snRNA-binding" evidence="5">
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            <begin position="1053"/>
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    <feature type="domain" description="Pre-mRNA-processing-splicing factor 8 U6-snRNA-binding" evidence="4">
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        </location>
    </feature>
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        <location>
            <begin position="1557"/>
            <end position="1714"/>
        </location>
    </feature>
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        <location>
            <begin position="1931"/>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08083"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF10597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF10598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1990" mass="230932" checksum="E5C2678C50F3590E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI02</accession>
    <name>I6ZI02_ENCRO</name>
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        <submittedName>
            <fullName evidence="8">Recoverin-like calcium-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_040750</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the recoverin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264340.1">
        <property type="nucleotide sequence ID" value="XM_009266065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI02"/>
    <dbReference type="GeneID" id="20521139"/>
    <dbReference type="KEGG" id="ero:EROM_040750"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040750"/>
    <dbReference type="HOGENOM" id="CLU_072366_1_0_1"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028846">
        <property type="entry name" value="Recoverin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055">
        <property type="entry name" value="CALCIUM BINDING PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23055:SF178">
        <property type="entry name" value="NEUROCALCIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00450">
        <property type="entry name" value="RECOVERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="7">
        <location>
            <begin position="96"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="184" mass="20946" checksum="7CB321C42C69A64D" modified="2012-10-03" version="1">MGSSSSTIPGSAEEDARQFSHFPPKDIQEWSSSFKSVFPTGYMTSDDLETIFRDFFPFGSPTNFSKRLFETINISQSEAIDFHELLIAYSILIKGSDHEKLRWIFRFYDKDNDGVVSKKEIVEVIQYLADMTSHTLDVSIDSEGIADEIFASVGTVKSFLTFEDFKVLSVRNEKLFKMLVPFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI90</accession>
    <name>I6ZI90_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_050040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_009266160.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20521237"/>
    <dbReference type="KEGG" id="ero:EROM_050040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050040"/>
    <dbReference type="HOGENOM" id="CLU_427009_0_0_1"/>
    <dbReference type="OrthoDB" id="2189586at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038739">
        <property type="entry name" value="ARMC8/Vid28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15651:SF7">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="641" mass="74186" checksum="C2CE03C6B2B4DD24" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIJ3</accession>
    <name>I6ZIJ3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIJ3"/>
    <dbReference type="GeneID" id="20521344"/>
    <dbReference type="KEGG" id="ero:EROM_051120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051120"/>
    <dbReference type="HOGENOM" id="CLU_1586463_0_0_1"/>
    <dbReference type="OrthoDB" id="2190238at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003706814" evidence="1">
        <location>
            <begin position="17"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="168" mass="19312" checksum="8063EAC6B514F152" modified="2012-10-03" version="1">MHSLFVGLVLASKILCFSSEVIYTDWSKSIIRYTTTHPSPHFLAVKYPLNVTPYLISLPVYRIVEERSSTIVIFRLEGKRTLDIPCEKSLCRVWECSPYIDEGVEIKAVGLVGNNVVWEDRVVDWVVIPDFTIQFVAFNSFGILLTIIMKTYCSPENVFRKTRKNSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIM9</accession>
    <name>I6ZIM9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_051480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Unikaryonidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266300.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20521380"/>
    <dbReference type="KEGG" id="ero:EROM_051480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051480"/>
    <dbReference type="HOGENOM" id="CLU_918373_0_0_1"/>
    <dbReference type="OrthoDB" id="2191084at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="301" mass="35617" checksum="773F749090AE3F83" modified="2012-10-03" version="1">MSFIRRRNLISFGTNDSNVDLLDKDAVKYILEKCPSILASIYRRTKQKSKAIQALQYKISGSNKVIYNHEILYNYIYFGDYDGALERAESLSFRDEPIERLLLQIFIHKKTQSEKVCCGSDTYDEISTRVFQYLSTYFTIDILDYFIKALKDFCNNNTECKTKSCGNTKIALFKSQCSKDYLEKLSTESLKYIYHELDIIEALRLIVHRNPKSSERYFREFIERCFPTNEEVKSILGRFYVPSLFELLKERNSLTEDIMKVKEYYTKSCKEVKLESRYCKKAKMIEKNTYLSTCKTFNKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIQ6</accession>
    <name>I6ZIQ6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-tubulin folding cofactor D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264605.1">
        <property type="nucleotide sequence ID" value="XM_009266330.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIQ6"/>
    <dbReference type="GeneID" id="20521410"/>
    <dbReference type="KEGG" id="ero:EROM_060130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060130"/>
    <dbReference type="HOGENOM" id="CLU_345128_0_0_1"/>
    <dbReference type="OrthoDB" id="2191705at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:microtubule cytoskeleton organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR058033">
        <property type="entry name" value="ARM_TBCD_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033162">
        <property type="entry name" value="TBCD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658">
        <property type="entry name" value="BETA-TUBULIN COFACTOR D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12658:SF0">
        <property type="entry name" value="TUBULIN-SPECIFIC CHAPERONE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25767">
        <property type="entry name" value="ARM_TBCD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin-folding cofactor D ARM repeats" evidence="1">
        <location>
            <begin position="158"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="808" mass="93002" checksum="F3835909B2FE0021" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIW7</accession>
    <name>I6ZIW7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">N-terminal acyltransferase complex subunit Ard1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264630.1">
        <property type="nucleotide sequence ID" value="XM_009266355.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIW7"/>
    <dbReference type="GeneID" id="20521436"/>
    <dbReference type="KEGG" id="ero:EROM_060390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060390"/>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_1"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="161" mass="18254" checksum="6C3348454124C0F1" modified="2012-10-03" version="1">MEKEKKAEHVVFRGMRFKDLLQVQQLNVKNSTENFLLGTFLNSLSMSYTTSFVAETDEKVIGYSEAVFFKSKMKGHIYSICVDAPFRRSGIGRRLVNLSIDAMRIESQGRRCEIDLHVRASNVGAISLYRSAGFEIREANIPYYEAGAAGHRMSLWLESST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIY8</accession>
    <name>I6ZIY8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264655.1">
        <property type="nucleotide sequence ID" value="XM_009266380.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIY8"/>
    <dbReference type="GeneID" id="20521461"/>
    <dbReference type="KEGG" id="ero:EROM_060640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060640"/>
    <dbReference type="HOGENOM" id="CLU_657372_0_0_1"/>
    <dbReference type="OrthoDB" id="2193957at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="423" mass="48949" checksum="63A4078BB6B0392B" modified="2012-10-03" version="1">MPLVDFIGSFYKKHDTLYDTFVSACLELKECKDIKNNLTQIYKCLRHEQTRQTSFRCLEYFMDSEMVNFVYKLGHVEEKKVVMDFLVDFTSILPAKCFSRLNGFFDSVFESKDTDELGLALAYSERTIVLGCGIRTALVDYSLRFFFEEGLSGEYSRACIVYLICSKRALDHLKSMRFVQTVIGQTNRMYFDLEKKMPLYLSLLQFISYYARKETDLCKTASDTFDGRKSHFLGMKSSRPHPIRSPLLLARGKDSTEPHSILDLLEFPNVHSAKVYMEILESTGSSNIRALVIDSVLTTIENTDDPTRFIRFCVENHPELVAPYLIKNGGEDWSPTNVLQKVAKMKGKGSSIKVPCVCHVDRHRISIVDFLIENNICDDLIFLFIWAVSKETFYTHFEKIREIFKDHRTIWADLWSLILTPII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIZ2</accession>
    <name>I6ZIZ2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Vesicle coat complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_060700</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264660.1">
        <property type="nucleotide sequence ID" value="XM_009266385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIZ2"/>
    <dbReference type="GeneID" id="20521466"/>
    <dbReference type="KEGG" id="ero:EROM_060700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060700"/>
    <dbReference type="HOGENOM" id="CLU_359811_0_0_1"/>
    <dbReference type="OrthoDB" id="2192743at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="6">
        <location>
            <begin position="28"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="779" mass="88571" checksum="10C18F66D61847F4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJF5</accession>
    <name>I6ZJF5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="4">2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Hexosephosphate aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_071220</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264870.1">
        <property type="nucleotide sequence ID" value="XM_009266595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJF5"/>
    <dbReference type="GeneID" id="20521682"/>
    <dbReference type="KEGG" id="ero:EROM_071220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071220"/>
    <dbReference type="HOGENOM" id="CLU_012520_5_2_1"/>
    <dbReference type="OrthoDB" id="15235at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00528"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00714">
        <property type="entry name" value="GFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10490:FF:000001">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000052">
        <property type="entry name" value="Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047084">
        <property type="entry name" value="GFAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13522">
        <property type="entry name" value="GATase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="11">
        <location>
            <begin position="2"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="376"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="12">
        <location>
            <begin position="548"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="AFN83373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="698" mass="77608" checksum="3E910D53E6FD565A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJI7</accession>
    <name>I6ZJI7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribonuclease-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264905.1">
        <property type="nucleotide sequence ID" value="XM_009266630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJI7"/>
    <dbReference type="GeneID" id="20521721"/>
    <dbReference type="KEGG" id="ero:EROM_071570"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071570"/>
    <dbReference type="HOGENOM" id="CLU_1713231_0_0_1"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="154" mass="17702" checksum="299A4A373376AEA2" modified="2012-10-03" version="1">MFNIEDHYTAHMRKETIRTLSQPFALTNRSVKASKREKQSRGIPKVEREKIKYRDFVEIGKMFIDYLTALKGAMNPSAFVSKMYLCELTGAKIKIVGGRTGIIVEERANSIIVVLEDDSVKTYLKKTTDFTVTHEGIEYVFIGSEMKPNRFIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJL7</accession>
    <name>I6ZJL7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">GTP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_080160</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264935.1">
        <property type="nucleotide sequence ID" value="XM_009266660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJL7"/>
    <dbReference type="GeneID" id="20521752"/>
    <dbReference type="KEGG" id="ero:EROM_080160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080160"/>
    <dbReference type="HOGENOM" id="CLU_047739_0_0_1"/>
    <dbReference type="OrthoDB" id="61815at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF2">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="6">
        <location>
            <begin position="125"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="378" mass="43857" checksum="07A52C2B6BEBDEEB" modified="2012-10-03" version="1">MKIEWGKLIIEKRFGTKQKAKTRVQRASVTEYGGVEKLSEELAMKNKKITGGKPARAMEESEDQSDLDIIVERLIPSVELSKTIPPRVSYERIEKEIFKEVESRIFDLWKGLQGSRVFERNIEIWRQLWITCERSDVIVQIVDARNPEFFLNDDIRKLYPRKEHIILINKADLSSSRVEIKEYRCFYYSALERNSLLKFILEYKGKVIGFVGYPNVGKSSTINSIVNSKRVRVSQTPGKTRHIQTIHIEEGPCLLDCPGLVFPRHDKIDLILHGILNVDQLLDLNGSIDYIVEFIGIGKLCKFYSLKGFYNDSRHSRGVNYMNLMSMSKGWEVGRCLKTIIKDFASGKIPYDRDDEDIPETMFDWYEREIAPQKSCVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJR8</accession>
    <name>I6ZJR8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-(apurinic or apyrimidinic site) lyase</fullName>
            <ecNumber evidence="2">4.2.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_080740</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-1 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264990.1">
        <property type="nucleotide sequence ID" value="XM_009266715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJR8"/>
    <dbReference type="GeneID" id="20521810"/>
    <dbReference type="KEGG" id="ero:EROM_080740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080740"/>
    <dbReference type="HOGENOM" id="CLU_027543_3_2_1"/>
    <dbReference type="OrthoDB" id="238681at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034039">
        <property type="term" value="F:8-oxo-7,8-dihydroguanine DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012904">
        <property type="entry name" value="OGG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052054">
        <property type="entry name" value="Oxidative_DNA_repair_enzyme"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242">
        <property type="entry name" value="8-OXOGUANINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10242:SF2">
        <property type="entry name" value="N-GLYCOSYLASE_DNA LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07934">
        <property type="entry name" value="OGG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="10">
        <location>
            <begin position="117"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="34132" checksum="F111E29442F73452" modified="2012-10-03" version="1">MKDEGWEPLCTEETVDLEKTLHSGQVFSFRQTDEKEYTGVLGTCLVSFLQDGNRVLYKVLNGDKTSKEIELEITSFFTLDVKLCPLLRRWRLDPNNLLVGLRALRYNLIPTIFSFICSSNNNIARITRMVGFLYSKGEFIMKYKNADFYHFPDLEKLVDIEEELKSNGFGYRSSYICNAAKYLLKNRLDHQQASRIREMMVSIKGIGYKIADCILLIGTGNLDVVPVDTHIFKHSKKMFGINEKVLSQKTYGIIQDLYRKRFGEYAGIAQLYIFKGMIDLRKKSKKEKPSSLEACC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKB0</accession>
    <name>I6ZKB0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_090870</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265200.1">
        <property type="nucleotide sequence ID" value="XM_009266925.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKB0"/>
    <dbReference type="GeneID" id="20564311"/>
    <dbReference type="KEGG" id="ero:EROM_090870"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090870"/>
    <dbReference type="HOGENOM" id="CLU_125555_0_0_1"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="8"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="101"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="185" mass="20854" checksum="64292FEDFAA5EBE2" modified="2012-10-03" version="1">MQSTRKTNQLAIPDLPKGVTVKDILEAFPKYGKEITGIKLSKQRRPKNEDLKVGYVEFETIEDTEKAYGQGQVLVAGESRDLHFAKRRDQRMKERVIVSSKKVYISGVSEDTNKDELSSLLGNCKISGGDKGKNYLFAEYDNSEEQEKALNRINTMKINGSKLFAMPAYEKANIGRVGRKRSDSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKD5</accession>
    <name>I6ZKD5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Septin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_091220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265235.1">
        <property type="nucleotide sequence ID" value="XM_009266960.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKD5"/>
    <dbReference type="GeneID" id="20564346"/>
    <dbReference type="KEGG" id="ero:EROM_091220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091220"/>
    <dbReference type="HOGENOM" id="CLU_017718_7_0_1"/>
    <dbReference type="OrthoDB" id="416553at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032156">
        <property type="term" value="C:septin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01850">
        <property type="entry name" value="CDC_Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030379">
        <property type="entry name" value="G_SEPTIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016491">
        <property type="entry name" value="Septin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18884">
        <property type="entry name" value="SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00735">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006698">
        <property type="entry name" value="Septin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51719">
        <property type="entry name" value="G_SEPTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Septin-type G" evidence="3">
        <location>
            <begin position="12"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="273" mass="31072" checksum="C42A2E53C8B80B95" modified="2012-10-03" version="1">MQINKQMIVRRQNRRFTIMAAGPKGSGKSSFFNTLIGKEIVTSKGQEGIDLYMLNLDCEGIMQRITLIDTPGFGETLDDTEIQETICNFIKAQLDMFIAEESKIRRNPKYEDTRVHCLLYFIPSTSFGLKNRDIVFLKKVSGLVNIIPVISKSDGLSITERIEVKRQVVEQMRHYNISIFDLDDPEIYSSPTAGNDLNSLVPFMIVSADGENLESRVRNYQWGSVSIDNPEHCDLPALRELLLSTHIYGLIDYTASEIYENYRAAVLESEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKJ5</accession>
    <name>I6ZKJ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_092040</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265315.1">
        <property type="nucleotide sequence ID" value="XM_009267040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKJ5"/>
    <dbReference type="GeneID" id="20564428"/>
    <dbReference type="KEGG" id="ero:EROM_092040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_092040"/>
    <dbReference type="HOGENOM" id="CLU_1250669_0_0_1"/>
    <dbReference type="OrthoDB" id="2189914at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707318" evidence="2">
        <location>
            <begin position="18"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="221" mass="25262" checksum="0BD6654DF587A75F" modified="2012-10-03" version="1">MSSVFLLIGLWGPCAYSVFNGGDRSFDIFKTSSAGTRTFVAKSMFNTSVVTFSKIYAVLRVPALLYYYYSRSKLMGEKTAIFIESFLLGSEVYLSVALLVILRTKHSFLSEYKSLDSTNLLSFCLMFHGFLTYTINTVAIPFQMTELHYQIEQSLSFGARLILDMLLISMFCPIRDQLFDNFPDKYEKNHEITRVARLEDSGPIVQEIESVIEFEEKEIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKU6</accession>
    <name>I6ZKU6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Rrp4-like RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_101230</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265435.1">
        <property type="nucleotide sequence ID" value="XM_009267160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKU6"/>
    <dbReference type="GeneID" id="20564553"/>
    <dbReference type="KEGG" id="ero:EROM_101230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101230"/>
    <dbReference type="HOGENOM" id="CLU_034114_1_0_1"/>
    <dbReference type="OrthoDB" id="1650at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0271">Exosome</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="4">
        <location>
            <begin position="5"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="RRP4 S1" evidence="5">
        <location>
            <begin position="54"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="225" mass="25422" checksum="C026F543736F1A18" modified="2012-10-03" version="1">MKALFLPGEEVLESDGYIRGHGTQLKDSHIVSTYFGKVRITNKLVAIDPVFPMKYTPEVGNVVIGRVVGIHNKKWKIDLNSRSDVYLGLSAINLPGAVQRRKQESDEMSMRSFFDINDLVVSEIQKVNRNGTAALHTRNDRYGKLSKGLLVFVPHFLLEPLKTRFLFNDSIKVIAGCNGYIWIGRRHEDPAEFVEVAKVVSKVKEMASQNRSINMEKILGKEAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS31</accession>
    <name>I6ZS31_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_010620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263903.1">
        <property type="nucleotide sequence ID" value="XM_009265628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS31"/>
    <dbReference type="GeneID" id="20520689"/>
    <dbReference type="KEGG" id="ero:EROM_010620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010620"/>
    <dbReference type="HOGENOM" id="CLU_801747_0_0_1"/>
    <dbReference type="OrthoDB" id="410307at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 15-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000002">
        <property type="entry name" value="Zinc finger protein 36, C3H1 type-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045877">
        <property type="entry name" value="ZFP36-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547">
        <property type="entry name" value="CCCH ZINC FINGER/TIS11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12547:SF18">
        <property type="entry name" value="PROTEIN TIS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="6">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="5">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="346" mass="40034" checksum="703B76D11AA7A38D" modified="2012-10-03" version="1">MDIFGGKMDKDPWPIETTSIDTNDCTEGYPDKCQCLLAKKASKPRNVCTCFKSNKKLFQGNVIPHISTQSINKEQNPFERRLKGHDSKVLSNSIQTHFSSARGSHTNKKYQLYKTEMCRSHTEIGYCKYGDKCQFAHSKAELRYVQRHPKYKTETCKTFWEEGSCPYGKRCCFIHIPNTDIGNLPVHNHQKDNKRSYRDYELVLGEIDEHSDDRLICSKIDGPVLLVEKDLNQTDEEVGRNAGERFDKSRNVFSGEIHIFNHSFYKSSRQSFEPIIEDEFNGEVMLRRPFWESNYSKIWLDSEASLFYLEGPCRKQSRGGVFAPGGYVYKPFNKKVSDFVLGYLGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS97</accession>
    <name>I6ZS97_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spore wall protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263978.1">
        <property type="nucleotide sequence ID" value="XM_009265703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS97"/>
    <dbReference type="GeneID" id="20520764"/>
    <dbReference type="KEGG" id="ero:EROM_020060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020060"/>
    <dbReference type="HOGENOM" id="CLU_1255984_0_0_1"/>
    <dbReference type="OrthoDB" id="2186354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031513">
        <property type="entry name" value="MICSWaP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17018">
        <property type="entry name" value="MICSWaP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707410" description="Spore wall protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="220" mass="24721" checksum="F43A146A870415DD" modified="2012-10-03" version="1">MFGNKYFLILLMGLEISSQAIGVRRVPVEVYISEAGRKKFLDKGRSIEDIVGSVAEELELKMNEHVYADRASTDKRKFHFDWSVSGMTSPEIDLDKCGKDTSRFQYDLVQAARDKPGASVILLYTCESEGYSEDFILAGQKTPLVIQRKYPECSNDVATFTETEPMKIESIIANALFITAGSPLNDMVEFEEVDNGRDGFKRDIRLKTTDFSLFSGGICH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSB2</accession>
    <name>I6ZSB2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DnaJ-like N-terminal domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_020210</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263993.1">
        <property type="nucleotide sequence ID" value="XM_009265718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSB2"/>
    <dbReference type="GeneID" id="20520779"/>
    <dbReference type="KEGG" id="ero:EROM_020210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020210"/>
    <dbReference type="HOGENOM" id="CLU_829071_0_0_1"/>
    <dbReference type="OrthoDB" id="2190196at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051434">
        <property type="entry name" value="DnaJ_C_subfamily_member5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44027:SF7">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 5 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="335" mass="38462" checksum="044817E04A85DAB2" modified="2012-10-03" version="1">MSLYDVLKIPKDATKENVERSYRFLVRLYCQSKNEMSPDRFAEINKAYTILKDKYKRDFYDIFGEVSVQLLLHNKDSYIITRMFDRLNICFYLVTLVIYISTLLALPSIVAHGKCSSIVMSLPFAVSSVTLTIPMARSLSALYWVYGVGTELKSMIFRSMEINIATLHILNYALYADGFIDILFSGAIFLVVEILSTINTIYYHNDKQKFFLQAKKNIIIGKMIRAALFGLVLVPIIPSFLKPLLFFVQILWGIYSKRYSLKVNMCILLLPILYISTFSLVLAGFRSVLIYLPLWIFGSVVMVVLALVISNVVSNIPRSRYDIREVEALPYYDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSK3</accession>
    <name>I6ZSK3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_021110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264083.1">
        <property type="nucleotide sequence ID" value="XM_009265808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSK3"/>
    <dbReference type="GeneID" id="20520873"/>
    <dbReference type="KEGG" id="ero:EROM_021110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021110"/>
    <dbReference type="HOGENOM" id="CLU_128308_0_0_1"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="6">
        <location>
            <begin position="38"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="40"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="130" mass="14677" checksum="F54BB3CEC8F1DA5F" modified="2012-10-03" version="1">MAAEKTKNVKELEAALGLLKLSGFQMNRYCAGKQMRKTRLQTCVLNRIFEISKFPSSKTIIDLALLINVHPKSIQKWFQNTRQAIRKKGCAKGILVLADPEEHSAMDIPLPVLADIVEVEKRIVSRFGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZT03</accession>
    <name>I6ZT03_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">60S ribosomal protein L3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_031090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264228.1">
        <property type="nucleotide sequence ID" value="XM_009265953.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZT03"/>
    <dbReference type="GeneID" id="20521022"/>
    <dbReference type="KEGG" id="ero:EROM_031090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031090"/>
    <dbReference type="HOGENOM" id="CLU_033361_2_1_1"/>
    <dbReference type="OrthoDB" id="1611972at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="383" mass="43011" checksum="A464C853F9D89334" modified="2012-10-03" version="1">MSCRKFSAPRHGSLQFCPRKRSKTIRPSPGAFPADDSSQPVHLTGFMGYKAGMTHVIRTKIQAAKNKQLSREVMDAVTVLEAPPMVVYGVVGYERTVTGLRRLPIVLAPYVSDGVLRRMFGNKHVERENSKRFCASEETESRISEIKKKAECVRVLVQTQPGLIKKLGLKKAHIAEIQVNGGSISEKVEWCLDMLEKEIHIGEVFKENENIDVIGVTKGKGFQGTVKRFGVRKQPRKSRKGIRKVACIGAWHPSRVMYSVARAGQMGFHRRTEKNKRVYMVGNGNSSIRTEFDLTEKFISPMGGFPHYGEVKNDFIMVKGAVVGPRKRVVTLRKSLDQQRPSEELVIKFVDTSSKIGHGRFQTSDEKRAFYGAKKAEVAPEIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTH8</accession>
    <name>I6ZTH8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide chain release factor subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_050330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264463.1">
        <property type="nucleotide sequence ID" value="XM_009266188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTH8"/>
    <dbReference type="GeneID" id="20521265"/>
    <dbReference type="KEGG" id="ero:EROM_050330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050330"/>
    <dbReference type="HOGENOM" id="CLU_035759_2_1_1"/>
    <dbReference type="OrthoDB" id="10254527at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="386" mass="43257" checksum="F4AB2BC2C5CBB3C1" modified="2012-10-03" version="1">MLDEVEMWRMKKLLRGLRDSRGNGTSMISIIIPPKDQISRVSKMLTDEYGTASNIKSRVNRLSVLGAITSAQSKLKQYSKTPPNGLGIFVGTVLMEQNKEKKVSVGVEPIKPVNTSLYMCDSKFHVDDLLALFEDEVKYGFIVMDGHSTIFATLQGNVKTILQQIHVDLPKKHGRGGQSSVRFARLRVEKRNAYVKKVGEIAGNLFLTNCIMNVEGVILAGQSDLKNELSRVLDSRIKVIKTVDTNYGGEGGLNQAIELCEDILKDVKLSKEKKVLQKFFNEINTESGKFCFTMKETMQCLEMGAVETLIVYENLSDVRDEELFVDWIAENYKNFGCTLVFVSDKSAEGTQFIEGFGGIGGILRYRVDMEDQMDGDYSSVDDDEIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTK5</accession>
    <name>I6ZTK5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_050640</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264493.1">
        <property type="nucleotide sequence ID" value="XM_009266218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTK5"/>
    <dbReference type="GeneID" id="20521296"/>
    <dbReference type="KEGG" id="ero:EROM_050640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050640"/>
    <dbReference type="HOGENOM" id="CLU_026870_0_0_1"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13983">
        <property type="entry name" value="STKc_WNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050588">
        <property type="entry name" value="WNK_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13902">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="53"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="468"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="691" mass="78131" checksum="4658FF463CFAAAF0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTP6</accession>
    <name>I6ZTP6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_051100</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264538.1">
        <property type="nucleotide sequence ID" value="XM_009266263.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTP6"/>
    <dbReference type="GeneID" id="20521342"/>
    <dbReference type="KEGG" id="ero:EROM_051100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051100"/>
    <dbReference type="HOGENOM" id="CLU_018693_3_1_1"/>
    <dbReference type="OrthoDB" id="205248at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05145">
        <property type="entry name" value="RIO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="13">
        <location>
            <begin position="12"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="339"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN83041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="386" mass="44258" checksum="E1F9D065BCFD61D4" modified="2012-10-03" version="1">MKETRGEKRKKDKSDRATVDKVLDKKTLRVLEKLEARGKLKGLEGSLCTGKESNVYLAEASTALCSKFIKSRYSENGVPDSSEQMIPVVVKIFKTSIMSFKDRERYIRSEKRFQRFCTSNPRKLIKVWAEKEVRNLKRLNNAEIPSPEPIYLKNNILIMSRIGNCEEVAPKLRDAMIEDVNGCYLQCVEIIRNMYKKAGLVHADLSEFNLLYFGGVVYVIDVGQSVEIDHANAQSFLIMDINNINNFFSKKGVDVIRGNDLFEEITGSVIPSYLKDMDIVKDVFIPSRVSEIGNLEDLAAFGVERQGSEMASMSEEFSDTNESDIEGFGTRFEAMEIENTEDAKEEKKKKKKTVKEMNRIRRASRISKKEKKKIFKKYIGMKKRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTS0</accession>
    <name>I6ZTS0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">26S proteasome alpha-type subunit C9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_051410</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264568.1">
        <property type="nucleotide sequence ID" value="XM_009266293.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTS0"/>
    <dbReference type="GeneID" id="20521372"/>
    <dbReference type="KEGG" id="ero:EROM_051410"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051410"/>
    <dbReference type="HOGENOM" id="CLU_035750_4_3_1"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="232" mass="25956" checksum="21113C99E2386B08" modified="2012-10-03" version="1">MEDNKLKSNTFTDEGRLPQVEFAIKNVSKAGTIIGYVCVDGIVLIGINKEPTNGPMEKIYQLSDNIYCALCGLFGDAMRIKRYARVKAQEALEKFGVECPLTTLCKAIGQKKQAFTQYAGTRPFGASFLYAGVMGGEYALFSTDPSGTLNRWRGMCYGENEEAINGGLKNDMPEEEMEMKMATIEILKLLGKIRELGPKEADRMEILHFSKESKVFLPRDQILEFLEEVHAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTW3</accession>
    <name>I6ZTW3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polar tube protein Ptp1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264608.1">
        <property type="nucleotide sequence ID" value="XM_009266333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTW3"/>
    <dbReference type="GeneID" id="20521413"/>
    <dbReference type="KEGG" id="ero:EROM_060160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060160"/>
    <dbReference type="HOGENOM" id="CLU_665687_0_0_1"/>
    <dbReference type="OrthoDB" id="2194938at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="380" mass="38470" checksum="1DDAA18C91D2D099" modified="2012-10-03" version="1">MKGISKILSASVALMKLKNVYSKASFCSPTYDSVQPQQGMVQPSYVLIPGTPEAIKNCAYNPQNLYAPFSPTPSSAPFSTPTGTSNENETSPTEDVGTCKISVLKHCDTPGTSATPCESPEQVIQPQPSLTVASITSTIIAPVQASPVVAKVLQPTEMVVTTPSSVVSANQVIPGYYSNGTPAAPGQQGQILSGSVLAPGANLCQVVPGSTPGQVLPGAVPGFPQCQTGQNGSGNGGSSPVPGFPQCQAGQNGSGNGGSSPVPGFPQCQAGQNGSVTGQSGQFSPTPQVPNPVAMPPISGISGNGYPTSTTYASSLGPLGPCVDVQKPSSSCDQQTNERYSMEACAAPTPTVIIGNSEYFVAPGMYNAISSPCNSSCCQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTX3</accession>
    <name>I6ZTX3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcription initiation factor TFIIE subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_060260</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264618.1">
        <property type="nucleotide sequence ID" value="XM_009266343.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTX3"/>
    <dbReference type="GeneID" id="20521423"/>
    <dbReference type="KEGG" id="ero:EROM_060260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060260"/>
    <dbReference type="HOGENOM" id="CLU_056580_3_0_1"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003166">
        <property type="entry name" value="TFIIE_bsu_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02186">
        <property type="entry name" value="TFIIE_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51351">
        <property type="entry name" value="TFIIE_BETA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIE beta" evidence="7">
        <location>
            <begin position="57"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="270" mass="31340" checksum="44B4F5687BF7037F" modified="2012-10-03" version="1">MNSCFTASPFTTRSLVTRSMLAILCSSHDILRWVFFRTIQQLELNTAGSIPDMGHDRIDSIDRGVPRHTNTYIHAVISLLKQHDRPVTFEEIKDKTRIDLHNNYMLLQSIKKNPRIVATHSTLMFKPLYSIRGVEDLRKVMKEINGEEGLEMSKLMDSPVNITPFVEELKRNDEIIVLSDIDGSEIVFWNDMHEKPIDPQIKSLWSQVRISTYHDLIRELNTAGLKTEKVENIKKRPTIKIKKDRKSRRRIRITNTHVKGLDLSGVQDER</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU81</accession>
    <name>I6ZU81_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tetratricopeptide repeat protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_061190</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264708.1">
        <property type="nucleotide sequence ID" value="XM_009266433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU81"/>
    <dbReference type="GeneID" id="20521515"/>
    <dbReference type="KEGG" id="ero:EROM_061190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061190"/>
    <dbReference type="HOGENOM" id="CLU_404909_0_0_1"/>
    <dbReference type="OrthoDB" id="2190636at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="653"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="679" mass="78711" checksum="3E0B5AEFA939C962" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUD0</accession>
    <name>I6ZUD0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nop2-like nucleolar protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_070250</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264773.1">
        <property type="nucleotide sequence ID" value="XM_009266498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUD0"/>
    <dbReference type="GeneID" id="20521584"/>
    <dbReference type="KEGG" id="ero:EROM_070250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070250"/>
    <dbReference type="HOGENOM" id="CLU_005316_7_4_1"/>
    <dbReference type="OrthoDB" id="435282at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF4">
        <property type="entry name" value="28S RRNA (CYTOSINE-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="6">
        <location>
            <begin position="86"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="299"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="184"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="365" mass="42544" checksum="49643B8E64891645" modified="2012-10-03" version="1">MKKEESSKSRFDRRCVEMVRNVLEGRSTIKGEVYKTRNPKMYHRLVHMVVENYELLLEVIKKCKYMEDDRELGVVRCFEILDRRIRNTRMYKKFMKALDGKKLKLTKPCVFVRINTLKNGTERDIEFLSPKRTCVEGVYKIVDSKDMIFSQEYKDGKFVIQNISSCLPAYILNPKECSRVIDTCSAPGNKTSQLSMIMKNTGRIYAFERSETRAKTLQDQLERLGVSNVEVIENDFMKASPDDFKDIQYILCDPSCSGSGMHLGYKMDEKRIESLKLFQIAIVEHALRFKPERLVYSVCSDHKEEGEEVVDEVLKNSDYELEDISTFWSSSCPFEFPFSHKVIRCKRNDNGATGFFIALFVKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUE6</accession>
    <name>I6ZUE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264793.1">
        <property type="nucleotide sequence ID" value="XM_009266518.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUE6"/>
    <dbReference type="GeneID" id="20521604"/>
    <dbReference type="KEGG" id="ero:EROM_070450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070450"/>
    <dbReference type="HOGENOM" id="CLU_1441049_0_0_1"/>
    <dbReference type="OrthoDB" id="2190291at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031539">
        <property type="entry name" value="DUF5090"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17009">
        <property type="entry name" value="DUF5090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="187" mass="21170" checksum="B78F60043B720D83" modified="2012-10-03" version="1">MMEGQQEKVLDAANVVEAKAMSAIDNVEKSSLGNSIPLEVSLPLRELTKYLMLFTIFESIPVYLIFCEGNKNEFLHWISKGTGLIFLVIAIIAGILDLVTFYGKMFNLYKFVVPATLAKTALSFLVVVFSNISSMYVVFIAILYSLRIALFDALFLYYLAILLRRIESDDYDNNGERLRKDVRGEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUL4</accession>
    <name>I6ZUL4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_071400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264888.1">
        <property type="nucleotide sequence ID" value="XM_009266613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUL4"/>
    <dbReference type="GeneID" id="20521700"/>
    <dbReference type="KEGG" id="ero:EROM_071400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071400"/>
    <dbReference type="HOGENOM" id="CLU_1255982_0_0_1"/>
    <dbReference type="OrthoDB" id="2192150at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="233" mass="26587" checksum="BF9971785110CC3D" modified="2012-10-03" version="1">MNLLLFGYAAGQMVAYESERILERLNGLDVTKTVSDGRLDLHLYFREIEDDMLDPPFPLLMQRLKSISAFAIEKGGKAYRIVPFESIEIGFENGKKSYGKFNDIVSMGNEAVVTYTCNGNDAEEGIYSTITIIFTPSDEEEDKIEKIYSGWSDSCLLRVSTPKLKVWYKRRILSMYLEDFGNKELDGKDIGHWVDGINSDGERVDGDRILKEEMMVFEEAGSKEREELSRKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUS0</accession>
    <name>I6ZUS0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="4">PPIase</shortName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_080390</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264958.1">
        <property type="nucleotide sequence ID" value="XM_009266683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUS0"/>
    <dbReference type="GeneID" id="20521775"/>
    <dbReference type="KEGG" id="ero:EROM_080390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080390"/>
    <dbReference type="HOGENOM" id="CLU_012062_4_2_1"/>
    <dbReference type="OrthoDB" id="193499at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000013">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024936">
        <property type="entry name" value="Cyclophilin-type_PPIase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001467">
        <property type="entry name" value="Peptidylpro_ismrse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="5">
        <location>
            <begin position="21"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="183" mass="20711" checksum="5037412883834AA0" modified="2012-10-03" version="1">MSEKNDENTHSNVAEENKNVYFDVYANEERLGRIVMKLFDDVVPRTARNFRELCKRPERAGYKGSIFHRIIPGFMVQGGDYTAHNGTGGESIYGDRFDDENFNLKHSKEGILSMANCGKHTNGSQFFITLEKTPWLDGKHVVFGEVIEGMSVVHRIAKYGSQNGQVARGYKVEIRDCGILETN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUW1</accession>
    <name>I6ZUW1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Elongator complex protein 3</fullName>
            <ecNumber evidence="15">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_081100</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalytic tRNA acetyltransferase subunit of the elongator complex, which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="15 16">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="15"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265023.1">
        <property type="nucleotide sequence ID" value="XM_009266748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUW1"/>
    <dbReference type="GeneID" id="20521845"/>
    <dbReference type="KEGG" id="ero:EROM_081100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081100"/>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_1"/>
    <dbReference type="OrthoDB" id="10265243at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13 15" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 16" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="425"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR005669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN83526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="560" mass="63610" checksum="4074DB7A3C878E49" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV37</accession>
    <name>I6ZV37_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265108.1">
        <property type="nucleotide sequence ID" value="XM_009266833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV37"/>
    <dbReference type="GeneID" id="20521932"/>
    <dbReference type="KEGG" id="ero:EROM_081970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081970"/>
    <dbReference type="HOGENOM" id="CLU_1562865_0_0_1"/>
    <dbReference type="OrthoDB" id="2191172at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="172" mass="20454" checksum="DCD586800DB3FA95" modified="2012-10-03" version="1">MDDLKVKIDSLTREVRNLSKELQKGYEEIKKLRARTREQKEGIMQRDSRIDELNMCISKLKKRIDEKTKDVGSREKAIINESRRKELFNEKVLGSSRSQGEYYISLEMKILNTRLEIMRRFILEISKRFGFDFEVFDELTKISENFDDPVIYAFLNSVSPNKQSSQEKKEME</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV78</accession>
    <name>I6ZV78_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">HrpA-like helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_090130</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265128.1">
        <property type="nucleotide sequence ID" value="XM_009266853.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV78"/>
    <dbReference type="GeneID" id="20521952"/>
    <dbReference type="KEGG" id="ero:EROM_090130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090130"/>
    <dbReference type="HOGENOM" id="CLU_001832_5_11_1"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17917">
        <property type="entry name" value="DEXHc_RHA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF91">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE PRP16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="116"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="288"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="798" mass="90833" checksum="6AA0F437BE2EAFB8" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVP1</accession>
    <name>I6ZVP1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265308.1">
        <property type="nucleotide sequence ID" value="XM_009267033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVP1"/>
    <dbReference type="GeneID" id="20564421"/>
    <dbReference type="KEGG" id="ero:EROM_091970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091970"/>
    <dbReference type="HOGENOM" id="CLU_606945_0_0_1"/>
    <dbReference type="OrthoDB" id="2021145at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="451" mass="53362" checksum="8FC68B2934EBB5E9" modified="2012-10-03" version="1">MVFFHKNAWALMLKELIDEDDFCRKVRMSKEANTKTGFYSLFYILLHDPSLQVLESLLSNYTLQDVVNGLKHLIKFKFDRLTKEAYENILLVVQMMLGYEENIDTLIINLLRHHLVEEVYSMYKDNPKYFSNHPNSLMFLVFFCAQSNSKRDVFEDSNFTTRDFLFTTVKEMVTNPHFDEYKKKRAKKELRDKNPEYPVFPFTPYFSSRQLLSTLFTPTPLNILSSQLSADLEMNIMFILEHDQNFEFHLSLLFKNYIRSEDDACRVLRFMVNIPYPQNIGRLANSLLDKYPSSYLALIYDMLFWNQKERLNGNLIEYLRGNSNRIAAKLDSLQEEWGALKLAIKSTKKKQEHTVEDVTRMIHSLNFQKYFGLVKEMEKWGTPIVPKEMYDRIIEDSSDWDGYAQVYLWKIIGFQKIYLGFDVEVPKRVESLEALEGLEIVRNLSSMEKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVP5</accession>
    <name>I6ZVP5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_092020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265313.1">
        <property type="nucleotide sequence ID" value="XM_009267038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVP5"/>
    <dbReference type="GeneID" id="20564426"/>
    <dbReference type="KEGG" id="ero:EROM_092020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_092020"/>
    <dbReference type="HOGENOM" id="CLU_1384154_0_0_1"/>
    <dbReference type="OrthoDB" id="2190213at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="197" mass="22679" checksum="D468269ADDA54E7D" modified="2012-10-03" version="1">MNSESSSTVLASIQSKYKFRAKTLEYSTAFYPDRFMILYKDRKAGEIPYENILRVEKNREGLKFGLKNGTWVKIPCTPDEKRKIFRIFKTKKLGGILSESDIIGLALVEFADLRFLVPLGSTCFADFKKNVIRRIGGHFLPTQSLDFVSLDHYNEFSLYIKDKDSLVIFENDDDLLCSLLYFEKKLAVVVKHVPKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVU1</accession>
    <name>I6ZVU1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CCT-eta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_100500</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265363.1">
        <property type="nucleotide sequence ID" value="XM_009267088.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVU1"/>
    <dbReference type="GeneID" id="20564480"/>
    <dbReference type="KEGG" id="ero:EROM_100500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100500"/>
    <dbReference type="HOGENOM" id="CLU_008891_7_1_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="511" mass="56372" checksum="6121F6AC9D74566F" modified="2012-10-03" version="1">MNQLFVQTEKVADPREGKLQVVSNIDVCIKIAEFLESTLGPYGMDKLFAGKEIVVTNDGATILKHMKVKHPVGKLLVGISESQDSEVGDGTTSVVVLTAEILNCLKSLIKDSFDLESIRECLSELRAACIEHVEKMSVEVDDEMLYKLAETCVTSKNIRHEKKHFSRMVVDAVRQSRVDDMESIGIKKVQGGSIGDSIAVNGVAFEKCFTYAGYEQQPKRISNPKILCLNVELEWKSERDNAEMRVGGVEEYQKVVNAEWTIISRKLDEIIDSGANVVLSSLSIGDYATQYFAKHGIFCAGRVSKEDLGRVVGSCGGRILGSTDYLEDSLGSCELFEERQLGKLRYNYFEGGGMNACTIILRGPGQEVLEEIGRAVHDAICVVRTALKTRRAVSGGGSVEMELSKMIREKSMEYGNKKVFVAKAVGQAFEKIPLLLAKNFGFDTISIIQDLRKKHANGSVYQGVSIEGVRDMRDLGIYEPLEVKKNMIKASFNGAASIIMIDSTIMAERSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWC3</accession>
    <name>I6ZWC3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Dcp1-like decapping protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_110840</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DCP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265563.1">
        <property type="nucleotide sequence ID" value="XM_009267288.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWC3"/>
    <dbReference type="GeneID" id="20564683"/>
    <dbReference type="KEGG" id="ero:EROM_110840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110840"/>
    <dbReference type="HOGENOM" id="CLU_141163_0_0_1"/>
    <dbReference type="OrthoDB" id="440673at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010334">
        <property type="entry name" value="Dcp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290:SF0">
        <property type="entry name" value="DECAPPING PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16290">
        <property type="entry name" value="TRANSCRIPTION FACTOR SMIF DECAPPING ENZYME DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06058">
        <property type="entry name" value="DCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="122" mass="14380" checksum="62014D21AA7F6E93" modified="2012-10-03" version="1">MLGEREVQILAREIRKEDRLFKDLLFVSNFASVYHYSGEWDKLNIEGTFVMYSRACHPFVGIYVFNRKSLKDFCLHLTKETSFGVKKNFMTINKGGMDGTHGLWFHNDSHPQEVLKRLEEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACQ7</accession>
    <name>I7ACQ7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spore wall protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263862.1">
        <property type="nucleotide sequence ID" value="XM_009265587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACQ7"/>
    <dbReference type="GeneID" id="20520647"/>
    <dbReference type="KEGG" id="ero:EROM_010200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010200"/>
    <dbReference type="HOGENOM" id="CLU_098346_0_0_1"/>
    <dbReference type="OrthoDB" id="2194161at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018859">
        <property type="entry name" value="BAR_dom-cont"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10455">
        <property type="entry name" value="BAR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27149" checksum="69D5C01F3157E386" modified="2012-10-03" version="1">MDKETVKDIQRSIAMKLNRVDYTEVSLISGYSEAEDCYKKFRDNLKKISDSITWLMTYEHGGSKMKSLYSKMDMITSTSRLGHFKNYDIYELNGLVGLELSRIGLGSNVSDIGKKYSKAHMDISRYKVEMNRKLEQQIKKISELRDHSNAIDKKRKKISNIRYDLEMMKRSKDPKAPDAVSKEEEMERTFKETSKEALKEMERFIGNDGVCGVLQSVAQAHGEFSEKSSKVLGEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACU3</accession>
    <name>I7ACU3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Spore wall and anchoring disk complex protein EnP1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263902.1">
        <property type="nucleotide sequence ID" value="XM_009265627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACU3"/>
    <dbReference type="GeneID" id="20520688"/>
    <dbReference type="KEGG" id="ero:EROM_010610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010610"/>
    <dbReference type="HOGENOM" id="CLU_715897_0_0_1"/>
    <dbReference type="OrthoDB" id="2191612at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707765" evidence="1">
        <location>
            <begin position="17"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="348" mass="39519" checksum="D6FF3F307332FE45" modified="2012-10-03" version="1">MKLLGLLAIAFGAINALKTKALHLNCEQELKPYNAVLDAKCMAFALNGSNINEAIRYLKAMNIEKAYVLYWNDHDLRQNPMVLYSNGTLAPLDPHTNTAKYVLCVETCTCPGSEIRPSAPAPCEKIYIENNGSVPCEKIYIGGETTTCKKIVVNPSPKRECIPCEPCDSSSSDEVCKDKRCKVFPRVCKKACGSRHRRSPKKVEVVKSQKTFTFDVEKYRRRGEVVVRVCSKDSKDKFEKFVLSRNGEIRGKNDKCVPEVLPKCLRCPSKVYKLKKRIERDVCQKVCMYINSKCEIFVLIGDCEFYRVIVRENGRHRGLRLRKVRGHKLKELIKHGLFGVEFGPVDCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD13</accession>
    <name>I7AD13_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">WD40 repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_020150</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat DCAF13/WDSOF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263987.1">
        <property type="nucleotide sequence ID" value="XM_009265712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD13"/>
    <dbReference type="GeneID" id="20520773"/>
    <dbReference type="KEGG" id="ero:EROM_020150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020150"/>
    <dbReference type="HOGENOM" id="CLU_033999_0_0_1"/>
    <dbReference type="OrthoDB" id="10249065at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007287">
        <property type="entry name" value="Sof1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051733">
        <property type="entry name" value="WD_repeat_DCAF13/WDSOF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851:SF0">
        <property type="entry name" value="DDB1- AND CUL4-ASSOCIATED FACTOR 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22851">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA U3 SNORNA ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04158">
        <property type="entry name" value="Sof1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="62"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="301"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Sof1-like protein" evidence="8">
        <location>
            <begin position="335"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="405" mass="46411" checksum="60179DD8082AAFD3" modified="2012-10-03" version="1">MKINTIYHKPEEICKERKKDVEYMSFAKDEAYHPFMKEREFVRALNSVKIERMLSKPFITALSYHKEGIHLLARHFQQPLFASGSFDNQVVLWDLGQRSVLRRFECNSSIKGLGMDIEGNLYVGQEKEVKRLGSGQTYRCNSEVVNLDIMGTLNVGTSRSIEIFDIERDFSKQQMSTRYPLCISTSAVLTSIIGVGEQGGLSLFDIRVGKVVHSVSVGSKTNDISFSPMDGHIFVSGNEDFCTYLHDIRYLHEPSGIYRGHGNAVVSVEFNPPGTEIATGSFDKTIRIFDVNERKSRDTYYNKRMHNVFGVKYSHDSQFIVSGSDDGSIRVWKSYASKKLGPLSRKEKDALRYSEILKEKYKEVGEISRISKHRFLPKPLKNTLKTIYESHKARERKRKARESEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD42</accession>
    <name>I7AD42_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_020450</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264017.1">
        <property type="nucleotide sequence ID" value="XM_009265742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD42"/>
    <dbReference type="GeneID" id="20520804"/>
    <dbReference type="KEGG" id="ero:EROM_020450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020450"/>
    <dbReference type="HOGENOM" id="CLU_012062_25_3_1"/>
    <dbReference type="OrthoDB" id="275748at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="3">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="93" mass="11001" checksum="83F560D5D456D542" modified="2012-10-03" version="1">MEQIDTQILFVRNLPKDVSKDKIIELFEEYGTIIQIRVGVEKNTAGNAFVGYDRVESARKAVRHMNGYYLGDKYLNIGYWQPFDKFRFMMGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD68</accession>
    <name>I7AD68_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor IIF subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">TFIIF medium subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">TFIIF-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_020700</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIF beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264042.1">
        <property type="nucleotide sequence ID" value="XM_009265767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD68"/>
    <dbReference type="GeneID" id="20520830"/>
    <dbReference type="KEGG" id="ero:EROM_020700"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020700"/>
    <dbReference type="HOGENOM" id="CLU_047858_0_0_1"/>
    <dbReference type="OrthoDB" id="26094at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000035">
        <property type="entry name" value="General transcription factor IIF subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003196">
        <property type="entry name" value="TFIIF_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040450">
        <property type="entry name" value="TFIIF_beta_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040504">
        <property type="entry name" value="TFIIF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10445:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02270">
        <property type="entry name" value="TFIIF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17683">
        <property type="entry name" value="TFIIF_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFIIF beta subunit N-terminal" evidence="11">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIF beta subunit HTH" evidence="10">
        <location>
            <begin position="165"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="228" mass="26377" checksum="19713CF6D6105439" modified="2012-10-03" version="1">MELNINNKNTKIWLAKVPLFLAERILSRNEETVVGELDITKATPTEPAVLNLKLSKEFCEGGFPSSFDVKIKARDNNMYVVKAHENNADVEGMINNECYITPEINEEYLKYKKDAGFKSSAKKGDVQVIDYLKEGKRGEKFGSLRELEYLARKRKKMLMDKKRERLGKNEVIDMVFKAFEKYPSWTVKDLADFCGQPVAFIQEIVSDICVLNKKDLKNTYELRPEYKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADQ4</accession>
    <name>I7ADQ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264202.1">
        <property type="nucleotide sequence ID" value="XM_009265927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADQ4"/>
    <dbReference type="GeneID" id="20520995"/>
    <dbReference type="KEGG" id="ero:EROM_030840"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030840"/>
    <dbReference type="HOGENOM" id="CLU_047023_0_0_1"/>
    <dbReference type="OrthoDB" id="2195759at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="466" mass="54546" checksum="BFAE7BCDD243664C" modified="2012-10-03" version="1">MKQLDFLRRIVSGQRDLEKEFVQALLRSDVQKSIGLGKMLFSRNPMFLVTSLLIDFLGNPGDEIKKDLFLESLKDATIKSNLIWMLYKRGLLIEEMYHYVQGITFKDHLYYLVLKEACIHGHHKLLGKKDGLECVEFLLDSLDDWDLYKYALDNKIEVQNRESLNYEYYLLHKLKEKGRAVELLKSRTCFREIEFIAEMVGLESHPHEAIDCTIQLMRKGFDEGLLRRAYEVYRRDMSVFNTKVVIAILIASRKASFLALALYLSFRHRNSYQGNYEIFLIFTFLCRYFWFYPYVLKCLECMNVKNAQIPNLSFIWSDILITKGIKDEKRRIGAIINFQESINDLDNSIKYFIIVGNLAHVVDALELRKSIEKSVILSELKESRIIGTNGSNSFHQLLGTRCSYLFEKMTVGKMPKGRGMFLTDFYVSDSCTLDEVLNNGLCKVEEDFVAFFKEMVKYQEYMNKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE86</accession>
    <name>I7AE86_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Phosphomannomutase</fullName>
            <ecNumber evidence="5 13">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_050220</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="5 13"/>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264452.1">
        <property type="nucleotide sequence ID" value="XM_009266177.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE86"/>
    <dbReference type="GeneID" id="20521254"/>
    <dbReference type="KEGG" id="ero:EROM_050220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050220"/>
    <dbReference type="HOGENOM" id="CLU_065642_0_1_1"/>
    <dbReference type="OrthoDB" id="10264771at2759"/>
    <dbReference type="UniPathway" id="UPA00126">
        <property type="reaction ID" value="UER00424"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0413">Isomerase</keyword>
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    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="12"/>
        </location>
    </feature>
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        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
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        <location>
            <position position="21"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR605002-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="256" mass="29828" checksum="774FCE166EF07281" modified="2012-10-03" version="1">MARDEKTIFLFDVDGTLSESRASMTEKMREMLKKLKKKVHIGFVGGSDLEKQREQIGDDLLELFEYGFPENGVSFYKNGTLKSQEKIINVLGEKFYKEFVGFVLEYLSKMDIPIKRGNFIEYRNSMINISPIGRNCSREERKEFFELDKKEKFRKSMVAAMRERFKNSCLVFSIGGQISIDCFPKGWDKTYCLRHIRDEGIKNVYFFGDMTMEGGNDYEIFNHQDVHGVTVENPDDTYRKVSQKLKEIGLGDLNED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEL4</accession>
    <name>I7AEL4_ENCRO</name>
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        <recommendedName>
            <fullName evidence="3">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ordered locus">EROM_060200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
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        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725:SF14">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15725">
        <property type="entry name" value="ZN-FINGER, C-X8-C-X5-C-X3-H TYPE-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15663">
        <property type="entry name" value="zf-CCCH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="3">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="1">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="113"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="234" mass="27278" checksum="99B0AFC80ECA741F" modified="2012-10-03" version="1">MDDCYFFLYSSCKFKDRCTYRHNEGCRNNLVTCKNWESKIPCSEDCPYRHSKFHLKKNRREEMCYWEDKPGGCTKDRCEYRHTDPTKDAWKTRASSEEPARLRPSISPSLGSEGHHISEDTRLKDYEEVMSIQDKPTGSKDSASASKEFAEEENKEDQDSHENSVLPKKDEDIDIPSKIELKNNVLERSPSENTQEPQNKRVKTEDPLDTISKTVVNLEELDKEIEELDSILMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF96</accession>
    <name>I7AF96_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264862.1">
        <property type="nucleotide sequence ID" value="XM_009266587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF96"/>
    <dbReference type="GeneID" id="20521674"/>
    <dbReference type="KEGG" id="ero:EROM_071140"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071140"/>
    <dbReference type="HOGENOM" id="CLU_1992612_0_0_1"/>
    <dbReference type="OrthoDB" id="2192725at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="125" mass="14267" checksum="812A560A6D62231A" modified="2012-10-03" version="1">MEEERTVEKVKEMLQRYSSRGGTFSEGSFLSDIDAPSDISFTSWEEELTGRIIKSLKYCKEMTEMARRRKGCISRKEVIECMSILDSIKRNIEEPREVRKGWIILAVSVGIGLIGIAYVSAKHCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFH0</accession>
    <name>I7AFH0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080380</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264957.1">
        <property type="nucleotide sequence ID" value="XM_009266682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFH0"/>
    <dbReference type="GeneID" id="20521774"/>
    <dbReference type="KEGG" id="ero:EROM_080380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080380"/>
    <dbReference type="HOGENOM" id="CLU_081901_0_0_1"/>
    <dbReference type="OrthoDB" id="10252231at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="180" mass="20822" checksum="550AFE8FCC7028C5" modified="2012-10-03" version="1">MEKELYDLYDRQIRLFGKDTQKLIEESHVAIIQGPPYVLGREKRINDVGGEILKNLVLLGVERVTVNKHVISSFKRMFVNEITKINEKIRVAVIDEQQTEEAWNGYTLVILIDCKGQTTCPGIYVCSRCMGFHPLGKRHLCRECSNISVVHDCLLGAIIVQEWVKRLQKKPFVDEYYLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFH5</accession>
    <name>I7AFH5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_080480</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264967.1">
        <property type="nucleotide sequence ID" value="XM_009266692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFH5"/>
    <dbReference type="GeneID" id="20521784"/>
    <dbReference type="KEGG" id="ero:EROM_080480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080480"/>
    <dbReference type="HOGENOM" id="CLU_006406_6_2_1"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="445"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="563" mass="64438" checksum="1CFAA733BC9DB572" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFK7</accession>
    <name>I7AFK7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHF5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265012.1">
        <property type="nucleotide sequence ID" value="XM_009266737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFK7"/>
    <dbReference type="GeneID" id="20521834"/>
    <dbReference type="KEGG" id="ero:EROM_081000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081000"/>
    <dbReference type="HOGENOM" id="CLU_2441450_0_0_1"/>
    <dbReference type="OrthoDB" id="10248186at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005345">
        <property type="entry name" value="PHF5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13120">
        <property type="entry name" value="PHD FINGER-LIKE DOMAIN-CONTAINING PROTEIN 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03660">
        <property type="entry name" value="PHF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="90" mass="10371" checksum="7AF6EA9BE0229205" modified="2012-10-03" version="1">MYLHMKSRCGKISTGSVAMMCDKCSGRCYICGLDAGTSSRRIYICSVCFHSMYKDRCIVCKLKDPRNNAYCCRECWLLQKNHDRCPVLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFZ5</accession>
    <name>I7AFZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265137.1">
        <property type="nucleotide sequence ID" value="XM_009266862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFZ5"/>
    <dbReference type="GeneID" id="20521961"/>
    <dbReference type="KEGG" id="ero:EROM_090220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090220"/>
    <dbReference type="HOGENOM" id="CLU_082796_0_0_1"/>
    <dbReference type="OrthoDB" id="2190512at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031515">
        <property type="entry name" value="DUF5095"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17016">
        <property type="entry name" value="DUF5095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="281" mass="32495" checksum="D8F2573610E17EEF" modified="2012-10-03" version="1">MENTSLNVGVEGVLGSGAKGKNGQGTKRRCGMDGKINALSKISDNPFDVLSQSLYKKNEISCKEVPSKQDLMEDSIFEELLLKAKTEPKNEEKKLCYGIFTSMALVTDSEIQIQEKRMYKFPFEVETKNDGSQLFEKMYSEYLIAAKSVFREYKRSSIRFFMKLKNDFLLFDSYLKTSYGMKKELVRNEIRFEEEGSFLIVKGIEVALVFDYIINIPISSSFCIPFILSESQFENGILFSVKLERKRAIREGKKILYSYELLGPFWADDYKELIRFHTKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG76</accession>
    <name>I7AG76_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Serine/threonine-protein phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_091390</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PPP phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265252.1">
        <property type="nucleotide sequence ID" value="XM_009266977.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG76"/>
    <dbReference type="GeneID" id="20564363"/>
    <dbReference type="KEGG" id="ero:EROM_091390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091390"/>
    <dbReference type="HOGENOM" id="CLU_004962_8_1_1"/>
    <dbReference type="OrthoDB" id="1930084at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047129">
        <property type="entry name" value="PPA2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006186">
        <property type="entry name" value="Ser/Thr-sp_prot-phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45619">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE PP2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00114">
        <property type="entry name" value="STPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00156">
        <property type="entry name" value="PP2Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00125">
        <property type="entry name" value="SER_THR_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0464">Manganese</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine/threonine specific protein phosphatases" evidence="6">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="302" mass="34554" checksum="F91100107B52B402" modified="2012-10-03" version="1">MKKFVSRLLNLELLSEVEIQDICNKVSEILISEPNVPNKKTPVIVCGDIHGQFFDLVSLFRIDGIPSGKRYVFLGDYVDRGKNSLECILFLFLLKILYPENVTLIRGNHEQSTINRVYGFYEEIIEKYGNPRIWRMINEVFNLMCVGCLVDGRILCVHGGISPKIMSLNQMRRLDRLLPIGEGSEFADILWGDPYEGKGFQPSPRGSGYLYGEDVVIRFLELNGLTSIVRSHQLVIEGYKFHFPERNVITVWSAPNYMGKCGNPASILRINETLDVSDKDFCIFKSTPQKKEFLERLEGFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGQ5</accession>
    <name>I7AGQ5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Meiotic nuclear division protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_101500</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for proper homologous chromosome pairing and efficient cross-over and intragenic recombination during meiosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MND1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265462.1">
        <property type="nucleotide sequence ID" value="XM_009267187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGQ5"/>
    <dbReference type="GeneID" id="20564580"/>
    <dbReference type="KEGG" id="ero:EROM_101500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101500"/>
    <dbReference type="HOGENOM" id="CLU_080628_3_1_1"/>
    <dbReference type="OrthoDB" id="273345at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007131">
        <property type="term" value="P:reciprocal meiotic recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040661">
        <property type="entry name" value="LZ3wCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005647">
        <property type="entry name" value="Mnd1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040453">
        <property type="entry name" value="Mnd1_HTH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18517">
        <property type="entry name" value="LZ3wCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03962">
        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026991">
        <property type="entry name" value="Mnd1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mnd1 HTH" evidence="7">
        <location>
            <begin position="15"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Leucine zipper with capping helix" evidence="8">
        <location>
            <begin position="161"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="77"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR026991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="203" mass="23762" checksum="0F046BA8F10335EB" modified="2012-10-03" version="1">MATVKMKADQKKTILLEIIRGSRSFFKLQELENLGSKKGIVANTIKEILQQLVDDGLVTVEKVGTSNLYWSFASEGVQKKNLKFKELVEECKSMSEEILKKKKHLENERISKNYTEERKELESKLNALAKIEEEQKEELSKFEETDPTIYDKFVDDRKEIVDEYNKIIDNIFIIQDYVCNKFSMEKPEFNSNFGIPQDLDYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGX2</accession>
    <name>I7AGX2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome biogenesis protein NSA2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_110680</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the pre-66S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the eukaryotic ribosomal protein eS8 family. Ribosome biogenesis protein NSA2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265547.1">
        <property type="nucleotide sequence ID" value="XM_009267272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGX2"/>
    <dbReference type="GeneID" id="20564667"/>
    <dbReference type="KEGG" id="ero:EROM_110680"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110680"/>
    <dbReference type="HOGENOM" id="CLU_1070048_0_0_1"/>
    <dbReference type="OrthoDB" id="1847590at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030684">
        <property type="term" value="C:preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11381">
        <property type="entry name" value="NSA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000001">
        <property type="entry name" value="NSA2, ribosome biogenesis homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039411">
        <property type="entry name" value="NSA2_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12642">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN NSA2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN84050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="258" mass="29786" checksum="328165D88F7FDB16" modified="2012-10-03" version="1">MPQNDFVERHIKKYGRRLDYEIRKYKKERREEKVVAKKANKLFGIKAKLFAKKQRAAKIQLKKDMKMRESKKRNIEISAESNPLPHFLLDRELQEKAKDINDKIKQQRQEKGSKYSVPLPKIHPLPEKEVFGVVVSGKRKSKHWKRMVTKPCFVGSDFTRKPPKYERFIRPMAMRFKTAHVSHPELNSTFNLKIIGLKKNPHSDVYTGLGILSKGTIIEVDVSPLGLVNGSGKIIWGKYAQITNNPENDGCVNAVLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH27</accession>
    <name>I7AH27_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_111290</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265607.1">
        <property type="nucleotide sequence ID" value="XM_009267332.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH27"/>
    <dbReference type="GeneID" id="20564728"/>
    <dbReference type="KEGG" id="ero:EROM_111290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111290"/>
    <dbReference type="HOGENOM" id="CLU_093932_0_1_1"/>
    <dbReference type="OrthoDB" id="282270at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001193">
        <property type="term" value="P:maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006283">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="11">
        <location>
            <begin position="68"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN84110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="108" mass="12514" checksum="E304AC8E97451AE7" modified="2012-10-03" version="1">MGDRFCKECSNLLYPKEDDSTLFLACKSCEHIQEAKSHRLYAKNFRPKHTSIELYAKDLAQDPTLPSIKIDCKNCGFNKGLYFQSRGGEDDVALNIYYLCCRCHHLWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALD4</accession>
    <name>I7ALD4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_020040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263976.1">
        <property type="nucleotide sequence ID" value="XM_009265701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALD4"/>
    <dbReference type="GeneID" id="20520762"/>
    <dbReference type="KEGG" id="ero:EROM_020040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020040"/>
    <dbReference type="HOGENOM" id="CLU_950031_0_0_1"/>
    <dbReference type="OrthoDB" id="2404674at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="293" mass="34567" checksum="37238E2BE8A6F7C9" modified="2012-10-03" version="1">MDLGRYLGSVLENTERTLKVARLEKEIENRCRVQRDLIFSQRHIDYQMKNKEMKARMELEICKILRKFHEWILKQNLRYTKAISSKTQDSGDRVGSKGKGSNESMEIVYCKDQISTLLSHLYNNVNMGVRLLNFQKACEYSNRVKNIRSDVMYLINKLGLNILKGIEKVIRDYNNSMNEKEDIILECEDLKIKSLLGQLRQKVNEYEEIIREEAISRVLAVLSQGSVENDIRFAKLIDETLERTEGYESPEITRMSVQFIIAKKYVEDVVSLKEQPFLRRYLERNTRPKEQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALJ8</accession>
    <name>I7ALJ8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264046.1">
        <property type="nucleotide sequence ID" value="XM_009265771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALJ8"/>
    <dbReference type="GeneID" id="20520834"/>
    <dbReference type="KEGG" id="ero:EROM_020740"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020740"/>
    <dbReference type="HOGENOM" id="CLU_2121041_0_0_1"/>
    <dbReference type="OrthoDB" id="2193737at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="14168" checksum="702FDDDF3B41EACE" modified="2012-10-03" version="1">MRYFCHEILLLEEIIKGLLDSEEIEETTARKYKSLLEVKDKINSRMIEMEEYYMKRIEFYRECDGAKVKELEKDVRSRDEKYLGLLVKAKELRNKIERLEVRNKLFRLKSESRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM83</accession>
    <name>I7AM83_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_040300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264296.1">
        <property type="nucleotide sequence ID" value="XM_009266021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AM83"/>
    <dbReference type="GeneID" id="20521094"/>
    <dbReference type="KEGG" id="ero:EROM_040300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040300"/>
    <dbReference type="HOGENOM" id="CLU_2291682_0_0_1"/>
    <dbReference type="OrthoDB" id="2189271at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="101" mass="11702" checksum="031723380A06106D" modified="2012-10-03" version="1">MEKIELVKSIEANAERILEDFTEILQYLRSEGSDKCDEVQNLLFATRRVVNLVDSALEIYHYLIRLKYLTADIQRFETKESGDGDKMLEELFGELNSNLGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANM8</accession>
    <name>I7ANM8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264881.1">
        <property type="nucleotide sequence ID" value="XM_009266606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANM8"/>
    <dbReference type="GeneID" id="20521693"/>
    <dbReference type="KEGG" id="ero:EROM_071330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071330"/>
    <dbReference type="HOGENOM" id="CLU_2960731_0_0_1"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="59" mass="6976" checksum="0104FD1E3D1F3256" modified="2012-10-03" version="1">MKCMFTFTYARFALYGMLTLYAHLIKALLTYPTYVTFYVPSPVVNGIDLLDFYFHHSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANW1</accession>
    <name>I7ANW1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nicotinic acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264976.1">
        <property type="nucleotide sequence ID" value="XM_009266701.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANW1"/>
    <dbReference type="GeneID" id="20521795"/>
    <dbReference type="KEGG" id="ero:EROM_080590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080590"/>
    <dbReference type="HOGENOM" id="CLU_930747_0_0_1"/>
    <dbReference type="OrthoDB" id="2191207at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="299" mass="33747" checksum="CE160334FCD1DF6B" modified="2012-10-03" version="1">MWSKKKLEAFVILNIVAAVILSVLKVVVLVNYKAGGLLMLVSSFAKMMISYLNIQSFDFKMKYAFIIAGLSFIGGYLGMVSYYYKASSISYVISSDLQYLFTALIATAWNGTRYSSTQCVGILMILGGFFIEISSGERFSIPFHMLIGTLSGLFNSISLAIFESKIKPALVDFRRFWCYMTTYTFFLTFFSLFYAAEEVFSKKSNYVKIISSPIIYIVFLIEVLTTYLLSEISLFLDTVERSTLLNVVTGISAVVSDVYLSREIDVQRFLAFSFTAIGVQIFNFFWKEAVHPFGQACTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANZ0</accession>
    <name>I7ANZ0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GLTSCR protein conserved domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265011.1">
        <property type="nucleotide sequence ID" value="XM_009266736.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANZ0"/>
    <dbReference type="GeneID" id="20521833"/>
    <dbReference type="KEGG" id="ero:EROM_080990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080990"/>
    <dbReference type="HOGENOM" id="CLU_1165804_0_0_1"/>
    <dbReference type="OrthoDB" id="2556847at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27526" checksum="BCF3CC64B4EA614D" modified="2012-10-03" version="1">MERASDRPDGVDSGMDDLNGAEPFEGGDLISSDVRDTSQTREAFEPHGMMKTKGVRGPYKKAKRVEEGGYGYLYGNLYEQAQARRQKILGFLQKDLFQIGRPDLRRFMNVNHAYECLLPYHIFGFDLYEDMLFMSSKEAGGISSEIDEMIELMKDAVVLESRPVVVEETLVCELLLYYQQRYINSIYGDGKKQKAQRRYRQQLNKRNTVFRLKIGVREPRGNDVNVRNGRLYFKKYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP41</accession>
    <name>I7AP41_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Proteasome subunit alpha type-2-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265071.1">
        <property type="nucleotide sequence ID" value="XM_009266796.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP41"/>
    <dbReference type="GeneID" id="20521893"/>
    <dbReference type="KEGG" id="ero:EROM_081580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081580"/>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_1"/>
    <dbReference type="OrthoDB" id="431557at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="233" mass="25810" checksum="A9A293A5E23C0454" modified="2012-10-03" version="1">MDFGREKPVTLFSSEGNLGQCDNALRAAANGSLSIGASAADGVVLMSFRDVPPLVIKEEVKKVFKICDTIGCTYSGLQPDCRIQVNIGCQIAEEYYDVYGRYPDVDVFTAHFSSVVQEYTQKSGYRPFGTLMIFAGPVKGVPRLYQVDPSGSYQTADVCTSGIGYSEAKKFIKRRLESLDDNMVNCVSSMREFAGKEVHPEDIDIGVYYKTKNVFKTFTLDEVKEVFDSLSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AP92</accession>
    <name>I7AP92_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265121.1">
        <property type="nucleotide sequence ID" value="XM_009266846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AP92"/>
    <dbReference type="GeneID" id="20521945"/>
    <dbReference type="KEGG" id="ero:EROM_090060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090060"/>
    <dbReference type="HOGENOM" id="CLU_1855257_0_0_1"/>
    <dbReference type="OrthoDB" id="2190384at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="138" mass="16467" checksum="02E904EAF4E390F3" modified="2012-10-03" version="1">MNQDMIVKIMPFLAKRSILVLHYITRDPKYPIYVINGYKSKLNITPIGKITMLIQKAIDIERNDLAIEYLCHVSPGRMTRILFRLTENMEPEKIQHMISDLKRNHFCLLCNFNNRCEEVITHLEITIKNMRLYTYYCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7APA5</accession>
    <name>I7APA5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF5096 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265141.1">
        <property type="nucleotide sequence ID" value="XM_009266866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APA5"/>
    <dbReference type="GeneID" id="20521965"/>
    <dbReference type="KEGG" id="ero:EROM_090270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090270"/>
    <dbReference type="HOGENOM" id="CLU_804352_0_0_1"/>
    <dbReference type="OrthoDB" id="2192089at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031512">
        <property type="entry name" value="DUF5096"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17019">
        <property type="entry name" value="DUF5096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5096" evidence="2">
        <location>
            <begin position="152"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="345" mass="38368" checksum="7FB3FE61E5970AC2" modified="2012-10-03" version="1">MEDYIGTRLLFETSKGMISGMLKAVDENVGKLVIEGEDGTRDVLIEDIQRLEIVEEPSSNISVESKAKEDEKAEVLNPSIGTSDKSTVEASRLHSVSHVGLRTGTGLMASSERYKSSQTDTSRTIYENGDNTRSSGKTGIEGKDDRNALETSEENYYGMMKRAFCYFGPLEEEFCSIAARQAYRIFSTYFGNSTLKVEVLVSGDGVFPSIGLILGRLLLNSGKKLSVICNEIPLRSVKYKQSYMNSGGIIANKPSLESTIQIYSCGKALISTWPRSSAKGFMYLDTPGFIVEERETKIGLCFGSIPTYFRKFNGIVYFVDMEYPSLLYEEFGLQRPTKSKIHKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7APF6</accession>
    <name>I7APF6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Chromatin assembly factor 1 subunit A dimerization domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265211.1">
        <property type="nucleotide sequence ID" value="XM_009266936.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APF6"/>
    <dbReference type="GeneID" id="20564322"/>
    <dbReference type="KEGG" id="ero:EROM_090980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090980"/>
    <dbReference type="HOGENOM" id="CLU_773936_0_0_1"/>
    <dbReference type="OrthoDB" id="2193302at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022043">
        <property type="entry name" value="CAF1A_DD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12253">
        <property type="entry name" value="CAF1A_dimeriz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chromatin assembly factor 1 subunit A dimerization" evidence="2">
        <location>
            <begin position="193"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="358" mass="41763" checksum="CBC1513047D137C8" modified="2012-10-03" version="1">MEVLFHPTVLRILHEWKKSQEEEIPVDLLALMVDCKLDVDYDWMDRVLYSYKKTSKRRKDRIKTLKEFVESKFKIEMINEKDKATRGVYLLKDLALVSGCVRKLLKGIRKDFKRSRPDGALDSSTNIRKKEVTTPGILKFVQIEGKKKSSRNTLGQFSPISFGDEVIISTLASPINSTVRIRKDLQNPKRLSFIKFSDQLKPPFYGFREEKLHVRNSLLKLPQVLDYEHDSDWEDAEDAETIGTTEEESEEEEGSYEWIELDSERREMSRLTKKPSLSFPSFKLNISSLFSPSWASLPLVDREVFPEELSQELIKELPFQKDLEEFIRKFGNRYVIKQSAINDKVKTLELGDVIKKMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7APG0</accession>
    <name>I7APG0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_091030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265216.1">
        <property type="nucleotide sequence ID" value="XM_009266941.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APG0"/>
    <dbReference type="GeneID" id="20564327"/>
    <dbReference type="KEGG" id="ero:EROM_091030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091030"/>
    <dbReference type="HOGENOM" id="CLU_162874_0_0_1"/>
    <dbReference type="OrthoDB" id="2189070at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="116" mass="12990" checksum="5DC660BC5F2C6D11" modified="2012-10-03" version="1">MVRVKSRYVVVKLRPEGNRSVPSMDVLGSIIKSKVEIDYGRHVLSMVGNLGVVEYLPHSQIAVIKCDAPACKHVLFTIATIGEISGFKCSMSVLWVSGILKRAMRKILKYVKMEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7APK2</accession>
    <name>I7APK2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA polymerase II assessory factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_091730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDC73 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265286.1">
        <property type="nucleotide sequence ID" value="XM_009267011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APK2"/>
    <dbReference type="GeneID" id="20564397"/>
    <dbReference type="KEGG" id="ero:EROM_091730"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091730"/>
    <dbReference type="HOGENOM" id="CLU_1030950_0_0_1"/>
    <dbReference type="OrthoDB" id="2186602at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11990">
        <property type="entry name" value="RNA polymerase II accessory factor, Cdc73 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007852">
        <property type="entry name" value="Cdc73/Parafibromin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031336">
        <property type="entry name" value="CDC73_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038103">
        <property type="entry name" value="CDC73_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466">
        <property type="entry name" value="CDC73 DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12466:SF8">
        <property type="entry name" value="PARAFIBROMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05179">
        <property type="entry name" value="CDC73_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="Cell division control protein 73 C-terminal" evidence="5">
        <location>
            <begin position="116"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="256" mass="29394" checksum="72E169DB15135968" modified="2012-10-03" version="1">MDPDEITIGGKKVSPKQTIKFKGTETKEYTLEQVLFYLLNKDKKYTAYMTLCRESGIGKIYYTDQKIIVEEIESFKEATIEAKIDGPDFRYIGFRDYSYLDDLCKKEGESKPTVYYIVVPQSVSSPVNLSNIKEFFEEGKCSEGIQISEVEKVELNIDGYKFAAVDDVSRFTSEDWKRVVCIFLDGTKWQVSRWNIRDVGEIFNTIPTFYFIKRGAPGNFYIKNCNVIGVETHGGKVDKPTLSSIRKRIKDCILGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ10</accession>
    <name>I7AQ10_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="10">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_101490</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="10"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265461.1">
        <property type="nucleotide sequence ID" value="XM_009267186.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ10"/>
    <dbReference type="GeneID" id="20564579"/>
    <dbReference type="KEGG" id="ero:EROM_101490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101490"/>
    <dbReference type="HOGENOM" id="CLU_034585_1_2_1"/>
    <dbReference type="OrthoDB" id="17102at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000016">
        <property type="entry name" value="Putative choline-phosphate cytidylyltransferase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="11">
        <location>
            <begin position="46"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="278" mass="32823" checksum="B4220290A2C91825" modified="2012-10-03" version="1">MKKENNPEEYRMIAPTYPDRNAFEYEEMNSDVGVYKIPTNRPVRIYCDGVYDLFHYGHARSLKQAKNLFPDVYLLVGVTDDNITTRLKGNLVMNEKERAEGLIHCRYVDEVITSAPWELTSEFIQKHKIDFVAHDDIPYKGEGKEDIYKFVKDMGMFIPIRRTKGISTSGIITNIVRNYDIYVRRNLERGVSAKDLNISFLHMKRIKMSKKMNEMIKNVDIQREIDEIRKEIRIAMRYWERVSNEIISGFIENFCHGKTSSKFIDRLVEFVKLKRKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ24</accession>
    <name>I7AQ24_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RasGAP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_101640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265476.1">
        <property type="nucleotide sequence ID" value="XM_009267201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ24"/>
    <dbReference type="GeneID" id="20564594"/>
    <dbReference type="KEGG" id="ero:EROM_101640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101640"/>
    <dbReference type="HOGENOM" id="CLU_011104_0_0_1"/>
    <dbReference type="OrthoDB" id="775356at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21206">
        <property type="entry name" value="CH_IQGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.506.10">
        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000593">
        <property type="entry name" value="RasGAP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001936">
        <property type="entry name" value="RasGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149:SF14">
        <property type="entry name" value="CALPONIN-HOMOLOGY (CH) DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14149">
        <property type="entry name" value="RAS GTPASE-ACTIVATING PROTEIN WITH IQ MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00616">
        <property type="entry name" value="RasGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03836">
        <property type="entry name" value="RasGAP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143885">
        <property type="entry name" value="RGC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="2">
        <location>
            <begin position="43"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1024" mass="118823" checksum="B363831F3F95003A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQ74</accession>
    <name>I7AQ74_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pre-mRNA cleavage and polyadenylation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_110470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265526.1">
        <property type="nucleotide sequence ID" value="XM_009267251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQ74"/>
    <dbReference type="GeneID" id="20564646"/>
    <dbReference type="KEGG" id="ero:EROM_110470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110470"/>
    <dbReference type="HOGENOM" id="CLU_008572_0_0_1"/>
    <dbReference type="OrthoDB" id="6109at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058543">
        <property type="entry name" value="Beta-prop_RSE1/DDB1/CPSF1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23726">
        <property type="entry name" value="Beta-prop_RSE1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 second beta-propeller" evidence="2">
        <location>
            <begin position="460"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="1">
        <location>
            <begin position="810"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1156" mass="131369" checksum="347A21AF01F26711" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQD4</accession>
    <name>I7AQD4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nbp35-like nucleotide binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_111070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265586.1">
        <property type="nucleotide sequence ID" value="XM_009267311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQD4"/>
    <dbReference type="GeneID" id="20564706"/>
    <dbReference type="KEGG" id="ero:EROM_111070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111070"/>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_1"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN84089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="301" mass="32832" checksum="9DB870BEC8BC8693" modified="2012-10-03" version="1">MGENCPGVSSKDAGKAEECKGCPNVSYCSQPMQQDPDIKAIQENLGGIRIIVAIMSGKGGVGKSTITRNIAECISARGVSTCILDLDLSGPSIPRLTATDGMSMCETNGMMQPVEVNRFLKVVSAGYVQNSYGEGRMFSSRLKTNALKKLLGHCNYKGVDTLLLDTPPNVTDEHLGMVNFIKPRLGIIVTTPQKFAMQDVIRQIDFCRKAKIDVLGIVENMKKFICPSCNHPKSVFRSTGVESYCKSNGVPYLGSINLRQDIAKTSDMGNAVRDDAFNRICDLIISLRETSEVKTTDSFRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQF8</accession>
    <name>I7AQF8_ENCRO</name>
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        <submittedName>
            <fullName evidence="4">Polar tube protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265611.1">
        <property type="nucleotide sequence ID" value="XM_009267336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQF8"/>
    <dbReference type="SMR" id="I7AQF8"/>
    <dbReference type="GeneID" id="20564732"/>
    <dbReference type="KEGG" id="ero:EROM_111330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111330"/>
    <dbReference type="HOGENOM" id="CLU_257808_0_0_1"/>
    <dbReference type="OrthoDB" id="2190813at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707881" evidence="3">
        <location>
            <begin position="17"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1151"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="425"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1155"/>
            <end position="1175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1254" mass="136126" checksum="43D039A7645B508D" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQJ6</accession>
    <name>I7AQJ6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_021170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264089.1">
        <property type="nucleotide sequence ID" value="XM_009265814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQJ6"/>
    <dbReference type="GeneID" id="20520879"/>
    <dbReference type="KEGG" id="ero:EROM_021170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021170"/>
    <dbReference type="HOGENOM" id="CLU_877252_0_0_1"/>
    <dbReference type="OrthoDB" id="2191488at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="317" mass="36112" checksum="8DFAC374F91F3F4C" modified="2012-10-03" version="1">MIIRIEEKYASSSVREWFGCLKSDEDVLEEDEKVSNGLPEYRIEIKENGEGNDATISVGKLNIKHKVALDVLEDTLIEVVGSLLGYEQTILYGFTVKDSSGTKMKPVVPIFFKSTLGVQIPKRLGYLKSDNSGMPEVDEGMMIPFVSISPIIKSVLTSGTLRFSAENVKVLLAPYNGSMFMIPNTANIIPMVRGMRSREDMGVFLKLLRSFPFKFVETFIKAYQQPSSVTVNRELSNCFSEFQNNLTKLDIKCIYKIVSSAPVFLDARDKITERVEKFYVYNARGFKRKKGQYLKCREELYIDRCPIGSLKRYFRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQR6</accession>
    <name>I7AQR6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264139.1">
        <property type="nucleotide sequence ID" value="XM_009265864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQR6"/>
    <dbReference type="GeneID" id="20520930"/>
    <dbReference type="KEGG" id="ero:EROM_030220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030220"/>
    <dbReference type="HOGENOM" id="CLU_075696_2_0_1"/>
    <dbReference type="OrthoDB" id="10264538at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00499">
        <property type="entry name" value="Ribosomal_eL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001380">
        <property type="entry name" value="Ribosomal_eL13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11722:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01294">
        <property type="entry name" value="Ribosomal_L13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="163" mass="18932" checksum="08F66117A28DD3F9" modified="2012-10-03" version="1">MKGNHALPNNHFRKTSLKIKVHHDPETKLRIMEEKKLRKAKSMFPIPLKKLRPIIRCPSIKYNRNERLGRGFTPAECEKAGLDYRHARRLGIAVDLRRRDTNQETLEKNADRIKTYLSKITIYESVKEAKEKGAKPYAKEIMPLVKPKPVVGSISREELASYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQS4</accession>
    <name>I7AQS4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_030310</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN82652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264149.1">
        <property type="nucleotide sequence ID" value="XM_009265874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQS4"/>
    <dbReference type="GeneID" id="20520942"/>
    <dbReference type="KEGG" id="ero:EROM_030310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030310"/>
    <dbReference type="HOGENOM" id="CLU_004627_1_0_1"/>
    <dbReference type="OrthoDB" id="10251642at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.210">
        <property type="entry name" value="FACT complex subunit Spt16p/Cdc68p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056595">
        <property type="entry name" value="Fact-SPT16_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013953">
        <property type="entry name" value="FACT_SPT16_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24824">
        <property type="entry name" value="PH_SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08644">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01286">
        <property type="entry name" value="SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="12">
        <location>
            <begin position="6"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 middle" evidence="13">
        <location>
            <begin position="399"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="Histone chaperone RTT106/FACT complex subunit SPT16-like middle" evidence="14">
        <location>
            <begin position="650"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="781"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="783"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="798"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="808"/>
            <end position="846"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="854" mass="98589" checksum="FB30CE283D34CAA2" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQX6</accession>
    <name>I7AQX6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">V-ATPase proteolipid subunit C-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_031000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="20521013"/>
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        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
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        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="6">
        <location>
            <begin position="101"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="166" mass="17781" checksum="7518D97FA023D408" modified="2012-10-03" version="1">MFLLMGPIISMDAGNDSDTAFSIVSIGLAIEMALAVYGACEGMASSGGASILCSRNRSILGPTYFIMIMVSTIFFSSFLVALIIATNIDEKLDMAKSISYFSACLMVGITAAISGKVCSTLGRRGFRILSEKPSFLPILIILFGMIEFVIIVVLSCALLMVYQTRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR10</accession>
    <name>I7AR10_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_040060</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR10"/>
    <dbReference type="GeneID" id="20521070"/>
    <dbReference type="KEGG" id="ero:EROM_040060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040060"/>
    <dbReference type="HOGENOM" id="CLU_614068_0_0_1"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051787">
        <property type="term" value="F:misfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051727">
        <property type="entry name" value="DnaJ_C3_Co-chaperones"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44140:SF2">
        <property type="entry name" value="LD25575P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="374"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="492" mass="57663" checksum="0D111067D864E239" modified="2012-10-03" version="1">MTAQDTLKREEKFLGLFRRICVGNKVVFKAVIGLFLGIVRTQLDLANEVFKADISVKNGDLLKAESIYKEISQYFRDNDVFRKKYAEFLMAKGEYGEVIKGFGKDPEMKEKVKRANECLSILVSNNIRLIATLIKESPYSKLVLEAYIQVCLVDEKLEEAEKFVKKSRSLFPNEPGFAHQEMQLYFLRGMFSPGIKALNSLEYKEMASSFASILESYEWIKRSNLNSKDKCKRLDKLVRTISLIESDSNFFPSIFSTLKLSVIFDLCKSGVEGGVQGLSSRARYLYSKRNNEDTMYLYIMALILDGNIKEAEEKFNLFKFRNMKLKNHISHRLQMVKDEREKKKREKEEKKYRRRGYTEENYGGMPRNKGDFLGYYKTLGFKEGERPDEKEIKKAYRKLVVENKKKKKNPQEEKEWEENFKKLNKAFGVLRDKKKREMYDKGIDPDNPQQAAAGFGNDPFQEIFRGFGNFGFFDFPDQRRGRRRSTTTYFYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARI1</accession>
    <name>I7ARI1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Synaptobrevin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264479.1">
        <property type="nucleotide sequence ID" value="XM_009266204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARI1"/>
    <dbReference type="GeneID" id="20521281"/>
    <dbReference type="KEGG" id="ero:EROM_050490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050490"/>
    <dbReference type="HOGENOM" id="CLU_184655_0_0_1"/>
    <dbReference type="OrthoDB" id="2192234at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="2">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="91" mass="10407" checksum="08CE747A1761A53E" modified="2012-10-03" version="1">MGDEVTQKIEKEVEDLQGALKEQLKAQRDLGDLETNLKSETFTLEKTTEEHKAATEEAKNKLFWKNFKYFLIAGILIILVLFGIFGNIFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARU4</accession>
    <name>I7ARU4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264599.1">
        <property type="nucleotide sequence ID" value="XM_009266324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARU4"/>
    <dbReference type="GeneID" id="20521404"/>
    <dbReference type="KEGG" id="ero:EROM_060070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060070"/>
    <dbReference type="HOGENOM" id="CLU_630090_0_0_1"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="447" mass="52406" checksum="9CFECC9E9453BED2" modified="2012-10-03" version="1">MNSLKEYVKNIDFEVVDDSEILDNVYKEIQGNEKELMLDTETTHQIETLIRKSNSRQILDFFKKLDVERLMSKKLGSRVIEVIYDAVFDMVYIRRQNIDIDMLVKPAEDVLVTKFSDTNATHIIRKLFQLVSGKRILDDKIQSFNSVRPGHIEKYKEIITGLIPNLSKEDSFITLLTYLYCCRSKSIVYEVVKHHFTWIKVCDPSMSYFFEKIVRLAGRKAKKYIFENIKDKVMELCKEKYGNYFIGEFILWYHQKADYFFEAIDLSEFDKNSNIVMKLTHSLLKARSYPNIDKIVKGFYLENGNDIISCTFGGVEGNFKQKYAPMLCELMKLPNENNYGINVAFRRNFSSRWIRSKGGIELLRGYYEGVDDGRSKRNFTKQIEEHLPLIASRTENNIILEFMRMYGSQKAGIEIRKRHQSAKKDISTKPKEYFQSTKPAPRYVGTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS41</accession>
    <name>I7AS41_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_061100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264699.1">
        <property type="nucleotide sequence ID" value="XM_009266424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS41"/>
    <dbReference type="GeneID" id="20521506"/>
    <dbReference type="KEGG" id="ero:EROM_061100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061100"/>
    <dbReference type="HOGENOM" id="CLU_142390_0_0_1"/>
    <dbReference type="OrthoDB" id="2188412at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF137">
        <property type="entry name" value="INO80 COMPLEX SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="35"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="145" mass="17296" checksum="A0D132D55C5586DC" modified="2012-10-03" version="1">MKCEWEGCQKDIEDNVRDHLLSHIEKNDEAKCLWRGCQRYGEAQVSKHALLAHARRHTGERPFECHLCGKDYTRSDPLKKHLLRHEVADSKNENLMMKIEYLGLLLTEYRRESLRIMNDIESIRYNIQTMNRKIAYEIKRNKSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS64</accession>
    <name>I7AS64_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">U3 small nucleolar RNA-associated protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_070010</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264749.1">
        <property type="nucleotide sequence ID" value="XM_009266474.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS64"/>
    <dbReference type="GeneID" id="20521559"/>
    <dbReference type="KEGG" id="ero:EROM_070010"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070010"/>
    <dbReference type="HOGENOM" id="CLU_084032_0_0_1"/>
    <dbReference type="OrthoDB" id="28112at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013949">
        <property type="entry name" value="Utp6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055347">
        <property type="entry name" value="UTP6_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271">
        <property type="entry name" value="HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN 66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23271:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08640">
        <property type="entry name" value="U3_assoc_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 6 N-terminal" evidence="5">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="256" mass="30666" checksum="8FCB9AC2BBBBBF7D" modified="2012-10-03" version="1">MVESMQLGMERMISELEDYKNRGIFSSDELRKIVETRRKYELRLQRPEKKLLDFVRYIKSECMLEKIRDKRLREKNVNASLHDMIIPKKVVELYRSALYRFNDPKIISQFTAYVIEKKMYGEMKNVFAECCTRNPMDVDLWIYCGFKLFEVGDIESSRAMFLKGIRMNGKNTRIRIEFFRMEVMYVEKIEALNKEIGLDEEDRDEIERGEVAFAIFSDCLNDSSFTEKEIIEVLEISKSIKELRERIEEYIKDKEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS78</accession>
    <name>I7AS78_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_070210</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264769.1">
        <property type="nucleotide sequence ID" value="XM_009266494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS78"/>
    <dbReference type="GeneID" id="20521580"/>
    <dbReference type="KEGG" id="ero:EROM_070210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070210"/>
    <dbReference type="HOGENOM" id="CLU_085115_0_0_1"/>
    <dbReference type="OrthoDB" id="2190402at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061685">
        <property type="term" value="F:diphthine methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052415">
        <property type="entry name" value="Diphthine_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46042:SF1">
        <property type="entry name" value="DIPHTHINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00043952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="279" mass="31557" checksum="99650A0285FA34A2" modified="2012-10-03" version="1">MEAFEADIPIDCLCCTSSALVAGGYLFNEGVRSGKIYFYSLESMNIEDEFTTSGTLDIKVSEQTLYSANSSDISAIHLGSRSTIKMETAHINTYVEVYGSNVFVFDVGGRVNVYSKEMTSAKVIKAGDAPIWALKASDKELICGSEDGMLRFVDTRTFSEHHTMKRTSGITSMYEYLEYLYVGSYDECIEIIDKRKYEIIKKVRVGGGIWRICKEGEMFYLSCMYEGLKVCDCDLNVMEKFPTNSIAYGLTVKDSKVFFTSFYDKKIYKFERHVLGICE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASB4</accession>
    <name>I7ASB4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264814.1">
        <property type="nucleotide sequence ID" value="XM_009266539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASB4"/>
    <dbReference type="GeneID" id="20521625"/>
    <dbReference type="KEGG" id="ero:EROM_070660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070660"/>
    <dbReference type="HOGENOM" id="CLU_1570637_0_0_1"/>
    <dbReference type="OrthoDB" id="2191313at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="170" mass="19212" checksum="053B52968536ABA4" modified="2012-10-03" version="1">MRLKALVRKRANDLLLSLRSLRAEICREDVFGQILGNIHKSYIRLVTLLSKPKVQRQELTEIDSKNGIVKYKAGELEFLYHAEHGIISVSGGDIGIDNHILCSIRSEPTDKHLGLANDMLMMYIGYERAPCDVCGSYATIPGFLTPTCRSIEEDFILVHHSQCKVEQLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASU1</accession>
    <name>I7ASU1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rad21/Rec8-like protein C-terminal eukaryotic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080970</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265009.1">
        <property type="nucleotide sequence ID" value="XM_009266734.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASU1"/>
    <dbReference type="GeneID" id="20521831"/>
    <dbReference type="KEGG" id="ero:EROM_080970"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080970"/>
    <dbReference type="HOGENOM" id="CLU_930746_0_0_1"/>
    <dbReference type="OrthoDB" id="2195257at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="1">
        <location>
            <begin position="263"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="300" mass="34311" checksum="6F21D6579AFB9F56" modified="2012-10-03" version="1">MRRMRVVLKFSFEDKEMSSLFSVLINEGKKIGRKKGPIARVRISKVVEIIMNSVPVYNLRISSVLFRVIVRAYEAKVRVYLLEIQSLVKILSITKRRGEYKKKKGRNMLSMDMGHISSICSSEFDDSQYMDGQIGSYSETLGLVDGYTDQPKRRSKGITDRMTELDIPRMIVSDTSRNAPVISSFEPVRKEPFVVREARKIKPALQNIEIEYSEMSTLGSIERPRNASVHTVRESSSSSVSEERTLDFLSSDTDFNKYAVGGRRRRARIFYLMLELSSRGAFVPVQKQAYGEIVLTKPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASX2</accession>
    <name>I7ASX2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="8">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_081310</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="8"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265044.1">
        <property type="nucleotide sequence ID" value="XM_009266769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASX2"/>
    <dbReference type="GeneID" id="20521866"/>
    <dbReference type="KEGG" id="ero:EROM_081310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081310"/>
    <dbReference type="HOGENOM" id="CLU_028946_3_0_1"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="9">
        <location>
            <begin position="3"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="319" mass="35753" checksum="0FFF5A717D0F83E4" modified="2012-10-03" version="1">MELKVDEHYSFIQRTIHTKNFLYYLTEPYRLNTIYWSVNSLSMLGKEEIEEMKGRVIDYVMRCKNDDDGGFGGCEGYSSNITSTFNALQILYIYRIHYRDRSTASFISKLLQPEGYFFNDSYGEVDTRINCCGVLGLHLLSLLEKGDFDPKSLSSPICKVFLSEVGIDIKTIVSYTQKCYNLDGGFGAVEGAESHAAQVFCCLSTLRSLGALELIDVEGVTRFVAMKQMVSGGLSGRVSKKEDVCYSFWAYSSLVLIGRESRVNQKELVKFILACQGRYGGFSDRPGNEADLYHLMFALAGLSLLGYKGLKKIDPGFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>I7ASY5</accession>
    <name>I7ASY5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081460</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265059.1">
        <property type="nucleotide sequence ID" value="XM_009266784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASY5"/>
    <dbReference type="GeneID" id="20521881"/>
    <dbReference type="KEGG" id="ero:EROM_081460"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081460"/>
    <dbReference type="HOGENOM" id="CLU_1768034_0_0_1"/>
    <dbReference type="OrthoDB" id="2190021at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="161" mass="19474" checksum="B4E693C2549D4756" modified="2012-10-03" version="1">MGFLYWVGKQALHRMSWKFLNVLKERTGMESITKEQIKGIRELVKKAQEWDEKKAQRHVMFSSKMSDEEYTNLWKVDSYIIDGNDKEKLKNLDQRTVFNNIMWLSRNVEKIDSNMKKFLGMHVWVVNYWIKTVGSATVRKFLRENNYSGEFVYFLYKYFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT70</accession>
    <name>I7AT70_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090090</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265124.1">
        <property type="nucleotide sequence ID" value="XM_009266849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT70"/>
    <dbReference type="GeneID" id="20521948"/>
    <dbReference type="KEGG" id="ero:EROM_090090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090090"/>
    <dbReference type="HOGENOM" id="CLU_1579007_0_0_1"/>
    <dbReference type="OrthoDB" id="2194038at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="171" mass="19802" checksum="68E6986EE12660F2" modified="2012-10-03" version="1">MGLSFPSGHVEGLKDSLRKRVEEKKRSAEIYKDFVKRMYELENELDFISAEDQKSMVESTKRRRRSTTLDVDEILYTAVDETAKDLRRILGGEKDLDVFIENNALNIKGCVFTKGDKIKARIKREEFVGAIVSIGEEDIVLKTKEYKRLKILLDDIRSTKSSIMPINEIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATG3</accession>
    <name>I7ATG3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FusA-like translation elongation factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091210</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265234.1">
        <property type="nucleotide sequence ID" value="XM_009266959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATG3"/>
    <dbReference type="GeneID" id="20564345"/>
    <dbReference type="KEGG" id="ero:EROM_091210"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091210"/>
    <dbReference type="HOGENOM" id="CLU_439421_0_0_1"/>
    <dbReference type="OrthoDB" id="2193278at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF6">
        <property type="entry name" value="116 KDA U5 SMALL NUCLEAR RIBONUCLEOPROTEIN COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="1">
        <location>
            <begin position="2"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Elongation factor EFG" evidence="2">
        <location>
            <begin position="401"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="505" mass="57103" checksum="F8BF190EDC4159AD" modified="2012-10-03" version="1">MTLLDTPGHSDLIHETTKAIECIDIAVVVLDICCDLSLQYEVVFNAIAASKKPLIIVLNKTDLIGRDFASFIKKKIEHLRSIVEEKMCPRGWFAVSSKYGSLSRIKADVYESNEKSEDVLNELIEEIFCLEPTKHNYNDEERFGGFLWAGGTILFGLVPKADFNAGSTIKVYGTDLAAEKLYIPFPGCLVETRAVKANIGVLVKFQEGLLQKIELPNNQKVLGHTIKKVLEDKGLMCTDSELLEVVDPVIRVQVSPSDEDIFLREVWKLRLIYPGLKIGKKSLCGNGELLMDAVLYDLRNKLNVEFQVLSVSSNLKEIFKDSFREVVETGKGLLTVEGGVTRDIDEDHKFSGYIEKQILSKGPLIKEPFSHSYLAISPEPLELDFECFRKIEDSIVKHVTILEPLYLVEIVYTSEAEDLVNEMIKSSFGEVIRQKSFPFSMLKSILCYIPVPESFGFETDLRVYSCSKADCIKIPLYWRPVSDKSRAASLTRFMRKTKRFELHGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATG7</accession>
    <name>I7ATG7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NEDD8-activating enzyme E1 catalytic subunit</fullName>
            <ecNumber evidence="1">6.2.1.64</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_091260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalytic subunit of the dimeric E1 enzyme, which activates NEDD8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="6.2.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.64" evidence="1"/>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265239.1">
        <property type="nucleotide sequence ID" value="XM_009266964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATG7"/>
    <dbReference type="GeneID" id="20564350"/>
    <dbReference type="KEGG" id="ero:EROM_091260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091260"/>
    <dbReference type="HOGENOM" id="CLU_013325_13_0_1"/>
    <dbReference type="OrthoDB" id="10255449at2759"/>
    <dbReference type="UniPathway" id="UPA00885"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019781">
        <property type="term" value="F:NEDD8 activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953:SF6">
        <property type="entry name" value="NEDD8-ACTIVATING ENZYME E1 CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="UBIQUITIN-ACTIVATING ENZYME E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="2">
        <location>
            <begin position="2"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="275" mass="31281" checksum="6BAB346BDC6F3A25" modified="2012-10-03" version="1">MKMILVGCGGTGVELLKVLKDMASEVLVIDHDRINLTNLHRLFMFTRQDKGEYKSQKAAEFVSNLYGIKASFLTQRIEEVSVDVFDRYDVIFGALDNIEGRMNLNLMFRRSRCKLLIDCGISGYKAHAKAVYKESSCLYCIRELYREDGVGMCSLGSLPKEITLENREKVLRSLVELKRDGLGSKREKIEQIVNEFNALAKEKLRTDVFEVTGMYDGIIPNVCFINSVCASLACRLLDGPNLSYDFIFYSGEENMVFEKLHLAKDEKCIVCRMDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATL6</accession>
    <name>I7ATL6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265309.1">
        <property type="nucleotide sequence ID" value="XM_009267034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATL6"/>
    <dbReference type="GeneID" id="20564422"/>
    <dbReference type="KEGG" id="ero:EROM_091980"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091980"/>
    <dbReference type="HOGENOM" id="CLU_1294390_0_0_1"/>
    <dbReference type="OrthoDB" id="2193324at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="213" mass="24801" checksum="E2B1A818EB42C522" modified="2012-10-03" version="1">MIISWTDAFKVLRDTKVKERLLRNNLTFEYLARQLSTMNCSLCYKPISIKGLLCEGCFYKLHPPSIDFKTSIAVKYALELQRIRINRTSSLKPSTKDESVSFKLLDISNDSSLRDLSGKVYSLESASLSYEERIILSSIKLKIQMETTNKWAAIRIKSMIDLIDEQLNILRLKYLICLRNLRLDDVLLLKKHIIEIRKHRDSLFEMLGNKLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU23</accession>
    <name>I7AU23_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucyl aminopeptidase</fullName>
            <ecNumber evidence="3">3.4.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_101670</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.</text>
            <dbReference type="EC" id="3.4.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M17 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.1" evidence="3"/>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265479.1">
        <property type="nucleotide sequence ID" value="XM_009267204.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU23"/>
    <dbReference type="GeneID" id="20564597"/>
    <dbReference type="KEGG" id="ero:EROM_101670"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101670"/>
    <dbReference type="HOGENOM" id="CLU_013734_6_3_1"/>
    <dbReference type="OrthoDB" id="412814at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00433">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="entry name" value="Leucine Aminopeptidase, subunit E, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00181">
        <property type="entry name" value="Cytosol_peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011356">
        <property type="entry name" value="Leucine_aapep/pepB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000819">
        <property type="entry name" value="Peptidase_M17_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023042">
        <property type="entry name" value="Peptidase_M17_leu_NH2_pept"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963:SF23">
        <property type="entry name" value="CYTOSOL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11963">
        <property type="entry name" value="LEUCINE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00883">
        <property type="entry name" value="Peptidase_M17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00481">
        <property type="entry name" value="LAMNOPPTDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="Macro domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00631">
        <property type="entry name" value="CYTOSOL_AP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707436" description="leucyl aminopeptidase" evidence="7">
        <location>
            <begin position="19"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Cytosol aminopeptidase" evidence="8">
        <location>
            <begin position="345"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="502" mass="54400" checksum="168836825C95C195" modified="2012-10-03" version="1">MGLNLKLLINFLLVPIRMELMNYEKLVVMSEPSTDSTKVRIVLCSNTEEGVSIISDEKDSSHREFLNSLGAKGNLGENYILPERNGRITVFVGIGKVDENILLVKSNARKAGASGYKAVSQFRNIEISLGSEFMAREVVSGIILASYKYQFLNKEEGESKRMAINSQVDAVKKAITIGNAQNFARFLGDTPANLMNPTLFTEYATKYLQGKRNVTVEVFGKDFMKSKSMNLLLSVSQGSSQEPKLLVAKYRGKSGEGVDLALVGKGVCFDSGGISLKPSARMCRMKGDMLGGASVLSAFGVIADMGVKINVDLVIPLVENLPSGTATKPGDVYIGMNGKSVEIDNTDAEGRLILADALVYAQERNPTYVFDVATLTGAMSIALGDVFIGYFTEDDNLSEMIYRSGIDANDPTWRMPLSQLYLPAMKSDVADLKNTGEGRYGGSSSAAIFLNEFVKKECKWVHFDIAGVMGSNNNKGVYGDGMTGCSVPVLVEIIERLSGVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU39</accession>
    <name>I7AU39_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN83997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265494.1">
        <property type="nucleotide sequence ID" value="XM_009267219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU39"/>
    <dbReference type="GeneID" id="20564612"/>
    <dbReference type="KEGG" id="ero:EROM_110130"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110130"/>
    <dbReference type="HOGENOM" id="CLU_404945_0_0_1"/>
    <dbReference type="OrthoDB" id="2196329at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="699" mass="79267" checksum="58E2416CBDB51D65" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUL9</accession>
    <name>I7AUL9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_111810</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265659.1">
        <property type="nucleotide sequence ID" value="XM_009267384.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUL9"/>
    <dbReference type="GeneID" id="20564780"/>
    <dbReference type="KEGG" id="ero:EROM_111810"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111810"/>
    <dbReference type="HOGENOM" id="CLU_051898_0_0_1"/>
    <dbReference type="OrthoDB" id="1716531at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="233"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="243"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="326"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN84162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="343" mass="39071" checksum="2CEC18FA8B9D62E6" modified="2012-10-03" version="1">MNNEFLLGQILKVPPITRYMILLISTVALLVYIDAVSPYSLYYSPLFLKKLEIWRVFTSFLYFGRPTLDMFMHVVFLYRYSRMLEEGCVSTSEYFWLIFVISSALFVISNIYGISALGTSFSSTITYIWTKRNPRAIVQIFGFISFPAFYLPFILPGFMLLSKRSISIDDVLGIVVGHLFHYFKDVYPRWGRDILRTPCWAKKLFNEHPPGCCRAQKGITIGEGRAKYEKNIGSSTRNDENASTNSVNVTNSKNYAKDDEDVNVEAPSQSCVESSKDLCKVSSLAPISVSTVMAPSEVPENNCFDLTDTLCNEEIVTENIPKEKESDEDWDEKWGESDNESTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGQ1</accession>
    <name>I6ZGQ1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein PNS1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_010180</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probably involved in transport through the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CTL (choline transporter-like) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263860.1">
        <property type="nucleotide sequence ID" value="XM_009265585.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGQ1"/>
    <dbReference type="GeneID" id="20520644"/>
    <dbReference type="KEGG" id="ero:EROM_010180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010180"/>
    <dbReference type="HOGENOM" id="CLU_056395_0_0_1"/>
    <dbReference type="OrthoDB" id="44736at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007603">
        <property type="entry name" value="Choline_transptr-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12385:SF88">
        <property type="entry name" value="CHOLINE TRANSPORTER-LIKE PROTEIN CTL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04515">
        <property type="entry name" value="Choline_transpo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="136"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="408" mass="45611" checksum="2DA289B80EC54FF5" modified="2012-10-03" version="1">MEETKENSRKFHDAWAFILYTILTALTTSWMVSEMKSLPQYNEEALLPLLTVNVGIMLIFLLAAYLGFRYAAKGFIFFANAVAPGILFFLSFLTLNPFAILSAGITFGISLVFYFWSIKPILPLMGPMIEATAKILSLNLIGCTILLLASVSLQVIQSFIFLKVLGNDDAGKTIMSVLFILNSYWTFFNTIYFAQVVVSGIVTTHVKNEKKGSFWESFYIAFMALGSISFAGLIMAAVSTAKFLVERERNRNRERGSSNIINIIILCILSIVGDLVNLMNELVFPYLAVHGTKYTESVSKSYNLIEEKGGIILLSNIAIEKVIFFCLLFFIYFIASGTTALLLRNPSYQNDLTYFTVGIVCAPLITFVYSFLSMFSSGTLALIYSYLESPEMVKKVEPELIEKISSFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGR1</accession>
    <name>I6ZGR1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Mediator of RNA polymerase II transcription subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Mediator complex subunit 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MED6</name>
        <name evidence="9" type="ordered locus">EROM_010280</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263870.1">
        <property type="nucleotide sequence ID" value="XM_009265595.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGR1"/>
    <dbReference type="GeneID" id="20520655"/>
    <dbReference type="KEGG" id="ero:EROM_010280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010280"/>
    <dbReference type="HOGENOM" id="CLU_077754_5_0_1"/>
    <dbReference type="OrthoDB" id="344220at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.580">
        <property type="entry name" value="Mediator complex, subunit Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007018">
        <property type="entry name" value="Mediator_Med6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038566">
        <property type="entry name" value="Mediator_Med6_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13104">
        <property type="entry name" value="MED-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04934">
        <property type="entry name" value="Med6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="177" mass="21108" checksum="549BFE9C855D889C" modified="2012-10-03" version="1">MEEKEESISFFDQRFLSSKSLDTTNILEYFSGSPFYDKSCNNEILKMQTQFRGLDQKSKLSSMIGIFYEVESSNNERTLFVIRKAYNHGDTTETLGMYYVIHGHVYAAPTNYSIYRCRMGDSMWLLNSFIEKMMEKRRFNPFNLSKRKHFRKGLEDNKDLSFMMEIFNDFKKEQMEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZGX2</accession>
    <name>I6ZGX2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RNA-splicing ligase RtcB homolog</fullName>
            <ecNumber evidence="10">6.5.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3'-phosphate/5'-hydroxy nucleic acid ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_010940</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10 13">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Catalytic component of the tRNA-splicing ligase complex.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="10">Ligation probably proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RTCB reacts with GTP to form a covalent RTCB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.8" evidence="10"/>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263935.1">
        <property type="nucleotide sequence ID" value="XM_009265660.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZGX2"/>
    <dbReference type="GeneID" id="20520721"/>
    <dbReference type="KEGG" id="ero:EROM_010940"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010940"/>
    <dbReference type="HOGENOM" id="CLU_022279_0_0_1"/>
    <dbReference type="OrthoDB" id="10249697at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072669">
        <property type="term" value="C:tRNA-splicing ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03144">
        <property type="entry name" value="RtcB_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027513">
        <property type="entry name" value="RtcB_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 10 13" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0819">tRNA processing</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="10 11">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="209"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="325"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="374"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <begin position="400"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 12">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN82438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="477" mass="52275" checksum="E3F5E55E346DC532" modified="2012-10-03" version="1">MKDVEQAGLCIKINPSFNKSMRTSGTIYVDEELRKPLIEELNAGVTPSSIGQISDVCSLPGLIGNVIGLPDIHTGYGFPIGCVAAFDINNPESVVSPGGIGYDINCGVRALRTNLDISCFKEKAESLADELFGIIPTGMGSDKRSNVDLGALDSILDQGLDYLLKLKEIPDEDAEYCESNGRMTGDSRLVGQKAKGRGLNQLGTLGSGNHYLEVQYVDEVYDTEKAEVMGIRKKQIVLMIHSGSRGLGHRICHDSLMRMARMSTGYGQLACSPYNSKESQEYLLSMGCAANFAFVNRAMITKKARQAFERVFPEARLDLIYDVCHNIAKIESHKINGMEYDAIVHRKGASRAFPPGHRDIPWKYKEMGQPVVVGGSMGTYSYILCGSEKSMELSFGSSCHGAGRVLSRGKSKDLFTHKNVCDLLRDQDIVFRCPSDPGMVEESPGCYKDINRVVDLSDRIGLTKKVCRLKPCLVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH06</accession>
    <name>I6ZH06_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminopeptidase N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_011290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263970.1">
        <property type="nucleotide sequence ID" value="XM_009265695.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH06"/>
    <dbReference type="GeneID" id="20520756"/>
    <dbReference type="KEGG" id="ero:EROM_011290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011290"/>
    <dbReference type="HOGENOM" id="CLU_110389_0_0_1"/>
    <dbReference type="OrthoDB" id="10031169at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="1">
        <location>
            <begin position="36"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="217" mass="24209" checksum="1F2A35F2D15179D0" modified="2012-10-03" version="1">MRWVELMIGMIMPMIESKNMEDKNGSGPLKLSRVVVPSHYDLHVKVMDAGFCGSVGIQVDISEPVDEIVMNAKELEIWDAGIVVGDKRIDGEVVVGDYEKEYEVVRLRFPSRIPKGEGYIVAEFCGDYNSGLVGFYKSGGDSKIYSTHFEPTDARRVFPCFDQPDMKATFSISLDAPANLVVLSNSPAVGSSRKEYGDRAIEYFEKTSKMSTYLLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH48</accession>
    <name>I6ZH48_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">T complex protein 1 delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_020430</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the T-complex protein 1 (TCP1) complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264015.1">
        <property type="nucleotide sequence ID" value="XM_009265740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH48"/>
    <dbReference type="GeneID" id="20520801"/>
    <dbReference type="KEGG" id="ero:EROM_020430"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020430"/>
    <dbReference type="HOGENOM" id="CLU_008891_9_2_1"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="332" mass="36521" checksum="273349C57C0C6CC1" modified="2012-10-03" version="1">MAIAAIKGMEHAKGDKRKIRVVKKIGGGLDDVKAYKSILLECDLKDIPKKVKLGVIQFCLSAPKTNMDSKILINDSALMEKLIQEERRYILEMCKKIKKSGCTLLVIQKSILRESLSDLASHFLKQLNISVVNSVDRKDVDYICSTMNVQPVSEIDLLNSGSLVDVETGEVEGMLEIKGYGCTILVRGCDDMVVEEAERSLNDALCVVKCLEELPFLVPGGGSIEMGIALMLSESTEGNVYVLREIAKAFEGVPYFLARNAGLYPVEIVSELRNELKQNDYVGISVRSGHAGDMVREDSVVQPAKVSINVITLAIETVSMILKIDDILPARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH63</accession>
    <name>I6ZH63_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Muskelin N-terminus domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_020580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264030.1">
        <property type="nucleotide sequence ID" value="XM_009265755.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH63"/>
    <dbReference type="GeneID" id="20520818"/>
    <dbReference type="KEGG" id="ero:EROM_020580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020580"/>
    <dbReference type="HOGENOM" id="CLU_004210_1_0_1"/>
    <dbReference type="OrthoDB" id="2188291at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052456">
        <property type="entry name" value="CTLH_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010565">
        <property type="entry name" value="Muskelin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15526">
        <property type="entry name" value="MUSKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15526:SF5">
        <property type="entry name" value="MUSKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06588">
        <property type="entry name" value="Muskelin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Muskelin N-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="734" mass="85136" checksum="7DA448F21A0CF09A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZH79</accession>
    <name>I6ZH79_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264050.1">
        <property type="nucleotide sequence ID" value="XM_009265775.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZH79"/>
    <dbReference type="GeneID" id="20520838"/>
    <dbReference type="KEGG" id="ero:EROM_020780"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020780"/>
    <dbReference type="HOGENOM" id="CLU_434758_0_0_1"/>
    <dbReference type="OrthoDB" id="2190823at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="626" mass="71981" checksum="7986EEB3C20C5FB4" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHD0</accession>
    <name>I6ZHD0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_021330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264105.1">
        <property type="nucleotide sequence ID" value="XM_009265830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZHD0"/>
    <dbReference type="GeneID" id="20520895"/>
    <dbReference type="KEGG" id="ero:EROM_021330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021330"/>
    <dbReference type="HOGENOM" id="CLU_1434429_0_0_1"/>
    <dbReference type="OrthoDB" id="2190718at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="189" mass="21086" checksum="D322EC43683A5011" modified="2012-10-03" version="1">MEIKTGLVGKALSSCYLKTNKTSAICCIHFEQKGETDPWVEVNFSEMIPLEIRSPISSIANSLLSSKARSTKIKVSFHTLRHGEDLLSCLINSFSICVILSGIRVVDTICSSTVYAGKDRIALTEGPDLHSLHMVYMIHRKRIMQVHFSGEMDFSLLAEATEKLVHRCTEQGELIRSKVEGVAKDMVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZHJ5</accession>
    <name>I6ZHJ5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_030190</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264135.1">
        <property type="nucleotide sequence ID" value="XM_009265860.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20520926"/>
    <dbReference type="KEGG" id="ero:EROM_030190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030190"/>
    <dbReference type="HOGENOM" id="CLU_000487_3_0_1"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02584">
        <property type="entry name" value="RNAP_II_Rpb1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02733">
        <property type="entry name" value="RNAP_II_RPB1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.30:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.390:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.120:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007075">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007073">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038593">
        <property type="entry name" value="RNA_pol_Rpb1_7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF37">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04992">
        <property type="entry name" value="RNA_pol_Rpb1_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04990">
        <property type="entry name" value="RNA_pol_Rpb1_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00115">
        <property type="entry name" value="RNA_POL_II_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15 18" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="17">
        <location>
            <begin position="203"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1462"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1467"/>
            <end position="1492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1499"/>
            <end position="1515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN82638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1515" mass="169389" checksum="B1E6593D30F3E777" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI36</accession>
    <name>I6ZI36_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ham1 nucleoside triphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_041170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264380.1">
        <property type="nucleotide sequence ID" value="XM_009266105.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI36"/>
    <dbReference type="GeneID" id="20521180"/>
    <dbReference type="KEGG" id="ero:EROM_041170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041170"/>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_1"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="192" mass="21353" checksum="88E30D18E70CBF2C" modified="2012-10-03" version="1">MGRIYFATTNLKKLKEVRRPFNTDIVHMNVSMVEIQASQERVVHNKLDQVMALISKEDAVIVDDTGVFLDSLCGFPGVYIKDFLKIGSQKILEILKKVEDNNATVSYTLGIAHYKDGQIAKKAFSGEIKGTIMESKEEGSAELDGIFIPDEFKASLKSMSIDEKNCISHRRIATENLTDYMVSIGIIKASSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI79</accession>
    <name>I6ZI79_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription initiation factor 4F subunit-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_041640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264425.1">
        <property type="nucleotide sequence ID" value="XM_009266150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI79"/>
    <dbReference type="GeneID" id="20521227"/>
    <dbReference type="KEGG" id="ero:EROM_041640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041640"/>
    <dbReference type="HOGENOM" id="CLU_446896_0_0_1"/>
    <dbReference type="OrthoDB" id="514777at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23253:SF78">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4G1, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIF4G" evidence="1">
        <location>
            <begin position="187"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="609" mass="70936" checksum="4D1F96412BCD798A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIA5</accession>
    <name>I6ZIA5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADPH cytochrome P450 reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_050200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264450.1">
        <property type="nucleotide sequence ID" value="XM_009266175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIA5"/>
    <dbReference type="GeneID" id="20521252"/>
    <dbReference type="KEGG" id="ero:EROM_050200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050200"/>
    <dbReference type="HOGENOM" id="CLU_001570_17_6_1"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF10">
        <property type="entry name" value="NADPH-DEPENDENT DIFLAVIN OXIDOREDUCTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="3">
        <location>
            <begin position="182"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="509" mass="59156" checksum="654795B911184848" modified="2012-10-03" version="1">MVPILYGSQTGTSIYVSKLIERAILYGYDARTIYNLESFSSSQDQRSMSIVVEMDLFDIEKIIDVDFIIFVCSTHGDGTEPFNMTKFWSFLSNDSLPSTILSHLSFAVFGLGDSSYEKFNYCSKRLFNRLKMLGGRPVIRRGCGDSQDKEGYLSDLRPWLLELVTYMKSYRTKDIILIPPKPEEYISRLVGKRQLTPEDHFQKIVEFVFDIPGYKDFSPGDCLSFLPENYNYKEFMSYNGIEEEEMDGVLSTWLVRQVIDFNSQPHQPFFFALGHFLRQKIDVEEEILLKVEEIAEDYDLYYDYIIKPKRTVFEVLKDLKVKVDIAFLRRFGPTLYPRFFSVTKKEGLYHITASIVRYKTLLAQPRKGVCSEYLMSMSLNNTVRVGVGRSNLYFGSDKLLFICTGTGIALPRACVHEFKDKEMVIFYGFRHRHKDFLYSDEWNNKNVRIITAASRDDKVYVQDIFRKNPVEGIDQYLIFVSGSSRLNKVVRGVLQDVYGKVVVFQSETW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIF5</accession>
    <name>I6ZIF5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_050710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264500.1">
        <property type="nucleotide sequence ID" value="XM_009266225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIF5"/>
    <dbReference type="GeneID" id="20521303"/>
    <dbReference type="KEGG" id="ero:EROM_050710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050710"/>
    <dbReference type="HOGENOM" id="CLU_506247_0_0_1"/>
    <dbReference type="OrthoDB" id="2192946at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF13">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2 4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="5">
        <location>
            <begin position="21"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="540" mass="63760" checksum="D47C573E2C3247D3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIG8</accession>
    <name>I6ZIG8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050860</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264515.1">
        <property type="nucleotide sequence ID" value="XM_009266240.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIG8"/>
    <dbReference type="GeneID" id="20521318"/>
    <dbReference type="KEGG" id="ero:EROM_050860"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050860"/>
    <dbReference type="HOGENOM" id="CLU_976946_0_0_1"/>
    <dbReference type="OrthoDB" id="2191625at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="298" mass="34937" checksum="C9CAFCA854EE4723" modified="2012-10-03" version="1">MEEKIKSLEKKLLAAEQLHRKCEGFKLMNSRIKEYKSRLEALDSRIRSIDTQIAGYDLVDLLGDKSADINSMDLELVVSVMKDMLAANLRFKEDGSTEYLEKCDILWKKIRKVGFLRLNEIIYKSTESLIMNPNFTEFIKLLDESLVYRIQVKILQSRKVECLRKSVHIKRNREFLFKSMIQQELYIFLSLFPWEAKRLDKRLRGFKEERPLAESGLFECFSFSVLKEFFESCTMEDLESLKNRLETDLESSVTNLSNEGEVNEHGDFYANVLMFISVRHYLSSRQDYGGIQGEIVEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZIX6</accession>
    <name>I6ZIX6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_060490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264640.1">
        <property type="nucleotide sequence ID" value="XM_009266365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZIX6"/>
    <dbReference type="GeneID" id="20521446"/>
    <dbReference type="KEGG" id="ero:EROM_060490"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060490"/>
    <dbReference type="HOGENOM" id="CLU_142965_0_0_1"/>
    <dbReference type="OrthoDB" id="2195007at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="155" mass="18550" checksum="E694BBE049A524A8" modified="2012-10-03" version="1">MCNEPEDTHTVGMKRFSQNQTSTLPSGCREEYKKIVYVSHPTIPDKKIKYRLCYTKSSGYESESNEVLRIEISHDLENLAHYIRKILDYHRFKRLKIYVKYSEFLKLHKEIEKVNNDLFTCNYTFKFSFIPSSYKEEEYVLLYEIEGIKRGEHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ59</accession>
    <name>I6ZJ59_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">GCN1-like translational activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070070</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264755.1">
        <property type="nucleotide sequence ID" value="XM_009266480.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521565"/>
    <dbReference type="KEGG" id="ero:EROM_070070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070070"/>
    <dbReference type="HOGENOM" id="CLU_260015_0_0_1"/>
    <dbReference type="OrthoDB" id="5148094at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1319" mass="152195" checksum="42E231BD1577551C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ62</accession>
    <name>I6ZJ62_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">26S proteasome regulatory subunit 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_070120</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264760.1">
        <property type="nucleotide sequence ID" value="XM_009266485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJ62"/>
    <dbReference type="GeneID" id="20521570"/>
    <dbReference type="KEGG" id="ero:EROM_070120"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070120"/>
    <dbReference type="HOGENOM" id="CLU_000688_2_2_1"/>
    <dbReference type="OrthoDB" id="1664597at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000034">
        <property type="entry name" value="26S protease regulatory subunit 10B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0647">Proteasome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="168"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="391" mass="44326" checksum="DFA9AAF2741B3AD6" modified="2012-10-03" version="1">MFQEEKNKLYEEYAQALLSNEKAYQALKMEQEVYRQTHKHYLKSENDVKALQTVGHIIGEVLEKLSDEKFIVKSSQGPRYVVGCMKAIDRKNITTGTRVALDITTLTIMVVLPREVDPMIHSMGEESPGDDISFESVGGLKDQIRELREVIELPLKNPDIFKRIGVRAPKGVLLYGPPGTGKTLLARIVAATMDVNFLKVVSSALIEKYIGESSRMIREMFAYARRKAPCIIFMDEIDAIGGKRSRESSSSDREVQRTLMELLNQLDGFNELDNVKVIMATNRPDILDPALLRPGRLDRKIEIPLPNEQGRKEVLKIHSRPMNAIEEIDYDSLVKLTAGFNGADLRNVCTEAGMIALKDERDYVKHEDFVKSARKIADAKKLETRLDYKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJH4</accession>
    <name>I6ZJH4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264890.1">
        <property type="nucleotide sequence ID" value="XM_009266615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJH4"/>
    <dbReference type="GeneID" id="20521704"/>
    <dbReference type="KEGG" id="ero:EROM_071420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071420"/>
    <dbReference type="HOGENOM" id="CLU_1261501_0_0_1"/>
    <dbReference type="OrthoDB" id="2192467at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="221" mass="25614" checksum="E10CF2741535B34D" modified="2012-10-03" version="1">MGSKRFVRYLSDSIASLGSMVMSMSPNSRAPVEESSVPLSSINMSEKTQDCESIANHSDEETVIVKKSDHEKSLFELTSSRVVIEDLKRREEEYSRMLKNYDATLTYVLERNEKEKALLEGVVMNEEIERLKSNEYRLKSYIQALKRDLFASEDKIESLQRANNERMEFLAKQVEEIRGMYEEEKGKVSCLSKKLSELENAFGARCNEMVELVEYCKYLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJX9</accession>
    <name>I6ZJX9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_081520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265065.1">
        <property type="nucleotide sequence ID" value="XM_009266790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZJX9"/>
    <dbReference type="GeneID" id="20521887"/>
    <dbReference type="KEGG" id="ero:EROM_081520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081520"/>
    <dbReference type="HOGENOM" id="CLU_1320881_0_0_1"/>
    <dbReference type="OrthoDB" id="271937at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pus10-like C-terminal" evidence="4">
        <location>
            <begin position="76"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF21238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="208" mass="23962" checksum="79E3759E2361C907" modified="2012-10-03" version="1">MNEEQILYVVESIIKSQPDEMYENKIYSVNDGSKGRIDKAILKSKLPQGDWNTPHTEIMVKVDDDVKIVVQNSPMYIYEEYIKMDRNMTQSPLIVDGHLKCSRSVSDFGVQVKEFFLSDDVVFTPVGREDFDVRMVEGRPLLLIVKNPRRNLSFEALKLALYDGLEIRDLCVVKKECKGTIFQGESLSNKTYSAFLCSERSLTYRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK24</accession>
    <name>I6ZK24_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265110.1">
        <property type="nucleotide sequence ID" value="XM_009266835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK24"/>
    <dbReference type="GeneID" id="20521934"/>
    <dbReference type="KEGG" id="ero:EROM_081990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081990"/>
    <dbReference type="HOGENOM" id="CLU_1030949_0_0_1"/>
    <dbReference type="OrthoDB" id="2191678at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="260" mass="28800" checksum="98722DCABC484506" modified="2012-10-03" version="1">MRLLILYMTSLVMGSRMVRITSRNHPNLGIVIVPSKANGKGSVRMLSTKENRKMEQQGTFKINKGMTEVSQGESKLCKNEKNDDIDVCTGSDELYSKWRLVSEDGGVRFKTENDICLETVEDQSVSGGVRLAGRECKLNTKSQLFDLKFAEEDSQDSDEAEFSKSSPKDLPLEELLPFLLYGKEPVSNQRIILYDSQGKKKASFKGPVSPLMVASLSSGILPHSNKVSAPYSGSRPLSNPNAPENEVAITPNYADYGKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK64</accession>
    <name>I6ZK64_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cdc6/ORC1-like ATPase lid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265135.1">
        <property type="nucleotide sequence ID" value="XM_009266860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK64"/>
    <dbReference type="GeneID" id="20521959"/>
    <dbReference type="KEGG" id="ero:EROM_090200"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090200"/>
    <dbReference type="HOGENOM" id="CLU_1652136_0_0_1"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054425">
        <property type="entry name" value="Cdc6_ORC1-like_ATPase_lid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22606">
        <property type="entry name" value="Cdc6-ORC-like_ATPase_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cdc6/ORC1-like ATPase lid" evidence="2">
        <location>
            <begin position="29"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF22606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="160" mass="18608" checksum="4C687F392271BFFC" modified="2012-10-03" version="1">MYHLKDRQKLITISNDLRMSGDVLFFKPYTSSDMEKILTYKISKETTSRVLSPVAIKIISKRIGPSGDLRQLFRYVQEIVGRKIIEGGSAEIGPKDVSPEKENREEGPNNIHHSIISSIIVKNKRASRMEVYSKYLRECQEMRIPFYDRTDFNIIYDIYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK70</accession>
    <name>I6ZK70_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265145.1">
        <property type="nucleotide sequence ID" value="XM_009266870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK70"/>
    <dbReference type="GeneID" id="20521969"/>
    <dbReference type="KEGG" id="ero:EROM_090310"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090310"/>
    <dbReference type="HOGENOM" id="CLU_683397_0_0_1"/>
    <dbReference type="OrthoDB" id="2191910at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="414" mass="48100" checksum="9FCF15B1C4B1FF74" modified="2012-10-03" version="1">MSVNYIYTNAWKCVQRCINDHEDYRMKEEDSFEYSTENDYKRGSANGAAARISVENIILVKTDELERKIRINYNVFKNSLHFFLKFRDFLKELAALSEKLVVQIDDVDKTLFLNFLLDSFNLFGEVEGLIDVFRPTRLTPVQRSGLTSEEMLRTLFLLQNIDDGMRVFLSFNVFAISIYHGDRYVGSLLDNIYKKIIVKLLFKKRSSYEDPSPEAHGKHGVERLEFAMKRCMMVKPLLNGLYLLFDIFNVDDVSLRDIFRILLTLVTKERGMKLVNFLGLNDLFICAVKSICEDLVPSHLLMIACRDKLNMKYKAASMGLSIDYVYNWIYKPHLTIMMPQWFSRSRSEGRNLLVMGRPIEAISDKVTVEFTKEDKESSSARGPAYSPLSRRLSTGLRDRSPNRFRSKQKLDFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZK82</accession>
    <name>I6ZK82_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090510</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265165.1">
        <property type="nucleotide sequence ID" value="XM_009266890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZK82"/>
    <dbReference type="GeneID" id="20521990"/>
    <dbReference type="KEGG" id="ero:EROM_090510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090510"/>
    <dbReference type="HOGENOM" id="CLU_842060_0_0_1"/>
    <dbReference type="OrthoDB" id="2190441at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="330" mass="38907" checksum="618CD909C088C3DD" modified="2012-10-03" version="1">MKFNSADFPIMNRMIVAREDGIHYEMEKNSLADTWDRLLEYYSEKILEEHREEVEEEELYRMLSKARIEVETGGRVYELMEKGVLDTYKVRPRHVDRCEFKGYILDTCIRLLKKGYSELENAIKKEARVWRSVEGIGMPTKLVNGEVRVYVTSTQYLTVTEEGDYICPTDWKPRGLTCSIRYMDVRLEYSALPKDDMNRIEMAKEYFFEKSIVQEFSRNDVEIKISEMEKATEKNDMICMYVIKELKGGKNIREIFCMVSLYLSRKLVDIIISGHKECKVLKYNGCILKDGLPEVFFVLAGNDVFKIIRRIPDRFEIFVNEIPIKSFRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKD8</accession>
    <name>I6ZKD8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative mitochondrial translocase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_091270</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the Tom70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265240.1">
        <property type="nucleotide sequence ID" value="XM_009266965.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKD8"/>
    <dbReference type="GeneID" id="20564351"/>
    <dbReference type="KEGG" id="ero:EROM_091270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091270"/>
    <dbReference type="HOGENOM" id="CLU_573664_0_0_1"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46208:SF1">
        <property type="entry name" value="MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN83743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="477" mass="55197" checksum="99AED4D1F52130DC" modified="2012-10-03" version="1">MVGRKTSAFLAIAIVSGAVLVCQGVRTWRRNRRERNIREYMERGLLLLKEKVYGEAALSYLKCLSLMEEEDGRLVSVYNNLAICLAKVRAYKEAALYAERSLKINILDNEKALRLRYECHKALDMRRETLCDAFLCGLVTKDEKYRKLAQEILKLEAEGEAKKMAGVKEASPSSISYENFFGTFPDLFGNESFVNDELVRLVRSGKYDEAFEKAEKRDDSISKFVAAGVIHVRGKDLSSISLLEHEKMIFSICLREYLKSLHGHTPMDIEDFVLKNSKSVSVLFYASKIHLNLKNHELYEKFIDMAMECEEHDFLYVDRIIYEVGHGRNERAQEFLNKALESHPLSIHVLSIACEYYLRCKDMTRLETTMSNFEKYYHEDPRFLLFKGIVAQARNEVSDAERYFRLAIEADSKFFKPYVYLGGILLGRNDKSCREVYEEALKCAASYDELFLVEQALILIDVQEKIIRIYPDVLKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKE9</accession>
    <name>I6ZKE9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_091420</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265255.1">
        <property type="nucleotide sequence ID" value="XM_009266980.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKE9"/>
    <dbReference type="GeneID" id="20564366"/>
    <dbReference type="KEGG" id="ero:EROM_091420"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091420"/>
    <dbReference type="HOGENOM" id="CLU_155991_2_0_1"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="8">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="93" mass="10897" checksum="D1700C2BB36FDDA2" modified="2012-10-03" version="1">MNFRPEYKEYFKQGSTAKIYNGAFEDMGFYDEVDIKEFKYSKEDRTFYYPCPCGDSFEISLEDLKNGEVVARCPSCSLIICTVYEVEDLEAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL94</accession>
    <name>I6ZL94_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">V-type proton ATPase catalytic subunit A</fullName>
            <ecNumber evidence="2">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_111170</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (components A to H) attached to an integral membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and VOA1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Vacuole membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265595.1">
        <property type="nucleotide sequence ID" value="XM_009267320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL94"/>
    <dbReference type="GeneID" id="20564716"/>
    <dbReference type="KEGG" id="ero:EROM_111170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111170"/>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_1"/>
    <dbReference type="OrthoDB" id="1676488at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033180">
        <property type="term" value="C:proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055082">
        <property type="term" value="P:intracellular chemical homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000052">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005725">
        <property type="entry name" value="ATPase_V1-cplx_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01042">
        <property type="entry name" value="V-ATPase_V1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="15">
        <location>
            <begin position="28"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="16">
        <location>
            <begin position="131"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="14">
        <location>
            <begin position="235"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="17">
        <location>
            <begin position="465"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN84098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="611" mass="68014" checksum="1E7FE46D78A93BB1" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZLH3</accession>
    <name>I6ZLH3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Chromatin elongation factor SPT5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Chromatin elongation factor spt5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">EROM_111820</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">The SPT4-SPT5 complex mediates both activation and inhibition of transcription elongation, and plays a role in pre-mRNA processing. This complex seems to be important for the stability of the RNA polymerase II elongation machinery on the chromatin template but not for the inherent ability of this machinery to translocate down the gene.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265660.1">
        <property type="nucleotide sequence ID" value="XM_009267385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZLH3"/>
    <dbReference type="GeneID" id="20564781"/>
    <dbReference type="KEGG" id="ero:EROM_111820"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111820"/>
    <dbReference type="HOGENOM" id="CLU_034563_0_0_1"/>
    <dbReference type="OrthoDB" id="28901at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032044">
        <property type="term" value="C:DSIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06081">
        <property type="entry name" value="KOW_Spt5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06085">
        <property type="entry name" value="KOW_Spt5_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09888">
        <property type="entry name" value="NGN_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041973">
        <property type="entry name" value="KOW_Spt5_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041975">
        <property type="entry name" value="KOW_Spt5_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041978">
        <property type="entry name" value="KOW_Spt5_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057934">
        <property type="entry name" value="KOW_Spt5_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039385">
        <property type="entry name" value="NGN_Euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23284">
        <property type="entry name" value="KOW2_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23290">
        <property type="entry name" value="KOW5_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23287">
        <property type="entry name" value="KOW7_SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="14" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="KOW" evidence="13">
        <location>
            <begin position="164"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="13">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="13">
        <location>
            <begin position="313"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="13">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="13">
        <location>
            <begin position="522"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="AFN84163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="573" mass="65034" checksum="FB8B74E2A605727F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZRZ5</accession>
    <name>I6ZRZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rcd1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010160</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009265583.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZRZ5"/>
    <dbReference type="GeneID" id="20520642"/>
    <dbReference type="KEGG" id="ero:EROM_010160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010160"/>
    <dbReference type="HOGENOM" id="CLU_039962_2_2_1"/>
    <dbReference type="OrthoDB" id="1183224at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030014">
        <property type="term" value="C:CCR4-NOT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM-like"/>
    </dbReference>
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        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007216">
        <property type="entry name" value="CNOT9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12262">
        <property type="entry name" value="CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04078">
        <property type="entry name" value="Rcd1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="280" mass="31550" checksum="529C563BBC445859" modified="2012-10-03" version="1">MEDGVKDNRHEATQKQRLSDLCKLVISNSKDINKLDELFEELKKSPGLSLDIWTHDSMPIFLLQEVITPYMILSTSKFDEVQYHRLYTVLNILQILVSDSRIKRVFVDARFPYYIYRYLVISDSGSKYETLRISALGVVASLLQNGDQYVHKQLKTTEVVPLLLKIVDLGSEASQLLSANVFGLIIGSDDGLNYACQTFDRFSAINLMFNSLASQGVSIGSVKLVKAALRIYIRLCAKPHIRALLSSKKPDGLFTDEAGTLISSDAECRMLFKSFMDMIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZS68</accession>
    <name>I6ZS68_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Spindle assembly abnormal protein 6 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_011020</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263943.1">
        <property type="nucleotide sequence ID" value="XM_009265668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZS68"/>
    <dbReference type="GeneID" id="20520729"/>
    <dbReference type="KEGG" id="ero:EROM_011020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_011020"/>
    <dbReference type="HOGENOM" id="CLU_738101_0_0_1"/>
    <dbReference type="OrthoDB" id="49058at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.210.20">
        <property type="entry name" value="Spindle assembly abnormal protein 6, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032396">
        <property type="entry name" value="SAS-6_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038558">
        <property type="entry name" value="SAS-6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44281:SF2">
        <property type="entry name" value="SPINDLE ASSEMBLY ABNORMAL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16531">
        <property type="entry name" value="SAS-6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spindle assembly abnormal protein 6 N-terminal" evidence="7">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="187"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="376" mass="44524" checksum="18ECF3770199B977" modified="2012-10-03" version="1">MRESIFTGKIPISCQNEEIPWQMKCDIVRSDDEVEIRLIDTRDIFTFYFSTISPGDFYVMKREQDIIVDYEKFVSILVKMFHSLHSGKLRGLFSKETSKFLFVEKDEFRNIVRLELKFSKPDETHYKMYLGDMIGRMEADNVRLIKENGMLRDKCKNGEKELRDRIRYLEEETMASQKKMNKLYRDNEVLSEKGNSQREEIERLNSKIYDLEKENGRLQYELDKLKLQDVKNSGLVERAQKIEDENKMLKEELSTANEIIRKYNDEITQMKNAINGNEDATKDLRLNNKKYKSEIEDLKKRVKGMEEKMKKIKEELKAKETRLKDLETENMGLVKNLENAQNVYSHFYSKNIENPTSINYSDNESIFSIAPESSPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSA6</accession>
    <name>I6ZSA6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polyadenylate-binding-like RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_020160</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263988.1">
        <property type="nucleotide sequence ID" value="XM_009265713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSA6"/>
    <dbReference type="GeneID" id="20520774"/>
    <dbReference type="KEGG" id="ero:EROM_020160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020160"/>
    <dbReference type="HOGENOM" id="CLU_1215098_0_0_1"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="81"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="167"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="247" mass="28360" checksum="C115FFD97518D262" modified="2012-10-03" version="1">MASKLFVSNFPESYSEENINLIFSPFGEIRSIKILREPRMFSIVEFRDPLDAKAAIDSLNGRRLHGEGLSLHIEMAFDRKRRVIVSGIPSDSSSEELMRFFQYYGPIENITSSDHPQTIFIDFLSHKDAEMFLELNQKISFGKEKDRLSIKPFTKRERRDHISSPERSIFIYNLPSKTTKTDLNDLFTKFGEIQSLGILSGGKAFVNYERELSALKAIRHMDGKIVGGNKVRIVLKSMKKGMFKRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZSX3</accession>
    <name>I6ZSX3_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_030650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264183.1">
        <property type="nucleotide sequence ID" value="XM_009265908.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZSX3"/>
    <dbReference type="GeneID" id="20520976"/>
    <dbReference type="KEGG" id="ero:EROM_030650"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030650"/>
    <dbReference type="HOGENOM" id="CLU_106358_0_0_1"/>
    <dbReference type="OrthoDB" id="2189627at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="232" mass="26566" checksum="DED2AADFA5578F6B" modified="2012-10-03" version="1">MGRERMTIVTLRSRNGEYTYTKEFESDHTILHVKEYLKSQIEEYSIESTEKGIKIILNGKIVSDAVQLHTLGKGSLQLVYDVPPQKVGERYKGGGDVLCEEIETNDISIKIAVSVKETGAKIMVDPEDLVMFNGEVYLVKERKRILTLKSIMDLLGSIRISREDLAKFLIFFLLLYTGNGGIVLVLGCVFVLELLSRRLSLTHQNHFKSMDHFCRSFYMFFVSLFLIDHGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTC9</accession>
    <name>I6ZTC9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">40S ribosomal protein S10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_041350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264398.1">
        <property type="nucleotide sequence ID" value="XM_009266123.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTC9"/>
    <dbReference type="GeneID" id="20521198"/>
    <dbReference type="KEGG" id="ero:EROM_041350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041350"/>
    <dbReference type="HOGENOM" id="CLU_2359720_0_0_1"/>
    <dbReference type="OrthoDB" id="5211809at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005326">
        <property type="entry name" value="Plectin_eS10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037447">
        <property type="entry name" value="Ribosomal_eS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12146:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03501">
        <property type="entry name" value="S10_plectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Plectin/eS10 N-terminal" evidence="6">
        <location>
            <begin position="3"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="96" mass="11135" checksum="E3A8C33472157513" modified="2012-10-03" version="1">MLIPTPNKYKIKRRLLEKKALLLEDTVLGNHRDLEIPNLQLKVFMKTMISHGYVQKVHIWRHSYFLLTSLGESRLREELVFTDEGMANQEASAAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTU1</accession>
    <name>I6ZTU1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcription-associated recombination protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_051610</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264588.1">
        <property type="nucleotide sequence ID" value="XM_009266313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTU1"/>
    <dbReference type="GeneID" id="20521393"/>
    <dbReference type="KEGG" id="ero:EROM_051610"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051610"/>
    <dbReference type="HOGENOM" id="CLU_062465_0_0_1"/>
    <dbReference type="OrthoDB" id="1713558at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008180">
        <property type="term" value="C:COP9 signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050756">
        <property type="entry name" value="CSN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 3/COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10758:SF1">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00753">
        <property type="entry name" value="PAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="5">
        <location>
            <begin position="159"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="376" mass="43762" checksum="E35048E222338FE8" modified="2012-10-03" version="1">MSEREYVEELVEVLGQLSTNREEAMEQYERRALTFMKNISPETLDSLSFDNEFERIATYPIILGGLFMKKEFKRIDKVVNEGLLPYLIGKKRTYDYFIGLIVKFFYLARKNECQDNSTLFSLLVTNKELGNEYTISVITNCLLDMLISNKIFQRIENTIATTSEQARYNYYNGIISMVEGDYESALKNFHTSIILSSNRDLVLGAEKRVILCMLLSSDYNIPYPYKPSLRTYFELASVVKKAEIKKFEETLENSKDELMAQGLYFVARRLSQNVIQEGIRRISIVYSRISFVDIAYLLDINPEEAEYLVKRTIRKGLIKGKAIDGIFHSLKEDKPVADIGMSIRDCIQLTKYIQEHMRYPAIEPLCYEKVRKVYDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU72</accession>
    <name>I6ZU72_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">U6-snRNA associated small nuclear ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_061090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264698.1">
        <property type="nucleotide sequence ID" value="XM_009266423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU72"/>
    <dbReference type="GeneID" id="20521505"/>
    <dbReference type="KEGG" id="ero:EROM_061090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061090"/>
    <dbReference type="HOGENOM" id="CLU_187872_0_0_1"/>
    <dbReference type="OrthoDB" id="2020720at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00600">
        <property type="entry name" value="Sm_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001163">
        <property type="entry name" value="Sm_dom_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01423">
        <property type="entry name" value="LSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="71" mass="7892" checksum="204890047C075C8C" modified="2012-10-03" version="1">MDNTLYTAFIGRVVKVKICDSSEFRGTSVAVDAYLNISLESVSYIQSGQEPRHYSSLFIKGSYIDHIALDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUE1</accession>
    <name>I6ZUE1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264788.1">
        <property type="nucleotide sequence ID" value="XM_009266513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUE1"/>
    <dbReference type="GeneID" id="20521599"/>
    <dbReference type="KEGG" id="ero:EROM_070400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070400"/>
    <dbReference type="HOGENOM" id="CLU_1602704_0_0_1"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="2">
        <location>
            <begin position="59"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="166" mass="18946" checksum="0EEE9ABDBD6D8A17" modified="2012-10-03" version="1">MVDFFLLNERAFTGNCMHVVLIGGYKDKIFSPQPGMADVDRDNIAKGVEKPPLRGADNAVLTNIVSQGLITFCILIVYIIFRKRAKWLYSPNVKDRPSHPCYNYAGFLSWIIPVVTVSDTVLLSIIGLDAFMMLQTLKMLYRILFILSFFVIPSLGYLFWTNQREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUK9</accession>
    <name>I6ZUK9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">20S proteasome subunit beta type-1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_071350</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264883.1">
        <property type="nucleotide sequence ID" value="XM_009266608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUK9"/>
    <dbReference type="GeneID" id="20521695"/>
    <dbReference type="KEGG" id="ero:EROM_071350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071350"/>
    <dbReference type="HOGENOM" id="CLU_035750_1_1_1"/>
    <dbReference type="OrthoDB" id="268479at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF2">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="289" mass="32690" checksum="110EA3DA1378D763" modified="2012-10-03" version="1">MLNNKGRAYSTSGGSILNTVREYGLFEDLDIHTPLNSAVMLDDGRDEDSVERMGKGHLGESMESLFISGRSRYTKAEDGEKFNPYEDNSGTVVCLRQDDFIVIGGDTRHSSDMIINSREMTKIFQIGDLFLTGTGFYADSHEVYVKMAYEIRQYEVEGSINVHSAANLLSKILYSKRFFPYYSYCVLSGFENGRPYVYSYDPVGSFESVTCICNGSGRSMIQPLLDSFVSKKNWNNAGQAQLSQEDCVRLITKAFNAAAERDVKTKDNLEVYVVRENEVERKVFPLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUW3</accession>
    <name>I6ZUW3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_081150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265028.1">
        <property type="nucleotide sequence ID" value="XM_009266753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUW3"/>
    <dbReference type="GeneID" id="20521850"/>
    <dbReference type="KEGG" id="ero:EROM_081150"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081150"/>
    <dbReference type="HOGENOM" id="CLU_885744_0_0_1"/>
    <dbReference type="OrthoDB" id="2191885at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="314" mass="35367" checksum="A083FDCE672A7C1B" modified="2012-10-03" version="1">MNTSISPFVLLAIMLILGATLVNLIALRANNKAEPAGTRYIEKIKSTRKKGMIFFLLALIFTTVYYFFYTQNHLEGRTPPLLKRVSVNSHSLKMLELSARSFQKSFMFYATSLIIPLVSSRRFRVLGIDEKTSIAFVKYYSLLGSFLYPLVFHVCSGHDLGSYLVEVLNIAETSLLLSLYSLLLLKMCKVHDVLSYTQVITKFEIEEVLDRVKPKIIGIIGYLCLDGASGVARMTAMFFAGDYSRGLPVNSLVETAFFIALYYYSIKFLVTPGNGICESIQKMENIKKHVAAGFLNLEHRDEDSMIDVSKIPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUX4</accession>
    <name>I6ZUX4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265043.1">
        <property type="nucleotide sequence ID" value="XM_009266768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUX4"/>
    <dbReference type="GeneID" id="20521865"/>
    <dbReference type="KEGG" id="ero:EROM_081300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081300"/>
    <dbReference type="HOGENOM" id="CLU_607057_0_0_1"/>
    <dbReference type="OrthoDB" id="2191648at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="455" mass="53662" checksum="1896238F82DCE258" modified="2012-10-03" version="1">MPTLFSEYLSCGRSLMVPVKARIFRSVNDEWEEICMGMIFRIEKDCIFIEDDGTGKCFEMSLGKKQCERSEKNIVIVYNDLEEHAVCFETEDIRDDFVEFMEKDVLSKDGMSTEGRMTFEKESNLFSNLLRISRYMDGSVFGRMLESRDGVLNLLRMEDFHLFRMLLENKEKVFELFGVVLNSKINKVTPHGFYREIIAKNLDESTARTYENFLIMINKQELTRNESKVTEMSDEEIRSFLKRCGSNVCRVGNIDVYLERIYRDNNYFCETFYYLCFIFRERMSEVVDLGHILCRTRNLIGEKTFDDGLLYVLEGLYILLDECKPEKLDMFYMEVSGLFDNLECHPDLQNFLIYLLINHGFRARELLVNTGLMERIFISGCESNMSSVFISKVLLQIVSCSSRFIHRYFIKNDLFRGVVRMYNQRKEDAAYSIFLQAFTQADGDMKIYLDRYIQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZV47</accession>
    <name>I6ZV47_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265118.1">
        <property type="nucleotide sequence ID" value="XM_009266843.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZV47"/>
    <dbReference type="GeneID" id="20521942"/>
    <dbReference type="KEGG" id="ero:EROM_090030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090030"/>
    <dbReference type="HOGENOM" id="CLU_1081942_0_0_1"/>
    <dbReference type="OrthoDB" id="2195534at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="251" mass="27360" checksum="0BD485EB68F1B65E" modified="2012-10-03" version="1">MPSLMLGLFKRNETLESLILIGVVLAEMRLNRIESSGVGFQIFSLGAVAFFVVRVLVTISNVELGDVNTLGSIAVASSVFLSVIGLLQAICSVVNMKSDRGKLFYSKEGKRLIAGGYATIVAAIFLLVQTSKIIKTKNTIERRDFMNGLLMMVAATVYFFLADDIDQEQLSSIIICLALVVALLLLVSSNNKEENKVPNKGILSYLMASYIGWFIASALGIQVMGDKVKMVEKLVAIFTMFDKAISILLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVF4</accession>
    <name>I6ZVF4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_090950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265208.1">
        <property type="nucleotide sequence ID" value="XM_009266933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVF4"/>
    <dbReference type="GeneID" id="20564319"/>
    <dbReference type="KEGG" id="ero:EROM_090950"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090950"/>
    <dbReference type="HOGENOM" id="CLU_1065699_0_0_1"/>
    <dbReference type="OrthoDB" id="2190726at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="261" mass="29040" checksum="30311FECF29EC683" modified="2012-10-03" version="1">MIAMLFLGYAAATEGVPPDIWDSMGYYGDFLSERREIAIALFVSGLLVNFLGICAKRCSLSVIAFPVLRLGIGSIMEVVDKIIADKYGTAENAPKAIGHVVLFLKKVDANRFFMAFLSVALGIVMFFFVKTIIYILMGYLAFHLWGILESSTGETMEGSSYYIYQTIMLCIGILVLVLVKPITFLIYILVFSVFGSLFIIVGANFGFGLNLEFDQYIATLRRGNFYQGLVENKISVGYISLITMGVVSQILLRKKGERKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVF8</accession>
    <name>I6ZVF8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_091000</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265213.1">
        <property type="nucleotide sequence ID" value="XM_009266938.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVF8"/>
    <dbReference type="GeneID" id="20564324"/>
    <dbReference type="KEGG" id="ero:EROM_091000"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091000"/>
    <dbReference type="HOGENOM" id="CLU_842250_0_0_1"/>
    <dbReference type="OrthoDB" id="2190998at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="331" mass="38667" checksum="73A079139BFD06A9" modified="2012-10-03" version="1">MDFEIYSSDVYTGGVFKSCIKWKRCGYTSQKPGLLAARITGIVRIDGKEVKFLESTPVEIQMGEGIETIYRCTIPRNALPSMRLKSLEVVYMIIVEGYYGTKKDIRSEIFDVYPIGLEPPRKMESMIVHGDMIRIGDGDSTAFGEIVNSLRNKSEWSSGTIAEMVEQRMRILEEFPRFMNKVVERYREEIKGDIYFYVSMEDPKIEEDRHRVTAWNGDEEIAKIEYRKYLIENDTVEIWYKRNIKSTRITLRITEYIDGEISNDEEKVMKEFNSNMCLYKMVPLIIKHDWFTFDCTLFTIKFSYRIEFDGVGFVLPICKVSPKARKSIGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVJ7</accession>
    <name>I6ZVJ7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">G2/mitotic specific cyclin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_091450</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the cyclin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265258.1">
        <property type="nucleotide sequence ID" value="XM_009266983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVJ7"/>
    <dbReference type="GeneID" id="20564369"/>
    <dbReference type="KEGG" id="ero:EROM_091450"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091450"/>
    <dbReference type="HOGENOM" id="CLU_1277599_0_0_1"/>
    <dbReference type="OrthoDB" id="5590282at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000010">
        <property type="entry name" value="G1/S-specific cyclin Cln1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0132">Cell division</keyword>
    <keyword evidence="3 5" id="KW-0195">Cyclin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="6">
        <location>
            <begin position="32"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN83761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="208" mass="24552" checksum="84204D142E4F8924" modified="2012-10-03" version="1">MMKRVFQDITNVSRKNIKLTIHKSSHRRKLVKWLYEVMDDFGYSQVTFSTAVFILDKYTETRGLDLGKYQLVGISALFLSAKIEEKRCKGIEDYVHVTDNTYSKEEILDKEVEMLEIFGFNLMFRLPHSFLKERYLEKASGKYTVKQSQEIFFCAFSYVLEKNICGSSMYWIYLEGVKEAEKILSGNEIDAGLRFYLENNRRIKNIFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACS1</accession>
    <name>I7ACS1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACS1"/>
    <dbReference type="GeneID" id="20520662"/>
    <dbReference type="KEGG" id="ero:EROM_010350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010350"/>
    <dbReference type="HOGENOM" id="CLU_463818_0_0_1"/>
    <dbReference type="OrthoDB" id="2190892at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="19"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="589" mass="70276" checksum="B66259467E62FE26" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ACU8</accession>
    <name>I7ACU8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Trehalose-6-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_010660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263907.1">
        <property type="nucleotide sequence ID" value="XM_009265632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ACU8"/>
    <dbReference type="GeneID" id="20520693"/>
    <dbReference type="KEGG" id="ero:EROM_010660"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010660"/>
    <dbReference type="HOGENOM" id="CLU_002351_3_3_1"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF106">
        <property type="entry name" value="BCDNA.GH08860"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="444"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="717" mass="82539" checksum="41BAF0F54A4364A0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD31</accession>
    <name>I7AD31_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_020350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264007.1">
        <property type="nucleotide sequence ID" value="XM_009265732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD31"/>
    <dbReference type="GeneID" id="20520793"/>
    <dbReference type="KEGG" id="ero:EROM_020350"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020350"/>
    <dbReference type="HOGENOM" id="CLU_029507_0_1_1"/>
    <dbReference type="OrthoDB" id="446890at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF78">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 5 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000303-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN82510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="173" mass="19990" checksum="A5FD30C7908EA4F1" modified="2012-10-03" version="1">MEKDMESEAFYSLSARKWDGSKVDFETFKGCVLIIVNVASSCKLAESNYKSFVNLLSKFYKKGLRILLFPCNQYLKQESKPIEEIHEGIVKKYSDKFEIFDKIDVFGEEIHPVFKHLINTKNGKGILGKFIKWNFTKFLIDRQGHVVKRVGPIGIVKEDDKCLLNSIEDDGRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AD46</accession>
    <name>I7AD46_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_020500</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264022.1">
        <property type="nucleotide sequence ID" value="XM_009265747.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AD46"/>
    <dbReference type="GeneID" id="20520810"/>
    <dbReference type="KEGG" id="ero:EROM_020500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020500"/>
    <dbReference type="HOGENOM" id="CLU_030571_4_5_1"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="12"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="247" mass="29061" checksum="E053636CE6C038FE" modified="2012-10-03" version="1">MGVKVVSIVVGDNYMHLFYEEEAAFCIDACNPKVLLKALGINFKKKIYSEKEILSMPEDMTKKRRLVYLFTTHKHQDHSCGIPYIMENSPDTQRISGFEGNICKCGDKFHFREVEIECLYTPCHTMDSFCYYVDGRFLATGDTLFFLGCGKFFEGTPEQMIEAIMKIRGRVNNEAVLLYGHDYNEQNIRFTEELYPVPLEIKETKFLRLKDEMEYNPFFNLRRIFTKGSEEEMMGNLRERKNRFNRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADR6</accession>
    <name>I7ADR6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_030990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264217.1">
        <property type="nucleotide sequence ID" value="XM_009265942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADR6"/>
    <dbReference type="GeneID" id="20521011"/>
    <dbReference type="KEGG" id="ero:EROM_030990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030990"/>
    <dbReference type="HOGENOM" id="CLU_087656_0_0_1"/>
    <dbReference type="OrthoDB" id="2195891at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="304" mass="33863" checksum="F7B0A06F1D04555F" modified="2012-10-03" version="1">MTLINIFHMDIFNLGLNDFFVIKNILYMLFRRIQLPNKPLVISSLGDSIYIGDARGYVYEMKPPYITPMVIFQSPGPVSALAIGKQVYYGNWDGDVGIVGGRKINLGNQIVKCMAIHKGRIYVSVGLMIYSLTLGLEIECSYEVKHKVLCMSDFQGSIYCGMGVPFLLRIHNGVEIIGRSLHDTSIFCICGEYTGSADGRVLRQDYSDLDNAEEIYKGDSWIRSMYNQYLFSDGRSVMADLDKLKGNGGGGIKPIYSHEEDVVGVVKVGGKIISIGLDYCYCVFEITPSLSIEEEMEIAELMNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADR9</accession>
    <name>I7ADR9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Calponin H2-like calcium-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264222.1">
        <property type="nucleotide sequence ID" value="XM_009265947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADR9"/>
    <dbReference type="GeneID" id="20521016"/>
    <dbReference type="KEGG" id="ero:EROM_031030"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031030"/>
    <dbReference type="HOGENOM" id="CLU_055232_2_1_1"/>
    <dbReference type="OrthoDB" id="21595at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21210">
        <property type="entry name" value="CH_SCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050606">
        <property type="entry name" value="Calponin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003096">
        <property type="entry name" value="SM22_calponin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385">
        <property type="entry name" value="CALPONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47385:SF14">
        <property type="entry name" value="TRANSGELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00888">
        <property type="entry name" value="SM22CALPONIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
        <property type="entry name" value="Calponin-homology domain, CH-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="1">
        <location>
            <begin position="2"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="172" mass="19860" checksum="C32CF28E5B660462" modified="2012-10-03" version="1">MDKQQKEVTLWIEAVLGEKFGPGTFHDLFKDGVKLCRFLNKVSPLNVKYKESRQIFVQRENICSFINGLKKLNMNEYELFQTNDLFEAKDLKQVVICLYALSRQLQKEKVFSGPFIGPQLSTKSKIEFSKEVLDRSKYAVHLQMGYSDPDLLERTESKAASNTVKDLKTNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADT2</accession>
    <name>I7ADT2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MYG1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_031230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264242.1">
        <property type="nucleotide sequence ID" value="XM_009265967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADT2"/>
    <dbReference type="GeneID" id="20521037"/>
    <dbReference type="KEGG" id="ero:EROM_031230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_031230"/>
    <dbReference type="HOGENOM" id="CLU_051576_0_0_1"/>
    <dbReference type="OrthoDB" id="10265310at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003226">
        <property type="entry name" value="MYG1_exonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="305" mass="35288" checksum="92CD223B8A3D3EE5" modified="2012-10-03" version="1">MKLVTHDERFHYDEVLASCVLLRIYPDAEIMRTRDNAIIEQGDIVYDVGGVFNPATRRFDHHQRTFSETFSSKYNVKLSSSGLIFKYFHKQLLSLYGIEDTCGIYNMVVDKIYSEFFLYADAIDNGQDIYGEIRPRTMADLVGLFNTDTPDEGLENRGFYKVLEIVSTDLDNYMKRIKIWVNNYEHVEKKARETNGPILVLDKHYTTDLILEIESQNGKDFKFMVFPHRNAYRVIAIPKRRGSFETKNPLKKEWRGLVNEELVRASGIEGCIFVHSSGFMGINSTFENALKMCEESLSDGRSSFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADY8</accession>
    <name>I7ADY8_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Probable lysine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Lysyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_040520</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="4"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264317.1">
        <property type="nucleotide sequence ID" value="XM_009266042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADY8"/>
    <dbReference type="GeneID" id="20521116"/>
    <dbReference type="KEGG" id="ero:EROM_040520"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040520"/>
    <dbReference type="HOGENOM" id="CLU_008255_6_0_1"/>
    <dbReference type="OrthoDB" id="21243at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00775">
        <property type="entry name" value="LysRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04322">
        <property type="entry name" value="LysRS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000238">
        <property type="entry name" value="Lysine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00252">
        <property type="entry name" value="Lys_tRNA_synth_class2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002313">
        <property type="entry name" value="Lys-tRNA-ligase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044136">
        <property type="entry name" value="Lys-tRNA-ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018149">
        <property type="entry name" value="Lys-tRNA-synth_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00499">
        <property type="entry name" value="lysS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001756">
        <property type="entry name" value="PRK00484.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918:SF9">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42918">
        <property type="entry name" value="LYSYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00982">
        <property type="entry name" value="TRNASYNTHLYS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="14">
        <location>
            <begin position="110"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN82820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="440" mass="50654" checksum="E9DBC17449E327A1" modified="2012-10-03" version="1">MSIRLHARFCFFMVTSNGENLQLVIDAKEVGKDQIIKFLKRGDIVGFTGKPGRTKTLESSVFVSEVIILSPCLRTVPTEHFGLKDAETIYRRRYMDLLINKESKERFQKRTQIIRYIRRFLDSRGFLEVETPMMNLIPGGAAAKPFVTHHNELKLDLYMRVSPELYLKKLVIGGLDRVYEIGKQFRNEGIDLTHNPEFTSCEFYMAYGDYNDLMVMTEELMSGMVKEMFGTDTIVYSPKKREERSESVELSFKRPFRVISIIEELNSRLGLDLSGETLDSPETLEKLLEACEKEGIHVEKPRTLTRVLDKLIGHVIEPQCVNPTFIKDYPIAMSPLAKNHRSKPGLTERFELFINCKEVCNAYTELNNPFEQRERFIQQAQDMNAGDDEAMMNDEDFCTALEYGLPPTAGWGLGIDRLIMYLTNAANIRDVIFFPTMKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE39</accession>
    <name>I7AE39_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_041240</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264387.1">
        <property type="nucleotide sequence ID" value="XM_009266112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE39"/>
    <dbReference type="GeneID" id="20521187"/>
    <dbReference type="KEGG" id="ero:EROM_041240"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041240"/>
    <dbReference type="HOGENOM" id="CLU_014831_1_0_1"/>
    <dbReference type="OrthoDB" id="10263272at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010997">
        <property type="term" value="F:anaphase-promoting complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990757">
        <property type="term" value="F:ubiquitin ligase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033010">
        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="9">
        <location>
            <begin position="62"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="184"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="225"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="313"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="362" mass="40331" checksum="7386B6F29508FA2F" modified="2012-10-03" version="1">MSINLGDIFSTSRRRQTQKEDRYTKSTMGIHTDVLVSIKLMTSSRSSRIENRHIDTSPHRILDAPGMLDDYYLNLLDWSSTNLVIIGLGESVYGYNVNDKSVLDIHSGENYISSVKSNGDILCIGASDGTMRLIDISVNKEVHTMRNHNARVSSLSWNGNVISSGDKTGKLCNFDIRSGRISMVEGHSQEICGLEWSTDTKYLASGGNDNVIRIWQLGNNNPQTLSGHKSAVKALAWCPWRSGILTSGGGAKDMTIKFWDVAENKLERSIDTQSQVCTLTYLPKYKEIISSHGYIENDIRIWKASTMNLISSFGKHGSRVLHVALSPDGSELASVSADENLKFWKIFNSEKPSTRRDSLSFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AE49</accession>
    <name>I7AE49_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L13A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_041390</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264402.1">
        <property type="nucleotide sequence ID" value="XM_009266127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AE49"/>
    <dbReference type="GeneID" id="20521202"/>
    <dbReference type="KEGG" id="ero:EROM_041390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041390"/>
    <dbReference type="HOGENOM" id="CLU_076922_1_1_1"/>
    <dbReference type="OrthoDB" id="1882297at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="198" mass="22603" checksum="D33238C34D9ADDF0" modified="2012-10-03" version="1">MEKKEHVIIDATGQVAGKLAAKVTKLLLEGVRVSVVCSEEAVFMGSLKRSMDKFKAYLNKRCIVNPRRGPFHFREPRMHLAKIIRRMISYKKARGKIAMSKLATYEGIPRELEGLPRHKVTCALAKYTGNPNNHVTLGKLLSMFGWKHAEAAKSLTEELREREKQAFLKKEEVCKEAEKLKKDKSFEDEVNKKLAMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEB7</accession>
    <name>I7AEB7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RAN specific GTPase-activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_050630</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264492.1">
        <property type="nucleotide sequence ID" value="XM_009266217.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEB7"/>
    <dbReference type="GeneID" id="20521295"/>
    <dbReference type="KEGG" id="ero:EROM_050630"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050630"/>
    <dbReference type="HOGENOM" id="CLU_028747_0_0_1"/>
    <dbReference type="OrthoDB" id="184583at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="355" mass="40477" checksum="738243C847FC363E" modified="2012-10-03" version="1">MIFSIGDQKKKYETREDVSEVVEEIRKSLDELVEVDLSGNHFSEEAMEEICNVLQDAKNLCVINLSSAFLGLDKEKLYSNLVMLSNMLRTHKIQKIDLSDNAISSEFPPEFGEFISSSTDLVHLKMNNCGLGKVGGGRLGNYLLGISDKSKLEVVDIAQNRFFSFPKELSDALLKFENIKELRIQYNTIEEETMLLFLRSFSNHSLEVLDIRDNFLSLEGSRYLGDLYCEWNLKELRVGDCMMGNEGVKEFLRKASKKFIPMSLPGDYNSRRERIILDISYNEFEQDAVELLIDFCRRNTIKELGVFGNYYDDISDVVEIVERQGGIVVTKEQTDLSSDEDLEIDESVIEGIAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I7AED4</accession>
    <name>I7AED4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ham1 nucleotide triphosphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264517.1">
        <property type="nucleotide sequence ID" value="XM_009266242.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AED4"/>
    <dbReference type="GeneID" id="20521320"/>
    <dbReference type="KEGG" id="ero:EROM_050880"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050880"/>
    <dbReference type="HOGENOM" id="CLU_082080_1_0_1"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="224" mass="25185" checksum="0E5287D9936445B2" modified="2012-10-03" version="1">MEDQKTGLKVNMDTIRRSGRIVGREHGLEGGVPKQLRAVFITSSVYKYEAFREFLNAPVELVVLDIKEIQGSKEEIIMDKLQKVSHLVTESTITFIDDTSIHMSGLGGFPGQYAKDFLKIGASRILEIASKVGRECLYSTIIGVMRMEEGKIVTKIFTGEVRGDIIQRSEGEPKTFSDIFVPKEEEYDGIPHGMEGIKIGRYKAVEKVKRYLGEIGVYEQLFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEZ5</accession>
    <name>I7AEZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cdc50-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CDC50/LEM3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264732.1">
        <property type="nucleotide sequence ID" value="XM_009266457.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEZ5"/>
    <dbReference type="GeneID" id="20521541"/>
    <dbReference type="KEGG" id="ero:EROM_061440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061440"/>
    <dbReference type="HOGENOM" id="CLU_090044_0_0_1"/>
    <dbReference type="OrthoDB" id="340608at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005045">
        <property type="entry name" value="CDC50/LEM3_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926:SF0">
        <property type="entry name" value="CDC50, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10926">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03381">
        <property type="entry name" value="CDC50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015840">
        <property type="entry name" value="DUF284_TM_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR015840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="270" mass="30908" checksum="A09F8609F31C4114" modified="2012-10-03" version="1">MSLLAYLSRIKGHLLSQKLPGTYRSESNRNFMAILFILGLINFTLGIMATLVYSSLMEVTIPYTNDMSTDVYLPKGRTYLFLEMKDFYQTNLRYSKSISYDQLRGERPKSLNSAKPLSYDDGKIIYPAGMLPNSFPHDSYRIDGVDIETEGISWESERNAIKPSSYTRDEVVSPPLWPDYDEIPNLSLDERFINWIYIAPFPSFRKLWGVVDVETEGMYTLNITSMFPYGKKYVSFAQSSIIGPKNYFLSIGLMLVGLAMILLSLHNHCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7AF33</accession>
    <name>I7AF33_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_070340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264782.1">
        <property type="nucleotide sequence ID" value="XM_009266507.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AF33"/>
    <dbReference type="GeneID" id="20521593"/>
    <dbReference type="KEGG" id="ero:EROM_070340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070340"/>
    <dbReference type="HOGENOM" id="CLU_089362_0_0_1"/>
    <dbReference type="OrthoDB" id="2191300at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="272" mass="30150" checksum="58BC627A8CB7EEE0" modified="2012-10-03" version="1">MHLSWFIKHTQGEDGWINLKMFQAWVHGSLVDPKHCKSKKNYLSCDFHDLKTMKFSTLKLATCYLYKVAVEAKISPKEEFAGESDSNYKNEYLITRMTGKDTFTPEMTRNMVLENGLAIVRETTTFVNPHEVKFVPGSVLESLDKVAFASDRFGTPNKFLTKPDFSYMEGYNKMSKQKNETKATETNSASSVDSTKSNSGKGEKGSTTSDDEKVSNGTEGGKTEDGSLENQDQNDQGNNITSDGETKTSDKSGFFPGGSFVRSSSGKYSSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFG1</accession>
    <name>I7AFG1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264942.1">
        <property type="nucleotide sequence ID" value="XM_009266667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFG1"/>
    <dbReference type="GeneID" id="20521759"/>
    <dbReference type="KEGG" id="ero:EROM_080230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080230"/>
    <dbReference type="HOGENOM" id="CLU_009923_1_0_1"/>
    <dbReference type="OrthoDB" id="273340at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056551">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040382">
        <property type="entry name" value="NOL10/Enp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14927:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23098">
        <property type="entry name" value="Beta-prop_NOL10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nucleolar protein 10-like N-terminal" evidence="1">
        <location>
            <begin position="6"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="33248" checksum="E2C4AF07E2E8DA31" modified="2012-10-03" version="1">MKFEKVSIVKDLGYTGSSIDLALSENRRYLVSVGVYKPSVKIYDLENVALKVERHLESDPIKVLPLAEDSSKICIIRNDRTMEFHARYGYHEGVKTPTLCFDGCLNKFRAEVMSGGKGPSIYRFNLDQGRFLRSYVVTMDEVCSIRMSECNGLIGVGGGKMVQFIDQRCREIVKTVEYNESPSSVDFSDNGIDFGVGTSEGIVYFHDLRARRALLTIKHDKKVKKILFNGKILVSMDKHGLKYCNKSGIVGEYPRDVEVNCFTCNGGVTFIGFDNGEIDEVISEDLGEVPQQYKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG13</accession>
    <name>I7AG13_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Mechanosensitive ion channel MscS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_090480</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265162.1">
        <property type="nucleotide sequence ID" value="XM_009266887.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG13"/>
    <dbReference type="GeneID" id="20521987"/>
    <dbReference type="KEGG" id="ero:EROM_090480"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090480"/>
    <dbReference type="HOGENOM" id="CLU_2196938_0_0_1"/>
    <dbReference type="OrthoDB" id="2190769at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="5">
        <location>
            <begin position="47"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="109" mass="12340" checksum="51529387B05951F2" modified="2012-10-03" version="1">MMVGKSILKFPFLSLRKNISESVVPRITISMAMLKPISVIMPKVLSDNLYKGAMALATCPFGVGDRIKIDKYDGVVKNISFWYVMLERGKGYVFIPTSHIYNTVIEMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>I7AG92</accession>
    <name>I7AG92_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar protein 56</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_091590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN83775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265272.1">
        <property type="nucleotide sequence ID" value="XM_009266997.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AG92"/>
    <dbReference type="GeneID" id="20564383"/>
    <dbReference type="KEGG" id="ero:EROM_091590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091590"/>
    <dbReference type="HOGENOM" id="CLU_015495_4_0_1"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
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        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
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        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="7">
        <location>
            <begin position="270"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="414"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="429"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="488" mass="56475" checksum="446B49402BF39C55" modified="2012-10-03" version="1">MEHLLFEYAGGYGLFELKEYEDMSKSTYDDYTKLTQVLNLKSRMKFNSFNEASDHLKCLSQGQIHEDLKKFLELNNVKILHCDFSLKKSLDELGIKQKESESIMRGVRRDFKKLMRLEEYDGKQTVLGLAYEYSREKVEYNVKREDFIVIHTVMLLEQVDKDINSYSMRIREIYGWVFPELGALLKENHEYISAVKYFVNEEVDSDYTKCDPGINEERLDEIKKLRKNSMGMKLSPVDMINLKKLIEIVDNKIKVRGSLGRYLKEKMSSIAPNLAAILGDVLGARIISQAGGLLNLAKAPASTFQLLGAEKSLFRSLKMKKRTPKYGLLYTSSYVSRVRDKDKARICRYIATKCSIAAKIDYFGESRGDEYGVELKSLIDKKIQSLCTNEEVERTSTAIQRVFDRINGISRDDIGKEDVQSKEISKKDTKPRKKPSRHTSKEEVFKFRERKSKDEGSSLKSNKRVSFDLSRNDVKETENISKRSNKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGT6</accession>
    <name>I7AGT6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_110220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265502.1">
        <property type="nucleotide sequence ID" value="XM_009267227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGT6"/>
    <dbReference type="GeneID" id="20564621"/>
    <dbReference type="KEGG" id="ero:EROM_110220"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110220"/>
    <dbReference type="HOGENOM" id="CLU_131615_0_0_1"/>
    <dbReference type="OrthoDB" id="2194244at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN84005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="174" mass="20503" checksum="8C2BB74F98C5DA96" modified="2012-10-03" version="1">MTYVSEEEALWVSKLDLEEESISRACFSFDIMRCKMCSEIFHAKWHEDHPCFSKDIDKQLNQRIEPITFAEGRLKDFSGDIQSYEYLKEALRIRPVVNKIWRLPHALLAEERKLWWKWIYGSSGKEDYYSDVSKKQWDVTHYGQSIDPAIGDIATSKRPANKAGECRTIYKRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>I7AH89</accession>
    <name>I7AH89_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_111690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265647.1">
        <property type="nucleotide sequence ID" value="XM_009267372.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AH89"/>
    <dbReference type="GeneID" id="20564768"/>
    <dbReference type="KEGG" id="ero:EROM_111690"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111690"/>
    <dbReference type="HOGENOM" id="CLU_109007_0_0_1"/>
    <dbReference type="OrthoDB" id="292213at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN84150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="217" mass="25223" checksum="BECB4798A19EC33C" modified="2012-10-03" version="1">MNPTKLLSTDNPLVYIGFVVSPVIGYIPQILSRDILLSPLISTFFIMSNILKVFHYSFERYSRFLLAQYVFAILLHMFLITINKRPLSTYEARILGNRTTKLLYRKYGVKGSVFGIVCIFVFFINLYGALYGTYEHCGRFSSALEITVNLLQLMLEREERNPENQKREPKRSPKEVYFCWVVGDMIKIWLMSSIEAPIIFIGTIVVQIFIDIFLIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7AL75</accession>
    <name>I7AL75_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263901.1">
        <property type="nucleotide sequence ID" value="XM_009265626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AL75"/>
    <dbReference type="GeneID" id="20520687"/>
    <dbReference type="KEGG" id="ero:EROM_010600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010600"/>
    <dbReference type="HOGENOM" id="CLU_1461758_0_0_1"/>
    <dbReference type="OrthoDB" id="2188976at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="183" mass="20783" checksum="D2D73BDFDB8EA0F0" modified="2012-10-03" version="1">MMQLGHLFAGQIVMYISFLLLVAVSESRIIPTGVNYLARSSFLRVSDAKGVFCFLFSFSTTIIGFMLETMRSTAKICKNDRMKYRVMKAQRGFTYMTLILFIDFLSAYIFKSLNDRTTSLLILGLASHICCSFTAFIGMDMLNTFFYLTFVISNLGVLLLTYVAGFDRAFIGVGHYLGRFRPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALG0</accession>
    <name>I7ALG0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sec1-like syntaxin-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_020340</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264006.1">
        <property type="nucleotide sequence ID" value="XM_009265731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALG0"/>
    <dbReference type="GeneID" id="20520792"/>
    <dbReference type="KEGG" id="ero:EROM_020340"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020340"/>
    <dbReference type="HOGENOM" id="CLU_009210_1_1_1"/>
    <dbReference type="OrthoDB" id="2228at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="493" mass="56572" checksum="C3DA5124396B1C06" modified="2012-10-03" version="1">MDILDLLKKKIIEDVFEVHLDAEWSVLVYDSYGASILKNFFVKSEFISHNIVISQRIEEHRERVDFPVIYFVRGDENVVEMINKDFGSRLYPSYAACVLSECQGLQPLVKVKRVDVDFVPFEQKVFKADPKQLYSVARTLNTVFNVSYSGSVCRGLGEMVEKMMGDEERKGELIILDRSIDLFTPLLHFFTFRTMLEDLEIENMEVFEKKYMGDKIWESVKNAHLGEVNSILRNHAHMLSKVAQKLDTKVDNKGLMKMVLEAPAAAKTKESLSKILALAQKCYDRFDYLSRFNEIEQLLATGYDKSGGKWSMKVDEVFEYLKSSGIERPDKIRILLLLKANGYQLQENEVGLLKSIGFKDEEIDVSFDNHENFIPLRGPGSNKYEVSRYEPVLANVLRSFIGKQKGHLQINRLTSSRIHLKSLRKSHLLSVKKDISYQKLYCVYITGGITFEEIRVAYEIGESLGVEIIVGSDNIITPRSYVEQLKSKCTRAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALN4</accession>
    <name>I7ALN4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FYR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_021090</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264081.1">
        <property type="nucleotide sequence ID" value="XM_009265806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALN4"/>
    <dbReference type="GeneID" id="20520871"/>
    <dbReference type="KEGG" id="ero:EROM_021090"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021090"/>
    <dbReference type="HOGENOM" id="CLU_112148_0_0_1"/>
    <dbReference type="OrthoDB" id="2192852at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003889">
        <property type="entry name" value="FYrich_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003888">
        <property type="entry name" value="FYrich_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05964">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51543">
        <property type="entry name" value="FYRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51542">
        <property type="entry name" value="FYRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="23041" checksum="13DB36EE16DC98AD" modified="2012-10-03" version="1">MEEEGKLAKRLVDAYNKRVELYMQRRSLEDSISAKLIDQRALREAIEMNKGLDRKEQAKPPDQGTMFGTGMHRLSLIDIGKLPTDNIDMFHTETAIYPVGYTCRKKYKKHNTYKRKAKDRILYICSVDPHKGLTISADDGRKWYGPTMWKDFVDSIEGTVEYKNVEEFFGFGNSALAKKIESLGDLSPFKKYIPLSRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALS1</accession>
    <name>I7ALS1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">WD40 domain-containing periodic tryptophan protein 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_021440</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264116.1">
        <property type="nucleotide sequence ID" value="XM_009265841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALS1"/>
    <dbReference type="GeneID" id="20520906"/>
    <dbReference type="KEGG" id="ero:EROM_021440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_021440"/>
    <dbReference type="HOGENOM" id="CLU_394919_0_0_1"/>
    <dbReference type="OrthoDB" id="407922at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051570">
        <property type="entry name" value="TBC1_cilium_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853">
        <property type="entry name" value="WD REPEAT CONTAINING PROTEIN 3 WDR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19853:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="446"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="705" mass="80835" checksum="1D6DF715C377321E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALY0</accession>
    <name>I7ALY0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="7">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_030330</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="7"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264151.1">
        <property type="nucleotide sequence ID" value="XM_009265876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALY0"/>
    <dbReference type="GeneID" id="20520944"/>
    <dbReference type="KEGG" id="ero:EROM_030330"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030330"/>
    <dbReference type="HOGENOM" id="CLU_000524_5_1_1"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
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    <keyword evidence="5 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="38"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="11">
        <location>
            <begin position="425"/>
            <end position="488"/>
        </location>
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        <location>
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            <end position="733"/>
        </location>
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    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="8">
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            <end position="959"/>
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        <location>
            <begin position="961"/>
            <end position="1044"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN82654.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1046" mass="118584" checksum="90DD3927BA659804" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM43</accession>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_031270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
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        </subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MIX-TYPE HOMEOBOX GENE 1-RELATED"/>
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    </dbReference>
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        </location>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="97" mass="11284" checksum="D9DB36F1BE1AF3E7" modified="2012-10-03" version="1">MQAKRRLIKNSGLSKAQKDFLNTYFEVNPNPNIQERAYIACQSLISEKKIRNWFQNRRARERGDCKASPCLSTFNGNASESYISKVHPTSNDLYIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AM71</accession>
    <name>I7AM71_ENCRO</name>
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        <submittedName>
            <fullName evidence="9">Transcription elongation factor S-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_040140</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
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        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Selman M."/>
                <person name="Burki F."/>
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                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
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            </authorList>
            <dbReference type="PubMed" id="22802648"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040140"/>
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    <dbReference type="OrthoDB" id="44867at2759"/>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="IP08861P-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS central" evidence="8">
        <location>
            <begin position="97"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS-type" evidence="7">
        <location>
            <begin position="211"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN82784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="252" mass="28710" checksum="B2AF889FD9207543" modified="2012-10-03" version="1">MIQATTLEEIKRLIKCNDLEALDSVLSTTRGITASDLEELIKLSDSKRIQALIRTRHLEIIKKSANEKEMGEEISSGSRDDADPSSALEEVATGESQKDRSIKMFFEAFKTSISDCSNRYAALLAKQITNEIFERNSADIAKLVRSKCLNLKDKNNPVLCRMVYDGEISPSRYVDMTSEEMKSESLRNEEVKMIEVSLYECQIPTQKAETDMFKCNRCGERKCSYRQLQTRSGDEPMTTFVTCECGNKWRFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMG3</accession>
    <name>I7AMG3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ski2 ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_041280</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264391.1">
        <property type="nucleotide sequence ID" value="XM_009266116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMG3"/>
    <dbReference type="GeneID" id="20521191"/>
    <dbReference type="KEGG" id="ero:EROM_041280"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041280"/>
    <dbReference type="HOGENOM" id="CLU_002902_0_0_1"/>
    <dbReference type="OrthoDB" id="64767at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055087">
        <property type="term" value="C:Ski complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070478">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000354">
        <property type="entry name" value="ATP-dependent RNA helicase SKI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012961">
        <property type="entry name" value="Ski2/MTR4_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08148">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01142">
        <property type="entry name" value="DSHCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="61"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="272"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="869" mass="99390" checksum="1325B0B4F489F0B6" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMH8</accession>
    <name>I7AMH8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Na+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_041500</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264411.1">
        <property type="nucleotide sequence ID" value="XM_009266136.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMH8"/>
    <dbReference type="GeneID" id="20521213"/>
    <dbReference type="KEGG" id="ero:EROM_041500"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041500"/>
    <dbReference type="HOGENOM" id="CLU_008635_5_0_1"/>
    <dbReference type="OrthoDB" id="2190219at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF1">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="190"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="343"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="6">
        <location>
            <begin position="18"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="421" mass="47531" checksum="0633563E519B7863" modified="2012-10-03" version="1">MNDVFRLLLASSSFVLLFGMISKYIKNNLFLSESLISVLVGIVLGHHGLNVFDTNYVYSKIMMYNFSRIVLCIQTMTVAMSLPKNYVVVKWQSIFFLVIVVGIIKCSFSFCLVYLFTNYNKSISWSLASCLTPTDPILSSSIIHGKLAMKMVPERIRLLLAAESGINDGMGIFLLFLGLDLLGHGLENGIYPFIINSILIRVLFSAVIGLSVGILSRQGLKYCYTRRLVGVESFLAHGIILAVFSMALMEFIGGSELICIFFAGIMFPFDEWFVLETKNSSLQEITDTILSTSFFLFFGSRIDFGRFTLSNVIVSLVIIFIRRPVICCLTYRQIPELRSRREALFVGWFGPIGVGALYYALLTDKIQETLTIDFVSCTVFLSVMIHGLTVPIYTYMKRESLNFNYEGEERSNVLWHEGWGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMI3</accession>
    <name>I7AMI3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264416.1">
        <property type="nucleotide sequence ID" value="XM_009266141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMI3"/>
    <dbReference type="GeneID" id="20521218"/>
    <dbReference type="KEGG" id="ero:EROM_041550"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041550"/>
    <dbReference type="HOGENOM" id="CLU_1107119_0_0_1"/>
    <dbReference type="OrthoDB" id="2190647at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="247" mass="28537" checksum="FAB854EF8C215EBB" modified="2012-10-03" version="1">MELVKGTDYIFCGSRKDIECTLRLPRPIIILTPYKSRCSALICWRDGNDIIMTPRDLSKNLDRMNGGHVVVENCELMEDFGYLPDLIDLRGKNISFILLNAQKAPRFAENPVLLSNSRHFIRAKGDERYAVIFALHKIYKNMWIVCKSVEKMNMFSKIFKLDLTVVKHGDDVKGKGVVVVMDELVNIECEELFYVGEECKGMRPLVLDMSKIGKFLYRIRDVCNMLSPAVIQGKRRLDINRLWNIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMP6</accession>
    <name>I7AMP6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lrg1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_050720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMP6"/>
    <dbReference type="GeneID" id="20521304"/>
    <dbReference type="KEGG" id="ero:EROM_050720"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050720"/>
    <dbReference type="HOGENOM" id="CLU_970090_0_0_1"/>
    <dbReference type="OrthoDB" id="20689at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00159">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963">
        <property type="entry name" value="RHO GTPASE ACTIVATING PROTEIN 18,19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14963:SF1">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN CONUNDRUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="2">
        <location>
            <begin position="67"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="287" mass="33622" checksum="9B49A9ECD8EF25E8" modified="2012-10-03" version="1">MERKSLGKPFERSLRTVNGLDDKDFEVYKGCFFDYYDDLIRKYFGAFEIFKLKQGRLARMFFSHDMLKLSSKYEWVVTRLSYGKEGELMVPRNFYILTETVLSLDCKVGGIFRVRNSITTIKECVETINMCVQRNLSYGEARRILIRDFNVIDLTTAFKEILREYSTTIIPESLIDTMFKIAKVESKEDQIILCQMLFISMPKPNRHVLEATIYFLYVIHDIATDKGKNHKDNMNMEGISTIMMPNLVIKPHSELDLEQVGVLVNFMKMFFENFTAIIHLDPNIMEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMQ9</accession>
    <name>I7AMQ9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">60S ribosomal protein L21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_050930</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266246.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMQ9"/>
    <dbReference type="GeneID" id="20521325"/>
    <dbReference type="KEGG" id="ero:EROM_050930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050930"/>
    <dbReference type="HOGENOM" id="CLU_103610_0_1_1"/>
    <dbReference type="OrthoDB" id="1539250at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN83024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="160" mass="18539" checksum="E1789768988737B1" modified="2012-10-03" version="1">MRSKGYRRGTRSLFSQDHRKHGVPHVSKYLEKYEIGDMVDILVNPAIMKGMPHKYYHGRTGRVYDVKPRSVNIALYKRVRGKYVIKKVIARIEHVRKSRCCEESQNRILKASELAKSAEARGMVLPPIKRKIEGPRKGMEISLKNNQPIEVGYEPHIVIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMT5</accession>
    <name>I7AMT5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uup ABC transporter ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_051230</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264551.1">
        <property type="nucleotide sequence ID" value="XM_009266276.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMT5"/>
    <dbReference type="GeneID" id="20521355"/>
    <dbReference type="KEGG" id="ero:EROM_051230"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051230"/>
    <dbReference type="HOGENOM" id="CLU_000604_36_6_1"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001092">
        <property type="entry name" value="ATP-binding cassette sub-family F member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000011">
        <property type="entry name" value="Putative ABC transporter ATP-binding component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF14">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="87"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="352"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="555" mass="62444" checksum="B8CD78BCFFC9487F" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMX2</accession>
    <name>I7AMX2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_060040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264596.1">
        <property type="nucleotide sequence ID" value="XM_009266321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMX2"/>
    <dbReference type="GeneID" id="20521401"/>
    <dbReference type="KEGG" id="ero:EROM_060040"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060040"/>
    <dbReference type="HOGENOM" id="CLU_030361_0_0_1"/>
    <dbReference type="OrthoDB" id="2186662at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046351">
        <property type="entry name" value="UTP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44163:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="620" mass="70123" checksum="5B1CCA900B378F7E" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7AN99</accession>
    <name>I7AN99_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GPN-loop GTPase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_061290</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Small GTPase required for proper localization of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264716.1">
        <property type="nucleotide sequence ID" value="XM_009266441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AN99"/>
    <dbReference type="GeneID" id="20521525"/>
    <dbReference type="KEGG" id="ero:EROM_061290"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061290"/>
    <dbReference type="HOGENOM" id="CLU_037460_0_2_1"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF3">
        <property type="entry name" value="GPN-LOOP GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="268" mass="30665" checksum="D0D63B5545AAE127" modified="2012-10-03" version="1">MRYAEVIIGPPGSGKSTYVMSKKAWLSHRNPYIINLDPGNCNDKEFDYSICSISPSLMYQERHNVGPNMSTKCILEEFYTRIDEFFQENIEGTDHYILLDFPGQVEFFISSDIPNSIIGYLKSNGYSVVVVNLVDLVFFSNDHSLISSYFISTLCLCLLESAQINVISKCDNWKKLGLKYSLEDIASLECLEEMEKKKKIHEEMASFVLNQGLLAYEVLDYDNPDSVLNLQVAIDKASGLFFEDDYFATEKITRISSKDDVFSRYRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANB7</accession>
    <name>I7ANB7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_061590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264746.1">
        <property type="nucleotide sequence ID" value="XM_009266471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANB7"/>
    <dbReference type="GeneID" id="20521556"/>
    <dbReference type="KEGG" id="ero:EROM_061590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061590"/>
    <dbReference type="HOGENOM" id="CLU_039747_0_0_1"/>
    <dbReference type="OrthoDB" id="2195422at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="520" mass="59906" checksum="4A6A4D616AC68872" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANV6</accession>
    <name>I7ANV6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exocyst complex component Sec6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_080540</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264971.1">
        <property type="nucleotide sequence ID" value="XM_009266696.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANV6"/>
    <dbReference type="GeneID" id="20521790"/>
    <dbReference type="KEGG" id="ero:EROM_080540"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080540"/>
    <dbReference type="HOGENOM" id="CLU_035820_0_0_1"/>
    <dbReference type="OrthoDB" id="2189254at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="583" mass="69647" checksum="4E0FE9A73A7CC30C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7APA8</accession>
    <name>I7APA8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proteasome regulatory subunit alpha-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_090320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265146.1">
        <property type="nucleotide sequence ID" value="XM_009266871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APA8"/>
    <dbReference type="GeneID" id="20521970"/>
    <dbReference type="KEGG" id="ero:EROM_090320"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090320"/>
    <dbReference type="HOGENOM" id="CLU_035750_0_0_1"/>
    <dbReference type="OrthoDB" id="40134at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="242" mass="27198" checksum="397A80D8CA856001" modified="2012-10-03" version="1">MSNLDFCTIYTTTGQIDQLTYAQKAADSGDTCIGMKSRHGVVLLAEKPKISPLYVAESDEKIRKAGNSVAVACTGLISDAFYVGYAIKDHVFHHKENFNEDPTPEIMKVYLNDIFHYFTRSISLRVLGVNSLTSVYKEGRFSLLHTDCSGKTLFYKAACIGRGTRRIKTELEKLDIDNMTIEEMVDAGVKVLYMAHDPSKDKEFDIEIGVVSTETGGKMRKLRNSEIQVFVDKYKHITVDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7APV9</accession>
    <name>I7APV9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_010020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263844.1">
        <property type="nucleotide sequence ID" value="XM_009265569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APV9"/>
    <dbReference type="GeneID" id="20520628"/>
    <dbReference type="KEGG" id="ero:EROM_010020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010020"/>
    <dbReference type="HOGENOM" id="CLU_1348916_0_0_1"/>
    <dbReference type="OrthoDB" id="2194333at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="CDD" id="cd01767">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="203" mass="23653" checksum="520A3628C16E37B9" modified="2012-10-03" version="1">MEERNKTEVSDGEVISYLVQSGFTKEEVQNAIKETGSKDIDLLVNFIISSTQDKIHKGVKQTKKEYNNTMPKEAAELEKKRREEIMKERIYREQLINQIKADMAEKLERDKLEDERLMSSTVEKSEDTSDCKIKLWLGDGTSSILGFSKDDTIEDLFKKIETRLNKKRFSLFKMNHVKPIERNTKKISEIPGLYPRGVLFIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7APX4</accession>
    <name>I7APX4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_010170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003518">
        <property type="protein sequence ID" value="AFN82362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009263859.1">
        <property type="nucleotide sequence ID" value="XM_009265584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7APX4"/>
    <dbReference type="GeneID" id="20520643"/>
    <dbReference type="KEGG" id="ero:EROM_010170"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_010170"/>
    <dbReference type="HOGENOM" id="CLU_1896187_0_0_1"/>
    <dbReference type="OrthoDB" id="2189046at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031504">
        <property type="entry name" value="DMAP1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17024">
        <property type="entry name" value="DMAP1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="135" mass="16397" checksum="95CDF571FC5D7CEA" modified="2012-10-03" version="1">MEKKDDDQGHEFSNDPVICRYLEILNSIESIRRDRDDVLSTHFNELSTYNINELEDRNAVIRRLINKEFTYPSAFLASSLGFNKTTWNRDYERTLREIGMYKRPKYSTLRNMLLFEKLKWLVIRYHEAKQKRKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQG3</accession>
    <name>I7AQG3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Splicing factor 3B subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_111380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265616.1">
        <property type="nucleotide sequence ID" value="XM_009267341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQG3"/>
    <dbReference type="GeneID" id="20564737"/>
    <dbReference type="KEGG" id="ero:EROM_111380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111380"/>
    <dbReference type="HOGENOM" id="CLU_055318_1_0_1"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003902">
        <property type="entry name" value="Tscrpt_reg_GCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50807">
        <property type="entry name" value="GCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GCM" evidence="2">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="327" mass="37788" checksum="96ED6E7556D8B972" modified="2012-10-03" version="1">MKSRAHMKIGIDDEYGFIHRIRPSRSSSNKRQRKREALQNRYEDLKLIVPHPELLEIEDSTCPDPIFHNEMKGRGGVPVPKHWKSSSGRMFPRSYYKPSYRIPRNVMKTGIPELRKMMKEREAGMSLRERIREKLYPKLGRSLVDQQVLYEAFFLEKGKPYLSNYGEFFEPGTDSFVKRCSPGVISNELMEALGIDDGAPPPWLFNMQKHGMPPSYPDAKIPGLNVPIPEGCSYGYQPLGWGEPLFEVSNGTTEKDTLQKDIGAIYNSENQYTRPVYIEDFEERVVINNNETTQDESPRAEVATKEDTEGKRKGRDRKEKLYKSIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQL2</accession>
    <name>I7AQL2_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Myb-like transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_111580</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265636.1">
        <property type="nucleotide sequence ID" value="XM_009267361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQL2"/>
    <dbReference type="GeneID" id="20564757"/>
    <dbReference type="KEGG" id="ero:EROM_111580"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111580"/>
    <dbReference type="HOGENOM" id="CLU_028567_14_2_1"/>
    <dbReference type="OrthoDB" id="2143914at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000010">
        <property type="entry name" value="Transcriptional activator Myb isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050560">
        <property type="entry name" value="MYB_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614:SF25">
        <property type="entry name" value="MYB PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45614">
        <property type="entry name" value="MYB PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="15"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="3">
        <location>
            <begin position="67"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="HTH myb-type" evidence="4">
        <location>
            <begin position="72"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN84139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="205" mass="23996" checksum="56EE06213A85E00A" modified="2012-10-03" version="1">MASLDQLSQNQKDTGIKLVKGPWRHEEDEKLLKLVKEFSPKNWSFIAKKLGSRVGKQCRERWHNHLNPQITKKPFTMEEEALIIELHSKFGNRWSEIAKYLPGRTDNAIKNYWNSSIQRRSEKVRRRSMFCSIDEFHKNQEIRYGKESSVLGISLQANMSQKTKRSSSVQDISEHVSNARSFDEDDFDEMDEIASQALLRICMSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQR1</accession>
    <name>I7AQR1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 7">2.7.7.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_030180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a central role as a glucosyl donor in cellular metabolic pathways.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDPGP type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 7"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264134.1">
        <property type="nucleotide sequence ID" value="XM_009265859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQR1"/>
    <dbReference type="GeneID" id="20520925"/>
    <dbReference type="KEGG" id="ero:EROM_030180"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030180"/>
    <dbReference type="HOGENOM" id="CLU_023632_3_0_1"/>
    <dbReference type="OrthoDB" id="932129at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00897">
        <property type="entry name" value="UGPase_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.10.10:FF:000001">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000002">
        <property type="entry name" value="UTP--glucose-1-phosphate uridylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002618">
        <property type="entry name" value="UDPGP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016267">
        <property type="entry name" value="UDPGP_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43511">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01704">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000806">
        <property type="entry name" value="UDPGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000806-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="492" mass="56078" checksum="57514469CFF16298" modified="2012-10-03" version="1">MQENKSKDATLSGDEDKDVHQFLDEFDDKCTCKLLKEMKETLEKIKKDHPNPVDLDEFYRLFERYLRTRNEKIVWERIRSPKDKIIQYSDIPEPTERSKELLKKLAILKLNGGLGTTMGCVGPKSAITVKDGKNFIDLIVKQVRHLNSKYNIDVPLILMNSFNTESMTDKIIFRYDRIRKFSQSKFPRISSETLLPVSPEHGDKGMYPPGHGDLFYSLKNSGMLEELLSEGYEYLFVSNIDNLASTVDLKLLEYFATNGLGFLMEVTDKTRADVKGGTLIEYKGGLRLLEIAQVPSSKKSEFTSFKKFTIFNTNNIWINLKDMKKKLEEGFFDLDIIENKKTLGDETVIQLETAIGSAIKYFPNSCGVVVPRSRFLPVKTCSDLFLVESNLFIEKNGTLQLHPSRVPKTCPIIKLIGKNFSKIENYEKCFKGIPDILELDILTVSGNVIFGKNVVLKGTVIILADEKSKICIPDRSELDDDILYGHLPILDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQU1</accession>
    <name>I7AQU1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CHK1-like Ser/Thr protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">EROM_030510</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264169.1">
        <property type="nucleotide sequence ID" value="XM_009265894.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQU1"/>
    <dbReference type="GeneID" id="20520962"/>
    <dbReference type="KEGG" id="ero:EROM_030510"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030510"/>
    <dbReference type="HOGENOM" id="CLU_000288_63_0_1"/>
    <dbReference type="OrthoDB" id="539158at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000571">
        <property type="entry name" value="Maternal embryonic leucine zipper kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF82">
        <property type="entry name" value="KP78A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="29"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="AFN82672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="224" mass="25358" checksum="BAF25D4A3A24B714" modified="2012-10-03" version="1">METVYYLRCFLLRLVVSNYPFLVSIMEKYELQETLASGSTAKIKKAVVVGNEKCAVKLISKGDMSLQMFLREAKIHGSLRHPNIVEFVDSYEDRERYCIVMKLGYGEVGNMIWSGVGLDPLLAHFYFRQLVSAVKYLHSKGVCHRDIKPENMLIDGNGNLLLSDFGFSTLFFHKGRRRRLRSFVGSLEYMAPEVFEGDYDGSLADIWSCGISLVVFLTGTLPVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQX5</accession>
    <name>I7AQX5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Flap endonuclease 1</fullName>
            <shortName evidence="16">FEN-1</shortName>
            <ecNumber evidence="16">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">EROM_030960</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with PCNA1 and PCNA2. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Nucleus</location>
            <location evidence="16">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Nucleus</location>
            <location evidence="16">Nucleoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Mitochondrion</location>
        </subcellularLocation>
        <text evidence="16">Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 16">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="16"/>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264214.1">
        <property type="nucleotide sequence ID" value="XM_009265939.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQX5"/>
    <dbReference type="GeneID" id="20521007"/>
    <dbReference type="KEGG" id="ero:EROM_030960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030960"/>
    <dbReference type="HOGENOM" id="CLU_032444_2_0_1"/>
    <dbReference type="OrthoDB" id="1937206at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000009">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 16" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 16" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2 16" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 16 19" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 16" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="1 16" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="18">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="17">
        <location>
            <begin position="145"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AFN82717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="345" mass="38970" checksum="DB2BA0E0A510ACC9" modified="2012-10-03" version="1">MGIKQLSKLLKENSVKGIKERPLAYYSSKRVAIDASMSIYQFLIAVRSDGSTLGNGDTTTSHLVGLFYRTIRMVELGITPVYVFDGVPPEVKMKELGKRNERRATADKEYKDASEAGDKRLMEMYDKRKTKVTGIHVEECKRLLGLMGIPFETAPSEAEAYCALLCKKRAVYGVATEDMDSLTFGSPVVLRNFSGTRSKKLPVVEYNLRQLLEDLSLDQDEFIDLCILLGCDYCDTLKGIGPKKALGLIKKHRSIERILQEENLETPDEWRYEDAQKIFRNLAEIGDAKEFNISWKSIDRNGIVKFLVEEKGFDLERVNKGVDKLINSGRKSTQGRLDGFIAKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR39</accession>
    <name>I7AR39_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">MdlB ABC transporter ATPase/permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_040440</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264309.1">
        <property type="nucleotide sequence ID" value="XM_009266034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR39"/>
    <dbReference type="GeneID" id="20521108"/>
    <dbReference type="KEGG" id="ero:EROM_040440"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040440"/>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_1"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034040">
        <property type="term" value="F:ATPase-coupled lipid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF654">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="37"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="345"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN82812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="557" mass="63170" checksum="710AC4535AD9946C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7AR52</accession>
    <name>I7AR52_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">triacylglycerol lipase</fullName>
            <ecNumber evidence="6">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Autophagy-related protein 15</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">EROM_040590</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Binds to both phosphatidylinositol (PI) and phosphatidylinositol 3,5-bisphosphate (PIP2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome</location>
            <location evidence="3">Multivesicular body membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Prevacuolar compartment membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="6"/>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264324.1">
        <property type="nucleotide sequence ID" value="XM_009266049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AR52"/>
    <dbReference type="GeneID" id="20521123"/>
    <dbReference type="KEGG" id="ero:EROM_040590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040590"/>
    <dbReference type="HOGENOM" id="CLU_081902_0_0_1"/>
    <dbReference type="OrthoDB" id="58570at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004620">
        <property type="term" value="F:phospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034496">
        <property type="term" value="P:multivesicular body membrane disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046461">
        <property type="term" value="P:neutral lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006660">
        <property type="term" value="P:phosphatidylserine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050805">
        <property type="entry name" value="ATG15_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47175:SF2">
        <property type="entry name" value="LIPASE ATG15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="AFN82827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="296" mass="33347" checksum="0540DB9CD3DBF21C" modified="2012-10-03" version="1">MLFHFLQASLYNNTHETIHNAFYPSGRILESGTASSIDAFQEYDLNLIDRVRLMELVEMASNTYHSHDLDTILDKFGYDGDGVRAKAFRYNGRVAIAFKGTTLSVMGVGIGRTSKKDKLLDRILYSICKDKGCEEEKIREFDSIGYFSDGLKIVDYVKRTYHEDKPILIGHSLGGTIASLLGIRYNLSAVAFSSPGDAHIASILGFYDTKRDHSNILHIGMCNDVVFRGECSKPYSPCNILGYAIETRCHVGRSLCIKDGEWNSLIYHPIDVMRAKIMMPEELILVEDNTRTNCVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARE3</accession>
    <name>I7ARE3_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_050080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264439.1">
        <property type="nucleotide sequence ID" value="XM_009266164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARE3"/>
    <dbReference type="GeneID" id="20521241"/>
    <dbReference type="KEGG" id="ero:EROM_050080"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050080"/>
    <dbReference type="HOGENOM" id="CLU_834266_0_0_1"/>
    <dbReference type="OrthoDB" id="2193169at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003707599" evidence="1">
        <location>
            <begin position="17"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="325" mass="35644" checksum="F343408F94AFF6F3" modified="2012-10-03" version="1">MRLFLITLSATHFVDATLSRTHASHHGTHHIHHPRVSNLRRYHSFFHHSHGKPLGGMGIIGARRISPVYLPGNSSPLVADVGNDAGESGVTLAPSVTNGSEERSVGIYISPSRSGKTTSGRDRLISLHHLIHHAQGEALKAKAISSAAKLQTSALLAQKQELNAKIMANKAEMLKEEEQRLVAQRSADPLILVKDTVPIANVANNNFSFEHHNELLSKKLDMIKKDQDDAVRKAENIARLHKARTRILPDSLVSSDPRQFKDFVNSGPIKDLTFEIPVDPSVGNYYISGNPGEDLQSSEAKLTTGDLPYFLAHSDGLDLNQSYHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARL9</accession>
    <name>I7ARL9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein transport Sec61-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">EROM_050910</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264519.1">
        <property type="nucleotide sequence ID" value="XM_009266244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARL9"/>
    <dbReference type="GeneID" id="20521323"/>
    <dbReference type="KEGG" id="ero:EROM_050910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050910"/>
    <dbReference type="HOGENOM" id="CLU_167752_2_1_1"/>
    <dbReference type="OrthoDB" id="2194864at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12309">
        <property type="entry name" value="SEC61 GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="AFN83022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="72" mass="7969" checksum="4CD0A5553B4EE013" modified="2012-10-03" version="1">MSQKSRSPSLLADYLKSVRLFTKKCVKPSGKEFAMSVKRHAIGIGFLGILGYAIKLIHIPINNIIVSNPNKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARN7</accession>
    <name>I7ARN7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">26S proteasome regulatory complex component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_051110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264539.1">
        <property type="nucleotide sequence ID" value="XM_009266264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARN7"/>
    <dbReference type="GeneID" id="20521343"/>
    <dbReference type="KEGG" id="ero:EROM_051110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051110"/>
    <dbReference type="HOGENOM" id="CLU_055005_0_0_1"/>
    <dbReference type="OrthoDB" id="268763at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="1">
        <location>
            <begin position="286"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="387" mass="45484" checksum="7B410507042F1F7C" modified="2012-10-03" version="1">MEELLEKERRARVRNDYEALLKIFEDLMGLCRSDEELAGLARVLTIKRGQNRMAIKWMISQLFERKKKEQGFVGFFCLILRDVIEGRIFLEEERIYITKELKERYESSGDIKSALDVIINVPVETFTMVKESVVVNYQLEQLRLCVKNHDWIRADITMKKIRRKYFEESGNVEEKTKFYELIVLLHLGQRNYFNASDVYYALSKLGKNSTGYVVLSSFFCILTTCETEMEDVVCKRIDMLKKLSEDKNNDEVVRSIVNRFLSRIVLDKSMANEIQQAFSSVLDVSVYLNDLVSAIDEHNFRIVERFYSSITIQEISLVMQSPVEDIIKKISFMVNNGFTRCKINQKTEIVEFGKRKWNESVDDVMNKLIKCNHLIHKERLKFSIKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ARZ5</accession>
    <name>I7ARZ5_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_060380</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264629.1">
        <property type="nucleotide sequence ID" value="XM_009266354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ARZ5"/>
    <dbReference type="GeneID" id="20521435"/>
    <dbReference type="KEGG" id="ero:EROM_060380"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060380"/>
    <dbReference type="HOGENOM" id="CLU_554422_0_0_1"/>
    <dbReference type="OrthoDB" id="2194683at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011400">
        <property type="entry name" value="EIF3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 EIF3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14068:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="502" mass="57828" checksum="6D23AB302BA1AF4C" modified="2012-10-03" version="1">MNNQILIIGLDQEKNSLPEITPDNSLFRDGLCIVQLKTSQQAGLLLRKLENFSHVKAYSLKDFYDTMSSEDKEYVPLKKLEPREFVDFQFSGREQFVILNNGILSLFEQKNKEFEEISGFNDVRKCMVSNDGVFVCFLVDSTIEIRVGRRLEVFSKIKLNDEVVDVSFSPDNKFIVVFTRKETSVWSTFKNECLVRQQGGKGEIAFDEDCVYFLDKGKAFSLSLGKEVALDQKLSNVKFIKYHNDQKVEFSSDRVQRIRYTKGEHKLSKSHANIEEIKFHFSENRCFALITKNIQKKNVQFVESYGMDGITLTQLEGCVEQMEVSDKMFAIVDSKQTLLLYSRDKFGFNMIKQIKKEDDLFIALYDEICCVHDSDTGNIEFYDNGELRSAYSHPSCTSILWSHSGLYAASASAGDCSSGLVQMFNRNGKLLWKKVFNRLSLLMWRPFIRIPEEDKAKAIEQFEGTVTDEVSSSEEHTVDVDELLSKWKSYLISRKQRVLSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>I7AS54</accession>
    <name>I7AS54_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin</fullName>
            <ecNumber evidence="6">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="6"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264724.1">
        <property type="nucleotide sequence ID" value="XM_009266449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AS54"/>
    <dbReference type="GeneID" id="20521533"/>
    <dbReference type="KEGG" id="ero:EROM_061360"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061360"/>
    <dbReference type="HOGENOM" id="CLU_065681_4_2_1"/>
    <dbReference type="OrthoDB" id="186462at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF75">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0409">Iron storage</keyword>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="7">
        <location>
            <begin position="11"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="174" mass="19779" checksum="C9A5D5E4D874003A" modified="2012-10-03" version="1">MDPESNQALTDEWKAEAGLLLSSQLLLEYNAFYFYAACAAHFGNPNVCLKGLERFFKESSLDENEHAQRIINFMNMRSLNIEFRTIEAPDIKRYEKTSDILKASKEFEQTVLNNILTVSEIAAKAGDGSIVEFFEDFIAEQVKSISEFNDLYVNCVRCGDDGMGLFLFDQSLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Formin-like protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">EROM_071160</name>
    </gene>
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        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266589.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ASF9"/>
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    <dbReference type="KEGG" id="ero:EROM_071160"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071160"/>
    <dbReference type="HOGENOM" id="CLU_025884_0_0_1"/>
    <dbReference type="OrthoDB" id="2196228at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
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        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051425">
        <property type="entry name" value="Formin_Homology"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725:SF1">
        <property type="entry name" value="DISHEVELLED ASSOCIATED ACTIVATOR OF MORPHOGENESIS, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45725">
        <property type="entry name" value="FORMIN HOMOLOGY 2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="1">
        <location>
            <begin position="329"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="680" mass="78773" checksum="CD2ADD768EADAFD3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATA0</accession>
    <name>I7ATA0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Telomerase reverse transcriptase</fullName>
            <ecNumber evidence="2 13">2.7.7.49</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Telomerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">EROM_090300</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.49"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Chromosome</location>
            <location evidence="13">Telomere</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the reverse transcriptase family. Telomerase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.49" evidence="2 13"/>
    <dbReference type="EMBL" id="CP003526">
        <property type="protein sequence ID" value="AFN83647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265144.1">
        <property type="nucleotide sequence ID" value="XM_009266869.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATA0"/>
    <dbReference type="GeneID" id="20521968"/>
    <dbReference type="KEGG" id="ero:EROM_090300"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090300"/>
    <dbReference type="HOGENOM" id="CLU_018541_0_0_1"/>
    <dbReference type="OrthoDB" id="289721at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXa"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000333">
        <property type="term" value="C:telomerase catalytic core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003720">
        <property type="term" value="F:telomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070034">
        <property type="term" value="F:telomerase RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01648">
        <property type="entry name" value="TERT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021891">
        <property type="entry name" value="Telomerase_RBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003545">
        <property type="entry name" value="Telomerase_RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12066:SF0">
        <property type="entry name" value="TELOMERASE REVERSE TRANSCRIPTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12009">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01365">
        <property type="entry name" value="TELOMERASERT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00975">
        <property type="entry name" value="Telomerase_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 15" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="9 13" id="KW-0779">Telomere</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="14">
        <location>
            <begin position="368"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="AFN83647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="812" mass="94580" checksum="C2F7B7048C10A1F0" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATL2</accession>
    <name>I7ATL2_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Chromatin-remodeling ATPase INO80</fullName>
            <ecNumber evidence="14">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">EROM_091930</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">ATPase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of the INO80 chromatin-remodeling complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The DBINO region is involved in binding to DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="14"/>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265304.1">
        <property type="nucleotide sequence ID" value="XM_009267029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATL2"/>
    <dbReference type="GeneID" id="20564417"/>
    <dbReference type="KEGG" id="ero:EROM_091930"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091930"/>
    <dbReference type="HOGENOM" id="CLU_000315_20_1_1"/>
    <dbReference type="OrthoDB" id="372624at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000005">
        <property type="entry name" value="Photoperiod-independent early flowering 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020838">
        <property type="entry name" value="DBINO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050520">
        <property type="entry name" value="INO80/SWR1_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685:SF2">
        <property type="entry name" value="CHROMATIN-REMODELING ATPASE INO80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45685">
        <property type="entry name" value="HELICASE SRCAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13892">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51413">
        <property type="entry name" value="DBINO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DBINO" evidence="18">
        <location>
            <begin position="82"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="286"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="730"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AFN83807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="883" mass="102614" checksum="DD0E9E86DF3FB0A3" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATS5</accession>
    <name>I7ATS5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein MAK16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_100710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MAK16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265384.1">
        <property type="nucleotide sequence ID" value="XM_009267109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATS5"/>
    <dbReference type="GeneID" id="20564501"/>
    <dbReference type="KEGG" id="ero:EROM_100710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100710"/>
    <dbReference type="HOGENOM" id="CLU_050888_2_1_1"/>
    <dbReference type="OrthoDB" id="10251342at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006958">
        <property type="entry name" value="Mak16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405">
        <property type="entry name" value="MAINTENANCE OF KILLER 16 MAK16 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23405:SF4">
        <property type="entry name" value="PROTEIN MAK16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04874">
        <property type="entry name" value="Mak16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003352">
        <property type="entry name" value="MAK16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="5">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR003352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="232" mass="27646" checksum="9BDB3649452A95DF" modified="2012-10-03" version="1">MSDESIWRNIGGDGYCSFKMKTEEDTLCRNQNNVTGICNRFSCPLANSKYATVRAVGEELSLFVKEPERVHIPRDAYEEIKLSSNYEEALKQIEENLEFWDEKIIHKCKQKLSKLTQYLRRLEYFKEHGRTEYMVRKKRMNRREKLRALKTLNKINFEKNIGDELMLRLESGIYGTELKDRFHLANEAAVEREKKQNRKKRYVADIEESDTMFEAEPRDIKKKAKKKATMKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATT1</accession>
    <name>I7ATT1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_100760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265389.1">
        <property type="nucleotide sequence ID" value="XM_009267114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATT1"/>
    <dbReference type="GeneID" id="20564506"/>
    <dbReference type="KEGG" id="ero:EROM_100760"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100760"/>
    <dbReference type="HOGENOM" id="CLU_084270_0_0_1"/>
    <dbReference type="OrthoDB" id="2193149at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="35056" checksum="22D7124A3E43C9F7" modified="2012-10-03" version="1">MEGVRFADVLQSFEKKTEDDEYVSVERDYNERRVIENDVRRTELLGVDKKDYKMVKFLKKLLFVELDKIPIRYTQGMSEVASVFVLYYFQEIVGEEIAKGILGGGDPESPGEGSPENFIDVPEDENVELKRFLSRHKDTTAKLGVVLTNVFKRKLEPLVTDDFKIYKENMKIFVEMMKKRGIKIPELESYKFMGSILTFFLRNLSKIEDVHKVFEIILSCPNTCPFLLLVQFYGRIANNKPIENINNNLFTKVIRLEEEFVETKKRIERKRPEFSKRKIIAVGGVISIVTAIVVYKIARKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AU02</accession>
    <name>I7AU02_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">40S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_101470</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265459.1">
        <property type="nucleotide sequence ID" value="XM_009267184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AU02"/>
    <dbReference type="GeneID" id="20564577"/>
    <dbReference type="KEGG" id="ero:EROM_101470"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_101470"/>
    <dbReference type="HOGENOM" id="CLU_010412_5_4_1"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN83962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="59" mass="6854" checksum="1F6A1E8776488FCF" modified="2012-10-03" version="1">MATITINKAGKVRNQTPKDPVVEKERKKCGRCRQRLKFEKRNDMGYFEVAGKMKLNPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I7AUC8</accession>
    <name>I7AUC8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">WD40 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_111050</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265584.1">
        <property type="nucleotide sequence ID" value="XM_009267309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AUC8"/>
    <dbReference type="GeneID" id="20564704"/>
    <dbReference type="KEGG" id="ero:EROM_111050"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111050"/>
    <dbReference type="HOGENOM" id="CLU_612532_0_0_1"/>
    <dbReference type="OrthoDB" id="431715at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018983">
        <property type="entry name" value="U3_snoRNA-assocProt_15_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924:SF26">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19924">
        <property type="entry name" value="UTP15 U3 SMALL NUCLEOLAR RNA-ASSOCIATED PROTEIN 15 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09384">
        <property type="entry name" value="UTP15_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="U3 small nucleolar RNA-associated protein 15 C-terminal" evidence="7">
        <location>
            <begin position="318"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN84087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="447" mass="51162" checksum="93F4486248FAE998" modified="2012-10-03" version="1">MVYFRSKNFGIADSSRESFDTVYEQEGPITSLCCCKDGIVVGCKNRAVVIKKGENKKKKIVKFDRDISAIDAWEDLTAYGDEGGEIKVVGRMRTIVRHYQEHEARVNEIKINEDMLISCSDDMKIKIFELSRAESVATIAENTDYVKSIDVGDGMIFSGSYDGFINGYSLSTFKRVFEHNTKRRVSKVRILGGGRVAFASGNEVCVIDVENGGKEIGKAFHIKEVTSMVFYAGRLYTVSLDANVRVFTTDLRMISKIGVQGGILSISIFDDVLCLGLEDREVLELAKEQEKKDVMELRYRGNELEDEAKIDVVPNPLVKFSFLERRLNAFEYKKSMIHAMEKRDIQKVFAVMNYIHEKQKFEHALQDLNRREVERILDVIIEFFTVRELVPVFSECIETILSLYERDIRDDSMLSRKIEVLGAAIDDELYFQEENLRSIAFLECFRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI18</accession>
    <name>I6ZI18_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription initiation factor TFIID subunit 13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">EROM_040910</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TAF13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264355.1">
        <property type="nucleotide sequence ID" value="XM_009266080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZI18"/>
    <dbReference type="GeneID" id="20521155"/>
    <dbReference type="KEGG" id="ero:EROM_040910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040910"/>
    <dbReference type="HOGENOM" id="CLU_076665_4_1_1"/>
    <dbReference type="OrthoDB" id="10266074at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07978">
        <property type="entry name" value="HFD_TAF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003195">
        <property type="entry name" value="TFIID_TAF13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380:SF5">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11380">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID/SUPT3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02269">
        <property type="entry name" value="TFIID-18kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="AFN82858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="103" mass="12258" checksum="C7ECE332D6C21393" modified="2012-10-03" version="1">MRESRKVSFMKDIKVMMYGYGDVIAPRYDTAEALHGYVLDYMSILLTNTHNMAQVKGKTKTEDLLYYLKRDRKKYSRVRHLLITNEEIKLAKKAFDPKDYEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZI22</accession>
    <name>I6ZI22_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Myosin heavy chain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">EROM_040960</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
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        <property type="protein sequence ID" value="AFN82863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266085.1"/>
    </dbReference>
    <dbReference type="GeneID" id="20521160"/>
    <dbReference type="KEGG" id="ero:EROM_040960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_040960"/>
    <dbReference type="HOGENOM" id="CLU_000192_7_14_1"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MYSc_class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.360">
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
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    </dbReference>
    <dbReference type="InterPro" id="IPR008989">
        <property type="entry name" value="Myosin_S1_N"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004009">
        <property type="entry name" value="SH3_Myosin"/>
    </dbReference>
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        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF857">
        <property type="entry name" value="MYOSIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02736">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SH3_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="5 8" id="KW-0518">Myosin</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="31"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Myosin motor" evidence="11">
        <location>
            <begin position="83"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="8">
        <location>
            <begin position="624"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1119"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="825"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1349"/>
            <end position="1397"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1484"/>
            <end position="1518"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="1588"/>
            <end position="1671"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="176"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51844"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="AFN82863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1679" mass="197571" checksum="5BF4FC519C22EB89" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZJ74</accession>
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        <submittedName>
            <fullName evidence="3">Polyadenylate-binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_070270</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83278.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_009266500.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="20521586"/>
    <dbReference type="KEGG" id="ero:EROM_070270"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070270"/>
    <dbReference type="HOGENOM" id="CLU_020551_3_1_1"/>
    <dbReference type="OrthoDB" id="5411533at2759"/>
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    </dbReference>
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        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PUF60_regulator"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60-B-RELATED"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="58"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="142"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="251"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="333" mass="37649" checksum="17FBE9F2368E9F40" modified="2012-10-03" version="1">MIVVNDEDLKKTIEEVANAKRNELKNLDAVYKSVIEYGKWVIPKEGIKSVKFDSTLLNKVYIGNITASKGSIKDLLSVFGPVSVTYISGKECAFADFDNPHAAYLCMEEINGMAHDGKILKAGRTSTFPSEIPKDLGPPDETIVYVSNIDLDIEEEQLKDVFGKMGEIKDVRLVYEESFVHKGYGYVQFSRPGDARRALGYSNKISLYGKRIRIGPSVIKMDLPKRSHFAVPEQVFEIKKRIENVIFGKGKMVVLRNLIDIDDADDDFDQEIENEMRKYGNVERFGISKEGEVVVYCLYSTEDEAKHSFNILNGRFFGGRRIRAEMSCGDFSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZKC1</accession>
    <name>I6ZKC1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">EROM_091020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
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            <taxon>Unikaryonidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
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                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265215.1">
        <property type="nucleotide sequence ID" value="XM_009266940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZKC1"/>
    <dbReference type="GeneID" id="20564326"/>
    <dbReference type="KEGG" id="ero:EROM_091020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091020"/>
    <dbReference type="HOGENOM" id="CLU_2427029_0_0_1"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RING"/>
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    </dbReference>
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        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="AFN83718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="91" mass="10552" checksum="EDBB852F48693750" modified="2012-10-03" version="1">MNLCRVCDCEISIRHGTPCGHSFCYLCIKRHLSGHDTCPVCGRRPLSMKDLVGLRSSAKKWDSRIRMGEDPKILRVLYRLKRYIKEDRESG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZL04</accession>
    <name>I6ZL04_ENCRO</name>
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        <recommendedName>
            <fullName evidence="11">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="11">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">EROM_110190</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="11"/>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265500.1">
        <property type="nucleotide sequence ID" value="XM_009267225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZL04"/>
    <dbReference type="GeneID" id="20564618"/>
    <dbReference type="KEGG" id="ero:EROM_110190"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110190"/>
    <dbReference type="HOGENOM" id="CLU_000524_5_1_1"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
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        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
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        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
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        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 19" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="15">
        <location>
            <begin position="18"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="14">
        <location>
            <begin position="175"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="423"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="523"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="628"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="12">
        <location>
            <begin position="675"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="13">
        <location>
            <begin position="1025"/>
            <end position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="AFN84003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="1110" mass="126215" checksum="53C4DFF29B313D17" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZTJ4</accession>
    <name>I6ZTJ4_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_050530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264483.1">
        <property type="nucleotide sequence ID" value="XM_009266208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZTJ4"/>
    <dbReference type="GeneID" id="20521285"/>
    <dbReference type="KEGG" id="ero:EROM_050530"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050530"/>
    <dbReference type="HOGENOM" id="CLU_2003897_0_0_1"/>
    <dbReference type="OrthoDB" id="2190085at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="14325" checksum="195783504B6FF469" modified="2012-10-03" version="1">MKSYMKDGLRIGFTKEASTDNIETISLEIARKFELLSGICDNRSLSEFPVSIIKEVVGLCNDLKDLTPHYGMPDEYVNYVRENRDVLGYFERSESDVKSEETRIINKRKTFDAFRSMVEKIGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZU95</accession>
    <name>I6ZU95_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Polyphosphate synthetase Vtc4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_061400</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264728.1">
        <property type="nucleotide sequence ID" value="XM_009266453.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZU95"/>
    <dbReference type="GeneID" id="20521537"/>
    <dbReference type="KEGG" id="ero:EROM_061400"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_061400"/>
    <dbReference type="HOGENOM" id="CLU_009308_1_0_1"/>
    <dbReference type="OrthoDB" id="2243669at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07751">
        <property type="entry name" value="PolyPPase_VTC4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="519"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="546"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="584"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="7">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="611" mass="72047" checksum="B9DF81000C464F2C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZUB9</accession>
    <name>I6ZUB9_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_070100</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264758.1">
        <property type="nucleotide sequence ID" value="XM_009266483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZUB9"/>
    <dbReference type="GeneID" id="20521568"/>
    <dbReference type="KEGG" id="ero:EROM_070100"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_070100"/>
    <dbReference type="HOGENOM" id="CLU_2291685_0_0_1"/>
    <dbReference type="OrthoDB" id="2191932at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="101" mass="11793" checksum="BEF627A66678495F" modified="2012-10-03" version="1">MDVFTSNCKENLTVGQDNHIKRNALQTTAIGSVFCGRLERKEQYLYEGSISVGRVKLLKDFYEKSVFLSKNIKIRKTFYDLNPSVKERVKMLENMIVKQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZVK9</accession>
    <name>I6ZVK9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Threonylcarbamoyl-AMP synthase</fullName>
            <shortName evidence="13">TC-AMP synthase</shortName>
            <ecNumber evidence="3 13">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">EROM_091600</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3 13"/>
    <dbReference type="EMBL" id="CP003528">
        <property type="protein sequence ID" value="AFN83776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265273.1">
        <property type="nucleotide sequence ID" value="XM_009266998.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZVK9"/>
    <dbReference type="GeneID" id="20564384"/>
    <dbReference type="KEGG" id="ero:EROM_091600"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_091600"/>
    <dbReference type="HOGENOM" id="CLU_031397_0_1_1"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXc"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010923">
        <property type="entry name" value="T(6)A37_SUA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004930">
        <property type="entry name" value="Tln_factor_SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="15">
        <location>
            <begin position="5"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>L-threonine</name>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR004930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR004930-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="AFN83776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="337" mass="37149" checksum="8F64ED599B34D7BD" modified="2012-10-03" version="1">MRIVSIEELDVNSIVKFFDDVVVIPTETVYGLAASIHNPRAVERIFELKRRPADNPLIVHVSSEQMLEEIVEGPIPVEYRKLMDRFWPGPLSLLFKSKDSVSPVIRGGLSTIAVRMPKNKQLLEIIERLGVPVAAPSANVSGRPSPTTVDHVIEDFGDKVEMIIDGGPCSVGVESTVVWYADGVLQILRPGGVGIEDIKDVVDCEVVMKNKIEGGEEVLSPGQKYKHYSPRSPLVLFKGRPKEMESGILKYIEANPGIARIGVGLHSGINIETPLGRKVDIFNMGNVKKEVCRNLFEGLRKLDKTSDVILVAGIDCNEEGYAIMDRLERAADEIIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWD7</accession>
    <name>I6ZWD7_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_110990</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265578.1">
        <property type="nucleotide sequence ID" value="XM_009267303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWD7"/>
    <dbReference type="GeneID" id="20564698"/>
    <dbReference type="KEGG" id="ero:EROM_110990"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_110990"/>
    <dbReference type="HOGENOM" id="CLU_644099_0_0_1"/>
    <dbReference type="OrthoDB" id="2196354at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="276"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="375"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN84081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="423" mass="48516" checksum="7878EC2725488620" modified="2012-10-03" version="1">MGLFTDENEKKPLPASENFQDNTLRSPNTSQQLGKYIVNGLEMLSLSDIKVEVDRKNEAFDDGISDDHSEGLSMFGTISAESNGNEDCVYLSSIYKTYMKVNEKIDKLIRREYFDRTFVKCVLEEICRGDLEKARGTICKELKDRDPHFSVETYCSEQRICSGGNVSQSINGLLGDLRDIEEISHKLRRMFFDMRSKSFEAELGYARSSSEVVKQLDKCSRELEDLKRINGMIAKDVVGAIKDFGIDTSVKENDFYDVSKALKDVLKGIRKRVKTMESMSQEVEMKEKEYENVLKDYTELKGKMDRIINENASFSEAVARLSTKNAKIRKDYDTLKEALKKTVESNKKKSITIDRQKKIIDVLQSRIGDECILPVNELQSKIDEIRRRADSELDLSIKSKLEEEIADYERRMSDFLSLLNKTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>I6ZWL6</accession>
    <name>I6ZWL6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">U3 small nucleolar ribonucleoprotein protein IMP4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_111800</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003530">
        <property type="protein sequence ID" value="AFN84161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265658.1">
        <property type="nucleotide sequence ID" value="XM_009267383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I6ZWL6"/>
    <dbReference type="GeneID" id="20564779"/>
    <dbReference type="KEGG" id="ero:EROM_111800"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_111800"/>
    <dbReference type="HOGENOM" id="CLU_040063_2_1_1"/>
    <dbReference type="OrthoDB" id="10253204at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome XI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042134">
        <property type="term" value="F:rRNA primary transcript binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044281">
        <property type="entry name" value="IMP4/RPF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22734:SF2">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04427">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="110"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN84161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="310" mass="36323" checksum="122B41E97FAF07EB" modified="2012-10-03" version="1">MRLRRLIPARYTVNKALLHTFLLDFLRILSCRFLLLGSPYSMNRRKRERKEYLLRKERERLDKEMEEKKQRLGEALETNTKIPHGIRKDAATLLNEMIYEGKIEEEHRYPKVMVTTSRSPSSQLLHFSKQLSLVLNGENFIRGQLCEEEISDVAHKHGYTCVVIVHENRGRPASLAISYFPFGPTMRFTIIDHFLTRRTFPLSPKVYFICDNMEGPVGIKVKERIALLFPKCSGAKRIVSLVNRNDTIAFRHYLIEKGDKTVLNKSLGMDLRVYEIRKGTFEVDGEFEWAYKPYMNSRRTREEIGCTSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADJ4</accession>
    <name>I7ADJ4_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PSP1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264127.1">
        <property type="nucleotide sequence ID" value="XM_009265852.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADJ4"/>
    <dbReference type="GeneID" id="20520918"/>
    <dbReference type="KEGG" id="ero:EROM_030110"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030110"/>
    <dbReference type="HOGENOM" id="CLU_080493_1_0_1"/>
    <dbReference type="OrthoDB" id="243127at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047767">
        <property type="entry name" value="PSP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007557">
        <property type="entry name" value="PSP1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041131">
        <property type="entry name" value="RicT_YaaT_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43830:SF3">
        <property type="entry name" value="PROTEIN PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04468">
        <property type="entry name" value="PSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51411">
        <property type="entry name" value="PSP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP1 C-terminal" evidence="1">
        <location>
            <begin position="145"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="244" mass="29020" checksum="7B340568433E7728" modified="2012-10-03" version="1">MSFCKNWASPFLNSLWSETSETWQENQEDWRIVGTNPFKRRSENACKIERRITDIPSKHVADNIENSFRFMSITPETHYYIIQFESKRLDVAFVDVNINVKKNSYVILEADRGEDCGIVMGVTTKEKLEKLLKKYDDISNEVQPKRIYRLATLLDLEALERKRSMQARALEFCRERAMLGGLDMEVVGCEYQWDLNKITFYFASDERVDFRELVKELYRVYKTRIWMCAIEKSKNKYLKELIEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>I7ADK7</accession>
    <name>I7ADK7_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator Rua1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_030250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264142.1">
        <property type="nucleotide sequence ID" value="XM_009265867.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ADK7"/>
    <dbReference type="GeneID" id="20520934"/>
    <dbReference type="KEGG" id="ero:EROM_030250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030250"/>
    <dbReference type="HOGENOM" id="CLU_128868_0_0_1"/>
    <dbReference type="OrthoDB" id="5595379at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028012">
        <property type="entry name" value="Rua1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28125">
        <property type="entry name" value="MEIOTIC EXPRESSION UP-REGULATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28125:SF2">
        <property type="entry name" value="MEIOTIC EXPRESSION UP-REGULATED PROTEIN 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14616">
        <property type="entry name" value="Rua1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator Rua1 C-terminal" evidence="1">
        <location>
            <begin position="75"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN82645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="176" mass="20575" checksum="2E0BC5DD19B43FB2" modified="2012-10-03" version="1">MSSERSVLYLDDFLVLDSSMCGMTFDPQEFYELDNTHGFTELIDKRLFQTVVRTDDISDLPIHPREIRRQIGSGDMYKPRKVRGVGRLREGCCGICNSWFKLKTSSYWYHMNYKHGISSKGIKYPEPQIRYTGDKVDGFCMICDEWITLGYKSNRKSTRFGWFRHCQKKHGSGKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>I7AEL9</accession>
    <name>I7AEL9_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">DNA replication licensing factor MCM5</fullName>
            <ecNumber evidence="10">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">EROM_060250</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="10"/>
    <dbReference type="EMBL" id="CP003523">
        <property type="protein sequence ID" value="AFN83120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264617.1">
        <property type="nucleotide sequence ID" value="XM_009266342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AEL9"/>
    <dbReference type="GeneID" id="20521422"/>
    <dbReference type="KEGG" id="ero:EROM_060250"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_060250"/>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_1"/>
    <dbReference type="OrthoDB" id="10036721at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VI"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031261">
        <property type="term" value="C:DNA replication preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17756">
        <property type="entry name" value="MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000501">
        <property type="entry name" value="DNA replication licensing factor MCM7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008048">
        <property type="entry name" value="MCM5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF42">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01661">
        <property type="entry name" value="MCMPROTEIN5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="11">
        <location>
            <begin position="280"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="AFN83120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="696" mass="78798" checksum="57DBE870A714250C" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>I7AFD1</accession>
    <name>I7AFD1_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RSE1/DDB1/CPSF1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">EROM_071590</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003524">
        <property type="protein sequence ID" value="AFN83410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264907.1">
        <property type="nucleotide sequence ID" value="XM_009266632.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AFD1"/>
    <dbReference type="GeneID" id="20521723"/>
    <dbReference type="KEGG" id="ero:EROM_071590"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_071590"/>
    <dbReference type="HOGENOM" id="CLU_310369_0_0_1"/>
    <dbReference type="OrthoDB" id="436637at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VII"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050358">
        <property type="entry name" value="RSE1/DDB1/CFT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004871">
        <property type="entry name" value="RSE1/DDB1/CPSF1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10644">
        <property type="entry name" value="DNA REPAIR/RNA PROCESSING CPSF FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03178">
        <property type="entry name" value="CPSF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RSE1/DDB1/CPSF1 C-terminal" evidence="1">
        <location>
            <begin position="722"/>
            <end position="899"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="AFN83410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="933" mass="107561" checksum="5F683DB33C00F89A" modified="2012-10-03" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>I7AGD6</accession>
    <name>I7AGD6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nop domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_100070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265322.1">
        <property type="nucleotide sequence ID" value="XM_009267047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AGD6"/>
    <dbReference type="GeneID" id="20564437"/>
    <dbReference type="KEGG" id="ero:EROM_100070"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100070"/>
    <dbReference type="HOGENOM" id="CLU_1069693_0_0_1"/>
    <dbReference type="OrthoDB" id="2192281at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nop" evidence="2">
        <location>
            <begin position="107"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN83825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="260" mass="29879" checksum="2064A87FFE4D27EA" modified="2012-10-03" version="1">MDDTIQVYEENLKELYKMKDEIKSMLFLDDDLECLGHYPTVCCIITSLNGGIMEELQDNPHAIEMEMIITIGRSLDPDERKDLISKLEKIKAVAIEVDENRDILRVRLKYLHNLLGDDLFFKFITEVRSIFLLTRAIPSEVMKYGTKTYGSPLLSQHPLVAKASPKNQEKLLRKLCCKVTIAAKLDFCGSNFETDFSKQMEEVFNNLESNSKQEGSVLRVPLARKETRRGGIKARRKRSKSSPQKRRKFSKLEPDGNDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>I7ALI6</accession>
    <name>I7ALI6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone H2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">EROM_020640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003519">
        <property type="protein sequence ID" value="AFN82539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264036.1">
        <property type="nucleotide sequence ID" value="XM_009265761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ALI6"/>
    <dbReference type="GeneID" id="20520824"/>
    <dbReference type="KEGG" id="ero:EROM_020640"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_020640"/>
    <dbReference type="HOGENOM" id="CLU_062828_6_0_1"/>
    <dbReference type="OrthoDB" id="9421954at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome II"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="2">
        <location>
            <begin position="28"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="AFN82539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="126" mass="13923" checksum="E0A2FCDF8E7B14D9" modified="2012-10-03" version="1">MVQAKGGKADPRVMGKDEEHHKSIVKLSQIKKIMKDRTRMRISKDALVAVSACVMYLISEITDGAKNVANTDGKKKVMPKHINHAICNDTELHFVGHDWLIKNGGMKSYISPGDFSVSSKKGSSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>I7AME0</accession>
    <name>I7AME0_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_041020</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003521">
        <property type="protein sequence ID" value="AFN82869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264366.1">
        <property type="nucleotide sequence ID" value="XM_009266091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AME0"/>
    <dbReference type="GeneID" id="20521166"/>
    <dbReference type="KEGG" id="ero:EROM_041020"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_041020"/>
    <dbReference type="HOGENOM" id="CLU_083948_0_0_1"/>
    <dbReference type="OrthoDB" id="2191236at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN82869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="278" mass="32190" checksum="D581522919998E3E" modified="2012-10-03" version="1">MLILTNISREMNFEASKVNGRVFLKGDFEGIEYKGSEWNSPCYYCVVESSNGVIDLGRAEEARIVVFETVYETGVSHKKTSLKELAFTFGGKRLAGYHRRKEIDGYSKRVFTSSFKFKDQILPPMDEEAECPKKRFVLERMFPKEVLSSFEEIKVDSVLLHPDLMGLKQNMSYKTHFLLADCIIKLMEKKYITNDCLNDSGYSSFFAVIENEVENKMLTPLCRDKLAILCYILFLQIQGFSVKYEVLPDFKFSKKKVISMHRLIGCLYYSQSDTFKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AML5</accession>
    <name>I7AML5_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">EROM_050260</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">SJ-2008</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Non-catalytic component of the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264456.1">
        <property type="nucleotide sequence ID" value="XM_009266181.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AML5"/>
    <dbReference type="GeneID" id="20521258"/>
    <dbReference type="KEGG" id="ero:EROM_050260"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050260"/>
    <dbReference type="HOGENOM" id="CLU_072435_1_0_1"/>
    <dbReference type="OrthoDB" id="10248542at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03760">
        <property type="entry name" value="proteasome_beta_type_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016295">
        <property type="entry name" value="Proteasome_beta4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF6">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001213">
        <property type="entry name" value="Psome_endopept_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="AFN82959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="224" mass="25336" checksum="7BD9357599775622" modified="2012-10-03" version="1">MRNFVTGTSVISFKYKDGVMMGADTRGSYGRLAKYSGIQRMFKFGDQTLIGISGEVSDMQYLVKTLSILTEEDNRGIDPKGYHKIVQRMLYSARSKIRPLNLSVCVGGTRCDDDSMTHCERKMLGCVDRLGNFYFSDVICTGIGSYIALPFLRSKVEGREDEITREEGINLIEETMRILCYRDCNASNEIQIGYSDSQGVHISDPYPLKTNWDIGIREDEIVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMN8</accession>
    <name>I7AMN8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transcriptional activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_050620</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN82994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264491.1">
        <property type="nucleotide sequence ID" value="XM_009266216.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMN8"/>
    <dbReference type="GeneID" id="20521294"/>
    <dbReference type="KEGG" id="ero:EROM_050620"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_050620"/>
    <dbReference type="HOGENOM" id="CLU_086741_0_0_1"/>
    <dbReference type="OrthoDB" id="6365676at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF19">
        <property type="entry name" value="YY2 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="217"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="151"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN82994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="278" mass="32528" checksum="E1AECB2A7C835F6D" modified="2012-10-03" version="1">MLKNGVEMDRRRLDFSDESSDDGHDNGYLGFHEHNSRRVGTYEREVGKRSENLISSRHHACGGGYTGEVDSVGKKSHNVAQQENRGNSRLDTPVSIEGKDPHYSSEYFEASDSEEFEEKLASLRFLILEAEEELRNIIRGRIEKHLEANKEANERKREMKRRKREVLGEINKEESKEGNSPEISQEDSDSRREKIESFADFETSMFPYKSKNGIKSYICPYEGCTMELPTLSRIKRHYIVHTKLRPFKCLNKDCNKRFSRKDNMLQHYKIHCSYSNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>I7AMU8</accession>
    <name>I7AMU8_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="9">U3 small nucleolar ribonucleoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">EROM_051390</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003522">
        <property type="protein sequence ID" value="AFN83069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264566.1">
        <property type="nucleotide sequence ID" value="XM_009266291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AMU8"/>
    <dbReference type="GeneID" id="20521370"/>
    <dbReference type="KEGG" id="ero:EROM_051390"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_051390"/>
    <dbReference type="HOGENOM" id="CLU_097281_0_0_1"/>
    <dbReference type="OrthoDB" id="10248812at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome V"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034457">
        <property type="term" value="C:Mpp10 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF1">
        <property type="entry name" value="U3 SMALL NUCLEOLAR RIBONUCLEOPROTEIN PROTEIN IMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="AFN83069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="180" mass="21237" checksum="8490F757A62A0D07" modified="2012-10-03" version="1">MRELKFHEKKLLKRVDFLEWKNTNTAREHLAIVRYGLRDREEYVKYNRIVGKIRKLAEALSRLKENDPIRSNVTKKLVSKLYNIGIISNRKLVDCTKVTVSSFCERRLPMVMKRIRMVPNFKDATRFVEHGHVRLGSKVIYDPSTIISKAMEDFVSWVDESKIKKKIDEFNGKNDDFDCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>I7ANR6</accession>
    <name>I7ANR6_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_080060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264926.1">
        <property type="nucleotide sequence ID" value="XM_009266651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ANR6"/>
    <dbReference type="GeneID" id="20521742"/>
    <dbReference type="KEGG" id="ero:EROM_080060"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_080060"/>
    <dbReference type="HOGENOM" id="CLU_1229919_0_0_1"/>
    <dbReference type="OrthoDB" id="2191494at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="CDD" id="cd17039">
        <property type="entry name" value="Ubl_ubiquitin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="224" mass="26182" checksum="C15EB968DE676BB9" modified="2012-10-03" version="1">MSFTLLYPDGKRWETIKKAALESVIKGTSEEEPIFLPNDSGAQKDYLKVRRSYARVVCQIERGEVYDFYKYHRRFRMKSHALSEFNRFKVIETLKENGPCYVFEDECYKEVSSGSGYKRCPFCNEDIGSERINDHLRTRECNLETQGKEENSVTVLFDKGIKTEYENLVVNCSSVRMLKKIIYDKTGISVSKQDVYRGDTLLKNSDSLNRETVTLKQKKRSQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>I7AQX1</accession>
    <name>I7AQX1_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Origin recognition complex subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">EROM_030910</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">SJ-2008</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDC6/cdc18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003520">
        <property type="protein sequence ID" value="AFN82712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009264209.1">
        <property type="nucleotide sequence ID" value="XM_009265934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AQX1"/>
    <dbReference type="GeneID" id="20521002"/>
    <dbReference type="KEGG" id="ero:EROM_030910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_030910"/>
    <dbReference type="HOGENOM" id="CLU_012774_5_0_1"/>
    <dbReference type="OrthoDB" id="1926878at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome III"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050311">
        <property type="entry name" value="ORC1/CDC6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763:SF26">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10763">
        <property type="entry name" value="CELL DIVISION CONTROL PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="25"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="AFN82712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="347" mass="40302" checksum="8ED51A7435478B92" modified="2012-10-03" version="1">MVVIGREEEYLKLERYLDIFLSTEAGGIVYISGVPGAGKTHTVLELMERRQVSYLFLNATELRMKREVYKWILTNLPCSSGSKRMHLMNLQQHFMECTLPHVIVIDEVDILVGKTQEVLYNVFDMPYLKNSKVLLFVISNTINLPERLFEPKVCSRIGGRRVNFMPYTSMQLCSMAEGHRMKRKCIELISKRIGAISGDVRKVKDVIDRVRESEKGEDAEVLDVDGIMRKMYAPVYTYCLQGLSFYQKAILFLFNESERERMKVNELYEEFISFCKRSDVKEVGFFEYLDLIEALVGYGILGCRNSGKEVLAMVLKEEVERSLECDNEYLEMFRRSGNRRWPKETSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>I7AT09</accession>
    <name>I7AT09_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ricin B lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_081710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003525">
        <property type="protein sequence ID" value="AFN83587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265084.1">
        <property type="nucleotide sequence ID" value="XM_009266809.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7AT09"/>
    <dbReference type="SMR" id="I7AT09"/>
    <dbReference type="GeneID" id="20521906"/>
    <dbReference type="KEGG" id="ero:EROM_081710"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_081710"/>
    <dbReference type="HOGENOM" id="CLU_1288887_0_0_1"/>
    <dbReference type="OrthoDB" id="2193698at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome VIII"/>
    </dbReference>
    <dbReference type="CDD" id="cd00161">
        <property type="entry name" value="beta-trefoil_Ricin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="21768" checksum="8C285C68EB60B360" modified="2012-10-03" version="1">MITYACMIGIAFGFTIVSRDNKTESLINRSGKAFMSSEGKAVDFKGEEITPGVKIFTDKSNNKVLDIEGSRTNLIFYARHGGANQRFSLIKDEGSGYYIKSGDKCIERGSTGNLYRTTCSSKPQQKFDVVYTPEDPEYKPPVEVPVATESSPTPNPSPQILIFNGGKKHRPHSHFNVNEESSSIFDESNGLSTNGLIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATE6</accession>
    <name>I7ATE6_ENCRO</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">EROM_090910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">SJ-2008</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP003527">
        <property type="protein sequence ID" value="AFN83707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265204.1">
        <property type="nucleotide sequence ID" value="XM_009266929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATE6"/>
    <dbReference type="GeneID" id="20564315"/>
    <dbReference type="KEGG" id="ero:EROM_090910"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_090910"/>
    <dbReference type="HOGENOM" id="CLU_717843_0_0_1"/>
    <dbReference type="OrthoDB" id="2193421at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome IXb"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="AFN83707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="364" mass="42638" checksum="AB6F1C63FFCDFC47" modified="2012-10-03" version="1">MMKPPLRDQVRALLGTKKNIIAIRAIEAVEAPADELAPTLGDRKNINQYVYSTYYYMYKMGLIGKDRFESAKEHFRCMKNYEKMFIDTENSYIISRIEADRRRDKTVKREFNKVMDTKEELRSLLVELPEITVEEAHRIKDGVCSVKSIFVIDLDPIFDISSLRCQAKEEASRRISEIRDILFCIDSRHPRICTLGAIGEEEKDGDNELITLRNFKRDRYQHFERIFNGPEKLKFTCTPLFYSIDRLLKNRVTRLYDSVVKLYRTMQTVYFISSRPLPQILAELLFFEYSSLDIRDVVSTMDAPFDVYKEIQKKGNMRVVAVGNSTFVSQESKNVYRIGYESFRRMVDGILQMENTMERSGEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-10-03" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>I7ATV0</accession>
    <name>I7ATV0_ENCRO</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">EROM_100960</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Encephalitozoon romaleae (strain SJ-2008)</name>
        <name type="common">Microsporidian parasite</name>
        <dbReference type="NCBI Taxonomy" id="1178016"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Fungi incertae sedis</taxon>
            <taxon>Microsporidia</taxon>
            <taxon>Unikaryonidae</taxon>
            <taxon>Encephalitozoon</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="12638" last="12643">
            <title>Gain and loss of multiple functionally related, horizontally transferred genes in the reduced genomes of two microsporidian parasites.</title>
            <authorList>
                <person name="Pombert J.-F."/>
                <person name="Selman M."/>
                <person name="Burki F."/>
                <person name="Bardell F.T."/>
                <person name="Farinelli L."/>
                <person name="Solter L.F."/>
                <person name="Whitman D.W."/>
                <person name="Weiss L.M."/>
                <person name="Corradi N."/>
                <person name="Keeling P.J."/>
            </authorList>
            <dbReference type="PubMed" id="22802648"/>
            <dbReference type="DOI" id="10.1073/pnas.1205020109"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">SJ-2008</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP003529">
        <property type="protein sequence ID" value="AFN83912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_009265409.1">
        <property type="nucleotide sequence ID" value="XM_009267134.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="I7ATV0"/>
    <dbReference type="GeneID" id="20564526"/>
    <dbReference type="KEGG" id="ero:EROM_100960"/>
    <dbReference type="VEuPathDB" id="MicrosporidiaDB:EROM_100960"/>
    <dbReference type="HOGENOM" id="CLU_014923_3_2_1"/>
    <dbReference type="OrthoDB" id="336885at2759"/>
    <dbReference type="Proteomes" id="UP000010094">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054300">
        <property type="entry name" value="OB_DPOA2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22062">
        <property type="entry name" value="OB_DPOA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha subunit B OB" evidence="7">
        <location>
            <begin position="69"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="6">
        <location>
            <begin position="181"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF22062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AFN83912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000010094"/>
        </source>
    </evidence>
    <sequence length="411" mass="46501" checksum="16A781B845E3025A" modified="2012-10-03" version="1">MDFDRQVFRTLSKRRCVRSCIRIKHKPRISNFYLKKKDKYEYVRHRLATMEPYFLELLGIGSFEPVNYVSDTSFYTYGIIMNPFGYKLDSKNIFIASSIDGSNDVIVKLNLEHLQRYSVFPGQIVAIKGKNGTGREITVEALHCIPVVDINSAETPSEERYVQPTISAFSGPYGIGPEFSILDRVLIEDVDVLILVGPFIDPLQNDTSESPGSMMERVFIPKLKEWLSRRSDSKVILVPSTNDMACLNVFPQGPIDISDERIVCVSNPCEFFLNEFLIAISSLDTALEISSEECFYDSKSENEKDACGKLLFGNDRLERISYHLIFQRTFLPVFPSMNVVSYSVPESISMDTAPDIYIIVSKLKYFCRNAGPSVVVNLGLPVKDAEKVVCHISLPETLGERRPVVEFGCLK</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>